Relevant bibliographies by topics / Histological labelling

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  1. Journal articles
  2. Dissertations / Theses

Academic literature on the topic 'Histological labelling'

Author: Grafiati
Published: 4 June 2021
Last updated: 10 February 2022

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Journal articles on the topic "Histological labelling"

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Bhattacharya, Indroneel, Neepamanjari Barman, and Ranu Sarkar. "Ki67 Protein expression and correlation with the histological grade and pTNM stage of colorectal carcinoma." Panacea Journal of Medical Sciences 11, no. 2 (August 15, 2021): 204–8. http://dx.doi.org/10.18231/j.pjms.2021.044.

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: Colorectal cancer, is third most frequently encountered malignancy worldwide. Histological grade, stage, and proliferative index act as vital prognostic markers, playing a decisive role in patient care and prognosis. While histopathological examination can determine grade and stage, Ki67 protein expression by immunohistochemistry represents the proliferative capacity of the malignant cell population. : This Study was conducted to immunohistochemically analyze expression of Ki67 protein and its change with respect to different grades and stage of colorectal adenocarcinoma. : A total of 66 histologically diagnosed cases of colorectal carcinoma underwent histopathological examination followed by immunohistochemistry for Ki67 protein. : A statistically significant (p=0.04) correlation was obtained between Ki67 labelling index and histopathological grade, with higher values of Ki67 labelling index in poorly differentiated carcinomas (43.2±1.7). The Ki67 labelling index value was lowest in stage IV disease (11.4±2.4) with metastatic burden, with higher values in lower stage diseases, however this correlation was not found to be significant statistically (p=0.07). : The rate of cell division and proliferation measured by Ki-67 antibody is related to histological grade of the malignancy, demonstrating higher mitotic activity with loss of differentiation and anaplasia. Stage IV disease have lower mitotic activity, thus may be less amenable to cytostatic chemotherapy drugs, and require multimodality treatment with addition of radiotherapy and other drug regimes.
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Moore, H. C., K. M. Wood, M. S. Jackson, M. A. Lastowska, D. Hall, H. Imrie, C. P. F. Redfern, et al. "Histological profile of tumours from MYCN transgenic mice." Journal of Clinical Pathology 61, no. 10 (August 4, 2008): 1098–103. http://dx.doi.org/10.1136/jcp.2007.054627.

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Background:MYCN is the most commonly amplified gene in human neuroblastomas. This proto-oncogene has been overexpressed in a mouse model of the disease in order to explore the role of MYCN in this tumour.Aims:To report the histopathological features of neuroblastomas from MYCN transgenic mice.Methods:27 neuroblastomas from hemizygous transgenic mice and four tumours from homozygous mice were examined histologically; Ki67 and MYCN immunocytochemistry was performed in 24 tumours.Results:Tumours obtained from MYCN transgenic mice resembled human neuroblastomas, displaying many of the features associated with stroma-poor neuroblastoma, including heterogeneity of differentiation (but no overt ganglionic differentiation was seen), low levels of Schwannian stroma and a high mitosis karyorrhexis index. The tumours had a median Ki67 labelling index of 70%; all tumours expressed MYCN with a median labelling index of 68%. The most striking difference between the murine and human neuroblastomas was the presence of tingible body macrophages in the transgenic mouse tumours reflecting high levels of apoptosis. This has not previously been described in human or other murine neuroblastoma models.Conclusions:These studies highlight the histological similarities between tumours from MYCN transgenic mice and human neuroblastomas, and reaffirm their role as a valuable model to study the biology of aggressive human neuroblastoma.
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Verma, Kavita, Satya Raj Negi, and Sanjay Garhwal. "To study histopathological features, GFAP staining and Ki 67/ MIB-1 labelling index in diagnoses and histological grading of gliomas." Indian Journal of Forensic and Community Medicine 8, no. 2 (July 15, 2021): 115–19. http://dx.doi.org/10.18231/j.ijfcm.2021.023.

