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Journal articles on the topic 'Higher plant chloroplasts'

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1

Ghoshroy, Soumitra, and Wayne R. Fagerberg. "Light-detection system in higher-plant chloroplasts : Pigment mediated or energy related." Proceedings, annual meeting, Electron Microscopy Society of America 50, no. 2 (1992): 1668–69. http://dx.doi.org/10.1017/s0424820100132972.

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Light is the driving force of photosynthesis. Plants adapt to rapid changes in irradiance, quality and duration of the light environment by modulating the composition of the thylakoid membranes to make the best use of the available light energy. Each chloroplast contains a large amount of thylakoid membranes some of which may be arranged as stacks (granal thylakoids) and others as unstacked sacks (stromal thylakoids). Shaded chloroplasts develop more thylakoid surface area as compared to those growing in full sunlight. Conversion of sun-type chloroplasts to those of shade-types can occur quick
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2

Kandasamy, M. K., and R. B. Meagher. "Cytoskeleton-Organelle Interaction: Higher Plant Chloroplasts Are Contained In Actin Baskets And Attached To Actin Filaments And Bundles." Microscopy and Microanalysis 6, S2 (2000): 296–97. http://dx.doi.org/10.1017/s1431927600033973.

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Plant organelles, including the dominant chloroplasts, migrate intracellularly on cytoplasmic strands (Fig. 1A-D). The chloroplasts in the leaf cells orient and redistribute in response to light to ensure maximum photosynthetic productivity. Their orderly distribution is also essential for proper transmission of organelle genome during cell proliferation. The movement and positioning of chloroplasts have been suggested to be mediated by the actin and tubulin-based cytoskeleton in green algae and higher plants. However, the actin structures controlling these processes have not been clearly deli
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3

Osaki, Yasuhide, and Yutaka Kodama. "Particle bombardment and subcellular protein localization analysis in the aquatic plant Egeria densa." PeerJ 5 (September 7, 2017): e3779. http://dx.doi.org/10.7717/peerj.3779.

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Particle bombardment is a powerful and relatively easy method for transient expression of genes of interest in plant cells, especially those that are recalcitrant to other transformation methods. This method has facilitated numerous analyses of subcellular localization of fluorescent fusion protein constructs. Particle bombardment delivers genes to the first layer of plant tissue. In leaves of higher plants, epidermal cells are the first cell layer. Many studies have used the epidermal cell layer of onion bulb (Allium cepa) as the experimental tissue, because these cells are relatively large.
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4

Chen, Shu Hsing, and Cheng Ho Li. "Investigating the Dynamics of Organelle-Actin Assembly with Dielectrophoresis." Materials Science Forum 594 (August 2008): 443–51. http://dx.doi.org/10.4028/www.scientific.net/msf.594.443.

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Dielectrophoresis is a technique of using high-frequency electric fields to manipulate dielectric particles. This work applies this technique to study the changes of intracellular properties in response to environmental changes. In a number of plant cells, chloroplasts change their positions for optimizing photosynthetic light absorption. Only between 2 and 20% of the light energy absorbed by algae and higher plants is actually used in CO2 fixation. Comparing with other micro-manipulative methods, dielectrophoresis is a relative simple method for investigating the microscopic mechanisms involv
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5

Sutton, C. A., O. V. Zoubenko, M. R. Hanson, and P. Maliga. "A plant mitochondrial sequence transcribed in transgenic tobacco chloroplasts is not edited." Molecular and Cellular Biology 15, no. 3 (1995): 1377–81. http://dx.doi.org/10.1128/mcb.15.3.1377.

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RNA editing occurs in two higher-plant organelles, chloroplasts and mitochondria. Because chloroplasts and mitochondria exhibit some similarity in editing site selection, we investigated whether mitochondrial RNA sequences could be edited in chloroplasts. We produced transgenic tobacco plants that contained chimeric genes in which the second exon of a Petunia hybrida mitochondrial coxII gene was under the control of chloroplast gene regulatory sequences. coxII transcripts accumulated to low or high levels in transgenic chloroplasts containing chimeric genes with the plastid ribosomal protein g
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6

Gounaris, K., J. Barber, and J. L. Harwood. "The thylakoid membranes of higher plant chloroplasts." Biochemical Journal 237, no. 2 (1986): 313–26. http://dx.doi.org/10.1042/bj2370313.

