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Journal articles on the topic 'Hormone immunoassays'

Author: Grafiati

Published: 5 June 2025

Last updated: 16 July 2025

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1

Sokoll, Lori J., Frank H. Wians, and Alan T. Remaley. "Rapid Intraoperative Immunoassay of Parathyroid Hormone and Other Hormones: A New Paradigm for Point-of-Care Testing." Clinical Chemistry 50, no. 7 (2004): 1126–35. http://dx.doi.org/10.1373/clinchem.2003.030817.

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Abstract Background: The first description of the use of a rapid assay for the measurement of intact parathyroid hormone (PTH) in patients undergoing parathyroidectomy for hyperparathyroidism was reported in 1988. Subsequent improvements in the analytical performance of the rapid intraoperative PTH assay allowed the establishment of its clinical utility in the surgical management of hyperparathyroidism. These modifications also allowed the assay to be performed in or near the operating suite. Methods: We searched MEDLINE, using the following key words: intraoperative, rapid, quick, parathyroid

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Welsh, Kerry J., and Steven J. Soldin. "DIAGNOSIS OF ENDOCRINE DISEASE: How reliable are free thyroid and total T3 hormone assays?" European Journal of Endocrinology 175, no. 6 (2016): R255—R263. http://dx.doi.org/10.1530/eje-16-0193.

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Hypothyroidism is a very common disorder worldwide, for which the usual treatment is monotherapy with levothyroxine (L-T4). However, a number of patients treated with L-T4 continue to report symptoms of hypothyroidism despite seemingly normal levels of thyroid-stimulating hormone (TSH), free-T3 (FT3) and free-T4 (FT4) measured by immunoassay. This review summarizes the limitations of the immunoassays commonly used to measure thyroid hormone levels and emphasizes the advantages of the role of liquid chromatography-tandem mass spectrometry (LC-MS/MS). Immunoassays for free thyroid hormone are af

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Dewey, Hannah, Jacob Thompson, Matthew Fisher, and Januka Budhathoki-Uprety. "(Nanocarbons Division Poster Award, Third Place) Development of an Optical Probe for the Detection of Steroid Hormones." ECS Meeting Abstracts MA2024-01, no. 8 (2024): 827. http://dx.doi.org/10.1149/ma2024-018827mtgabs.

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Hormones play major roles in several biological processes. Steroid hormones are crucial for reproductive maturation in women. Abnormal steroid hormones (e.g., estrogen) levels have several pathological conditions, including ovarian failure, bone loss, or increased risk for breast cancer. Thus, accurate and precise detection of steroid hormone levels is crucial for timely health interventions. The current gold standard for steroid hormone detection is immunoassays. Optical measurement technologies are promising alternatives to traditional immunoassays. Optical tools could provide a novel platfo

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Braunstein, Glenn D. "Spurious Serum Hormone Immunoassay Results: Causes, Recognition, Management." European Endocrinology 18, no. 2 (2022): 141. http://dx.doi.org/10.17925/ee.2022.18.2.141.

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For over 50 years, immunoassays have been extensively used to quantitate hormones in blood, other fluids and tissues. Each assay has its own sensitivity, specificity and other analytical components. Despite the differences between commercial products, these assays provide important clinical information about hormone levels in patients. However, inaccurate results can occur because of technical issues, as well as patient-specific factors that can interfere with immunoassay hormone measurements. The latter include excessive normal blood or serum components, the presence of cross-reacting substan

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Kato, Hajime, Naoko Hidaka, Minae Koga, et al. "Altered Thyroid Function Tests Observed in Hypophosphatasia Patients Treated with Asfotase Alfa." International Journal of Endocrinology 2021 (October 28, 2021): 1–5. http://dx.doi.org/10.1155/2021/5492267.

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Background. Asfotase alfa is the only approved treatment that can normalize mineralization in patients with hypophosphatasia (HPP). Its interference in alkaline phosphatase (ALP) dependent immunoassays has been reported. Objective. To describe thyroid function tests interfered with by asfotase alfa and elucidate the underlying mechanism. Patients and Methods. Three patients with HPP treated with asfotase alfa were included. Thyroid hormone levels measured using five different immunoassays with or without ALP as a labeling enzyme during asfotase alfa treatment were evaluated. Results. After the

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Klee, George G. "Interferences in hormone immunoassays." Clinics in Laboratory Medicine 24, no. 1 (2004): 1–18. http://dx.doi.org/10.1016/j.cll.2004.01.003.

