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1
Buchs, Jean-Paul. "Das ABO-Histo-Blutgruppensystem /." Bern, 1991. http://www.ub.unibe.ch/content/bibliotheken_sammlungen/sondersammlungen/dissen_bestellformular/index_ger.html.
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Le, Bars Pierre. "Etude histo-immunologique de la stomatite prothetique." Nantes, 2000. http://www.theses.fr/2000NANT23VS.
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Barbé, Laure. "Spécificité d’attachement sur les glycannes, vers une amélioration des vaccins rotavirus." Thesis, Nantes, 2018. http://www.theses.fr/2018NANT1017/document.
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Les souches humaines de rotavirus du groupe A (RVA) reconnaissent des glycannes fucosylés de la famille des Histo-Blood Group Antigens (HBGAs) et des gangliosides via la protéine de capside VP8*. L’interaction avec les gangliosides est essentielle pour l’entrée cellulaire et l’absence de ligands fucosylés dû au polymorphisme génétique des HBGAs est associée à une résistance à la gastroentérite sévère. Nos objectifs étaient de délimiter la contribution des HBGAs et du ganglioside GM1a dans le processus d’infection et d’explorer les conséquences du polymorphisme des HBGAs sur la transmission du virus et l’efficacité des vaccins vivants disponibles.génoty Ces travaux ont permis de montrer la concordance entre la spécificité glycannique des VP8* P[8], génotype le plus fréquent en France, et la sensibilité HBGA-dépendante à la gastroentérite sévère. La reconnaissance des HBGAs par les souches humaines de RVA apparaît donc essentielle pour l’infection symptomatique. Néanmoins, nos résultats suggèrent que l’attachement aux HBGAs correspond à un événement précoce puisqu’il n’est pas nécessaire pour l’infection de cellules peu différenciées par les souches P[8] adaptées à la culture. La contribution du GM1a dans l’infection reste incertaine. Enfin, nous avons montré que la reconnaissance des HBGAs est conservée entre des souches P[8] récentes et anciennes, indiquant que le polymorphisme des HBGAs pourrait contribuer à expliquer le défaut d’efficacité des vaccins dans les régions où la fréquence d’individus n’exprimant pas les ligands fucosylés est élevéeHuman strains of rotavirus A (RVAs) recognize fucosylated glycans of the histo-blood group family (HBGAs) as well as gangliosides through the VP8* protein of their capsid. Interaction with gangliosides is essential for cell entry and lack of fucosylated ligands due to HBGAs genetic polymorphism is associated with resistance to RVA gastroenteritis. Our goals are to delineate the contribution of HBGAs and gangliosides in the infection process and to explore the consequences of HBGAs polymorphisms on the virus transmission and efficacy of the available live vaccines. This study highlighted the concordance between the glycan specificity of P[8] VP8*, the most common genotype in France, and the HBGA-dependant susceptibility to RVA gastroenteritis. The recognition of HBGAs by human RVA strains therefore appears essential to the infection. Yet, our results suggest that HBGA binding corresponds to an early event since it is not required for infection of poorly differentiated cells by cell culture-adapted P[8] strains. The contribution of GM1a on infection remains unclear. Finally, we showed that HBGA recognition is conserved between recent and older P[8] strains, suggesting that HBGAs polymorphism may contribute to explain the low efficacy of vaccines in areas where the frequency of individuals who do not express fucosylated ligands is high
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Miola, Marcos Paulo. "Resolução de discrepâncias do Sistema histo-sanguíneo ABO." Faculdade de Medicina de São José do Rio Preto, 2017. http://hdl.handle.net/tede/392.
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Introduction. ABO histo-blood group system is the most important transfusional system and the identification of its phenotypes is often performed by means of direct and reverse typing, which must always present concordant results. However, some genetic factors such as natural chimerisms and point mutations in the ABO gene may affect the expression of the antigens and antibodies of this system, contributing to the discrepancy in the phenotyping, requiring investigations to define the correct phenotype of receptors and blood donors. Objectives. The main objective of this study was to investigate the variations in the expression of the antigens of the ABO histo-blood group system. Its specific objectives were: 1. Selection of recipients and blood donors that presented discrepancies between the results of the direct and reverse phenotyping of the ABO histo-blood group system; 2. Investigation, using serological and molecular methods, of the causes of phenotypic changes and discrepancies between the results of direct and reverse phenotypes in the ABO histo-blood group system in the recipients and donors of blood. Material and Methods. Samples of recipients (n = 2) and blood donors (n = 7) presenting discrepancies between the direct and reverse phenotyping were selected. Phenotyping were performed using conventional and modified hemagglutination methods in tubes and gel columns with commercial antisera and lectins. Molecular investigations were performed using PCR-RFLP method and sequencing of exons 6 and 7 of the ABO gene and exon 2 of the FUT2 gene. Results. Four cases with poor expression of antigen A and absence of expected antibody, observed in hemagglutination, were identified as A2B, Ael and Aw. Four cases without antigenic alteration but carrying an irregular antibody anti-A1 or absence of expected antibody were characterized as AB, A1 and O and presented common ABO alleles. A case of non-dizygotic twins, phenotyped as AB and with double red blood cell population was characterized as hematopoietic chimera after extensive family analysis. The DNA extracted from buccal swab revealed the ABO (A101/B101) and FUT2 (SE*25.01.01/SE*25.01.01) genotypes in the male twin and the ABO (O01/O02) and FUT2 (SE*01.04.01/SE*01.06.03) genotypes in the female twin. Sequences of two new ABO (ABO*Aw.38; KT906366.1) and FUT2 (SE*01.06.03; KX550421) allele sequences were deposited on GenBank. Conclusions. Our results demonstrate that the use of serum and salivary serological assays combined with molecular methods are good tools to solving discrepancies between the direct and reverse phenotyping of the ABO histo-blood group system as well as elucidate cases of twin chimerism in humans, with a double population of red blood cells. In addition, they contribute to the identification of new alleles of the ABO and FUT2 genes.
Introdução. O sistema histo-sanguíneo ABO é o de maior importância transfusional e a identificação de seus fenótipos é frequentemente realizada por meios das tipagens direta e reversa as quais sempre devem apresentar resultados concordantes. Entretanto, alguns fatores genéticos como quimerismos naturais e mutações pontuais no gene ABO, podem afetar a expressão dos antígenos e anticorpos deste sistema, contribuindo com a discrepância nas fenotipagens, requerendo investigações para se definir o correto fenótipo de receptores e doadores de sangue. Objetivos. O objetivo geral deste estudo foi investigar as variações na expressão dos antígenos do sistema histo-sanguíneo ABO. Seus objetivos específicos compreenderam: 1. Seleção de receptores e doadores de sangue que apresentaram discrepâncias entre os resultados das fenotipagens direta e reversa do sistema histo-sanguíneo ABO; 2. Investigação, com o uso de métodos sorológicos e moleculares, das causas das alterações fenotípicas e discrepâncias entre os resultados das fenotipagens direta e reversa no sistema histo-sanguíneo ABO nos receptores e doadores de sangue. Material e Método. Foram selecionadas amostras de receptores (n=2) e doadores (n=7) de sangue com discrepâncias entre as fenotipagens direta e reversa. As fenotipagens foram realizadas com o uso dos métodos de hemaglutinação convencional e modificada, em tubos e colunas de gel, com antissoros comerciais e lectinas. As investigações moleculares foram realizadas com o uso dos métodos PCR-RFLP e sequenciamento dos exons 6 e 7 do gene ABO e do exon 2 do gene FUT2. Resultados: Quatro casos com fraca expressão do antígeno A e ausência do anticorpo esperado, observados na hemaglutinação, foram identificados como A2B, Ael e Aw. Quatro casos sem alteração antigênica, mas com presença de anticorpo irregular ou ausência do anticorpo esperado, foram caracterizados como AB, A1 e O e apresentaram alelos comuns. Um caso de gêmeos não dizigóticos, fenotipados como AB e com dupla população de hemácias foi caracterizado como quimera hematopoiética, após extensa análise familiar. O DNA extraído de swab bucal revelou os genótipos ABO (A101/B101) e FUT2 (SE*25.01.01/SE*25.01.01) no gêmeo masculino e os genótipos ABO (O01/O02) e FUT2 (SE*01.04.01/SE*01.06.03) no gêmeo feminino. As sequências de dois novos alelos dos genes ABO (ABO*Aw.38; KT906366.1) e FUT2 (SE*01.06.03; KX550421) foram depositadas no GenBank. Conclusões: Nossos resultados demonstram que o uso de análises sorológicas eritrocitárias e salivares combinadas a métodos moleculares são fundamentais na resolução de discrepâncias entre as fenotipagens direta e reversa do sistema histo-sanguíneo ABO bem como no esclarecimento de casos de quimerismo gemelar em humanos, contendo dupla população de hemácias. Além disso, contribuem para a identificação de novos alelos dos genes ABO e FUT2.
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Becker, Philippe. "Etude histo-enzymologique du muscle oculaire droit interne." Nancy 1, 1988. http://www.theses.fr/1988NAN11283.
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Vaglio, Giovanna. "Analyse histo-morphométrique des vaisseaux sanguins du ligament parodontal bovin /." Genève : [s.n.], 2008. http://opac.nebis.ch/cgi-bin/showAbstract.pl?sys=000253997.
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Brückner, Stella. "Histo- und cytochemische Untersuchung der Zwischenwirbelscheibe des Rhesusaffen (Macaca mulatta)." Diss., lmu, 2004. http://nbn-resolving.de/urn:nbn:de:bvb:19-18952.
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Luciene, Della Libera Miguel. "Associação entre o sistema histo-sanguíneo ABO e mucosite oral." Faculdade de Medicina de São José do Rio Preto, 2016. http://hdl.handle.net/tede/405.
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Introduction: Oral mucositis is one of the most frequent diseases resulting from the side effects of Chemotherapy and Radiotherapy. In addition to compromising the quality of life, it increases the risk of infections, especially in patients undergoing bone marrow transplantation. However, genetic risk factors related to the susceptibility to this disease have not been fully clarified. Objectives: The aim of this study was to verify whether there is an association between ABO blood group phenotyping and oral mucositis. Methods: Data were selected from two hundred twenty nine records of patients undergoing HSCT in the Unit of Bone Marrow Transplantation of Hospital de Base São José do Rio Preto; out from the underlying disease between March 2006 and March 2012. Group 1 (G1) comprised data from patients with mucositis demonstrations after HSCT; Group 2 (G2) comprised patients without data mucositis demonstrations after HSCT. The Chi-Square and Fisher Exact tests were used for comparison of proportions between patients with and without oral mucositis and other risk factors. The mean of ages was calculated using the t test. The values of Odds Ratio (OR) and confidence intervals (CI) of 95% were also calculated (p <0.05). Results: No statistically significant differences were observed in the frequency of erythrocyte phenotypes of the ABO blood group in patients with and without oral mucositis (χ2: 2.654, p = 0.448, DF = 3). Statistically significant differences were found between the frequencies of the ABO blood group phenotypes when comparisons were related to the type of transplantation, conditioning and degree of oral mucositis. Conclusion: ABO blood group is not associated to the occurrence of oral mucositis in patients undergoing bone marrow transplantation.