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7

Ahmad, Niaz, and Brent L. Nielsen. "Plant Organelle DNA Maintenance." Plants 9, no. 6 (2020): 683. http://dx.doi.org/10.3390/plants9060683.

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Plant cells contain two double membrane bound organelles, plastids and mitochondria, that contain their own genomes. There is a very large variation in the sizes of mitochondrial genomes in higher plants, while the plastid genome remains relatively uniform across different species. One of the curious features of the organelle DNA is that it exists in a high copy number per mitochondria or chloroplast, which varies greatly in different tissues during plant development. The variations in copy number, morphology and genomic content reflect the diversity in organelle functions. The link between th
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8

Vierling, E., L. M. Harris, and Q. Chen. "The major low-molecular-weight heat shock protein in chloroplasts shows antigenic conservation among diverse higher plant species." Molecular and Cellular Biology 9, no. 2 (1989): 461–68. http://dx.doi.org/10.1128/mcb.9.2.461.

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Several plant species are known to synthesize low-molecular-weight nucleus-encoded heat shock proteins (HSPs) which localize to chloroplasts. DNA sequence analysis of chloroplast HSP cDNAs from pea (Pisum sativum) and soybean (Glycine max) has shown that the carboxyl-terminal halves of these proteins are homologous to low-molecular-weight HSPs from a wide range of eucaryotes (E. Vierling, R. T. Nagao, A. E. DeRocher, and L. M. Harris, EMBO J. 7:575-581, 1988). We used a pea cDNA to construct fusion proteins containing either the carboxyl-terminal heat shock domain or the amino-terminal domain
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9

Vierling, E., L. M. Harris, and Q. Chen. "The major low-molecular-weight heat shock protein in chloroplasts shows antigenic conservation among diverse higher plant species." Molecular and Cellular Biology 9, no. 2 (1989): 461–68. http://dx.doi.org/10.1128/mcb.9.2.461-468.1989.

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Several plant species are known to synthesize low-molecular-weight nucleus-encoded heat shock proteins (HSPs) which localize to chloroplasts. DNA sequence analysis of chloroplast HSP cDNAs from pea (Pisum sativum) and soybean (Glycine max) has shown that the carboxyl-terminal halves of these proteins are homologous to low-molecular-weight HSPs from a wide range of eucaryotes (E. Vierling, R. T. Nagao, A. E. DeRocher, and L. M. Harris, EMBO J. 7:575-581, 1988). We used a pea cDNA to construct fusion proteins containing either the carboxyl-terminal heat shock domain or the amino-terminal domain
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10

Bard, J., D. P. Bourque, M. Hildebrand, and D. Zaitlin. "In vitro expression of chloroplast genes in lysates of higher plant chloroplasts." Proceedings of the National Academy of Sciences 82, no. 12 (1985): 3983–87. http://dx.doi.org/10.1073/pnas.82.12.3983.

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11

Paul, Puneet, Anida Mesihovic, Palak Chaturvedi, et al. "Structural and Functional Heat Stress Responses of Chloroplasts of Arabidopsis thaliana." Genes 11, no. 6 (2020): 650. http://dx.doi.org/10.3390/genes11060650.

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Temperature elevations constitute a major threat to plant performance. In recent years, much was learned about the general molecular mode of heat stress reaction of plants. The current research focuses on the integration of the knowledge into more global networks, including the reactions of cellular compartments. For instance, chloroplast function is central for plant growth and survival, and the performance of chloroplasts is tightly linked to the general status of the cell and vice versa. We examined the changes in photosynthesis, chloroplast morphology and proteomic composition posed in Ara
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12

Tang, Ning, Yumei Xia, Yijie Zhan, et al. "Improvement of Chloroplast Transformation Using CRISPR/Cas9." Journal of Biobased Materials and Bioenergy 14, no. 3 (2020): 401–7. http://dx.doi.org/10.1166/jbmb.2020.1970.

