Relevant bibliographies by topics / Holographic microscopy

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers

Academic literature on the topic 'Holographic microscopy'

Author: Grafiati
Published: 4 June 2021
Last updated: 17 June 2025

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Journal articles on the topic "Holographic microscopy"

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Fedorov, A. G., V. V. Platonov, L. L. Zhondorova, and L. N. Fedorova. "Development of a digital holographic microscope model for the investigate of structures in the optical range." Vestnik of North-Eastern Federal University History Political Science Law 21, no. 2 (2024): 77–83. http://dx.doi.org/10.25587/2222-5404-2024-21-2-77-83.

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One of the modern and relevant methods for investigating the structures of objects is based on the holographic method of recording signals (holographic microscopy). The main advantage of this method is the ability to obtain complete information about the object. In other words, this method makes it possible to record not only the amplitude, but also the phase of the wave. This is achieved thanks to a recording scheme in which the phase of the wave is some modulation of the intensity. This advantage makes holographic microscopy an effective tool for the investigate of particles/microparticles in gases, liquids and solid materials in the form of thin films or in sufficiently transparent materials for optical waves (one of the main limitations of the holographic recording scheme is to investigate only objects with high transmissivity, i.e. the reference wave is must be about 70% or more of the total wave). Within the framework of this work, we consider the scheme of in-line holography (Gabor holography). The undoubted advantage of the in-line holographic investigation method is that it is limited only by the wavelength range. In other words, by changing the wavelength of the source, a wide range of objects can be examined. For example, in-line holography is used in low energy electron microscopes, which allows the atomic structure of an object to be studied. In the case when the source is a laser (optical range), a holographic microscope provides a wide range of possibilities for investigation the micro-objects, from various bacteria to various fine–structured particles. We developed a model of a digital holographic microscope for the study of structures in the optical range, based on the Gabor in-line holography method. This model of the microscope is developed on the Raspberry Pi Zero 2W platform.
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Chen, Duofang, Lin Wang, Xixin Luo, Hui Xie, and Xueli Chen. "Resolution and Contrast Enhancement for Lensless Digital Holographic Microscopy and Its Application in Biomedicine." Photonics 9, no. 5 (2022): 358. http://dx.doi.org/10.3390/photonics9050358.

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An important imaging technique in biomedicine, the conventional optical microscopy relies on relatively complicated and bulky lens and alignment mechanics. Based on the Gabor holography, the lensless digital holographic microscopy has the advantages of light weight and low cost. It has developed rapidly and received attention in many fields. However, the finite pixel size at the sensor plane limits the spatial resolution. In this study, we first review the principle of lensless digital holography, then go over some methods to improve image contrast and discuss the methods to enhance the image resolution of the lensless holographic image. Moreover, the applications of lensless digital holographic microscopy in biomedicine are reviewed. Finally, we look forward to the future development and prospect of lensless digital holographic technology.
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Zhang, Tong, Ichirou Yamaguchi, and Hywel Morgan. "Digital Holographic Microscopy." Microscopy and Microanalysis 5, S2 (1999): 362–63. http://dx.doi.org/10.1017/s1431927600015130.

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We applied phase-shifting digital holography to microscopy in this paper. At first lensless microscopy is proposed, in which no optical adjustment is necessary. Then, the method is applied to relax the limitation of focal depth in traditional optical microscopy. A theory for image formation and experimental verification using a few specimens are described.keywords: microscopy, digital holography, phase shiftingDue to the finite focal depth of an imaging lens, a limitation to normal optical microscopy-is that, only the 2-dimensional (2-D) information of an object can be obtained at one time. Besides, it is not convenient for quantitative analysis the observed image. Optical sectioning microscopy (OSM) and scanning confocal microscopy (SCM) which use opto-electronic detection have been proposed for quantitative analysis of a 3-D object. However, the former requires critical mechanical adjustment, while the latter uses timeconsuming mechanical 3-D scanning. Holographic microscopy can solve these problems because it can record 3-D information at one time. But, the chemical processing of holograms and the mechanical focusing at the reconstructed images cause more or less trouble. A 3-D imaging technique without use of photographic recording called optical scanning holography has recently been reported. However, there are also some trouble owing to the twin-image noise.
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Balasubramani, Vinoth, Małgorzata Kujawińska, Cédric Allier, et al. "Roadmap on Digital Holography-Based Quantitative Phase Imaging." Journal of Imaging 7, no. 12 (2021): 252. http://dx.doi.org/10.3390/jimaging7120252.

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Quantitative Phase Imaging (QPI) provides unique means for the imaging of biological or technical microstructures, merging beneficial features identified with microscopy, interferometry, holography, and numerical computations. This roadmap article reviews several digital holography-based QPI approaches developed by prominent research groups. It also briefly discusses the present and future perspectives of 2D and 3D QPI research based on digital holographic microscopy, holographic tomography, and their applications.
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Yang, Shuntao. "Digital holographic microscopy of highly sensitive living cells." Journal of Computational Methods in Sciences and Engineering 21, no. 6 (2021): 1985–97. http://dx.doi.org/10.3233/jcm215504.

