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1

Polak-Śliwińska, Magdalena, and Małgorzata Tańska. "Conventional and Organic Honeys as a Source of Water- and Ethanol-Soluble Molecules with Nutritional and Antioxidant Characteristics." Molecules 26, no. 12 (June 19, 2021): 3746. http://dx.doi.org/10.3390/molecules26123746.

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The benefits of natural honeybee products (e.g., honey, royal jelly, beeswax, propolis, beevenom and pollen) to the immune system are remarkable, and many of them are involved in the induction of antibody production, maturation of immune cells and stimulation of the immune system. The type of plants in the geographical area, climatic conditions and production method have a significantly influence on the nutritional quality of honey. However, this variability can influence consumer liking by the sensory attributes of the product. The aim of this work was to compare the most popular honeys from Poland in terms of nutritional value, organoleptic properties and antioxidant activity. In the study, five varieties of honey (honeydew, forest, buckwheat, linden and dandelion) from conventional and organic production methods were tested. The nutritional characteristics of honey samples included acidity, content of water, sugars, vitamin C, HMF and phenolics (total and flavonoids), while honey color, taste, aroma and consistency were investigated in the organoleptic characteristics. The antioxidant activity was determined in water- and ethanol-soluble honey extracts using DPPH and ORAC tests. The results showed that organoleptic and nutritional characteristics of popular Polish honeys differ significantly in relation to plant source and production method. The significant effect of honey variety on the content of HMF, saccharose and phenolics, as well as acidity and antioxidant capacity were noted. The impact of variety and variety × production method interaction was significant in the case of the content of vitamin C, glucose and fructose. A visible difference of buckwheat and forest honeys from other samples was observed. The highest content of total phenolics with antioxidant activity based on the SET mechanism was found in buckwheat honeys, while forest honeys were richer in flavonoids.
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2

Warui, Mary Wanjiru, Lise Hansted, Mary Gikungu, John Mburu, Geoffrey Kironchi, and Aske Skovmand Bosselmann. "Characterization of Kenyan Honeys Based on Their Physicochemical Properties, Botanical and Geographical Origin." International Journal of Food Science 2019 (January 10, 2019): 1–10. http://dx.doi.org/10.1155/2019/2932509.

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Properties and composition of honey are essential in providing information regarding their quality as well as in their differentiation based on production region characteristics, e.g., floral sources. This paper presents physicochemical properties and floral sources (botanical origin) of 21 honey samples obtained from arid and semiarid areas of Kenya, specifically, West Pokot, Baringo, and Kitui Counties. Physicochemical parameters which were analyzed to determine honey quality included moisture content, hydroxymethylfurfural (HMF), diastase activity, free acidity, and electrical conductivity. Values of these parameters were compared with those of the existing local, regional, and international standards for honey. Melissopalynological analysis (pollen analysis) was also carried out to provide information on botanical origin of the honeys. Results showed mean parameter values of moisture, 16.34%; HMF, 23.28 mg/kg; diastase activity, 10.67 Schade units; free acidity, 22.95 meq/kg; and electrical conductivity, 0.40 mS/cm. Free acidity and electrical conductivity values of honey samples obtained from West Pokot were significantly lower than the values of honeys from Baringo and Kitui. Eighteen (18) honey samples had all parameter values within the limits set in the East African, Codex Alimentarius, and the European Union directive standards for honey. Results also showed a total of 29 pollen types in the honey samples analyzed, and Acacia spp. was the predominant pollen type in 4 of the 21 honey samples. Findings of this study showed that Kenyan origin honeys can tap into the existing regional and international markets based on their quality which can be attributed to their botanical origin. Results of this study also suggested that honey producers have undertaken appropriate measures in honey harvesting, processing, handling, and storage. However, there is a need to build capacity of producers whose honey were of unacceptable quality. This would involve training on proper honey production, processing, and handling practices as well establishment of honey collection and processing centres at the local level in order to improve honey quality. This will enhance access to existing honey markets. Conservation of bee floral sources would also be needed to maintain honey quality.
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3

Sievers, Justus Thomas Obiajulu, Emily Moffat, Khadijah Yusuf, Nabaa Sarwar, Anom Bowolaksono, and Lorna Fyfe. "Comparing Manuka and Other Medical Honeys as Adjunct to Antibiotic Therapy against Facultative Anaerobes." Sains Malaysiana 51, no. 5 (May 31, 2022): 1325–37. http://dx.doi.org/10.17576/jsm-2022-5105-05.

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The development of antibiotic resistance in pathogenic bacteria has created a push for new treatments, with honeys (especially Manuka) becoming a common focus due to their strong antimicrobial action. However, alternatives to Manuka are necessary, as its production is vulnerable. Additionally, research is lacking on how honey affect facultative anaerobic bacteria grown in anaerobic conditions and how honey and antibiotics interact in these conditions. In order to understand these interactions and find novel honey candidates, we investigated the antibacterial effects of four honeys (two Manuka, one Chilean and one ‘Santa Cruz’ honeydew honey) against Staphylococcus aureus and Pseudomonas aeruginosa grown aerobically and anaerobically in broth cultures, and how the honeys affected the action of common antibiotics against these bacteria using agar diffusion assays. We found all honeys to be highly effective at 75% honey, with no significant differences between honeys, showing that other honeys were suitable alternatives to Manuka at such high concentrations. At 20%, oxygen availability and bacterial species impacted the effectiveness of honeys as Santa Cruz honey was most effective aerobically but failed anaerobically, while Manuka honeys were effective against S. aureus but not P. aeruginosa in both conditions, and Chilean honey was ineffective against all samples. The addition of honey increased bacterial sensitivity to antibiotics in some cases, varying with aerobic conditions. The antibacterial activity of the honeys, and differences in conditions whether aerobically or anaerobically, were not correlated with pH, antioxidant capacity or total phenolic count. Since in all cases honeys were either beneficial or of no effect, these results supported the use of honey as adjunct to antibiotic therapy in scenarios such as on bandages, with honeys other than Manuka also being worth consideration.
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Árváné Ványi, Georgina, Zsolt Csapó, and László Kárpáti. "Externality effects of honey production." Applied Studies in Agribusiness and Commerce 6, no. 1-2 (June 30, 2012): 63–67. http://dx.doi.org/10.19041/apstract/2012/1-2/8.

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Bee-keeping and honey production has a long history in Hungary. Honey is an important and healthy food of people and it can be consumed without any human processing. The honey production has important role, too. Some researchers say that if honey bee will extinct the humanity in the world would also extinct. It is true since plant pollination by honey bees is very important. It is confirmed by researchers’ studies that plant pollination by honey bees has significant positive external impacts on potential yields in orchards. Although the contribution of honey production to the GDP in Hungary is only a few per cent, other benefits play more important role. One of them is the positive external effect – mentioned above – and the other is the contribution to the biodiversity of the nature. This paper focuses on secondary research methods, gathering and evaluating data regarding the positive external impacts of plant pollination by honey bees as well as finding possible solution for the problem that bee-keepers have a lot of costs in connection with carrying honey bees to orchards, while farmers “only” benefit from the positive externality of plant pollination of their fields. To evaluate its economic effects a numerical HEEM-model was developed and applied for the Hungarian situation.
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Wolditsadik, Mekonnen, Taye Beyene, and Desta Abi. "Establishing Honey Bee Floral Calendar in West Arsi and East Shewa Zones of Oromia, Ethiopia." Cross Current International Journal of Agriculture and Veterinary Sciences 4, no. 3 (June 29, 2022): 25–33. http://dx.doi.org/10.36344/ccijavs.2022.v04i03.002.

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Adequate knowledge about honey bee flora is important for beekeeping this study was under taken to identify and document honeybee plants in West Arsi and East Shoa Zone using melissopalynological analysis of honey samples, pollen collection, plant inventory, structured questionnaires and field observation. Eighteen honey samples were collected from different parts of the zones. Out of 18 samples, 14 were identified as monofloral honeys and 4 as multifloral honeys. This indicated that there is high diversity of honeybee plant species in 4 honey samples that give flowers in the same season contributing for production of multifloral honey and 14 samples were dominated by few major honeybee plants due to their abundance in addition to their quality for honey production. Fifty eight (58) plants species were identified as honey source plants based on melissopalynological analysis of honey. Of 58 species, Guizotia scarba (89.7%). Eucalyptus (69.2%) Eucalyptus camadulensis (66.2%) Acacia tortolis (60.9%) Schefflera Abyssinia (70.2%) and Croton macronstachys (57.2%) provided mono-floral honey. The flowering calendar of the zone indicated two major honey flow periods from April to June and September to November. The scarcity of honeybee forages were observed in July to mid of August and January to February and mid of March. In many districts of the zone, herbaceous honeybee forage species were the dominant honey source plants in September to November. While, in March to May majority of honey source plants were trees and shrubs due to the phonological patterns of plants. To apply seasonal honeybee colony management, beekeepers should manage honeybee colonies following phonological pattern of honeybee plants. Beekeeping based agro forestry practices on cultivated rain fed land, and selection of plant species that can resist drought and bear (set) flower for a long season should be introduced in all land use types.
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Majtan, Juraj, Martin Sojka, Helena Palenikova, Marcela Bucekova, and Viktor Majtan. "Vitamin C Enhances the Antibacterial Activity of Honey against Planktonic and Biofilm-Embedded Bacteria." Molecules 25, no. 4 (February 23, 2020): 992. http://dx.doi.org/10.3390/molecules25040992.

