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1
Gray, Rodney. "The Hoax." Thesis, University of North Texas, 2013. https://digital.library.unt.edu/ark:/67531/metadc271822/.
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The HOAX is an examination of abusive power and lack of regulation in the homeowners' association industry; a business and quasi-government system whose key selling point is the protection of property values. The documentary follows an investigative reporter, homeowners, and HOA reform activists as they illustrate shocking evidence of financial and psychological hardships throughout Texas and Nevada. A few of these people, including the filmmaker, are the subject of adverse actions from various players in this quasi-governmental system.
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Dudink, Peter. "Hoax, Parody, and Conservatism in Harry Potter." Thesis, University of Waterloo, 2002. http://hdl.handle.net/10012/760.
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This essay examines the ideology or value system implicit in Joanne Rowling's Harry Potter series. Many of the images in the series, despite being fantastic or empirically unprecedented, are minor transformations of popular books and of our very common physical and cultural reality. However, these imaginative transformations of mundane reality actually imitate, reiterate, and conserve common and contemporary secular values. On a third level the thesis will show that this conservation of contemporary secular values is undermined by a cynical and very subtle transformative element of satire, parody, and criticism.
Depending on the theme explored by the particular chapter, a different level of meaning might be evident. Chapter One discusses Rowling's parody of popular secular values. Chapter Two focuses on her parody of Christianity. Chapter Three focuses on Rowling's representations of nature and technology and on her parodic reversal of their traditional representation in similar literature. Chapter Four discusses how Rowling has made a critical appropriation of popular culture's reliance on thoughtless and 'instant' solutions, and discusses how she has made a mockery of her own hero, Harry Potter.
The conclusion discusses the value of literary devices that transform literal meanings and verbal images into new meanings and images, and concludes that Harry Potter should be read cautiously. A second conclusion is that the author's claim the series is incomplete is a hoax. This argument is defended with a demonstration that the existing four Harry Potter books form a complete unit, and with a reminder that an element of hoax pervades Rowling's entire series.
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Patel, Kushang. "Automatic Hoax Detection on Social Media Using Deep Learning." Thesis, Blekinge Tekniska Högskola, Fakulteten för datavetenskaper, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:bth-21184.
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Conley, Timothy John. "The hoax that joke bilked, sense, nonsense, and Finnegans Wake." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp03/MQ29487.pdf.
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Conley, Tim. "The hoax that joke bilked : sense, nonsense, and Finnegans wake." Thesis, McGill University, 1997. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=26682.
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The remarkable challenges Finnegans Wake offers to its readers and to the very process of reading are the results of an evolution of Nonsense literature. Despite the unduly "serious" framework of criticism which has been built up around it, Joyce's anomalous last work is a radical "hoax" upon interpretation. The regular confluences of linguistic deconstruction (via word association as well as recurring word and phrase matrices) and ontological metaphor, developed from authors such as Rabelais, Sterne, and Lewis Carroll, are offered by the Wake as tests to the reader's (qua reader) sensibilities. As Nonsense, Finnegans Wake departs from typified modernist modus operandi (metonymic allusion) and instead explores the limits of metaphor. The stakes of Joyce's hoax are of vital interest to the contemporary student of literature and culture, since the Wake dares the reader to find new meanings rather than to project old ones; to exult its eccentricities and its difference; and all the while to call into question (as the text itself does), its authenticity and authority.
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Coccaro-Pons, Jennifer. "A Mixed Method Study of Prospective Teachers' Epistemic Beliefs and Web Evaluation Strategies Concerning Hoax Websites." FIU Digital Commons, 2018. https://digitalcommons.fiu.edu/etd/3911.
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Teachers need to be equipped with the tools necessary to evaluate content on the Internet and determine if it is a credible source, or a hoax website since they are expected to instruct and prepare students on how to evaluate the sites which is now a relevant phenomenon. The purpose of the mixed‑method study was to obtain an understanding of the web evaluation strategies of prospective teachers regarding the evaluation of hoax websites and how their epistemic beliefs may influence their evaluation. Another aspect of this study was to find out what outcomes resulted from providing guidance, or not to prospective teachers before evaluating the hoax websites.
Seventy‑two prospective teachers from undergraduate education courses completed an online questionnaire, where they evaluated four websites (two hoaxes and two credible) and completed questions regarding their epistemic beliefs. Two groups of prospective teachers were selected. Group A was the control group and Group B was the experiment group. Group A simply took the online questionnaire. However, Group B was provided with an overview of a specific web evaluation strategy, the WWWDOT Framework, before taking the online questionnaire. Sixteen participants were interviewed. Interestingly, almost half of the participants (48.6%), trusted at least one of the hoax websites.
The study concluded that teaching the WWWDOT Framework helped to increase the number of people that did not trust the aesthetically appealing hoax website in Group B. Regarding epistemic beliefs, prospective teachers, who displayed feeling‑based epistemic beliefs, tended to trust the hoax website that was aesthetically appealing in Group A. The qualitative results provided additional insights and supported the quantitative data. The qualitative research suggests that lateral reading, spending sufficient time to read and evaluate and knowing the definition of a hoax website as being the most important web evaluation strategies displayed by those that did not trust the hoax websites.
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Ahrenius, Malin. "”Russian hoax” eller ”Russian collusion”? : En kritisk diskursanalys kring utredningen av den ryska inblandningen i USA:s presidentval 2016." Thesis, Linnéuniversitetet, Institutionen för statsvetenskap (ST), 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-106855.
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The aim of this study is to analyse "Report On The Investigation Into Russian Inerference In The 2016 Presidential Election", or the "Mueller report", and William Barr's summary of the same report by using poststructural critical discourse analysis. This critical analysis aims to examine how their potrayal differ and to analyse what impact that might have had on the political discource surrounding the US Presidency, Russia and the US election. In conclusion they differ quite a bit. While the Mueller report show supstantial evidence of obstruction of justice by Donald Trump, William Barr found that the report completely exonerated him. Attorney General William Barr's summary of the report was the first summary of the report to reach the public. Perhaps his description of the report had an impact on the decision whether to impede Donald Trump after the report came out. What impact did the Mueller report and Barr's summary of the same have on the political discource surrounding the legitimacy of the US election and the US presidency?
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Hansson, Sandra. "Is Self-Service Business Intelligence a hoax? : A descriptive study of casual users’ independence using SSBI in the data mining process." Thesis, Linnéuniversitetet, Institutionen för informatik (IK), 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-106081.
