Relevant bibliographies by topics / HIV, phenotypic assay, cell based assay

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Book chapters
  4. Conference papers
  5. Reports

Academic literature on the topic 'HIV, phenotypic assay, cell based assay'

Author: Grafiati
Published: 14 March 2023

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Journal articles on the topic "HIV, phenotypic assay, cell based assay"

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Trkola, Alexandra, Jamie Matthews, Cynthia Gordon, Tom Ketas, and John P. Moore. "A Cell Line-Based Neutralization Assay for Primary Human Immunodeficiency Virus Type 1 Isolates That Use either the CCR5 or the CXCR4 Coreceptor." Journal of Virology 73, no. 11 (1999): 8966–74. http://dx.doi.org/10.1128/jvi.73.11.8966-8974.1999.

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ABSTRACT We describe here a cell line-based assay for the evaluation of human immunodeficiency virus type 1 (HIV-1) neutralization. The assay is based on CEM.NKR cells, transfected to express the HIV-1 coreceptor CCR5 to supplement the endogenous expression of CD4 and the CXCR4 coreceptor. The resulting CEM.NKR-CCR5 cells efficiently replicate primary HIV-1 isolates of both R5 and X4 phenotypes. A comparison of the CEM.NKR-CCR5 cells with mitogen-activated peripheral blood mononuclear cells (PBMC) in neutralization assays with sera from HIV-1-infected individuals or specific anti-HIV-1 monoclo
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Teeranaipong, Phairote, Noriaki Hosoya, Ai Kawana-Tachikawa, et al. "Development of a rapid cell-fusion-based phenotypic HIV-1 tropism assay." Journal of the International AIDS Society 16, no. 1 (2013): 18723. http://dx.doi.org/10.7448/ias.16.1.18723.

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Kalu, Amare Worku, Nigus Fikrie Telele, Shambhu G. Aralaguppe, et al. "Coreceptor Tropism and Maraviroc Sensitivity of Clonally Derived Ethiopian HIV-1C Strains Using an in-house Phenotypic Assay and Commonly Used Genotypic Methods." Current HIV Research 16, no. 2 (2018): 113–20. http://dx.doi.org/10.2174/1570162x16666180515124836.

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Objectives:Genotypic Tropism Testing (GTT) tools are generally developed based on HIV-1 subtype B (HIV-1B) and used for HIV-1C as well but with a large discordance of prediction between different methods. We used an established phenotypic assay for comparison with GTT methods and for the determination of in vitro maraviroc sensitivity of pure R5-tropic and dual-tropic HIV-1C.Methods:Plasma was obtained from 58 HIV-1C infected Ethiopians. Envgp120 was cloned into a luciferase tagged NL4-3 plasmid. Phenotypic tropism was determined by in house method and the V3 sequences were analysed by five GT
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Nissley, Dwight V., Jessica Radzio, Zandrea Ambrose, et al. "Characterization of novel non-nucleoside reverse transcriptase (RT) inhibitor resistance mutations at residues 132 and 135 in the 51 kDa subunit of HIV-1 RT." Biochemical Journal 404, no. 1 (2007): 151–57. http://dx.doi.org/10.1042/bj20061814.

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Several rare and novel NNRTI [non-nucleoside reverse transcriptase (RT) inhibitor] resistance mutations were recently detected at codons 132 and 135 in RTs from clinical isolates using the yeast-based chimaeric TyHRT (Ty1/HIV-1 RT) phenotypic assay. Ile132 and Ile135 form part of the β7–β8 loop of HIV-1 RT (residues 132–140). To elucidate the contribution of these residues in RT structure–function and drug resistance, we constructed twelve recombinant enzymes harbouring mutations at codons 132 and 135–140. Several of the mutant enzymes exhibited reduced DNA polymerase activities. Using the yea
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Hertogs, Kurt, Marie-Pierre de Béthune, Veronica Miller, et al. "A Rapid Method for Simultaneous Detection of Phenotypic Resistance to Inhibitors of Protease and Reverse Transcriptase in Recombinant Human Immunodeficiency Virus Type 1 Isolates from Patients Treated with Antiretroviral Drugs." Antimicrobial Agents and Chemotherapy 42, no. 2 (1998): 269–76. http://dx.doi.org/10.1128/aac.42.2.269.

