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Dissertations / Theses on the topic 'Hormone immunoassays'

Author: Grafiati

Published: 5 June 2025

Last updated: 16 July 2025

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1

Rauf, Femina. "Chimeric and Recombinant Protein Reagents for Cellular Analysis and Immunoassays." Diss., The University of Arizona, 2011. http://hdl.handle.net/10150/145441.

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Development of chimeric, recombinant peptides, proteins and enzymes expands the availability of protein/enzyme–based tools for cellular analysis and new assay platforms. Ideal protein reagents for cellular analysis must translocate into a variety of cells with minimum cell damage, retain stability and biological activity within the cell during analysis, and provide a reliable, measurable signal. This work focused on development, characterization and utilization of chimeric recombinant peptide, protein and enzyme reagents for cellular analysis and immunoassays. A cell-penetrating, fluorescent p

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2

Casagrande, Alessandra. "Importância dos ensaios para medida do hormônio de crescimento na determinação da atividade da acromegalia." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2005. http://hdl.handle.net/10183/7598.

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A dosagem do GH no soro é essencial para confirmar ou excluir o seu excesso. Na acromegalia, a ausência de critérios clínicos suficientemente sensíveis para monitorizar o sucesso do tratamento faz com que o GH sérico seja o procedimento de escolha e, para isso, é essencial que a sua dosagem seja realizada de forma confiável, capaz de permitir interpretações uniformes. Vários critérios hormonais têm sido propostos para caracterizar remissão da acromegalia, incluindo níveis séricos de GH randômico inferior a 2,5 μg/l, nadir de GH durante o teste de tolerância oral a glicose inferior a 1,0 μg/l e

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3

El, Fighi Abdul Baset Abdussalam M. "Studies on the immunoassay of human growth hormone in urine." Thesis, University of Salford, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.244870.

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4

Evans, Lee W. "The development of immunoassays for follistatin and activin and their clinical applications." Thesis, University of Reading, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.389532.

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5

Ritter, Nina. "Detection of Anti-hGH Antibodies in Serum Samples of Children Treated with RhGH." Doctoral thesis, Universitätsbibliothek Leipzig, 2012. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-97443.

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The present study deals with the comparison and establishment of methods for the detection of antibodies against recombinant human growth hormone (rhGH). Therefore, different methods for the detection of hGH-Abs were evaluated and compared in order to establish a test system that can be used for the detection of neutralizing antibodies against hGH, which could be developed under rhGH treatment. This manuscript describes in detail the validation of a newly developed biological assay, the neutralizing hGH-antibody assay (NAb assay). Therefore, a cell line transfected with the growth hormone rece

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6

Maioli, Marcos Antonio [UNESP]. "Quantificação do fator de crescimento semelhante a insulina I (IGF-I) em plasma bovino por ELISA." Universidade Estadual Paulista (UNESP), 2016. http://hdl.handle.net/11449/134284.

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Submitted by MARCOS ANTONIO MAIOLI null (maioli_marcos@hotmail.com) on 2016-02-17T13:03:36Z No. of bitstreams: 1 Tese Maioli, M.A..pdf: 2279252 bytes, checksum: 484c7c928f7a1a221c494bcff9e436b0 (MD5)<br>Approved for entry into archive by Juliano Benedito Ferreira (julianoferreira@reitoria.unesp.br) on 2016-02-17T16:54:14Z (GMT) No. of bitstreams: 1 maioli_ma_dr_araca.pdf: 2279252 bytes, checksum: 484c7c928f7a1a221c494bcff9e436b0 (MD5)<br>Made available in DSpace on 2016-02-17T16:54:14Z (GMT). No. of bitstreams: 1 maioli_ma_dr_araca.pdf: 2279252 bytes, checksum: 484c7c928f7a1a221c494bcff9e4

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Maioli, Marcos Antonio. "Quantificação do fator de crescimento semelhante a insulina I (IGF-I) em plasma bovino por ELISA /." Araçatuba, 2016. http://hdl.handle.net/11449/134284.