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Debeljak, Željko, Ivana Marković, Vatroslav Šerić, et al. "Analytical bias of automated immunoassays for six serum steroid hormones assessed by LC-MS/MS." Biochemia medica 30, no. 3 (2020): 422–31. http://dx.doi.org/10.11613/bm.2020.030701.

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Introduction: There is a growing amount of evidence showing the significant analytical bias of steroid hormone immunoassays, but large number of available immunoassays makes conduction of a single comprehensive study of this issue hardly feasible. Aim of this study was to assess the analytical bias of six heterogeneous immunoassays for serum aldosterone, cortisol, dehydroepiandrosterone sulphate (DHEAS), testosterone, 17-hydroxyprogesterone (OHP) and progesterone using the liquid chromatography coupled to the tandem mass spectrometry (LC-MS/MS). Materials and methods: This method comparison st

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Strilić, David, Bojan Stanimirov, and Maja Đanić. "Biotin interference with laboratory test results." Hospital Pharmacology - International Multidisciplinary Journal 11, no. 3 (2024): 1509–28. https://doi.org/10.5937/hpimj2403509s.

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Introduction: Biotin interference in laboratory tests is a growing concern, particularly due to the widespread use of biotin not only as a therapeutic agent but also as a supplement in the cosmetic industry. Immunoassays utilizing biotin-streptavidin technology are especially susceptible to interference, which can result in inaccurate test results, misdiagnosis, and inappropriate treatment. Methodology: This review synthesizes information from scientific literature available through databases like PubMed and Google Scholar. Keywords such as "biotin", "interference" and "immunoassay" were used

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Seth, John. "Standardization of Protein Hormone Immunoassays." Annals of Clinical Biochemistry: International Journal of Laboratory Medicine 33, no. 6 (1996): 482–85. http://dx.doi.org/10.1177/000456329603300602.

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10

Strathmann, Frederick G., Grace Borlee, Donald E. Born, Luis F. Gonzalez-Cuyar, Bertrand R. Huber, and Geoffrey S. Baird. "Multiplex Immunoassays of Peptide Hormones Extracted from Formalin-Fixed, Paraffin-Embedded Tissue Accurately Subclassify Pituitary Adenomas." Clinical Chemistry 58, no. 2 (2012): 366–74. http://dx.doi.org/10.1373/clinchem.2011.170613.

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Abstract BACKGROUND The current gold standard for diagnostic classification of many solid-tissue neoplasms is immunohistochemistry (IHC) performed on formalin-fixed, paraffin-embedded (FFPE) tissue. Although IHC is commonly used, there remain important issues related to preanalytic variability, nonstandard methods, and operator bias that may contribute to clinically significant error. To increase the quantitative accuracy and reliability of FFPE tissue–based diagnosis, we sought to develop a clinical proteomic method to characterize protein expression in pathologic tissue samples rapidly and q

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Després, Normand, and Andrew M. Grant. "Antibody interference in thyroid assays: a potential for clinical misinformation." Clinical Chemistry 44, no. 3 (1998): 440–54. http://dx.doi.org/10.1093/clinchem/44.3.440.

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Abstract Measurements of thyrotropin and of total and free thyroxine and triiodothyronine are widely used diagnostic methods for thyroid function evaluation. However, some serum samples will demonstrate a nonspecific binding with assay reagents that can interfere with the measurement of these hormones. Several recent case reports have described the presence of such interferences resulting in reported abnormal concentrations of thyroid hormones inconsistent with the patient’s thyroid state. Circulating thyroid hormone autoantibodies, described in thyroid and nonthyroid disorders, are an importa

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Bohn, Mary Kathryn, Victoria Higgins, Shervin Asgari, et al. "Paediatric reference intervals for 17 Roche cobas 8000 e602 immunoassays in the CALIPER cohort of healthy children and adolescents." Clinical Chemistry and Laboratory Medicine (CCLM) 57, no. 12 (2019): 1968–79. http://dx.doi.org/10.1515/cclm-2019-0707.