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ABSTRACT Combination therapy with protease (PR) and reverse transcriptase (RT) inhibitors can efficiently suppress human immunodeficiency virus (HIV) replication, but the emergence of drug-resistant variants correlates strongly with therapeutic failure. Here we describe a new method for high-throughput analysis of clinical samples that permits the simultaneous detection of HIV type 1 (HIV-1) phenotypic resistance to both RT and PR inhibitors by means of recombinant virus assay technology. HIV-1 RNA is extracted from plasma samples, and a 2.2-kb fragment containing the entire HIV-1 PR- and RT-c
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Buzon, Maria José, Itziar Erkizia, Christian Pou, et al. "A non-infectious cell-based phenotypic assay for the assessment of HIV-1 susceptibility to protease inhibitors." Journal of Antimicrobial Chemotherapy 67, no. 1 (2011): 32–38. http://dx.doi.org/10.1093/jac/dkr433.

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Carbonari, M., M. Cibati, M. Cherchi, et al. "Detection and characterization of apoptotic peripheral blood lymphocytes in human immunodeficiency virus infection and cancer chemotherapy by a novel flow immunocytometric method." Blood 83, no. 5 (1994): 1268–77. http://dx.doi.org/10.1182/blood.v83.5.1268.1268.

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Abstract We have developed a quantitative and sensitive flow cytometric method for the detection of human apoptotic lymphocytes that, unlike previously described assays, allows their identification in mixed populations of peripheral blood leukocytes as well as their immunophenotyping. Apoptotic lymphocytes are identified on the basis of peculiar light scatter changes, reflecting their smaller size and their modified nucleus/cytoplasm organization, and of the decreased expression of surface CD45 molecules. Based on these criteria, apoptotic lymphocytes generated by exposure to ionizing radiatio
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Carbonari, M., M. Cibati, M. Cherchi, et al. "Detection and characterization of apoptotic peripheral blood lymphocytes in human immunodeficiency virus infection and cancer chemotherapy by a novel flow immunocytometric method." Blood 83, no. 5 (1994): 1268–77. http://dx.doi.org/10.1182/blood.v83.5.1268.bloodjournal8351268.

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We have developed a quantitative and sensitive flow cytometric method for the detection of human apoptotic lymphocytes that, unlike previously described assays, allows their identification in mixed populations of peripheral blood leukocytes as well as their immunophenotyping. Apoptotic lymphocytes are identified on the basis of peculiar light scatter changes, reflecting their smaller size and their modified nucleus/cytoplasm organization, and of the decreased expression of surface CD45 molecules. Based on these criteria, apoptotic lymphocytes generated by exposure to ionizing radiation can be
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Jørgensen, Louise Bruun, Terese L. Katzenstein, Jan Gerstoft, Lars R. Mathiesen, Court Pedersen, and Claus Nielsen. "Genotypic and Phenotypic Nevirapine Resistance Correlates with Virological Failure during Salvage Therapy Including Abacavir and Nevirapine." Antiviral Therapy 5, no. 3 (1999): 187–94. http://dx.doi.org/10.1177/135965350000500302.

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Objective To study the development of resistance during 8 weeks of salvage therapy with abacavir and nevirapine in combination with other reverse transcriptase inhibitors (RTIs) and protease inhibitors (PIs). Methods Samples obtained at baseline and after 8 weeks of therapy from 16 heavily pretreated patients were analysed for genotypic and phenotypic resistance. Genotypic resistance was analysed in cell-associated DNA and plasma HIV-RNA using direct sequencing. Phenotypic resistance was analysed in a PBMC-based assay and in a recombinant virus assay. Plasma viral load was measured at baseline
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Gasper, Melanie, David Sherman, and Donald Sodora. "Monocyte and NK cell dysfunctional responses to Mycobacteria in the context of chronic HIV-1 infection (P4377)." Journal of Immunology 190, no. 1_Supplement (2013): 183.24. http://dx.doi.org/10.4049/jimmunol.190.supp.183.24.