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When using Business Intelligence (BI), organizations can improve decision-making bycompiling, understanding and utilizing the data held by the organization. Self-serviceBusiness Intelligence (SSBI) has emerged as a new focus within BI and aims to make BI tools available to business users, and relieve IT-experts involvement of in ad hoc reporting andanalysis. The aim of this research is to examine challenges of casual users being self-reliant when using SSBI tools, and the way they are using them in the data mining process. This wasdone through a qualitative study, interviewing seven individuals from different user groups: casual users, power user and IT-experts. From the results it appears that most casual users are not sufficiently self-reliant in the data mining process using SSBI. The visualization is theprime area for SSBI, which most casual users manage themselves if the data and dashboards are pre-defined for them. SSBI is becoming increasingly more common, which leads to moreand more casual users with increased experience who need to be able to dig out their own data for interpretation and analysis. Yet, without additional knowledge, such as data knowledge or SQL skill, casual users are in need of support when it comes to more complex operations thanad hoc analysis and reporting, still creating a request-response relationship to power users and IT-experts. The major challenges, limiting casual users from being self-reliant, are: not sufficiently user-friendly tools, poor data definition and lack of data knowledge, limited data access, indigent validation of reports and lastly, inadequate education.
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Säfwenberg, Nike Linn. "”Så nu är jag ett annat jag igen” : Autenticitetsgränser i och kring JT LeRoys Sarah och Hjärtat är bedrägligast av allt." Thesis, Södertörn University College, School of Gender, Culture and History, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:sh:diva-1697.
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The aim of this essay is to investigate how the author JT LeRoy (a. i. Laura Albert) questions and broadens the concept of authenticity in literature and authorship. My study is based on LeRoy’s novel Sarah [2000] and the collection of short stories The heart is deceitful above all things [2001], as well as articles written about the construction of Laura Albert’s alter ego JT LeRoy. I look for norms and boundaries in connection with authentic authors, identities, sex, gender and love. My method is that of a thematic analysis focusing on names, parenthood, religious beliefs and sub cultural norms and resistance. I am inspired by Michel Foucault’s thoughts on discourse, power and sexuality. My results are presented in a dialogue with previous readings and queer theory, foremost represented by Judith Butler. My general conclusion is that the literary texts, as well as the author represent a queer perspective, and that they therefore – in a heteronormative world view – are considered neither normal nor authentic.
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Harder, Erik E. "Don't Believe Everything You Read: Hoaxes and Satire in The Narrative of Arthur Gordon Pym." Cleveland State University / OhioLINK, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=csu1408389819.
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Gattolin, André. "L'acte canularesque médiatique : dispositifs, procédés et enjeux communicationnels (Europe et Amérique du Nord, 2004-2008)." Thesis, Paris 3, 2011. http://www.theses.fr/2011PA030056/document.
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Cette thèse porte sur la recrudescence notable depuis une décennie de certains phénomènes singuliers de mystification mettant en jeu des dispositifs communicationnels assez élaborés, connus sous l’appellation de canulars médiatiques. Apparu au XVIIIe siècle et en filiation étroite avec certaines pratiques populaires plus anciennes, l’acte canularesque associé à l’usage des médias engage la mise en œuvre de procédés audacieux qui, au fil du temps et de l’émergence de nouveaux moyens de communication, n’ont cessé de se sophistiquer.L'analyse d'un corpus de neuf canulars ayant recueilli d'un fort retentissement médiatique au cours de la période 2004-2008 souligne l’importance du jeu des interactions qui s’établissent entre l’auteur, sa cible et le public exposé.Elle témoigne également de la forte incidence du contexte sur la réussite de l’acte. Les transformations profondes qui traversent actuellement la société à l’échelle internationale, ainsi que les importants bouleversements qui modifient et parfois fragilisent le monde de l’information, représentent à l’évidence un terrain propice à la multiplication des canulars médiatiques. Entre la précarisation des conditions d’exercice du journalisme, la concurrence effrénée qui affecte les médias, l’attrait grandissant pour les fictions se donnant pour vraies et la recherche permanente de l’inédit et du spectaculaire, les fausses nouvelles et autres mystifications malicieuses trouvent toute raison de proliférer.La tournure très transgressive prise par les canulars médiatiques au cours de ces dernières années a conduit récemment à la mise en place de législations plus coercitives et entraîné de la part de leurs victimes des mesures de rétorsion qui nous amènent, en dernière partie de cette thèse, à nous interroger sur le devenir incertain de l’objet et de sa pratiqueThis thesis deals with the issue of a decade-long rise in the number of particular phenomena of mystification that use very elaborate communication mechanisms : the media hoaxes. Appeared in the 18th century and closely linked to ancient and popular practices, the elaborate hoax, associate with media and new technologies, implements audacious processes that became increasingly sophisticated. The analysis of nine hoaxes cases that have benefited from a wide media echoe during the period 2004 to 2008 emphasizes the significance of interaction between the author, his target and the audience. It also testifies the context's high impact upon the success of the action. The profound transformations that go through worldwide societies and the upheavals that modify and often weaken the information world obviously represent a favourable ground for a multiplication of media hoaxes. From the erosion of conditions in journalism practices, the fiercecompetition which affects various media, the growing attraction for fictions as true, to the constant search for novelty and the spectacular, false information and other mischievous mystifications find every reason to proliferate.As the transgressive course taken by media hoaxes in recent years has led to a stringent legislation and the victims' retaliations, we question the uncertain future of this research topic as a practice in the final part of the thesis
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Rossi, Robert. "Léo Taxil : [1854-1917] : du journalisme anticlérical à la mystification transcendante." Thesis, Aix-Marseille, 2014. http://www.theses.fr/2014AIXM3063.
