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Journal articles on the topic 'Histochemical staining'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 April 2022

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1

Cowden, Ronald R., J. James, and J. Tas. "Histochemical Protein Staining Methods." Transactions of the American Microscopical Society 104, no. 4 (1985): 400. http://dx.doi.org/10.2307/3226494.

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2

MATOSZ, Bianca, Flavia RUXANDA, Cristian RATIU, Adrian Florin GAL, and Viorel MICLAUS. "Presence of Granular Ducts in Mandibular Gland in Rabbit." Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine 74, no. 1 (2017): 92. http://dx.doi.org/10.15835/buasvmcn-vm:12614.

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The present study focuses on the intralobular ducts present in rabbit mandibular gland, from a histological and histochemical point of view. We harvested mandibular gland samples from five rabbits (approximately six month old), which were paraffin embedded and subsequently stained for histological investigation with hematoxylin-eosin. PAS and Alcian blue reactions were used for histochemical assessment. Results show that mandibular gland in rabbit contains one type of acini, namely serous. Concerning the intralobular ducts, there were three types identified: intercalated, granular and striated

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3

Ferrante, R. J., N. W. Kowall, J. B. Martin та E. P. Richardson. "HISTOCHEMICAL STAINING PATTERNS IN HUNTINGTONʼS DISEASE". Journal of Neuropathology and Experimental Neurology 45, № 3 (1986): 335. http://dx.doi.org/10.1097/00005072-198605000-00067.

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4

Youngberg, George A., Ellen D. B. Wallen, and Tamar A. Giorgadze. "Narrow-Spectrum Histochemical Staining of Fungi." Archives of Pathology & Laboratory Medicine 127, no. 11 (2003): 1529–30. http://dx.doi.org/10.5858/2003-127-1529-nhsof.

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5

Thornburg, L. P., M. Beissenherz, M. Dolan, and M. F. Raisbeck. "Histochemical Demonstration of Copper and Copper-Associated Protein in the Canine Liver." Veterinary Pathology 22, no. 4 (1985): 327–32. http://dx.doi.org/10.1177/030098588502200405.

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Three different histochemical methods for copper detection were compared. Atomic absorption analysis was used to substantiate the tissue stains. There was good correlation between rhodanine staining and rubeanic acid-stained tissue sections. The orcein reaction for copper-associated protein did not consistently correlate with the methods demonstrating copper. Prolonged staining (72 hours) with rubeanic acid more consistently and clearly detected increased copper in canine livers than did staining with rhodanine. Seventy-two hour staining with rubeanic acid is the method of choice for histochem

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6

Simoes, Jose Pedro Cannas, and Polly Schoning. "Canine Mast Cell Tumors: A Comparison of Staining Techniques." Journal of Veterinary Diagnostic Investigation 6, no. 4 (1994): 458–65. http://dx.doi.org/10.1177/104063879400600410.

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Twelve histochemical methods; affinity staining with avidin peroxidase, wheat germ agglutinin, and concavalin-A agglutinin; and an immunohistochemical stain with Kpl (CD68) antibody were compared for their relative effectiveness in staining canine mast cell tumors. Stains were compared in 28 mast cell tumors and 19 histiocytomas. The effectiveness of the histochemical methods and the lectins decreased as the mast cells became less differentiated. None of the staining methods were positive on histiocytomas. Periodic acid-Schiff (PAS) gave positive results in a few cases of mast cell tumors wher

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7

Platt, Marvin S. "Imunohistochemical and Histochemical Staining of SIDS Case Tissues." American Journal of Forensic Medicine and Pathology 12, no. 4 (1991): 352. http://dx.doi.org/10.1097/00000433-199112000-00018.

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8

Bugelski, Peter J. "Sequential Histochemical Staining for Resident and Recruited Macrophages." Journal of Leukocyte Biology 38, no. 6 (1985): 687–96. http://dx.doi.org/10.1002/jlb.38.6.687.