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Orientador: Guilherme de Paula Nogueira<br>Banca: Marcelo Vasconcelo Meireles<br>Banca: Daniel de Jesus Cardoso de Oliveira<br>Banca: Andréa Fontes Garcia<br>Resumo: Esse estudo teve como objetivo a padronização de um ensaio imunoenzimático (ELISA) para a determinação das concentrações plasmáticas de IGF-I total, utilizando o sistema de amplificação biotina-estreptavidina peroxidase em um ensaio competitivo. O IGF-I foi extraído da IGFBP, utilizando o tampão glicina acidificado seguido de neutralização do pH com hidróxido de sódio. As microplacas foram sensibilizadas com anti IgG de coelho, e

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8

Ramchandra, Desai Suchita. "Development of an immunoassay panel to predict aseptic implant loosening." Thesis, Högskolan i Skövde, Institutionen för biovetenskap, 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-16285.

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During our lifetime, our bones constantly go through remodelling to maintain the skeletal system. This is done by osteoblasts that deposit new bone tissue, osteoclasts that remove the bone matrix and mechanosensing osteocytes. In case of bone implants, increased resorption by osteoclasts due to inflammation (inflammatory osteolysis) leads to aseptic implant loosening. This study focuses on how to detect these inflammatory resorbing cells at an early stage and prevent their activity with appropriate medication. To achieve this, we differentiated classical monocytes into macrophage-like cells, o

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9

Dowd, Peter Dylan. "The photophysical characterisation of a fluorescence-based immunoassay for the detection of gonadotropin-releasing hormone, type-1 (GnRH-I)." Thesis, University of Strathclyde, 2013. http://oleg.lib.strath.ac.uk:80/R/?func=dbin-jump-full&object_id=24378.

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The homogeneous assay format has been identified as having the potential to make an effective impact in the field of 'point-of-care or near patient testing. Homogeneous assays have the advantage that, by eliminating the need for multiple preparation steps, they can be rapid and easy to use in comparison with most solid-phase assay formats. However homogeneous assays tend to be generally less sensitive than their heterogeneous counterparts, giving results that are qualitative or at best semi-quantitative. This work presents a 'model' fluorescence-based homogeneous immunoassay for the detection

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10

Polegato, Bruna Furlan. "Determinação dos perfis de estrógenos e progestinas fecais durante o ciclo estral, gestação e período pós-parto em cervos-do-pantanal (Blastocerus dichotomus) em cativeiro /." Jaboticabal : [s.n.], 2008. http://hdl.handle.net/11449/98204.

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Orientador: José Maurício Barbanti Duarte<br>Banca: Paulo Henrique Franceschini<br>Banca: Claudio Alvarenga de Oliveira<br>Resumo: Atualmente, o conhecimento sobre a fisiologia e a biologia reprodutiva de cervos-do-pantanal (Blastocerus dichotomus) é escasso e as lacunas existentes aumentam a vulnerabilidade da espécie à extinção. Neste contexto, os objetivos deste trabalho foram: (1) caracterizar alguns parâmetros relacionados à fisiologia reprodutiva de fêmeas de cervos-do-pantanal e (2) fornecer métodos adequados para o acesso às diferentes fases do ciclo reprodutivo. Para isso, seis fêmeas

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Polegato, Bruna Furlan [UNESP]. "Determinação dos perfis de estrógenos e progestinas fecais durante o ciclo estral, gestação e período pós-parto em cervos-do-pantanal (Blastocerus dichotomus) em cativeiro." Universidade Estadual Paulista (UNESP), 2008. http://hdl.handle.net/11449/98204.