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In order to solve the problem that the existing living cell microscopy technology can not display the detailed information of cells, a high sensitivity digital holographic living cell microscopy technology is proposed in this paper. By measuring the phase distribution and refractive index distribution of living cells, the data of living cells are extracted and converted into digital hologram of living cells. Simulation and comparison of the commonly used two-dimensional living cell microscope methods. The experimental results show that the high-sensitivity digital holographic microscopic detection method can obtain the detailed information of living cells, which proves the effectiveness of this study.
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Yang, Thomas Zhirui, and Yumin Wu. "Seeing cells without a lens: Compact 3D digital lensless holographic microscopy for wide-field imaging." Theoretical and Natural Science 12, no. 1 (2023): 61–72. http://dx.doi.org/10.54254/2753-8818/12/20230434.

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Optical microscopy is an essential tool for biomedical discoveries and cell diagnosis at micro- to nano-scales. However, conventional microscopes rely on lenses to record 2-D images of samples, which limits in-depth inspection of large volumes of cells. This research project implements a novel 3-D lensless microscopic imaging system that achieves a wide field of view, high resolution, and an extremely compact, cost-effective design: the Digital Lensless Holographic Microscope (DLHM).A lensless holographic microscope is built with only a light source, a sample, and an imaging chip (with other non-essential supporting structures). The entire setup costs $500 to $600. A series of MATLAB-based algorithms were designed to reconstruct phase information of samples simultaneously from the recorded hologram with built-in high-resolution and phase unwrapping functions. This produces 3-D images of cell samples. The 3-D cell reconstruction of biological samples maintained a comparable resolution with conventional optical microscopes while covering a field of view of 36.2 mm2, which is 20-30 times larger. While most microscopes are extremely time-consuming and require professional expertise, the lensless holographic microscope is portable, low-cost, high-stability, and extremely simple. This makes it accessible for point-of-care testing (POCT) to a broader coverage, including developing regions with limited medical facilities.
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Dudek, Julia, Mikołaj Rogalski, Julianna Winnik, Piotr Arcab, Piotr Zdańkowski, and Maciej Trusiak. "Autofocusing method for lensless digital in-line holographic microscopy with misaligned illumination." Photonics Letters of Poland 16, no. 4 (2024): 79–81. https://doi.org/10.4302/plp.v16i4.1306.

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This study presents a correction method in Lensless Digital In-line Holographic Microscopy accounting for tilted illumination to address challenges caused by misalignments in the optical setup. An autofocusing method is discussed, utilizing a sharpness criterion based on amplitude variance to find both propagation distance and illumination tilt for precise holographic reconstruction of phase objects. The proposed algorithm was rigorously tested under large illumination angles, demonstrating its effectiveness in maintaining high reconstruction quality for demanding imaging scenarios. Full Text: PDF References Z. Huang and L. Cao, "Deep learning sheds new light on non-orthogonal optical multiplexing", Light Sci Appl 13, 145 (2024). CrossRef V. Balasubramani, M. Kujawińska, C. Allier, V. Anand, C.-J. Cheng, C. Depeursinge, N. Hai, S. Juodkazis, J. Kalkman, A. Kuś, M. Lee, P.J. Magistretti, P. Marquet, S.H. Ng, J. Rosen, Y.K. Park, and M. Ziemczonok, "Roadmap on Digital Holography-Based Quantitative Phase Imaging", J. Imaging 7, 252 (2021). CrossRef V. Anand, T. Katkus, D.P. Linklater, E.P. Ivanova, S. Juodkazis, "Lensless Three-Dimensional Quantitative Phase Imaging Using Phase Retrieval Algorithm", J. Imaging 6, 99 (2020). CrossRef A. Ozcan, E. McLeod, "Lensless Imaging and Sensing", Annual Review of Biomedical Engineering 18, 77 (2016). CrossRef C.J. Potter, Z. Xiong, E. McLeod, "Clinical and Biomedical Applications of Lensless Holographic Microscopy", Laser Phot. Rev. 18, 2400197 (2024). CrossRef N.N. Evtikhiev, S.N. Starikov, P.A. Cheryomkhin, V.V. Krasnov, V.G. Rodin, "Numerical and optical reconstruction of digital off-axis Fresnel holograms", Proc. SPIE 8429, 84291M (2012). CrossRef T. Wu, Y. Yang, H. Wang, H. Chen, H. Zhu, J. Yu, X. Wang, "Investigation of an Improved Angular Spectrum Method Based on Holography", Photonics 11, 16 (2024). CrossRef M. Trusiak, J.-A. Picazo-Bueno, P. Zdankowski, V. Micó, "DarkFocus: numerical autofocusing in digital in-line holographic microscopy using variance of computational dark-field gradient", Optics and Lasers in Engineering 134, 106195 (2020). CrossRef F. Dubois, C. Schockaert, N. Callens, C. Yourassowsky, "Focus plane detection criteria in digital holography microscopy by amplitude analysis", Opt. Express 14, 5895 (2006). CrossRef Y. Zhang, H. Wang, Y. Wu, M. Tamamitsu, A. Ozcan, "Edge sparsity criterion for robust holographic autofocusing", Opt. Lett. 42, 3824 (2017). CrossRef Z. Ren, Z. Xu, E.Y. Lam, "Learning-based nonparametric autofocusing for digital holography", Optica 5, 337 (2018). CrossRef R.W. Gerchberg, W.O. Saxton, "A Practical Algorithm for the Determination of Phase from Image and Diffraction Plane Pictures", Optik 35, 237 (1972). CrossRef J.R. Fienup, "Phase retrieval algorithms: a comparison", Appl. Opt. 21, 2758 (1982). CrossRef T.-C. Poon, T. Kim, G. Indebetouw, B.W. Schilling, M.H. Wu, K. Shinoda, Y. Suzuki, "Twin-image elimination experiments for three-dimensional images in optical scanning holography", Opt. Lett. 25, 215 (2000). CrossRef Y. Rivenson, Y. Wu, H. Wang, Y. Zhang, A. Feizi, A. Ozcan, "Sparsity-based multi-height phase recovery in holographic microscopy", Sci. Rep. 6, 37862 (2016). CrossRef M. Rogalski, P. Arcab, L. Stanaszek, V. Micó, C. Zuo, M. Trusiak, "Physics-driven universal twin-image removal network for digital in-line holographic microscopy", Opt. Express 32, 742 (2024). CrossRef Y. Rivenson, Y. Wu, A. Ozcan, "Deep learning in holography and coherent imaging", Light Sci. Appl. 8, 85 (2019). CrossRef
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Nienałtowski, Patryk, Maria Baczewska, and Małgorzata Kujawińska. "Comparison of fixed and living biological cells parameters investigated with digital holographic microscope." Photonics Letters of Poland 12, no. 1 (2020): 13. http://dx.doi.org/10.4302/plp.v12i1.971.