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Dans le contexte politique national si paradoxal de République monarchiste [1871-1879], la presse satirique radicale marseillaise est essentiellement animée par une poignée de jeunes journalistes à l'humour corrosif qui s'assignent pour mission de s'attaquer aux moeurs et aux vices de leur temps. De cette équipe, une personnalité controversée se détache : Léo Taxil, personnage à la conscience « accommodante », pris dès l'adolescence dans une sorte de fuite en avant et une volonté d'indépendance, dues à la nécessité de très tôt devoir gagner sa vie et qui côtoie les grands courants de pensée de son époque, se rangeant tour à tour dans les deux camps profondément antagonistes de la période. Sa conversion au catholicisme et la mystification dont il s'est rendu coupable à travers l'affaire Diana Vaughan ont fait couler beaucoup d'encre et focalisé l'attention de ses contemporains, puis des chercheurs. Pourtant, si de nombreux ouvrages évoquent le parcours atypique de Léo Taxil pour tenter d'expliquer son formidable canular, ils passent de façon succincte sur des épisodes marquants de son existence. Or, qu'en a-t-il été de la vie même de Taxil ? De ses premiers combats exaltés, feints ou réels, de son anticléricalisme militant, associé à des activités lucratives, éléments qui préfigurent son invraisemblable conversion au catholicisme et ses révélations sur la « franc-maçonnerie luciférienne » auxquelles une partie non négligeable du clergé s'est ralliée ? En quoi ce polémiste marseillais est-il révélateur des problèmes de son temps, complètement investi dans les luttes féroces que se livrent républicains laïcs et catholiques conservateurs ?In such a paradoxical national political context of a monarchist Republic [1871-1879], the radical satirical press of Marseille is essentially run by a handful of young journalists with a sharp sense of humour who set themselves the task of tackling the custom and vices of their time. Out of this team, a controversial figure stood out : Léo Taxil. Manipulator, with an "accommodating" conscience, desperate to achieve his aims, caught as early as a teenager in a kind of headlong rush with a desire of independence due to the very early need to earn a living, he met the great currents of thought of his time, and unscrupulously and seemingly without a second thought, alternately sided with two strongly antagonistic camps. His conversion to Catholicism and his hoax in the Diana Vaughan case were much written about, and were the focus of attention for his contemporaries and later for researchers. Yet, if these works evoke the atypical path of Leo Taxil in order to try and explain his tremendous hoax, they succinctly go over significant events in his life. But beyond the very much narrated and commented hoax, what about Taxil's very life? What about his early enthusiastic battles, whether feigned or real, what about his militant anticlericalism, coupled with lucrative activities, elements that prefigure his improbable conversion to catholicism and his revelations about the "Luciferian Freemasonry" eventually joined by a significant part of the clergy? In what way is this Marseille polemicist indicative of the problems of his time, fully invested in the fierce battles between non religious Republicans and conservative Catholics ?
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Čepelka, Michal. "Formáty lži." Master's thesis, Vysoké učení technické v Brně. Fakulta výtvarných umění, 2018. http://www.nusl.cz/ntk/nusl-377163.
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I perceive the thesis as a result of long-term considerations of forms of lies and manipulation and their importance in today's society. Based on the research of these forms and the environments in which they occur, I try to detect the effect of the lie on both individuals and society. And with the use of multiple media that capture this exploration process.
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Zhou, Bo. "Structural studies of geminin-hox and smad-hox complexes /." View abstract or full-text, 2007. http://library.ust.hk/cgi/db/thesis.pl?BICH%202007%20ZHOU.
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Bahnert, Thomas [Verfasser]. "Entwicklung eines HOAI-konformen Skalierungssystems des Leistungsbildes Gebäude und Innenräume nach § 34 HOAI zur Anwendung für BIM : Development of a HOAI-compliant scaling system for buildings and interior spaces according to §34 HOAI for use in BIM / Thomas Bahnert." Hamburg : Universitätsbibliothek der HafenCity Universität Hamburg (HCU), 2021. http://nbn-resolving.de/urn:nbn:de:gbv:1373-repos-7014.
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O'Neill, C. M. "HOX gene expression in haematopoiesis : investigation of the role of HOX A7." Thesis, Queen's University Belfast, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.368469.
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Weber, Christine. "Cdx-Hox protein interactions." Thesis, University of Ottawa (Canada), 2008. http://hdl.handle.net/10393/27656.
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Cdx and Hox gene families both code for homeodomain-containing transcription factors and both are required for proper patterning of the anterior-posterior axis. Although Cdx is known to be upstream of certain Hox genes, preliminary data indicated Cdx1 interacts in vitro with Hoxd4 but not Hoxa9, and that this interaction is localized to the C-terminal region of both proteins. GST-pulldown assays with in vitro radiolabelled proteins or transfected cell lysates were used to investigate the extent of the Cdx-Hox interactions. The interaction was narrowed down to specific residues in the first half of the homeodomain, but an inhibitory N-terminus can abrogate interaction in vitro. A larger group of Hox proteins interact with GST-Cdx when they are expressed in a cellular environment. This could mean that the interaction requires other cofactors, such as Hox cofactors Pbx and Meis that also interact with GST-Cdx1 in vitro. This study is the first example of Cdx-Hox protein interactions and suggests that the Cdx-Hox complex could be involved in the regulation of downstream Hox target genes.
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Lyon, Raymond Scemama Jean-Luc. "Hox Gene Expression During Oreochromis niloticus Pharyngeal Arch Development: Discovering the Hox Code." [Greenville, N.C.] : East Carolina University, 2010. http://hdl.handle.net/10342/2728.
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De, Kumar Bony. "Induction of Hox genes and genome wide identification of Hox binding sites in mice." Thesis, Open University, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.607467.
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Hox genes encode a family of transcription factors that play highly conserved regulatory roles in specifying the properties of tissues in developing embryos. Very little is known about how HOX proteins control the cellular and developmental processes governing morphogenesis through regulation of down-stream target genes. The goal of this research was to investigate on a genome-wide basis, the rules and principles which underlie the binding of different HOX proteins to target sites and understand the basis for their distinct specificities. I utilized the programmed differentiation of mouse embryonic stem cells into a neural fate with retinoids and genomic technologies to systematically investigate binding properties of two HOX proteins, HOXA1 and HOXBI and their cofactors PBX and MEIS. I analyzed the induction properties of the cells and the transcriptional dynamics and epigenetic states in Hox clusters to explore the differentiation process. An extensive and dynamic pattern of transcriptional activity indicates that Hox clusters generate a large number of non-coding RNAs which may impact their activation and chromatin states. Global identification of HOXB 1, HOXA1, PBX and MEIS binding regions by chromatin immune precipitation and high throughout sequencing (ChIP-seq) has generated insight into many potential Hox target genes. HOXA1 binding peaks generally overlapped with those of PBX and MEIS, supporting their roles as HOX co-factors. The sites bound by HOXBl uncovered new classes of binding motifs. Regulatory assays demonstrated that many of these novel motifs functioned as neuronal enhancers. Many HOXB I binding peaks have closely associated REST motifs and bind the REST repressor complex, which is important in neuronal differentiation. The close association of REST and HOXB 1 binding sites provides a mechanism for coordinating cell differentiation programs in neurogenesis. This research has uncovered novel properties of HO X proteins and their co-factors that underlie their role as master regulators of patterning and morphogenesis
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Gédéon, Laurent. "Les Hoa au Viet-Nam." Paris 8, 1999. http://www.theses.fr/1999PA081709.