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Multifactorial antibacterial action is an important feature of honey; however, its bactericidal efficacy against biofilm-embedded bacteria is limited. The aim of this study was to investigate the impact of vitamin C (Vit C) on the antibacterial activity of natural honeys against planktonic as well as biofilm-embedded bacterial pathogens. The antibacterial activity of four honey samples supplemented with Vit C was expressed as the minimum inhibitory concentration (MIC). At sub-MICs, Vit C significantly increased the antibacterial activity of the tested honeys against Pseudomonas aeruginosa in planktonic cultures. However, after supplementation, honeydew honey, the most active honey, was ineffective against Staphylococcus aureus. On the other hand, when 100% honeydew honey was supplemented with Vit C (100 mg/g of honey) in a multispecies wound biofilm model, complete eradication of almost all bacterial isolates, including S. aureus, was observed. Furthermore, a mixture of honey and Vit C was partially effective against Enterococcus faecalis, whereas honey alone exhibited no antibacterial activity against this bacterium. Vit C counteracted hydrogen peroxide in honey solution and, thus, eliminated the major antibacterial compound present in honey. It is likely that a combination of honey with Vit C may trigger the intracellular production of reactive oxygen species in bacterial cells, but the exact cellular mechanisms warrant further investigations.
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Ivana, Valachová, Bučeková Marcela, and Majtán Juraj. "Quantification of bee-derived peptide defensin-1 in honey by competitive enzyme-linked immunosorbent assay, a new approach in honey quality control." Czech Journal of Food Sciences 34, No. 3 (June 17, 2016): 233–43. http://dx.doi.org/10.17221/422/2015-cjfs.

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We established and evaluated a polyclonal antibody based competitive enzyme-linked immunosorbent assay for the quantification of defensin-1 in honey. The assay showed an inhibitory concentration (IC<sub>50</sub>) value of 111.5 ± 15.41 ng/ml with a detection limit of 7.8125 ng/ml. The regaining of defensin-1 in spiked ‘artificial honey’ was between 87.05 and 112.96% with relative standard deviation less than 9.2%. Sensitivity and specificity of the test were experimentally validated on a sample of 20 different honeys. The antibacterial activity of these honey samples showed a significant concentration-dependent correlation with the production of defensin-1 (n = 20; r = −0.6598; P = 0.0016). The assay provides a specific and sensitive method for the screening of defensin-1 in honey. The method to detect honeybee-derived proteins in honey is a promising approach to verifying the authenticity of honey. The defensin-1 ELISA could also be used for the rapid screening of honeys suitable for medicinal purposes.
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Franz, Gabriela Maria, Carla Galbiati, Elias Araujo, and Ligia Bicudo de Almeida-Muradian. "Antibacterial activity and physicochemical characteristics of honey from Mato Grosso’s Amazon, Cerrado and Pantanal, Brazil." Revista Ibero-Americana de Ciências Ambientais 9, no. 1 (May 22, 2018): 26–42. http://dx.doi.org/10.6008/spc2179-6858.2018.001.0003.

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The objective of this is report was to evaluate the physicochemical characteristics and the antibacterial activity of honey produced for human consumption in some counties in Amazon, Cerrado and Pantanal of Mato Grosso, Brazil. Honey samples were collected in 6 municipalities, totaling 18 samples, 3 samples per municipality. The physicochemical characteristics evaluated were moisture, reducing sugars, apparent sucrose, glucose and fructose by HPLC system, ashes, insoluble solids, free acidity, HMF, diastase activity, Lund, Fiehe and Lugol reactions, electrical conductivity and color. The content of total phenolic and flavonoids were quantified using standard curve of gallic acid and quercetin. The antibacterial activity of honey was evaluated by disk diffusion to five bacteria. Fifteen out of eighteen samples were reproved in some of the physicochemical characteristics. Honeys’ average content of total phenolic was 35.68 mg GAE/100 g of honey. The content of total phenolic and flavonoids showed positive correlation with the color of the honeys. The studied honeys did not present antibacterial activity, but the phenolic and flavonoid contents originate potential antioxidant and prebiotic for the darkest honeys. Mato Grosso has potential to the production of the floral honey, in need of adequacy on the quality of the honey to the physicochemical characteristic’s patterns.
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9

ADAMCHUK, Leonora. "MELISSOPALINOLOGICAL STUDY OF SOUTHERN BESSARABIA HONEY." Herald of Khmelnytskyi National University 305, no. 1 (February 23, 2022): 20–24. http://dx.doi.org/10.31891/2307-5732-2022-305-1-20-24.

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Different natural and climatic zones, directions of agricultural production, ethnic and cultural features of agriculture, and reliefs of the regions of Ukraine are the result of a wide variety of vegetation. Ukraine occupies less than 6% of Europe’s area but owns about 35% of all its biodiversity. This leads to a considerable list of plants that are sources of nectar for the production of honey of different varieties. Ukraine produces classic monofloral varieties of honey, such as white acacia, rapeseed, linden, buckwheat, sunflower, as well as less common monofloral, such as mustard, clover, raspberry, bird’s-foot, phacelia, fireweed, goldenrod, sophora, honeydew. Today, regional polyfloral varieties of honey are also produced, such as Carpathian, steppe, Crimean, and other locally produced honey from reserves (for example, from Gomilshansky forests, Mezynsky reserve, Askanian meadows). A common way to identify the botanical and geographical origin of honey and identify its species is to study the pollen composition. This work was aimed to determine the pollen profile of spring honeys of Southern Bessarabia using the method of melissopalynology. Bessarabia is an ethnographic region with its own culture of agricultural production and natural and climatic conditions. The material of the study was samples of honey obtained in the apiaries of the Center for Ethnographic, Green, Rural Tourism, and Family Recreation Frumushika-Nova (Odessa region) in 2021. The pollen profile of honey was investigated using the author’s improved method of melissopalynological analysis. Identification of pollen grains was performed using the online database of pollen grains PalDat using a model nutrient medium. The pollen profile of spring honeys of Southern Bessarabia is represented by 30 morphotypes of pollen grains of different plant species, contains honeydew of natural origin and natural impurities (plant particles, wax, bee bodies). In the pollen profile of honey, no dominant (45% or more) pollen grains were detected, secondary – 6, insignificant – 21, inclusions – 3 morphotypes. This allows us to assert the polyflorality of the studied honey with the exception of white acacia, the dominant pollen of which may be 20% or more. Pollen grains of endemic species of steppe plants of Ukraine were found in the pollen profile of spring honeys of Southern Bessarabia (tamarix ramosissima, lathyrus pannonicus, rosa tesquicola, bifora radians, ailanthus altissima, ultus pumila, prunus tenella, viola occulta). The obtained results can be further used to identify the geographical origin of honey from this region. At the next stages of the research, it will be promising to determine the pollen profiles of summer honeys of Southern Bessarabia and other ethnographic regions of Ukraine.
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Bareke, Tura, and Admassu Addi. "Pollen Analysis of Honey from Borana Zone of Southern Ethiopia." Journal of Apicultural Science 63, no. 2 (December 1, 2019): 233–42. http://dx.doi.org/10.2478/jas-2019-0022.

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AbstractNineteen samples of honey were collected from different localities of the Borana Zone and examined to identify the botanical origin of honey through honey pollen analysis. From nineteen honey samples, sixteen were identified as monofloral honeys. Twentyeight plant species were identified as honey source plants and the identified plant species belonged to fourteen plant families. Out of twenty-eight bee plant species, 17.9% of them were found in the Fabaceae family followed by Asteraceae and Lamiaceae, each of them accounting for 14.3% of all honey plants species found in the samples. The Shannon-Wiener diversity index (H) showed that high diversity of plant species was found in eleven honey samples with a range of 1.07 (Bule Hora site 1) to 1.81 (Yabello site 2) on the basis of honey pollen analysis. Eight honey samples had lower diversity index values, ranging from 0 (Arero site 2 and Bule Hora site 3) to 0.84 (Gelana site1), which suggests the honey was obtained from a few dominant plant species. Accordingly, Guizotia scabra, Haplocoelum foliolosum, Plectranthus assurgens, Terminalia brownii, Sesamum indicum, Satureja paradoxa, Croton macrostachyus and Acacia brevispica were the major monofloral honeys produced from the area. This indicates that there is a huge potential for the production of monofloral honey. Since monofloral honey has a good market value and is preferred by consumers, the involvement of investors is recommended.
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Kostenko, Olga. "Beekeeping and honey production in Russia." BIO Web of Conferences 48 (2022): 02007. http://dx.doi.org/10.1051/bioconf/20224802007.

