Academic literature on the topic 'Housefly – Control'

Piperonyl butoxide (PBO), a methylenedioxyphenyl compound, is primarily used as a synergist in combination with space spray, residual and admixture products for the control of insect pests in or around domestic and commercial premises, especially food preparation areas. Also, tetramethrin is known as a knockdown agent on target organism and it is generally used with piperonyl butoxide. In this study, effects of piperonyl butoxide and tetramethrin combinations on biological activities of synthetic pyrethroids, cypermethrin, deltamethrin, and permethrin against different housefly (Musca domestica Linnaeus, 1758) populations were evaluated. In addition, the biological efficiency of the insecticides used in the study, insecticide + PBO and insecticide + PBO + tetramethrin combinations, against the WHO standard sensitive housefly population and housefly populations collected from different parts of Turkey were compared. Results showed that PBO extensively promoted the ratio of knockdown and killing effect values of the insecticides. The results also indicated that PBO and PBO + tetramethrin combinations moderately reduced the knockdown effect times of all formulation in all housefly populations. The knockdown effect times were more decreased by insecticide + PBO + tetramethrin combinations than insecticides that are used alone and insecticide + PBO combinations.

In the housefly Musca domestica, synthesis of yolk proteins (YPs) depends on the level of circulating ecdysteroid hormones. In female houseflies, the ecdysterone concentration in the hemolymph oscillates and, at high levels, is followed by expression of YP. In male houseflies, the ecdysterone titre is constantly low and no YP is produced. In some strains, which are mutant in key components of the sex-determining pathway, males express YP even though their ecdysterone titre is not significantly elevated. However, we find that these males express a substantial amount of the female variant of the Musca doublesex homologue, Md-dsx. The dsx gene is known to sex-specifically control transcription of yp genes in the fat body of Drosophila melanogaster. Our data suggest that Md-dsx also contributes to the regulation of YP expression in the housefly by modulating the responsiveness of YP-producing cells to hormonal stimuli.

Insects are an attractive alternative to fish meal (FM) as a sustainable protein source in aquaculture feed that does not negatively impact the marine ecosystem. Despite housefly (Musca domestica) larvae having adequacy of amino acid profiles, they have sometimes been reported to be inferior to FM, especially for marine carnivorous fish species. Here, we report that the removal of the hydrophobic fractions from housefly larvae enables significant replacement of FM in the diet of the red seabream (Pagrus major). In a feeding trial, housefly (HF) larvae that had the hydrophobic fraction removed as a complete substitution for 70% FM produced satisfactory growth. However, HF larvae that were supplemented with the hydrophobic fraction resulted in significant growth reduction. Growth recovery was incomplete by supplementation of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) to undefatted HF larvae, being equivalent to that of fatty acid content with a control diet. Moreover, fish with a dietary intake of catechol identified from the hydrophobic fraction of the HF showed growth reduction and morphological alterations in the intestine. Our findings indicate that the hydrophobic fraction from HF larvae contains a negative factor for fish growth and eliminating the fraction from HF larvae is thought to be an important process for sustainable aquaculture.

ABSTRACT Background Allergic rhinitis is a common IgE-mediated inflammatory condition of the nasal mucosa. Skin prick test (SPT) remains the most useful test for detecting specific allergic triggers associated with allergic rhinitis. Objective Our objective was to study the sensitivity to common aeroallergens in allergic rhinitis patients using SPT Materials and methods We conducted SPT to 65 common aeroallergens on 100 patients more than 12 years of age with symptoms suggestive of allergic rhinitis. Results Sixty-one patients (61%) tested positive for at least one aeroallergen, of which, 54 patients (88.52%) showed polysensitization while seven patients (11.48%) showed monosensitization. The most common aeroallergen group implicated in SPT positivity was insects (48%); housefly (34%) being the most commonly implicated insect. Conclusion This study highlights a high prevalence of sensitivity to aeroallergens in the Indian scenario. It stresses upon the fact that environmental control measures might still prove to be the mainstay of treatment in our scenario, owing to housefly being the most commonly implicated aeroallergen. How to cite this article Mishra M, Chakravarti A, Kumar R. Skin Sensitivity to Aeroallergens in Allergic Rhinitis. Clin Rhinol An Int J 2013;6(2):64-66.

