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1
Rockwood, Jananie. "House Dust Mite Induced Gene Expression and Cytokine Secretion by Human Dermal Fibroblasts." Wright State University / OhioLINK, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=wright1347976529.
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Newman, Aaron Mathew. "The Response of Vascular Dermal Enodethial Cells to House Dust Mite Extracts." Wright State University / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=wright1205717763.
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Hay, David B. "Ecology of the house dust mite Dermatophagoides pteronyssinus (Trouessart)." Thesis, University of Oxford, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.302975.
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4
Walshaw, M. J. "Allergen avoidance in house dust mite sensitive adult asthma." Thesis, University of Liverpool, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.354527.
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Causton, Benjamin. "Mechanisms underlying the immune response to inhaled house dust mite." Thesis, Imperial College London, 2012. http://hdl.handle.net/10044/1/9160.
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Asthma is a chronic inflammatory disease of the airways and is characterised by airway hyperresponsiveness (AHR), inflammation and remodelling. House dust mite (HDM; Dermatophagoides pteronyssinus) is a complex aeroallergen, commonly associated with development of allergic asthma. Animal models have been utilised extensively to model the traits of asthma and HDM-induced allergic airways disease was established in mice following serial HDM challenge via the respiratory mucosa. Mice exposed to HDM developed pulmonary eosinophilia, characterised by Th2 cytokine production, concomitant with AHR and airway remodelling. Following the establishment and characterisation of this model of allergic airways disease, it was next investigated which features of the HDM allergen were responsible for disease pathogenesis. Using genetically modified mice and pharmacological approaches, it was found that the TLR-TRIF signalling pathway played a crucial role in HDM-induced allergic airways disease. Intrinsic protease activity of the HDM extract was also observed to be vital for disease pathogenesis, whereby mice exposed to boiled HDM developed a less severe asthma phenotype. Utilising a pan neutralising antibody directed towards transforming growth factor-β (TGF-β), TGF-β was shown to play a critical role in regulating HDM-induced airway inflammation in vivo. Following therapeutic blockade of TGF-β, the numbers of CD4+CD25+FoxP3+ regulatory T cells and CD4+IL-10+ cells were decreased resulting in exacerbation of AHR and BAL inflammation compared to HDM-treated isotype control mice. However, HDM-induced airway remodelling progressed independently of TGF-β. In conclusion, it has been determined that HDM-induced Th2-driven inflammation, AHR and airway remodelling in mice is induced by multiple features of the allergen and that TGF-β regulates HDM-driven airway inflammation. Thus these findings provide an insight into the mechanisms by which the aeroallergen HDM promotes allergic disease and will aid in the development of potential new therapeutic strategies for the treatment of asthma.
6
Young, R. P. "House dust mite sensitization : the role of genetic and environmental factors." Thesis, University of Oxford, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.334956.
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Roberts, Holly Ann. "Equine Intradermal Test Threshold Concentrations for House Dust Mite and Storage Mite Allergens and Identification of Stable Fauna." The Ohio State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=osu1396694230.
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Martinez, Giancarlo Lopez. "Environmental and Behavioral control of the American House Dust Mite, Dermatophagoides Farinae Hughes." The Ohio State University, 2003. http://rave.ohiolink.edu/etdc/view?acc_num=osu1392822781.
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9
Sharif, Sameena. "Biodegradable microparticles as delivery systems for the allergens of Dermatophagoides pteronyssinus (house dust mite)." Thesis, University of Nottingham, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.294244.
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10
Chen, Hao Hang Rachel. "The effect of pandemic influenza H1N1 viral infection on house dust mite sensitized mice." Thesis, University of British Columbia, 2016. http://hdl.handle.net/2429/58438.
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A disproportionately large number of asthmatics experienced morbidity and mortality during the 2009 H1N1 pandemic. To date, there is little information on the mechanisms behind this epidemiological and clinical observation. Using a murine asthma model, we sought to determine the effects of airway inflammation on host responses to pandemic H1N1 (pH1N1) infection. We hypothesized that mice with an allergic airway phenotype would have a greater viral susceptibility to pH1N1 infection and a dysregulated host response that prevents effective viral clearance and leads to increased burden of pulmonary inflammation, resulting in poor clinical outcomes. We established a murine allergic airway model using house dust mite (HDM) extract. We intranasally instilled male BALB/c mice with HDM or sham PBS daily for two weeks; after which we introduced a single intranasal dose of pH1N1 virus or control vehicle fluid (CAF). HDM or PBS instillation continued daily post-viral infection (pi) forming four groups: 1) sham-sensitized + CAF, 2) HDM-sensitized + CAF, 3) sham-sensitized + pH1N1, and 4) HDM-sensitized + pH1N1. Mice were weighed daily. Virus-infected animals were euthanized at 1-hr pi and on Day 1, 2, 4, 5, 6, and 8 pi and non-infected animals were euthanized on Day 0 and 8 pi. Viral titre, interferon-β (IFN β), and interferon-stimulated gene (ISG) expression patterns were determined by qPCR on RNA extracted from homogenized lung tissue. IFN β protein levels were evaluated by ELISA in bronchoalveolar lavage. Pulmonary inflammation was quantified using H&E stain on formalin-fixed paraffin-embedded lung tissue. HDM-sensitized animals exhibited significantly greater weight loss than sham-sensitized animals following infection. Also, HDM-sensitized mice had significantly higher viral titres on Day 8 pi as compared to sham-sensitized mice. Downstream ISG inductions were dampened in HDM-sensitized, virus-infected animals despite comparable initial IFN β response in HDM- and sham-sensitized mice. We also observed mixed-type pulmonary inflammation in HDM-sensitized mice following pH1N1 infection. Our data suggest dysregulated host ISG responses, combined with the overwhelming burden of pulmonary inflammation, contribute to impaired viral clearance and weight loss indicative of detrimental health outcomes in animals sensitized with HDM following pH1N1 infection.<br>Medicine, Faculty of<br>Experimental Medicine, Division of<br>Medicine, Department of<br>Graduate
11
Furmonaviciene, Ruta. "Structural studies of Der p 1, the major house dust mite allergen, and its homologues." Thesis, University of Nottingham, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.342488.
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Tabbah, Khaldoun. "Specific immunotherapy for perennial allergic rhinitis." Thesis, University of Southampton, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.299414.
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Dunn, Graham Spencer. "Crystallographic and biochemical analysis of three distinct hydrolases : dermatophagoides pteronyssinus 1(Der p1), momordin and the bacterial carbon-carbon hydrolase, MhpC." Thesis, University of Southampton, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.340364.
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14
Rusznak, Csaba. "The effects of cigarette smoke and house dust mite allergens on human bronchial epithelial cell function." Thesis, Queen Mary, University of London, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.313302.
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Joseph, Karen Elizabeth. "The effect of providing bedding encasings on adherence to dust mite control procedures in pediatric asthma patients." Morgantown, W. Va. : [West Virginia University Libraries], 2000. http://etd.wvu.edu/templates/showETD.cfm?recnum=1605.
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Thesis (M.A.)--West Virginia University, 2000.<br>Title from document title page. Document formatted into pages; contains viii, 123 p. Includes abstract. Includes bibliographical references (p. 63-68).
16
Lam, Diane Phuong Nghinh. "Characterization of the immunomodulatory activities of sterile house dust extracts in a murine model of asthma." Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2008. http://wwwlib.umi.com/cr/ucsd/fullcit?p1447798.
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Thesis (M.S.)--University of California, San Diego, 2008.<br>Title from first page of PDF file (viewed Feb. 5, 2008). Available via ProQuest Digital Dissertations. Includes bibliographical references (p. 60-66).
17
Leitch, David Neil. "An investigation into the effects of annual residential change on asthmatic symptoms in university students." Thesis, University of Newcastle Upon Tyne, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.369833.
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Wright, Gillian R. "The effect of domestic mechanical heat recovery ventilation on asthma control of patients allergic to the house dust mite." Thesis, University of Glasgow, 2008. http://theses.gla.ac.uk/189/.