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Chloroplasts are organelles that contain genetic materials (DNA) in higher plant cells. The special genetic characteristics of chloroplasts mean that plasmid transformation has important research value, so it has become an important research direction second to nuclear transformation. Although the techniques of chloroplast genome modification have been successfully applied in tobacco and extended to other high plants, there are still many limitations. Exogenous genes are integrated into the chloroplast genome through homologous recombination. Therefore, the low efficiency of homologous recombi
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13

Roper, Jennifer M., and Alison G. Smith. "Molecular Localisation of Ferrochelatase in Higher Plant Chloroplasts." European Journal of Biochemistry 246, no. 1 (1997): 32–37. http://dx.doi.org/10.1111/j.1432-1033.1997.t01-1-00032.x.

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14

Miyamoto, Tetsuya, Junichi Obokata, and Masahiro Sugiura. "Recognition of RNA Editing Sites Is Directed by Unique Proteins in Chloroplasts: Biochemical Identification of cis-Acting Elements and trans-Acting Factors Involved in RNA Editing in Tobacco and Pea Chloroplasts." Molecular and Cellular Biology 22, no. 19 (2002): 6726–34. http://dx.doi.org/10.1128/mcb.22.19.6726-6734.2002.

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ABSTRACT RNA editing in higher-plant chloroplasts involves C-to-U conversions at specific sites. Although in vivo analyses have been performed, little is known about the biochemical aspects of chloroplast editing reactions. Here we improved our original in vitro system and devised a procedure for preparing active chloroplast extracts not only from tobacco plants but also from pea plants. Using our tobacco in vitro system, cis-acting elements were defined for psbE and petB mRNAs. Distinct proteins were found to bind specifically to each cis-element, a 56-kDa protein to the psbE site and a 70-kD
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15

El-Kafafi, El-Sayed, Mohamed Karamoko, Isabelle Pignot-Paintrand, et al. "Developmentally regulated association of plastid division protein FtsZ1 with thylakoid membranes in Arabidopsis thaliana." Biochemical Journal 409, no. 1 (2007): 87–94. http://dx.doi.org/10.1042/bj20070543.

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FtsZ is a key protein involved in bacterial and organellar division. Bacteria have only one ftsZ gene, while chlorophytes (higher plants and green alga) have two distinct FtsZ gene families, named FtsZ1 and FtsZ2. This raises the question of why chloroplasts in these organisms need distinct FtsZ proteins to divide. In order to unravel new functions associated with FtsZ proteins, we have identified and characterized an Arabidopsis thaliana FtsZ1 loss-of-function mutant. ftsZ1-knockout mutants are impeded in chloroplast division, and division is restored when FtsZ1 is expressed at a low level. F
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16

Linden, Hartmut, M. Mercedes Lucas, Maria Rosario de Felipe, and Gerhard Sandmann. "Immunogold localization of phytoene desaturase in higher plant chloroplasts." Physiologia Plantarum 88, no. 2 (1993): 229–36. http://dx.doi.org/10.1034/j.1399-3054.1993.880204.x.

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17

Linden, Hartmut, M. Mercedes Lucas, Maria Rosario Felipe, and Gerhard Sandmann. "Immunogold localization of phytoene desaturase in higher plant chloroplasts." Physiologia Plantarum 88, no. 2 (1993): 229–36. http://dx.doi.org/10.1111/j.1399-3054.1993.tb05493.x.

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18

Tan, Dun-Xian, and Russel J. Reiter. "An evolutionary view of melatonin synthesis and metabolism related to its biological functions in plants." Journal of Experimental Botany 71, no. 16 (2020): 4677–89. http://dx.doi.org/10.1093/jxb/eraa235.