Introdução: Mucosite oral é uma das mais frequentes doenças resultantes dos efeitos colaterais de quimioterápicos e radioterápicos. Além de comprometer a qualidade de vida, aumenta os riscos de infecções, especialmente em pacientes submetidos ao transplante de medula óssea. Contudo, fatores de risco genéticos envolvidos na suscetibilidade a esta doença ainda não foram totalmente esclarecidos. Objetivos: O objetivo geral deste estudo foi verificar se há associação entre os fenótipos eritrocitários do sistema histo-sanguíneo ABO e a mucosite oral. Casuística e Método: Foram selecionados dados de duzentos e vinte nove prontuários de pacientes submetidos ao TCPH na Unidade de Transplante de Medula Óssea do Hospital de Base de São José do Rio Preto, independente da doença de base, entre Março de 2006 e Março de 2012. O grupo 1 (G1) compreendeu dados de pacientes com manifestações de mucosite, após o TCPH; o grupo 2 (G2) compreendeu dados de pacientes sem manifestações de mucosite, após o TCPH. Os testes exato de Fisher e qui-quadrado foram utilizados para comparação das proporções entre pacientes com e sem mucosite oral e outros fatores de risco. As médias de idade foram calculadas com o uso do teste t. Os valores de Odds Ratio (OR) e de intervalos de confiança (IC) a 95% também foram calculados (p<0,05). Resultados: Não foram observadas diferenças estatisticamente significantes nas frequências dos fenótipos eritrocitários do sistema histo-sanguíneo ABO em pacientes com e sem mucosite oral (χ2: 2.654, p = 0.448, GL = 3). Também não foram observadas diferenças estatisticamente significantes entre as frequências dos fenótipos eritrocitários ABO quando as comparações compreenderam tipo de transplante, condicionamento e graus de mucosite oral. Conclusão: O sistema histo-sanguíneo ABO não está associado à ocorrência de mucosite oral em pacientes submetidos ao transplante de medula óssea.
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Fernandes, Sandra Regina Muchinechi 1954. "Poliarterite nodosa : contribuição ao estudo clinico, laboratorial, histo-patologico e angiografico." [s.n.], 1993. http://repositorio.unicamp.br/jspui/handle/REPOSIP/310426.
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Orientador : Lilian Tereza Lavras CostallatTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Ciencias Medicas
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Resumo: Foram estudados 20 casos de Poliarterite Nodosa (PAN), quanto à freqüência das manifestações clínicas, alterações laboratoriais e dados histopatológicos e arteriográficos. A PAN sistêmica foi caracterizada em 16 casos e a PAN cutânea benigna em quatro. Em relação aos sintomas gerais na PAN sistêmica oito tiveram febre e 11 emagrecimento, sugerindo doença sistêmica, em oposição aos quatro restantes que não tinham essas evidências, mas apresentavam microaneurismas em artérias renais. Artralgia ou artrite esteve presente em oito casos e neuropatia periférica em seis. Envolvimento renal foi constatado em oito casos, sendo hipertensão arterial em sete, hematúria em cinco, proteinúria em quatro e insuficiência renal em três. Manifestações pulmonares, cardíacas e do sistema nervoso central foram infrequentes. A idade de inicio superior a 50 anos, a presença de manifestações musculares, gastrointestinais e hematúria foram elementos significativos nos casos que evoluiram ao óbito. No laboratório, os dados hematológicos foram inespecíficos revelando leucocitose, anemia e VHS elevado. De oito casos investigados dois apresentaram antígeno HBsAg. ANCA resultou negativo em todos os sete investigados. Cinco em seis biopsiados na pele evidencia~am dados histopatológicos de vasculite necrosante, sugerindo que este procedimento é importante para nortear o diagnóstico da PAN sistêmica, embora sem distingui-Ia da PAN cutânea. A biópsia muscular, revelou-se positiva apenas em dois de um total de cinco examinados. A histopatologia renal, foi a mais marcante, visto que todos os casos examinados revelaram vasculite necrosante extraglomerular não deixando dúvida quanto ao diagnóstico. Seis em onze casos apresentaram alterações à arteriografia renal, cinco com microaneurismas e um com diminuição do lume vascular. Não obstante este achado, três tinham cifras pressóricas normais e quatro sem evidências de alterações renais pela analise do sedimento urinário. A arteriografia renal foi o procedimento diagnóstico em três casos. A PAN cutânea revelou-se de evolução e prognóstico benignos nos quatro casos estudados. Nestes, os dados laboratoriais, sejam da doença ou das complicações viscerais apresentaram-se constantemente normais ou ausentes. Numa escala de valores de procedimentos diagnósticos, lado dos dados clínicos que, em sua maioria, se assemelhavam aos da literatura, a biópsia de pele lesada revelou-se o dado mais marcante seguida pela arteriografia renal
Abstract: Twenty patients with polyarteritis nodosa (PAN) were studied to determine the clinical, laboratorial, histologic and renal arteriogram features. Sixteen patients presented the systemic subset and four had the characteristics of cutaneous PAN. Constitutional symptoms such as fever and weight lass was observed in eight and 11 patients with systemic PAN respectively. Four patients didn't present these manifestations but had aneurysms in renal arteriograms. Joint involvement and peripheral neuropathy were common. Renal manifestations were observed in eight cases, seven of them with hypertension, urinary protein in four, hematuria in five and renal insufficiency in three. Pulmonary, cardiac and cerebrovascular involvement were rarely seem. Age of onset the disease superior to 50 years, gut involvement, hematuria and muscle manifestations were associated with a worse prognosis. Leukocytosis, anemia and elevated ESR were often detected, but were not diagnostic. Serum hepatitis B surface antigen determinations were performed in eight cases and in two were positive. ANCA was negative in alI seven performed. Six clinically involved skin was biopsied, and it revealed necrotizing vasculitis in alI but one. This procedure was important to make the diagnosis of a systemic PAN, although this histologic features is the same of cutaneous PAN. Skeletal muscle was biopsied in five cases and revealed vasculitis in only two. AlI renal samples showed extraglomerular necrotizing vasculitis. Renal arteriograms were performed in 11 patients and revealed aneurysms in five and reduced lume in another. Of these six cases, three didn't had hypertension and four had a normal urine sediment Renal arteriograms defined a diagnosis of systemic PAN in three cases. The cases of cutaneous PAN had a benign course, with exacerbations and remissions that never showed systemic involvement. The laboratorial findings were essentially normal
Doutorado
Doutor em Ciências Médicas
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Bulut, Murat. "Kinetics of methane adsorption on HiPco purified single-walled carbon nanotubes /." Available to subscribers only, 2008. http://proquest.umi.com/pqdweb?did=1594488051&sid=1&Fmt=2&clientId=1509&RQT=309&VName=PQD.
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MELLO, A. F. "El Elogio del Pueblo: a questão nacional na historiografia de José Victorino Lastarria." Universidade Federal do Espírito Santo, 2011. http://repositorio.ufes.br/handle/10/3456.
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Previous issue date: 2011-05-05O objetivo central deste estudo é analisar as relações entre representação histórica e identidade nacional nos escritos do chileno José Victorino Lastarria. Assim, buscamos rastrear os procedimentos através dos quais o pueblo-nación chileno é transformado no principal sujeito de suas narrativas históricas, a partir de quais dispositivos conceptuais ele procurou tornar a nação chilena uma entidade tangível, conformando, assim, um discurso sobre sua nacionalidade.
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Dahle, Knut Ove. "The susceptibility of grade 70 anchor chain steel to HISC." Thesis, Norges teknisk-naturvitenskapelige universitet, Institutt for materialteknologi, 2011. http://urn.kb.se/resolve?urn=urn:nbn:no:ntnu:diva-16341.
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Due to recent fractures in grade 70 anchor chains in the fish farming industry, chain supplier Erling Haug AS has initiated an examination of a sandblasted anchor they sell today. The sandblasted anchor chain is of the same strength category as the chains associated with the mentioned accidents. The mechanical properties of the sandblasted chain were characterized in 2010 in a student project work[1]. The report expressed concern about the sandblasted grade 70 chains since the material fulfilled requirements to be susceptible to hydrogen induced stress cracking (HISC). Therefore, further research on the material was needed. Another grade 70 chain, of an unknown origin, was obtained to make a comparison. This chain had fractured in-service. It was therefore necessary to characterize the mechanical properties of the fractured chain before examining the two chain grades susceptibility to HISC. The fractured chain was examined in terms of its microstructure, hardness, transition temperature and strength. An attempt was made to explain why the fractured chain failed in-service.The tensile test of the fractured chain revealed that its yield strength was 140MPa lower than that of the sandblasted chain. It was considered possible that the fracture chain were weaker than they should have been and that the reason it failed in-service was due to overload.The susceptibility of the two chains to HISC was tested by submerging tensile specimens into a 3.5% NaCl solution in CorTest Proof rings, while exposed to hydrogen. Specimens were either hot dip galvanized (HDG), protected by an external potential of -1050mV vs. Ag/AgCl or freely exposed at their corrosion potential. Some specimens were pulled in steps till fracture, while others were held at a constant load for 14 days.Different loading and corrosion potentials were applied to the two chain qualities during the HISC test, but the experiment was unable to provoke brittle fractures in either of them. No cracking was observed in the SEMivfor any of the HDG tensile specimens. It was found that hydrogen have no influence the mechanical properties of the HDG tensile specimens, while hydrogen appear to assist in the development of cracks on the polarized specimens.
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Furuhashi, Toyohisa. "STUDY OF PROPANE ADSORPTION ISOTHERM ON PURIFIED HIPCO SINGLE-WALLED CARBON NANOTUBES." Available to subscribers only, 2009. http://proquest.umi.com/pqdweb?did=1967978731&sid=2&Fmt=2&clientId=1509&RQT=309&VName=PQD.