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The prevalence of asthma has increased over the last generation, in parallel with a warm indoor microclimate. Central heating, fitted carpets and tight building construction have improved standards of heating and energy efficiency in homes, at the expense of ventilation. A warm, humid environment favours the growth of the house dust mite population. Allergy to the house dust mite is the most common allergy associated with asthma in the UK. Studies of occupational asthma, seasonal asthma and at altitude infer that the environment may directly affect symptoms of asthma. Allergen avoidance has been advocated as an important aspect of asthma management, yet the evidence for its efficacy has not been clear. Large studies of conventional measures to eradicate dust mites, such as mattress covers, have not shown a benefit for symptoms of asthma. As house dust mites are sensitive to humidity, an additional strategy would be to reduce indoor air humidity by improving ventilation. A randomised, double-blind placebo-controlled study examined the effect of the installation of domestic mechanical heat recovery ventilation on asthma control in the homes of 119 adults sensitive to house dust mite allergen. The study involved collaboration between the University Departments of Architecture, Respiratory Medicine and Immunology, local General Practices, the district general hospital, the local councils and industry. 100 participants completed follow-up. At twelve months, there was a clinically significant improvement in evening peak expiratory flow in the mechanical ventilation group and fewer admissions to hospital with asthma. There was a non-significant improvement in the mechanical ventilation group in the primary outcome, morning peak expiratory flow. There was a significant reduction in the asthma control questionnaire score at 3 months, but this was not sustained to 12 months. Rhinitis visual analogue scores for sneezing, nasal discharge and nasal blockage significantly improved in the group with mechanical ventilation compared to the control group at 6 months, but not at 12 months. There was no difference in exhaled nitric oxide, a measure of airway inflammation, between the two groups at 12 months. However, these clinical improvements could not be explained by reduced allergen exposure, as although indoor air humidity was reduced during the winter months, there was no difference between the house dust mite levels between the groups, nor in the levels of specific IgE to the house dust mite. Other mechanisms, such as mould, endotoxin, viral infection and environmental tobacco smoke should be considered in future work. In the mechanical ventilation group there was a modest individual gain of 0.02 Quality-adjusted life years over 12 months. However, it may still prove a cost-effective intervention if the clinical effects are sustained. Further research is required to establish if the clinical effects are sustained for greater than one year and to investigate the mechanism of the effect of improved home ventilation on respiratory health.
19
Colloff, M. J. "The biology and control of the European House Dust Mite, Dermatophagoides pteronyssinus (Trouessart, 1897) (Acari:Pyroglyphidae) in relation to atopic allergy." Thesis, University of Glasgow, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.375455.
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Mushaben, Elizabeth M. "BMPR2 and mTOR Signaling Pathways in Inflammatory Lung Diseases." University of Cincinnati / OhioLINK, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1352485302.
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Taylor, Rebecca Chantelle. "Effects of toll-like receptor 2 ligands on T-cell responses to mite allergen in humans." University of Western Australia. School of Paediatrics and Child Health, 2007. http://theses.library.uwa.edu.au/adt-WU2007.0107.
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[Truncated abstract] The last few decades have witnessed an increase in the prevalence, morbidity and economic burden associated with asthma and allergic disease. This rising incidence cannot be completely explained by changes in genetic factors or by improvements in diagnostic procedures. Environmental factors, particularly those associated with a westernised lifestyle, are considered to be involved in this increase. In the late 1980’s Strachan was the first to link environmental factors with allergic disease, this theory became to be known as the ‘hygiene hypothesis’. This hypothesis links the “cleaner” more “healthy ” environment we now live in, with an increased risk of developing allergic disease. This effect is highlighted by studies linking farm and animal exposure (rich in microbial compounds) during early life with a decrease in allergic disease. Since then numerous studies have been undertaken to ascertain the factors present in the microbe rich environment, which elicit this protective effect. Many studies have revolved around endotoxin, however microbial components (mainly from Gram-positive bacteria) which signal through Toll-like receptor 2 (TLR2), have also shown that they can alter the allergic immune response. In mice models TLR2 has been shown to both exacerbate and inhibit allergic disease. The above research highlights the need for further studies into the effect of TLR2 ligands, and to define the mechanisms by which they exert their effects in human allergic disease. These mechanisms will be relevant to understanding the pathogenesis of allergy, but also might provide novel ways to treat allergy. The aims of the study outlined in this thesis were to determine whether in vitro exposure to TLR2 ligands could modify the established immune response to house dust mite allergen (HDM), and to examine the mechanisms by which this occurs. ... The addition of glucocorticoids to LTA enhanced the ability of this TLR2 ligand to inhibit IL-5 and IL-13 production by HDM-activated blood mononuclear cells. In conclusion, this study shows that TLR2 ligands have the ability to inhibit the Th2 response to mite allergen in previously sensitized individuals by an as yet unknown mechanism. However the findings described herein do provide an impetus for future studies designed to uncover novel mechanisms by which allergic responses can be ameliorated, and may open new treatment modalities.
22
Zhao, Min [Verfasser], and Harald [Akademischer Betreuer] Renz. "Development of a house dust mite model of mixed allergic airway inflammation and analysis of allergyprotective effects of Staphylococcus sciuri / Min Zhao. Betreuer: Harald Renz." Marburg : Philipps-Universität Marburg, 2012. http://d-nb.info/1027183980/34.
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Madouri, Fahima. "Asthme allergique induit par un allergène d’acarien, House Dust Mite (HDM) : rôles de la caspase-1 et de la protéine kinase C thêta (PKC-θ)". Thesis, Orléans, 2014. http://www.theses.fr/2014ORLE2055/document.
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Des études menées au laboratoire avaient démontré un rôle critique de l’inflammasome NLRP3 dans l’asthme allergique en réponse à l’ovalbumine en absence d’adjuvant. Mes travaux de thèse ont porté sur le rôle de NLRP3 et de la caspase-1 dans un modèle murin d’inflammation pulmonaire induite par l’allergène d’acarien HDM. Nous avons montré un rôle régulateur de la caspase-1 dépendant de l’inflammasome NLRP3 et la molécule adaptatrice ASC mais pas de l’inflammasome NLRC4. Cette régulation de la réponse allergique se caractérise par une augmentation de l’infiltration des éosinophiles, de l’hyperréactivité bronchique et de la production des cytokines de type Th2 telles que l’IL-4, l’IL-5, l’IL-13 et l’IL-33 dans les poumons. Nous avons montré que les mécanismes responsables de cette régulation sont associés à l’IL-33 produite par les macrophages et que la neutralisation de l’IL-33 par administration locale de la protéine de fusion au récepteur ST2 (muST2-Fc) atténue les caractéristiques de l’asthme allergique. Ces résultats suggèrent que l’activation de la caspase-1 réduit la production d’IL-33 in vivo et régule ainsi la réponse l’inflammation pulmonaire induite par HDM et la réponse Th2. D’autre part, nous nous sommes intéressés au rôle de la Protéine Kinase C thêta (PKC-θ) dans ce même modèle d’inflammation pulmonaire. Nous avons démontré que PKC-θ joue non seulement un rôle protecteur dans l’asthme allergique mais également un rôle critique pour la prolifération et l’activation des cellules lymphoïdes innées (ILC2). D’autre part, l’inhibition de PKC-θ in vivo par administration orale de son inhibiteur spécifique C20 (BIX02656) atténue l’inflammation pulmonaire et la production d’IL-5 et d’IL-13. Nous suggérons que PKC-θ est impliquée dans la différenciation des Th2 et des ILC2 via un mécanisme dépendant des facteurs de transcription IRF4 et NFAT-1. Au total, mes travaux de thèse mettent en exergue deux molécules IL-33 et PKC-θ qui pourraient constituer des cibles thérapeutiques potentielles<br>Studies from our laboratory have shown a critical role of NLRP3 inflammasome in response to ovalbumin allergen. In the present study we investigate the role of NLRP3 and caspase-1 in a mouse model of pulmonary inflammation induced by HDM. We have shown a regulatory role of caspase-1 dependant of the NLRP3 inflammasome and the adaptator molecule ASC but not NLRC4. The regulation of the allergic response is characterized by an increase of eosinophilia, bronchial hyperreactivity and Th2 cytokines production (IL-4, IL-5, IL-13 and IL-33) in lungs. We have shown that mechanisms responsible of this regulation are associated with IL-33 production by macrophages and that neutralization of IL-33 by local administration of a fusion protein of the ST2 receptor (muST2-Fc) reduce characteristics of asthma. These results suggest that caspase-1 activation reduce IL-33 production in vivo regulating lung inflammation and Th2 response induced by HDM. Moreover, we investigate the role of the Protein Kinase C theta (PKC-θ) in allergic airway inflammation. We have demonstrated that PKC-θ plays a protective role in allergic asthma but is critical for the activation and proliferation of innate lymphoid cells (ILC2). In addition, in vivo inhibition by oral administration of PKC-θ specific inhibitor C20 (BIX02656) reduces pulmonary inflammation with IL-5 and IL-13 production. We suggest that PKC-θ is implicated in Th2 and ILC2 differenciation by a mechanism dependant on transcription factors IRF4 and NFAT-1. Finally, my thesis projects describe IL-33 and PKC-θ as potential therapeutic targets for allergic lung inflammation
24
Chambers, Louise Jane. "Enzymes as allergens : the enzymatic characterisation and recombinant expression of the house dust mite allergen Der p 1, and the immune response to enzymatically active papain." Thesis, University of Nottingham, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.342047.
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Kramer, Elizabeth L. "Role of the EGFR Pathway in Lung Remodeling and Disease." University of Cincinnati / OhioLINK, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1250206890.
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Zinkevičienė, Auksė. "Yeast in atopic dermatitis etiology." Doctoral thesis, Lithuanian Academic Libraries Network (LABT), 2012. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2012~D_20121107_091213-63157.