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Abstract Plant melatonin research is a rapidly developing field. A variety of isoforms of melatonin’s biosynthetic enzymes are present in different plants. Due to the different origins, they exhibit independent responses to the variable environmental stimuli. The locations for melatonin biosynthesis in plants are chloroplasts and mitochondria. These organelles have inherited their melatonin biosynthetic capacities from their bacterial ancestors. Under ideal conditions, chloroplasts are the main sites of melatonin biosynthesis. If the chloroplast pathway is blocked for any reason, the mitochond
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19

Rose, RJ, MR Thomas, and JT Fitter. "The Transfer of Cytoplasmic and Nuclear Genomes by Somatic Hybridisation." Functional Plant Biology 17, no. 3 (1990): 303. http://dx.doi.org/10.1071/pp9900303.

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For somatic hybridisation between two species to be successful, specific regenerability, compatibility and selection criteria must be met. The development of new methodologies has reduced the reliance on auxotrophic and albino mutants in selection strategies. Somatic hybridisation allows the transfer of chloroplast, mitochondrial or nuclear genomes in a single-step procedure and can extend the transfer boundaries defined by sexual hybridisation. Cytoplasmic genomes can be transferred over wider genetic distances than nuclear genomes. We consider the transfer of the three genomes but with parti
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20

Robinson, SP, and WJS Downton. "Potassium, Sodium and Chloride Ion Concentrations in Leaves and Isolated Chloroplasts of the Halophyte Suaeda australis R. Br." Functional Plant Biology 12, no. 5 (1985): 471. http://dx.doi.org/10.1071/pp9850471.

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Seedlings of S. australis were grown hydroponically in nutrient solutions with 0-600 mM NaCl added. The plants grew poorly in the absence of added NaCl and optimal growth, based on fresh or dry weight, occurred at 50-150 mM NaCl. Higher concentrations of NaCl decreased growth although the plants continued to grow even at 600 mM NaCl. The osmotic potential of the leaf sap decreased with increasing salinity and this was mostly the result of accumulation of NaCl in the leaves. Leaf K+ concentration decreased with increasing salinity while both Na+ and Cl- increased, reaching 800 and 500 mM, respe
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21

Fambrough, Kristine, and Soumitra Ghoshroy. "Drought Induced Structural Changes in Developing Chloroplasts of Jalapeno Pepper (Capsicum annuum)." Microscopy and Microanalysis 7, S2 (2001): 66–67. http://dx.doi.org/10.1017/s1431927600026404.

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Chloroplasts, present in leaves of higher plants have an extensive, folded network of photosynthetic membranes. These membranes have closely appressed (grana) and non-appressed (stroma) regions and they are responsible for the conversion of solar energy into biochemically useful forms. Light plays an important role for the development of mature chloroplasts from proplastids. However, when seeds germinate in the dark, the proplastids do not form mature chloroplasts and instead they form etioplasts. The etioplasts contain a compact, highly regular lattice of inner membranes called prolamellar bo
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22

Ancín, María, Luis Larraya, Igor Florez-Sarasa, et al. "Overexpression of thioredoxin m in chloroplasts alters carbon and nitrogen partitioning in tobacco." Journal of Experimental Botany 72, no. 13 (2021): 4949–64. http://dx.doi.org/10.1093/jxb/erab193.

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Abstract In plants, there is a complex interaction between carbon (C) and nitrogen (N) metabolism, and its coordination is fundamental for plant growth and development. Here, we studied the influence of thioredoxin (Trx) m on C and N partitioning using tobacco plants overexpressing Trx m from the chloroplast genome. The transgenic plants showed altered metabolism of C (lower leaf starch and soluble sugar accumulation) and N (with higher amounts of amino acids and soluble protein), which pointed to an activation of N metabolism at the expense of carbohydrates. To further delineate the effect of
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23

Chen, Q., L. M. Lauzon, A. E. DeRocher, and E. Vierling. "Accumulation, stability, and localization of a major chloroplast heat-shock protein." Journal of Cell Biology 110, no. 6 (1990): 1873–83. http://dx.doi.org/10.1083/jcb.110.6.1873.