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Esenwein, Philipp Maximilian. "Histo-morphologische und biochemische Evaluierung der neuen indirekten Revaskularisationsmethode Transmyokardiale Laserrevaskularisation (TMLR)." [S.l.] : [s.n.], 1999. http://deposit.ddb.de/cgi-bin/dokserv?idn=960984429.
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Geffroy, Marie-Christine. "Morphometrie, histo et cytodifferenciation au cours de l'organogenese de la prostate humaine." Paris 5, 1992. http://www.theses.fr/1992PA05S005.
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Trois cent foetus et enfants ages de 8 semaines post-conceptionnelles jusqu'a 1 an ont ete etudies. Les methodes d'etudes appliquees ont ete la morphometrie informatisee et par comptage de points, la reconstruction selon la methode de born, les techniques histologiques standards, et des techniques histochimiques et immunohistochimiques. La morphometrie a montre une courbe bimodale, illustrant l'action des differents facteurs de croissance, des androgenes testiculaires et des oestrogenes maternels et placentaires. L'etude de la disposition des bourgeons en histologie standard et sur les reconstructions conforte la description en trois zones (centrale, peripherique et zone de transition) de l'anatomie prostatique. La cytodifferenciation specifique et non specifique a ete analysee aussi bien pour le mesenchyme prostatique que pour le contingent epithelial. Cette analyse a permis de montrer l'existence de gradients de differenciation entre les differents antigenes (actine et desmine, keratines, pap et psa), comme pour un antigene donne au cours de l'organogenese. Les memes gradients de differenciation ont ete retrouves pour l'expression des mucines. Les malformations de la prostate ont ete etudiees sur 63 foetus et enfants. Diverses alterations de l'architecture et de la cytodifferenciation epitheliale specifique ont pu etre retrouvees au cours de plusieurs syndromes malformatifs, chromosomiques et non chromosomiques. Une seule malformation primitive de la prostate a ete retenue, le syndrome de prune belly. Enfin, certains aspects retrouves au cours de la periode foetale ont ete rapproches d'aspects particuliers histologiques et histopathologiques de l'adulte, et notamment l'expression constante de l'antigene specifique de prostate par les canaux excreteurs des glandes de cowper et de littre, aussi bien de par l'epithelium des glandes prostatiques.
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Daher, Meraneh Abdourahman. "Détermination du sexe chez le palmier dattier : approches histo-cytologiques et moléculaires." Thesis, Montpellier 2, 2010. http://www.theses.fr/2010MON20069.
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Le palmier dattier (Phoenix dactylifera L.) est une espèce fruitière dioïque tropicale qui revêt une importance capitale sur le plan alimentaire, socio-économique et écologique pour les régions arides du globe. Malgré l'intérêt de disposer d'un outil moléculaire pour discriminer les plante s mâles et femelles pour les programmes d'amélioration génétique, aucun marqueur spécifique du sexe n'a été identifié et validé à ce jour. Afin de pouvoir étudier et comprendre le déterminisme sexuel du palmier dattier, nous avons entrepris la description et la caractérisation des processus cellulaires et moléculaires associés à la différenciation des organes sexuels. L'étude histologique du développement reproducteur a montré que le bourgeon floral est d'apparence bisexuelle jusqu'à l'initiation des primordia de l'androcée et du gynécée. Le premier dimorphisme sexuel observé à ce stade correspondant à un gynécée plus large dans les fleurs femelles résulterait d'une activité mitotique plus importante dans les cellules du gynécée fertile par rapport à son équivalent non fonctionnel. Les organes sexuels stériles, staminodes et pistillodes, cessent ensuite leur développement et présentent une différentiation incomplète. Des études d'hybridation in situ de l'expression du gène codant l'histone H4, marqueur de l'activité mitotique, ont montré que le blocage du développement des staminodes et des pistillodes serait dû à un arrêt des divisions cellulaires. Nos investigations de l'intégrité cellulaire par des observations en microscopie électronique à transmission et par coloration de l'ADN confirmeraient que l'avortement des organes stériles ne résulte pas d'un processus de dégradation cellulaire et nucléaire. De plus, l'étude de la méthylation de l'ADN par immunodétection des cytosines méthylées révèle que, par rapport aux organes fertiles, les pistillodes et les staminodes se distinguent par leur niveau plus élevé de méthylation. Ces résultats sont en cohérence avec la réversibilité du blocage de ces organes observés in planta ou in vitro en réponse à une induction hormonale. L'ensemble de ces données montrent que l'unisexualisation des fleurs de palmier dattier est associée à une hyperméthylation globale de l'ADN suivi d'un arrêt des divisions cellulaires dans les organes sexuels stériles. Cette étude a permis d'améliorer nos connaissances sur les mécanismes qui gouvernent la différenciation des organes sexuels et permettra d'ouvrir des perspectives pour l'identification de marqueurs moléculaires du sexe chez le palmier dattierThe date palm (Phoenix dactylifera L.) is a dioecious tropical fruit crop plant which has vital dietary, socio-economic and ecological importance in arid regions of the world. Despite the interest of developing molecular tools to discriminate male and female plants for the benefit of biodiversity preservation and genetic improvement programs, no sex-specific markers have been identified and validated to date. To study and understand the sex determination of date palm, we undertook to characterise the cellular and molecular processes underlying sex organ differentiation in this plant.A histological study of date palm reproductive development showed that the immature flower is bisexual in appearance until the initiation of the androecium and gynoecium. The first sign of sexual dimorphism is observed at this stage, namely a wider gynoecium in female flowers resulting from greater mitotic activity in the functional gynoecium of female flowers compared to the pistillode of male ones. The sterile sex organs (pistillode and staminodes) were observed to cease their development by progressive loss of cell proliferation and ultimately displayed incomplete differentiation.Cell division patterns and the nuclear integrity of reproductive organs were investigated respectively by RNA in situ hybridization to a histone H4 gene probe and by DNA coloration combined with scanning electron microscopy. The results obtained revealed an absence of cell cycle activity and nuclear degradation in the residual sex organs. In addition, a study of DNA methylation, by immunodetection of methylated cytosines revealed that compared to the fertile reproductive organs, staminodes and pistillodes displayed relatively high levels of global DNA methylation. These results are consistent with the observed reversibility of sterile organ developmental arrest observed in planta or in vitro in response to hormonal induction. Overall, these data demonstrate that the floral unisexuality of date palm is characterized by cell cycle arrest, higher DNA methylation in sterile sexual organs and an absence of cell degeneration rather than a cell death process. This study has improved our understanding of the mechanisms that govern the differentiation of sex organs and forms a useful starting point for research on the identification of molecular markers of sex determination in date palm.Kewords: Date palm - flower - sex determination - cell cycle - DNA methylation
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Ecton, Philip. "Low-Energy Electron Irradiation of Preheated and Gas-Exposed Single-Wall Carbon Nanotubes." Thesis, University of North Texas, 2016. https://digital.library.unt.edu/ark:/67531/metadc955114/.
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We investigate the conditions under which electron irradiation of single-walled carbon nanotube (SWCNT) bundles with 2 keV electrons produces an increase in the Raman D peak. We find that an increase in the D peak does not occur when SWCNTs are preheated in situ at 600 C for 1 h in ultrahigh vacuum (UHV) before irradiation is performed. Exposing SWCNTs to air or other gases after preheating in UHV and before irradiation results in an increase in the D peak. Small diameter SWCNTs that are not preheated or preheated and exposed to air show a significant increase in the D and G bands after irradiation. X-ray photoelectron spectroscopy shows no chemical shifts in the C1s peak of SWCNTs that have been irradiated versus SWCNTs that have not been irradiated, suggesting that the increase in the D peak is not due to chemisorption of adsorbates on the nanotubes.
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LEMEE, MAURY PASCALE. "Prevention des intolerances endometriales au sterilet : une nouvelle approche de l'examen histo-pathologique." Toulouse 3, 1994. http://www.theses.fr/1994TOU31012.
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Maur, Christoph auf der Hodel Markus. "Age dependency of ABO histo-blood group antibodies: reexamination of an old dogma /." [S.l.] : [s.n.], 1993. http://www.ub.unibe.ch/content/bibliotheken_sammlungen/sondersammlungen/dissen_bestellformular/index_ger.html.
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Tomica, Svea Isabel. "Umweltresistenz und histo-morphologisches Korrelat der Zwischenleisten, eine weitgehend unbekannte Struktur der menschlichen Leistenhaut." [S.l.] : [s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=971934339.
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Chodnevskaja, Irina. "Histo-morphologische Untersuchungen nach xenogener Transplantation mikroverkapselter Langerhans-Inseln im Modell "Schwein-auf-Ratte"." Diss., lmu, 2006. http://nbn-resolving.de/urn:nbn:de:bvb:19-58123.
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Tempest, N. "Adult epithelial stem cells in the human endometrium : existence, location and 3D histo-architecture." Thesis, University of Liverpool, 2018. http://livrepository.liverpool.ac.uk/3026392/.
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Deluche, Mouricout Elise. "Implication des biomarqueurs NTRK2 et CHI3L1 dans la nouvelle classification histo-moléculaire des gliomes." Thesis, Limoges, 2018. http://www.theses.fr/2018LIMO0063/document.
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Les gliomes, tumeurs cérébrales primaires du système nerveux central, sont souvent de pronostic défavorable, d'autant plus que l'absence de critères indiscutables pour les identifier rend leur diagnostic et leur prise en charge particulièrement difficiles. L’analyse conjointe, d’une cohorte française de 64 patients porteurs de gliomes et d’une cohorte internationale de 671 patients issus du TCGA, a permis de mettre en évidence deux groupes pronostiques constitués par un panel d’expression différentielle de 26 gènes (p = 0,007). Cette stratification en deux groupes pronostiques a été confirmée quels que soient le grade et le groupe moléculaire de la tumeur (p < 0,0001). Nous avons établi une nouvelle stratégie diagnostique à partir de la classification moléculaire des gliomes en intégrant deux biomarqueurs pronostiques CHI3L1 et NTRK2. L’analyse multivariée confirme que ces biomarqueurs sont indépendants du statut IDH et du grade tumoral. Si nous avons mis en évidence par l’analyse protéique de CHI3L1 une concordance avec les transcrits, les résultats divergent pour TrkB. Ainsi, une expression élevée de TrkB et son corécepteur p75NTR serait liée à l’agressivité tumorale indépendamment du statut IDH. Enfin, TrkB et p75NTR sont présents aussi bien dans les exosomes issus du plasma de témoins sains et de patients atteints de gliomes mais leur expression augmente en fonction de l’agressivité de la tumeurGliomas, primary brain tumours of the central nervous system, are often of poor prognosis.The absence of clear criteria to identify them makes their diagnosis and management particularly difficult. The combined analysis of a cohort of 64 glioma patients and an international cohort of 671 patients from the TCGA revealed two prognostic groups of a differential expression panel of 26 genes (p = 0.007). This stratification into two prognostic groups was confirmed independently of the grade and molecular group of the tumor (p <0.0001). We have established a new diagnostic strategy based on the molecular classification of gliomas by integrating two prognostic biomarkers CHI3L1 and NTRK2. Multivariate analysis confirms that these biomarkers are independent of IDH status and tumor grade.While we have demonstrated by the protein analysis of CHI3L1 concordance with the transcripts, the results are different for TrkB. Therefore, a high expression of TrkB and its p75NTR co-receptor would be associated with tumor aggressiveness regardless of IDH status. Lastly, TrkB and p75NTR are present in exosomes from plasma of healthy controls and glioma patients, but their expression increases with the aggressiveness of tumor
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Barreira, Yara de Araujo. "L'étude histologique, histochimique, histo-enzymologique, et morphométrique des vésicules séminales chez le veau sous anabolisant." Toulouse, INPT, 1993. http://www.theses.fr/1993INPT004A.