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Les chinois du vietnam (appeles hoa) occupent une place particuliere dans ce pays pour plusieurs raisons : l'anciennete de leur implantation, les relations historiques millenaires entre la chine et le vietnam, leur organisation sociale tres poussee, leur puissance economique, et le fait qu'ils ont ete les principales victimes de la crise qui a eclate entre les deux etats en 1978. Celle-ci, qui les a durablement affectes, est intervenue dans un contexte regional de tension avec la chine, et d'opposition croissante entre leur communaute, tres influente economiquement, et les nouvelles autorites du vietnam reunifie, decidees a transformer l'economie de l'ancien sud-vietnam. Elle a eu pour effet l'exode de centaines de milliers de hoa hors du pays. Elle represente aujourd'hui pour eux l'evenement de reference de leur histoire recente, dont les consequences conditionnent encore nombre de leurs choix personnels, sociaux, et economiques. Plus de vingt ans apres, les chinois ont regagne une partie de leur influence ; ils constituent un ensemble homogene, dont la cohesion est entretenue par la transmission d'un heritage culturel et linguistique commun. La majorite d'entre eux se regroupe aujourd'hui dans le sud du vietnam, surtout a ho-chi-minh ville, particulierement dans les 10eme, 11eme, 6eme, et surtout 5eme arrondissements de l'agglomeration, qui forment le quartier de cholon et ou ils sont commercialement tres actifs. Mais ils demeurent un groupe allogene et faiblement assimile, socialement et linguistiquement distinct, en butte aux critiques d'une partie de la population en raison de leur particularisme et de leur emprise economique, jugee trop importante. Une telle situation pose la question de leur devenir, qui apparait par ailleurs etroitement lie aux questions geopolitiques regionales, notamment l'evolution des relations sino-vietnamiennes.
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Ronshaugen, Matthew Rand. "Evolution of Hox protein function /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2002. http://wwwlib.umi.com/cr/ucsd/fullcit?p3071026.
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Valerius, Michael Todd. "Downstream effectors of the homeobox transcription factor Hoxa 11." Cincinnati, Ohio : University of Cincinnati, 2004. http://www.ohiolink.edu/etd/view.cgi?acc%5Fnum=ucin1078859617.
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Thesis (Ph. D.)--University of Cincinnati, 2004.Title from electronic thesis title page (viewed July 14, 2006). Includes abstract. Keywords: homeobox; vertebrate; microarray; development. Includes bibliographical references.
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Lincoln, Joy. "Developmental studies of the murine homeobox gene, Hoxa-9." Thesis, Durham University, 2002. http://etheses.dur.ac.uk/4145/.
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Cell patterning during embryogenesis is essential for establishing the identity of the developing body plan. Hox genes are fundamental regulators of tissue organisation along the anterior-posterior body axis of the developing embryo. These homeodomain-containing proteins act as transcription factors during normal development. The function of the homeodomain is to bind sequence-specific DNAmotifs which allows either activation or repression of downstream effector genes, which consequently results in the control of tissue-specific determination and differentiation. Aberrant expression of such Hox genes, including Hoxa-9 can result in homeotic transformations leading to phenotypic malformations and oncogenesis. However the normal function of Hoxa-9 is poorly understood. This study explored the potential role for Hoxa-9 in normal development and differentiation. An in situ hybridisation approach was taken to define the expression of Hoxa-9 in the developing mouse. Hoxa-9 was found to expressed in a temporarily and spatially regulated manner, in particular being detected in the developing cardiac atria, ventricles and cardiac vessels during E9.5-E12 stages of development. The expression of this homeotic gene during in vitro differentiation of embryonic stem cells into cardiomyocytes and haematopoietic cells demonstrated a profile that correlated with the emergence of these cell types. The functioning relationship between Hoxa-9 expression and lineage commitment was Airther explored using over-expression in embryonic stem cells. A potential role for Hoxa-9 in normal development is discussed.
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Bussiere, Marianne. "Characterising the MLL complex : epigenetic regulation of Hoxa genes." Thesis, University of Birmingham, 2010. http://etheses.bham.ac.uk//id/eprint/707/.
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The Mixed-Lineage Leukaemia (MLL1) protein is a key developmental factor that acts to regulate genes via its histone methyl-transferase activity. This study aimed to examine how MLL1 and its associated complex contribute to gene regulation in a functionally relevant cell background. To assess dynamic processes involved, we developed a haematopoietic Stem Cell (hSC) -based system, in which Hoxa genes are down-regulated in a differentiation- induced manner. I characterised the histone modification distribution on two MLL-target genes showing the largest changes upon differentiation: Hoxa4 and Hoxa5. When active, these genes are associated with a peak of “activating” histone marks (H3K4me3, H3K9ac, H4K16ac) over the transcriptional start site, which are lost when the gene is repressed. This correlated with the recruitment of enzymes that deposit these marks, including components of the MLL complex (MLLC, MLLN and menin) as well as HATs that may be associated with the complex (CBP and MOF). Interestingly, the location of these proteins does not always correlate with the marks they deposit. We show that the dual mark H3K9acS10p is present on active Hoxa4 and Hoxa5 genes, and correlates with the presence of the histone kinase Msk1. We speculate that Msk1 contributes to regulating MLL1 HMT’ase activity on these genes.
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Sessa, Luca. "Epigenetic control of human HOX clusters." Thesis, Open University, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.402692.
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Kelly, Zoe L. "HOX gene expression in ovarian cancer." Thesis, University of Surrey, 2015. http://epubs.surrey.ac.uk/808411/.