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Diverse higher plant species synthesize low molecular weight (LMW) heat shock proteins (HSPs) which localize to chloroplasts. These proteins are homologous to LMW HSPs found in the cytoplasm of all eukaryotes, a class of HSPs whose molecular mode of action is not understood. To obtain basic information concerning the role of chloroplast HSPs, we examined the accumulation, stability, tissue specificity, and intra-chloroplast localization of HSP21, the major LMW chloroplast HSP in pea. Intact pea plants were subjected to heat stress conditions which would be encountered in the natural environmen
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24

Ye, Guang-Ning, Henry Daniell, and John C. Sanford. "Optimization of delivery of foreign DNA into higher-plant chloroplasts." Plant Molecular Biology 15, no. 6 (1990): 809–19. http://dx.doi.org/10.1007/bf00039421.

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25

Glenz, Karin, Bernadette Bouchon, Thomas Stehle, Reinhard Wallich, Markus M. Simon, and Heribert Warzecha. "Production of a recombinant bacterial lipoprotein in higher plant chloroplasts." Nature Biotechnology 24, no. 1 (2005): 76–77. http://dx.doi.org/10.1038/nbt1170.

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26

Barber, Jim. "New organism for elucidating the origin of higher plant chloroplasts." Trends in Biochemical Sciences 11, no. 6 (1986): 234. http://dx.doi.org/10.1016/0968-0004(86)90180-5.

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27

Tsudzuki, Takahiko, Tatsuya Wakasugi, and Masahiro Sugiura. "Comparative Analysis of RNA Editing Sites in Higher Plant Chloroplasts." Journal of Molecular Evolution 53, no. 4-5 (2001): 327–32. http://dx.doi.org/10.1007/s002390010222.

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28

Lamppa, G. K., and M. S. Abad. "Processing of a wheat light-harvesting chlorophyll a/b protein precursor by a soluble enzyme from higher plant chloroplasts." Journal of Cell Biology 105, no. 6 (1987): 2641–48. http://dx.doi.org/10.1083/jcb.105.6.2641.

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A processing activity has been identified in higher plant chloroplasts that cleaves the precursor of the light-harvesting chlorophyll a/b-binding protein (LHCP). A wheat LHCP gene previously characterized (Lamppa, G.K., G. Morelli, and N.-H. Chua, 1985. Mol. Cell Biol. 5:1370-1378) was used to synthesize RNA and subsequently the labeled precursor polypeptide in vitro. Incubation of the LHCP precursors with a soluble extract from lysed chloroplasts, after removal of the thylakoids and membrane vesicles, resulted in the release of a single 25-kD peptide. In contrast, when the LHCP precursors wer
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29

Wu, Guochun, Sha Li, Xiaochuan Li, et al. "A Functional Alternative Oxidase Modulates Plant Salt Tolerance in Physcomitrella patens." Plant and Cell Physiology 60, no. 8 (2019): 1829–41. http://dx.doi.org/10.1093/pcp/pcz099.

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Abstract Alternative oxidase (AOX) has been reported to be involved in mitochondrial function and redox homeostasis, thus playing an essential role in plant growth as well as stress responses. However, its biological functions in nonseed plants have not been well characterized. Here, we report that AOX participates in plant salt tolerance regulation in moss Physcomitrella patens (P. patens). AOX is highly conserved and localizes to mitochondria in P. patens. We observed that PpAOX rescued the impaired cyanide (CN)-resistant alternative (Alt) respiratory pathway in Arabidopsis thaliana (Arabido
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30

Song, Qiping, Lili You, Yang Liu, Jiang Zhang, and Xinghong Yang. "Endogenous accumulation of glycine betaine confers improved low temperature resistance on transplastomic potato plants." Functional Plant Biology 47, no. 12 (2020): 1105. http://dx.doi.org/10.1071/fp20059.

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Glycine betaine (GB) plays a crucial role in plant response to abiotic stress, and its accumulation in chloroplasts is more effective than in the cytosol in improving the resistance of transgenic plants. Here, we report that the codA gene from Arthrobacter globiformis, which encodes a choline oxidase catalysing the conversion of choline to GB, was successfully introduced into the plastid genome of potato (Solanum tuberosum L.). Transgenic plants with plastid expression of codA showed increased tolerance to low temperature stress compared with the wild type (WT). Further studies revealed that u
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31

Dinç, Emine, Szilvia Z. Tóth, Gert Schansker, et al. "Synthetic Antisense Oligodeoxynucleotides to Transiently Suppress Different Nucleus- and Chloroplast-Encoded Proteins of Higher Plant Chloroplasts." Plant Physiology 157, no. 4 (2011): 1628–41. http://dx.doi.org/10.1104/pp.111.185462.

