Academic literature on the topic 'Histology, Pathological – Technique'

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Journal articles on the topic "Histology, Pathological – Technique"

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Zulli, Anthony, and James J. Liu. "A Novel Immunohistochemical Semiquantitative Technique for Endothelial Constitutive Nitric Oxide Synthase Immunoreactivity in Rat Coronary Artery." Journal of Histochemistry & Cytochemistry 46, no. 2 (1998): 257–62. http://dx.doi.org/10.1177/002215549804600215.

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It has been difficult to quantify protein production in small pathological specimens by conventional techniques. We describe a new method for semiquantification of immunohistochemical staining, which involves application of the enzyme-labeled avidin (LAB) technique, coupled with an ultra-sensitive and fast chemiluminescent substrate for alkaline phosphatase. The entire procedure can be completed in less than 3 hr. The final step involves X-ray film exposure for 30 min, and the optical density of the subsequent images is examined with a microcomputer imaging device. The optical densities are tr
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Montironi, Rodolfo, Antonio Lopez Beltran, Roberta Mazzucchelli, Liang Cheng, and Marina Scarpelli. "Handling of Radical Prostatectomy Specimens: Total Embedding with Large-Format Histology." International Journal of Breast Cancer 2012 (2012): 1–6. http://dx.doi.org/10.1155/2012/932784.

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A problem when handling radical prostatectomy specimens (RPS) is that cancer is often not visible at gross examination, and the tumor extent is always underestimated by the naked eye. The challenge is increased further by the fact that prostate cancer is a notoriously multifocal and heterogeneous tumor. For the pathologist, the safest method to avoid undersampling of cancer is evidently that the entire prostate is submitted. Even though whole mounts of sections from RPS appear not to be superior to sections from standard blocks in detecting adverse pathological features, their use has the grea
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Crocetti, Laura, Paola Scalise, Elena Bozzi, et al. "Microwave Ablation of Very-Early- and Early-Stage HCC: Efficacy Evaluation by Correlation with Histology after Liver Transplantation." Cancers 13, no. 14 (2021): 3420. http://dx.doi.org/10.3390/cancers13143420.

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Microwave (MW) ablation is a worldwide-diffused technique for the percutaneous ablation of hepatocellular carcinoma (HCC). Nevertheless, the efficacy of this technique still needs to be confirmed in pathological specimens. The purpose of this study was to evaluate the efficacy of MW ablation by correlation with histology in excised liver samples at the time of liver transplantation (LT). All patients with MW-ablated HCC who subsequently underwent LT between 2012 and 2020 were retrospectively evaluated. In the explanted livers, the treated lesions were evaluated at pathology, and the necrosis w
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Jonsson, R., A. L. Karlsson, and R. Holmdahl. "Demonstration of immunoreactive sites on cartilage after in vivo administration of biotinylated anti-type II collagen antibodies." Journal of Histochemistry & Cytochemistry 37, no. 2 (1989): 265–68. http://dx.doi.org/10.1177/37.2.2911008.

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Administration of biotinylated monoclonal antibodies provides the basis of a simple technique for identifying immunoreactive sites in vivo. Biotinylated anti-type II collagen antibodies were injected intraperitoneally into normal DBA/1 mice. The mice were sacrificed after 96 hr and the front paws removed and decalcified to allow tissue sectioning before snap-freezing. Binding of antibodies in vivo was visualized with affinity cytochemical staining using avidin-biotin-peroxidase complexes. Specific binding of antibodies to cartilaginous structures was seen after injection of 20-500 micrograms b
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Tuson, J. R., E. W. Pascoe, and D. A. Jacob. "A novel immunohistochemical technique for demonstration of specific binding of human monoclonal antibodies to human cryostat tissue sections." Journal of Histochemistry & Cytochemistry 38, no. 7 (1990): 923–26. http://dx.doi.org/10.1177/38.7.2355173.

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We describe a novel immunohistochemical technique which permits the detection of specific binding of human monoclonal antibodies (MAb) to cryostat sections of human tissues. The technique overcomes the problem of background staining caused by the presence of endogenous immunoglobulins in tissue sections. This is achieved by the formation of a molecular complex of the primary antibody (a human MAb), horseradish peroxidase-conjugated goat anti-human immunoglobulin, and normal human serum. This complex is then incubated with cryostat sections of human tissue, and binding of the complex is demonst
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Péley, G., J. Tóth, O. Csuka, I. Sinkovics, E. Farkas, and I. Köves. "Immunohistochemistry and Reverse Transcriptase Polymerase Chain Reaction on Sentinel Lymph Nodes can Improve the Accuracy of Nodal Staging in Breast Cancer Patients." International Journal of Biological Markers 16, no. 4 (2001): 227–32. http://dx.doi.org/10.1177/172460080101600401.

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In this study the nodal staging sensitivity of sentinel lymph node biopsy (SLNB) with detailed pathological and molecular biological examination has been investigated and compared to that of axillary lymph node dissection (ALND) with routine histological evaluation. Sentinel lymph nodes (SLNs) were removed by the dual-agent injection technique in 68 patients with primary, clinically node-negative breast cancer. Forty-seven patients had negative SLNs according to hematoxylin and eosin (H&E) staining. These H&E-negative SLNs were serially sectioned and examined at 250 μm levels by anticy
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Cauda, Franco, Andrea Nani, Donato Liloia, et al. "Interhemispheric co-alteration of brain homotopic regions." Brain Structure and Function 226, no. 7 (2021): 2181–204. http://dx.doi.org/10.1007/s00429-021-02318-4.