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9

Kumar, Priti, Arvindhan Nagarajan та Pradeep D. Uchil. "Histochemical Staining of Cell Monolayers for β-Galactosidase". Cold Spring Harbor Protocols 2019, № 3 (2019): pdb.prot095422. http://dx.doi.org/10.1101/pdb.prot095422.

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10

Dariush Fahimi, H. "Peroxisomes: 40 years of histochemical staining, personal reminiscences." Histochemistry and Cell Biology 131, no. 4 (2009): 437–40. http://dx.doi.org/10.1007/s00418-009-0562-8.

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11

Katoh, Kazuo. "Microwave-Assisted Tissue Preparation for Rapid Fixation, Decalcification, Antigen Retrieval, Cryosectioning, and Immunostaining." International Journal of Cell Biology 2016 (2016): 1–9. http://dx.doi.org/10.1155/2016/7076910.

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Microwave irradiation of tissue during fixation and subsequent histochemical staining procedures significantly reduces the time required for incubation in fixation and staining solutions. Minimizing the incubation time in fixative reduces disruption of tissue morphology, and reducing the incubation time in staining solution or antibody solution decreases nonspecific labeling. Reduction of incubation time in staining solution also decreases the level of background noise. Microwave-assisted tissue preparation is applicable for tissue fixation, decalcification of bone tissues, treatment of adipos

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12

LeVine, Steven M., Hao Zhu, and Sarah E. Tague. "A Simplified Method for the Histochemical Detection of Iron in Paraffin Sections: Intracellular Iron Deposits in Central Nervous System Tissue." ASN Neuro 13 (January 2021): 175909142098216. http://dx.doi.org/10.1177/1759091420982169.

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Although all cells contain iron, most histochemical methods fail to reveal the presence of iron within many cells of the central nervous system (CNS), particularly neurons. Previously, a sensitive method was developed that limited the extraction of iron in paraffin sections, and this method revealed staining within neurons. However, the staining was often too robust making it difficult to discern discrete intracellular structures. In 1970, a study incorporated acetone in an iron histochemical procedure to facilitate the demarcation of staining features. In the present study, both acetone and l

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13

Couffinhal, Thierry, Marianne Kearney, Alison Sullivan, Marcy Silver, Yukio Tsurumi, and Jeffrey M. Isner. "Histochemical Staining Following LacZ Gene Transfer Underestimates Transfection Efficiency." Human Gene Therapy 8, no. 8 (1997): 929–34. http://dx.doi.org/10.1089/hum.1997.8.8-929.

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14

Danielson, Constance F., Ted Bloch, Geoffrey G. Brown, and Don-John Summerlin. "The Effect of Microwave Processing on Histochemical Staining Reactions." Journal of Histotechnology 13, no. 3 (1990): 181–83. http://dx.doi.org/10.1179/his.1990.13.3.181.

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15

Fang, S., J. Christensen, J. L. Conklin, J. A. Murray, and G. Clark. "Roles of Triton X-100 in NADPH-diaphorase histochemistry." Journal of Histochemistry & Cytochemistry 42, no. 11 (1994): 1519–24. http://dx.doi.org/10.1177/42.11.7930535.

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Triton X-100 is widely but not universally used in NADPH-diaphorase histochemical staining. We investigated its effect on the staining and examined nitroblue diformazan (NBF) production under the influence of Triton X-100. Exposure of opossum esophagus, intestine, and colon tissues to Triton X-100 before staining enhanced staining of nerve cells and fibers and suppressed staining of non-neural structures. Long exposures and high concentrations nearly abolished the staining of non-neural structures and decreased the staining of nerves. The use of an incubation medium containing Triton X-100 ach

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16

Westphal, C., H. Böhme, and D. Frösch. "Glycogen staining on sections of aqueous-embedded cyanobacteria and muscle." Journal of Histochemistry & Cytochemistry 33, no. 11 (1985): 1180–81. http://dx.doi.org/10.1177/33.11.2414363.