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32

Stern, D. B., and K. L. Kindle. "3'end maturation of the Chlamydomonas reinhardtii chloroplast atpB mRNA is a two-step process." Molecular and Cellular Biology 13, no. 4 (1993): 2277–85. http://dx.doi.org/10.1128/mcb.13.4.2277.

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Inverted repeat (IR) sequences are found at the 3' ends of most chloroplast protein coding regions, and we have previously shown that the 3'IR is important for accumulation of atpB mRNA in Chlamydomonas reinhardtii (D. B. Stern, E.R. Radwanski, and K. L. Kindle, Plant Cell 3:285-297, 1991). In vitro studies indicate that 3' IRs are inefficient transcription termination signals in higher plants and have furthermore defined processing activities that act on the 3' ends of chloroplast transcripts, suggesting that most chloroplast mRNAs are processed at their 3' ends in vivo. To investigate the me
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33

Stern, D. B., and K. L. Kindle. "3'end maturation of the Chlamydomonas reinhardtii chloroplast atpB mRNA is a two-step process." Molecular and Cellular Biology 13, no. 4 (1993): 2277–85. http://dx.doi.org/10.1128/mcb.13.4.2277-2285.1993.

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Inverted repeat (IR) sequences are found at the 3' ends of most chloroplast protein coding regions, and we have previously shown that the 3'IR is important for accumulation of atpB mRNA in Chlamydomonas reinhardtii (D. B. Stern, E.R. Radwanski, and K. L. Kindle, Plant Cell 3:285-297, 1991). In vitro studies indicate that 3' IRs are inefficient transcription termination signals in higher plants and have furthermore defined processing activities that act on the 3' ends of chloroplast transcripts, suggesting that most chloroplast mRNAs are processed at their 3' ends in vivo. To investigate the me
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34

Krzeszowiec, Weronika, Maria Novokreshchenova, and Halina Gabryś. "Chloroplasts in C3 grasses move in response to blue-light." Plant Cell Reports 39, no. 10 (2020): 1331–43. http://dx.doi.org/10.1007/s00299-020-02567-3.

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Abstract Key message Brachypodium distachyonis a good model for studying chloropla st movements in the crop plants, wheat, rye and barley. The movements are activated only by blue light, similar to Arabidopsis. Abstract Chloroplast translocations are ubiquitous in photosynthetic organisms. On the one hand, they serve to optimize energy capture under limiting light, on the other hand, they minimize potential photodamage to the photosynthetic apparatus in excess light. In higher plants chloroplast movements are mediated by phototropins (phots), blue light receptors that also control other light
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35

Sugiura, M., M. N. Georgescu, and M. Takahashi. "A Nitrite Transporter Associated with Nitrite Uptake by Higher Plant Chloroplasts." Plant and Cell Physiology 48, no. 7 (2007): 1022–35. http://dx.doi.org/10.1093/pcp/pcm073.

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36

Nakamura, T., G. Schuster, M. Sugiura, and M. Sugita. "Chloroplast RNA-binding and pentatricopeptide repeat proteins." Biochemical Society Transactions 32, no. 4 (2004): 571–74. http://dx.doi.org/10.1042/bst0320571.

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Chloroplast gene expression is mainly regulated at the post-transcriptional level by numerous nuclear-encoded RNA-binding protein factors. In the present study, we focus on two RNA-binding proteins: cpRNP (chloroplast ribonucleoprotein) and PPR (pentatricopeptide repeat) protein. These are suggested to be major contributors to chloroplast RNA metabolism. Tobacco cpRNPs are composed of five different proteins containing two RNA-recognition motifs and an acidic N-terminal domain. The cpRNPs are abundant proteins and form heterogeneous complexes with most ribosome-free mRNAs and the precursors of
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37

Huner, N. P. A. "Acclimation of winter rye to cold-hardening temperatures results in an increased capacity for photosynthetic electron transport." Canadian Journal of Botany 63, no. 3 (1985): 506–11. http://dx.doi.org/10.1139/b85-063.