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Ovarian cancer is the leading cause of cancer death among all gynaecological cancers. Its aggressive nature is partly due to genetic heterogeneity and the lack of effective treatments strategies. Standard treatment involves cytoreductive surgery followed by chemotherapy using a platinum-based agent. Although initially, patients response well to this treatment, the majority will relapse and develop recurrent disease, predominantly due to the emergence of platinum resistance. Further understanding of the molecular changes which occur during ovarian ontogenesis and in the development of platinum resistance is essential to design new targeted drugs to improve patient prognosis. HOX gene are a family of homeodomain-containing transcription factors that determine cell and tissue identity in the early embryo and are found to be aberrantly expressed in cancer. HOX gene expression in ovarian cancer of different histological subtypes and in primary ovarian tumours were evaluated here. This is the first comprehensive study of HOX gene expression in a cohort of primary ovarian tumours, including statistical analysis of HOX gene expression profiles along with clinic-pathological data of each patient. HOX genes were found to be profoundly dysregulated in ovarian cancer cell lines and primary ovarian tumours, with very little to no expression found in normal ovarian and fallopian tube tissue. A 5-HOX gene signature which predicts poor overall survival in ovarian cancer patients was identified. Platinum resistant disease displayed an overall higher level of HOX gene expression, significantly HOXB4 and HOXB9. This dysregulated HOX expression reported could therefore act as a set of targets for therapeutic intervention. The novel peptide, HXR9, has been developed to block the interaction between HOX proteins and their co-factor, PBX, and therefore subsequent target gene expression. The efficacy of HXR9 treatment of ovarian cancer cells was explored. HXR9 treatment was shown to induce cell death via apoptosis in ovarian cancer cells shown by an increase in apoptotic cells identified by flow cytometric analysis, and increase in caspase-3 activity and the upregulation of pro-apoptotic gene cFos. Enhanced cell cytotoxicity was observed when combining HXR9 with cisplatin to treat platinum resistant cells, revealing a new therapeutic option for drug resistant disease that should be explored further for potential clinical trial investigation. A limiting factor towards the development of new treatment strategies is the lack of a reliable animal model of ovarian cancer. Common methods used to test new drugs involve in vitro investigation and the use of engineered animal models. However, these models do not represent the true heterogeneity and complexity of human ovarian tumours. Therefore, the use of the chicken chorioallontoic membrane (CAM) as a model of ovarian cancer for the testing of anti-cancer drugs was assessed. Cell lines grafted onto the CAM successfully and developing into micro-tumours. Cell cultured from ascites samples of ovarian cancer patients also grafted, but with a less success rate. Morphological and tumour retardation was detected after treatment with HXR9. This demonstrated the potential of this model to be developed for future personalised drug screening.
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Ackema, K. B. "Hox genes and mesenchymal stem cells." [S.l.] : Rotterdam : [The Author] ; Erasmus University [Host], 2008.
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Svingen, Terje, and n/a. "Hox Transcription Factors: Their Involvement in Human Cancer Cells and In Vitro Functional Specificity." Griffith University. School of Biomolecular and Biomedical Science, 2005. http://www4.gu.edu.au:8080/adt-root/public/adt-QGU20050830.135356.
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Hox genes are regulatory genes encoding small proteins containing a highly conserved 61-amino acid motif, the homeodomain, that enables Hox proteins to bind to DNA at specifically recognised binding sites and transcriptionally activate their target genes. In mammalian species there are 39 Hox genes and they are structural and functional homologs of the Drosophila homeotic complex (Horn-C). During embryogenesis and early development the Hox genes are expressed in a spatiotemporal fashion, where they operate as master transcriptional regulators. Hox genes are further expressed in fully differentiated adult cells, potentially in a tissue-specific manner involving maintenance of the normal phenotype. In selected oncogenic transformations, dysregulated Hox gene expression has been observed, indicating an involvement of these transcriptional regulators in carcinogenesis and metastasis. Utilising quantitative real-time PCR assays, these studies investigated the expression patterns of 20 Hox genes and two wellcharacterised Hox cofactors (Pbx and Meis) in malignant and non-malignant human breast and skin cancer cells. Dysregulated Hox expression was observed for all malignancies tested, of which some misexpressed Hox genes seemed random, whereas other Hox transcripts showed altered levels potentially corresponding with the invasive capacity of the cells. Also, the Hox cofactors Pbx and Meis showed no marked changes in expression levels from the non-malignant to the malignant phenotypes, indicating that it is dysregulated Hox gene expression rather than dysregulated gene expression of Hox cofactors that potentially commit the cell to redifferentiate and undergo oncogenic transformation. Although the Hox proteins are known to be key transcriptional regulators of development, the mechanisms by which they gain their in vivo functional specificity is still largely unknown. They all show strikingly similar transcriptional specificity in vitro, yet show unique specificity in their in vivo environment. This paradox has been the subject of intense scrutiny, however very few direct Hox target genes have been identified, making it a difficult task to decipher the exact manner in which Hox proteins exert their functional potential. Therefore, the studies presented herein were aimed at identifying further Hox target genes in the human system. Utilising differential display approaches, several potential downstream target genes were isolated. Substantiated with real-time PCR assays, one of these potential targets was selected as a likely direct Hox gene target, and as such subjected to further studies. By the combination of bioinformatic analyses, transfection protocols and luciferase assays, a gene encoding the SR-related protein SRrpl3O was shown to be trans-activated in vitro by HOXD4 via a putative Hox binding element within its promoter region. This is the first reported link between Hox transcription factors and the SR and SR-related family of pre-mRNA splicing proteins, offering a new and exciting insight into the complex nature of Hox functional specificity. Finally, this thesis also puts forward new ideas regarding how the Hox proteins gain their transcriptional and functional specificity. Utilising bioinformatic tools in conjunction with performing an extensive review of the disparate catalogue of Hox-related research reports, work herein offers the first comprehensive analysis of the mammalian Hox gene targets in relation to their promoter structures, as well as with respect to the expanded Hox DNA-binding elements. This work reports that identified Hox targets generally contain TATA-less core promoters, many of which have several GC-box elements. The Hox binding elements show no apparent preference regarding their location relative to the transcription start site (TSS), as they are found both upstream and downstream of the TSS, as well as being located close to proximal core promoter elements for some genes and at more distant positions in other gene promoters. Finally, the core Hox binding element TAAT/ATTA contains only part of the necessary recognition sequence involved in Hox-DNA binding, and the notion that flanking base pairs dictate trans-regulatory potential is further explored with the hypothesis that the immediate 3' base pair dictates an activator/repressor-switch of the Hox trans-regulatory effect.
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Senate, University of Arizona Faculty. "Faculty Senate Minutes April 1, 2013." University of Arizona Faculty Senate (Tucson, AZ), 2013. http://hdl.handle.net/10150/288664.
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Kettyle, Laura Margaret Joy. "Criticality of the Hoxa cluster in novel models of leukaemia." Thesis, Queen's University Belfast, 2015. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.695386.