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We offer histochemical evidence that glycogen is preserved in the cytoplasm of cyanobacteria and muscle specimens that have been embedded in the water-soluble melamine resin Nanoplast for electron microscopy.

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17

Schimmer, Craig A., and Stephen C. Landers. "Histochemical Study of the Progenetic TrematodeAlloglossidium renale." Journal of Parasitology Research 2014 (2014): 1–8. http://dx.doi.org/10.1155/2014/245769.

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A histochemical study of the progenetic trematodeAlloglossidium renalehas demonstrated the absence of any secreted material between the adult worm and the host (freshwater shrimp) antennal gland tubules. Host tissue is affected only by the compression, abrasion, and ingestion by the parasite, and host tubule cells near the worm have the same staining patterns as those distant from the parasite. The trematode sometimes dies within the host, leaving a necrotic mass whose histochemical staining differs significantly from the living organism. In the necrotic mass, the only recognizable features we

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18

Nesa, Francesco, Luca Poggi, Stefano Ferrero, and Alessandro Del Gobbo. "Thyroid Follicular Hyperplasia Associated With Massive Extracellular Mucin Deposition." International Journal of Surgical Pathology 25, no. 6 (2017): 533–35. http://dx.doi.org/10.1177/1066896917696747.

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Extensive extracellular mucin deposition is a rare pathological thyroid condition with 6 cases described in literature so far. We report another case of a 67-year-old woman, discussing histopathological features, and review the literature. Our findings showed a diffuse mucin deposition in the stromal compartment of thyroid parenchyma. Histochemical stainings showed positivity for Alcian blue staining, but not for periodic acid–Shiff staining. Our case is peculiar because this mucin deposition was associated with benign nodular hyperplasia, in contrast with the other 6 reports, which described

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19

de Lima, Amanda L. R., Carmelita C. B. Cavalcanti, Mariana C. C. Silva, et al. "Histochemical Evaluation of Human Prostatic Tissues withCratylia mollisSeed Lectin." Journal of Biomedicine and Biotechnology 2010 (2010): 1–6. http://dx.doi.org/10.1155/2010/179817.

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Lectins, proteins which selectively recognize carbohydrates, have been used in histochemistry for the evaluation of changes in glycosylation in processes of cellular differentiation and/or dedifferentiation.Cratylia mollisseed lectins (Cramoll 1,4 and Cramoll 3), conjugated to horseradish peroxidase, were used as histochemical probes in human prostate tissues: normal (NP), hyperplasia (BPH), and prostate carcinoma (PCa). The staining pattern of Con-A and Cramoll 1,4 in BPH was more intense than in NP. These lectins also showed staining differences between BPH and PCa; the latter showing decrea

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20

Vladutiu, Georgirene D., and Reid R. Heffner. "Succinate Dehydrogenase Deficiency." Archives of Pathology & Laboratory Medicine 124, no. 12 (2000): 1755–58. http://dx.doi.org/10.5858/2000-124-1755-sdd.

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Abstract Background.—Partial succinate dehydrogenase deficiency (15% to 50% of normal reference enzyme activity) in skeletal muscle causes mitochondrial myopathy with various symptoms, for example, brain involvement, cardiomyopathy, and/or exercise intolerance. The deficiency may be isolated or may coexist with other respiratory-chain enzyme defects. The histopathologic assessment of succinate dehydrogenase activity in muscle biopsies of patients with suspected mitochondrial myopathies has focused on the finding of increased staining, usually in ragged-red fibers, rather than on reduced staini

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21

Hedreen, J. C., S. J. Bacon, and D. L. Price. "A modified histochemical technique to visualize acetylcholinesterase-containing axons." Journal of Histochemistry & Cytochemistry 33, no. 2 (1985): 134–40. http://dx.doi.org/10.1177/33.2.2578498.