Thesis (PhD)--Stellenbosch University, 2004.
ENGLISH ABSTRACT: In surveys carried out on poultry farms in the Western Cape Province, South Africa, the larvae of the flies Musca domestica L., Fannia canicularis (L.) and Leptocera sp. were found to be numerous. The majority of M domestica larvae and the mite Proctolaelaps sp. were recovered from manure with temperatures between 20 and 26°C while the larvae of F. canicularis and Leptocera sp. were mostly found between 14 and 22°C. Predominant predatory arthropods in the manure were the histerid Carcinops pumilio (Erichson), the staphylinid Philonthus sordidus (Gravenhorst) and the mite Macrocheles muscaedomesticae (Scopoli). These had a manure temperature tolerance of between 12 and 31°C. Macrocheles muscaedomesticae and P. sordidus preferred fresh manure, whilst C pumilio preferred aged manure. Therefore these two predators could complement one another in integrated pest management programmes. From a study of the vertical cross-sectional profile of the manure cone it was clear that the larvae of the prey species M domestica and F. canicularis and predator P. sordidus distinctly occupy the crest of the manure cone. The succession of arthropods in accumulating poultry manure and the effect of manure height on their numbers was studied. The prey species M domestica and F. canicularis were the first colonizers followed by the predators P. sordidus and M muscaedomesticae. Carcinops pumilio was a late colonizer. The numbers of M domestica and F. canicularis larvae were negatively correlated with the increase in manure height whilst the numbers of the predators C pumilio and M muscaedomesticae were positively correlated with this. The total developmental time of C pumilio from egg to adult emergence at 30°C was 20.5 days. Two larval instars were recorded. The immature stages sustained about 50% mortality before adult emergence. Carcinops pumilio adults can live up to 130 days at 30°C and had a Type I survivorship curve. The intrinsic rate of natural increase (rm) was 0.064943 and net reproduction rate (Rs) was 20.191.The rate of oviposition was directly proportional to body size and weight. Increase in density had a dampening effect on fecundity and led to an increase in developmental time. Thus density will be a critical factor in any mass rearing programme for this predator of fly larvae. In addition, an increase in density stimulated the dispersal of C. pumilio. A crowding level of 50 C. pumilio adults per 200ml container resulted in the least dispersal which did not exceed 2.5% per day, compared with up to 24.0% at a crowding level of 400. The predation rate of flies by C. pumilio in the laboratory decreased with an increase in predator density, but increased with an increase in starvation. Starvation had no effect on predation rate of M muscaedomesticae. A technique exploiting Drosophila melanogaster Meig. as a source of prey was successfully developed for rearing C. pumilio, which could be of commercial value. Drosophila melanogaster appeared to be an ideal candidate as a source of prey for mass rearing C. pumilio due to its short developmental time of about ten days. It is also inexpensive and easy to breed, and has a very high biotic potential. Carcinops pumilio that were fed on artificial diet had a prolonged developmental time and increase in weight, and laid fewer eggs than those fed on natural diet. C. pumilio completed its development on the artificial diet and both the F, and F2 generations fed on an artificial diet were able to lay eggs. This could be the first step towards finding an artificial diet that would allow continuous rearing of C. pumilio and their availability at all times for utilization in the biological control of houseflies.