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Abstract Coinfection with M. tuberculosis (Mtb) and other Mycobacteria is a leading cause of morbity and mortality in HIV+ patients. While neither monocytes nor NK cells are directly infected with HIV, several of their phenotypic and functional properties become altered during chronic infection, and both cell types are important to the control of Mtb. We hypothesized that HIV-related NK cell dysfunction may result from impaired monocyte dysfunction and impaired crosstalk with NK cells, which we will evaluate in the context of Mycobacteria infection. In fact, we have observed a decrease in IL-1
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Dissertations / Theses on the topic "HIV, phenotypic assay, cell based assay"

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Giannini, Alessia. "HIV drug discovery and resistance testing through cell based assays." Doctoral thesis, Università di Siena, 2019. http://hdl.handle.net/11365/1069117.

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Anti-HIV therapy has dramatically progressed from palliative care to high effectiveness, with virus replication and disease progression halted in most patients yet not eradicated. However, the need for lifelong therapy has kept anti-HIV drug development at high pace and novel strategies, drugs and drug classes have been regularly introduced over time. Issues to be tackled during prolonged antiretroviral therapy include adherence, toxicity, drug-drug interactions and the development of drug resistance. During my PhD course at the HIV Monitoring Laboratory (HML) of the Department of Medical Biot
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Giammarino, Federica. "PHENOTYPIC CHARACTERIZATION OF NOVEL ANTIVIRALS FOR THE TREATMENT OF MULTIDRUG RESISTANT HIV-1 AND EMERGING VIRUSES." Doctoral thesis, Università di Siena, 2023. https://hdl.handle.net/11365/1224634.

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Abstract Phenotypic characterization of novel antivirals for the treatment of multidrug resistant HIV-1 and emerging viruses Doctoral Research School of Medical Biotechnologies – Cycle XXXV Supervisor: Maurizio Zazzi; Candidate: Federica Giammarino Background The need for new antiviral drugs has increased overtime due to the worldwide circulation of different viruses together with the increased frequency and diversity of new outbreaks. The ideal option for a prompt response against both emerging and re-emerging viruses is represented by the use and the development of direct acting antivi
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Dragoni, Filippo. "Antiviral drug development for treatment of acute and chronic viral infections." Doctoral thesis, Università di Siena, 2021. http://hdl.handle.net/11365/1127988.

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Background Viruses are aetiologic agents of several human infectious diseases, which can be distinguished into acute and chronic. Among the agents causing acute infections, arboviruses, such as Dengue (DENV), West Nile (WNV) and Zika (ZIKV) Viruses, have increasingly spread worldwide. Moreover, the newly discovered Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2), causing the COVID-19 disease, is responsible for an incredibly quantity of infections and deaths. Conversely, the Human Immunodeficiency Virus (HIV-1) is a well-known pathogen able to cause chronic latent infection, but
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Lai, Ming Chih, and 賴銘志. "Cloning of HIV-1 Integrase into a Moloney Murine Leukemia Virus Based Vector to Establish a Cell Line for In Vivo Integration Assay of HIV-1 Integrase." Thesis, 1994. http://ndltd.ncl.edu.tw/handle/48848916245915300130.

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Book chapters on the topic "HIV, phenotypic assay, cell based assay"

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Rajakuberan, Chitra, Brett J. Hilton, and Roland Wolkowicz. "Protocol for a Mammalian Cell-Based Assay for Monitoring the HIV-1 Protease Activity." In Methods in Molecular Biology. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-937-2_27.

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Liu, Zhaoping, Andrea Gomez-Donart, Caroline Weldon, Nina Senutovitch, and John O’Rourke. "Developing a Novel Multiplexed Immune Assay Platform to Screen Kinase Modulators of T Cell Activation." In High-Throughput Screening for Drug Discovery [Working Title]. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.97304.