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An improved histochemical method for light microscopic demonstration of acetylcholinesterase (AChE) has been developed. Axonal, dendritic, and perikaryal staining are well delineated, both in areas of low AChE content, such as cerebral cortex, and in areas of high AChE content, such as neostriatum. Axonal staining, including arborizations, stands out against a clear background devoid of diffuse reaction product.

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22

DelGaudio, John M., William R. Carroll, James J. Sciote, and Ramon M. Esclamado. "Atypical Myosin Heavy Chain in Rat Laryngeal Muscle." Annals of Otology, Rhinology & Laryngology 104, no. 3 (1995): 237–45. http://dx.doi.org/10.1177/000348949510400310.

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The myosin content of rat posterior cricoarytenoid and thyroarytenoid muscles was described by means of histochemical, immunohistochemical, and electrophoretic techniques. Laryngeal muscles were dissected and frozen, together with other muscles (extraocular, diaphragm, extensor digitorum longus, and soleus) for comparative purposes, then sectioned serially and stained: 1) histochemically for myofibrillar adenosine triphosphatase reactivity and 2) immunohistochemically for myosin heavy chain (MHC) content with six different antibodies. Other portions of the muscle samples were electrophoresed b

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23

Patel, V. M., L. A. Heinel, J. J. Provencio, P. E. Vinall, M. S. Kramer, and R. H. Rosenwasser. "Validation of image analysis for enzyme histochemical and immunocytochemical staining." Biotechnic & Histochemistry 77, no. 4 (2002): 213–21. http://dx.doi.org/10.1080/bih.77.4.213.221.

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24

Huang, Jiayi, Yi Hou, Tiancong Ma, et al. "A Novel Histochemical Staining Approach for Rare-Earth-Based Nanoprobes." Advanced Therapeutics 1, no. 1 (2018): 1800005. http://dx.doi.org/10.1002/adtp.201800005.

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25

Sumi, Y., Masanori T. Itoh, Takeshi Muraki, and Takuro Suzuki. "Histochemical staining of cadmium with 2-(8-quinolylazo)-4,5-diphenylimidazole." Histochemistry and Cell Biology 106, no. 2 (1996): 223–27. http://dx.doi.org/10.1007/s004180050035.

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26

Manners, Ruth M., and Roy O. Weller. "Histochemical staining of orbicularis oculi muscle in ectropion and entropion." Eye 8, no. 3 (1994): 332–35. http://dx.doi.org/10.1038/eye.1994.68.

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27

Patel, V. M., L. A. Heinel, J. J. Provencio, P. E. Vinall, M. S. Kramer, and R. H. Rosenwasser. "Validation of image analysis for enzyme histochemical and immunocytochemical staining." Biotechnic and Histochemistry 77, no. 4 (2002): 213–21. http://dx.doi.org/10.1080/714028196.

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28

Sumi, Yawara, Masanori T. Itoh, Takeshi Muraki, and Takuro Suzuki. "Histochemical staining of cadmium with 2-(8-quinolylazo)-4,5-diphenylimidazole." Histochemistry and Cell Biology 106, no. 2 (1996): 223–27. http://dx.doi.org/10.1007/bf02484404.

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29

Yang, Yan, Yiming He, Li Han, et al. "Application of histochemical stains for rapid qualitative analysis of the lignin content in multiple wood species." BioResources 15, no. 2 (2020): 3524–33. http://dx.doi.org/10.15376/biores.15.2.3524-3533.

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Rapid qualitative analysis was used to determine the influence of the lignin content of wood cell walls on the compression and bending properties of multiple wood species. The lignin type and cell wall content of Cunninghamia lanceolate, Fagus longipetiolata, Betula alnoides, Fraxinus mandshurica, and Tectona grandis was analyzed via histochemical staining, which included: the Mäule staining reaction, the Weisner staining reaction, and a fluorescence reaction. The results showed that the more red the Mäule staining reaction was, the greater the Syringyl lignin (S-type lignin) content was, and

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30

Soliman, Soha A., Basma Mohamed Kamal, Alaa S. Abuo-Elhmad, and Hanan H. Abd-Elhafeez. "Morphological and Histochemical Characterization of the Dermal Plates of Pleco (Hypostomus plecostomus)." Microscopy and Microanalysis 26, no. 3 (2020): 551–66. http://dx.doi.org/10.1017/s1431927620001476.