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Honey occupies a special place in the diet due to its functional and medicinal properties. Honey consumption is significantly below the level recommended both in Russia and in the world. It is possible to significantly increase honey consumption both in the internal and foreign markets. The research aims to analyze and identify problems, obstacles and driving forces in the development of beekeeping in the case of Russia. Analysis showed that 18 Russian regions are focused on the export of honey to interregional and foreign markets. The beekeeping sector faces significant challenges in honey selling. The adulterated honey share is estimated at 30%. More than half of the honey on the Russian market is sold through direct short channels. Promoting products to metropolitan and foreign markets requires new approaches to building supply chains: data on apiaries and honey quality; optimizing supply chains and income distribution by chain links. The industry drivers can be a cluster approach; digital technologies to solve specific industry issues; new approaches in support of the industry by state.
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García-Seval, Víctor, Javier Saurina, Sònia Sentellas, and Oscar Núñez. "Off-Line SPE LC-LRMS Polyphenolic Fingerprinting and Chemometrics to Classify and Authenticate Spanish Honey." Molecules 27, no. 22 (November 13, 2022): 7812. http://dx.doi.org/10.3390/molecules27227812.

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The feasibility of non-targeted off-line SPE LC-LRMS polyphenolic fingerprints to address the classification and authentication of Spanish honey samples based on both botanical origin (blossom and honeydew honeys) and geographical production region was evaluated. With this aim, 136 honey samples belonging to different botanical varieties (multifloral and monofloral) obtained from different Spanish geographical regions with specific climatic conditions were analyzed. Polyphenolic compounds were extracted by off-line solid-phase extraction (SPE) using HLB (3 mL, 60 mg) cartridges. The obtained extracts were then analyzed by C18 reversed-phase LC coupled to low-resolution mass spectrometry in a hybrid quadrupole-linear ion trap mass analyzer and using electrospray in negative ionization mode. Principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) were employed to assess the pattern recognition capabilities of the obtained fingerprints to address honey classification and authentication. In general, a good sample discrimination was accomplished by PLS-DA, being able to differentiate both blossom-honey and honeydew-honey samples according to botanical varieties. Multiclass predictions by cross-validation for the set of blossom-honey samples showed sensitivity, specificity, and classification ratios higher than 60%, 85%, and 87%, respectively. Better results were obtained for the set of honeydew-honey samples, exhibiting 100% sensitivity, specificity, and classification ratio values. The proposed fingerprints also demonstrated that they were good honey chemical descriptors to deal with climatic and geographical issues. Characteristic polyphenols of each botanical variety were tentatively identified by LC-MS/MS in multiple-reaction monitoring mode to propose possible honey markers for future experiments (i.e., naringin for orange/lemon blossom honeys, syringic acid in thyme honeys, or galangin in rosemary honeys).
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Bobiş, Otilia, Victoriţa Bonta, Mihaiela Cornea-Cipcigan, Gulzar Ahmad Nayik, and Daniel Severus Dezmirean. "Bioactive Molecules for Discriminating Robinia and Helianthus Honey: High-Performance Liquid Chromatography–Electron Spray Ionization–Mass Spectrometry Polyphenolic Profile and Physicochemical Determinations." Molecules 26, no. 15 (July 22, 2021): 4433. http://dx.doi.org/10.3390/molecules26154433.

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Bioactive molecules from the class of polyphenols are secondary metabolites from plants. They are present in honey from nectar and pollen of flowers from where honeybees collect the “raw material” to produce honey. Robinia pseudoacacia and Helianthus annuus are important sources of nectar for production of two monofloral honeys with specific characteristics and important biological activity. A high-performance liquid chromatography–electro spray ionization–mass spectrometry (HPLC–ESI–MS) separation method was used to determine polyphenolic profile from the two types of Romanian unifloral honeys. Robinia and Helianthus honey showed a common flavonoid profile, where pinobanksin (1.61 and 1.94 mg/kg), pinocembrin (0.97 and 1.78 mg/kg) and chrysin (0.96 and 1.08 mg/kg) were identified in both honey types; a characteristic flavonoid profile in which acacetin (1.20 mg/kg), specific only for Robinia honey, was shown; and quercetin (1.85 mg/kg), luteolin (21.03 mg/kg), kaempferol (0.96 mg/kg) and galangin (1.89 mg/kg), specific for Helianthus honey, were shown. In addition, different phenolic acids were found in Robinia and Helianthus honey, while abscisic acid was found only in Robinia honey. Abscisic acid was correlated with geographical location; the samples collected from the south part of Romania had higher amounts, due to climatic conditions. Acacetin was proposed as a biochemical marker for Romanian Robinia honey and quercetin for Helianthus honey.
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White, Jonathan W., Kenneth Winters, Martin Peter, and Andreas Rossmann. "Stable Carbon Isotope Ratio Analysis of Honey: Validation of Internal Standard Procedure for Worldwide Application." Journal of AOAC INTERNATIONAL 81, no. 3 (May 1, 1998): 610–19. http://dx.doi.org/10.1093/jaoac/81.3.610.

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abstract Stable carbon isotope ratio analysis (SCIRA) of honey for undeclared presence of cane or corn sugars has been available for 20 years. Its use with domestic and imported honeys is reviewed. Six years of data from the internal standard isotope ratio analysis (ISCIRA) method support its worldwide validity for honey analysis. The ISCIRA database of pure honeys has been increased from 64 U.S. samples to 224 by addition of data from Germany, United Kingdom, Mexico, Italy, and Spain. ISCIRA analyses of 131 commercial honeys from the United States, Mexico, and Spain found that 17 are adulterated. Analyses of 303 Chinese honeys proves that they should have carbon isotope values similar to honeys from other areas, contrary to claims that the observed differences are intrinsic because of the variability of environmental conditions and of plants used in honey production in China. Addition of corn or cane (C4) sugars to honeys in amounts that do not produce a δ13C value greater than -23.5%o for the mixture cannot be detected by the original 1978 SCIRA procedure. Such adulteration however is detected by ISCIRA procedure from the δ13C value of the protein contained in the honey, which shows the isotopic composition of the honey before addition of C4 sugars. Fortythree percent of 98 honeys received in the United States in 1994-1997 with δ13C &lt; -23.5%o were suspected and found to be adulterated.
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S, Fikru. "Review of Honey Bee and Honey Production in Ethiopia." Journal of Animal Science Advances 5, no. 10 (2015): 1413. http://dx.doi.org/10.5455/jasa.20151019083635.

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Zucchetta, Claire, Wally Tangohau, Aaron McCallion, Derrylea J. Hardy, and Andrea Clavijo McCormick. "Exploring the Chemical Properties and Biological Activity of Four New Zealand Monofloral Honeys to Support the Māori Vision and Aspirations." Molecules 27, no. 10 (May 20, 2022): 3282. http://dx.doi.org/10.3390/molecules27103282.

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Honey production and export are significant contributors to the Aotearoa New Zealand economy, generating over 400 million dollars in revenue. Its main export is mānuka (Leptospermum scoparium) honey, which has a high commercial value due to its medicinal properties that are linked to its unique chemical composition. The compound methylglyoxal (MGO) has been identified as the main floral marker and is used as a quality indicator, often labelled as unique mānuka factor (UMF). However, the high demand for mānuka honey creates pressure on beekeepers and may have negative ecological consequences by favouring extensive mānuka monocultures to the detriment of other native species. There are other honeys native to New Zealand, such as kāmahi (Weinmannia racemosa), kānuka (Kunzea ericoides), rātā (Metrosideros robusta) and rewarewa (Knightia excelsa), that also have medicinal properties; however, they are less well known in the local and global market. Indigenous Māori communities envision the production and commercialization (locally and internationally) of these honeys as an opportunity to generate income and secure a sustainable future in alignment with their worldview (Te Ao Māori) and values (tikanga Māori). Diversifying the market could lead to a more sustainable income for beekeepers and reduce pressure on Māori and the conservation land, while supporting indigenous communities to realize their vision and aspirations. This manuscript provides an extensive review of the scientific literature, technical literature and traditional knowledge databases describing the plants of interest and their traditional medicinal uses (rongoā) and the chemical properties of each honey, potential floral markers and their biological activity. For each honey type, we also identify knowledge gaps and potential research avenues. This information will assist Māori beekeepers, researchers, consumers and other stakeholders in making informed decisions regarding future research on and the production, marketing and consumption of these native monofloral honeys.
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Girelli, Chiara Roberta, Roberta Schiavone, Sebastiano Vilella, and Francesco Paolo Fanizzi. "Salento Honey (Apulia, South-East Italy): A Preliminary Characterization by 1H-NMR Metabolomic Fingerprinting." Sustainability 12, no. 12 (June 18, 2020): 5009. http://dx.doi.org/10.3390/su12125009.