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Isolation and identification of all yeast species found on skin affected by atopic dermatitis, evaluation of their influence to the synthesis of IgE antibodies, and assessment of the possible cross-reactivity between different yeast species was performed. It was shown that in 36.9 % of the cases of atopic dermatitis, the affected skin was colonized with yeast belonging to three genera: Candida, Malassezia and Rhodotorula. Systematic and phylogenetic analysis of sequences from atypical Malassezia restricta strain M8 indicated that this isolate could be a member of a new yeast species. Three atypical Malassezia isolates M47, M54 and M235 were identified as non-lipid-dependent variants of Malassezia furfur. It was shown that in atopic dermatitis, cutaneous colonization with yeast is two-fold higher in adults than in children. The sera of atopic dermatitis patients have specific IgE antibodies to cross-reactive intracellular yeast antigens. Candida pelliculosa and house dust mites Dermatophagoides pteronyssinus and Dermatophagoides farinae might share some allergenic epitopes. The results of this study suggest that attention should be given to a cutaneous colonization by saprophytic yeast since the immune response to the allergens could further exacerbate allergic inflammation due to cross-reactive epitopes.<br>Išskirtos ir identifikuotos atopinio dermatito pažeistą odą kolonizuojančios mielių rūšys, įvertinta jų įtaka specifinių IgE antikūnų sintezei bei kryžminių reakcijų tarp skirtingų mielių rūšių galimybė. Nustatyta, kad 36,9 % atvejų atopinio dermatito pažeista oda yra kolonizuojama Candida, Malassezia ir Rhodotorula genties mielėmis. Išskirtas netipinėmis fiziologinėmis savybėmis pasižymintis Malassezia restricta kamienas M8 gali būti naujos rūšies atstovas. Išskirti netipinėmis fiziologinėmis savybėmis pasižymintys Malassezia genties kamienai M47, M54 ir M235 identifikuoti kaip nuo išorinio lipidų šaltinio nepriklausantys Malassezia furfur. Įrodyta, kad mielės suaugusių asmenų atopinio dermatito pažeistą odą kolonizuoja du kartus dažniau negu vaikų. Įrodyta, kad atopiniu dermatitu sergančių asmenų kraujo serume aptinkama prieš kryžmiškai reaguojančius mielių viduląstelinius antigenus nukreiptų specifinių IgE antikūnų. Taip pat nustatyta, kad Candida pelliculosa ir namų dulkių erkių Dermatophagoides pteronyssinus ir Dermatophagoides farinae alergenai gali turėti panašius epitopus. Darbo rezultatai patikimai rodo, kad atopinio dermatito pažeistą odą kolonizuojančios komensalinės mielės gali pasunkinti atopinio dermatito eigą dėl kryžmiškai reaguojančių epitopų tarp skirtingų biologinių rūšių antigenų.
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Siman, Isabella Lima. "Atividade bloqueadora de anticorpos IgG específicos purificados de soros de pacientes atópicos a ácaros sobre a reatividade de IgE a Dermatophagoides pteronyssinus por ELISA inibição." Universidade Federal de Uberlândia, 2013. https://repositorio.ufu.br/handle/123456789/12772.
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One of the purposes of allergen-specific immunotherapy (SIT) is to modulate the humoral
immune response against allergens with significant increases in allergen-specific IgG1 and
IgG4 levels. These antibodies are associated with blocking activity by preventing IgE binding
to allergen and leading to reduced inflammatory responses. This study aimed to investigate in
vitro blocking activity of allergen-specific IgG antibodies on IgE reactivity to D.
pteronyssinus (Dpt) in sera from atopic patients. Dpt-specific IgG antibodies were obtained
from atopic sera and irrelevant IgG from non-atopic sera. IgG antibodies were purified by
ammonium sulfate precipitation followed by Protein-G affinity chromatography and evaluated
with regards to purity by SDS-PAGE and immunoreactivity by slot-blot and immunoblot
assays. The blocking activity was evaluated by inhibition ELISA. The electrophoretical
profile after salting-out precipitation showed an enrichment of high molecular weight proteins
in the precipitated fraction and strongly stained bands in the ligand fraction after
chromatography, compatible with molecular weight of human IgG. It was detected strong
immunoreactivity to IgG, negligible to IgA, and no reactivity to IgE and IgM. Dpt-specific
IgG fraction was capable to significantly reduce levels of IgE anti-Dpt, resulting in 35-51%
inhibition of IgE reactivity to Dpt in atopic patient sera. Allergen-specific IgG antibodies
purified using available and standardized methodology are able to inhibit IgE reactivity to Dpt
allergen extract. In addition to the clinical symptoms improvement (subjective parameter),
this approach reinforces that the intermittent measurement of serum allergen-specific IgG
antibodies will be an important objective laboratorial parameter that will help specialists to
follow their patients under SIT.<br>Uma das propostas da imunoterapia alérgeno específica é a de modular a resposta imune
humoral contra alérgenos, com aumento significativo nos níveis de IgG1 e IgG4 específicos.
Esses anticorpos estão associados com uma atividade bloqueadora, impedindo a ligação de
anticorpos IgE ao alérgeno e levando a uma redução nas respostas inflamatórias. Esse estudo
objetivou investigar a atividade bloqueadora, in vitro, de anticorpos IgG específicos sobre a
reatividade de IgE a D. pteronyssinus (Dpt) em soros de pacientes atópicos. Anticorpos IgG
específicos foram obtidos de soros de pacientes atópicos, e IgG irrelevante a partir de soros de
não atópicos, e depois purificados por precipitação com sulfato de amônio, seguido de
cromatografia de afinidade em Proteina G-agarose. A pureza desses anticorpos foi avaliada
por SDS-PAGE, a imunoreatividade por ensaios de slot-blot e immunoblot, e a atividade
bloqueadora por ELISA inibição. O perfil eletroforético, após precipitação com sulfato de
amônio, mostrou um enriquecimento de proteínas de alto peso molecular na fração
precipitada,e bandas fortemente coradas na fração ligante após a cromatografia, compatíveis
com o peso molecular de IgG humana. Foi detectada uma forte imunoreatividade para IgG,
leve para IgA, e nenhuma reatividade para IgE e IgM. A Fração IgG específica foi capaz de
reduzir significantemente os níveis de IgE anti-Dpt, resultando em 35-51% de inibição da
reatividade de IgE a Dpt em pools de soros de pacientes atópicos. Anticorpos IgG específicos
purificados, através de uma metodologia disponível e padronizada, são capazes de inibir a
reatividade de IgE ao extrato alergênico Dpt. Além da melhoria da sintomatologia clínica,
considerada um parâmetro subjetivo, essa abordagem reforça que a avaliação intermitente de
anticorpos IgG alérgeno-específicos pode ser uma ferramenta importante, auxiliando
especialistas a acompanharem seus pacientes em processo de imunoterapia específica.<br>Mestre em Ciências da Saúde
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Nu?ez, Nail? Karine. "Efeitos da defici?ncia de vitamina D na fun??o pulmonar de um modelo de asma al?rgica experimental." Pontif?cia Universidade Cat?lica do Rio Grande do Sul, 2017. http://tede2.pucrs.br/tede2/handle/tede/7857.
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Previous issue date: 2017-07-12<br>Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES<br>Background: Asthma is a chronic disease of the airways, characterized by bronchial inflammation and hyperresponsiveness, which affects approximately 300 million people around the world. The increase in the prevalence of asthma in recent years has been associated with an increase in vitamin D deficiency. About 1 billion people in the world have insufficient levels of vitamin D due to many factors such as reduced outdoor activities, sunscreens use and a diet low in vitamin D. In addition, many studies suggest that vitamin D deficiency has a direct effect on lung function, leading to changes in the structure of the airways and in the inflammatory process.
Objectives: To evaluate the effect of vitamin D deficiency at different life stages in a murine model of allergic airways disease house dust mite (HDM) induced on inflammation and lung function.
Methods: Female BALB / c mice were placed in a diet replete or deficient in vitamin D at three-week old. At 8 weeks, females were mated with males on a diet replete in vitamin D. At birth, pups were cross-fostered to assess the effects of vitamin D deficiency at different stages of life, in utero (Vit D -/+), postnatal (Vit D +/-) and whole-life (Vit D - / -) compared to the control group whole-life vitamin D replete (Vit D + / +). At 8 weeks of age, mice of both sexes were challenged for 10 consecutive days intranasally with either HDM extract or saline solution after mild anesthesia. The animals were anesthetized for lung function test and then submitted to euthanasia for bronchoalveolar lavage (BAL) and lung tissue removal 24 hours after the last intranasal challenge. The total BAL cell count and collagen quantification of lung tissue homogenized were evaluated.
