Academic literature on the topic 'Hinton, S. E. Outsiders'
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Journal articles on the topic "Hinton, S. E. Outsiders"
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Werth, Brian, Kelsi Penewit, Stephen Salipante, Tianwei Shen, Libin Xu, and Abhinav Nath. "712. Identification of a Novel Tedizolid Resistance Mutation in rpoB of Methicillin-Resistant Staphylococcus aureus." Open Forum Infectious Diseases 5, suppl_1 (2018): S256. http://dx.doi.org/10.1093/ofid/ofy210.719.
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Abstract Background Tedizolid (TDZ) is an oxazolidinone antimicrobial with broad-spectrum activity against Gram-positive bacteria including methicillin-resistant S. aureus (MRSA). Resistance to TDZ is uncommon but mutations in the 23S rRNA target as well as in the transferable rRNA methyltransferase gene cfr, which also mediate resistance to linezolid and chloramphenicol have been implicated. The objective of this study was to determine whether other TDZ resistance pathways exist in MRSA. Methods Using a well-characterized MRSA strain, N315, we selected for TDZ resistance by serial passage in escalating concentrations of TDZ in Mueller Hinton broth (MHB) starting with 0.5× the MIC. Once visible growth was achieved a sample of the broth was diluted 1:1,000 into fresh MHB with twice the previous concentration of TDZ until an isolate with an MIC of ≥4 mg/mL was recovered. This MIC was selected since it is 1 dilution above the breakpoint for resistance ≥2 mg/L). This isolate was subjected to whole genome sequencing (WGS) and MICs to other antimicrobials were assessed. Homology modeling was performed to evaluate the potential impact of the mutation on target protein function. Results After 10 days of serial passage we recovered a stable mutant with a TDZ MIC of 4 mg/L. WGS revealed a single nucleotide variant (A1345G) in the rpoB gene corresponding to an amino acid substitution at D449N. The following table and figure summarize the changes in drug susceptibility between the parent and evolved strain and reveals the location of the amino acid substitution relative to the TDZ binding site. Conclusion We have identified a novel mutation in the RNA polymerase gene, rpoB, that mediates oxazolidinone and chloramphenicol resistance. This variant lies outside of the rifampin resistance determinant clusters of rpoB that span from 1,384 to 1,464 and 1,543 to 1,590, and as expected did not affect rifampin susceptibility. The underlying molecular mechanism by which this single nucleotide variant confers TDZ resistance remains unclear but may involve transcriptional modulation by altered sigma factor binding. Disclosures All authors: No reported disclosures.
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Gillespie, Joanne S. "Getting inside S. E. Hinton's "The Outsiders"." English Journal 95, no. 3 (2006): 44. http://dx.doi.org/10.2307/30047043.
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Stout, B. "Hinton, M. S. and Newburn, T. (2008). * POLICING DEVELOPING DEMOCRACIES." Policing 5, no. 4 (2011): 377–78. http://dx.doi.org/10.1093/police/par015.
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Kuminskaya, E. A. "The Outsider Phenomenon in Teenage Groups." Psychological-Educational Studies 9, no. 2 (2017): 84–95. http://dx.doi.org/10.17759/psyedu.2017090208.
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The article is devoted to the problem of outsidership in groups of younger and older adolescents. We assumed that there are personal qualities that distinguish outsiders from other students, which allows u s t o s peak o f o utsidership a s a s eparate g roup a nd p ersonal phenomenon. The study involved 246 students of Moscow schools of younger and older adolescents, 60 of whom took an outsider position in the group. To test the hypothesis were used: a personal questionnaire for adolescents HSPQ (R. Cattell), questionnaire Emin to determine the level of emotional intelligence (D.V. Lyusin), the technique of " Suggestibility " (O.E. Rybakov). To identify statistical differences, the Student's t-test and the Mann-Whitney Utest were used, p ≤ 0.05. The results of the study s howed that outsiders of adolescence are indeed characterized by a number of personal characteristics that distinguish them from their peers. Differences in personal characteristics of outsiders at different stages of adolescence have their own characteristics.
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Horan, John J., and Chris D. Erickson. "Fellowship Behavior in Division 17 and the MOMM Cartel." Counseling Psychologist 19, no. 2 (1991): 253–59. http://dx.doi.org/10.1177/0011000091192011.