AFRIKAANSE OPSOMMING: In opnames wat op pluimveeplase in die Westelike Kaapprovinsie, Suid-Afrika uitgevoer is, is gevind dat die vliegsoorte Musca damestica L., Fannia canicularis (L.) en Leptacera sp. volop is. Die meeste van die M. damestica larwes en die myt Practalaelaps sp. is versamel in hoendermis met temperature tussen 20 en 26°C en die larwes van Ficanicularis en Leptacera tussen 14 en 22°C. Die volopste predatoriese arthropode in die mis was die histerid Carcinaps pumilia Erichson, die staphylinid Philanthus sardidus (Gravenhorst) en die myt Machrascheles muscaedamesticae (Scopoli). Hulle temperatuurtoleransie in die mis was tussen 12 en 31°C. Machrasceles muscaedamesticae en P. sardidus het vars mis verkies, terwyl C. pumilio voorkeur aan ouer mis gegee het. Gevolglik behoort hierdie twee predatore mekaar te komplementeer in programme van ge-integreerde plaagbestuur. In Studie van die vertikale deursnee-profiel van die miskeel het duidelik getoon dat die prooispesies M. damestica en en F canicularis en die predator P. sardidus in die kroon van die miskeel voorkom. Die opeenvolging van die arthropode in die ophopende hoendermis en die invloed van mishoogte op hulle getalle is bestudeer. Die prooispesies M damestica en F canicularis was die eerste koloniseerders, gevolg deur die predatore P. sardidus en M muscaedamesticae. Carcinaps pumilia was In laat koloniseerder. Die getalle van die larwes van M damestica en F. canicularis was negatief gekorreleer met die toename in mishoogte terwyl die getalle van die predartore C. pumilia en M. muscaedamesticae positief daarmee gekorreleer was. Die totale ontwikkelingstyd van C. pumilia van eier tot die volwassene se verskyning was 20.5 dae by 30°C. Twee larwale instars is gevind. Die onvolwasse stadia het 50% mortaliteit ondergaan voor die verskyning van die volwassenes. Die volwassenes van C. pumilia kan tot 130 dae lank by 30°C lewe en het In Tipe 1 oorlewingskurwe gehad. Die intrinsieke tempo van natuurlike toename (rm) was 0.064943 en die netto reproduksietempo (Rs) 20.191. Die tempo van eierlegging was in direkte verhouding tot die liggaamsgrootte en massa. Toename in digtheid het 'n onderdrukkende effek op vrugbaarheid gehad en tot 'n toename in ontwikkelingstyd gelei. Digtheid sal dus 'n kritieke faktor wees III emge program van massateling vir hierdie predator van vlieglarwes. Daarmee saam het 'n toename in digtheid die verspreiding van C. pumilio gestimuleer. By 'n digtheid van 50 C. pumilio volwassenes per houer het die verspreiding nie 2.5% oorskrei nie, in vergelyking met tot 24.0% by 'n dightheid van 400. Die predasietempo van vliee deur C. pumilio in die laboratorium het afgeneem met 'n toename in predatordigtheid, maar toegeneem met 'n toename in verhongering. Verhongering het nie 'n invloed gehad op die predasietempo van M. muscaedomesticae nie. 'n Tegniek met Drosophila melanogaster Meig. as bron van prooi in die teling van C pumilio is suksesvol ontwikkel en dit kan van kommersiele waarde wees. Dit blyk dat D. melanogaster ideaal kan wees as 'n bron van prooi in die massateling van Cpumilio vanwee sy kort ontwikkkelingstyd van ongeveer tien dae. Die spesies is ook goedkoop en maklik om te teel, en het 'n baie hoe biotiese potensiaal.Care inops pumilio wat op 'n kunsmatige dieet gevoed het, het 'n verlengde ontwikkelingstyd en gewigstoename gehad, en het minder eiers gele as die wat op 'n natuurlike dieet gevoed het. C. pumilio het sy lewensloop op die kunsmatige dieet voltooi en beide die FJ en F2 generasies wat op die kunsmatige dieet gevoed het, was in staat om eiers te Ie. Dit kan die eerste stap wees in 'n poging om 'n kunsmatige dieet te vind wat dit sal moontlik maak om C. pumilio aaneenlopend te teel sodat dit deurlopend beskikbaar kan wees vir gebruik in die biologiese beheer van huisvliee,

The effect of protein in adult and larval diets for Ophyra aenescens (Wiedemann) (Diptera: Muscidae ), a facultative predator of the house fly, Musca domestica L., was examined. A larval diet of coarse wheat bran, vermiculite and dried meat powder was found adequate for rearing O. aenescens larvae. Protein content was important for maximum larval emergence and for F1 adult longevity and fecundity. Adult diet of powdered milk, sugar and dried meat powder was sufficient for maintenance of adult O. aenescens flies. Protein was needed in adult diet for optimal fecundity. Cyromazine was toxic to O. aenescens at high levels (1.0 ppm), but at 0.75 ppm O. aenescens tolerated cyromazine better than a susceptible strain of house flies. At these cyromazine levels, mass release of O. aenescens into poultry houses is possible one to two days after cyromazine has been removed from the chicken feed. The effect of temperature and manure moisture on O. aenescens was studied. At temperatures of 18°C, emergence of Ophyra aenescens was significantly lower than at temperatures of 21 and 27°C. Predation by O. aenescens at 27°C on house fly larvae was significant at ratios of 3:1, 2:1 and 1:1 (house fly to O. aenescens). A constant manure moisture was important in the development of O. aenescens larvae and its ability to prey on house fly larvae. At constant levels of 50, 60, and 70% manure moisture, O. aenescens substantially reduced house fly larval numbers at ratios of 3:1, 2:1 and 1:1.