Full textAbstract:
Class I Homeobox genes (Hox) are an evolutionary conserved family of genes that encode master regulators in developmental processes. Hox gene expression has been shown to be high in normal haematopoietic stem/progenitor cells (HSPCs) and decreases during normal differentiation, however elevated Hox expression remains in subtypes of leukaemia. Polycomb repressor complexes (PRCs) and histone modifiers, e.g. Mixed Lineage Leukaemia (MLL), are key regulators of Hox expression. MLL rearrangements, frequent in acute leukaemia, are associated with high HOXA expression. However, the necessity of the Hoxa cluster in MLL-Ieukaemia maintenance is not fully elucidated. Ectopic overexpression of MLL-AF9 in HSPCs in conditional compound transgenic mouse backgrounds MxCre+/Hoxafloxlflox (MAFF) or Hoxafloxlflox (Aflox) models resulted in increased colony formation and growth in liquid culture. Transformed colonies, serially replated in methylcellulose and transplanted into sub-lethally irradiated recipient mice, resulted in primary leukaemia. Direct treatment of MLL-AF9 leukaemias generated in the MAFF background (MAFF-MA9) with interferon-alpha (IFN)resulted in further deletion of the Hoxa cluster and significant reduction in colony formation compared to controls. Although non-leukaemic MAFF HSPCs retained colony forming ability after complete Hoxa cluster deletion (Hoxa-/-), no Hoxa-/- colonies were recovered from the IFN treated MAFF-MA9 cultures. Cre-recombinase induced deletion of the Hoxa cluster from Aflox-MA9 leukaemia cells was confirmed by gDNA-PCR and sequencing. Transplantation of Cre-treated Aflox-MA9 cells resulted in significant increased survival (p
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Jerković, Ivana. "Systematic Analysis of Posterior HOXA/HOXD Function in Mesenchymal Cells." Doctoral thesis, Humboldt-Universität zu Berlin, 2018. http://dx.doi.org/10.18452/19467.
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HOX-Gene sind essentielle Transkriptionsfaktoren (TFs), die den Körperplan, die Struktur und die Organbildung während der Entwicklung bestimmen. Diese komplexen Prozesse werden präzise von in verschachtelter Weise exprimierten HOX-Genen reguliert. In vitro Experimente zeigten
jedoch, dass die HOX-DNA-Bindungsdomäne stark konserviert ist und oft ähnliche DNA-Sequenzen bindet. Die niedrige biochemische Bindungsspezifität und die hochspezifischen Funktionen stehen oft im Widerspruch und bilden das Schlussthema des so genannten Hox-Paradoxons. Das Paradox besteht aufgrund der folgenden Hindernisse: hohe Proteinhomologie, unspezifischen Antikörper sowie die verschachtelte HOX-Expressionsmuster. Das Ziel dieser Arbeit war, diese Probleme zu
überwinden, die HOX-DNA-Bindung in kontrollierten und physiologischen Umstände zu untersuchen und die Bindung von neun Gliedmaßen-spezifischen posterioren HOXA und -D-TFs zu vergleichen. Zu diesem Zweck wurden neun Hühner-HOX-Gene (HOXA- und HOXD9-13) mit dem FLAG markiert und mittels Viren in Gliedmaßen-mesenchymalen Zellen exprimiert. Somit wurde der Vergleich unter identischen und kontrollierten Bedingungen ermöglicht. Im Einklang mit in vivo Funktionsdaten zeigten die HOX-Bindungsprofile, dass zwei direkte Paraloge (z. B. HOXA10 und D10) häufiger dieselben Regionen binden als zwei Nicht-Paraloge (z. B. HOXA9 und A13). Außerdem, die hier beschriebene HOX-DNA-Bindung unterscheidet sich von in vitro Bindung, was darauf hinweist, dass Kofaktoren für deren biologische Funktion wichtig sind. Zusätzlich ergab sich aus dem Bindungsvergleich, dass es zuvor unbekannte Unterschiede zwischen Bindungsweise von HOX-TFs gibt, die zumindest teilweise auf der Häufigkeit von direkter Bindung und Ko-Bindung mit anderen TFs beruhen. Schließlich wurde mit der Kombination von Genetik, Genomik und Biochemie einen neuen HOX-Kofaktor entdeckt, CTCF, der auf ein mögliches Wechselspiel zwischen der HOX-Zielregulation und der Chromatinarchitektur hindeutet.HOX genes are essential developmental transcription factors (TFs) that pattern the animal body plan, their structures and organs. To precisely control these very diverse processes HOX genes are expressed in a nested fashion and regulate their targets in a context specific way. However, in vitro experiments indicated that HOX DNA binding domain (Homeodomain) is remarkably rigid and often binds very similar DNA sequences. This discrepancy between high functional specificity and low in vitro biochemical specificity is at the core of a problem termed Hox paradox. This paradox persists due to several biological and technical obstacles; namely high HOX protein homology and lack of sufficiently specific antibodies as well as nested HOX expression pattern. The aim of this study was to address these problems, study HOX-DNA binding in a controlled, Hox-native environment and to compare HOX-DNA binding of nine posterior vertebrate HOXA and HOXD TFs. To do this, nine chicken HOX genes (HOXA9-13 and HOXD9-13) were FLAG-tagged and virally expressed in chicken mesenchymal limb-derived cells enabling comparison of their binding in an identical setup and controlled conditions. HOX binding profiles uncovered two direct paralogues (i.e. HOXA10 and D10) bind more often same regions than two non-paralogues (i.e. HOXA9 and A13) reminiscent of in vivo functional data. Moreover, the here described in vivo HOX-DNA binding differs from in vitro binding, indicating the importance of cofactors and biological context for HOX binding and functional outcome. Additionally, binding comparison uncovered previously unknown differences between binding modes of HOX-TFs that at least partially rely on the abundance of direct binding and co-binding with other TFs. Finally, with combination of genetics, genomics and biochemistry a novel HOX cofactor, CCCTC binding factor (CTCF), was discovered suggesting potential interplay between HOX target regulation and chromatin architecture.
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Smith, Margaret Louise. "An analysis of Hox genes in Myriapods." Thesis, University of Cambridge, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.624853.
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Aspland, Simon Eric. "Extradenticle and HOX gene function in Drosophila." Thesis, University of Cambridge, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.627253.
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Veraksa, Alexey Nikolaevich. "Modulators of Hox protein function in Drosophila /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2000. http://wwwlib.umi.com/cr/ucsd/fullcit?p9975051.
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Litim-Mecheri, Isma. "Spécificité des protéines Hox : Rôle des motifs connus et découverte de nouveaux motifs." Thesis, Aix-Marseille 2, 2011. http://www.theses.fr/2011AIX22096.