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Graduates and employees of four institutions-the MOMM Cartel-dominate every science and practice organ of Division 17's governing body. Counseling psychologists from the other 60 academic programs (the OUTSIDERs) face numerous barriers to professional ascendancy. Six of 13 fellow nominees during the 1988-1989 year were MOMM members; none were rejected. Three OUTSIDERs were elected; four were rejected. Mean scholarly productivity for each group was 13, 21.3, and 19.7 Psyc LIT citations, respectively. The accepted OUTSIDERs were significantly more productive than the MOMMs; two of the four rejected OUTSIDERs ranked numbers one and two in scholarly productivity among all nominees. No relationship appeared between scholarship and fellow decisions; MOMM membership strongly predicted election to fellow status. Personal familiarity with the evaluators, rather than professional service, apparently accounts for these filings -a variant of the "Matthew Effect" discussed in the sociology-of-science literature Recommendations for reform are offered
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FERNANDES, ANDRÉ S., MARIA INÊS S. PASSOS, and NEUSA HAMADA. "Stegoelmis Hinton, 1939 (Coleoptera: Elmidae: Elminae) in Brazil: two new species and a key to the Brazilian species." Zootaxa 2921, no. 1 (2011): 56. http://dx.doi.org/10.11646/zootaxa.2921.1.6.
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Two new species of the genus Stegoelmis Hinton, 1939, S. figueiredoensis sp. nov. from Amazonas State, Brazil and S. shepardi sp. nov. from Roraima State, Brazil and Saint Laurent Du Maroni, French Guiana, are described and illustrated based on adult specimens. This work also provides a taxonomic key to males of Stegoelmis species known from Brazil.
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Assegaf, Samira, Arthur Pohan Kawilarang, and Retno Handajani. "Antibacterial Activity Test of Red Ginger Extract (Zingiber officinale var. Rubrum) Against Streptococcus pyogenes In vitro." Biomolecular and Health Science Journal 3, no. 1 (2020): 24. http://dx.doi.org/10.20473/bhsj.v3i1.19130.
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Introduction: Red ginger (Zingiber officinale var. rubrum) is known as an antibacterial agent. Streptococcus pyogenes is the most common bacterial cause of pharyngitis in children and adults. Over the years, antibiotic resistance case is increasing. Therefore, further research of other substances to find an bactericidal or bacteriostatic agent is needed. The aim of this study is to determine the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of Z. officinale var. rubrum against S. pyogenes. Methods: Serial dilution test of red ginger extract in Mueller hinton broth (8 concentrations of red ginger extract : 80%, 40%, 20%, 10%, 5%, 2,5%, 1,25%, and 0,625%) and positive control (1 ml bacterial suspension in Mueller hinton broth) was used to determine MIC. MBC was determined by culturing solutions from the previous dilution test into blood agar plate.lResults: MIC of red ginger extract against S. pyogenes could not be determined because the color of red ginger extract affected the turbidity of the dilution test result. MBC of red ginger extract against S. pyogenes was at concentration 20% of red ginger extract.Conclusion: Z. officinale var. rubrum showed antibacterial activity against S. pyogenes.
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Gardam, M. A., and M. A. Miller. "Optochin Revisited: Defining the Optimal Type of Blood Agar for Presumptive Identification of Streptococcus pneumoniae." Journal of Clinical Microbiology 36, no. 3 (1998): 833–34. http://dx.doi.org/10.1128/jcm.36.3.833-834.1998.
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To determine the optimal media for optochin susceptibility testing of Streptococcus pneumoniae, we measured inhibition zones for 72 S. pneumoniae and 22 Streptococcus viridans isolates on three blood-containing media. Because 15.3, 0, and 22.2% of S. pneumoniae organisms were misidentified on Columbia agar, Trypticase soy agar (TSA), and Mueller-Hinton agar, respectively, each containing sheep blood, we recommend that TSA-sheep blood agar be used.
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Johnson, Bethany, and Margaret M. Quinlan. "Insiders and Outsiders and Insider(s) Again in the (In)fertility World." Health Communication 32, no. 3 (2016): 381–85. http://dx.doi.org/10.1080/10410236.2016.1138384.
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Yanik, Keramettin, Emin Guluzade, Kemal Bilgin, et al. "Ceftaroline activity on certain respiratory tract and wound infection agents at the minimum inhibitory concentration level." Journal of Infection in Developing Countries 9, no. 10 (2015): 1086–90. http://dx.doi.org/10.3855/jidc.6300.