Master of Science

House flies (Musca domestica L.) are common pests affecting horses and their owners. Control of house flies in stable yards is currently based on the use of pesticides. However, the development of resistance by these flies to most pesticide groups has motivated horse owners to seek alternative methods of fly control. An entomopathogenic fungus, Beauveria bassiana (Bb) and an entomopathogenic bacterium, Bacillus thuringiensis var. israelensis (Bti) are two biological agents known to have activity against house flies. The broad objective of this study was to evaluate the effect of these two biological control agents on house flies in an equine environment. Using a structured questionnaire, presented in Chapter 2, thirty horse owners in KwaZulu-Natal were asked about the nuisance value of house flies, their current control measures, the potential market for biocontrol agents against house flies, and each owner’s perception of biocontrol methods. The horse owners were using three methods of house fly control namely, physical, chemical and biological. Most horse owners (97%) wanted access to effective biocontrol agents for control of house flies. Most horse owners (80%) stabled their horses at night, some or all of the time. The resultant manure piles in the stable yard were considered to be the primary cause of house fly problems. About 64% of the horse owners were dissatisfied with the currently available methods of controlling house flies in this situation. Chapter 3 covers two observational trials in which varying doses of Bacillus thuringiensis var. israelensis (Bti) were fed to horses, in order to identify a baseline dosage to give to horses in order to adequately control house fly populations growing in horse manure. The bacterium Bti, grown on wheat bran, was fed to six miniature horses at doses of 0, 0.125, 0.25, 0.5, 0.75, and 1.0 g per meal in Trial 3a, and at 0, 0.5, 1, 2, 4 and 8 g per meal in Trial 3b. Faeces were collected three times a week for 11 weeks and placed in incubation trays to allow the number of emerging adult house flies and closed pupae to be counted. In Trial 3a, there was a significant reduction in the number of closed pupae with an increase in Bti in the feed. The regression equation suggests that there will be 3.1 times as many closed pupae in the faeces when horses are fed 1 g of Bti in their feed, than when horses are fed no Bti. This dosage is the minimum baseline dosage for future trials. v Using manure from horses dosed in Trials 3a and 3b, the survival of the bacterium through the gut of horses was evaluated using a standard isolation technique. The growth of Bt colonies on the manure after the Bt isolation technique showed that some of the bacterial cells survived transition through the digestive tract of the horse. This study was qualitative in nature and did not attempt to quantify the level of Bti spore survival. These two observations suggest that Bacillus thuringiensis var. israelensis has the potential to be used as a biocontrol agent, applied via horse feed, for the control of house flies in stable yards. Future clinical trials, with appropriate replication, should be conducted using 1 g Bti/meal as the lowest test dosage. The objective of Chapter 4 was to determine whether spraying Bti or Bb on to horse manure is effective in the control of house flies. Over a six week period, two spraying trials were conducted in which increasing doses of Bb and Bti were sprayed on to 500 g samples of horse manure. Counts of house fly pupae and adults were taken. The doses of Bb and Bti tested were 0, 1, 2, 4 g in Trial 4a, and 0, 4, 8 and 12 g in Trial 4b. The research reported in Chapter 4 was characterized by the unexpectedly high levels of biological variation in egg, larvae and pupae numbers that were found in samples of horse manure, taken from the same skip two days apart. The statistical design of the two trials conducted was inadequate to cope with the high level of variation about treatment means for fly and larval counts. However, despite the lack of significant differences between treatment means, there is observational evidence that suggests that both Bb and Bti do have an effect on house fly survival. A simplified statistical model, which compared the number of hatched house flies on untreated manure, with the number on manure treated with any level of Bb (1 to 4 g /250 ml water), found a significant reduction in the number of hatched flies on treated manure. There was no significant corresponding reduction in the number of closed pupae, which suggests that Bb acts primarily before the larva pupates. The optimal dose of Bb and Bti to be sprayed on to manure could not be determined because of the high variation about treatment means. It is suggested that, in future trials similar dosages for Bb could be tested, but that higher dosages of Bti (starting at 2 g/250 ml water) should be used. Trial periods should be extended and replication increased dramatically to reduce variation about treatment means. Transformation of data before analysis may also be necessary to equalize variation about treatment means.