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Le but de ce travail est de determiner les effets des associations estradiol-testosterone et estradiol-acetate de trenbolone sur les vesicules seminales chez le veau. Les modifications histologiques, histochimiques, histoenzymologiques et morphometriques ont ete etudiees sur des veaux traites par comparaison avec les veaux temoins. Le principal resultat de cette etude est l'augmentation de la surface des acini et de l'epithelium glandulaire chez les veaux sous anabolisants
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Camargo, Ulisses. "Sistemas Histo-sanguíneos ABO, Secretor e Lewis como fatores de risco para a espondilite anquilosante." Faculdade de Medicina de São José do Rio Preto, 2016. http://hdl.handle.net/tede/396.
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Introduction. The spondyloarthritis encomprises a group of diseases strongly associated with HLA-B*27 gene. It has been proposed that genes not belonging to the major histocompatibility complex human influence the genesis of these diseases especially in patients HLA-B*27 negative. Objectives. The aim of this study was to test the hypothesis that the antigens of the ABO, Secretor and Lewis histo-blood systems are associated with spondyloarthritis, especially ankylosing spondylitis (AS). Material and methods. Three hundred and ninety-four patients with clinical suspicion of spondyloarthritis sent for identification of HLA-B*27 gene were analyzed. One hundred and nineteen (30.2%) had confirmed the diagnosis of spondyloarthritis according to the ASAS criteria. The remaining 275 (69.8%) were used as controls. The identification of HLA-B*27 gene was performed using the PCR-SSOP method. The identification of the antigens of the ABO, Secretor and Lewis histo-blood systems was performed using hemagglutination and PCR-RFLP methods. The exact Fisher's test, the chi-square, and the values of Odds Ratio (OR) and Confidence Interval set at 95% were calculated using the GraphPad INSTAT software, accepting the error of 5%. Results. No statistically significant differences were observed in the frequency of antigenic profiles of ABO (χ2: 1.152; p = 0.764; GL: 3), Secreto (χ2: 0.779; p = 0.377; GL: 1) and Lewis (χ2: 1.853; p = 0.396; GL: 2) histo-blood groups between patients and controls. The Lea antigen was more frequent in patients with AS compared to controls (OR: 1.833; 95% CI: 1025- 3284, p = 0.053). This antigen was strongly associated with AS in HLA-B*27 negative patients compared to controls (OR: 4.469; 95% CI: 1931-10342; p = 0.0007). This association remained only in males in the absence of HLA-B*27 gene (OR: 6.880; 95% CI: 1852-25564; p = 0.004). Conclusions. AS is associated to the Lea antigen in HLAB* 27 negative male patients.
Introdução. As espondiloartrites compreendem um grupo de doenças fortemente associadas ao gene HLA-B*27. Tem sido proposto que genes não pertencentes ao complexo principal de histocompatibilidade humano influenciam a gênese destas doenças especialmente nos pacientes HLA-B*27 negativos. Objetivos. O objetivo deste estudo foi testar a hipótese de que os antígenos dos sistemas histo-sanguíneos ABO, Secretor e Lewis estão associados à espondiloartrites, especialmente a espondilite anquilosante (EA). Material e método. Foram analisados 394 pacientes com suspeita clínica de espondiloartrites encaminhados para identificação do gene HLA-B*27. Cento e dezenove (30,2%) tiveram o diagnóstico de espondiloartrite confirmado de acordo com os critérios ASAS. Os 275 (69,8%) restantes compuseram o grupo controle. A identificação do gene HLA-B*27 foi realizada com o uso do método PCR-SSOP. A caracterização dos antígenos dos sistemas histo-sanguíneos ABO, Secretor e Lewis foi realizada com o uso dos métodos hemaglutinação e PCR-RFLP. O teste exato de Fisher, o qui-quadrado, os valores de Odds Ratio (OR) e do intervalo de confiança a 95% foram calculados com o uso do software GraphPad Instat, aceitando o erro de 5%. Resultados. Não foram observadas diferenças estatisticamente significantes nas frequências dos perfis antigênicos dos sistemas histo-sanguíneos ABO (χ2: 1.152; p=0,764; GL: 3), Secretor (χ2: 0.779; p=0,377; GL: 1) e Lewis (χ2: 1.853; p=0,396; GL: 2) de pacientes e controles. Foi observada maior frequência do antígeno Lea em pacientes com EA, comparados aos controles (OR: 1.833; IC 95%: 1.025 – 3.284; p=0,053). Este antígeno mostrou-se fortemente associado à EA em pacientes HLA-B*27 negativos comparados aos controles (OR: 4.469; IC 95%: 1.931 – 10.342; p=0,0007). Esta associação se manteve apenas no gênero masculino na ausência do gene HLA-B*27 (OR: 6.880; IC 95%: 1.852 – 25.564; p = 0,004). Conclusões. A EA está associada ao antígeno Lea nos pacientes masculinos HLA-B*27 negativos.
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Sharifi, Ghazvini-Salamatian Vénus. "Approche statistique de l'hétérogénéité intratumorale et application aux grades histo-pathologiques du cancer du sein." Paris 7, 2002. http://www.theses.fr/2002PA077175.
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Courtois, Ivan. "Prothèses mammaires et rétraction capsulaire : approche analytique, bases histo-mécaniques d'un nouveau type de prothèse." Bordeaux 2, 1989. http://www.theses.fr/1989BOR23075.
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Rossouw, Esmari. "The molecular epidemiology and diversity of gastroenteritis viruses in HIV-infected, -exposed and -unexposed children under the age of five years in Pretoria, South Africa." Diss., University of Pretoria, 2020. http://hdl.handle.net/2263/75468.
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Viruses are common causes of both endemic and epidemic gastroenteritis, infecting millions of people per year, with norovirus, rotavirus and adenovirus-F as the main causative agents, and sapovirus and astrovirus as contributing viruses. These viruses are highly infectious and most severe in the very young, old, or individuals who are immunocompromised. The viral infection usually causes self-limited gastroenteritis, although chronic infection has been observed in highly immunocompromised patients. African and South-East Asian regions are disproportionally affected by diarrhoeal disease. These regions (especially South Africa) are also more severely affected by human immunodeficiency virus (HIV) infections. It has been suggested that immunocompromised individuals may form part of a reservoir for novel virus variants and recombinants.
It should be taken into account that not every person is equally susceptible to infection after pathogen exposure and that not all infected persons develop clinical symptoms (Ramani and Giri, 2019). One host genetic factor that can influence susceptibility to enteric infection is the expression of histo-blood group antigens (HBGAs). Histo-blood group antigens are a major group of complex carbohydrates and are determinants of both human and animal ABO blood groups and the Lewis blood group systems, which are distributed in abundance on the mucosal epithelia of the gastrointestinal tract. Histo-blood group antigens have been proven to influence susceptibility to rotavirus and norovirus infections.
Saliva, blood and stool specimens (n=205) have previously been collected from children (≤ 5 years of age) hospitalised with gastroenteritis at Kalafong Provincial Tertiary Hospital from June 2016 to December 2017. Follow up stool specimens were then collected six weeks after enrolment when possible. A descriptive questionnaire was completed by each child’s guardian, giving information on age, residential area, HIV status etc. of the participating child. The stool specimens were screened for six gastroenteritis causing viruses (norovirus GI and –GII, rotavirus, sapovirus, astrovirus and adenovirus) by multiplex PCR. Forty-seven percent (96/205) of specimens tested positive for at least one gastroenteritis causing virus. Rotavirus predominated (46/205), followed by norovirus (32/205), adenovirus (15/205), sapovirus (9/205) and astrovirus (3/205). A total of 27/32 norovirus (GI.3, GII.2, GII.3, GII.4, GII.7, GII.12 and GII.21), 44/46 rotavirus (G1P[8], G2P[4], G2P[6], G3P[4], G3P[8], G8P[4], G8P[6], G9P[6] and G9P[8]) and 8/9 sapovirus (GI.1, GI.2, GII.1, GII.4 and GII.8) strains have been genotyped, of which norovirus GII.4 and rotavirus G3P[4] predominated. A total of 46/205 children submitted a follow up stool specimen to be tested. Of the 46 children, 9 tested positive for norovirus infection with initial stool specimen testing. Follow up screening resulted in 13/46 (28%) specimens testing positive for either norovirus GI or GII, with all patients presenting as asymptomatic. After genotyping it was observed that only one of the follow up specimens were identical to the original sequence genotyped, indicating prolonged shedding. FUT2 genotyping of 205/205 children showed a 71%:29% ratio between secretors and non-secretors. Eighty percent (77/96) of the virus-infected children were secretors whereas only 20% (19/96) were non-secretors. Rotavirus (p<0.01) and norovirus GII.4 (p<0.05) specifically were found to be more prevalent in secretors. In this study, no statistical significance was observed in terms of severity of and susceptibility to gastroenteritis viruses between HIV-infected, HIV-exposed uninfected or HIV-uninfected individuals. Histo-blood group phenotyping has resulted in various combinations, with Le(b) being the most prevalent antigen found.
Next generation sequencing was unsuccessful. In future, fresh specimens should be considered for testing, with more funding and time for optimisation of this process and to give adequate results.