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The statistical analysis and comparison of biophysical parameters of living and fixed, mouse embryonic fibroblasts cells are presented. The parameters are calculated based on phase measurements performed by means of a digital, holographic microscope. The phases are retrieved from off-axis, image plane holograms, followed by custom image segmentation and statistical analysis of cells’ surface, phase volume and dry mass. The results indicated statistically significant differences between fixed and living cell parameters, which is an important message for setting methodology for further diagnosis based on quantitative phase (label-free) analysis.Full Text: PDF References:K. Alm, et al. "Cells and Holograms – Holograms and Digital Holographic Microscopy as a Tool to Study the Morphology of Living Cells", InTech, 2013. [CrossRef]Y. Rivenson, Y. Wu, A. Ozcan, Light: "Deep learning in holography and coherent imaging", Science & Applications, 8, Art. No. 85 (2019) [CrossRef]Min, et al. Optics Letters, 42, Issue 2, pp. 227-230, (2017) [CrossRef]M. Baczewska, Measurements and analysis of cells and histological skin sections based on digital holographic microscopy, WUT master thesis, 2018. [CrossRef]P. Stępień, D. Korbuszewski, M. Kujawińska, "Digital Holographic Microscopy with extended field of view using tool for generic image stitching", ETRI Journal, 41(1), 73-83, (2019). [CrossRef]S. Beucher, Serge, The Watershed Transformation Applied To Image Segmentation, Scanning microscopy. Supplement 6, (2000) [DirectLink]J. A. Hartigan, M. A. Wong, "A K-Means Clustering Algorithm", Applied Statistics, (1979) [CrossRef]J. Serra, Image Analysis and Mathematical Morphology, Academic Press, (1982) [DirectLink]P. Girshovitz, N. T. Shaked, "Generalized cell morphological parameters based on interferometric phase microscopy and their application to cell life cycle characterization", Biomedical Optics Express Vol. 3, Issue 8, pp. 1757-1773, (2012) [CrossRef]
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Li, Ying, Wenlong Shao, Lijie Hou, and Changxi Xue. "Phase Disturbance Compensation for Quantitative Imaging in Off-Axis Digital Holographic Microscopy." Photonics 12, no. 4 (2025): 345. https://doi.org/10.3390/photonics12040345.

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Holographic detection technology has found extensive applications in biomedical imaging, surface profilometry, vibration monitoring, and defect inspection due to its unique phase detection capability. However, the accuracy of quantitative holographic phase imaging is significantly affected by the interference from direct current and twin image terms. Traditional methods, such as multi-exposure phase shifting and off-axis holography, have been employed to mitigate these interferences. While off-axis holography separates spectral components by introducing a tilted reference beam, it inevitably induces phase disturbances that compromise measurement accuracy. This study provides a computational explanation for the incomplete phase compensation issue in existing algorithms and establishes precision criteria for phase compensation based on theoretical formulations. We propose two novel phase compensation methods—the non-iterative compensation approach and the multi-iteration compensation technique. The principles and applicable conditions of these methods are thoroughly elucidated, and their superiority is demonstrated through comparative experiments. The results indicate that the proposed methods effectively compensate for phase disturbances induced by the tilted reference beam, offering enhanced precision and reliability in quantitative holographic phase measurements.
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Kemper, Björn, Patrik Langehanenberg, and Gert von Bally. "Digital Holographic Microscopy." Optik & Photonik 2, no. 2 (2007): 41–44. http://dx.doi.org/10.1002/opph.201190249.