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Honey is a natural sweet substance produced by honeybees from the nectar of flowers, plant secretions or plant-sucking insect excretions. Sugars and water constitute the major components, other minor components characterize the organoleptic and nutritional properties. To date, Salento (Apulia region, Italy) honey production is considerably threatened due to the suggested use of neonicotinoids in order to control the insect-vectored bacterium Xylella fastidiosa (subsp. pauca). Metabolomics based on Nuclear Magnetic Resonance (NMR) spectroscopy was used to describe, for the first time, the composition of honey samples from different Salento producers. Exploratory Principal Component Analysis (PCA) showed, among the observed clustering, a separation between light and dark honeys and a discrimination according to producers, both further analyzed by supervised multivariate analysis. According to the obtained data, although limited to small-scale emerging production, Salento honey shows at the molecular level, a range of specific characteristic features analogous to those exhibited by similar products originating elsewhere and appreciated by consumers. The impact on this production should therefore be carefully considered when suggesting extensive use of pesticides in the area.
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Brudzynski, Katrina. "Effect of hydrogen peroxide on antibacterial activities of Canadian honeys." Canadian Journal of Microbiology 52, no. 12 (December 1, 2006): 1228–37. http://dx.doi.org/10.1139/w06-086.

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Honey is recognized as an efficacious topical antimicrobial agent in the treatment of burns and wounds. The antimicrobial activity in some honeys depends on the endogenous hydrogen peroxide content. This study was aimed to determine whether honey's hydrogen peroxide level could serve as a honey-specific, activity-associated biomarker that would allow predicting and assessing the therapeutic effects of honey. Using a broth microdilution assay, I analyzed antibacterial activities of 42 Canadian honeys against two bacterial strains: Escherichia coli (ATCC 14948) and Bacillus subtilis (ATCC 6633). The MIC90 and MIC50 were established from the dose-response relationship between antibacterial activities and honey concentrations. The impact of H2O2 on antibacterial activity was determined (i) by measuring the levels of H2O2 before and after its removal by catalase and (ii) by correlating the results with levels of antibacterial activities. Canadian honeys demonstrated moderate to high antibacterial activity against both bacterial species. Both MIC90 and MIC50 revealed that the honeys exhibited a selective growth inhibitory activity against E. coli, and this activity was strongly influenced by endogenous H2O2 concentrations. Bacillus subtilis activity was marginally significantly correlated with H2O2 content. The removal of H2O2 by catalase reduced the honeys' antibacterial activity, but the enzyme was unable to completely decompose endogenous H2O2. The 25%-30% H2O2 "leftover" was significantly correlated with the honeys' residual antibacterial activity against E. coli. These data indicate that all Canadian honeys exhibited antibacterial activity, with higher selectivity against E. coli than B. subtilis, and that these antibacterial activities were correlated with hydrogen peroxide production in honeys. Hydrogen peroxide levels in honey, therefore, is a strong predictor of the honey's antibacterial activity.Key words: honey, antibacterial activity, hydrogen peroxide, catalase, Escherichia coli, Bacillus subtilis.
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García-Seval, Víctor, Javier Saurina, Sònia Sentellas, and Oscar Núñez. "Characterization and Classification of Spanish Honey by Non-Targeted LC–HRMS (Orbitrap) Fingerprinting and Multivariate Chemometric Methods." Molecules 27, no. 23 (November 30, 2022): 8357. http://dx.doi.org/10.3390/molecules27238357.

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A non-targeted LC–HRMS fingerprinting methodology based on a C18 reversed-phase mode under universal gradient elution using an Orbitrap mass analyzer was developed to characterize and classify Spanish honey samples. A simple sample treatment consisting of honey dissolution with water and a 1:1 dilution with methanol was proposed. A total of 136 honey samples belonging to different blossom and honeydew honeys from different botanical varieties produced in different Spanish geographical regions were analyzed. The obtained LC–HRMS fingerprints were employed as sample chemical descriptors for honey pattern recognition by principal component analysis (PCA) and partial least squares–discriminant analysis (PLS–DA). The results demonstrated a superior honey classification and discrimination capability with respect to previous non-targeted HPLC–UV fingerprinting approaches, with them being able to discriminate and authenticate the honey samples according to their botanical origins. Overall, noteworthy cross-validation multiclass predictions were accomplished with sensitivity and specificity values higher than 96.2%, except for orange/lemon blossom (BL) and rosemary (RO) blossom-honeys. The proposed methodology was also able to classify and authenticate the climatic geographical production region of the analyzed honey samples, with cross-validation sensitivity and specificity values higher than 87.1% and classification errors below 10.5%.
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Mărgăoan, Rodica, Erkan Topal, Ralitsa Balkanska, Banu Yücel, Titanilla Oravecz, Mihaiela Cornea-Cipcigan, and Dan Cristian Vodnar. "Monofloral Honeys as a Potential Source of Natural Antioxidants, Minerals and Medicine." Antioxidants 10, no. 7 (June 25, 2021): 1023. http://dx.doi.org/10.3390/antiox10071023.

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Background: vegetative diversity is based on different climate and geographical origins. In terms of beekeeping, herbal diversity is strongly correlated to the production of a wide variety of honey. Therefore, based on the existing plant diversity in each country, multiple honey varieties are produced with different health characteristics. While beekeeping potential and consumption preferences are reflected in products’ variety, this leads to an increase in the region’s economy and extensive export. In the last years, monofloral honey has gained interest from consumers and especially in the medicinal field due to the presence of phytochemicals which are directly linked to health benefits, wound healing, antioxidant, anticancer and anti-inflammatory activities. Scope and approach: this review aims to highlight the physicochemical properties, mineral profiles and antioxidant activities of selected monofloral honeys based on their botanical and geographical origin. Moreover, this review focuses on the intercorrelation between monofloral honey’s antioxidant compounds and in vitro and in vivo activities, focusing on the apoptosis and cell proliferation inhibition in various cell lines, with a final usage of honey as a potential therapeutic product in the fight towards reducing tumor growth. Key findings and conclusions: multiple studies have demonstrated that monofloral honeys have different physicochemical structures and bioactive compounds. Useful chemical markers to distinguish between monofloral honeys were evidenced, such as: 2-methoxybenzoic acid and trimethoxybenzoic acid are distinctive to Manuka honey while 4-methoxyphenylacetic acid is characteristic to Kanuka honey. Furthermore, resveratrol, epigallocatechin and pinostrobin are markers distinct to Sage honey, whereas carvacrol and thymol are found in Ziziphus honey. Due to their polyphenolic profile, monofloral honeys have significant antioxidant activity, as well as antidiabetic, antimicrobial and anticancer activities. It was demonstrated that Pine honey decreased the MDA and TBARS levels in liver, kidney, heart and brain tissues, whereas Malicia honey reduced the low-density lipoprotein level. Consumption of Clover, Acacia and Gelam honeys reduced the weight and adiposity, as well as trygliceride levels. Furthermore, the antiproliferative effect of chrysin, a natural flavone in Acacia honey, was demonstrated in human (A375) and murine (B16-F1) melanoma cell lines, whereas caffeic acid, a phenolic compound found in Kelulut honey, proves to be significant candidate in the chemoprevention of colon cancer. Based on these features, the use of hiney in the medicinal field (apitherapy), and the widespread usage of natural product consumption, is gaining interest by each year.
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Huang, Zhenxiong, Lang Liu, Guojian Li, Hong Li, Dapeng Ye, and Xiaoli Li. "Nondestructive Determination of Diastase Activity of Honey Based on Visible and Near-Infrared Spectroscopy." Molecules 24, no. 7 (March 29, 2019): 1244. http://dx.doi.org/10.3390/molecules24071244.

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The activities of enzymes are the basis of evaluating the quality of honey. Beekeepers usually use concentrators to process natural honey into concentrated honey by concentrating it under high temperatures. Active enzymes are very sensitive to high temperatures and will lose their activity when they exceed a certain temperature. The objective of this work is to study the kinetic mechanism of the temperature effect on diastase activity and to develop a nondestructive approach for quick determination of the diastase activity of honey through a heating process based on visible and near-infrared (Vis/NIR) spectroscopy. A total of 110 samples, including three species of botanical origin, were used for this study. To explore the kinetic mechanism of diastase activity under high temperatures, the honey of three kinds of botanical origins were processed with thermal treatment to obtain a variety of diastase activity. Diastase activity represented with diastase number (DN) was measured according to the national standard method. The results showed that the diastase activity decreased with the increase of temperature and heating time, and the sensitivity of acacia and longan to temperature was higher than linen. The optimum temperature for production and processing is 60 °C. Unsupervised clustering analysis was adopted to detect spectral characteristics of these honeys, indicating that different botanical origins of honeys can be distinguished in principal component spaces. Partial least squares (PLS) and least squares-support vector machine (LS-SVM) algorithms were applied to develop quantitative relationships between Vis/NIR spectroscopy and diastase activity. The best result was obtained through Gaussian filter smoothing-standard normal variate (GF-SNV) pretreatment and the LS-SVM model, known as GF-SNV-LS-SVM, with a determination coefficient (R2) of prediction of 0.8872, and root mean square error (RMSE) of prediction of 0.2129. The overall results of this paper showed that the diastase activity of honey can be determined quickly and non-destructively with Vis/NIR spectral methods, which can be used to detect DN in the process of honey production and processing, and to maximize the nutrient content of honey.
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Corey, Kris, Anwar A. Hamama, Haiwen Li, Rafat A. Siddiqui, Chyer Kim, and Harbans L. Bhardwaj. "Composition of Buckwheat Honey." Journal of Agricultural Science 14, no. 9 (August 15, 2022): 59. http://dx.doi.org/10.5539/jas.v14n9p59.