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Introduction: The aim of this study was to investigate the effectiveness of ceftaroline against agents frequently isolated from respiratory tract and wound infections. Methodology: The study included a total of 250 strains isolated from various clinical specimens, among which were Streptococcus pyogenes, Streptococcus agalactiae, Streptococcus dysagalactiae, Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catharralis. The bacteria were identified using the matrix-assisted laser desorption/ionization time-of-flight method and conventional methods. The bacteria’s antibiotic susceptibility was tested using appropriate broth microdilution. Mueller-Hinton broth with 4% lysed horse blood, Haemophilus test medium broth, and Mueller-Hinton broth were used. Ceftaroline fosamil results at the minimum inhibitory concentration (MIC) were evaluated using Clinical and Laboratory Standards Institute (CLSI) criteria. For quality assurance, E. coli ATCC 35218, S. aureus ATCC 29213, S. aureus ATCC 43300, S. pneumoniae ATCC 49619, H. influenzae ATCC 49766, H. influenzae ATCC 10211, and H. influenzae ATCC 49247 standard strains were used. Results: According to CLSI criteria, resistance was not detected in any strains. Due to the absence of CLSI criteria for M. catharralis, the susceptibility state for this bacterium was not evaluated. The various strains’ MIC50–MIC90 values were as follows: for S. pyogenes, 0.015–0.06; for S. agalactiae, 0.03–0.125; for S. dysagalactiae, 0.03–0.06; for S. pneumoniae, 0.06–0.125; for H. influenzae, 0.015–0.125; and for M. catharralis, 0.5–1. Conclusions: The results indicate that ceftaroline is quite effective against bacteria that are frequently isolated from respiratory tract and wound infections.
Dissertations / Theses on the topic "Hinton, S. E. Outsiders"
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Roy, Aurélie. "Surviving childhood : trauma and maturation in J.D. Salinger's "The catcher in the Rye", S.E. Hinton's "The outsiders", and Stephen Chbosky's "The perks of being a wallflower"." Master's thesis, Université Laval, 2017. http://hdl.handle.net/20.500.11794/28334.
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Ce mémoire de maîtrise propose une étude détaillée des répercussions sociales et psychologiques des évènements traumatiques sur le développement de l’enfant en utilisant trois Bildungsromane importants, The Catcher in the Rye de J.D. Salinger, The Outsiders de S.E. Hinton et The Perks of Being a Wallflower de Stephen Chbosky. En me basant sur les études de Cathy Caruth, Shoshana Felman, Ronald Granofsky, Dominick LaCapra, Dori Laub et Judith Lewis Herman, je démontre que les expériences traumatiques ont des effets contradictoires, mais indissociables sur l’évolution psychologique de l’enfant, à savoir de ralentir et d’accélérer le passage de l’enfance au monde adulte. J’explique que Holden Caulfield, Ponyboy Curtis et Charlie font preuve de comportements qui dévoilent la paralysie que peuvent entraîner les traumatismes psychologiques et qui peut forcer l’enfant à demeurer immature et innocent. Ensuite, j’illustre que ces mêmes expériences traumatiques, et l’acceptation de celles-ci peuvent finir par pousser l’enfant à brusquement atteindre la maturité. Ce mémoire souligne également l’importance du témoignage dans la guérison et dans l’éventuel passage de l’enfance à l’âge adulte. Considérant son omniprésence dans les romans pour jeunes adultes, le témoignage et ses ramifications seront analysés dans chaque chapitre. Au fil des trois chapitres, j’analyse chaque roman de façon indépendante, j’évalue la réaction de chaque personnage face aux expériences traumatiques et j’établis la nomenclature et les liens qui permettent une conversation entre les trois romans. Bien que les trois œuvres étudiées proposent différents modèles de réponses psychologiques aux traumatismes, chaque roman entre en dialogue avec l’autre. L’objectif de ce mémoire est donc d’offrir de nouvelles perspectives sur les trois histoires, tout en démontrant les corrélations qui les unissent et qui contribuent à la compréhension de chaque œuvre. Ainsi, ce mémoire se veut un outil pour établir une conception globale du rôle des expériences traumatiques dans la littérature pour jeunes lecteurs.This thesis examines the psychological and social repercussions of trauma on a child’s maturation process using three landmark novels of the Bildungsroman genre, J.D. Salinger’s The Catcher in the Rye, S.E. Hinton’s The Outsiders, and Stephen Chbosky’s The Perks of Being a Wallflower. Grounding my analysis on the findings of trauma theorists Cathy Caruth, Shoshana