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Abstract Background The diagnostic utility of laboratory tests in paediatric medicine relies heavily on the availability of appropriate reference intervals (RIs). The Canadian Laboratory Initiative on Paediatric Reference Intervals (CALIPER) has established a comprehensive database of covariate-stratified RIs for many paediatric laboratory tests using a large, healthy reference population. Several automated analysers in widespread use in clinical laboratories have already been studied. Here, we extend the testing to Roche immunoassays and report, for the first time, comprehensive paediatric RI

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Fisker, Sanne, and Hans Ørskov. "Factors Modifying Growth Hormone Estimates in Immunoassays." Hormone Research 46, no. 4-5 (1996): 183–87. http://dx.doi.org/10.1159/000185020.

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14

Wallach, Edward E., Gregory H. Corsan, Dolly Ghazi, and Ekkehard Kemmann. "Home urinary luteinizing hormone immunoassays: clinical applications." Fertility and Sterility 53, no. 4 (1990): 591–601. http://dx.doi.org/10.1016/s0015-0282(16)53448-7.

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Bosch, A. M. G. "Enzym- und Sol Particle Immunoassays für Hormone." Archives of Gynecology and Obstetrics 242, no. 1-4 (1987): 509–12. http://dx.doi.org/10.1007/bf01783229.

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Higgins, Victoria, Angela W. S. Fung, Man Khun Chan, Joseph Macri, and Khosrow Adeli. "Pediatric reference intervals for 29 Ortho VITROS 5600 immunoassays using the CALIPER cohort of healthy children and adolescents." Clinical Chemistry and Laboratory Medicine (CCLM) 56, no. 2 (2018): 327–40. http://dx.doi.org/10.1515/cclm-2017-0349.

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Abstract Background: Accurate reference intervals (RIs) based on a healthy pediatric population are essential for pediatric test result interpretation. The CALIPER project has recruited a large healthy cohort and completed a series of a priori studies to address gaps in pediatric RIs. As immunoassays from different manufacturers for endocrine and special chemistry markers are not standardized and show marked intermethod differences, direct RI studies are needed for each major analytical platform. Here, we report age- and sex-specific pediatric RIs for 29 immunoassays on the Ortho Clinical Diag

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17

Ekins, R. "Validity of analog free thyroxin immunoassays." Clinical Chemistry 33, no. 12 (1987): 2137–44. http://dx.doi.org/10.1093/clinchem/33.12.2137.

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Abstract I have briefly illustrated the way in which the main features of analog methods may be readily predicted by consideration of elementary physicochemical laws. (Editorial limitations on the length of this presentation have prevented exploration of other issues such as the reasons for, and effects of, the hitherto unexplained inclusion by manufacturers of large amounts of albumin (12, 18) in kit reagents.) The main implication of our analysis is that, to conform genuinely to the principles of "unbound analog" free hormone immunoassay, an analog must bind to serum proteins to a maximal ex

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Kim, Ekaterina I., Diana А. Dimitrova, Diana A. Dimitrova, Nino N. Katamadze, and Tamara S. Dzantieva. "Endogenous and exogenous interferences in thyroid function immunoassays." Clinical and experimental thyroidology 16, no. 3 (2021): 16–24. http://dx.doi.org/10.14341/ket12698.

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Laboratory diagnosis of endocrine diseases has undergone many important changes over the past decades, despite the progress of thyroid function immunoassays technologies interferences cannot be completely excluded. These interferences can affect measurement of analyte which leads to misinterpretation and subsequent wrong clinical decisions, the probability of which is about 1%. However, the scale of the problem may be greater due to the lack of awareness to the problem among doctors and the lack of laboratory screening for interfering factors. These factors can be both endogenous and exogenous

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Goncharov, N. P. "Modern methods of hormonal analysis." Problems of Endocrinology 57, no. 1 (2011): 86–91. http://dx.doi.org/10.14341/probl201157186-91.

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Characteristics of the main methods for hormone detection and measurement including radioimmune and immunoenzyme assays are presented along with the detailed description of up-to-date highly sensitive immunoassays of the third generation. These methods are described as allowing determination of virtually the entire range of hormones and biologically active compounds. Their application opens up new opportunities for diagnostics of subclinical forms of endocrine pathology.

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Bohn, Mary Kathryn, Siobhan Wilson, Alexandra Hall, and Khosrow Adeli. "Pediatric reference interval verification for endocrine and fertility hormone assays on the Abbott Alinity system." Clinical Chemistry and Laboratory Medicine (CCLM) 59, no. 10 (2021): 1680–87. http://dx.doi.org/10.1515/cclm-2021-0337.