In summary, gastroenteritis is still a leading cause of childhood morbidity and mortality, with all advancements in understanding the disease helping to decrease the impact of it. This study again reinforced the importance of these viruses, as they are circulating in such high abundance. It also reinforced the concept that susceptibility to noro- and rotavirus infection is affected by the secretor status of a person. This could in future help with better understanding the viral infection mechanisms and in turn help with vaccine development and treatmentDissertation (MSc (Medical Virology))--University of Pretoria, 2020.
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Zöllner, Anne. "Untersuchungen zur Amyloidose bei Zoo- und Wildvögeln sowie zur histo- und immunhistochemischen Charakterisierung des aviären Amyloidproteins." [S.l.] : [s.n.], 1997. http://www.diss.fu-berlin.de/1998/103/index.html.
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Aber, Mohamed. "Contribution a l'etude histo-cytologique, physiologique et agronomique du parasitisme chez une orobanchacee : orobanche crenata forsk." Paris 6, 1988. http://www.theses.fr/1988PA066002.
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La germination des graines du parasite est sous la dependance des exsudats racinaires des legumineuses. Apres fixation, le parasite edifie deux foyers organiques: l'un interne, l'haustorium, l'autre externe, le tubercule. L'ontogenese de l'haustorium comprend une premiere phase meristematique suivie d'une differenciation cellulaire. Contrairement aux xylemes, les phloemes demeurent separes par des cellules du parasite. L'initiation des meristemes racinaires et caulinaire, au niveau du tubercule est precisee. Pour lutter contre ce fleau au maroc dans les cultures de legumineuses, on etudie l'action du glyphosate
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Aber, Mohamed. "Contribution à l'étude histo-cytologique, physiologique et agronomique du parasitisme chez une orobanchacée, Orobanche crenata Forsk." Grenoble 2 : ANRT, 1988. http://catalogue.bnf.fr/ark:/12148/cb37611116g.
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Cline, Michael T. Jr. "Analysis of Coincident HICO and Airborne Hyperspectral Images Over Lake Erie Western Basin HABs." University of Toledo / OhioLINK, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1470324418.
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Cheetham, Sonia Maria. "Pathogenesis of human norovirus in gnotobiotic pigs." Columbus, Ohio : Ohio State University, 2006. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1149018306.
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Hu, Jin-Jia. "Pressure-induced growth and remodeling of arteries in a porcine aortic coarctation model." Texas A&M University, 2005. http://hdl.handle.net/1969.1/4982.
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Hypertension is a risk factor for many cardiovascular and cerebrovascular
diseases such as atherosclerosis and stroke. It is therefore important to understand the
effect of hypertension on temporal growth and remodeling of arteries. In this study,
experimental hypertension was induced in the mini-pig by aortic coarctation. Basilar
arteries and aortas were collected for analysis over an eight week period of hypertension
with specimens from normotensive animals serving as controls. Changes in mechanical
properties of the basilar artery were evaluated by in vitro pressure-diameter tests on
intact cylindrical segments at their in situ length. The basilar arteries from hypertensive
animals became less distensible, reflecting increases in both structural and material
stiffness, compared to their normotensive counterparts. The circumferential stress
rapidly returned toward its homeostatic value by increasing the wall thickness within
two weeks. Immunohistochemistry, which is capable of illustrating the localization and
distribution of protein expression, was performed to examine changes in wall
constituents in the aorta. The increased medial thickness observed in hypertensive pigs
compared to normotensive pigs was due to hyperplasia of smooth muscle cells (SMCs)
and accumulation of extracellular matrix proteins, which were accompanied by the phenotypic modulation of SMCs. The increased interlamellar thickness, collagen fibers,
and the thickness of elastic lamina found in the inner media of hypertensive animal may
be associated with the gradient of stress decreasing into the outer media. SMC
proliferation, if any, was found evenly distributed across the media, however. In cases
showing increased proliferation and matrix protein synthesis, the SMC contractile
markers were down-regulated whereas the SMC synthetic markers were up-regulated.
While the aortic intima appeared normal in the normotensive animals, neointima
formation, which may predispose the vessel to atheroma formation, was found in the
hypertensive animals. Immunohistochemistry of Hsp47 and procollagen revealed that
the endothelial cells (ECs) may produce collagen, specifically type I collagen in
response to hypertension and contribute to the thickened intima. In addition, lectin
staining for ECs markers and immunostaining for eNOS suggested that endothelial cells
may transdifferentiate into intimal SMCs. These findings suggested an alternative role
that ECs may play in hypertension-induced atherogenesis.
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Gucia, Julitta [Verfasser], Günter [Akademischer Betreuer] Fürst, and Stephan E. [Akademischer Betreuer] Baldus. "CT-gesteuerte Tumorpunktionen - Histo- und Zytopathologische Auswertung von Gewebeproben / Julitta Gucia. Gutachter: Günter Fürst ; Stephan E. Baldus." Düsseldorf : Universitäts- und Landesbibliothek der Heinrich-Heine-Universität Düsseldorf, 2012. http://d-nb.info/1022885510/34.
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Ikefuti, Cynthia Venâncio. "Avaliação ecotoxicológica, bioquímica, histo-hematológica e eficácia de terapêuticos no controle de doenças em Pacu, Piaractus mesopotamicus /." Jaboticabal, 2016. http://hdl.handle.net/11449/143038.
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Orientador: Maria José Tavares Ranzani PaivaCoorientador: Claudinei da Cruz
Banca: Márcia Rita Fernandes Machado
Banca: Maria Isabel Mataqueiro
Banca: Gilson Pereira de Oliveira
Banca: Eduardo Makoto Onaka
Resumo: Objetivou-se com este estudo foram estimar os valores de concentração letal e efetiva (CE50 e CL50) do imidacloprid (IMD) e da associação de sulfadimetoxina e metronidazol (SM) para oito organismos aquáticos não alvos; avaliar o efeito histopatológico da exposição aguda do IMD e da SM em brânquias, fígado e rins de pacu, Piaractus mesopotamicus. Ajustar a dose, verificar a eficácia, a recuperação (14 dias) do IMD e da SM de pacus infestados/infectados experimentalmente com Anacanthorus penilabiatus e Aeromonas hydrophila pelo monitoramento das variáveis físicas e químicas da água e das alterações histopatológicas, hematológicas, bioquímicas e imunológicas. O valor de CE e CL50 para todos os organismos foi >100,0 mg L-1 e as drogas foram classificadas como praticamente não tóxicas para as oito espécies. Após cinco dias de tratamento com o IMD ocorreu redução significativa de 81,32% no número de parasitos e aumento do número de eritrócitos nos peixes tratados. Após 14 dias do final do tratamento, foi observada redução dos valores de hemoglobina e CHCM dos peixes expostos ao IMD. Com a SM ocorreu crescimento de UFCs somente nas amostras reisoladas dos peixes sadios e infectados não tratados. Também foi observada redução do número de eosinófilos de todos os tratamentos em relação ao controle e aumento dos íons Na+ nos peixes infectados e tratamentos com SM. Após 14 dias ocorreu redução do burst respiratório dos peixes de todos os tratamentos em relação ao controle sadio. As alterações histopatológicas observadas neste estudo foram reversíveis. Os dois fámarcos possuem potencial para registro na aquicultura brasileira, pois atenderam requisitos importantes como baixa toxicidade para organismos não alvos; redução significativa no número de monogenéticos A. penilabiatus e inibição do crescimento da bactéria A. hydrophila presentes em pacus, causando alterações reversíveis em...
Abstract: The aims of this research were to estimate the effective and lethal concentration (CE50 and CL50) of imidacloprid (IMD) and of the association between sulfadimethoxin and metronidazole (SM), on eight non-target aquatic organisms; to evaluate the hystopathological effect of the acute exposure to IMD and SM in the gills, liver and kidneys from pacu fish, Piaractus mesopotamicus; to adjust the dose, verify the efficacy of IMD and SM exposure, and to assess the 14 days recovery of experimentally infested pacu fish with Anacanthorus penilabiatus and Aeromonas hydrophila, through the monitoring of the physical and chemical water variables and the hystopathological, hematological, biochemical and immunological response of the fish. The CE and CL50 was above 100.0 mg L-1 for al tested organisms, and the drugs were classified as practically non toxic. After five treatment days with IMD, a significant decrease in parasite count (81.32%) and the increase in erythrocyte number were observed. A reduction in hemoglobin and CHCM count was observed 14 days after the treatment, in the fish exposed to IMD. The SM exposure promoted UFC growth only in the reisolated samples from the healthy and in the non-treated infected fish. The reduction in eosinophils count occurred in all treatments, in comparison to the control. The exposure to SM promoted the increase of Na+ ions in the infected fish. After 14 days, the reduction of burst was observed in all treatments in comparison to the healthy control. All the hystopathological responses observed in the present research were reversible. Both drugs displayed registry potential in Brazilian aquaculture, for reaching the key requirements, such as low toxicity in non-target organisms; significant reduction in the monogenean A. penilabiatus; growth inhibition of the bacteria A. hydrophila in P. mesopotamicus; and promoted reversible changes in the fish
Doutor
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Ikefuti, Cynthia Venâncio [UNESP]. "Avaliação ecotoxicológica, bioquímica, histo-hematológica e eficácia de terapêuticos no controle de doenças em Pacu, Piaractus mesopotamicus." Universidade Estadual Paulista (UNESP), 2016. http://hdl.handle.net/11449/143038.
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000866922.pdf: 1979894 bytes, checksum: 803f275069fd703cdd89ee088eaf3280 (MD5)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Objetivou-se com este estudo foram estimar os valores de concentração letal e efetiva (CE50 e CL50) do imidacloprid (IMD) e da associação de sulfadimetoxina e metronidazol (SM) para oito organismos aquáticos não alvos; avaliar o efeito histopatológico da exposição aguda do IMD e da SM em brânquias, fígado e rins de pacu, Piaractus mesopotamicus. Ajustar a dose, verificar a eficácia, a recuperação (14 dias) do IMD e da SM de pacus infestados/infectados experimentalmente com Anacanthorus penilabiatus e Aeromonas hydrophila pelo monitoramento das variáveis físicas e químicas da água e das alterações histopatológicas, hematológicas, bioquímicas e imunológicas. O valor de CE e CL50 para todos os organismos foi >100,0 mg L-1 e as drogas foram classificadas como praticamente não tóxicas para as oito espécies. Após cinco dias de tratamento com o IMD ocorreu redução significativa de 81,32% no número de parasitos e aumento do número de eritrócitos nos peixes tratados. Após 14 dias do final do tratamento, foi observada redução dos valores de hemoglobina e CHCM dos peixes expostos ao IMD. Com a SM ocorreu crescimento de UFCs somente nas amostras reisoladas dos peixes sadios e infectados não tratados. Também foi observada redução do número de eosinófilos de todos os tratamentos em relação ao controle e aumento dos íons Na+ nos peixes infectados e tratamentos com SM. Após 14 dias ocorreu redução do burst respiratório dos peixes de todos os tratamentos em relação ao controle sadio. As alterações histopatológicas observadas neste estudo foram reversíveis. Os dois fámarcos possuem potencial para registro na aquicultura brasileira, pois atenderam requisitos importantes como baixa toxicidade para organismos não alvos; redução significativa no número de monogenéticos A. penilabiatus e inibição do crescimento da bactéria A. hydrophila presentes em pacus, causando alterações reversíveis em...