Felman, Ronald Granofsky, and Dominick LaCapra, and psychiatrists Dori Laub and Judith Lewis Herman, I demonstrate that trauma can occupy two contradictory but inextricably linked functions in the maturation process, that of both hindering and catalyzing a child’s coming-of-age. I demonstrate that Holden Caulfield, Ponyboy Curtis, and Charlie all display behaviours that suggest the paralyzing nature of trauma and its initial ability to prevent the child from growing up. I then argue that trauma, or the acceptance of one’s traumatic past, has the potential to accelerate the maturation process. My study also highlights the importance of the testimonial process in the recovery from trauma and the character's ensuing maturity. Because of the omnipresence of testimony in young people’s literature, its ramifications and implications are explored in each chapter of this thesis. Through the use of close reading, I study each work independently, evaluate each character’s individual response to trauma, and establish the thematic vocabulary and the interlinks that allow a conversation between the three novels. Although the studied works propose different types of traumatic negotiation and response, each novel is dialogically linked with the other. This thesis offers new readings of each novel, while establishing insightful comparisons between the three works. The intent of this thesis is therefore to contribute to the assessment of each narrative and to a general understanding of the role of trauma in young people’s literature.
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Manguinho, Julyana Vilar de Fran?a. "Arte, prazer e bisturi: constru??o corporal atrav?s da body modification." Universidade Federal do Rio Grande do Norte, 2012. http://repositorio.ufrn.br:8080/jspui/handle/123456789/12279.
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Previous issue date: 2012-03-30Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior
In view of a field research carried out by a team connected to the universe of body modification, it is possible to discern some uses and meanings linked to these forms of body interventions. Body modification or body change is part of the circuit of piercings and tattoos, although they are socially less thinned and more extreme, like scarifications, subcutaneous implants, bifurcated tongues, surfaces and body suspensions. The aim of this paper is to cast an anthropological glance on these practices, joining at the same context all the subjects involved with these techniques, placing them inside the same relational focus and capturing their journeys and trajectories. The discussions are concentrated on the notion of body building and urban life style. Ideas as personal distinctness and prestige imitation are also present in this universe, as well as matters attached to genre, pleasure, art, and to the so-called alternative circuit . This way, the ethnography so far presented here, reveals the complex and contemporaneous character of these practices of body markings in which the body appears as the central element in the experiences of the subjects of this study
Diante da pesquisa de campo realizada com um grupo ligado ao universo da body modification, ? poss?vel perceber alguns usos e significados associados a essas formas de interven??es corporais. A body modification ou modifica??o corporal faz parte do circuito dos piercings e tatuagens, no entanto, s?o formas menos dilu?das socialmente e consideradas mais extremas, como: escarifica??es, alargadores, implantes subcut?neos, l?ngua bifurcada, surfaces e suspens?es corporais. O objetivo desta disserta??o ? lan?ar um olhar antropol?gico sobre essas pr?ticas, contextualizando os sujeitos envolvidos com essas t?cnicas, situando-os dentro de um enfoque relacional, percebendo seus percursos e trajet?rias. As discuss?es est?o centradas em torno da no??o de constru??o corporal e estilo de vida na cidade. Ideais como distin??o pessoal e imita??o prestigiosa tamb?m est?o presentes nesse universo, bem como quest?es ligadas a g?nero, prazer, arte, e ao chamado circuito alternativo . Dessa forma, a etnografia aqui apresentada demonstra o car?ter complexo e contempor?neo dessas pr?ticas de marca??es corporais, em que o corpo aparece como elemento central nas experi?ncias dos sujeitos desta pesquisa
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Ruiz, Gabriela, Sebastián García, Córdova Lucía Mercado, and Estelí Vela. "Party system strength in the 80’s and the rise of anti-politics in the 90’s in Peru: a quantitative descriptive analysis of subnational level." Politai, 2013. http://repositorio.pucp.edu.pe/index/handle/123456789/92034.