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Abstract Objectives The Canadian Laboratory Initiative on Pediatric Reference Intervals (CALIPER) has developed an extensive database of reference intervals (RIs) for several biomarkers on various analytical systems. In this study, pediatric RIs were verified for key immunoassays on the Abbott Alinity system based on the analysis of healthy children samples and comparison to comprehensive RIs previously established for Abbott ARCHITECT assays. Methods Analytical performance of Alinity immunoassays was first assessed. Subsequently, 100 serum samples from healthy children recruited with informed

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Sikaris, Ken, Robert I. McLachlan, Rymantas Kazlauskas, David de Kretser, Carol A. Holden, and David J. Handelsman. "Reproductive Hormone Reference Intervals for Healthy Fertile Young Men: Evaluation of Automated Platform Assays." Journal of Clinical Endocrinology & Metabolism 90, no. 11 (2005): 5928–36. http://dx.doi.org/10.1210/jc.2005-0962.

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Abstract Context: Management of male infertility and/or androgen deficiency requires accurate hormonal measurements with valid reference intervals. Objective: The objective of this study was to develop a valid reference panel of blood samples from healthy eugonadal young men with verified normal reproductive function and to use this panel to evaluate the performance of seven fully automated, commercial multiplex immunoassay platforms used to measure serum total testosterone (T), LH, and FSH. Design: This was an observational study of consistency among seven different automated immunoassays for

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Khoo, Serena, Greta Lyons, Anne McGowan, et al. "Familial dysalbuminaemic hyperthyroxinaemia interferes with current free thyroid hormone immunoassay methods." European Journal of Endocrinology 182, no. 6 (2020): 533–38. http://dx.doi.org/10.1530/eje-19-1021.

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Objective Familial dysalbuminaemic hyperthyroxinaemia (FDH), most commonly due to an Arginine to Histidine mutation at residue 218 (R218H) in the albumin gene, causes artefactual elevation of free thyroid hormones in euthyroid individuals. We have evaluated the susceptibility of most current free thyroid hormone immunoassay methods used in the United Kingdom, Europe and Far East to interference by R218H FDH. Methods Different, one- and two-step immunoassay methods were tested, measuring free T4 (FT4) and free T3 (FT3) in 37 individuals with genetically proven R218H FDH. Results With the except

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Narayan, Edward, Frank Molinia, Ketan Christi, Craig Morley, and John Cockrem. "Urinary corticosterone metabolite responses to capture, and annual patterns of urinary corticosterone in wild and captive endangered Fijian ground frogs (Platymantis vitiana)." Australian Journal of Zoology 58, no. 3 (2010): 189. http://dx.doi.org/10.1071/zo10010.

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This study was based on the development of a non-invasive glucocorticoid enzyme-immunoassay for the assessment of stress in wild and captive endangered Fijian ground frogs (Platymantis vitiana). Enzyme-immunoassays were developed and validated for the first time to non-invasively measure both cortisol and corticosterone metabolites in frog urine. Frog urine showed parallel displacement with corticosterone but not cortisol standards, therefore corticosterone enzyme immunoassays were used to examine stress in wild and captive frogs. Urinary corticosterone metabolite concentrations increased in f

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Kumar, Vivek, David R. Barnidge, Li-Sheng Chen, et al. "Quantification of Serum 1–84 Parathyroid Hormone in Patients with Hyperparathyroidism by Immunocapture In Situ Digestion Liquid Chromatography–Tandem Mass Spectrometry." Clinical Chemistry 56, no. 2 (2010): 306–13. http://dx.doi.org/10.1373/clinchem.2009.134643.

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Abstract Background: Immunoassays specific for 1–84 parathyroid hormone (PTH) reportedly reflect the bioactivity of PTH; however, PTH immunoassays can be susceptible to interference by cross-reacting PTH fragments. In addition, these assays currently lack standardization. A methodology using immunocapture purification with liquid chromatography–tandem mass spectrometry (LC-MS/MS) detection, along with a stable isotope–labeled internal standard, may help address these issues. Methods: We isolated 1–84 PTH from 1 mL serum by immunocapture on a 6.5-mm polystyrene bead. The immobilized PTH was dig

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Lepage, N., K. D. Roberts, and J. Langlais. "Interference of lysophosphatidylcholine in hormone radioimmunoassays." Clinical Chemistry 39, no. 5 (1993): 865–69. http://dx.doi.org/10.1093/clinchem/39.5.865.