The aims of this research were to estimate the effective and lethal concentration (CE50 and CL50) of imidacloprid (IMD) and of the association between sulfadimethoxin and metronidazole (SM), on eight non-target aquatic organisms; to evaluate the hystopathological effect of the acute exposure to IMD and SM in the gills, liver and kidneys from pacu fish, Piaractus mesopotamicus; to adjust the dose, verify the efficacy of IMD and SM exposure, and to assess the 14 days recovery of experimentally infested pacu fish with Anacanthorus penilabiatus and Aeromonas hydrophila, through the monitoring of the physical and chemical water variables and the hystopathological, hematological, biochemical and immunological response of the fish. The CE and CL50 was above 100.0 mg L-1 for al tested organisms, and the drugs were classified as practically non toxic. After five treatment days with IMD, a significant decrease in parasite count (81.32%) and the increase in erythrocyte number were observed. A reduction in hemoglobin and CHCM count was observed 14 days after the treatment, in the fish exposed to IMD. The SM exposure promoted UFC growth only in the reisolated samples from the healthy and in the non-treated infected fish. The reduction in eosinophils count occurred in all treatments, in comparison to the control. The exposure to SM promoted the increase of Na+ ions in the infected fish. After 14 days, the reduction of burst was observed in all treatments in comparison to the healthy control. All the hystopathological responses observed in the present research were reversible. Both drugs displayed registry potential in Brazilian aquaculture, for reaching the key requirements, such as low toxicity in non-target organisms; significant reduction in the monogenean A. penilabiatus; growth inhibition of the bacteria A. hydrophila in P. mesopotamicus; and promoted reversible changes in the fish
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Jankauskas, Antanas. "Visiška kairiosios Hiso pluošto kojytės blokada ir vainikinių arterijų angiografijos daugiapjūviu kompiuteriniu tomografu tyrimo vertė." Doctoral thesis, Lithuanian Academic Libraries Network (LABT), 2010. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2009~D_20100422_112031-11928.
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Vainikinių arterijų tyrimas daugiapjūviu kompiuteriniu tomografu (DKT) į klinikinę praktiką įdiegtas nesenai. Tai neinvazyvus tyrimo metodas, kurį galima atlikti ir ambulatoriniams pacientams. Tačiau greta savo privulumų, jis turi ir trūkumų – dėl nuolatinio vainikinių arterijų judėjimo širdies ciklo metu gali atsirasti liekamųjų judesio artefaktų atkuriant vaizdus. Jų tikimybė priklauso ne tik nuo aparato, kuriuo atliekamas tyrimas, techninių charakteristikų, bet ir nuo širdies veiklos ypatumų. Yra atlikta nemažai tyrimų, įvertinančių vainikinių arterijų vaizdų kokybės priklausomybę nuo širdies susitraukimų dažnio, jo variabilumo, kitų faktorių. Kairiosios Hiso pluošto kojytės blokados, sąlygojančios nesinchronišką skilvelių susitraukimą ir atsipalaidavimą, įtaka vaizdų kokybei tyrinėta mažai. Šiame tyrime buvo palyginta vaizdų kokybė tarp pacientų, kuriems nustatyta visiška kairiosios Hiso pluošto kojytės blokada ir nėra laidžiosios sistemos sutrikimų, grupių. Analizuota vainikinių arterijų įvertinimo visuose širdies ciklo rekonstrukciniuose intervaluose įtaka vaizdų kokybės vidurkiui. Nustatyta, kokia yra DKT tyrimo diagnostinė vertė identifikuojant hemodinamiškai reikšmingas vainikinių arterijų stenozes. Kadangi DKT tyrimas susijęs su jonizuojančia spinduliuote, įvertinti rentgeno vamzdžio srovės stiprumo moduliavimo ypatumai. Taip pat nustatyta širdies susitraukimų dažnio, jo variabilumo įtaka vaizdų kokybei, esant visiškai kairiosios Hiso pluošto kojytės blokadai.Multislice computed tomography (MSCT) coronary angiography is one of the latest usage area of the computed tomography technology. It s a non-invasive diagnostic tool, wich can be performed in ambulant patients. MSCT coronary angiography beside it's advantages has some drawbacks. The main drawbacks are lower spatial resolution and residual motion artifacts in MSCT images. Therefore quality of coronary images and diagnostic value of MSCT angiography differs, depending on patient's clinical characteristics, especially on heart contraction features during scanning. Asynchronic contraction and relaxation of the right and left ventricles, caused of bundle branch block can also influence image quality of MSCT coronary angiography. To date, there are only few studies, conducted to analyse diagnostic performance of MSCT coronary angiography in patients with left bundle branch block. Although influence of heart rate, heart rate variability and the other factors (for example, age, sex, body mass index) on MSCT image quality are quite well investigated. Thus, the present study were designed to investigate the influence of complete left bundle branch block on image quality of MSCT coronary angiography more detail in comparison with control patients group without electrical conduction disturbances, reveal the diagnostic value of MSCT angiography to identify hemodinamicaly significant coronary stenosis.
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Vale, Alessandra Pinto Antunes do. "O conto de Apepi e Sequenenra (Reino Novo, XIXª Dinastia): uma análise histórico-literária." reponame:Repositório Institucional da UFF, 2013. https://appdesenv.uff.br/riuff/handle/1/211.
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A contenda de Apepi e Sequenenra encontra-se preservada em uma única versão: o Papiro Sallier I. Esse documento foi redigido durante a XIXª dinastia, tendo sido escrito no período de reinado do faraó Merenptah (c. 1213-1203 a.C.). Apesar de escrito no Reino Novo, o Conto de Apepi e Sequenenra aborda, ficcionalmente, um episódio de meados do século XVI a.C., envolvendo personagens históricos do final do Segundo Período Intermediário: o faraó hicso Apepi, da XVª dinastia, e o rei Sequenenra, da XVIIª dinastia tebana. Através dele é possível levantar alguns interessantes questionamentos, dentre os quais dois se destacam: (1) a disputa entre governantes – Apepi, hicso, e Sequenenra, egípcio; (2) e a oposição entre deuses, nesse caso especificamente Amon-Ra e Seth (Sutekh, para os hicsos), paralela à dos reis que lhes prestavam culto monolátrico
Lê conte de Apepi Sequenenra est conserve dans um seule version: lê Papyrus Sallier I. Ce document a été redige au cours de la Dix-Neuvième Dynastie, ayant été écrit durant lê règne de la pharaon Merenptah (c. 1213- avant J.C.). Bien que rédigé dans la Nouvel Empire, lê Conte de Apepi et Sequenenra, parle fictivement de um épisode de milieu du XVIe siècle, impliquant dês personnages historiques de la fin de la Dèuxieme Période Intermédiaire: lê pharaon hyksos Apepi, de la quinziéme dynastie, et le roy Sequenenra, de la dix-septième dynastie thébaine. Grace a lui, vous pouvez soulever quelques questions interessantes, dont deux se distinguite: (1), l’ opposition entre lês gouverneurs – Apepi, hyksos, et Sequenenra, égyptien; (2) et l’ opposition entre les dieux, dans ce cãs spécifiquement Amon-Ra et Seth (Sutekh, pour les Hyksos), parallèlement à dês róis qui les adoraient monolâtriement.
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Lecolier, Aurélie. "Caractérisation de certains impacts de la mutation "Laurina" chez "Coffea arabica L. " aux niveaux histo-morphologique et moléculaire." Phd thesis, Université de la Réunion, 2006. http://tel.archives-ouvertes.fr/tel-00468113.
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Le caféier Coffea arabica var. Laurina, aussi appelé Bourbon Pointu, est apparu à la Réunion suite à une mutation spontanée de la variété Bourbon. Cette mutation Laurina, monolocus et récessive, a des effets pléiotropiques qui différencie le Bourbon pointu du Bourbon. Au niveau morphologique, elle se caractérise par un nanisme, un port pyramidal et une forme pointue de ses graines. A un niveau moléculaire, la teneur des grains en caféine est fortement réduite. Malgré des caractéristiques agronomiques d'intérêt et d'excellentes qualités organoleptiques, peu d'études sont disponibles sur ce mutant naturel. Ce travail a ainsi pour objectif d'étudier la mutation Laurina et d'en caractériser les effets afin de mieux décrire les cascades de réaction mises en place. Il se base sur la comparaison du Bourbon pointu avec sa variété parente Bourbon. Au niveau morphologique, des mesures de croissance végétative ont permis l'analyse et l'explication de la forme pyramidale du mutant. Au niveau histologique, l'étude de l'apex, centre initiateur des organes de surface, et de différents entre-noeuds de l'axe orthotrope a permis d'expliquer l'origine du nanisme en terme de division et d'élongation cellulaire. Des hypothèses quant à l'action de la mutation Laurina sur certaines hormones ont été émises à partir des résultats d'application de gibbérelline exogène. Ces études macro et microscopiques ont été couplées au niveau moléculaire à la recherche de gènes différentiellement exprimés entre les deux variétés. La comparaison des transcriptomes des deux variétés à un stade précoce post-cotylédonaire avait pour but la recherche de gènes candidats impliqués dans les premières cascades de réactions menant aux effets pléiotropiques observés. Le clonage différentiel basé sur la méthode SSH (Hybridation Suppressive Soustractive) couplé à une étape de tri à haut débit (macro-array) a été appliqué à ces fins. L'ensemble des résultats décrit plus précisément les effets pléiotropiques induits par la mutation Laurina. La description précise des effets pléiotropiques de la mutation ouvre des pistes quant à la caractérisation moléculaire de la mutation à travers une approche gène candidat.
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Carvalho, Danila Blanco de [UNESP]. "Estudo da associação entre o sistema histo-sangüíneo ABO e a malária por Plasmodium falciparum na Amazônia brasileira." Universidade Estadual Paulista (UNESP), 2008. http://hdl.handle.net/11449/94836.