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Buckwheat has been grown in Virginia since late 1700s; however, today the crop is almost non-existent in Virginia. Since buckwheat flowers profusely in a few weeks after planting, it has potential to support honeybees but there is a lack of information about quality of buckwheat honey produced in Virginia. Our objective was to characterize composition of honey produced by honeybees foraging on buckwheat (Buckwheat honey), compared to that produced by honeybees foraging on wild plants (Wild plant honey). Buckwheat honey differed in composition, antioxidant concentrations, and microbial activities from wild plant honey. Concentrations of fructose, glucose, and melezitos in buckwheat honey were quantitatively lower than that in wild plant honey whereas concentrations of sucrose and maltose exhibited an opposite trend&mdash;concentration of maltose being statistical significant. Fructose was the dominant sugar (42 and 52 percent in buckwheat honey and wild plant honey, respectively). Buckwheat honey had significant higher concentrations of K and Cu in comparison to wild plant honey (0.17 and 0.04 percent, and 5.0 and 3.33 ppm, respectively). Concentrations of Trolox and TPC were significantly higher in buckwheat honey than wild plant honey (1.01 and 0.32, and 0.39 and 0.17, respectively). Both types of honeys exhibited anti-microbial activity against gram-positive and gram-negative bacteria. The buckwheat honey was darker in color than the honey from wild plants. We concluded that production of buckwheat as a grain or cover crop can also support honeybees and buckwheat honey might be superior to wild plant honey.
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Borges, Cristine Vanz, Aline Nunes, Vladimir Eliodoro Costa, Ricardo de Oliveira Orsi, Leticia Silva Pereira Basilio, Gean Charles Monteiro, Marcelo Maraschin, and Giuseppina Pace Pereira Lima. "Tryptophan and Biogenic Amines in the Differentiation and Quality of Honey." International Journal of Tryptophan Research 15 (January 2022): 117864692211020. http://dx.doi.org/10.1177/11786469221102098.

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Honey is a natural product with beneficial properties to health and has different characteristics depending on the region of production and collection, flowering, and climate. The presence of precursor amino acids of- and biogenic amines can be important in metabolomic studies of differentiation and quality of honey. We analyzed 65 honeys from 11 distinct regions of the State of Santa Catarina (Brazil) as to the profile of amino acids and biogenic amines by HPLC. The highest L-tryptophan (Trp), 5-hydroxytryptophan (5-OH-Trp), and tryptamine (Tryp) levels were detected in Cfb climate and harvested in 2019. Although we have found high content of serotonin, dopamine, and L-dopa in Cfb climate, the highest values occurred in honey produced during the summer 2018 and at altitudes above 900 m. Results indicate that the amino acids and biogenic amine levels in honeys are good indicators of origin. These data warrant further investigation on the honey as source of amino acids precursor of serotonin, melatonin, and dopamine, what can guide the choice of food as source of neurotransmitters.
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Guttentag, Annabel, Krishothman Krishnakumar, Nural Cokcetin, Steven Hainsworth, Elizabeth Harry, and Dee Carter. "Inhibition of Dermatophyte Fungi by Australian Jarrah Honey." Pathogens 10, no. 2 (February 11, 2021): 194. http://dx.doi.org/10.3390/pathogens10020194.

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Superficial dermatophyte infections, commonly known as tineas, are the most prevalent fungal ailment and are increasing in incidence, leading to an interest in alternative treatments. Many floral honeys possess antimicrobial activity due to high sugar, low pH, and the production of hydrogen peroxide (H2O2) from the activity of the bee-derived enzyme glucose oxidase. Australian jarrah (Eucalyptus marginata) honey produces particularly high levels of H2O2 and has been found to be potently antifungal. This study characterized the activity of jarrah honey on fungal dermatophyte species. Jarrah honey inhibited dermatophytes with minimum inhibitory concentrations (MICs) of 1.5–3.5% (w/v), which increased to ≥25% (w/v) when catalase was added. Microscopic analysis found jarrah honey inhibited the germination of Trichophyton rubrum conidia and scanning electron microscopy of mature T. rubrum hyphae after honey treatment revealed bulging and collapsed regions. When treated hyphae were stained using REDOX fluorophores these did not detect any internal oxidative stress, suggesting jarrah honey acts largely on the hyphal surface. Although H2O2 appears critical for the antifungal activity of jarrah honey and its action on fungal cells, these effects persisted when H2O2 was eliminated and could not be replicated using synthetic honey spiked with H2O2, indicating jarrah honey contains agents that augment antifungal activity.
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Labsvards, Kriss Davids, Vita Rudovica, Rihards Kluga, Janis Rusko, Lauma Busa, Maris Bertins, Ineta Eglite, Jevgenija Naumenko, Marina Salajeva, and Arturs Viksna. "Determination of Floral Origin Markers of Latvian Honey by Using IRMS, UHPLC-HRMS, and 1H-NMR." Foods 11, no. 1 (December 24, 2021): 42. http://dx.doi.org/10.3390/foods11010042.

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The economic significance of honey production is crucial; therefore, modern and efficient methods of authentication are needed. During the last decade, various data processing methods and a combination of several instrumental methods have been increasingly used in food analysis. In this study, the chemical composition of monofloral buckwheat (Fagopyrum esculentum), clover (Trifolium repens), heather (Calluna vulgaris), linden (Tilia cordata), rapeseed (Brassica napus), willow (Salix cinerea), and polyfloral honey samples of Latvian origin were investigated using several instrumental analysis methods. The data from light stable isotope ratio mass spectrometry (IRMS), ultra-high performance liquid chromatography coupled with high-resolution mass spectrometry (UHPLC-HRMS), and nuclear magnetic resonance (NMR) analysis methods were used in combination with multivariate analysis to characterize honey samples originating from Latvia. Results were processed using the principal component analysis (PCA) to study the potential possibilities of evaluating the differences between honey of different floral origins. The results indicate the possibility of strong differentiation of heather and buckwheat honeys, and minor differentiation of linden honey from polyfloral honey types. The main indicators include depleted δ15N values for heather honey protein, elevated concentration levels of rutin for buckwheat honey, and qualitative presence of specific biomarkers within NMR for linden honey.
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Nag, Nitin Kumar. "An Economics Analysis of Honey Production in Baghpat District of Western Uttar Pradesh." Indian Journal of Pure & Applied Biosciences 10, no. 1 (February 28, 2022): 47–53. http://dx.doi.org/10.18782/2582-2845.8876.

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This study of Beekeeping, considered to be a 'no investment-profit-giving' venture, is an age-old tradition in India. The diversity of Bee- flora and varied agro-climate conditions in Uttar Pradesh offers enormous potential for the development and growth of apiculture. The district has six development blocks out of which two bocks, namely Baraut and Baghpat blocks were selected randomly for the study. Keeping low number of Honey bee producers in the study area, all 10 Honey bee producers. On the basis of Honey box unit, these bee producers were categories in two categories, i.e. 1-100 box and 101-200 box. The primary data were collected by survey method with respondents through scheduled personal interviews. The income from honey / box / annum came to Rs. 8077.36 in 1-100 box category while Rs. 8514.36 in 101-200 box category and the average income / box / annum came to Rs. 8295.83. The table also shows the category wise input-output ratio. The average of Input-Output Ratio came out to 1:3.65, while category wise, it came to 1:3.45 for 1-100 box category and 1:3.85 for 101-200 box category. The Operational cost of honey production/kg came to Rs. 57.87 for 1-100 box category while Rs. 50.91 for 101- 200 box category. The average operational cost came to Rs. 54.39. The operational cost of 1-100 box category and 101-200 box category came to Rs. 162.13 and Rs. 169.09 respectively. The average return over operational cost came to Rs. 165.61. This study is that Honey Production is a good combination for mixed farming because it requires less area of land, small capital, and high return. So those farmers who have less area of land should adopt honey production as a subsidiary occupation.
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Nolan, Victoria C., James Harrison, and Jonathan A. G. Cox. "Dissecting the Antimicrobial Composition of Honey." Antibiotics 8, no. 4 (December 5, 2019): 251. http://dx.doi.org/10.3390/antibiotics8040251.