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Abstract The interference of synthetic and naturally occurring detergents in immunoassays is well documented. In the present study, we evaluated the effect of lysophosphatidylcholine (LPC) and found that the lysophospholipid interfered with formation of the antigen-antibody complex in hormone immunoassays. In the presence of LPC (100 mumol/L), progesterone was overestimated by 29%. Furthermore, physiological concentrations of LPC (140 mumol/L) interfered with the assays of cortisol, progesterone, and aldosterone, resulting in overestimations of 35%, 30%, and 27%, respectively. The addition of

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Soldin, Steven J., and Offie P. Soldin. "Steroid Hormone Analysis by Tandem Mass Spectrometry." Clinical Chemistry 55, no. 6 (2009): 1061–66. http://dx.doi.org/10.1373/clinchem.2007.100008.

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Abstract Background: New high-performance liquid chromatography/tandem mass spectrometry (LC-MS/MS) methods are among the most successful approaches to improve specificity problems inherent in many immunoassays. Content: We emphasize problems with immunoassays for the measurement of steroids and review the emerging role of LC-MS/MS in the measurement of clinically relevant steroids. The latest generation of tandem mass spectrometers has superior limits of quantification, permitting omission of previously employed derivatization steps. The measurement of steroid profiles in the diagnosis and tr

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Öncül, Ümmühan, Fatma Tuba Eminoğlu, Engin Köse, Özlem Doğan, Elif Özsu, and Zehra Aycan. "Serum biotin interference: A troublemaker in hormone immunoassays." Clinical Biochemistry 99 (January 2022): 97–102. http://dx.doi.org/10.1016/j.clinbiochem.2021.10.011.

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Jin, Ming, Mark H. Wener, and Daniel D. Bankson. "Evaluation of automated sex hormone binding globulin immunoassays." Clinical Biochemistry 39, no. 1 (2006): 91–94. http://dx.doi.org/10.1016/j.clinbiochem.2005.10.012.

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Piran, Uri, William J. Riordan, and Deborah R. Silbert. "Effect of hapten heterology on thyroid hormone immunoassays." Journal of Immunological Methods 133, no. 2 (1990): 207–14. http://dx.doi.org/10.1016/0022-1759(90)90361-x.

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Jowett, Terence, Frederick Chu, and Roger Ekins. "Validity of current analog-based free hormone immunoassays." Steroids 52, no. 4 (1988): 365–66. http://dx.doi.org/10.1016/0039-128x(88)90149-3.

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Hamdani Yousra, Hamdani Yousra, Drani Meryem Drani Meryem, El Boukhriss Fatima El Boukhriss Fatima, et al. "The contribution of liquid chromatography coupled to tandem mass spectrometry (LC MS/MS) in the analysis of testosterone." Journal of Medical and Dental Science Research 12, no. 6 (2025): 77–81. https://doi.org/10.35629/076x-12067781.

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The combination of liquid chromatography coupled to tandem mass spectrometry (LC MS/MS) has significantly improved testosterone analysis, offering an analytical method with high sensitivity, specificity and also reproducibility suitable for many applications. This approach is particularly relevant in clinical diagnostics, sports science and the control of substances in dietary supplements. The integration of LC-MS/MS enables accurate and reliable quantification of testosterone concentrations, alleviating the limitations inherent in conventional immunoassays. Testosterone is a key hormone invol

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Karashima, Shigehiro, and Issey Osaka. "Rapidity and Precision of Steroid Hormone Measurement." Journal of Clinical Medicine 11, no. 4 (2022): 956. http://dx.doi.org/10.3390/jcm11040956.

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Steroids are present in all animals and plants, from mammals to prokaryotes. In the medical field, steroids are commonly classified as glucocorticoids, mineralocorticoids, and gonadal steroid hormones. Monitoring of hormones is useful in clinical and research fields for the assessment of physiological changes associated with aging, disease risk, and the diagnostic and therapeutic effects of various diseases. Since the discovery and isolation of steroid hormones, measurement methods for steroid hormones in biological samples have advanced substantially. Although immunoassays (IAs) are widely us

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Marković, Bojan, Ana Petrović-Mucok, Sanja Klet, et al. "Interference in immunoassay measurements of TSH and thyroid hormones after vaccine for Covid-19 or Covid-19 disease." Medicinski glasnik Specijalne bolnice za bolesti štitaste žlezde i bolesti metabolizma 28, no. 91 (2023): 68–78. http://dx.doi.org/10.5937/mgiszm2391068m.