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This paper explores two important topics among the political parties and party system literature in Peru: The strength and stability of the political party system in the 80’s and the decade of the 90’s, better known as the «decade of the antipolitics». The contrast between the literature reviewed and the quantitative data from the 7 Municipal Elections, that took place among this time period, attempts to find if this phenomena occurred also at the subnational level. In the decade of the 80’s, the political party system stayed stable with rule of the four most important political parties (APRA, Acción Popular, Izquierda Unida y PPC); however, individually these political parties did not have the same performance. In the decade of the 90’s, the concept «in- dependent» did not fit equally among all the cases and together with the fujimorismo depicted the political scenario of the 90’s.El presente artículo explora dos temas importantes en la literatura sobre partidos políticos y sistema de partidos en el Perú: la fortaleza y estabilidad del sistema de partidos en la década de los 80 y la década de los 90, más conocida como la «década de la antipolítica». El contraste entre la literatura revisada y los datos cuantitativos de las 7 elecciones municipales que tuvieron lugar a lo largo de este período de tiempo, propone buscar si dichos fenómenos se presentaron también en el nivel subnacional. En la década de los 80, el sistema de partidos políticos se mantuvo estable con el dominio de los cuatro grandes partidos (APRA, Acción Popular, Izquierda Unida y PPC); sin embargo, individualmente estos no tuvieron el mismo desempeño. En la década de los 90, la definición de «independiente» no encajó igualmente en todos los casos y junto con el fujimorismo caracterizaron el escenario político de los 90.
Books on the topic "Hinton, S. E. Outsiders"
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Understanding The outsiders. Lucent Books, 2001.
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Franklin, Joseph. S. E. Hinton. Rosen Publishing, 2016.
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Daly, Jay. Presenting S.E. Hinton. Twayne Publishers, 1987.
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Daly, Jay. Presenting S.E. Hinton. Twayne Publishers, 1989.
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Daly, Jay. Presenting S.E. Hinton. Twayne Publishers, 1989.
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Rijksuniversiteit te Groningen. Afdeling Spaans. Symposium Internacional. Marginalismo-s: Actas del 3o Symposium International del Departamento de Español de la Universidad de Groningen 15, 16, y 17 de octubre de 1984. Rijksuniversiteit Groningen, Vakgroep Spaanse Taal-en Letterkunde, 1990.
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S. E. Hinton: Author of The Outsiders (Authors Teens Love). Enslow Publishers, 2007.
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E, Hinton S. An S. E. Hinton Collection. Viking Juvenile, 2001.
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Hull, Starr L. The outsiders by S.E. Hinton: Study guide (Novel units). Novel Units, 1989.
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S. E. Hinton: Prebound Paperback Set. Baker & Taylor, 1989.
Book chapters on the topic "Hinton, S. E. Outsiders"
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Butcher, Kevin. "Information, Legitimation, or Self-Legitimation? Popular and Elite Designs on the Coin Types of Syria." In Coinage and Identity in the Roman Provinces. Oxford University Press, 2005. http://dx.doi.org/10.1093/oso/9780199265268.003.0017.
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In a Famous Essay ‘Numismatics and History’, A. H. M. Jones suggested that Roman imperial coin types could be compared to the designs on modern postage stamps. The purpose of this analogy was to demonstrate the relative insignificance of types and legends for the study of imperial policy. In doing so, however, he addressed a fundamental problem which is of particular relevance here: What are the meaning(s) of coin types, and who chose the designs? Jones’s comparison was perhaps offered with a slight hint of facetiousness, as a way of debunking the notion that imperial history could be reconstructed from the coin designs alone, yet the analogy does have some merit when considering the meanings of types on Roman provincial coins. These do indeed depict ‘famous men of the country concerned, its artistic monuments . . . fairs . . . or . . . great events in national history’, among other things. The analogy is not intended to mean that the designs were empty or frivolous, or that people could not construct a sense of identity from them. This chapter examines some of the coin types of cities in Syria, to show that not only the more unusual types, but also some of the designs that we might consider generic could have been seen as specific and generated a sense of identity among members of the issuing community. Whether individuals within a community found the same meanings in the designs is a question addressed here. What people understood is crucial to any search for identities. It is suggested that whilst people from outside the community might have understood the types, these designs were not generally intended to convey ‘information’ to outsiders. Some of the types may remain unexplained today because they were perhaps equally obscure to many non-citizens in antiquity. However, unless the issuing authorities and the audience can be clearly identified it will be very difficult to say very much about how the coin types generated a sense of identity, and what sort of identities were generated.