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Photosynthetic electron transport and its partial reactions were measured as a function of light intensity and temperature in isolated chloroplasts from cold-hardened and unhardened rye (Secale cereale L. cv. Puma). Chloroplasts from cold-hardened rye plants exhibited light-saturated rates for whole chain electron transport that were about 1.4-fold higher at 25 °C than those observed in chloroplasts from unhardened rye plants. This was correlated with light-saturated rates of electron transport through photosystem I that were 1.6-fold higher in cold-hardened chloroplasts than in unhardened chl
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38

Sugita, Mamoru, and Masahiro Sugiura. "Regulation of gene expression in chloroplasts of higher plants." Plant Molecular Biology 32, no. 1-2 (1996): 315–26. http://dx.doi.org/10.1007/bf00039388.

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39

Bednarz, J., A. Radunz, and G. H. Schmid. "Lipid Composition of Photosystem I and II in the Tobacco Mutant Nicotiana tabacum NC 95." Zeitschrift für Naturforschung C 43, no. 5-6 (1988): 423–30. http://dx.doi.org/10.1515/znc-1988-5-617.

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The lipids of photosystem II particles, of chloroplasts and leaves are compared in the variegated tobacco mutant NC 95. The mutant differs from other N. tabacum mutants by the phenomenon that it has variegated leaves with green and with yellow-green leaf patches. Chloroplasts from the green leaf areas exhibit photosystem II and photosystem I reactions and have a normal lamellar system with grana and intergrana regions. Chloroplasts from the yellow-green leaf areas, however, yield only photosystem I reactions and have only single stranded isolated thylakoids. Hence, this mutant offers the uniqu
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40

Timmis, JN, and MA Ayliffe. "Extranuclear DNA and its use in systematics." Australian Systematic Botany 3, no. 1 (1990): 137. http://dx.doi.org/10.1071/sb9900137.

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Higher plant cells contain three genetic compartments which separately transcribe DNA and translate messenger (m)RNA into polypeptides. Most plant proteins derive from nuclear genes and their mRNAs are translated on ribosomes in the cytoplasm. Many fewer genes are located within mitochondrial (mt) or plastid (including chloroplast (cp)) DNA, and mRNAs from these genomes are translated on separate populations of ribosomes within the particular organelles. The cytoplasmic genomes are much smaller than that of the nucleus and they are present in multiple copies in each organelle. As there are man
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41

Mohanta, Tapan Kumar, Awdhesh Kumar Mishra, Adil Khan, Abeer Hashem, Elsayed Fathi Abd_Allah, and Ahmed Al-Harrasi. "Gene Loss and Evolution of the Plastome." Genes 11, no. 10 (2020): 1133. http://dx.doi.org/10.3390/genes11101133.

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Chloroplasts are unique organelles within the plant cells and are responsible for sustaining life forms on the earth due to their ability to conduct photosynthesis. Multiple functional genes within the chloroplast are responsible for a variety of metabolic processes that occur in the chloroplast. Considering its fundamental role in sustaining life on the earth, it is important to identify the level of diversity present in the chloroplast genome, what genes and genomic content have been lost, what genes have been transferred to the nuclear genome, duplication events, and the overall origin and
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Madhavan, S., M. S. Miller-Goodman, and K. W. Lee. "Immunolocalization of Rubisco Activase and Rubisco in C3 and C4 Plant Tissues." Microscopy and Microanalysis 6, S2 (2000): 472–73. http://dx.doi.org/10.1017/s1431927600034851.