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Immunoassays are commonly used to measure thyroid-stimulating hormone (TSH) and thyroid hormone levels (TH). However, interference in these immunoassays may lead to the misinterpretation of patient results and incorrect therapeutic decisions. Analytical interference can occur following exposure to animals, infections, vaccination, in autoimmune diseases, or without an identified cause. Analytical interference associated with COVID-19 exposure has been observed in D-dimer measurements. A dilution test was used to confirm interference in the measurement of TSH and TH levels. In three patients, i

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Nilsson, C., M. Seppala, and K. Pettersson. "Immunological characterization of human luteinizing hormone with special regard to a common genetic variant." Journal of Endocrinology 168, no. 1 (2001): 107–16. http://dx.doi.org/10.1677/joe.0.1680107.

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Immunoassays are widely used for measuring gonadotropins, including luteinizing hormone (LH), as these are both specific and sensitive. Because LH is microheterogeneous it has been claimed that the specificity of monoclonal antibodies used in two-site immunoassays can limit their clinical utility. Furthermore, we reported earlier a common genetic variant form of LH due to amino acid alterations in the LHbeta gene that is poorly or not recognized by antibodies directed against epitopes present in the intact molecule. We here report the result of an LH epitope mapping using 30 different monoclon

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Piketty, Marie-Liesse, Dominique Prie, Frederic Sedel, et al. "High-dose biotin therapy leading to false biochemical endocrine profiles: validation of a simple method to overcome biotin interference." Clinical Chemistry and Laboratory Medicine (CCLM) 55, no. 6 (2017): 817–25. http://dx.doi.org/10.1515/cclm-2016-1183.

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Abstract Background: High-dose biotin therapy is beneficial in progressive multiple sclerosis (MS) and is expected to be adopted by a large number of patients. Biotin therapy leads to analytical interference in many immunoassays that utilize streptavidin-biotin capture techniques, yielding skewed results that can mimic various endocrine disorders. We aimed at exploring this interference, to be able to remove biotin and avoid misleading results. Methods: We measured free triiodothyronine (fT3), free thyroxine (fT4), thyroid-stimulating hormone (TSH), parathyroid homrone (PTH), 25-hydroxyvitamin

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Sztefko, Krystyna, and Patrycja Szybowska. "Interpretation of Hormone Levels in Older Patients: Points for Consideration." International Journal of Endocrinology 2012 (2012): 1–7. http://dx.doi.org/10.1155/2012/712425.

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Blood hormone and tumor marker concentrations are usually determined by immunochemical methods which are based on an unique reaction between antigen and assay capture antibody. Despite the speed and simplicity of assays performance on automatic immunochemistry platforms, the interpretation of final results requires a deep knowledge of method fallibility. General lack of immunoassays standardization, presence of cross-reacting substances in patient’s sample, limitation of free hormones measurement due to abnormal analyte binding protein concentrations, assay interferences due to patient’s autoa

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Andersson, Anna-Maria, Hans Ørskov, Michael B. Ranke, Steve Shalet, and Niels E. Skakkebæk. "Interpretation of growth hormone provocative tests: comparison of cut-off values in four European laboratories." European Journal of Endocrinology 132, no. 3 (1995): 340–43. http://dx.doi.org/10.1530/eje.0.1320340.

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Andersson A-M, Ørskov H, Ranke MB, Shalet B, Skakkebæk NE. Interpretation of growth hormone provocative tests: comparison of cut-off values in four European laboratories. Eur J Endocrinol 1995;132:340–3. ISSN 0804–4643 To compare interpretations of growth hormone (GH) provocative tests in laboratories using six different GH immunoassays (one enzymeimmunometric assay (EIMA, assay 1), one immunoradiometric assay (IRMA, assay 5), one time-resolved fluorimmunometric assay (TRFIA, assay 3) and three radioimmunoassays (RIAs, assays 2, 4 and 6)), aliquots of peak samples from GH provocative tests wer

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Geno, K. Aaron, Matthew S. Reed, Mark A. Cervinski, and Robert D. Nerenz. "Evaluation of Thyroid Function in Pregnant Women Using Automated Immunoassays." Clinical Chemistry 67, no. 5 (2021): 772–80. http://dx.doi.org/10.1093/clinchem/hvab009.