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Made available in DSpace on 2014-06-11T19:29:16Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-08-08Bitstream added on 2014-06-13T19:17:43Z : No. of bitstreams: 1 polegato_bf_me_jabo.pdf: 388236 bytes, checksum: 27d5324f7a537c0e242c14342ba2b294 (MD5)<br>Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)<br>Atualmente, o conhecimento sobre a fisiologia e a biologia reprodutiva de cervos-do-pantanal (Blastocerus dichotomus) é escasso e as lacunas existentes aumentam a vulnerabilidade da espécie à extinção. Neste contexto, os objetivos deste trabalho foram: (1) caracterizar a

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12

Lehmphul, Ina. "Zelluläre Wirkung, Wirkmechanismen und Nachweisverfahren von Schilddrüsenhormonen und ihren Metaboliten." Doctoral thesis, Humboldt-Universität zu Berlin, Lebenswissenschaftliche Fakultät, 2015. http://dx.doi.org/10.18452/17434.

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Schilddrüsenhormone (TH) regulieren Metabolismus und Energiestoffwechsel. Der TH‐Metabolit (THM) 3,5‐T2 (3,5‐Diiod‐L‐Thyronin) aktiviert Fett‐Oxidation und mitochondriale Atmung. Der THM 3‐Iodothyronamin (3‐T1AM) beeinflusst zusätzlich glukoregulatorische Prozesse. THM können zur Reduktion von Körperfett beitragen. Um 3,5‐T2 im humanen Serum nachzuweisen sollte ein Immunoassay aufgebaut, validiert und angewendet werden. In intakten hepatozellulären (HepG2) sowie pankreatischen ß‐Zellen (MIN6) sollte untersucht werden ob THM durch Modulation der mitochondrialen Aktivität die zelluläre Substra

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13

Such, Sanmartin Gerard. "Assessing human growth hormone variants to determine their potential relevance in anti-doping and clinical analysis." Doctoral thesis, Universitat Pompeu Fabra, 2010. http://hdl.handle.net/10803/7198.

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Human growth hormone (GH) participates in human longitudinal growth, lipid and carbohydrate metabolism, and comprises a remarkably number of proteins with similar sequences, generated either genetically or post-translationally, that in some cases show clearly differentiated biological activities. Current methods employed for its quantification are mainly based on a specific immunodetection of the most concentrated GH variants in blood circulation. However, it is not clear neither which variants are recognised in each case, nor which is the real concentration of some of these variants. Probably

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14

Woloszynek, Renata dos Santos Batista Reis. "Validação, valores normativos e aplicabilidade clínica de um ensaio imunoenzimático para determinação sérica do hormônio anti-Mülleriano." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/5/5135/tde-11082014-120509/.

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O hormônio anti-Mülleriano (AMH) é um marcador de reserva ovariana e função testicular. A aplicação clínica desse hormônio requer padronização adequada de valores de referência, de acordo com o imunoensaio utilizado. Os objetivos deste estudo foram: validar o imunoensaio AMH Gen II (Beckman Coulter Company, TX, USA), estabelecer valores normais de AMH em homens e mulheres saudáveis, avaliar a influência do uso de contraceptivos hormonais, tabagismo e índice de massa corpórea (IMC) sobre os valores de AMH e verificar as concentrações séricas desse hormônio em pacientes com síndrome de Turner (S

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15

Höfig, Carolin. "Establishment, validation and application of immunological and LC-MS/MS-based detection methods to study the role of human aromatic L-amino acid decarboxylase as an enzyme potentially involved in thyronamine biosynthesis." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2012. http://dx.doi.org/10.18452/16645.