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Honey is a complex sweet food stuff with well-established antimicrobial and antioxidant properties. It has been used for millennia in a variety of applications, but the most noteworthy include the treatment of surface wounds, burns and inflammation. A variety of substances in honey have been suggested as the key component to its antimicrobial potential; polyphenolic compounds, hydrogen peroxide, methylglyoxal and bee-defensin 1. These components vary greatly across honey samples due to botanical origin, geographical location and secretions from the bee. The use of medical grade honey in the treatment of surface wounds and burns has been seen to improve the healing process, reduce healing time, reduce scarring and prevent microbial contamination. Therefore, if medical grade honeys were to be included in clinical treatment, it would reduce the demand for antibiotic usage. In this review, we outline the constituents of honey and how they affect antibiotic potential in a clinical setting. By identifying the key components, we facilitate the development of an optimally antimicrobial honey by either synthetic or semisynthetic production methods.
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Harbo, John R. "Worker-Bee Crowding Affects Brood Production, Honey Production, and Longevity of Honey Bees (Hymenoptera: Apidae)." Journal of Economic Entomology 86, no. 6 (December 1, 1993): 1672–78. http://dx.doi.org/10.1093/jee/86.6.1672.

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Gerginova, Dessislava, Svetlana Simova, Milena Popova, Marina Stefova, Jasmina Petreska Stanoeva, and Vassya Bankova. "NMR Profiling of North Macedonian and Bulgarian Honeys for Detection of Botanical and Geographical Origin." Molecules 25, no. 20 (October 14, 2020): 4687. http://dx.doi.org/10.3390/molecules25204687.

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Bulgaria and North Macedonia have a long history of the production and use of honey; however, there is an obvious lack of systematic and in-depth research on honey from both countries. The oak honeydew honey is of particular interest, as it is highly valued by consumers because of its health benefits. The aim of this study was to characterize honeydew and floral honeys from Bulgaria and North Macedonia based on their NMR profiles. The 1D and 2D 1H and 13C-NMR spectra were measured of 16 North Macedonian and 22 Bulgarian honey samples. A total of 25 individual substances were identified, including quinovose, which was found for the first time in honey. Chemometric methods (PCA—principal component analysis, PLS-DA—partial least squares discriminant analysis, ANOVA—analysis of variance) were used to detect similarities and differences between samples, as well as to determine their botanical and geographical origin. Semiquantitative data on individual sugars and some other constituents were obtained, which allowed for the reliable classification of honey samples by botanical and geographical origin, based on chemometric approaches. The results enabled us to distinguish oak honeydew honey from other honey types, and to determine the country of origin. NMR was a rapid and convenient method, avoiding the need for other more time-consuming analytical techniques.
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Kulykovets, Olena, Maria Jeznach, Małgorzata Kosicka-Gębska, and Anna Kudlińska-Chylak. "Kształtowanie się rynku miodu w krajach Unii Europejskiej w latach 2001–2013." Zeszyty Naukowe SGGW - Ekonomika i Organizacja Gospodarki Żywnościowej, no. 120 (December 29, 2017): 111–20. http://dx.doi.org/10.22630/eiogz.2017.120.42.

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The article presents changes in honey production in the countries of EU in years 2001–2013. The method used in the article divided honey producers by size of the production: large, medium and small. Honey production in the group of small producers was the most dynamically growing, where the average annual growth rate was 2.93% in the considered period. Identified large volatility of honey production in the EU, which indicates the uncertainty and strong reactions of producers to the market situation. Therefore it is very important to support apiculture programs as contribution to the increasing of honey production and consumption in the respective countries.
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Triyono, Diah Rina Kamardiani, and Muhammad Adhi Prasetio. "Alocative Efficiency of Honey Pineapple Farm in Pemalang Regency, Central Java, Indonesia." E3S Web of Conferences 232 (2021): 01016. http://dx.doi.org/10.1051/e3sconf/202123201016.

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Pineapple Honey is one of the superior commodity of Pemalang Regency, Central Java. However, production of honey pineapple in 2013- 2015 was occurred fluctuations. The purpose of this research is to know the factors that affect production and to know the level of elasticity in each honey pineapple farming input in the Beluk Village. The sampling of locations was carried out purposively with the consideration that beluk village was the centre of honey pineapple production. The number of samples was 64 farmers who were taken simple random. Cobb-Douglas production function analysis technique was used to determine the factors affecting the production and elasticity of each of the honey pineapple farming inputs. The results of the analysis showed that the factors affecting the production of honey pineapple are the number of plants, manure, and harvest season. In variables that affect production is obtained only variable manure has negative value. It can be occurred as effect of the application of immature manure. So, we suggest that the use of manure that is ripe and of good quality will have a positive effect on the growth and production of honey pineapple.
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Budhathoki-Chhetri, Pratibha, Srawan Kumar Sah, Mahesh Regmi, and Sabitri Baral. "Economic analysis and marketing system of Apis mellifera honey production in Dang, Nepal." Journal of Agriculture and Natural Resources 4, no. 1 (January 1, 2021): 154–64. http://dx.doi.org/10.3126/janr.v4i1.33249.

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Dang valley, the major honey producing district in Nepal, contributes 14 percent of national honey production in the country. Understanding the constraints and opportunities contributes in improving production and productivity of honey bee (Apis mellifera), in Dang, the study was initiated to find out the status, constraints and opportunities of honey production and its marketing system in the valley so as to increase the productivity and effective marketing. Total 60 beekeepers, 35 from Ghorahi and 25 from Tulsipur sub-metropolitan municipality having more than 20 beehives were selected based on proportionate stratified random sampling method and 2 processor cum wholesalers, 2 retailers, 2 middlemen and 2 cooperatives were selected based on simple random sampling method for interview. Personal interview, focus group discussion, key informant survey was used to collect primary data and secondary data were collected from topic related publications of various institution. The average annual honey productivity was 23.5 kg/hive with benefit cost ratio of 2.15 in 2019/20. Producers disposed their honey through nine marketing channels. Out of nine honey marketing channels, maximum portion i.e. 54.14% of honey disposed through producers to processor cum wholesalers to retailers/ traders inside or outside Dang to consumers, and only 2.66% of honey disposed through producers to cooperatives to consumers. Strengthening the appropriate management practice, quality testing and product certification of honey is must to enhance production and marketing of honey.
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Lysenko, H. L., V. G. Prudnikov, A. L. Leppa, I. M. Heyda, and K. D. Buchkovska. "The production cremated honey from natural honey of different botanical origin." VETERINARY SCIENCE, TECHNOLOGIES OF ANIMAL HUSBANDRY AND NATURE MANAGEMENT, no. 3 (2019): 22–28. http://dx.doi.org/10.31890/vttp.2019.03.04.

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Costa Maia, F., D. Lourenco, S. Tsuruta, and E. Martins. "303 Selection criteria for improving honey production in Africanized honey bees." Journal of Animal Science 96, suppl_3 (December 2018): 115. http://dx.doi.org/10.1093/jas/sky404.252.

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Eischen, F. A., D. Cardoso-Tamez, W. T. Wilson, and A. Dietz. "Honey production of honey bee colonies infested with Acarapis woodi (Rennie)." Apidologie 20, no. 1 (1989): 1–8. http://dx.doi.org/10.1051/apido:19890101.

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Spivak, M., S. Batra, F. Segreda, A. L. Castro, and W. Ramírez. "Honey production by Africanized and European honey bees in Costa Rica." Apidologie 20, no. 3 (1989): 207–20. http://dx.doi.org/10.1051/apido:19890303.

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Eaton, Leonard J., and Vilis O. Nams. "Honey bee stocking numbers and wild blueberry production in Nova Scotia." Canadian Journal of Plant Science 92, no. 7 (November 2012): 1305–10. http://dx.doi.org/10.4141/cjps2012-045.

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Eaton, L. J. and Nams, V. O. 2012. Honey bee stocking numbers and wild blueberry production in Nova Scotia. Can. J. Plant Sci. 92: 1305–1310. Wild blueberries (Vaccinium angustifolium Ait.) require cross pollination by insects. Introduction of managed species such as honey bees (Apis mellifera L.) and alfalfa leafcutting bees (Megachile rotundata Fabr.) is costly. We assessed the effects of stocking rates of honey bee hives and the interacting effects of the numbers of honey bees and other bees on yield of blueberries in commercial fields. Blueberry fields were sampled from 101 fields in years 1991 to 2010 in Nova Scotia. We recorded field size, numbers of beehives, yield, densities of bees, numbers of buds, blossoms and set fruit. Yields increased linearly with numbers of beehives, up to∼4 hives/hectare, but at higher stocking rates there was too much variation to adequately determine the effects. Yields also increased linearly with numbers of honey bees, but there was an interaction with other bees that decreased the effects of honey bees, such that at maximum densities of other bees, there was no effect of honey bees on yield. These results suggest that other bees and honey bees compete for pollination. If producers have limited numbers of beehives, we suggest that more should be placed in areas where densities of other bees are lower, up to approximately 4 hives/hectare.
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Horton, Melissa A., Randy Oliver, and Irene L. Newton. "No apparent correlation between honey bee forager gut microbiota and honey production." PeerJ 3 (October 13, 2015): e1329. http://dx.doi.org/10.7717/peerj.1329.