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Abstract Introduction Automated free thyroxine (FT4) immunoassays are widely available, but professional guidelines discourage their use in pregnant women due to theoretical under-recoveries attributed to increased thyroid hormone binding capacity and instead advocate the use of total T4 (TT4) or free thyroxine index (FTI). The impact of this recommendation on the classification of thyroid status in apparently euthyroid pregnant patients was evaluated. Methods After excluding specimens with thyroid autoantibody concentrations above reference limits, thyroid-stimulating hormone (TSH), FT4, TT4,

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Fisker, S., L. Ebdrup, and H. Ørskov. "Influence of growth hormone binding protein on growth hormone estimation in different immunoassays." Scandinavian Journal of Clinical and Laboratory Investigation 58, no. 5 (1998): 373–82. http://dx.doi.org/10.1080/00365519850186355.

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Boggs, Ashley S. P., Thomas M. Galligan, Jennifer V. Kemp, Singh J. Ravinder, and Stefan Karl Gunther Grebe. "The Pitfalls of Using Research Grade Immunoassays for Thyroid Health Measurements." Journal of the Endocrine Society 5, Supplement_1 (2021): A834. http://dx.doi.org/10.1210/jendso/bvab048.1701.

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Abstract Introduction: Immunoassay technology is subject to matrix interferences that can produce inaccurate results and incorrect conclusions when using samples not previously validated. While many commercially available research grade (RG) immunoassay kits are available, caution should be applied when using RG kits for thyroid health assessments, particularly on samples from pregnant individuals whose blood chemistry is unique to non-pregnant individuals. Question: Do RG immunoassay kits reliably provide precise and accurate measurements of thyroid health biomarkers in serum Standard Referen

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Turner, Katherine A., Bethany J. Larson, Nina C. Kreofsky, Maria Alice V. Willrich, Joshua A. Bornhorst, and Alicia Algeciras-Schimnich. "Assessment of complement interference in anti-Müllerian hormone immunoassays." Clinical Chemistry and Laboratory Medicine (CCLM) 58, no. 1 (2019): e8-e10. http://dx.doi.org/10.1515/cclm-2019-0496.

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Owen, William E., Mary Lou Gantzer, Jeremy M. Lyons, Alan L. Rockwood, and William L. Roberts. "Functional sensitivity of seven automated thyroid stimulating hormone immunoassays." Clinica Chimica Acta 412, no. 23-24 (2011): 2336–39. http://dx.doi.org/10.1016/j.cca.2011.08.002.

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Su, H. Irene, Mary D. Sammel, Michael V. Homer, Kim Bui, Carolyn Haunschild, and Frank Z. Stanczyk. "Comparability of antimüllerian hormone levels among commercially available immunoassays." Fertility and Sterility 101, no. 6 (2014): 1766–72. http://dx.doi.org/10.1016/j.fertnstert.2014.02.046.

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Piketty, Marie-Liesse, Michel Polak, Isabelle Flechtner, Laura Gonzales-Briceño, and Jean-Claude Souberbielle. "False biochemical diagnosis of hyperthyroidism in streptavidin-biotin-based immunoassays: the problem of biotin intake and related interferences." Clinical Chemistry and Laboratory Medicine (CCLM) 55, no. 6 (2017): 780–88. http://dx.doi.org/10.1515/cclm-2016-0606.

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Abstract Immunoassays are now commonly used for hormone measurement, in high throughput analytical platforms. Immunoassays are generally robust to interference. However, endogenous analytical error may occur in some patients; this may be encountered in biotin supplementation or in the presence of anti-streptavidin antibody, in immunoassays involving streptavidin-biotin interaction. In these cases, the interference may induce both false positive and false negative results, and simulate a seemingly coherent hormonal profile. It is to be feared that this type of errors will be more frequently obs

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Lopez, Mary F., Taha Rezai, David A. Sarracino, et al. "Selected Reaction Monitoring–Mass Spectrometric Immunoassay Responsive to Parathyroid Hormone and Related Variants." Clinical Chemistry 56, no. 2 (2010): 281–90. http://dx.doi.org/10.1373/clinchem.2009.137323.