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Thyronamine (TAM) sind eine neue Molekülklasse, die endokrinologische und metabolische Prozesse miteinander vereinen. Der biologisch aktive Metabolit 3-Iod-L-Thyronamin (3-T1AM) wird durch eine kombinierte Deiodierung und Decarboxylierung von Schilddrüsenhormonen (TH) gebildet. Existierende Methoden zum Nachweis und zur Quantifizierung von 3-T1AM im menschlichen Serum sind immer noch umstritten. Auch die an der Biosynthese vermutlich beteiligte TH-Decarboxylase konnte noch nicht identifiziert werden. Für die Identifizierung und Quantifizierung von TH und TAM Profilen wurde die Flüssigchromatog

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16

Abellán, Sánchez María Rosario. "Estudio en poblaciones seleccionadas de la fiabilidad de nuevos protocolos de detección de consumo de hormonas recombinantes (hgH y EPO)." Doctoral thesis, Universitat Pompeu Fabra, 2006. http://hdl.handle.net/10803/7103.

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Las hormonas recombinantes eritropoyetina (EPO) y hormona de crecimiento (GH), prácticamente iguales a las endógenas y de corta vida media en circulación, son de difícil detección directa en el control antidopaje. <br/>Se determinaron los valores poblacionales de los biomarcadores indirectos EPO, receptor soluble de la transferrina, insulin-like growth factor-I (IGF-I) y procolágeno tipo III péptido (P-III-P), en poblaciones seleccionadas de deportistas, y el efecto del ejercicio y los distintos tipos de entrenamiento sobre su concentración sérica. <br/>La comparación de resultados obtenidos m

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17

Tien, Chia-ying, and 田家穎. "Development of Liposomal Immunoassays for the Detection of Progesterone and Luteinizing Hormone." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/27853287229145513602.

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碩士<br>國立清華大學<br>化學系<br>98<br>There are two parts of work included in this study. The first part of work deals with the fabrication of immunosensors for the detection of progesterone, based on a liposomal competitive assay. Two different sensing platforms were presented herein for the development of the progesterone sensors, one is nitrocellulose membrane-based immunochromatographic approach with optical density measurement; and the other is screen-printed electrode strip with electrochemical detection. Immobilized anti-progesterone antibody on the detection surface, the progesterone-modified l

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18

Matušková, Lucie. "Neinvazivní měření steroidních hormonů a vliv hormonální manipulace na chování gekona Paroedura picta." Master's thesis, 2011. http://www.nusl.cz/ntk/nusl-312754.

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Hormones influence life of all animals. Not only they affect physiological changes in organisms, but also impact their behaviour. This work focuses at two main groups of steroid hormones: glucocorticoids and androgens. Glucocortiods are activated in response to stress. Their levels can be measured using non-invasive methods, which have a range of advantages. The main advantage is the feedback-free sample collection for enzyme immunoassay. As the measurement involves metabolites of the hormones rather than the hormones themselves, prior validation of the method is, however, necessary. This work

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19

Stähle, Daniel [Verfasser]. "Validierung eines Immunoassays für Inhibin B im Serum und Korrelation der Meßwerte mit allelischen Varianten des Follikelstimulierenden-Hormon-Rezeptors bei fertilen und infertilen Männern / vorgelegt von: Daniel Stähle." 2004. http://d-nb.info/97214305X/34.

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20

Schön, Liligret Valerie. "Etablierung, Validierung und Anwendung einer gaschromatographisch-massenspektrometrischen Methode zur Analyse von Testosteron und 17α-OH-Progesteron im Serum". Doctoral thesis, 2013. http://hdl.handle.net/11858/00-1735-0000-0001-BB45-7.

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Ziele: Die Arbeit hatte die Etablierung und Validierung einer robusten, spezifischen und sensitiven GC-NCI-MS Methode zum Ziel, um Testosteron und 17α-OH-Progesteron im Serum zuverlässig quantifizieren zu können. Anschließend wurde die Methode mit dem Siemens ADVIA Centaur Immunoassay zur Testosteronanalyse verglichen. Hintergrund der Arbeit ist die mangelnde Richtigkeit und Sensitivität der Steroidhormonanalyse mit Immunoassays, inbesondere bei Hormonanylsen in geringen Konzentrationen, wie z.B. Testosteron bei Frauen und Kindern. Methode: Die Methode umfasste die Zugabe von deuterierten Int

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