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AbstractStudying the dermal skeleton in fish is valuable for phylogenetic specification. The current study describes the detailed structure of the plecostomus dermal skeleton, including its morphogenesis and distribution in the skin. The denticles have a crown and a basal part and are embedded in bony depressions, to which they are attached by denticle ligaments. During denticle morphogenesis, denticle papillae formed from denticle precursor cells align in two cellular layers: an outer ameloblast precursor layer and an inner odontoblast precursor layer. The ameloblast precursors and odontoblas

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31

Sosnicki, A. A., G. J. Lutz, L. C. Rome, and D. O. Goble. "Histochemical and molecular determination of fiber types in chemically skinned single equine skeletal muscle fibers." Journal of Histochemistry & Cytochemistry 37, no. 11 (1989): 1731–38. http://dx.doi.org/10.1177/37.11.2530270.

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Until now, there has been no reliable method for histochemical determination of fiber types of single skinned muscle fibers. The major problem arises from the fact that most histochemical techniques use cross-sections of a large group of fibers and compare a given fiber with those surrounding it. This is not possible with a single skinned fiber which has been separated from a bundle to be used for mechanical analysis. A further problem is that the skinning procedure itself may alter the staining pattern. We have developed a procedure by which multiple cross-sections of single skinned fibers ca

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32

Chandanwale, Shirish S., Shruti S. Vimal, Mohit Rajpal, and Neha Mishra. "A Unique Case of Diffuse Histiocytic Proliferations Mimicking Metastatic Clear Cell Carcinoma in the Hydrocele Sac." Journal of Laboratory Physicians 6, no. 01 (2014): 043–45. http://dx.doi.org/10.4103/0974-2727.129091.

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ABSTRACTReactive histiocytic proliferations are extremely rare in paratesticular structures. Nodular histiocytic proliferations have been described in mesothelial-lined locations and only at few nonmesothelial sites. Diffuse histiocytic proliferations are described only in the pelvic peritoneum. We report the first case of diffuse histiocytic proliferation in the hydrocele sac of a 45-year-old man. Predominant histiocytes showed clear cytoplasm and signet ring-like change. Mucicarmin stain did not demonstrate mucin in the cytoplasm. Immunohistochemistry (IHC) staining showed nonspecific staini

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33

Bai, Zhi Kun, Xue Chao Tian, Yun Kao Cao, and Ji Long Luo. "Histochemical Study of early Embryo Implantation in Mice." Advanced Materials Research 641-642 (January 2013): 769–72. http://dx.doi.org/10.4028/www.scientific.net/amr.641-642.769.

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Implantation involves a complex process between the embryo and uterus and is crucial to further embryonic development. In this histochemical study, we investigated the early stage of embryo implantation and artiﬁcial decidualization in mice by the detection of total proteins and glycosaminoglycans using toluidine blue at pH 4.0 (TB) and Xylidine Ponceau at pH 2.5 (XP). TB staining showed mild metachromatic basophilia, which was more visible in the endometrial stroma around the implantation site in day 5 of pregnancy, and the level of glycosaminoglycans was more intense near the site of implant

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34

Andersson, G. N., and S. C. Marks. "Tartrate-resistant acid ATPase as a cytochemical marker for osteoclasts." Journal of Histochemistry & Cytochemistry 37, no. 1 (1989): 115–17. http://dx.doi.org/10.1177/37.1.2461980.