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carvalho_db_me_sjrp.pdf: 1649413 bytes, checksum: 0ac4714a8b68e2f57418348441007ee7 (MD5)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
O sistema sangüíneo ABO (sABO) é o mais importante sistema na compatibilidade de grupos sangüíneos. Muitas pesquisas têm mostrado associações deste sistema com várias doenças infecciosas, inclusive a malária. Este estudo avaliou a associação entre os genótipos do sistema histo-sangüíneo ABO e a malária não grave causada pelo Plasmodium falciparum. A genotipagem dos grupos sangüíneos do sistema ABO foi feita de acordo com o protocolo de PCR/ RFLP, em amostras de indivíduos maláricos e não maláricos de áreas da Amazônia brasileira. O genótipo homozigoto ABO*O01O01 foi prevalente tanto nos maláricos quanto nos doadores de sangue. O genótipo ABO*AB representou cerca de 3% da população infectada e 5% da não infectada. Não foram verificadas diferenças estatisticamente significantes na comparação das freqüências alélicas e genotípicas do sABO entre pacientes e grupo controle, mesmo quando foram analisados apenas indivíduos com infecções puras de P. falciparum. A freqüência do sABO na Amazônia brasileira pode estar relacionada com a baixa freqüência de malária grave pelo P. falciparum. Portanto, os genótipos encontrados no sistema ABO dos indivíduos maláricos e não maláricos pode promover relevantes informações, para o entendimento da epidemiologia da malária grave por P. falciparum na Amazônia brasileira.
The ABO blood system (sABO) is the most important system on the blood groups compatibility. Several studies have shown its associations with various infectious diseases, including malaria. This study evaluated the association between the ABO histo-blood genotypes and non-severe malaria caused by Plasmodium falciparum. PCR/RFLP protocol had be used for both ABO blood group system genotyping in malaria suffering individuals and blood donors, from malaria areas of the Brazilian Amazon. The homozygous genotype ABO*O01O01 was prevalent in both malaria and the blood donors. The genotype ABO*AB represented about 3% of the infected population and 5% of non-infected. No statistically significant differences were observed in sABO genotypic and allelic frequencies of patients and the control group, even when individuals were analyzed only with pure infection of P. falciparum. The frequency of sABO in the Brazilian Amazon may be related to the low frequency of non-severe malaria P. falciparum. Therefore, the genotypes found in the ABO blood system in malaric and non-malaric individuals can promote relevant information for the understanding of the severe malaria by P. falciparum epidemiology in the Brazilian Amazon. Keywords: Malaria; ABO blood group system; Plasmodium falciparum.
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Martz, Paula Miriam [Verfasser]. "Histo-pathologic alterations of lung tissue caused by hypoxia in neonates deceased due to dystocia / Paula Miriam Martz." Gießen : Universitätsbibliothek, 2017. http://d-nb.info/1148264418/34.
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Bernardo, Cássia Rubia. "Associação entre os sistemas histo-sanguíneos ABO, Secretor e Lewis e as formas clínicas da Doença de Chagas." Faculdade de Medicina de São José do Rio Preto, 2014. http://hdl.handle.net/tede/301.
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Fundação de Amparo à Pesquisa do Estado de São Paulo - FAPESP
Introduction: Chagas disease is caused by the protozoan Trypanosoma cruzi, which is transmitted to humans commonly in the feces of a hemipterous popularly known as barber. The natural infection occurs mainly in childhood. After a period of approximately two decades infected individuals develop clinical manifestations such as Chagas heart disease and Chagas gastrointestinal disease (Megaesophagus and/or Megacolon). The expression of the antigens belonging to histo-blood systems ABO, Secretor and Lewis, controlled by the genes ABO (9q34.1), FUT2 (19q13.3) and FUT3 (19p13.3) differs between the organs affected by Chagas disease. It is possible that the differential tissue expression of ABO, Secretor and Lewis histo-blood groups influences the clinical manifestations of Chagas disease. Aim: The aim if this study was to verify if the antigens of the histo-blood systems ABO, Secretor and Lewis are associated with different clinical forms of Chagas disease. Materials and methods: After obtaining the informed consent peripheral blood and serum samples from 827 individuals were collected. Patients were divided into three subgroups according to their clinical state (megacólon [n=66], megaesophagus [n=119] and cardiomyopathy [n=154]). The control group consisted of 488 blood donors properly fit for the donation. The Lewis and ABO phenotyping were performed by hemagglutination test tube and gel columns agglutination, respectively. The IgG anti-T. cruzi antibodies were identified by ELISA. FUT2 and FUT3 genotyping were carried out by PCR-RFLP. Results: The mean age of patients with Chagas disease was 64.8±11.2 and blood donors 34.3±11.0 (p<0.0001). The differences between the percentages of the sex of the patients and donors were statistically significant (p <0.0001). The frequencies of ABO, Secretor and Lewis distributed in the three forms of the disease compared with each other and with donors, did not give differences statistically significant. The comparison between the ABO and Secretor combined, according to the three forms of Chagas disease, showed statistically significant differences for megaesophagus form (p=0.015). The frequencies of ABO, Secretor and Lewis antigen profiles between patients and donors showed differences statistically significant in favor of BLeb antigen (p=0.032). Conclusion: The results suggest that the high expression of antigen B, which characterizes the B and AB blood groups under the control of functional FUT2 (Secretor) gene acts as a risk factor for megaesophagus form of the Chagas disease.
Introdução: A doença de Chagas é causada pelo protozoário Trypanosoma cruzi, o qual é transmitido ao homem, comumente, pelas fezes de um hemíptero conhecido popularmente como barbeiro. A infecção natural ocorre principalmente na infância e após um período aproximado de duas décadas, parte dos indivíduos infectados desenvolvem manifestações clínicas como a Cardiopatia Chagásica Crônica e a doença do trato gastrointestinal (Megaesôfago e/ou Megacólon). A expressão dos antígenos dos sistemas histo-sanguíneos ABO, Secretor e Lewis, controlada pelos genes ABO (9q34.1), FUT2 (19q13.3) e FUT3 (19p13.3), difere entre os órgãos acometidos por esta doença e pode influenciar suas manifestações clínicas. Objetivo: Avaliar se os antígenos dos sistemas histo-sanguíneos ABO, Secretor e Lewis estão associados às diferentes formas clínicas da Doença de Chagas. Materiais e Métodos: Após a entrevista e obtenção do termo de consentimento livre e esclarecido, amostras de sangue periférico e soro de 827 indivíduos foram analisadas. Os pacientes com a forma crônica da Doença de Chagas foram divididos em três subgrupos de acordo com a forma clínica, (megacólon=66, megaesôfago=119 e cardiomiopatia=154). O grupo controle constitui-se de 488 doadores de sangue devidamente aptos à doação. As fenotipagens ABO e Lewis foram realizadas por métodos de hemaglutinação em tubos e colunas de gel, respectivamente. A pesquisa de anticorpos da classe IgG anti-T. cruzi foi realizada pelo teste de ELISA. Os genótipos FUT2 e FUT3 foram identificados por PCR-RFLP. Resultados: A média de idade dos pacientes chagásicos foi de 64,8±11,2 e dos doadores de sangue 34,3±11,0 (p<0.0001). As diferenças entre as porcentagens do sexo dos pacientes e doadores foram estatisticamente significantes (p< 0.0001). As frequências dos fenótipos ABO, Secretor e Lewis distribuídos nas três formas da doença comparados entre si e com os doadores, não revelaram diferenças estatisticamente significantes. A comparação entre os fenótipos ABO e Secretor combinados, de acordo com as três formas da Doença de Chagas, mostrou diferenças estatisticamente significantes para a forma megaesôfago (p=0,015). A comparação entre as frequências dos perfis antigênicos de pacientes e doadores, revelaram diferença estatisticamente significante para o perfil de antígenos BLeb (p=0,032). Conclusões: Os resultados sugerem que a expressão do antígeno carboidrato B, o qual caracteriza os grupos sanguíneos B e AB, cuja síntese está sob o controle dos genes funcionais FUT2 (Secretor), atua como um fator de risco para a forma megaesôfago da Doença de Chagas.
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Silva, Thelma Regina Gabriel da. "Compostos de reserva em sementes de Dalbergia miscolobium Benth : caracterização histo e bioquimica e variação durante a germinação." [s.n.], 1995. http://repositorio.unicamp.br/jspui/handle/REPOSIP/315586.
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Orientador: Sonia M. C. DietrichTese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia
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Resumo: O presente trabalho, foi realizado com sementes de D. miscolobium, uma espécie arbórea dos cerrados, de importância econômica como madeira e conhecida como cabiuna do cerrado. Como objetivos principais teve: verificar aspectos fisiológicos da germinação das sementes dessa espécie, determinar o padrão de crescimento para o eixo embrionário, detectar as alterações nos conteúdos das principais reservas da semente e sua mobilização e estabelecer parâmetros que permitissem analisar o padrão de desenvolvimento e crescimento da plântula, na fase inicial da germinação. Para atingir esses objetivos foram feitas determinações de massa fresca e massa seca de eixos e cotilédones, caracterizações e detecções citoquímicas e quantificações bioquímicas. Os resultados demonstraram o alto poder germinativo das sementes dessa espécie independentemente da procedência, tamanho ou cor de suas sementes. Entretanto, apesar da padronização das sementes, verificou-se uma variação considerável na velocidade de germinação e crescimento, que pode ser ilustrada pelas determinações de massa, indicando a grande variabilidade que se pode encontrar em estudos com espécies selvagens. Por isso, procurou-se caracterizar citoquimicamente um padrão morfológico de crescimento e desenvolvimento que permitisse uma visualização mais clara destes processos. Foi possível observar diferenças no padrão morfológico dos compostos de reserva, já a partir de 48 horas de embebição, seguindo-se um período variável de tempo sem modificações muito evidentes, até a protrusão da radícula. Selecionaram-se assim, 7 estádios de crescimento com base no comprimento do eixo hipocótilo-radicular. Nesse material não foram verificadas diferenças citoquímicas marcantes até 20 mm de comprimento, o mesmo ocorrendo no intervalo de 20 e 50 mm. Dessa forma, dos 7 estádios inicialmente estabelecidos foram selecionados 5, a saber: sementes quiescentes (E0); sementes com 48 horas de embebição (E1); protrusão da radícula (E2); eixo hipocótilo-radicular até 20 mm (E3) e 50 mm (E4). Nos diferentes métodos de coloração utilizados no estudo citoquímico, pode-se observar em E0, a uniformidade do conteúdo citoplasmático, com um aspecto globular e agregado. A partir de 48 horas de embebição (E1) já pôde ser observada uma separação dos glóbulos citoplasmáticos principalmente nas células da região central dos cotilédones. Essa desagregação se acentuou nos estádios E2 e E3 e no estádio E4 esses glóbulos se apresentaram maiores, em menor quantidade, com um formato circular bem definido. À diferença dos cotilédones esse processo nos eixos embrionários foi mais rápido. A natureza protéica do material no interior desses corpos foi demonstrada por métodos específIcos, evidenciando-se a natureza hidrofóbica de seu envoltório. As paredes celulares em geral mostraram aumento da disponilidade de radicais aniônicos no decorrer dos estádios. Juntamente com as determinações citoquímicas pôde-se constatar bioquimicamente a presença de uma grande quantidade de reserva lipídica nos cotilédones e principalmente nos eixos em E0, havendo redução deste conteúdo em estádios mais avançados. A composição dos ácidos graxos revelou a presença significativa dos ácidos linoléico, linolênico, esteárico+oléico e palmítico. Nos cotilédones a determinação do conteúdo protéico mostrou teores em tomo de 15% de sua massa fresca e seca. As sementes dessa espécie podem então ser classificadas como oleaginosas, com um conteúdo proteico razoável e pouca quantidade de carboidratos solúveis e amido
Doutorado
Biologia Vegetal
Doutor em Ciências
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Carvalho, Danila Blanco de. "Estudo da associação entre o sistema histo-sangüíneo ABO e a malária por Plasmodium falciparum na Amazônia brasileira /." São José do Rio Preto : [s.n.], 2008. http://hdl.handle.net/11449/94836.