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Morse, Geoffrey, and Thomas Braithwaite. "4. Partners and Outsiders." In Partnership and LLP Law. Oxford University Press, 2020. http://dx.doi.org/10.1093/he/9780198832799.003.0004.
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This chapter sets out the vicarious liability of partners for the various forms of partnership liability to third parties. The liability for contracts is based on the concepts of agency and the authority of the partner(s) making the contract, in particular the scope of the implied or usual authority of a partner to bind the other partners. The exception to the undisclosed principal rule is carefully examined. The vicarious liability for torts and crimes committed by a partner is examined, including the apparent paradox of the non-existent legal person of the firm being subject to prosecution. The liability for breaches of equitable liabilities relating to misapplications by a partner is synthesised, including knowing receipt and dishonest assistance in a breach of trust. Then the chapter considers the nature of such liabilities and finally, the effect of a change of partners on such liabilities on both the outgoing and incoming partners.
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Thacker, Andrew. "Paris." In Modernism, Space and the City. Edinburgh University Press, 2019. http://dx.doi.org/10.3366/edinburgh/9780748633470.003.0002.
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This chapter explores the affective pull that Paris exerted upon modernist writers and artists, attracting outsiders from around the globe to experience its cultural institutions and openness to creative experimentation. The chapter first discusses the writers T. S. Eliot, Guillaume Apollinaire, and Blaise Cendrars as ‘outsider-insiders’ (in Peter Gay’s terms), figures who come to the city as outsiders but who, by virtue of status or identity, are able to function as insiders within its cultural geography. The second group of writers discusses include Hope Mirrlees (in her poem Paris), Jean Rhys (in novels such as After Leaving Mr Mackenzie and Good Morning, Midnight), and Gwendolyn Bennett (in her story ‘Wedding Day’), female modernists who remain marked as outsiders in the city. The chapter discusses how all of these writers engaged affectively with various aspects of the technological modernity of Paris, including features such as the Eiffel Tower, café culture, hotel rooms, and the Grands Boulevards.
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Thomas, Eric A. "The Futures Outside: Apocalyptic Epilogue Unveiled as Africana Queer Prologue." In Sexual Disorientations. Fordham University Press, 2017. http://dx.doi.org/10.5422/fordham/9780823277513.003.0004.
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This essay examines the epilogue of Revelation (22:8-21) as an intervention for new imaginations of, and actions toward, a new heaven and new earth that can be realized in the present. It names the ways that Revelation (indeed, the Bible) is used to make outsiders of queer people. More importantly it suggests that the author/narrator John is not the only one who can be filled with the spirit on the Lord(e)’s day with something to say to those “with ears to hear.” The particular “queer time and place” of this investigation occurs at the intersection of queers of color critique, theories of queer temporality, and Sankofa—the Akan concept that we take what is beneficial from the past in order to work toward a more pleasurable future. Composite sketches of the lives of queer folks in the African Diaspora are gathered to create a “deep archive” (following Judith Halberstam) from which Muñoz’s call for new visions of a utopian “then and there” can be articulated in resistance to their apocalyptic “here and now.” Consequently, a re-vision of the death-dealing epilogue can become a life-restoring prologue toward the enactment of Africana queer utopian futures outside of apocalyptic Christo-heteronormativity.
Conference papers on the topic "Hinton, S. E. Outsiders"
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Silva, Iago castro da, and Nilton Akio Muto. "AVALIAÇÃO DA CAPACIDADE ANTIMICROBIANA DE BACTÉRIAS ISOLADAS DO MEL DE ABELHA SEM FERRÃO NATIVA DA AMAZÔNIA SCAPTOTRIGONA AFF POSTICA." In I Congresso de Engenharia de Biotecnologia. Revista Multidisciplinar de Educação e Meio Ambiente, 2021. http://dx.doi.org/10.51189/rema/1371.