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We present a modified histochemical method for staining osteoclasts and adjacent mononuclear cells which takes advantage of the recently described substrate specificity for ATP of osteoclastic acid phosphatase. Staining of osteoclasts using ATP as substrate exhibits by light microscopy the same tartrate resistance as conventional acidic phosphatases, without the bone surface staining seen with other substrates. This feature, coupled with specific staining of fewer vicinal mononuclear cells, makes this method potentially useful for studying osteoclast ontogeny and function.

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35

Cabibi, Daniela, Fabrizio Bronte, Rossana Porcasi, et al. "Comparison of Histochemical Stainings in Evaluation of Liver Fibrosis and Correlation with Transient Elastography in Chronic Hepatitis." Analytical Cellular Pathology 2015 (2015): 1–7. http://dx.doi.org/10.1155/2015/431750.

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Background and Aim. The best staining to evaluate liver fibrosis in liver hepatitis is still a debated topic. This study aimed to compare Masson’s trichrome (MT), Sirius Red (SR), and orcein stainings in evaluating liver fibrosis in chronic HCV hepatitis (CHC) with semiquantitative and quantitative methods (Collagen Proportionate Area (CPA) by Digital Image Analysis (DIA)) and correlate them with transient elastography (TE).Methods. Liver stiffness evaluation of 111 consecutive patients with CHC was performed by TE. Semiquantitative staging by Metavir score system and CPA by DIA were assessed

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36

DE SOUSA, ALCEU MACHADO, CAROLINA RODRIGUES TEÓFILO, FRANCISCO ARTUR FORTE OLIVEIRA, et al. "Evaluation of Different Methods of Decalcification in Routine Staining, Histochemical Staining, and Immunohistochemical Assay of Bone." Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology 120, no. 2 (2015): e100. http://dx.doi.org/10.1016/j.oooo.2015.02.446.

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37

Hornstein, Eric P., Daniel L. Sambursky, and Steven C. Chamberlain. "Histochemical localization of acetylcholinesterase in the lateral eye and brain of Limulus polyphemus: Might acetylcholine be a neurotransmitter for lateral inhibition in the lateral eye?" Visual Neuroscience 11, no. 5 (1994): 989–1001. http://dx.doi.org/10.1017/s0952523800003928.

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AbstractThe distribution of acetylcholinesterase (AChE) in the lateral eye and brain of the horseshoe crab was investigated with histochemical means using standard controls to eliminate butyrylcholinesterase and nonspecific staining. Intense staining was observed in the neural plexus of the lateral compound eye, in the lateral optic nerve, and in various neuropils of the brain. Nerve fibers with moderate to weak staining were widespread in the brain. No sornata were stained in either the lateral eye or the brain. The distribution of acetylcholinesterase in the supraesophageal ganglia and nerve

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38

Jensen, Louise Kruse, Nicole Lind Henriksen, Thomas Bjarnsholt, Kasper Nørskov Kragh, and Henrik Elvang Jensen. "Combined Staining Techniques for Demonstration of Staphylococcus aureus Biofilm in Routine Histopathology." Journal of Bone and Joint Infection 3, no. 1 (2018): 27–36. http://dx.doi.org/10.7150/jbji.22799.

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Abstract. Aim: Visualization of Staphylococcus aureus biofilm using histochemical staining and combined histochemistry (HC) and immunohistochemistry (IHC).Methods: The ability of S. aureus S54F9 to form biofilm was tested in vitro. Hereafter, infected bone tissue was collected from two different porcine models of osteomyelitis inoculated with S. aureus strain S54F9. The infection time was five and fifteen days, respectively. Twenty-five different histochemical staining protocols were tested in order to find the stains that could identify extracellular biofilm matrix. Protocols with an optimal

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39

Taufikurohmah, Titik, I. Gusti Made Sanjaya, Afaf Baktir, and Achmad Syahrani. "Histochemical Changes Liver and Kidney of Mice Exposed to Mercury and Recovery with Nanogold." Molekul 11, no. 1 (2016): 80. http://dx.doi.org/10.20884/1.jm.2016.11.1.197.