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Dissertations / Theses on the topic "Holographic microscopy"

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El, Mallahi Ahmed. "Automated 3D object analysis by digital holographic microscopy." Doctoral thesis, Universite Libre de Bruxelles, 2013. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/209489.

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The main objective of this thesis is the development of new processing techniques for digital holograms. The present work is part of the HoloFlow project that intends to integrate the DHM technology for the monitoring of water quality. Different tools for an automated analysis of digital holograms have been developed to detect, refocus and classify particles in continuous fluid flows. A detailed study of the refocusing criterion permits to determine its dependencies and to quantify its robustness. An automated detection procedure has been developed to determine automatically the 3D positions of organisms flowing in the experiment volume. Two detection techniques are proposed: a usual method based on a global threshold and a new robust and generic method based on propagation matrices, allowing to considerably increase the amount of detected organisms (up to 95 %) and the reliability of the detection. To handle the case of aggregates of particles commonly encountered when working with large concentrations, a new separation procedure, based on a complete analysis of the evolution of the focus planes, has been proposed. This method allows the separation aggregates up to an overlapping area of around 80 %. These processing tools have been used to classify organisms where the use of the full interferometric information of species enables high classifier performances to be reached (higher than 93 %).<br>Doctorat en Sciences de l'ingénieur<br>info:eu-repo/semantics/nonPublished
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Degani, Ismail. "Biomedical applications of holographic microscopy." Thesis, Massachusetts Institute of Technology, 2018. http://hdl.handle.net/1721.1/118494.

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Thesis: S.M. in Engineering and Management, Massachusetts Institute of Technology, System Design and Management Program, 2018.<br>This electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections.<br>Cataloged from student-submitted PDF version of thesis.<br>Includes bibliographical references (pages 77-79).<br>Identifying patients with aggressive cancers is a major healthcare challenge in resource-limited settings such as sub-Saharan Africa. Holographic imaging techniques have been shown to perform diagnostic screening at low cost in order to meet this clinical need, however the computational and logistical challenges involved in deploying such systems are manifold. This thesis aims to make two specific contributions to the field of point-of-care diagnostics. First, it documents the design and construction of low-cost holographic imaging hardware which can serve as a template for future research and development. Second, it presents a novel deep-learning architecture that can potentially lower the computational burden of digital holography by replacing existing image reconstruction methods. We demonstrate the effectiveness of the algorithm by reconstructing biological samples and quantifying their structural similarity relative to spatial deconvolution methods. The approaches explored in this work could enable a standalone holographic platform that is capable of efficiently performing diagnostic screening at the point of care.<br>by Ismail Degani.<br>S.M. in Engineering and Management
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Bolognesi, Guido. "Optical studies of micron-scale flows : holographic microscopy, optical trapping and superhydrophobicity." Phd thesis, Université Claude Bernard - Lyon I, 2012. http://tel.archives-ouvertes.fr/tel-00870942.

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Microfluidics is a very recent branch of science and technology. The development and the success, it has had in the last 15 years, is mainly due to the concept of lab-on-a-chip. Those miniaturized devices, integrating one or more laboratory functions, have aroused great interest among several research areas as physics, chemistry, biology and bioengineering. When a fluid is confined in a micro or nano scale structure, its behaviour is strongly affected by its interactions with the surrounding surfaces. In this context, the theme of fluid/solid slippage has been widely studied both theoretically and experimentally. Innovative technologies to enhance the surface slippage by specifically designing the solid interfaces have reportedly demonstrated to be an effective way to reduce the fluid/solid friction. To this end, superhydrophobic surfaces have increasingly attracted the interest of the scientific and technological community thanks to the large wall-slippage they present for liquid water. Though their behaviour has been extensively investigated through several theoretical and numerical methods, the experimental approaches are still indispensable to test and understand the properties of these surfaces. However, the lack of a general predicting model is also due to the fact that no one of the several existing experimental techniques has shown up as a very reliable one. Indeed, the reported measurements of slippage still depends on the specific adopted method, thwarting attempts to corroborate the proposed theoretical and numerical schemes. Therefore, it is evident that a more sensitive and effective experimental technique is still missing. This thesis began and developed inside the wider project of setting up an innovative technique to investigate the fluid-solid slippage on superhydrophobic surfaces by means of optical tweezers. Even though this project is still going on, this work reports the steps performed along the long way towards this main goal and it consists of a collection of several researches involving different scientific fields as optics, microscopy, surface science, microhydrodynamics, microfluidics and microfabrication. The researches presented in this work can be separated in two main categories: i) holographic micromanipulation and microscopy, ii) superhydrophobicity.
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Makarchuk, Stanislaw. "Measurement of cell adhesion forces by holographic microscopy." Thesis, Strasbourg, 2016. http://www.theses.fr/2016STRAE034/document.