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O presente trabalho relata a capacidade antimicrobiana de méis de abelha Scaptotrigina aff. postica. O objetivo foi realizar o isolamento de bactérias com potencial antimicrobiano dos meis de S. aff. postica e testar a capacidade antimicrobiana frente aos patógenos B. cereus, E. coli, Salmonella sp. e S. aureus em comparativo com os méis de Appis mellifera. A partir disso foi utilizada a metodologia de Patel e Vakilwala (2016) que consistiu na filtração dos metabólitos produzidos pelas bactérias isoladas (screening) do mel. O procedimento consistiu na aplicação do inóculo bacteriano com turvação de 0.5 da escala de Mac Farland (Correspondente a 108 UFC/ml). O inóculo foi distribuído sobre as superfícies das placas de petri contendo meio Ágar Mueller Hinton. Foram feitos poços na superfície do ágar de 4 mm de diâmetro e retirados o ágar dos poços com a ajuda de uma pinça estéril. Os poços foram devidamente identificados de acordo com a cepa bacteriana, e 25 µL do filtrado bacteriano das colônias isoladas e cultivadas a partir dos méis de A. mellifera e de S. aff. postica foram dispensadas nos poços respectivos. Como controle positivo foi utilizado o antibiótico Estreptomicina (EST10) com 10 µg. Foram isoladas 9 cepas de ambos os méis para realizar o teste, sendo 7 cepas isoladas do mel de Scaptotrigona aff postica e 2 cepas isoladas de mel de Appis mellifera. Os resultados mostraram zonas de inibição baixas, moderadas e altas para as cepas isoladas, mostrando a viabilidade de caracterização do composto em futuras aplicações biotecnológicas.
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Garda, Carla Munique Aparecida, Vitoria Karolini Betim Fieldkircher Caus, Naiara Vitoria Ferreira Cortes Koprovski, Eduarda Carolina Scherer Hagemann, and Dalila Moter Benvegnú. "ATIVIDADE ANTIMICROBIANA DE EXTRATO HIDROALCOÓLICO DE CASCA DE NOZ PECÃ EM CEPAS DE STAPHYLOCOCCUS AUREUS ISOLADAS DE MASTITE BOVINA." In I Congresso Nacional de Microbiologia Clínica On-Line. Revista Multidisciplinar em Saúde, 2021. http://dx.doi.org/10.51161/rems/1196.
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Introdução: A mastite bovina, caracterizada pela inflamação da glândula mamária é um dos principais problemas que afeta a bovinocultura leiteira, cujo tratamento costuma ser realizado através da utilização de antibióticos, os quais podem acarretar riscos à saúde pública, considerando que nenhuma tecnologia é capaz de eliminar os resíduos presentes nos alimentos. O agente Staphylococcus aureus, um dos principais causadores dessa afecção, apresenta resistência significativa aos antibióticos convencionais. Assim, faz-se necessárias novas abordagens terapêuticas, nesse sentido, a fitoterapia tem se destacado. Entre as diversas espécies que têm sido estudadas, encontra-se a noz pecã. Estudos que buscam avaliar o efeito antimicrobiano da noz pecã têm instigado a comunidade científica na busca por resultados promissores. Objetivo: avaliar o potencial antibacteriano do extrato hidroalcoólico da casca de noz pecã sobre 25 cepas de S. aureus isoladas de casos de mastite bovina. Material e métodos: o extrato hidroalcoólico (1:1) foi obtido em uma concentração de 20%, utilizando um extrator Soxhlet. Para a avaliação antimicrobiana dos extratos, a técnica empregada foi a Concentração Inibitória Mínima (CIM), determinada pela técnica de microdiluição seriada, em microplacas (96 orifícios), sendo que no poço inicial, o conteúdo era de: 34,5 μl de extrato + 195 μl de caldo + 10 μl de inóculo. Os testes foram realizados em triplicatas e uma linha da microplaca foi destinada aos controles. A CIM foi avaliada pela leitura de absorbância em 605 nm no equipamento Elisa Multiskan FC (Thermo Scientific) e realizada a confirmação com revelador Resazurina. A determinação da concentração bactericida mínima (CBM) foi realizada em placas com meio ágar Mueller-Hinton. Resultados: Verificou-se que o extrato apresentou resultados de CIM e CBM correspondentes para todas as cepas, sendo que em 96% (n=24) na concentração de 15% de extrato, e em 4% das cepas (n=1) na concentração de 7.5% de extrato. Conclusão: Os resultados indicaram que o extrato hidroalcoólico exerceu efeito antimicrobiano sobre as cepas testadas. Assim, tendo em vista o efeito promissor da casca de noz pecã, espera-se que os achados possam instigar o desenvolvimento de novos estudos acerca do mecanismo de ação dessa substância no tratamento da mastite bovina.