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The background of this research is the circulation cosmetic with mercury that occur today in society. The problem of the research is that occur histochemical’s damage liver and kidney after exposure to mercury, and is that nanogold can recovery that damage. The pre-clinical study needed 24 mice (Mus muscullus) were divided into 6 groups, the control is A group, B group was exposed to mercury, Groups C, D, E and F after being exposed to mercury, than recovery by nanogold with concentration each of 5, 10, 15 and 20 ppm. Exposure was performed 1 week and 4 weeks of recovery. Necropsy of mice doin

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40

Bascal, Z. A., A. Montgomery, L. Holden-Dye, R. G. Williams, and R. J. Walker. "Histochemical mapping of NADPH diaphorase in the nervous system of the parasitic nematode Ascaris suum." Parasitology 110, no. 5 (1995): 625–37. http://dx.doi.org/10.1017/s0031182000065343.

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SUMMARYNADPH diaphorase has recently been discovered to be responsible for neuronal nitric oxide (NO) synthase activity in mammals. It thus serves as a histochemical marker for the localization of NO synthase in the nervous system. The histochemical technique was used to map out potential NO-producing neurones in the nervous system of the parasitic nematode, Ascaris suum. Positive staining for NADPH diaphorase was present in various parts of the central nervous system, in particular within selective cell bodies and fibres in the ventral ganglion, the retrovesicular ganglion, ventral and dorsal

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41

Marie, P. J., and M. Hott. "Histomorphometric identification of carbonic anhydrase in fetal rat bone embedded in glycolmethacrylate." Journal of Histochemistry & Cytochemistry 35, no. 2 (1987): 245–50. http://dx.doi.org/10.1177/35.2.3098835.

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Carbonic anhydrase was identified in bone-resorbing cells present in sections of fetal rat femur embedded in glycolmethacrylate. Using a slight modification of the Hansson's histochemical method, we demonstrated that most chondroclasts (91.8-95.4%) and osteoclasts (95.1-96.3%) display a positive histochemical reaction for carbonic anhydrase. This staining was consistently inhibited in the presence of very low concentrations (10(-6), 10(-7) M) of the specific inhibitor acetazolamide. The number of chondroclasts reacting for carbonic anhydrase was identical to the number of acid phosphatase-stai

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42

Streit, W. J. "An improved staining method for rat microglial cells using the lectin from Griffonia simplicifolia (GSA I-B4)." Journal of Histochemistry & Cytochemistry 38, no. 11 (1990): 1683–86. http://dx.doi.org/10.1177/38.11.2212623.

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A simple method for the lectin histochemical visualization of rat microglial cells is described. Advantages include ease of fixation of brain tissue using paraformaldehyde, and rapidity of tissue processing by vibratome sectioning. Furthermore, in addition to providing good structural preservation, the method achieves improved lectin binding, resulting in complete labeling of all microglial cells and in superior visualization of cellular processes. The lectin histochemical technique for rat microglia has the potential to be adapted to any mammalian species, and should prove valuable for neuros

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43

FLÜGEL, CASSANDRA, and ELKE LÜTJEN-DRECOLL. "Distribution of Carbonic Anhydrase in the Uterus of Late-Term Pregnant Spiny Dogfish (Squalus Acanthias)." Journal of Experimental Biology 158, no. 1 (1991): 531–37. http://dx.doi.org/10.1242/jeb.158.1.531.

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Carbonic anhydrase (CA) activity was localized in the late-term pregnant spiny dogfish uterus using the modified histochemical staining method of Hansson. Staining was found in the cytoplasm of red blood cells and in the membranes of the endometrial epithelial cells. Under the electron microscope, reaction products were seen in the basolateral membranes of the superficial cells, whereas the apical membranes were unstained. The cells of the underlying layer contacting the stromal capillaries showed staining in all their membranes. This staining pattern is similar to that found in other epitheli

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de Mera-Rodríguez, José Antonio, Guadalupe Álvarez-Hernán, Yolanda Gañán та ін. "Endogenous pH 6.0 β-Galactosidase Activity Is Linked to Neuronal Differentiation in the Olfactory Epithelium". Cells 11, № 2 (2022): 298. http://dx.doi.org/10.3390/cells11020298.