Results: Vitamin D deficiency did not affect HDM-induced inflammation, which was characterized by BAL eosinophilia. Vitamin D deficiency at any life stage (in utero, postnatal and all life) caused impairment of lung function, increased tissue damping and tissue elastance, being particularly observed in females. On the other hand, the asthma HDM-induced decreased airway distensibility, but only in females and vitamin D do not altered this response.
Conclusion: Our results suggest that vitamin D and HDM have different mechanisms that influence in the development of allergic lung disease and furthermore the effects appear to be sex-specific.<br>Introdu??o: A asma ? uma doen?a cr?nica das vias a?reas, caracterizada por inflama??o e hiperresponsividade br?nquica, que atinge aproximadamente 300 milh?es de pessoas ao redor do mundo. O aumento da preval?ncia da asma nos ?ltimos anos tem sido associado ao aumento da defici?ncia de vitamina D. Cerca de 1 bilh?o de pessoas no mundo apresenta n?veis insuficientes de vitamina D em fun??o de diversos fatores, tais como a redu??o de atividades ao ar livre, uso de protetor solar e dieta pobre em vitamina D. Al?m disso, estudos sugerem que a defici?ncia de vitamina D possui um efeito direto na fun??o pulmonar, causando altera??es na estrutura das vias a?reas e no processo inflamat?rio.
Objetivo: Avaliar o efeito da defici?ncia de vitamina D em diferentes est?gios da vida de camundongos com asma induzida por ?caro domiciliar (house dust mite; HDM) sobre a inflama??o e fun??o pulmonar.
M?todos: Camundongos BALB/c f?meas receberam dieta rica ou deficiente em vitamina D a partir da terceira semana de vida. Com 8 semanas de vida, as f?meas foram acasaladas com machos em dieta rica em vitamina D. Ao nascimento foi realizado o cross-fostering (ado??o cruzada) com a prole para que fosse poss?vel avaliar o efeito da defici?ncia de vitamina D em diferentes est?gios da vida, in utero (Vit D -/+), p?s-natal (Vit D +/-) e durante toda a vida (Vit D -/-), em compara??o ao grupo controle, que recebeu dieta rica em vitamina D durante toda a vida (Vit D +/+). Com 8 semanas de vida, camundongos de ambos os sexos foram desafiados por 10 dias consecutivos por via intranasal, com extrato de HDM ou apenas solu??o salina. Os animais foram anestesiados para a realiza??o do teste de fun??o pulmonar e ent?o submetidos a eutan?sia para a realiza??o do lavado broncoalveolar (LBA) e retirada do tecido pulmonar, 24 horas ap?s o ?ltimo desafio intranasal. Foi avaliada a contagem total de c?lulas do LBA e quantifica??o de col?geno no homogeneizado de tecido pulmonar.
Resultados: A defici?ncia de vitamina D n?o afetou a inflama??o induzida por HDM, que foi caracterizada por eosinofilia no LBA. A defici?ncia de vitamina D em qualquer fase da vida dos camundongos (in utero, p?s-natal e durante toda a vida) causou uma piora na fun??o pulmonar, aumentando o tissue damping e tissue elastance, sendo observado particularmente em f?meas. Por outro lado, a asma induzida por HDM diminuiu a distensibilidade das vias a?reas apenas em f?meas e a vitamina D n?o alterou essa resposta.
Conclus?o: Nossos resultados sugerem que a vitamina D e HDM possuem diferentes mecanismos que influenciam no desenvolvimento da doen?a pulmonar al?rgica e, al?m disso, os efeitos parecem ser dependentes do sexo.
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Rigaux, Peter. "Evaluation des propriétés immunomodulatrices de la bactérie lactique Lactobacillus plantarum NCIMB8826 dans le cadre de l'allergie aux acariens." Doctoral thesis, Universite Libre de Bruxelles, 2008. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/210414.
Full textAbstract:
Les effets anti-allergiques des bactéries lactiques sont suggérés par plusieurs études épidémiologiques, des essais cliniques et des modèles expérimentaux d’allergie. Cependant, les propriétés immunomodulatrices des bactéries lactiques sont sous-exploitées par les stratégies vaccinales développées pour combattre l’allergie et les mécanismes empruntés par ces bactéries pour moduler l’allergie restent peu caractérisés.<p>Dès lors, nous avons caractérisé les propriétés immunomodulatrices qu’exerce Lactobacillus plantarum NCIMB8826, une bactérie lactique modèle, sur la cellule dendritique étant donné le rôle déterminant de cette cellule sur la réponse allergique. Nous montrons que L. plantarum induit une forte sécrétion d’IL-12 p40, d’IL-12 p70, de TNF-a mais une faible production d’IL-10. Cette faculté à induire la sécrétion de cytokines polarisantes dépend de TLR2, de TLR9, de MyD88, de NF-kB, des MAPKs (en particulier JNK, p38 et ERK 1/2), de la composition de l’acide lipotéichoïque de L. plantarum et de CD14. Nous montrons aussi que l’ADN génomique de L. plantarum est un agoniste de TLR9 et que CD14 et CD36 facilitent la liaison de la cellule dendritique avec L. plantarum.<p>Ensuite, nous avons évalué le potentiel vaccinal d’une coadministration L. plantarum + Der p 1 dans un modèle murin d’allergie à Der p 1. Cette formulation vaccinale prévient la production d’IgE Der p 1-spécifique et atténue l’éosinophilie pulmonaire tout en stimulant une forte production d’anticorps IgG2a Der p 1-spécifiques et d’IFN-g par les cellules spléniques. Ces effets bénéfiques nous ont conduit à élaborer une bactérie lactique recombinante dérivée de L. plantarum produisant Der p 1 pour la vaccination contre l’allergie aux acariens. La forme antigénique que nous avons réussi à faire produire par L. plantarum correspond à une protéine de fusion entre la Maltose Binding Protein de E. coli et ProDer p 1 (le zymogène de Der p 1), la présence de ce partenaire de fusion étant indispensable à la production de ProDer p 1. En prophylaxie, la vaccination par cette bactérie recombinante prévient la production d’anticorps IgE-Der p 1-spécifiques et stimule la production d’anticorps IgG2a spécifiques, reproduisant les effets de la coadministration L. plantarum + Der p 1. Elle réduit de manière drastique la production d’IL-5 des cellules spléniques et des cellules ganglionnaires médiastinales et prévient l’éosinophilie pulmonaire mais n’a pas d’effet sur l’hyperréactivité bronchique. Der p 1 étant un des allergènes d’acarien les plus immunodominants, cet ensemble de données montre donc que cette bactérie recombinante constitue un vaccin prophylactique prometteur pour la prévention de l’allergie aux acariens. Des résultats préliminaires obtenus à partir de cellules dendritiques humaines et lymphocytes T autologues montrent la forte capacité de cette bactérie recombinante à induire le développement d’une réponse Th1 fortement polarisée (production d’IFN-g en l’absence de production d’IL-4 et d’IL-5), ce qui suggère que l’utilisation de cette bactérie recombinante pourrait être envisagée pour le traitement de l’allergie chez l’homme. <p><br>Doctorat en Sciences agronomiques et ingénierie biologique<br>info:eu-repo/semantics/nonPublished
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Michaud, Bénédicte. "Etude de la réponse lymphocytaire T dans l’allergie de l’enfant, au diagnostic et au cours de la désensibilisation." Thesis, Paris 5, 2013. http://www.theses.fr/2013PA05T025/document.