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Resumo: O sistema sangüíneo ABO (sABO) é o mais importante sistema na compatibilidade de grupos sangüíneos. Muitas pesquisas têm mostrado associações deste sistema com várias doenças infecciosas, inclusive a malária. Este estudo avaliou a associação entre os genótipos do sistema histo-sangüíneo ABO e a malária não grave causada pelo Plasmodium falciparum. A genotipagem dos grupos sangüíneos do sistema ABO foi feita de acordo com o protocolo de PCR/ RFLP, em amostras de indivíduos maláricos e não maláricos de áreas da Amazônia brasileira. O genótipo homozigoto ABO*O01O01 foi prevalente tanto nos maláricos quanto nos doadores de sangue. O genótipo ABO*AB representou cerca de 3% da população infectada e 5% da não infectada. Não foram verificadas diferenças estatisticamente significantes na comparação das freqüências alélicas e genotípicas do sABO entre pacientes e grupo controle, mesmo quando foram analisados apenas indivíduos com infecções puras de P. falciparum. A freqüência do sABO na Amazônia brasileira pode estar relacionada com a baixa freqüência de malária grave pelo P. falciparum. Portanto, os genótipos encontrados no sistema ABO dos indivíduos maláricos e não maláricos pode promover relevantes informações, para o entendimento da epidemiologia da malária grave por P. falciparum na Amazônia brasileira.Abstract: The ABO blood system (sABO) is the most important system on the blood groups compatibility. Several studies have shown its associations with various infectious diseases, including malaria. This study evaluated the association between the ABO histo-blood genotypes and non-severe malaria caused by Plasmodium falciparum. PCR/RFLP protocol had be used for both ABO blood group system genotyping in malaria suffering individuals and blood donors, from malaria areas of the Brazilian Amazon. The homozygous genotype ABO*O01O01 was prevalent in both malaria and the blood donors. The genotype ABO*AB represented about 3% of the infected population and 5% of non-infected. No statistically significant differences were observed in sABO genotypic and allelic frequencies of patients and the control group, even when individuals were analyzed only with pure infection of P. falciparum. The frequency of sABO in the Brazilian Amazon may be related to the low frequency of non-severe malaria P. falciparum. Therefore, the genotypes found in the ABO blood system in malaric and non-malaric individuals can promote relevant information for the understanding of the severe malaria by P. falciparum epidemiology in the Brazilian Amazon. Keywords: Malaria; ABO blood group system; Plasmodium falciparum.
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Ait, Barka Essaïd. "Comportement des appareils caulinaires et gemmaires des vignes champenoises aux gelees d'hiver et de printemps : analyses histo-physiologiques." Reims, 1993. http://www.theses.fr/1993REIMS022.
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La progression du gel et les consequences metaboliques et structurales de la formation de glace au sein de l'appareil caulinogemmaire des vignes champenoises (vitis vinifera l. ) au cours des gelees d'hiver et de printemps ont ete etudies a l'aide de techniques physiques, biochimiques et histo-cytologiques. La prise en glace des tissus qui debute dans le sarment, gagne la base du bourgeon latent hiverne, puis ses composants primaire et secondaire. Le profil de cristallisation des sarments apparait bi-exothermique, celui des bourgeons latents est tri-exothermique. Ce dernier, a l'approche de la periode de debourrement devient mono-exothermique ce qui exprime une prise en glace brutale et totale de l'ensemble des tissus gemmaires due a la perte de leur capacite a developper le phenomene de surfusion. La nature et la localisation des degats tissulaires et cellulaires engendres par le froid dependent des temperatures de cristallisation et de l'etat physiologique de l'organe. Seul le bourgeon en cours de debourrement a montre des variations significatives de sa teneur en proline en fonction de la temperature
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Corkidi, Blanco Gabriel. "Système d'analyse de préparations histologiques par imagerie numérique : Histo 200 : application à l'étude physiopathologique de la maladie de Parkinson." Paris 12, 1989. http://www.theses.fr/1989PA120058.
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La recherche a conduit au developpement d'un systeme de traitement d'images specifiquement concu pour l'analyse interactif de preparations histologiques, qui repose sur une methodologie novatrice d'analyse semi-automatique de preparations de grande dimension (6. 46. 4 cm). Cette methodologie permet de combiner en temps reel les informations macroscopiques (par exemple les zones d'interet) avec l'analyse a fort grossissement. Le logiciel generalise ces concepts aux fonctions telles que le denombrement d'objets, la morphometrie et la mesure de densite optique (application en histoautoradiographie). Dans ce travail, l'evaluation du systeme a ete realise sur une application particuliere: etude comparative de divers types de neurones dopaminergiques du mesencephale ventral humain normal et pathologique (maladie de parkinson). Cette etude nous a permis de quantifier et de localiser les neurones preferentiellement leses parmi differentes regions neuroanatomiques chez le sujet parkinsonien. Il a ete remarque au cours de cette etude, qu'un nouveau parametre semblerait etre correle a la mort de ces neurones: l'orientation des corps neuronaux dans la substantia nigra
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Adler, Sophie. "Impacts histo-morphologiques et biochimiques de la mutation laurina sur les graines et les plantules de Coffea arabica L." Thesis, La Réunion, 2014. http://www.theses.fr/2014LARE0027/document.
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Coffea arabica var. Laurina (Bourbon pointu, BP) est un mutant naturel de C. arabica ‘Bourbon’ (B). La mutation laurina est récessive, monolocus, Mendélienne et ses effets pléiotropiques. Ces effets ont été étudiés sur le développement des graines et chez les plantules en histologie, morphologie et biochimie. La standardisation de la croissance en taille des graines était primordiale, notamment pour l'étude fine de la composition en polysaccharides pariétaux (PP). Les stades (st) de développement ont été reliés à l'âge des fruits et leur aspect histo-morphologique (st 1-2, 3, 4 : croissance ; st 5 à 7 : maturation). L'étude de la composition en PP a mis en évidence trois phases : φ 1 (st 1-2, 3), φ 2 (st 3, 4, première partie du 5), et φ 3 (deuxième partie du 5, st 6 et 7). La mutation affecte les φ 1 et 2, probablement par le biais du périsperme. Chez la plantule, la mutation affecte la longueur des hypocotyles exposés à la lumière en provoquant le semi-nanisme de BP par rapport à B par un nombre moindre de cellules. Les teneurs en ABA, auxine et cytokinines sont aussi touchées. La composition en PP et en acides chlorogéniques (CQA) n'est affectée ni par la mutation, ni par la lumière pendant la croissance des plantules (sauf pour les CQA ses racines). Un effet-organe est mis en évidence. La mutation réduit la teneur en caféine (CAF) et la différence variétale est présente à la lumière et à l'obscurité. Ces résultats permettent de mieux caractériser les effets pléiotropiques de la mutation laurina. Ils permettent une meilleure 1/ compréhension de l'évolution des PP de la graine au cours de son développement et 2/ connaissance des hormones, CAF, CQA et PP des plantulesCoffea arabica var Laurina (Bourbon pointu, BP), is a natural mutant of C. arabica ‘Bourbon’ (B). The laurina mutation is recessive, monolocus and Mendelian, with pleiotropic effects. This work highlighted new effects of this mutation, with histological, morphological and biochemical approaches on seeds development and on seedlings. Simultaneous fitting of growth curves of B and BP and their standardization allow comparisons of these varieties and fine study of polysaccharide cell wall (PCW) composition over time. Thus, anatomic specificities, development stages (st) and fruit age were linked (growth: st 1-2 to 4; maturation: st 5 to 7). When taking in account the CWP evolution in time-course, three phases occurred: φ 1 (st 1-2, 3), φ 2 (st 3, 4 and the beginning of 5), and φ 3 (the end of 5, st 6, 7). The two first were affected by the mutation probably through maternal effect (perisperm). In seedlings, the hypocotyls semi-dwarfism in light growth condition is an effect of the mutation and was due to a lower cell number than in B. The phytohormones content was also affected (ABA, auxine, cytokinins). But, neither CWP composition nor chlorogenics acids (CQA) content were affected by the mutation, or growth condition (expected for CQA content in roots). The composition in CWP and CQA was different depending on organs. Finally, caffeine (CAF) content was affected by the mutation in light or darkness conditions. These results represent an important step in 1/ the characterization of the pleiotropic effects of the laurina mutation, 2/ the understanding of the CWP evolution in seed time course, and 3/ new knowledge on seedlings (hormone, CAF, CQA and CWP composition)
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De, Resende Matta Marcos Fernando. "Comparaison du système immunitaire entre deux souches de poulets histo-compatibles, résistantes et sensibles à la maladie de Marek." Grenoble 2 : ANRT, 1987. http://catalogue.bnf.fr/ark:/12148/cb37604466c.
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Lauwrens, Jennifer. "The contested relationship between art history and visual culture studies A South African perspective /." Diss., Pretoria : [s.n.], 2006. http://upetd.up.ac.za/thesis/available/etd-05222007-133343.
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