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: A group of tumors that develops in the brain and spinal cord is called glioma. Histologically gliomas are classified into astrocytoma, ependymoma, oligodendroglioma, brain stem glioma and oligoastrocytoma. The present study was conducted to study histological grading of gliomas and correlate it with patient’s age, sex, GFAP staining, role of the Ki-67/MIB-1 labelling indices (PIs). The present retrospective study was conducted on 50 biopsies received. All specimens were subjected to immunohistochemistory for GFAP and MIB-1 and correlated with WHO grading for glioma.: High incidence of glial tumours was seen in the 3 and 4 decade with slight male predominance (60%), with commonest site being frontal lobe. Glioblastomas (Grade IV) constitute the most common glial tumour. A statistically significant correlation was observed between GFAP staining and Ki-67/MIB-1 with histological grading.: The study can prove helpful in diagnosis and histological grading of gliomas and in planning treatment protocols and strategies.
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Erokhina, Maria V., Larisa N. Lepekha, Elena E. Voronezhskaya, Leonid P. Nezlin, Vadim G. Avdienko, and Atadzhan E. Ergeshov. "Application of Laser Scanning Confocal Microscopy for the Visualization of M. tuberculosis in Lung Tissue Samples with Weak Ziehl–Neelsen Staining." Journal of Clinical Medicine 8, no. 8 (August 7, 2019): 1185. http://dx.doi.org/10.3390/jcm8081185.

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One of the key requirements for the diagnosis of pulmonary tuberculosis is the identification of M. tuberculosis in tissue. In this paper, we present the advantages of specific fluorescent antibody labelling, combined with laser scanning confocal microscopy (LSCM), for the detection of M. tuberculosis in histological specimens of lung tissues. We demonstrate that the application of LSCM allows: (i) The automatic acquisition of images of the whole slice and, hence, the determination of regions for subsequent analysis; (ii) the acquisition of images of thick (20–40 μm) slices at high resolution; (iii) single bacteria identification; and (iv) 3D reconstruction, in order to obtain additional information about the distribution, size, and morphology of solitary M. tuberculosis; as well as their aggregates and colonies, in various regions of tuberculosis inflammation. LSCM allows for the discrimination of the non-specific fluorescence of bacteria-like particles and their aggregates presented in histological lung samples, from the specific fluorescence of labelled M. tuberculosis, using spectrum emission analysis. The applied method was effective in the identification of M. tuberculosis in lung histological samples with weak Ziehl–Neelsen staining. Altogether, combining immunofluorescent labelling with the application of LSCM visualization significantly increases the effectiveness of M. tuberculosis detection.
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MATTFELDT, T., S. ECKEL, F. FLEISCHER, and V. SCHMIDT. "Statistical analysis of labelling patterns of mammary carcinoma cell nuclei on histological sections." Journal of Microscopy 235, no. 1 (July 2009): 106–18. http://dx.doi.org/10.1111/j.1365-2818.2009.03187.x.

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Boulanger, Jenna J., William A. Staines, Véronique LeBlanc, Eve-Ling Khoo, Jacky Liang, and Claude Messier. "A simple histological technique to improve immunostaining when using DNA denaturation for BrdU labelling." Journal of Neuroscience Methods 259 (February 2016): 40–46. http://dx.doi.org/10.1016/j.jneumeth.2015.11.006.

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Strateva, M., and G. Penchev. "HISTOLOGICAL, PHYSICOCHEMICAL AND MICROBIOLOGICAL CHANGES IN FRESH AND FROZEN/THAWED FISH." BULGARIAN JOURNAL OF VETERINARY MEDICINE 23, no. 1 (2020): 69–80. http://dx.doi.org/10.15547/tjs.2020.01.012.

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The production and supply of fish as food is constantly growing worldwide. Various methods are applied to extend its shelf life, one of them being freezing. According to European Union legislation, the state of the food and its treatment must be indicated on the label. If the food had been frozen prior to marketing and then sold thawed, this information must be provided to the consumer by labelling it. Otherwise, this is considered a fraud to the consumer since freezing significantly degrades the quality of fish. Histological, physicochemical and microbiological changes in the muscle tissue of frozen fish occur. Different methods may be applied to distinguish between fresh and frozen and them thawed fish, of which histological examination is a reliable method.
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Liu, Y., G. Chen, Y. S. Liu, Z. Q. Wang, T. Zhu, and F. Qiao. "Distraction osteogenesis correction for tissue defects of cleft palate: histological, immunohistochemical and fluorescent labelling study." International Journal of Oral and Maxillofacial Surgery 38, no. 5 (May 2009): 456–57. http://dx.doi.org/10.1016/j.ijom.2009.03.216.

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Yahaya, B., G. McLachlan, and D. D. S. Collie. "BrdU Pulse LabellingIn Vivoto Characterise Cell Proliferation during Regeneration and Repair following Injury to the Airway Wall in Sheep." Scientific World Journal 2013 (2013): 1–5. http://dx.doi.org/10.1155/2013/871932.