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Les forces mécaniques, générées par la cellule jouent un rôle crucial dans l'adhésion cellulaire, qui est un processus commun à un grand nombre de lignées cellulaires. Afin de mesurer la champ des forces pendant l'adhérence cellulaire, nous utilisons la microscopie de force de traction, où la cellule adhère à la surface plane d'un substrat souple dans le plan. Les forces sont calculées à partir du champ de déplacement mesuré à l'intérieur du substrat sous la cellule. Nous avons construit le microscope, dans lequel nous utilisons des billes sphériques en polystyrène pour mesurer le champ de déplacement. Les positions des marqueurs sont obtenues en analysant I' image interférentielle des particules. Avec cette technique, nous atteignons une précision nanométrique sur le champ de déplacement des particules, ce qui nous permet d'améliorer la résolution en force de ce type de microscope. Les premières mesures ont été effectuées avec la lignée de cellules cancéreuses SW 480<br>Mechanical forces, generated by the cell plays crucial role in cell adhesion - common process for different cell lines. ln order to measure the force map during cellular adhesion, we use Traction Force Microscopy (TFM), where cell adheres to the soft substrate in 20 plane, and the forces are calculated from measured displacement field inside the substrate underneath the cell. We built the microscope, where instead of using fluorescent markers, we use spherical polystyrene beads in order to measure the displacement field. Positions of the markers are obtained by analyzing the interference pattern caused by the beads in bright-field light. With this technique, we reach nanometer accuracy of the microsphere position determination, that, respectively, influence accuracy of the calculated force field. With the microscope first measurements were performed with cancer cell line SW 480
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Flewellen, James Lewis. "Digital holographic microscopy for three-dimensional studies of bacteria." Thesis, University of Oxford, 2012. http://ora.ox.ac.uk/objects/uuid:94ff344b-51ec-41c5-a5f8-c579e16dccd7.

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Holography has the ability to render three-dimensional information of a recorded scene by capturing both the amplitude and phase of light incident on the recording medium. The application of digital camera technology and high-speed computing means digital holograms can be analysed numerically and novel applications can be found for this technology. This thesis explores the potential for both inline and off-axis digital holographic microscopy to study the three-dimensional swimming behaviour of bacteria. A high-magnification (225x) digital holographic microscope was designed and constructed with the ability to switch easily between inline and off-axis imaging modalities. Hardware aspects, in particular the illumination source, the choice of camera and data transfer rates, were considered. Novel strategies for off-axis holography combining dark field microscopy were designed and implemented. The localisation accuracy of the inline imaging modality was assessed by studying samples of polystyrene microspheres. The microscope is sensitive to stage drift on the order of angstroms per second and can successfully localise microspheres in dilute suspensions at least 100&mu;m from the objective specimen plane. As a simple test of the capabilities of the microscope, the diffusion coefficient of a 0.5&mu;m microsphere was found to be isotropic and consistent with the theoretical value. Amplitude and phase image reconstructions from the off-axis modality are demonstrated. High-magnification dark field off-axis holographic microscopy is shown to be superior to inline microscopy in localising 100nm gold nanoparticles. An artifact from our method of dark-field imaging, however, restricts the depth range to 15&mu;m. A lower-magnification (45x) configuration of the microscope was used to study the 3D swimming behaviour of wild type Escherichia coli as a qualitative demonstration of the potential for this instrument in microbiological applications.
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Lopez, Marcio André Prieto Aparicio. "Microscopia holográfica digital aplicada na análise de tecidos biológicos." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/43/43134/tde-23032013-124944/.