Full textAbstract:
Les gènes Hox sont responsables de l’identité des segments le long de l’axe antéro-postérieur. Ils sont évolutivement conservés et codent des facteurs de transcription. In vitro, toutes les protéines Hox se lient à des séquences nucléotidiques très similaires via un domaine de liaison à l’ADN très conservé, l’homéodomaine (HD). Cette faible spécificité de liaison à l’ADN in vitro contraste avec leur spécificité d’action in vivo. Une manière d’expliquer ce paradoxe est que les protéines Hox agissent en interaction avec des protéines cofacteurs, dont le mieux caractérisé est Extradenticle (Exd) chez la drosophile, Pbx chez les mammifères (collectivement appelés PBC). L’interaction Hox-PBC s’appui sur un motif conservé chez la presque totalité des protéines Hox, situé en amont de l’HD, le motif Hexapeptide (HX). Des travaux récents au sein de notre équipe ont montré l’existence d’un nouveau mode d’interaction Hox-PBC, non-générique, médié par un motif spécifique à certains groupes de paralogies seulement, et situé en en C-terminal de l’HD. Ceci souligne que les interactions Hox-PBC, qui spécifient la fonction des protéines Hox, reposent sur de modes multiples d’interaction.Mes travaux de thèse ont porté sur le mode d’action des protéines Hox, en étudiant la fonction de trois motifs ayant un rôle démontré ou potentiel dans le recrutement du cofacteur Exd par la protéine Hox de drosophile AbdominalA (AbdA). L’approche empruntée visait à analyser de manière globale la manière dont chacun de ces motifs pris isolément, ou en interaction, définit la fonction d’AbdA. Les conclusions de ce travail soulignent l’absence de pléiotropie fonctionnelle et un haut degré d’interactivité entre ces motifs. Le second volet de ma thèse a été d’initier la découverte de nouveaux motifs fonctionnels au sein des protéines Hox. J’ai abordé cette question en sélectionnant des modules phylogénétiquement conservés. Afin d’évaluer leur fonction, ces motifs ont été mutés et l’impact de leur mutation a été analysé in vitro et in vivo. Les résultats obtenus ont permis l’identification d’au moins un domaine protéique qui contribue de manière prédominante à la fonction de la protéine DfdHox genes are responsible for the identity of segments along the antero-posterior axis. They are evolutionarily conserved and encode transcription factors. In vitro, all Hox proteins bind to a similar nucleotide sequence via a highly conserved DNA binding domain, the homeodomain (HD). This low specificity of DNA binding in vitro contrasts with their specificity in vivo. One way to explain this paradaox is that Hox protein function with protein cofactors, best represented by Extradenticle (Exd) in Drosophila, Pbx in mammals (collectivaly refered as PBC). Hox-PBC interaction relies on a motif located upstream of the HD, conserved in most Hox proteins, the Hexapeptide (HX). Recent work in our group identified a novel mode of Hox-PBC interaction, non-generic, specific to a subset only of Hox paralog groups, and relying on a motif located C-terminal to the HD. This highlight plasticity in Hox-PBC interaction.My PhD work aimed at investigating the mode of action of Hox protein, by studying the function of three protein motifs, with known or putative role in Exd recruitment by the Drosophila Hox protein AbdominalA (AbdA). The approach taken aimed at analyzing, using a large functional window, how these motifs, taken in isolation or collectively, define AbdA protein activity. Conclusions highlight the absence of pleitropy and a high degree of functional interaction for these protein motifs. The second part of my PhD work has been to initiate the search for novel functionally important protein motifs within Hox proteins. This was approached by selecting phylogenetically conserved motifs, and addressing their function in vitro and in vivo following motif mutations. At least one functional domain was isolated, that contributes predominantly to Dfd protein function
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Yang, Xiu. "Altered neuronal lineages in the facial ganglia of Hoxa₂ mutant mice." Cleveland, Ohio : Case Western Reserve University, 2008. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=case1207189742.
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Vu, Hoan [Verfasser]. "Rolled-up plasmonic metamaterials coupled to quantum-well emitters / Hoan Vu." München : Verlag Dr. Hut, 2018. http://d-nb.info/1170473350/34.
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Kirkbride, Helen J. "Expression of the HOXA gene cluster in human myeloid cell development." Thesis, University of Newcastle Upon Tyne, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.242454.
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VALERIUS, MICHAEL TODD. "DOWNSTREAM EFFECTORS OF THE HOMEOBOX TRANSCRIPTION FACTOR HOXA 11." University of Cincinnati / OhioLINK, 2004. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1078859617.
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Vũ, Nguyên Mạnh Hoan [Verfasser]. "Rolled-up plasmonic metamaterials coupled to quantum-well emitters / Hoan Vu." München : Verlag Dr. Hut, 2018. http://d-nb.info/1170473350/34.
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Nguyen, Hoai Viet [Verfasser]. "Authentication in Ultra Large Scale REST-based Systems / Hoai Viet Nguyen." Hamburg : Staats- und Universitätsbibliothek Hamburg Carl von Ossietzky, 2020. http://d-nb.info/1224048873/34.
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Plaça, Jessica Rodrigues. "Avaliação do perfil genômico dos genes da família HOX em tumores a partir de dados de bancos públicos." Universidade de São Paulo, 2017. http://www.teses.usp.br/teses/disponiveis/17/17154/tde-17042018-161612/.