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Abstract Background: Parathyroid hormone (PTH) assays able to distinguish between full-length PTH (PTH1–84) and N-terminally truncated PTH (PTH7–84) are of increasing significance in the accurate diagnosis of endocrine and osteological diseases. We describe the discovery of new N-terminal and C-terminal PTH variants and the development of selected reaction monitoring (SRM)-based immunoassays specifically designed for the detection of full-length PTH [amino acid (aa)1–84] and 2 N-terminal variants, aa7–84 and aa34–84. Methods: Preparation of mass spectrometric immunoassay pipettor tips and MALD

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Geiseler, D., P. Chodha, and R. Ekins. "One-step immunoassays for free (unbound) hormones: the effect of tracer binding by serum proteins." Clinical Chemistry 32, no. 1 (1986): 45–49. http://dx.doi.org/10.1093/clinchem/32.1.45.

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Abstract In binding assays for determination of free (non-protein-bound) hormones in serum or plasma, the influence of the measuring system on the original analyte concentration in the sample must be considered. In one- or single-step free-hormone immunoassays, the labeled analyte or analog-tracer not only is bound to the antibody, it also is bound, to some extent, to serum proteins. The dependence of the assay response on two unknown variables--the concentration of free analyte and the binding potential of serum for the tracer--introduces a bias between the actual (original) and measured horm

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Kim, John J., Wenchuan Liang, Chi-Chih Kang, Mark D. Pegram та Amy E. Herr. "Single-cell immunoblotting resolves estrogen receptor-α isoforms in breast cancer". PLOS ONE 16, № 7 (2021): e0254783. http://dx.doi.org/10.1371/journal.pone.0254783.

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An array of isoforms of the nuclear estrogen receptor alpha (ER-α) protein contribute to heterogeneous response in breast cancer (BCa); yet, a single-cell analysis tool that distinguishes the full-length ER-α66 protein from the activation function-1 deficient ER-α46 isoform has not been reported. Specific detection of protein isoforms is a gap in single-cell analysis tools, as the de facto standard immunoassay requires isoform-specific antibody probes. Consequently, to scrutinize hormone response heterogeneity among BCa tumor cells, we develop a precision tool to specifically measure ER-α66, E

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Yesildal, Fatih, Muhittin Serdar, and Taner Ozgurtas. "A practical ID-LC-MS/MS method for the most commonly analyzed steroid hormones in clinical laboratories." Turkish Journal of Biochemistry 44, no. 2 (2018): 130–41. http://dx.doi.org/10.1515/tjb-2018-0214.

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Abstract Background Analysis of steroid hormones rapidly and reliably remains a challenge in clinical laboratories as this plays an important role in evaluation of many endocrine disorders. The aim of this study was to create a steroid profiling panel by using a liquid chromatography tandem mass spectrometry (LC-MS/MS) method which was composed of the most commonly analyzed steroid hormones in clinical laboratories. Materials and methods Protein precipitation was performed for sample preparation. Ultra performance liquid chromatography (UPLC) system and an analytical column with C18 selectivit

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Leung, Edward K. Y., Christine C. Lee, Peter Angelos, et al. "Analytical Differences in Intraoperative Parathyroid Hormone Assays." Journal of Applied Laboratory Medicine 3, no. 5 (2019): 788–98. http://dx.doi.org/10.1373/jalm.2018.026815.

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Abstract Background We compared the rates of intraoperative parathyroid hormone (PTH) decline using the Siemens Immulite® Turbo PTH and Roche Elecsys® short turnaround time PTH assays in 95 consecutive surgical patients to investigate analytical and turnaround time (TAT) differences between the tests performed in the operating room (OR) vs the central clinical chemistry laboratory (CCL). Methods Serial blood samples from 95 patients undergoing parathyroidectomy were collected and measured using the 2 immunoassays. Specimens from the first 15 patients were measured simultaneously in the OR and

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Arroofita Ani Sandiya, Sudjarwo та Ashon Sa’adi. "MEMBANDINGKAN REGRESI 4PL DAN LINIER FIT UNTUK VERIFIKASI HORMON 17β-ESTRADIOL MENGGUNAKAN METODE ELISA". Journal of Research and Technology 5, № 1 (2019): 42–49. http://dx.doi.org/10.55732/jrt.v5i1.445.

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One solution for infertile couples to get offspring is IVF, one of the stages is a HOT procedure. On the stage there is an increasing in steroid hormone levels (estrogen) as a result of ovarian follicles development. The 17β-estradiol hormone was chosen to be verified because it can be used as a marker or marker to show the maturity follicle. Linear and logistic regression are the two most commonly used in curve making models for ELISA sandwich immunoassays. Although linear regression may be useful when analyzing samples included in the linear part of the analyte response curve, logistic regre

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