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Este trabalho teve como objetivo a aplicação do Microscópio Holográfico Digital para análise de amostras biológicas, por meio de imagens de parâmetros físicos e informação quantitativa de uma amostra, gerados através de hologramas digitais, o que não ocorre na holografia clássica. O processamento e análise dos hologramas digitais foi efetuada por um programa escrito por meio do software MatLab, empregando o método de Dupla Propagação. São explicados outros métodos para tratamento de hologramas digitais, presentes no programa. O método de Dupla Propagação foi discutido, destacando suas vantagens frente aos outros métodos. Foi aplicado o método de Volkov para a retirada de ambiguidade de fase. O processo de montagem do Microscópio Holográfico Digital foi descrito, por apresentar modificações em relação ao protótipo inicial adotado. Sete amostras foram analisadas no Microscópio Holográfico Digital, três de calibração e quatro para análise - sangue e solução concentrada de proteína denominada Beta2 Glicoproteína tipo I, ou Beta2-GPI. Para calibração, foram realizados testes de formação de imagem, realizando comparação em quatro microscópios descritos e explicados em funcionamento e princípio envolvidos na formação de imagens, utilizando a mesma amostra; e verificação das dimensões de uma amostra, por meio de medição usando ferramentas disponíveis no programa. Uma amostra de sangue de um indivíduo heterozigoto para Hemoglobina S (anemia falciforme) e uma amostra de sangue de um indivíduo homozigoto para hemoglobina A1 (controle normal) foram empregadas na forma de filmes líquidos secos sobre lâminas de vidro (extensão sanguínea). O uso de fixação foi avaliado com a amostra controle. Foram geradas imagens em duas e três dimensões para as amostras biológicas, reproduzindo as estruturas morfológicas de cada. Para a proteína Beta2-GPI, a análise envolveu somente imagens, sem extração de valores; apesar disso, os resultados mostraram possibilidades de aplicações em estudos futuros. Grandezas físicas foram calculadas para dois dos componentes sanguíneos (Plasma e Eritrócito), mostrando valores próximos daqueles conhecidos anteriormente. Entretanto, alguns valores foram considerados estimativas novas, por não se conhecer, até o momento, nenhum cálculo efetuado anteriormente. A análise comprovou a formação de imagens e a capacidade de mensuração oferecida pelo aparelho. Devido ao parâmetro da fase, foi possível extrair informações em três dimensões.<br>This work aimed the implementation of the Digital Holographic Microscope for the analysis of biological samples, using physical parameters images and quantitative data from a sample, both generated through digital holograms, which does not occur in Classical holography. Processing and analysis of holograms were performed by a program written using the MatLab software, applying the Double Propagation method. Other methods for the treatment of digital holograms were explained. The Double Propagation method was discussed, highlighting their advantages over other methods. The method of Volkov was applied for removing phase ambiguity. The Digital Holographic Microscope assembly process was described, because of the modifications made to the initial prototype adopted. Seven samples were analyzed in the digital holographic microscope, three of them for calibration and the other to the analysis - blood and a concentrated solution of a protein called type I Beta2 Glycoprotein, or Beta2-GPI. Calibration tests were made by observing and comparing four image microscopes, described and explained in operation and principles involved in the formation of images, using the same testing sample; and checking the dimensions of another sample through measurement, using digital tools available in the program. Hb S heterozygous (Sickle Cell disease) and Hb A1 homozygous (Control) blood samples were prepared in microscope slide glasses. Images were acquired in two and three dimensions for biological samples, reproducing their morphological structures. For Beta2-GPI, the analysis involved only images, and no values were extracted; nevertheless, the results showed potential applications in future studies. Physical quantities were calculated for two blood components (Plasma and Erythrocyte), showing values closer to those previously known. However, some values were considered new estimates, because there is no knowledge of any calculation made previously, until now, using Digital Holographic Microscopy. The analysis proved the formation of images and the measurement capacity offered by the apparatus. Due to the phase parameter, we were able to extract information in three dimensions.
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Martinez, Marrades Ariadna. "3D microscopy by holographic localization of Brownian metallic nanoparticles." Thesis, Paris 6, 2015. http://www.theses.fr/2015PA066018/document.

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Nous présentons une nouvelle technique de microscopie stochastique basée sur un montage d'Holographie Digitale pour l'imagerie des distributions d'intensité optique. Nous montrons comment cette technique de champ lointain peut être adaptée afin d'obtenir des images de superrésolution ainsi que de champ proche. En pratique, nous imageons des nanoparticules métalliques en mouvement Brownien dans un liquide, que nous localisons ensuite dans le but de contourner la limite de diffraction. Le mouvement aléatoire des particules nous permet une exploration complète de l'échantillon. Au-delà de la simple localisation, ces marqueurs métalliques agissent comme des sondes locales du champ électromagnétique, pouvant notamment diffuser la lumière confinée vers le champ lointain. Les possibilités de cette nouvelle technique sont illustrées à travers l'imagerie de l'intensité optique d'une onde évanescente et d'une onde propagative. Grâce à des méthodes de calcul très performantes, nous sommes capables de localiser des centaines de particules par minute, avec une précision de l'ordre de 3×3×10 nm3 pour des particules immobiles. En plus de l'imagerie des distributions de champ optique, nous présentons une application combinant nos mesures superrésolues et des mesures d'électrochimie pour l'étude des processus d'oxydation de nanoparticules d'argent à proximité d'une électrode. Nos résultats ouvrent la voie à une nouvelle technique d'imagerie superrésolue, particulièrement bien adaptée à la caractérisation optique dans des milieux liquides (comme des systèmes microfluidiques), qui étaient jusqu'à présent inaccessibles par microscopie électronique ou par des microscopies à sonde locale<br>In this thesis work, we present a novel stochastic microscopy technique based on Digital Holography for the 3D mapping of optical intensity distributions. We show that this far-field, wide-field, 3D microscopy can be turned into both a superresolution and a near-field imaging technique. To do so, we use metallic nanoparticles undergoing Brownian motion as stochastic local field probes that we localize in three-dimensions in order to overcome the diffraction limit. The random motion of the particles allows for a complete exploration of the sample. Beyond simple localization, the gold markers can actually be envisaged as extremely local electromagnetic field probes, able to scatter light into the far-field. The technique we propose here is therefore a combination of the concepts of superlocalization and NSOM microscopies. The possibilities of the technique are illustrated through the 3D optical mapping of an evanescent and a propagative wave. Fast computation methods allow us to localize hundreds of particles per minute with accuracies as good as 3×3×10 nm3 for immobilized particles. In addition to optical intensity mapping, we show a particular application in electrochemistry, by coupling our high resolution images with electrochemical oxidation measurements on silver nanoparticles in solution at the vicinity of an electrode. Our results pave the way for a new subwavelength imaging technique, well adapted to optical characterization in water-based systems (such as in emerging microfluidics studies), which are mostly inaccessible to electron microscopy or local probe microscopies
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de, Leon Erich Ernesto. "Optical Design of Volume Holographic Imaging Systems for Microscopy." Diss., The University of Arizona, 2012. http://hdl.handle.net/10150/242357.