Full textAbstract:
Les maladies allergiques sont de plus en plus fréquentent. Elles atteignent souvent l’enfant jeune chez qui l’allergie respiratoire et l’allergie alimentaire sont les principales pathologies. L’unique traitement curatif est l’immunothérapie spécifique d’antigène (ITA), largement développée dans l’allergie respiratoire et encore à ses débuts dans l’allergie alimentaire. Pour adapter au mieux la prise en charge du patient, le diagnostic précis de l’allergie est indispensable et il n’existe actuellement pas d’examen biologique totalement fiable. Seul, la présence d’IgE spécifiques permet de diagnostiquer une sensibilisation à un allergène mais pas une allergie cliniquement symptomatique. Dans une première partie, nous avons étudié l’intérêt d’un test fonctionnel, l’ELISpot (Enzyme-linked immunosorbent spot), dans le diagnostic de l’allergie aux acariens chez l’enfant asthmatique. Le nombre de lymphocytes T circulants spécifiques d’acariens sécréteur d’interleukine (IL)-4 ou d’IL-13 était associé à la présence d’une allergie symptomatique, indépendamment des IgE spécifiques. Il était plus élevé dans le cas d’une rhinite allergique sévère et plus faible dans le cas d’une rhinite allergique légère. De plus, il variait au cours de l’année en fonction des saisons avec un pic en automne et un pic en début de printemps. Dans une deuxième partie, nous avons étudié l’intérêt de l’ELISpot dans le diagnostic de l’allergie au lait de vache chez l’enfant, confirmée par un test de provocation orale en double aveugle. Nous avons décrit que le nombre de lymphocytes T spécifiques de la caséine et sécréteurs d’IL-4 et d’IL-13 était associé à l’allergie au lait de vache avec une sensibilité de 100%. Par ailleurs, le nombre de lymphocytes T spécifiques de la caséine était également associé à la dose maximale de lait tolérée par l’enfant.Enfin, dans une troisième partie, nous avons étudié la réponse lymphocytaire T au cours d’une ITA sub-linguale (SLIT) d’une part et sous-cutanée (SCIT) d’autre part, chez des enfants asthmatiques allergiques aux acariens suivis pendant une année. Nous avons décrit une diminution des lymphocytes Th2 (sécréteurs d’IL-4 et IL-13) spécifiques d’acariens après 12 mois de SLIT associée à une augmentation des cellules sécrétrices d’IL-10 (Tr1) spécifiques d’acariens après 6 mois de SLIT. De plus, les lymphocytes T régulateurs (CD4+CD25hiCD127loFoxp3+) étaient augmentés après 12 mois de SCIT. Nous n’avons pas retrouvé de production accrue d’interféron γ (IFNγ) par les lymphocytes T spécifiques d’acariens au cours de la désensibilisation.Au total, ce travail nous a permis de décrire qu’un test fonctionnel, l’ELISpot, permet de réaliser un diagnostic fiable de l’allergie aux acariens et de l’allergie au lait de vache chez l’enfant. Par ailleurs, l’ITA induit une diminution des cellules Th2 et une augmentation des cellules Tr1 par voie sub-linguale ainsi qu’une augmentation des Treg Foxp3+ par voie sous-cutanée sans immunodéviation Th2/Th1, chez l’enfant allergique aux acariens<br>Allergic diseases are steadily increasing steadily and especially in children. Allergen specific immunotherapy (desensitization) is the only curative treatment for which accurate diagnosis of allergy is essential. Currently, the presence of specific IgE diagnoses a sensitization to an allergen but not a clinically symptomatic allergy. In a first part, we studied the value of a functional test, the ELISpot (Enzyme-linked immunosorbent spot) in the diagnosis of allergy to house dust mites (HDM). The number of circulating HDM-specific IL-4 and IL-13 secreting T cells was associated with the presence of symptoms, regardless of specific IgE and was higher in severe rhinitis than in mild rhinitis. In addition, it varied according to the season with a peak in autumn and a peak in early spring (wet periods with greater allergen exposure). In a second part, we studied the value of ELISpot for the diagnosis of cow's milk allergy in children, confirmed by double blind placebo control food challenge. We found that the number of casein-specific IL-4 and IL -13 secreting T-cells was associated with allergy to cow's milk. It was also inversely correlated to the cow’s milk tolerated cumulative dose. Receiver-operating characteristic (ROC) curve of combined IL-4 and IL-13 analysis was generated. AUC was 0,98 (95% CI 0.90-1.06). For a cut-off of 10 IL-4- and 12 IL-13 secreting T-cells, sensitivity and negative predictive value were 100%.Finally, in the third part, we monitored antigen specific T-cell response in HDM allergic children treated with sublingual ITA (SLIT) on the one hand and subcutaneous ITA (SCIT) on the other hand, during one year. We found a decrease in HDM specific Th2 cells after 12 months of SLIT associated with an increase in HDM specific IL-10 secreting T-cells after 6 months of SLIT. In addition, regulatory T cells (CD4 + CD25hiCD127loFoxp3+) were increased after 12 months of SCIT. In conclusion, this work has allowed us to describe a functional test, the ELISpot, as a reliable tool for the diagnosis of mite allergy and cow's milk allergy in children. In addition, in HDM allergic children, a decrease of Th2 cells and an increase of IL-10 secreting T-cells was found in children treated with SLIT to HDM as well as an increase in Foxp3+ Treg in children treated with SCIT
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Yi-Jing та 陳宜靖. "Effects of Perilla frutescens (Labiatae) extracts on house dust mite allergen Der p 2 induced inflammatory responses in bronchial epithelial cell BEAS 2B through inhibition of NF-κB / MAPK activation". Thesis, 2011. http://ndltd.ncl.edu.tw/handle/34328878899721215937.
Full textAbstract:
碩士<br>中山醫學大學<br>生化暨生物科技研究所<br>99<br>The Perilla frutescens Britt. (PF), a traditional Chinese medicinal herb, has been used in China for centuries to treat various diseases including bacterial and fungal infections and allergic diseases.House dust mites are well-known as a source of indoor aeroallergens and for causing allergic airway diseases. In this study, we investigated the anti-allergic effect of methanol extract of PF leaf (PFE) on Dermatophagoides pteronyssinus 2 (DP2)-stimulated bronchial epithelial cell line (BEAS-2B). Pro-inflammatory cytokines mRNA expression was analyzed by RT-PCR and quantitative real-time PCR. Activation of kinase cascades were investigated by immunoblot. Our results revealed that PFE significantly reduced the DP2-induced mRNA expression of IL-4, IL-5, IL-6, IL-8, IL-13, MCP-1 and GM-CSF in a dose-dependent manner. A mechanism based kinase cascades analysis showed that PFE suppressed the DP2- induced phosphorylation of mitogen-activated protein kinase (MAPK) including extracellular signal-regulated kinase1/2 (ERK1/2) , P38 MAPK(p38) and c-Jun N-terminal kinase (JNK).Additionally,we also found that PFE inhibited the translocation of NF-κB/p65 into nucleus which may result from the suppression of IκBα degradation. Taken together, these findings indicate that PFE significantly alleviates DP2-induced inflammatory responses, which may attribute to suppression of MAPK signaling and nuclear of NF-κB, suggesting that PFE should be beneficial to treatment of allergen-induce inflammation.
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Helen-Sun and 孫海倫. "Prevalence and Distribution of House Dust Mite in Taichung." Thesis, 1999. http://ndltd.ncl.edu.tw/handle/40477525379427450654.
Full textAbstract:
碩士<br>中山醫學院<br>醫學研究所<br>87<br>Abstract
Many studies including skin test and radioallergosorbent test (RAST) strongly suggested that house dust mite are the most important allergen in Taiwan, especially in asthmatic children. Taiwan is a subtropical island. The high temperature and relative humidity throughout the year and overcrowding are ideal condition for mite growth. There were few reports about distribution of mite in Taiwan especially in Taichung.
We collected every two months between June 1998 and May 1999 from eight houses of mite-allergic patients and four normals. About 1m surface area, each of living room, floor, sofa, bed room and mattress was vacuumed for one minute. The floating method was used to collect mites in dusts and the mites were countered under stereo-microscope. House dust mites species were identified, too.
The results showed the following five points. First, the average number of mites in patients’ houses was not different that in normal’s houses. Second, the total mite amounts were greater in July to November and lowest in January. It was compatible with the lowest temperature in January with constant relative humidity all the year. Third, the mattress was the largest mite amount among four different vacuumed areas. Forth, the total amount of mites was not correlated with the frequency of asthma attacks. Fifth, the Dermatophagoides pteronyssinus (DP) was the dominant specie (77%) of house dust mites found in Taichung and Dermatophagoides farinae (DF) was the second (13%).
Environmental control of house dust mites is the first line management for mite-sensitive asthmatic patients. Mattress is a very important area to reduce the mite amounts according to our study.
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Chen, Yi-Chieh, and 陳怡潔. "Airborne House Dust Mite Allergen in School Children’s House in Kaohsiung City." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/40437564203283166786.
Full textAbstract:
碩士<br>高雄醫學大學<br>公共衛生學研究所<br>100<br>Mite is the Arthropods. The size is about 300~500μm. High temperature and relative humidity provides appropriate environmental conditions for house dust mite. The body, excrement, egg, and corpse may lead the hypersensitivity of people. The environmental condition in South Taiwan is very hot and wet, so the climate suit house dust mite to growth. Some references clearly show that the house dust mite allergen may lead the respiratory tract diseases. In addition, children are the sensitive population. Therefore, the purpose of the present study is to evaluate the relation between mite in household dust and air and schoolchildren’s lung function. We collected questionnaires about children’s allergy situation. The house dust mites’ allergen – Der p 1 in both air and dust samples were collected by a vacuum cleaner for dust samples and filters for air samples, respectively. In addition, we measured children’s lung function.
The average concentration of living room floor dust samples, kitchen floor dust samples, bedroom floor dust samples, pillow surface dust samples, and bed surface dust samples was 42 ng/g, 77 ng/g, 159 ng/g, 1581 ng/g, and 3989 ng/g, respectively. For air samples, the average concentration of living room and bedroom air samples was 0.01 ng/m3. For the concentration of jumping bed air samples, the average value was 37 ng/g, which was about 3700 times of ordinary level. To define children allergy condition according to the IgE level, we found that the Der p 1 concentration of pillow surface dust samples and bedroom air samples of allergic children was significantly higher than that of non-allergic children. The Der p 1 concentration of bedroom air samples was significantly decreased children’s lung function (FVC, FEV1, FEV3, PEF).