Full textAbstract:
A família de genes HOX compreende um conjunto de fatores de transcrição altamente conservados evolutivamente. Em mamíferos, os genes HOX se subdividem em 4 clusters: HOXA, HOXB, HOXC e HOXD, atuando no desenvolvimento embrionário com a regulação de processos biológicos como proliferação, diferenciação, migração, angiogênese e apoptose que são reativados durante a carcinogênese. Estudos recentes apontam que os genes HOX podem exercer papel relevante na formação de diversos tumores sólidos, todavia ainda não foi possível caracterizar sistematicamente a expressão dos genes HOX em tumores bem como determinar seus alvos em tumores. Desta forma, o objetivo geral deste trabalho consistiu na caracterização in silico do modelo de atuação genes HOX na carcinogênese. Para cumprir este objetivo foi identificado o perfil diferencial dos genes HOX entre amostras normais e tumorais. Alvos de genes HOX foram identificados e, quando diferencialmente expressos, foram associados com os genes HOX, independentemente dos índices de metilação e CNA. Por fim, as associações finais entre os genes HOX e seus alvos foram enriquecidas com os bancos de dados KEGG e GO. Identificou-se diferentes assinaturas de expressão de genes HOX em diferentes tumores, associadas com o eixo ântero-posterior do corpo humano, bem como os folhetos embrionários originários aos tecidos tumorais, compatível com o padrão de expressão no desenvolvimento embrionário. Um número considerável de genes HOX atuam preferencialmente via enhancers na regulação de seus alvos. Como exemplo, os genes HOXB7 e HOXC11, que funcionam como moduladores anti tumorais. Finalmente, o estudo mostra que diante do número crescente de dados genômicos públicos, é possível viabilizar projetos de grande valor científico.The HOX gene family comprises a set of evolutionarily highly conserved transcription factors. In mammals, HOX genes are subdivided into four clusters: HOXA, HOXB, HOXC and HOXD, acting on the embryonic development with regulation of biological processes such as proliferation, differentiation, migration, angiogenesis and apoptosis that are reactivated during carcinogenesis. Recent studies indicate that HOX genes may play a relevant role in the formation of several solid tumors, but it has not been possible to systematically characterize the expression of HOX genes in tumors as well as to determine their targets in tumors. Thus, the general aim of this project was to characterize the in vivo model of HOX genes in carcinogenesis. To accomplish this goal the differential profile of HOX genes was identified between normal and tumor samples. HOX gene targets were identified and, when differentially expressed, were associated with HOX genes regardless of methylation and CNA indices. Finally, the final associations between the HOX genes and their targets were enriched with the KEGG and GO databases. Different signatures of HOX gene expression were identified in different tumors, associated with the anteroposterior axis of the human body, as well as the embryonic leaflets originating from the tumor tissues, compatible with the expression pattern in the embryonic development. A considerable number of HOX genes preferentially act via enhancers in the regulation of their targets. As an example, the HOXB7 and HOXC11 genes, which function as pro-tumor modulators. Finally, the study shows that in view of the growing number of public genomic data, it is possible to make feasible projects of great scientific value.
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Abdel, Samad Omar. "Hox genes and the specification of neuronal phenotype in the vertebrate hindbrain : transcriptional regulation of the Hox target gene Phox2b." Université Louis Pasteur (Strasbourg) (1971-2008), 2004. http://www.theses.fr/2004STR13027.
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Caronia, Giuliana. "A 147L substitution in the HOXD13 homeodomain causes a novel human limb malformation by producing a selective loss of function." Thesis, Open University, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.275109.
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Goh, Siew-Lee. "Functional studies of MEIS1, a HOX co-factor." Thesis, McGill University, 2007. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=103200.
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HOX proteins are evolutionarily conserved homeodomain-containing transcription factors involved in hematopoiesis and patterning during embryogenesis. Their tasks as master regulators of embryonic development are achieved in large part through their ability to interact with co-factors of the PBX and MEIS/PREP families, which constitute the broader three amino-acid loop extension (TALE) class of homeodomain proteins. HOX, MEIS, and PBX have been implicated in leukemic hematopoiesis due to their association with hematological malignancies. The oncogenic function of MEIS1 in accelerating the onset of acute myeloid leukemia induced by HOX was mapped to its C-terminal transactivation domain, which is responsive to PKA signaling. This thesis extends our understanding regarding the mechanism by which MEIS1A executes its C-terminal transactivation function in vivo. We describe the involvement of CREB and its co-activators CBP and TORC in conferring the PKA-responsiveness of the ME1S1A C terminus. CREB mutants that fail to bind CBP or TORC also fail to promote PKA induction mediated by the C terminus of ME1S1A. TORC was further shown to be capable of bypassing the need for PKA to activate transcription by MEIS1, an ability endowed by its physical interaction with MEIS1. Chromatin immunoprecipitation (ChIP) demonstrated a concerted recruitment of endogenous MEIS1, TORC2, and CREB proteins on ME1S1 target genes. In addition, this thesis also characterizes the promoter of the murine Meis1 gene. Meis1 possesses multiple transcription start sites upstream of its translation initiation site. We identified a ME1S·PBX consensus recognition site within the Meis1 promoter and showed that PBX1 binds to this sequence in vitro. Our ChIP assay results further suggest an autoregulatory mode for the Meis1 gene as revealed by a co-occupancy of endogenous CREB, TORC2, PBX1, and MEIS1 itself on the Meis1 promoter. Collectively, this thesis proposes a mechanistic action conferred by CREB, CBP and TORC in the PKA-inducible transactivation of ME1S1A, and provides new information on the Meis1 promoter.
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Averof, Michalis. "HOM/Hox genes of a crustacean : evolutionary implications." Thesis, University of Cambridge, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.319512.
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Sharpe, D. J. "HOX gene expression in head and neck cancer." Thesis, Queen's University Belfast, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.517517.
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Elstob, Philip Ronald. "Hox gene function and cell identity in Drosphila." Thesis, University College London (University of London), 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.272353.
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Tümpel, Stefan Wolfgang. "Transcriptional regulation of Hox genes during hindbrain development." Thesis, Open University, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.424272.
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Di, Rocco Giuliana. "Functional characterisation of a HOX/PBX transcriptional complex." Thesis, Open University, 1998. http://oro.open.ac.uk/57746/.
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Hox genes control cell fates and specify regional identities during vertebrate development. Hox proteins relaxed DNA-binding selectivity in vitro, suggests that Hox specificity of action is achieved in vivo through the action of transcriptional cofactors. Members of the EXD/PBX family of homeoproteins have been proposed for such a role on the basis of genetic and biochemical evidence. In this work we show that the human Pbxl and HOXBl proteins can cooperatively activate transcription through a genetically charaterized Hox responsive element, the autoregulatory enhancer of the mouse Hoxb-T gene (b-lARE), which directs the spatially restricted expression of Hoxb-L in the fourth rhombomere during hindbrain development. On the b-lARE, only a restricted subset of HOX proteins is able to bind cooperatively and activate transcription. Selective recognition of the b-lARE is mediated by the N-terminal region of the HOX homeodomain. The DNA binding and protein-protein interaction functions of HOXBl and Pbxl are all necessary for the assembly of a transcriptionally active complex on the b-lARE. Functional dissection of the complex allowed the localization of the main activation domain in the HOXBl N-terminal region, and of an additional one in the C-terminal region of Pbxl, which is absent in one of its two alternative splicing isoforms. The transcriptional activity of HOX/Pbxl complexes on the b-lARE element is maximal and further restricted in embryonal carcinoma (EC) cells compared to other cell lines. Mutational analysis shows that an octamer-binding protein consensus site on the b-lARE contributes to the activity of the enhancer in transfected cells and it is bound in vitro by octamer-like proteins from both EC cells and embryonic extracts.