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Ribulose bisphosphate carboxylase/oxygenase (Rubisco), an abundant enzyme in chloroplasts, must be activated by CO2 in order for it to catalyze the carboxylation of ribulose bisphosphate. Rubisco activase, a nuclear encoded chloroplast protein was first identified as a biochemical lesion in the rca mutant of Arabidopsis (1) which lacked this enzyme. Study of Rubisco in this mutant (2) and transgenic tobacco plants with reduced Rubisco activase levels showed that Rubisco could not achieve and maintain an adequate level of activity, in vivo, without an activase. Rubisco activase promotes ‘activa
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Quinn, P. J. "Lipid unsaturation and the organization of photosynthetic complexes in higher-plant chloroplasts." Biochemical Society Transactions 25, no. 3 (1997): 1080–88. http://dx.doi.org/10.1042/bst0251080.

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Ostersetzer, Oren, Sarit Tabak, Oded Yarden, Roni Shapira, and Zach Adam. "Immunological Detection of Proteins Similar to Bacterial Proteases in Higher Plant Chloroplasts." European Journal of Biochemistry 236, no. 3 (1996): 932–36. http://dx.doi.org/10.1111/j.1432-1033.1996.00932.x.

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Ladygin, V. G. "Lutein-5,6-epoxide aycle: A new xanthophyll cycle in higher plant chloroplasts." Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology 2, no. 2 (2008): 110–18. http://dx.doi.org/10.1134/s1990747808020037.

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Ladygin, V. G. "“Lutein-5,6-epoxide cycle: A new xanthophyll cycle in higher plant chloroplasts”." Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology 3, no. 3 (2009): 342. http://dx.doi.org/10.1134/s1990747809030520.

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Krupa, Zbigniew. "Cadmium against Higher Plant Photosynthesis -a Variety of Effects and Where Do They Possibly Come From?" Zeitschrift für Naturforschung C 54, no. 9-10 (1999): 723–29. http://dx.doi.org/10.1515/znc-1999-9-1017.

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The complexity of in vivo toxic effects of Cd on higher plants makes almost impossible an accurate distinction between direct and indirect mechanisms of its action on the photosynthetic apparatus. We, therefore, postulate that multiple Cd effects on plant physiological and metabolic processes may finally be focused on photosynthesis. This would also explain the phenomenon that only a small fraction of Cd entering chloroplasts may cause such disastrous changes in their structure and function. In return, the inhibition of photosynthesis affects numerous metabolic pathways dependent on the primar
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Liu, Youqi, and Nancy G. Dengler. "Bundle sheath and mesophyll cell differentiation in the C4 dicotyledon Atriplex rosea: quantitative ultrastructure." Canadian Journal of Botany 72, no. 5 (1994): 644–57. http://dx.doi.org/10.1139/b94-085.

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In leaves of most C4 species, both bundle sheath and mesophyll cells are derived from ground meristem, yet at maturity differ in photosynthetic enzyme complement and in cell size, shape, and subcellular ultrastructure. This quantitative ultrastructural study of bundle sheath and mesophyll cell differentiation in Atriplex rosea shows that while developmental pathways of bundle sheath and meosphyll cells are generally coordinated, the timing of developmental divergence differs among individual characteristics. For instance, bundle sheath cells are larger, with more chloroplasts and more and larg
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Macadlo, Lauren A., Iskander M. Ibrahim, and Sujith Puthiyaveetil. "Sigma factor 1 in chloroplast gene transcription and photosynthetic light acclimation." Journal of Experimental Botany 71, no. 3 (2019): 1029–38. http://dx.doi.org/10.1093/jxb/erz464.

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Abstract Sigma factors are dissociable subunits of bacterial RNA polymerase that ensure efficient transcription initiation from gene promoters. Owing to their prokaryotic origin, chloroplasts possess a typical bacterial RNA polymerase together with its sigma factor subunit. The higher plant Arabidopsis thaliana contain as many as six sigma factors for the hundred or so of its chloroplast genes. The role of this relatively large number of transcription initiation factors for the miniature chloroplast genome, however, is not fully understood. Using two Arabidopsis T-DNA insertion mutants, we sho
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Wieckowski, S., and M. Bojko. "The NADPH-dependent electron flow in chloroplasts of the higher plants." Photosynthetica 34, no. 4 (1998): 481–96. http://dx.doi.org/10.1023/a:1006836706867.

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