In conclusion, our study provided the concentration distribution of house dust mite allergen in schoolchildren‘s house. We observed higher concentration of pillow surface dust samples and bedroom air samples of allergic children than that of nonallergic children. In addition, house dust mite allergen significantly decreased lung function of children.
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Du, Plessis Jan Leonard. "The efficacy of house dust mite 30CH in ameliorating the symptoms of dust allergy." Thesis, 2009. http://hdl.handle.net/10210/2854.
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Wei, Jun Jie, and 魏俊傑. "Purification and characterization of house dust mite allergens from dermatophagoides pteronyssinus." Thesis, 1994. http://ndltd.ncl.edu.tw/handle/83577236508202833461.
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LI, YUE-LUN, and 李岳倫. "Prodtction and characterization of human monoclonal antibldies to house dust mite allergens." Thesis, 1992. http://ndltd.ncl.edu.tw/handle/77945870527725754135.
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Chang, Hao-Chen, and 張皓禎. "Generation and characterization of human monoclonal antibodies against house dust mite allergen." Thesis, 1993. http://ndltd.ncl.edu.tw/handle/86943343810880714523.
Full textAbstract:
碩士<br>國立臺灣大學<br>微生物學系<br>81<br>House dust mite Dermatophagoides pteronyssinus is one of the
most important allergens causing allergic asthma in Taiwan. To
study this allergen, we have first established an early cloning
system for Epstein-Barr virus transformed lymphoblastoid cell
line (EBV-LCL), and produced a panel of human monoclonal
antibodies against houst dust mite allergen (Dermatophagoides
pteronyssinus) from both normal individual and dust mite
allergic patients through ELISA screening. We then
characterized these human monoclonal antibodies by analyzing
the physical, chemical and immunologic natures of allergens. We
have successfully established several mite-specific EBV-LCL
secreting human antibodies of various isotype. Immunoblot
analysis showed that WJL-B and LCB-E recognized about 14~15 kD
band, WJL-C and WJL-D recognized about 25~30 kD of mite
allergens. This antibodies are cross-reacting against allergens
from various insects but did not cross-react to other
allergens. Epitope analysis show that they are non-
conformational dependent protein . These results indicate that
the immune response of human and murine to allergens are
different.Establishment of a panel of mite allergen specific
human monoclonal antibodies will help to identified the epitope
and structure components of different sources of insect
allergens, the VH gene usage of these EBV-LCL. This may direct
to understand the pathogenic, physiological phenomenon and
therapy in allergic patient.
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Hsu, Ya-hui, and 許雅惠. "Prediction of the Level of House Dust Mite Allergen by Residential Characteristics." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/74063395926884594468.
Full textAbstract:
碩士<br>國立臺灣大學<br>職業醫學與工業衛生研究所<br>97<br>The prevalence of atopic eczema and asthma in adolescents has been reported to be increasing in the past decades in Taiwan. Exposure to house dust mite (Dermatophagoides pteronissinus) is known to play a potent role in the onset and aggravation of allergic diseases, such as atopic dermatitis and asthma. In Taiwan, high temperature and relative humidity provide favorable conditions for dust mite to grow. It is important to evaluate the level of Der p 1 and environmental predictors, especially the residential characteristics. In addition, previous studies on dust mite allergens in Taiwan were mostly sampled by investigators, we tested the feasibility and comparability of sample collection by adult residents in this study.
We studied 46 homes in the 6 cities and counties in Taiwan, included Taipei, Chiayi, Yulin, Tainan, Kaohsung and Taitung. Among these cities, 38 were the participants of the Taiwan Birth Cohort Pilot study, and their residential characteristics were studied during July to September 2007. The other 8 participants were choosed according to a respiratory study during August 2008 in Sinchuan. The information of environmental conditions, structure of house or apartment, pet-owning, habits, and frequency of cleaning were collected by questionnaire. For the comparison of resident- and investigator-collected dust mite samples, the residents sampled the dust from the surface of mattress by vacuum cleaners according to the investigator’s written sampling direction. The investigator also sampled the dust from the floor of the living room and child’s bedroom, mattress, and pillow. The dust mite allergen (Der p 1) was measured using a two-site monoclonal antibody enzyme-linked immunosorbent assay (ELISA).
Among the 46 homes, the geometric means of Der p 1 were 0.13 μg per gram of dust (μg/g) for the floor of living room, 0.31 μg/g for the floor of children room, 1.70 μg/g for the mattress, and 2.90 μg/g for the pillow. By paired t test, the Der p 1 levels sampled by adult residents was not demonstrated statistically different from those sampled by investigators, moreover, the results sampled by residents and investigators were highly correlated (r=0.75). Higher dust mite levels were associated with having water leakage, dog-owning, observed surface molds, and longer duration after the last cleaning. The usage of air conditioner was negatively associated with level of dust mite
This study provides the background levels of dust mite allergen (Der p 1) in Taiwan 6 cities and the residential environmental factors were found to play an important role in mite allergen concentrations. Future study on the effects for reducing mite allergen by environmental modification will be warranted.
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Wan, Gwo-Hwa, and 萬國華. "SEASONAL VARIATIONS OF HOUSE DUST MITE (Der P I) IN TAIPEI AREA." Thesis, 1993. http://ndltd.ncl.edu.tw/handle/03009483135885292848.
Full textAbstract:
碩士<br>國立臺灣大學<br>公共衛生學研究所<br>81<br>In Taiwan ，one of three children have related allergic
diseases，such as asthma，rhinitis，and so forth． It is found
that house dust mite（ HDM ）is the most important allergen in
Taiwan ， especially for asthmatic children． Moreover
， Dermatophagoides pteronyssinus（ Dp ） was recognized as
the dominant species of HDM in the Taipei areas． However ，
the characterization of HDM antigens and HDM antigen-
associated aerosols has not been evaluated ，
especially the size distributions of antigen-related
particles which determine the deposition behavior of inhaled
HDM antigen in the lung ．The objective of this
investigation was to assess the Der p I amounts of surface
samples in the houses of twenty-four HDM- allergic children
and of six normal persons． Dust samples collected from the
homes of 24 asthmatic children and 6 normal persons from June
1992 to April 1993 were observed to contain Der p I
concentrations． The highest concentrations were observed
in November（ geometric mean， 16,986 ng/g dust and median
，17,053 ng/g dust ）and December（ geometric mean，20,119 ng/g
dust and median，43,672 ng/g dust ），but the lowest one
occurred in February（geometric mean ，165 ng/g dust and median
，143 ng/g dust）．In regard to Der p I concentrations in
the homes of the asthmatic children and the normal persons
，the levels in asthmatic children's homes were higher
than those observed in normal persons' houses except
February． Moreover ，it was found that there were
significant concentration differences in the mattress（ p =
0.01） and bedroom floor（p = 0.004）in November between these
two groups' homes.
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Hsu, Ming-Yi, and 許名宜. "Studies of intradermal tests of house dust and dust mite in canine atopic dermatitis at Taipei area." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/35091847924706999255.
Full textAbstract:
碩士<br>國立臺灣大學<br>獸醫學研究所<br>93<br>Host dust and dust mite are important allergens in canine atopic dermatitis. The purpose of this study was to investigate sensitivity to house dust and dust mite in atopic dogs in Taipei city. One hundred atopic dogs were enrolled according to history, clinical signs ,physical and dermatological exams. Intradermal test with house dust and dust mite were performed and investigated. The diagnosis of atopic dermatitis of each dog was based upon the clinical manifestations compatible to criteria, and results of intradermal skin tests. fifty females and fifty males aged from one to forteen years old with twenrt-three breeds . The mean age of this series was 5.7years. Among these One hundred dogs, eighty six dogs (86%) exhibited positive reactions to either house dust or dust mite, or both. Positive reactions to house dust and dust mite were 15% (15/100) and 3% (3/100) respectively. Positive reaction to both allergens was 68% (68/100). No breed, age predilection found in this series. Sex and body weight were statistical related to positive reaction (either house or dust mite), Analyses between sex, age, body weight and positive reaction to house dust, positive reaction to dust mite, and positive reaction either to house dust or dust mite reveals statistically related between body weight and positive reaction. Percentage of cases exhibited positive reaction to both allergens was significantly higher than that of cases reacted to either allergen alone. This suggested that positive reaction to either allergen would be associated to each other. Further investigation would be needed to clarify the cross-reaction between house dust and dust mites. This study suggests the high positive rate of intradermal tests to house dust and dust mite. House dust and dust mite are common and important allergens in atopic dogs at Taipei area.
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Chen, Chih-Long, and 陳志隆. "House dust mite induces allergic sensitization and inflammation by activating the innate immunity." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/88205774312634115174.