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One of the best indicators of colony health for the European honey bee (Apis mellifera) is its performance in the production of honey. Recent research into the microbial communities naturally populating the bee gut raise the question as to whether there is a correlation between microbial community structure and colony productivity. In this work, we used 16S rRNA amplicon sequencing to explore the microbial composition associated with forager bees from honey bee colonies producing large amounts of surplus honey (productive) and compared them to colonies producing less (unproductive). As supported by previous work, the honey bee microbiome was found to be dominated by three major phyla: the Proteobacteria, Bacilli and Actinobacteria, within which we found a total of 23 different bacterial genera, including known “core” honey bee microbiome members. Using discriminant function analysis and correlation-based network analysis, we identified highly abundant members (such asFrischellaandGilliamella) as important in shaping the bacterial community; libraries from colonies with high quantities of theseOrbaceaemembers were also likely to contain fewerBifidobacteriaandLactobacillusspecies (such as Firm-4). However, co-culture assays, using isolates from these major clades, were unable to confirm any antagonistic interaction betweenGilliamellaand honey bee gut bacteria. Our results suggest that honey bee colony productivity is associated with increased bacterial diversity, although this mechanism behind this correlation has yet to be determined. Our results also suggest researchers should not base inferences of bacterial interactions solely on correlations found using sequencing. Instead, we suggest that depth of sequencing and library size can dramatically influencestatistically significantresults from sequence analysis of amplicons and should be cautiously interpreted.
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Gašić, Uroš, Branko Šikoparija, Tomislav Tosti, Jelena Trifković, Dušanka Milojković-Opsenica, Maja Natić, and Živoslav Tešić. "Phytochemical Fingerprints of Lime Honey Collected in Serbia." Journal of AOAC INTERNATIONAL 97, no. 5 (September 1, 2014): 1259–67. http://dx.doi.org/10.5740/jaoacint.sgegasic.

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Abstract Composition of phenolic compounds and the sugar content were determined as the basis for characterization of lime honey from Serbia. Particular attention was given to differences in phytochemical profiles of ripe and unripe lime honey and lime tree nectar. Melissopalynological analysis confirmed domination of Tilia nectar in all analyzed samples. Phenolic acids, abscisic acid, flavonoids, and flavonoid glycosides were determined by means of ultra-HPLC coupled with a hybrid mass spectrometer (UHPLC–OrbiTrap). Sugar content was determined using high-performance anion-exchange chromatography with amperometric detection. Similar phenolic compounds characterized unripe and ripe honeys, while the lime tree nectar profile showed notable differences. Compared to lime tree nectar, a high amount of chrysin, pinocembrin, and galangin were detected in both ripe and unripe lime honey. Fructose and glucose were the major constituents of all investigated samples, and amounts were within the limits established by European Union legislation. Sucrose content in the nectar sample was up to two-fold higher when compared to all honey samples. Isomaltose and gentiobiose with turanose content were different in analyzed production stages of lime honey.
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Ghounem,, Sh. "HONEY PRODUCTION ECONOMICS IN GHARBAI GOVERNORATE." Journal of Agricultural Economics and Social Sciences 29, no. 9 (September 1, 2004): 5059–75. http://dx.doi.org/10.21608/jaess.2004.209800.

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Legesse Yadeta, Gemechis. "Honey Production and Marketing in Ethiopian." American Journal of Life Sciences 3, no. 1 (2015): 42. http://dx.doi.org/10.11648/j.ajls.20150301.18.

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42

Shuel, R. W. "Improving Honey Production through Plant Breeding." Bee World 70, no. 1 (January 1989): 36–45. http://dx.doi.org/10.1080/0005772x.1989.11098982.

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43

Ivanovic, Jelena, Milan Baltic, Dalibor Jelic, Jelena Janjic, Marija Boskovic, Radmila Markovic, and Marija Dokmanovic-Starcevic. "Research of produstion volume and market turnover of honey from 2004. to 2014." Veterinarski glasnik 69, no. 5-6 (2015): 467–78. http://dx.doi.org/10.2298/vetgl1506467i.

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The objective of this work was to investigate the scope of production, export and import of honey in the Republic of Serbia from 2004. to 2014. The results are based on the report of the Statistical Office of the Republic of Serbia. The observed period is characterized primarily by the increase in the number of beehives as well as an incresed honey production, followed by honey price growth and minor changes honey yield per hive. In this period, honey production ranged from 2,561 tons (in 2008) to 8,554 tons (in 2013). The largest volume of honey exports was in 2013. (3,367.7 tons, worth 14,881.4 thousand USD), and the largest import of honey into Serbia was in the same year (60.6 tons, worth 240.3 thousand USD). In the monitored period, Germany imported the largest quantities of honey from Serbia, about 4,611.0 tons (worth about 19,546.9 thousand USD), and somewhat smaller amounts of honey were imported by Italy and Montenegro. On the other hand, in the same period, Serbia imported the largest quantities of honey from the Republic of Macedonia, about 40.6 tons, worth 119.5 thousand USD. Beekeeping in Serbia definitely has a great development potential, so accordingly, its production should be encouraged in the future.
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44

Nikolić, Marija, Nebojša Nedić, and Suzana Đorđević-Milošević. "Cost-effectiveness analysis of organic honey production in Serbia." Ekonomika poljoprivrede 69, no. 2 (2022): 533–47. http://dx.doi.org/10.5937/ekopolj2202533n.

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Economic justification of organic honey production is analyzed in the paper. The emphasis was placed on specific revenues and expenses that occur in organic honey production, and economic justification was examined by calculating indicators of economic efficiency, profitability and labour productivity. Based on the collected data, a calculation of production was made and a profitability threshold was established, which is achieved with 38 bee colonies, or with production volume of 570 kg of honey. Farms with more colonies have better indicators of production success. The expenses arising from the transition from conventional to organic production will be most quickly covered by farms with 200 bee colonies that generate enough income from organic honey production to operate positively in the third year of organizing such production.
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45

Guan, Wenjing, Xin Zhao, Danielle D. Treadwell, Michael R. Alligood, Donald J. Huber, and Nicholas S. Dufault. "Specialty Melon Cultivar Evaluation under Organic and Conventional Production in Florida." HortTechnology 23, no. 6 (December 2013): 905–12. http://dx.doi.org/10.21273/horttech.23.6.905.

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Interest in producing specialty melons (Cucumis melo) is increasing in Florida, but information on yield performance, fruit quality, and disease resistance of specialty melon cultivars grown in Florida conditions is limited. In this study conducted at Citra, FL, during the 2011 Spring season, 10 specialty melon cultivars were evaluated, in both certified organic and conventionally managed fields, including: Creme de la Creme and San Juan ananas melon (C. melo var. reticulatus), Brilliant and Camposol canary melon (C. melo var. inodorus), Ginkaku and Sun Jewel asian melon (C. melo var. makuwa), Arava and Diplomat galia melon (C. melo var. reticulatus), and Honey Pearl and Honey Yellow honeydew melon (C. melo var. inodorus). ‘Athena’ cantaloupe (C. melo var. reticulatus) was included as a control. ‘Sun Jewel’, ‘Diplomat’, ‘Honey Yellow’, and ‘Honey Pearl’ were early maturing cultivars that were harvested 10 days earlier than ‘Athena’. ‘Athena’ had the highest marketable yield in the conventional field (10.7 kg/plant), but the yield of ‘Camposol’, ‘Ginkaku’, ‘Honey Yellow’, and ‘Honey Pearl’ did not differ significantly from ‘Athena’. Under organic production, ‘Camposol’ showed a significantly higher marketable yield (8.3 kg/plant) than ‘Athena’ (6.8 kg/plant). ‘Ginkaku’ produced the largest fruit number per plant in both organic (10 fruit/plant) and conventional fields (12 fruit/plant) with smaller fruit size compared with other melon cultivars. Overall, the specialty melon cultivars, except for asian melon, did not differ significantly from ‘Athena’ in terms of marketable fruit number per plant. ‘Sun Jewel’, ‘Diplomat’, and ‘San Juan’ showed relatively high percentages of cull fruit. ‘Honey Yellow’, ‘Honey Pearl’, and ‘Sun Jewel’ exhibited higher soluble solids concentration (SSC) than ‘Athena’ in both organic and conventional fields, while ‘Brilliant’, ‘San Juan’, and ‘Ginkaku’ also had higher SSC than ‘Athena’ under organic production. ‘Honey Yellow’, ‘Sun Jewel’, ‘Brilliant’, and ‘Camposol’ were less affected by powdery mildew (caused by Podosphaera xanthii) and downy mildew (caused by Pseudoperonospora cubensis) in the conventional field. ‘Honey Yellow’ and ‘Camposol’ also had significantly lower aboveground disease severity ratings in the organic field compared with ‘Athena’, although the root-knot nematode (RKN) (Meloidogyne sp.) gall rating was higher in ‘Honey Yellow’ than ‘Athena’.
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46

Beula, K., and A. Anandhy. "A Study on Honey Production and Marketing by the Marthandam Beekeepers’ Co-Operative Society in Kanniyakumari District." Shanlax International Journal of Economics 8, no. 2 (March 1, 2020): 33–40. http://dx.doi.org/10.34293/economics.v8i2.1632.