M.Sc.Agric. University of KwaZulu-Natal, Pietermaritzburg 2013.

The entomopathogenic fungus Beauveria bassiana and the bacterium Bacillus thuringiensis var. israelensis (Bti) have been widely studied for their role in biocontrol against many arthropods and extensively exploited for insect pest control. The purpose of this study was to evaluate the effect of four B. bassiana and two Bti formulations and their respective combinations, for the biological control of the common house fly, Musca domestica L., a major pest in poultry facilities. In vitro screening was undertaken to select the best B. bassiana isolates from 34 B. bassiana isolates and two Paecilomyces isolates. All the isolates of B. bassiana were found to be effective against adult house flies, but were marginally effective in controlling fly larvae. The Paecilomyces isolates were non-pathogenic towards both adult house flies and larvae. The best four isolates R444, 7320, 7569 and 7771 caused >90% mortality within 2d and were subjected to dose-mortality bioassays. Microscopic studies using light and scanning electron microscopy indicated the different durations of the lifecycle of B. bassiana development on the house fly. High temperature was found to delay conidial germination. Spore germination and mycelial growth were also inhibited by high adjuvant concentrations. Laboratory baseline bioassay data established, a dose-time response relationship using a waterdispersible granules (WDG) Bti formulation that demonstrated that the susceptibility of M. domestica larvae to a given concentration of Bti increased as the duration of exposure increased. In the laboratory studies, the LC50 and LC90 values of Bti for the larvae ranged between 65 - 77.4 and 185.1 - 225.9?g ml-1, respectively. LT50 and LT90 values were 5.5 and 10.3d respectively. In the field, a concentration of 10g Bti kg-1 (bran formulation) of feed resulted in 90% reduction of larvae for 4wk post-treatment. A higher concentration (2g L-1) of Bti in spray (WDG) applications was not significantly more effective than the lower concentration of 1g L-1. Thus, adding Bti to chicken feed has potential for the management and control of house flies in cagedpoultry facilities. The impact of oral feed applications of a bran formulation of Bti and a commercial chemical larvicide, Larvadex®, were compared with respect to their efficacy on the control of house fly 3 larval populations in poultry manure. The sublethal effects were manifested in terms of decreasing emergence of adult house flies. Although Larvadex® reduced larval density and caused significant reductions in emergence of adult house flies, it generally exhibited weaker lethal effects than Bti. The reduction levels achieved as a result of feeding 250mg Bti kg-1 at 5wk were similar to those achieved as a result of feeding twice the amount of Larvadex® at 4wk to the layers. From both an efficiency and economic perspective, comparisons to assess the impact of combining different concentrations of the two Bti formulations were carried out to evaluate their success in controlling house fly larvae and adults in poultry houses. The percentage mortality of larvae accomplished as a result of using a combination of 250mg kg-1 Bti in feed and 2g L-1 spray applications was equivalent to that obtained as a result of combining 500mg kg-1 Bti in feed and 1g L-1 spray application. The cost-benefit analysis (expressed in terms of mortality of larvae) indicated that the most effective combination for control of house fly larvae and fly emergence was the 500mg kg-1 in feed and 2g L-1 spray application combination that resulted in 67% larval mortality and 74% inhibition of adult house fly emergence. This study presents commercial users with possible combinations of applications of the two Bti formulations. Comparisons of larval mortalities and house fly emergence resulting from the Bti - B. bassiana treatments with those from Larvadex® - B. bassiana treatments, showed better control levels compared to any of the individual agents alone. The Bti treatments were more effective at controlling larval populations and inhibiting the emergence of house flies than Larvadex®, even when Larvadex® was applied together with B. bassiana. The effects of the Bti - B. bassiana and the Larvadex® - B. bassiana interactions were additive. These trials suggest that the efficacy of Bti in the control of house fly larvae may be improved with frequent applications of B. bassiana.
Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2008.