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The histochemical detection of β-galactosidase enzymatic activity at pH 6.0 (β-gal-pH6) is a widely used biomarker of cellular senescence in aging tissues. This histochemical assay also detects the presence of programmed cell senescence during specific time windows in degenerating structures of vertebrate embryos. However, it has recently been shown that this enzymatic activity is also enhanced in subpopulations of differentiating neurons in the developing central nervous system in vertebrates. The present study addressed the histochemical detection of β-gal-pH6 enzymatic activity in the devel

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Flanders, K. C., N. L. Thompson, D. S. Cissel, et al. "Transforming growth factor-beta 1: histochemical localization with antibodies to different epitopes." Journal of Cell Biology 108, no. 2 (1989): 653–60. http://dx.doi.org/10.1083/jcb.108.2.653.

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We have localized transforming growth factor-beta (TGF-beta) in many cells and tissues with immunohistochemical methods, using two polyclonal antisera raised to different synthetic preparations of a peptide corresponding to the amino-terminal 30 amino acids of TGF-beta 1. These two antibodies give distinct staining patterns; the staining by anti-CC(1-30) is intracellular. This differential staining pattern is consistently observed in several systems, including cultured tumor cells; mouse embryonic, neonatal, and adult tissues; bovine fibropapillomas; and human colon carcinomas. The extracellul

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Michaels, John E., and Robert R. Cardell. "Cytochemical localization of glycogen phosphorylase activity in rat liver." Proceedings, annual meeting, Electron Microscopy Society of America 51 (August 1, 1993): 298–99. http://dx.doi.org/10.1017/s0424820100147338.

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Glycogen phosphorylase (GP) is a key enzyme in liver glycogen breakdown. GP activity is altered with its state of phosphorylation. In the current study, the intralobular distribution of GP activity was observed histochemically in frozen sections of rat liver during fasting and after stimulation of glycogen synthesis.Normal and adrenalectomized (ADX) rats were fasted overnight to reduce liver glycogen to minimal levels. Fasted ADX rats received 2 mg dexamethasone (DEX) 0-8 h prior to sacrifice to stimulate glycogen synthesis. Liver was removed, rapidly frozen in isopentane cooled in liquid nitr

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Gowsik, Dr Kanimozhi, and Dr Archana V. "Comparison of various histochemical staining methods for identification of helicobacter pylori." Tropical Journal of Pathology and Microbiology 5, no. 9 (2019): 692–95. http://dx.doi.org/10.17511/jopm.2019.i09.12.

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Goso, Y., and K. Hotta. "Dot Blot Analysis of Rat Gastric Mucin Using Histochemical Staining Methods." Analytical Biochemistry 223, no. 2 (1994): 274–79. http://dx.doi.org/10.1006/abio.1994.1584.

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Sumi, Y., Masanori T. Itoh, Minoru Yoshida, and Yoshifumi Akama. "Highly sensitive chelating agents for histochemical staining of rare earth metals." Histochemistry and Cell Biology 112, no. 2 (1999): 179–82. http://dx.doi.org/10.1007/s004180050405.

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CHRISTENSEN, STEEN BACH, and INGE REIMANN. "DIFFERENTIAL HISTOCHEMICAL STAINING OF GLYCOSAMINOGLYCANS IN THE MATRIX OF OSTEOARTHRITIC CARTILAGE." Acta Pathologica Microbiologica Scandinavica Section A Pathology 88A, no. 1-6 (2009): 61–68. http://dx.doi.org/10.1111/j.1699-0463.1980.tb02467.x.

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