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The response of S-phase cells labelled with bromodeoxyuridine (BrdU) in sheep airways undergoing repair in response to endobronchial brush biopsy was investigated in this study. Separate sites within the airway tree of anaesthetised sheep were biopsied at intervals prior to pulse labelling with BrdU, which was administered one hour prior to euthanasia. Both brushed and spatially disparate unbrushed (control) sites were carefully mapped, dissected, and processed to facilitate histological analysis of BrdU labelling. Our study indicated that the number and location of BrdU-labelled cells varied according to the age of the repairing injury. There was little evidence of cell proliferation in either control airway tissues or airway tissues examined six hours after injury. However, by days 1 and 3, BrdU-labelled cells were increased in number in the airway wall, both at the damaged site and in the regions flanking either side of the injury. Thereafter, cell proliferative activity largely declined by day 7 after injury, when consistent evidence of remodelling in the airway wall could be appreciated. This study successfully demonstrated the effectiveness ofin vivopulse labelling in tracking cell proliferation during repair which has a potential value in exploring the therapeutic utility of stem cell approaches in relevant lung disease models.
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Schatzker, J., G. Sumner-Smith, V. L. Fornasier, and J. R. Cockshutt. "Biological Fixation of a Porous-Coated, Metal-Backed Acetabular Component in Canine Total Hip Arthroplasty." Veterinary and Comparative Orthopaedics and Traumatology 01, no. 03/04 (October 1988): 141–45. http://dx.doi.org/10.1055/s-0038-1633277.

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A total hip surface replacement was successfully performed on eight adult dogs, using a porous-coated, cobalt-chromium- molybdenum alloy acetabular resurfacing component fixed to the acetabulum with 3.5 mm cortical screws. Functional, radiological, histological and fluorochrome labelling studies were used to assess tissue response to the implant. At four months after implantation, seven of the acetabular components were anchored with well organized fibrous tissue. Bone ingrowth had occurred in three components. Failures appeared to be due to technical difficulties encountered during the surgical procedure.
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Dissertations / Theses on the topic "Histological labelling"

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VIOLIN, KALAN B. "Implantes cerâmicos e metálicos - caracterização da osteointegração por imuno-histoquímica, lectina-histoquímica e marcadores fluorescentes." reponame:Repositório Institucional do IPEN, 2015. http://repositorio.ipen.br:8080/xmlui/handle/123456789/25300.

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Submitted by Claudinei Pracidelli (cpracide@ipen.br) on 2015-12-10T17:29:01Z No. of bitstreams: 0
Made available in DSpace on 2015-12-10T17:29:01Z (GMT). No. of bitstreams: 0
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Tese (Doutorado em Tecnologia Nuclear)
IPEN/T
Instituto de Pesquisas Energeticas e Nucleares - IPEN-CNEN/SP
FAPESP:10/20698-4
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Mounier, Françoise. "Ontogenèse du rein humain : étude immunohistochimie de marqueurs intra et extracellulaires de différenciation." Paris 6, 1986. http://www.theses.fr/1986PA066128.

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La distribution de différentes protéines a été étudiée au cours du développement du rein humain, à partir de la huitième semaine de vie fœtale. L'expression des différents marqueurs cellulaires et extracellulaires dépend essentiellement de la différenciation individuelle de chaque néphron.
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Roussel, Francis. "Marquage de l'endothélium vasculaire du chien par la lectine d'evonymus europaeus." Rouen, 1987. http://www.theses.fr/1987ROUES031.

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Cadusseau, Josette. "Proposition d'une nouvelle définition du noyau postérieur du thalamus chez le rat, d'après une étude hodologique." Poitiers, 1987. http://www.theses.fr/1987POIT2025.

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Ma, Wu. "Aspects structuraux et ultrastructuraux des projections spinales et trigeminales dans le thalamus et l'aire parabrachiale." Paris 6, 1987. http://www.theses.fr/1987PA066132.

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Miceli, Dom. "Organisation anatomo-fonctionnelle des afferences et efferences de l'hyperstriatum visuel des oiseaux." Paris 6, 1987. http://www.theses.fr/1987PA066188.

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Dutar, Patrick. "Les systèmes cholinergiques centraux chez le rat adulte et le rat âgé : étude des caractéristiques électrophysiologies et pharmacologiques." Paris 6, 1986. http://www.theses.fr/1986PA066464.

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Etude in vivo chez le rat adulte et âgé à l'aide d'approches multidisciplinaires des caractéristiques des neurons cholinergiques des voies septo-hippocampique et basalo-corticale. Les caractéristiques physiologiques ont été décrites par enregistrements extra et intracellulaires, les propriétés pharmacologiques par applications iontophorétiques, les modifications de ces propriétés ont été étudiées au cours du vieillissement. Enfin une étude anatomique par marquage histologique à la peroxydase a été menée pour définir la voie basalo-corticole.
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