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In this paper, we explain honey production and marketing of the Marthandam Beekeepers Co-Operative Society in Kanniyakumari District. Honey is a sweet, liquid produced by honey bees from nectars and other sweet substances of plants by the addition of their digestive enzymes and by ripening the resulting mixture. The variety of honey produced by honey bees is the best known, due to its worldwide commercial production and human consumption. Data for the present study has been collected from primary sources of information. Hence, the Martandam Bee-keepers Co-operative Society, which faces production and marketing problems, should be rejuvenated and refurbished.
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Hossain, Mohammed Sakhawat, Md Mohi Uddin Fazlullah, and Md Mizanur Rahman. "Comparative Performance of Honey Production from Two Different Bee Hives in Bangladesh." Agriculturists 17, no. 1-2 (December 26, 2019): 102–11. http://dx.doi.org/10.3329/agric.v17i1-2.44700.

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The study was conducted in three districts viz., Sirajgonj, Gazipur and Satkhira during the mustard, litchi, and mangrove blooming period, respectively during 2015-2016 and 2016-2017 cropping seasons to study honey production efficiency of traditional hive and poly super hive. The study was carried out in paired plot design. Fourteen boxes were used in each setup, among them seven were traditional hive and seven were poly super hive. In Ullapara of Sirajganj district during 2015-16 poly super hive yielded the highest (17.03 kg hive-1season-1) honey. In 2016-17 (mustard) the highest honey was harvested from poly super hive operated at Tarash (18.63 kg hive-1season-1) and the lowest honey yielded in poly super hive at Shahjadpur. At Kapasia of Gazipur during 2015-2016 (litchi) the poly super hive produced the highest (20.46 kg hive-1season-1) honey yield cropping season and the lowest (20 kg hive-1season-1) was in Kaliganj (Gazipur district). On the other hand, during 2016-2017 it was observed that in poly super hive produced the highest (14.92 kg hive-1season-1) honey operated in Gazipur Sadar and the lowest (13.93 kg hive-1season-1) was in Kaliganj. In Satkhira during 2015-16 cropping season (mangrove) the poly super hive gave the highest (14.92 kg hive-1season-1) honey yield and it was obtained from Tala and the lowest (14.65 kg hive-1season-1) honey was yielded and it obtained in Kaliganj. Again during cropping season 2016-17 poly super hive produced the highest (12.5 kg hive-1season-1) honey and it was from Tala and the lowest (11.45 kg hive-1season-1) in Munshiganj. There was significantly less honey production was recorded in traditional bee hives than the poly super hive. During 2016-17 season honey production was higher than 2015-16 season at Sirajganj. However, opposite result was observed at Gazipur and Satkhira. The Agriculturists 2019; 17(1-2) 102-111
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48

Bredikhin, Sergey, Alexander Martekha, Vladimir Andreev, Yuliya Kaverina, and Igor Korotkiy. "Rheological Properties of Mayonnaise with Non-Traditional Ingredients." Food Processing: Techniques and Technology 52, no. 4 (December 21, 2022): 739–49. http://dx.doi.org/10.21603/2074-9414-2022-4-2402.

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Rheological measurements are used in the food industry to determine physical characteristics of raw materials, as well as semi-finished and finished products. We aimed to study the effects of ingredients and homogenization parameters on the rheological properties of mayonnaise prepared with pumpkin and rice oils, as well as various honeys. Mayonnaise samples were prepared with non-traditional ingredients, namely cold-pressed pumpkin seed oil, refined rice oil, and four varieties of honey (acacia, linden, forest, and spring). The samples were made in the traditional way on an Ultra Turrax T25 IKA homogenizer (3500–24 000 rpm). The rheological properties of honey and mayonnaise were determined on a Brookfield rotational viscometer. Forest honey had the highest viscosity, while linden honey had the lowest viscosity, compared to the other honeys. The sample of mayonnaise with forest honey had the highest effective viscosity (3.427 Pa·s) and consistency (101.26 Pa·sn). The use of whey powder provided mayonnaise with the most optimal rheological parameters. Of all carbohydrates, inulin HD had the best effect on the consistency of mayonnaise, with effective viscosity of 2.801 ± 0.001 Pa·s and a flow index of 0.2630 ± 0.0020. Disaccharides provided mayonnaise with higher viscosity and consistency than monosaccharides. Mayonnaise with fresh egg yolk had higher viscosity (2.656 ± 0.002 Pa·s) and consistency (65.640 ± 0.004 Pa·s) than the samples with other egg products. The rheological characteristics of mayonnaise were also determined by the homogenization time and rotor speed. Increasing the time from 2 to 4 min at 10 000 rpm raised the emulsion’s viscosity and consistency from 6.253 to 8.736 Pa·s and from 77.42 to 134.24 Pa·sn, respectively, as well as reduced the flow index from 0.2628 to 0.1995. The rotor speed of 10 000–12 000 rpm was optimal for mayonnaise with pumpkin and rice oils and honey. The studied samples of mayonnaise with pumpkin and rice oils, as well as honey, belong to non-Newtonian systems and pseudoplastic fluids. The empirical flow curves can be adequately described by the Herschel-Bulkley model. Our results can significantly increase the efficiency of mayonnaise production, improve its quality, and reduce production costs.
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49

Pignagnoli, Arianna, Stefano Pignedoli, Emanuele Carpana, Cecilia Costa, and Aldo Dal Prà. "Greenhouse Gas (GHG) Emissions from Honey Production: Two-Year Survey in Italian Beekeeping Farms." Animals 13, no. 4 (February 20, 2023): 766. http://dx.doi.org/10.3390/ani13040766.

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The objective of this study was to quantify the climate change (CC) impact of the honey supply chain in different beekeeping systems and farms, over two consecutive years. The CC impact category is quantified as kg CO2 equivalent and it evaluates the GHG emissions, mainly CO2, N2O, and CH4. The results ranged from 0.44 to 3.18 (p = 0.039) kg CO2e/kg honey with higher values in 2021 than 2020. The main contributors to climate change of the honey supply chain are represented by transport and supplemental feeding inputs. The beekeeping system (migratory or stationary) influenced CC: the contribution to CC for stationary farms was estimated at 0.58 kg CO2e/kg honey and 2.48 for migratory ones (p < 0.001). Given the close connection between honey yield and LCA results due to the unit of measurement of impact, i.e., kg of honey produced, an index was developed (wildflower honey climate index) as a simple benchmark tool for prediction of honey yield in the survey context. Using the data from the present study, we found that the index is positively related to honey yield (r = 0.504; p < 0.05) but negatively related to supplemental feeding (r = −0.918; p < 0.01) and overall carbon footprint (r = −0.657; p < 0.05). Further studies are needed to better explain the effects of weather on honey production, as well as environmental impact.
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50

Manning, R. "Evaluation of the Western Australian queen bee breeding program." Australian Journal of Experimental Agriculture 36, no. 4 (1996): 513. http://dx.doi.org/10.1071/ea9960513.

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Agriculture Western Australia's former Queen Bee Breeding Program (QBBP) was successful in significantly raising the productivity of honey bee colonies for those beekeepers who regularly purchased queen bees from the program. The increased productivity was measured through honey production. Over 2 nectar flows, colonies headed by queens purchased from the breeding program (group 2) showed a 35% increase in honey production over unselected colonies (group 1-not purchased from the QBBP). Over individual nectar flows, colonies headed by queens purchased from the breeding program showed a 22% increase in honey production from a Eucalyptus calophylla nectar flow, and for a second protracted winter nectar flow from coastal heath they also produced 46% more honey than unselected queen bees. Honey production (kg/colony.day) from Eucalyptus calophylla was 0.95 and 1.13 kg for groups 1 and 2, respectively. Honey production from the coastal heath was 0.27 and 0.4 kg/colony.day for groups 1 and 2, respectively. Results were projected for 1 year and scaled up to represent a commercial apiary of 400 colonies. According to estimates from the data, group 2 beekeepers should have benefited from the QBBP by being able to produce 24 t of honey from their apiaries, valued in excess of $A32 000, more than group 1 beekeepers.
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