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AbstractAsymmetries in gray matter alterations raise important issues regarding the pathological co-alteration between hemispheres. Since homotopic areas are the most functionally connected sites between hemispheres and gray matter co-alterations depend on connectivity patterns, it is likely that this relationship might be mirrored in homologous interhemispheric co-altered areas. To explore this issue, we analyzed data of patients with Alzheimer’s disease, schizophrenia, bipolar disorder and depressive disorder from the BrainMap voxel-based morphometry database. We calculated a map showing the
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Chilosi, M., A. Mombello, L. Montagna, et al. "Multimarker immunohistochemical staining of calgranulins, chloroacetate esterase, and S100 for simultaneous demonstration of inflammatory cells on paraffin sections." Journal of Histochemistry & Cytochemistry 38, no. 11 (1990): 1669–75. http://dx.doi.org/10.1177/38.11.2212622.

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Mac387 monoclonal antibody (MAb) recognizes two calcium binding, myeloid-associated proteins, now termed calgranulins, expressed at high levels by neutrophils and monocytes. Calgranulins are related to migration inhibitory factor (MIF) and are lost in a few days from monocytes differentiated in vitro. This marker is therefore potentially useful to analyze macrophage heterogeneity and turnover in tissue sections. In this study, we developed an immunohistochemical multimarker technique, including calgranulin demonstration, suitable for analyzing different inflammatory cells on paraffin-embedded
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Heet, Vora, Malavia Manav, and Hirey Manav. "DEEP LEARNING BASED BREAST CANCER DETECTION USING ULTRASOUND IMAGE." Volume 8 Issue 11 8, no. 11 (2021): 10–13. https://doi.org/10.5281/zenodo.5712357.

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In the fields of healthcare and bioinformatics, the definition of breast cancer has been the topic of concern, since this is the second principal explanation for death from cancer in women. This form of study will only be achieved if a sample of breast tissue is extracted from the breast, evaluated, and analyzed under a microscope. In the histopathology lab, problems are detected by examining the specimens using trained and qualified pathologists, and identified with further investigation using special techniques. However, the ultrasound can incorrectly identify pathological changes or conditi
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Dinneen, Eoin, Aiman Haider, and Greg Shaw. "NeuroSAFE technique—pathological considerations and practical implications for guiding nerve‐sparing surgery in prostate cancer patients." Histopathology 77, no. 4 (2020): 536–38. http://dx.doi.org/10.1111/his.14183.

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Books on the topic "Histology, Pathological – Technique"

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Carson, Freida L. Histotechnology: A self-instructional text. 2nd ed. ASCP Press, 1997.

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Carson, Freida L. Histotechnology: A self instructional text. ASCP Press, 1990.

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Jumer, Patricia A. Microwave modified procedures for the histotechnician in an hour or less. I.S.A.C. Technologies, 1994.

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Sheehan, Dezna C. Theory and practice of histotechnology. 2nd ed. Battelle Press, 1987.

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Luna, Lee G. Histopathologic methods and color atlas of special stains and tissue artifacts. American Histolabs, 1992.

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Jasani, Bharat. Immunocytochemistry in diagnostic histopathology. Churchill Livingstone, 1993.

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Tadrous, Paul Joseph. Diagnostic criteria handbook in histopathology: A surgical pathology vade mecum. John Wiley & Sons, 2007.

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Lowe, D. G. Macro techniques in diagnostic histopathology. Wolfe Medical Publications, 1990.

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J, Cook D. Cellular pathology. Butterworth-Heinemann, 1998.

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Arthur Purdy Stout Society of Surgical Pathologists. Scientific Meeting. Proceedings of the Arthur Purdy Stout Society Symposium "New techniques in surgical pathology." And the Gastrointestinal Pathology Society Symposium "Gastrointestinal infections". Edited by Society for Hematopathology, Sternberg Stephen S, Arthur Purdy Stout Society of Surgical Pathologists., Gastrointestinal Pathology Society Symposium, and Gastrointestinal Pathology Society. Raven Press, 1988.

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Book chapters on the topic "Histology, Pathological – Technique"

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Fitzpatrick, John, Asif Muneer, Jean de la Rosette, and Thomas Powles. "Genitourinary cancers." In Oxford Textbook of Oncology. Oxford University Press, 2016. http://dx.doi.org/10.1093/med/9780199656103.003.0045.

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Genitourinary cancers include tumors of the penis, bladder, prostate, testes, kidneys and ureters. Penile cancer is a rare malignancy and the diagnosis is often delayed due to patient embarrassment. Penile-preserving surgical techniques have been developed to preserve penile length. Urothelial cell carcinoma is more often encountered in bladder (95%) than in the upper urinary tract (5%). This chapter will be divided according to the two main urothelial cell carcinoma topographies: bladder and upper urinary tract. Prostate cancer is second as far as cause of cancer death is concerned, and surgi
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Bhindi, Ravinay, and Keith M. Channon. "Virtual histology intravascular ultrasound and optical coherence tomography in percutaneous coronary intervention." In Oxford Textbook of Interventional Cardiology. Oxford University Press, 2010. http://dx.doi.org/10.1093/med/9780199569083.003.011.

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The inadequacies of angiography to identify and characterize coronary atherosclerosis were not fully appreciated until pathologic studies revealed that coronary atherosclerosis in patients with fatal myocardial infarction was typically diffuse and in many cases was accompanied by positive remodelling, without luminal stenosis. Pathologic studies also identified the critical pathophysiogical role of plaque rupture in coronary thrombosis, and the appreciation that plaque biology and composition, rather than luminal stenosis alone, were more critical determinants of plaque behaviour. Clinical and
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