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Confocal microscopy rejects out of focus light from the object by scanning a pinhole through the object and constructing the image point by point. Volume holographic imaging (VHI) systems with bright-field illumination have been proposed as an alternative to conventional confocal type microscopes. VHI systems are an imaging modality that does not require scanning of a pinhole or a slit and thus provides video rate imaging of 3-dimensional objects. However, due to the wavelength-position degeneracy of the hologram, these systems produce less than optimal optical sectioning because the high selectivity of the volume hologram is not utilized. In this dissertation a generalized method for the design of VHI systems applied to microscopy is developed. Discussion includes the inter-relationships between the dispersive, degenerate, and depth axes of the system. Novel designs to remove the wavelength-position degeneracy and improve optical sectioning in these systems are also considered. Optimization of a fluorescence imaging system and of dual-grating confocal-rainbow designs are investigated. A ray-trace simulation that integrates the hologram diffraction efficiency and imaging results is constructed and an experimental system evaluated to demonstrate the optimization method. This results in an empirical relation between depth resolution and design tolerances. The dispersion and construction tolerances of a confocal-rainbow volume holographic imaging system are defined by the Bragg selectivity of the holograms. It is found that a broad diffraction efficiency profile of the illumination hologram with a narrow imaging hologram profile is an optimal balance between field of view, construction alignment, and depth resolution. The approach in this research is directly applicable towards imaging ovarian cells for the detection of cancer. Modeling methods, illumination design, eliminating the wavelength degeneracy of the hologram, and incorporating florescence imaging capability are emphasized in this dissertation. Results from this research may be used not only for biomedical imaging, but also for the design of volume holographic systems for both imaging and sensor applications in other fields including manufacturing (e.g. pharmaceutical), aerospace (e.g. LIDAR), and the physical sciences (e.g. climate change).
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Wu, Ning. "The use of cascaded correlation filters in holographic microscopy." Thesis, Loughborough University, 2007. https://dspace.lboro.ac.uk/2134/34156.

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Automated analysis of images collected by optical microscopes has significant potential as a straightforward and cost-effective means to screen biological samples. Depth of field restrictions, which are particularly severe in the case of high magnification and phase contrast microscopy, however, limit this approach as a means to examine more than a few nano-litres. A holographic microscope offers a solution to this problem by recording the interference between light scattered by the object field and a reference beam. In this way, all the information present in the three-dimensional scene is recorded on a single hologram without the need for mechanical scanning. A holographic microscope is thus considered as a microscope with an extended depth of field. The work presented in this thesis investigates the performance of non-linear Cascaded Correlation Filters (CCF) in two-dimensional (2D) and three-dimensional (3D) shift and rotationally invariant pattern recognition.
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Williams, Logan Andrew. "Digital Holography for Three Dimensional Tomographic and Topographic Measurements." University of Dayton / OhioLINK, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1398436841.

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Books on the topic "Holographic microscopy"

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Kim, Myung K. Digital Holographic Microscopy. Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-7793-9.

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Hans-Jochen, Foth, Marchesini R, Podbielska Halina, Society of Photo-optical Instrumentation Engineers., and European Laser Association, eds. Proceedings of optical and imaging techniques for biomonitoring II: 9-10 September 1996, Vienna, Austria. SPIE, 1996.

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Hans-Jochen, Foth, Marchesini R, Podbielska Halina, Society of Photo-optical Instrumentation Engineers., and Società italiana di laser chirurgia e medicina., eds. Proceedings of optical and imaging techniques for biomonitoring III: 6-8 September 1997, San Remo, Italy. SPIE, 1998.

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International Workshop on Electron Holography (1994 Knoxville, Tenn.). Electron holography: Proceedings of the International Workshop on Electron Holography, Holiday Inn World's Fair, Knoxville, Tennessee, USA, August 29-31, 1994. Elsevier, 1995.

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Hans-Jochen, Foth, Society of Photo-optical Instrumentation Engineers., and European Laser Association, eds. Proceedings of optical and imaging techniques for biomonitoring: 14-16 September 1995, Barcelona, Spain. SPIE, 1996.

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U.S.-Japan Seminar on Surface Characerization by Electron Diffraction, Reflection Electron Microscopy, and Holography (1993 Kona, Hawaii). Surface charaterization by LEED, RHEED, REM, STM, and holography: Proceedings of the U.S.-Japan Seminar on Surface Characterization by Electron Diffraction, Reflection Electron Microscopy, and Holography, Kona, Hawaii, 16-19 March 1993. North-Holland, 1993.

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Nowicki, Andrzej. Acoustical Imaging: Volume 31. Springer Netherlands, 2012.

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Tishko, Tatyana, and Natalya Kizilova. Holographic Microscopy of Phase Microscopic Objects: Theory and Practice. World Scientific Publishing Co Pte Ltd, 2011.