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博士<br>國立成功大學<br>基礎醫學研究所<br>93<br>Allergic asthma is a chronic airway inflammation, which is characterized by the infiltration of Th2 cells and eosinophils in the airways, airway hyperresponsiveness, and excessive mucus secretion. Dermatophagoides farinae (Der f) is one of the most prominent and important species of house dust mite implicated in allergic asthma. However, the allergenicity of Der f is not fully known. The first part of the thesis was to investigate whether the effect of Der f on innate immunity involved in allergic sensitization in a murine model of asthma with emphasis on alveolar macrophages (AMs) and mast cells (MC). In vivo results showed that Der f induced eosinophilic airway inflammation and allergen-specific antibody in mice after repetitive challenge. Administration of sodium cromoglycate, a mast cell stabilizer not only suppressed acute mast cell activation as revealed by the release of mMCP-1 but also attenuated the allergic airway inflammation. There was a 15- to 25-fold increase in nitric oxide (NO) and a 50–75% decrease in antioxidant concentrations in bronchoalveolar lavage fluids (BALF) of Der f mice. AM from Der f-challenged mice expressed enhanced levels of costimulatory molecule B7 and augmented T cell proliferation ex vivo. Furthermore, dexamethasone attenuated and live RSV infection augmented airway inflammation and sensitization in mice repetitive challenge with Der f. In vitro studies showed that Der f activated NF-�羠 of AM and, unlike ovalbumin (OVA) or lipopolysaccharides (LPS) stimulation, it up-regulated IL-6, TNF-��, and NO. In addition, Der f down-regulated antioxidants. Der f-stimulated AM expressed enhanced levels of B7 molecules, supported T cell proliferation, and promoted Th2 cell development. Arg-Gly-Glu-Ser (RGDS) peptide and neo-glycoproteins blocked Der f effects on AM. Der f but not OVA could rapidly induced an enhanced expression of the genes for IL-1��, IL-4, IL-6, IL-9, IL-13 and TNF-�� in mystocytoma P815 cells, and stimulated P815 cells and bone marrow-derived mast cells (BMMC) to produce IL-4, IL-6 and TNF-�� in a dose- and time-dependent manner. Cycloheximide blocked the Der f-induced IL-4 production, indicating a de novo protein synthesis process. Furthermore, supernatants from Der f-stimulated mast cells not only chemoattracted monocytes and T lymphocytes but also up-regulated the expression of B7.1 molecule, eotaxin, RANTES, MCP-1, and IP-10 mRNA of AM; they supported PHA-induced T cells proliferation and promoted Th2 cell development. These results showed that AM and mast cells in the airway mucosa might facilitate the initiation and development of allergic sensitization and inflammation.
The second part of this thesis was intended to identify, quantify and characterize the BALF proteins related to the Der f-induced allergic airway inflammation using two-dimensional electrophoresis (2-DE) and mass spectrometer. Twenty four hours after a single Der f challenge, there was a significant increase in the number of silver-stained spots mostly in an area of the electropherogram between pI 5-7 and Mr <25 kDa. The intensity, and, in a lesser extent, the number of protein spots were gradually increased and sustained for at least 48 h, which was correlated with a cellular influx into the BALF. Der f-, OVA-, and LPS-challenged mice had a total of 276, 231, and 251 spots detected, and among them 82, 56 and 37 spots had a twofold increase in abundance, respectively. Image analysis showed that the majority of the spots in the three treatments were overlapped (82% of Der f, 97% of OVA and 90% of LPS). There was a subset of the induced spots specific to each allergen, 24 spots for Der f, 9 for OVA and 10 for LPS. Fifteen proteins were identified in the BALF samples of single Der f-challenged mice by mass fingerprinting. They were proteins involved in acute phase response, host defense, antioxidant defense, cellular structure, and other purpose. In the BALF of repetitive challenged mice, 224 spots were detected. Most of which clustered in an area of the electropherogram between pI 5-7 and Mr 25-50 kDa. Comparison of the BALF protein maps of the two groups showed 56 spots with significant variations in relative abundance. Among them 28 spots with greater than twofold increase in intensities were subjected to MS, and they were proteins related to inflammation, host defense, enzymes involved in oxidant and antioxidant balance, cytoskeleton and antibody. Collectively, this study provides a large-scale profile of protein expression in the lung of Der f-induced inflammation in mice. 2-DE/MS analysis in combination with cellular immunoassays will give a more deep insight into the disease mechanisms of allergic asthma.
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Chen, Chia-Yu, and 陳佳郁. "House dust mite in air and dust samples in school children''s houses in Kaohsiung City." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/72688247963821862721.
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碩士<br>高雄醫學大學<br>職業安全衛生研究所<br>99<br>According to the previous studies, house dust mite is the primary source of antigen in indoor environment. For children, sensitization to house dust mite allergens is strongly associated with asthma.
There are four objectives of this study. First one, we want to investigate the concentrations of house dust mite allergen of asthma and non-asthma school children’s houses in house dust and air in Kaohsiung city. Second, we plan to evaluate the correlations of dust and air samples. Third, we try to assessment the correlations between mite in dust and air samples and health effects. And the last one is to assessment the correlations between mite in dust and air samples and other factors.
From April to October in 2010, we collected 240 dust samples and 120 air samples from 60 schoolchildren’s houses in Kaohsiung city. We used vacuum cleaner and cellulose extraction thimbles to sampling dust from living room floor, bedroom floor, mattress and pillow. And we used a pump and Teflon filters to sampling the concentration of Der p 1 antigen in air in bedroom for 24 hours. We also investigated some environmental factors in housed, like the concentration of fungi, bacteria, endotoxin, and temperature etc. Besides, we evaluated lung function, exhaled CO and specific IgE from children.
In dust samples, the detected rates of Der p 1 antigen in living room floor samples was 67%, in bedroom floor samples was 84%, in mattress samples was 98% and 93% in pillow samples. The detected rates of air samples were both 57% in living room and bedroom.
The concentration of Der p 1 antigen in bed samples (the mean concentration of mattress and pillow samples) (1817.83 ng/g) was significant higher than floor samples (the mean concentration of living room floor and bedroom floor samples) (240.59 ng/g), p &lt; 0.001.
Whether it is the first day, the next day or two days on average, VC% of children were significant correlation with the concentration of Der p 1 antigen in bedroom samples in air. Children who allergic with house dust, the concentration of Der p 1 antigen in their bedrooms in air samples was significant higher than non allergic children.
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Wu, Jiangping. "Investigation of the existence of anti-idiotypic antibodies in house dust mite allergic individuals." 1986. http://hdl.handle.net/1993/15500.
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MINH, NGUYEN HOANG, and 阮黃明. "Blo t19, a Novel Antimicrobial Peptide Isolated From The House Dust Mite Blomia tropicalis." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/41197297694126624946.
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碩士<br>國立清華大學<br>分子醫學研究所<br>99<br>Abstract
The discovery of novel antimicrobial peptides plays an important role in the development of new antimicrobials for treatment of multidrug-resistant bacteria. Antimicrobial peptides are relatively small (12 to 100 amino acids), amphipathic, and positively charged molecules of variable length, sequence and structure with activity against a wide range of microorganisms including bacteria, protozoa, fungi, viruses and even tumor cells. They usually act through relatively non-specific mechanisms resulting in membranolysis but can also stimulate innate immune responses. Several antimicrobial peptides have already entered pre-clinical and clinical trials for the treatment of catheter site infections, cystic fibrosis, acne, wound healing and infections in patients undergoing stem cell transplantation. Previously it has been shown that Blo t19 - one of the allergens isolated from Blomia tropicalis exhibits weak homology with a characterized antimicrobial peptide ASABF. The aim of this study is to investigate whether Blo t19 also possesses an antimicrobial activity. In the present study, Blo t19 was overexpressed in E. coli BL21 and Origami strains and purified to homogeneity. Although the expression level of recombinant Blo t19 in E. coli BL21 was higher than in the Origami strain, the Origami-synthesized Blo t19 seemed to be more active and stable than that produced in BL21 and was therefore used for all subsequent studies. Recombinant Blo t19 is able to inhibit 50% of Pseudomonas aeruginosa growth at 0.4 µg/ml and kill 99% of P. aeruginosa at 50 µg/ml in 2 h incubation. The antibacterial activity was dose-dependent and could be inhibited by CaCl2. Moreover, this peptide did not exhibit detectable cytotoxicity to cultured mammalian cells. In conclusion, we demonstrated in this study that Blo t19 is a novel antimicrobial peptide and has the potential to be developed into a new drug for the treatment of P. aeruginosa infections.
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Mueller, Geoffrey Andrew. "The molecular and antigenic structure of the major house dust mite allergen Der p 2 /." Diss., 1998. http://wwwlib.umi.com/dissertations/fullcit/9916261.
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Chung, Chia-Chang, and 張家禎. "Study on expression of the house dust mite allergen Der p 2 in Flammulina velutipes." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/48467094406231182795.