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T cell activation plays a central role in inflammation, autoimmune diseases and cancer. Cancer immunotherapies, such as immune checkpoint inhibitor, bi-specific antibody, chimeric antigen receptor T (CAR T) cell, and adoptive tumor-infiltrating lymphocyte (TIL) therapies require the characterization and monitoring of T cell activation. Here we describe a novel, multiplex immune assay platform based on high-throughput flow cytometry technology and advanced computational algorithms for data analysis. The assay simultaneously measures T cell dynamics including phenotype, time-dependent expression of activation markers, secreted effector cytokines, and proliferation. The assay screened a kinase chemogenomic library and identified 25 kinase inhibitors with distinct inhibition profiles on early (CD69) and late (CD25) activation markers and the cytokines IFNγ and TNFα. We identified 5 kinase inhibitors with dissimilar effects on CD69 and CD25 expression, and a cluster of total 4 MEK1//2 inhibitors with similar activation profiles. The screening revealed 3 kinase inhibitors for PKC, IKK2, and MEK1/2 respectively, all with a phenotypic signature similar to ruxolitinib, a Jak1/2 inhibitor used to treat myelofibrosis disease. These results suggest this multiplexed assay platform, combined with a chemogenomic library screening, may be used as primary screen for phenotypic or target-based drug discovery, target identification, and potential drug repositioning.
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Conference papers on the topic "HIV, phenotypic assay, cell based assay"

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Gerckens, Michael, Katharina Heinzelmann, Oliver Eickelberg, and Gerald Burgstaller. "A phenotypic cell-based extracellular matrix deposition assay for target validation and drug discovery." In ERS International Congress 2017 abstracts. European Respiratory Society, 2017. http://dx.doi.org/10.1183/1393003.congress-2017.pa912.

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Chowdhury, Arnab Roy, Debabani Roy Chowdhury, Manoj Pandre, Samrat Roy, Sundarajan Kannan, and John W. Ellingboe. "Abstract 2868: A cell based phenotypic assay platform for cancer metastasis drug discovery and diagnostics." In Proceedings: AACR Annual Meeting 2021; April 10-15, 2021 and May 17-21, 2021; Philadelphia, PA. American Association for Cancer Research, 2021. http://dx.doi.org/10.1158/1538-7445.am2021-2868.

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Chan, Grace Ka-Yan, Tracy Kleinheinz, Matthew Martinson, Hok-Sum Cheung, and John G. Moffat. "Abstract 740: Phenotypic profiling and selectivity optimization of ERK inhibitors using a high-throughput imaged-based cell cycle assay." In Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CA. American Association for Cancer Research, 2014. http://dx.doi.org/10.1158/1538-7445.am2014-740.

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Wehrman, Tom S., Erika A. O'Donnell, M. Jared Lumpe, Benjamin E. Osetek, and Peter O. Krutzik. "Abstract 5385: A novel cell-based kinase assay panel paired with high content phenotypic profiling to correlate kinase inhibitor specificity and cellular activity." In Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CA. American Association for Cancer Research, 2014. http://dx.doi.org/10.1158/1538-7445.am2014-5385.

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Reports on the topic "HIV, phenotypic assay, cell based assay"

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Weil, Clifford F., Anne B. Britt, and Avraham Levy. Nonhomologous DNA End-Joining in Plants: Genes and Mechanisms. United States Department of Agriculture, 2001. http://dx.doi.org/10.32747/2001.7585194.bard.

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Repair of DNA breaks is an essential function in plant cells as well as a crucial step in addition of modified DNA to plant cells. In addition, our inability to introduce modified DNA to its appropriate locus in the plant genome remains an important hurdle in genetically engineering crop species.We have taken a combined forward and reverse genetics approach to examining DNA double strand break repair in plants, focusing primarily on nonhomologous DNA end-joining. The forward approach utilizes a gamma-plantlet assay (miniature plants that are metabolically active but do not undergo cell divisio
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