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Dmitry, Tishko, and Titar Vladimir. Holographic Microscopy of Phase Microscopic Objects: Theory and Practice. World Scientific Publishing Co Pte Ltd, 2011.

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Dmitry, Tishko, and Titar Vladimir. Holographic Microscopy of Phase Microscopic Objects: Theory and Practice. World Scientific Publishing Co Pte Ltd, 2012.

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Book chapters on the topic "Holographic microscopy"

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Colomb, Tristan, and Jonas Kühn. "Digital Holographic Microscopy." In Optical Measurement of Surface Topography. Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-12012-1_10.

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Kim, Myung K. "Digital Holographic Microscopy." In Digital Holographic Microscopy. Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-7793-9_11.

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Kim, Myung K. "Introduction." In Digital Holographic Microscopy. Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-7793-9_1.

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Kim, Myung K. "Special Techniques of Digital Holography." In Digital Holographic Microscopy. Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-7793-9_10.

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Kim, Myung K. "Low-Coherence and Tomographic Techniques." In Digital Holographic Microscopy. Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-7793-9_12.

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Kim, Myung K. "Diffraction and Fourier Optics." In Digital Holographic Microscopy. Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-7793-9_2.

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Kim, Myung K. "Principles of Holography." In Digital Holographic Microscopy. Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-7793-9_3.

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Kim, Myung K. "Basic Methods of Numerical Diffraction." In Digital Holographic Microscopy. Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-7793-9_4.

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Kim, Myung K. "Digital Holography Configurations." In Digital Holographic Microscopy. Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-7793-9_5.

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Kim, Myung K. "Theoretical Studies of Digital Holography." In Digital Holographic Microscopy. Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-7793-9_6.

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Conference papers on the topic "Holographic microscopy"

1

Falldorf, Claas, André Müller, Benat Gutierrez-Canas Pazos, Justin Bich, and Ralf B. Bergmann. "Current progress in lensless holographic microscopy." In Three-Dimensional Imaging, Visualization, and Display 2025, edited by Xin Shen, Bahram Javidi, and Arun Anand. SPIE, 2025. https://doi.org/10.1117/12.3052644.

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Bartels, Randy. "NLO Microscopy." In Nonlinear Photonics. Optica Publishing Group, 2024. https://doi.org/10.1364/np.2024.nptu2e.1.

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We demonstrate computational adaptive optical correction for second harmonic generation (SHG) and third harmonic generation (THG) holographic imaging. Results for transmission and epi SHG and transmission THG imaging will be discussed. Full-text article not available; see video presentation
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Ferraro, Pietro, Zhe Wang, Vittorio Bianco, and Pier Luca Maffettone. "Spatiotemporal digital holography microscopy fringes addresses new challenges in biological microfluidic imaging." In 3D Image Acquisition and Display: Technology, Perception and Applications. Optica Publishing Group, 2024. http://dx.doi.org/10.1364/3d.2024.jm4a.13.

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Space-Time Digital Holography (STDH) enables high-resolution biological imaging by reassembling holographic interference fringes. For imaging the flowing cells within microfluidic channel, STDH breaks through depth of focus limits and smartly adapts to microfluidic speed.
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Wuerker, R. F., and D. A. Hill. "Holographic Microscopy." In 1985 Los Angeles Technical Symposium, edited by Lloyd Huff. SPIE, 1985. http://dx.doi.org/10.1117/12.946268.

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Rastogi, Vivek, Shilpi Agarwal, Satish Dubey, Gufran Khan, and Chandra Shakher. "Microscopic urinalysis by digital holographic microscopy." In Holography, Diffractive Optics, and Applications IX, edited by Changhe Zhou, Yunlong Sheng, and Liangcai Cao. SPIE, 2019. http://dx.doi.org/10.1117/12.2537315.

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Mandracchia, B., V. Bianco, M. Paturzo, and P. Ferraro. "Holographic Microscope Slide for Single Beam Off-Axis Digital Holography Microscopy." In Frontiers in Optics. OSA, 2017. http://dx.doi.org/10.1364/fio.2017.jtu2a.2.

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Hahn, Joonku, Sehoon Lim, Kerkil Choi, Ryoichi Horisaki, Daniel L. Marks, and David J. Brady. "Compressive Holographic Microscopy." In Biomedical Optics. OSA, 2010. http://dx.doi.org/10.1364/biomed.2010.jma1.

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Chmelik, Radim. "Holographic confocal microscopy." In 12th Czech-Slovak-Polish Optical Conference on Wave and Quantum Aspects of Contemporary Optics, edited by Jan Perina, Sr., Miroslav Hrabovsky, and Jaromir Krepelka. SPIE, 2001. http://dx.doi.org/10.1117/12.417815.

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DeSantis, P., F. Gori, G. Guattari, and C. Palma. "Holographic Synthetic Microscopy." In OE LASE'87 and EO Imaging Symp (January 1987, Los Angeles), edited by Tung H. Jeong. SPIE, 1987. http://dx.doi.org/10.1117/12.939784.

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Kim, Myung. "Digital Holographic Microscopy." In Digital Holography and Three-Dimensional Imaging. OSA, 2016. http://dx.doi.org/10.1364/dh.2016.dt4g.1.

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