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碩士<br>國立臺灣大學<br>微生物與生化學研究所<br>94<br>House dust mites are the most significant indoor allergens. Dermatophagoides pteronyssinus group 2 protein (Der p 2), originated from the digestive tract of host dust mites, plays a major role in induction of asthma, allergic rhinitis and allergic dermatitis. Up to 85% of asthma patients are sensitized to Der p 2 in Taiwan. There are 387 bp in the cDNA clone which encodes a 129-aa protein with a molecular mass of 14 kDa.
Oral tolerance can induce a group of antigen-specific regulatory T cells to suppress antigen-specific immune response through the oral administration of protein antigens presented by intestinal lymphoid tissue. However, the application of oral tolerance for the treatment of allergey was limited due to complicated purification and high cost of candidate allergens. To solve this problem, this study would like to develop the Der p 2-transgenic mushrooms possessing advantages of conveniently oral delivery and inexpensive protein production process.
In this study, four different strategies were used to improve the heterogenous gene expression in Flammulina velutipes. Plasmids with an intron at 5’ of der p 2, the endoplasmic reticulum retention signal HDEL at 3’ of der p 2, fusion gene of der p 2 and egfp (enhanced green fluorescent protein), and modified the codon of der p 2 (mder p 2) were constructed for F. velutipes. After transforming the target DNA to mycelium by eleporation, only transformants of mder p 2 with an obvious signal were observed in Western blot analysis. Using Der p 2 sandwich enzyme-linked immunosorbent assay (sandwich-ELISA), the transformant pAImDS-19 expressed the highest Der p 2 protein, c.a. 0.02% of the total soluble proteins. This study demonstrated the expression of mite allergen in mushroom and suggested that the codon usage or the G + C content are crucial for the expression of heterogenous protein in F. velutipes.
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Lai, Ying-Siou, and 賴盈秀. "The Impacts of In-house Airborne Dust Mite and Other Factors on Maternities and Neonatals." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/24559266359011375952.
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碩士<br>高雄醫學大學<br>公共衛生學系公共衛生學碩士班<br>104<br>Background: Dust mite allergen has been known that it may cause sensitization and allergic diseases. World Allergy Organization (WAO) estimated there were about 40 percent of whole population suffered from allergic diseases around the world. Studies in Taiwan also indicated that more and more children suffered from allergic diseases in 2005. However, most studies have said that children exposed to dust mite allergen in their infancy may increase the risk of allergy. Thus, this study sampled in house areas which pregnant women and six months old babies lived and then evaluated the concentrations of Dust mite allergens and other effective factors in Kaohsiung city.
Materials and methods: This study recruited 34 pregnant women from the OBGYN department of a medical center in Kaohsiung, and then investigated the concentrations of house dust mite allergen inside the dust and air in their houses while they were at 28-40 weeks of pregnancy or their babies were at six months after childbirth. Dust samplings were conducted at living rooms, kitchens, bedrooms and the mattresses and the pillows on which mothers and babies slept. Air samplings were conducted at living rooms, bedrooms and balconies. The concentrations of Dust mite allergens (Der p1 and Der f1) were investigated by using microplate ELISA reader.
Results: In dust samplings, Der f1 had higher positive rate than Der p1. However, the concentrations of Der f1 were lower than the concentrations of Der p1. In air samplings, whether positive rate or concentrations were Der p1 higher than Der f1. Comparing Dust mite allergens with other environmental factors showed that there were significant differences between sweeping frequency in bedroom and the concentrations of Der f1 (p < 0.05). There were significant differences between the concentrations of Der f1 at floors of kitchens in different household patterns (p < 0.05). There were significant differences between cleaning frequency of beddings and the concentrations of Der p1 (p < 0.05). The correlation comparing of the concentrations of Der f1 in living rooms respectively showed that there were significant differences to bedrooms (r = 0.85, p < 0.01), balconies (r = 0.66, p < 0.01)and the total air samples (r = 0.99, p < 0.01). Furthermore, there were significant differences between the concentration of Der f1 in the air of balconies and the concentrations of Der f1 in the dust of mattresses. (r = 0.70, p < 0.05).
Conclusions: This study applied the concentration distribution of household environmental Dust mite allergens whether dust samples or air samples. And this results showed that there were different trends between dust samples and air samples. Even though previous studies used dust samples as the Dust mite allergens exposure, this results revealed that the concentrations of dust samples might not represent the true exposure of Dust mite allergens.
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Hynes, Tyler. "Role of caveolin-1 in airway hyper-responsiveness and inflammation in response to house dust mite challenge." 2012. http://hdl.handle.net/1993/6206.
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Allergic asthma is a syndrome characterized by respiratory distress in response to environmental triggers. This atypical response to an allergen is an over reaction of the immune system causing an influx of inflammatory cells into the airway and concomitant airway smooth muscle constriction. Firstly, we demonstrate using whole house dust mite (HDM) extract as a sensitizing allergen produces an equivalent or more robust hyperresponsive and inflammatory reaction than can be achieved with the widely used ovalbumin (OVA) sensitization / challenge protocol. Secondly, we investigated the role of caveolin-1 in the pathophysiology of allergic asthma . Our data suggest an important role for cav-1 in down regulating allergic airway inflammation, leading to reduced airways hyperresponsiveness and mucus overproduction.
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TSAI, YU-LIN, and 蔡育霖. "Zn Sulfate improve unbalance T cell subtypes of House dust mite-allergic asthmatic patients---ex vivo study." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/35khyj.
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碩士<br>弘光科技大學<br>營養醫學研究所<br>104<br>Asthma is the common chronic inflammatory disease of the airways characterized by variable and recurring symptoms, reversible airflow obstruction, and bronchospasm. In the pathogenesis of asthma, the imbalance of helper T (Th) 1/Th2 and Th17/ regulatory T cell cells are believed to establish the asthmatic inflammatory response. Nutritional intervention is an important tool to decrease the severity of asthmatic disease. The aim of this study is to investigate the beneficial role of zinc supplementation to affect T cells profiles and cytokines production.
36 house dust mite (HDM) allergic asthmatic patients and 31 healthy subjects were enrolled. Peripheral blood mononuclear cells (PBMCs) were collected from two groups. The recombinant D. pteronyssinus (Der P) antigen with or without zinc sulfate (25μM or 50μM) stimulated PBMCs for 48 hours. The cells surface markers and intracellular cytokine markers of T cells were measured by flow cytometer. The inflammatory factors in plasma and culture supernatant were measured by ELISA.
Zinc sulfate (25μM or 50μM) reduced the levels of Th2 and Th17 cells, but increased the levels of Th1 and Treg cells. Zinc sulfate also reduced levels of interleukin (IL)-4 and IL-17, but increased the levels of IFN-γ. In this study, we found the beneficial effects of zinc on allergic airway inflammation. The zinc sulfate improved unbalanced T cells profiles and might be a potential therapeutic for airway inflammation.
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Pei-ChiChen and 陳佩琪. "Water soluble chitosan inhibits nerve growth factor in murine model of house dust mite induced allergic rhinitis." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/93145337664130719056.
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碩士<br>國立成功大學<br>微生物及免疫學研究所<br>102<br>Allergic rhinitis (AR) is a disease with symptom of nasal airway hyperresponsiveness and mucosal inflammation mediated by IgE-associated processes. Nerve growth factor (NGF), a neurotrophin, has been shown to play an important role in neuroimmune responses by augmenting an existing type 2 T helper cell (TH2) immune response. Since chitin exhibits anti-inflammatory properties by inhibiting the development of allergic TH2 response, we aimed to assess the effect of the soluble derivatives of chitin—water soluble chitosan (WSC) on the NGF in a mouse model of Dermatophagoides pteronyssinus (Der p)-induced AR. First, we established an NGF-mediated AR toward augmenting systemic total and Der p-specific IgE levels, upper airway hyperresponsiveness, and local TH2 related immune response including the infiltration of eosinophils and degranulation of mast cells as well as TH2 related cytokines production in the nasal septum and nasal cavity lavage fluids. Interestingly, intranasal administration of WSC reduced allergic inflammation and improved the upper airway hyperresponsiveness. The expression of NGF and its related low affinity p75 neurotrophin receptors (p75NTR) as well as high affinity tyrosine kinase receptor A (TrkA) recptors in nasal epithelium of Der p-stimulated mice also repressed. Next, we used human nasal septum epithelial cell line (RPMI-2650) to investigate the detail mechanisms of candidate anti-allergic agents—WSC in attenuating Der p-induced airway inflammation. The results showed that NGF and TH2 related cytokines create an amplification loop resulting in broader allergic inflammation in upper-airway epithelial cells. In addition, WSC attenuated allergic inflammation and the epithelial cells damage through inhibiting NGF biosynthesis during allergic TH2 immune responses. In summary, we have demonstrated the role of NGF in a mouse model of house dust mite-induced AR, and the therapeutic effect of WSC in our AR mouse model, may through the attenuation NGF-induced airway inflammation as well as the inhibition of NGF synthesis. Our finding provides a new therapeutic modality of patients suffered with AR in clinical condition.