Academic literature on the topic 'Housekeeping expression'

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Journal articles on the topic "Housekeeping expression"

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Barber, Robert D., Dan W. Harmer, Robert A. Coleman, and Brian J. Clark. "GAPDH as a housekeeping gene: analysis of GAPDH mRNA expression in a panel of 72 human tissues." Physiological Genomics 21, no. 3 (2005): 389–95. http://dx.doi.org/10.1152/physiolgenomics.00025.2005.

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Quantitative gene expression data are often normalized to the expression levels of control or so-called “housekeeping” genes. An inherent assumption in the use of housekeeping genes is that expression of the genes remains constant in the cells or tissues under investigation. Although exceptions to this assumption are well documented, housekeeping genes are of value in fully characterized systems. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is one of the most commonly used housekeeping genes used in comparisons of gene expression data. To investigate the value of GAPDH as a housekeeping ge
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Hanafy, Sherif, and Fakhreddin Jamali. "Adjuvant arthritis influences expression of housekeeping genes." Inflammation Research 60, no. 6 (2011): 521–23. http://dx.doi.org/10.1007/s00011-011-0327-4.

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Mondello, Chiara, and Peter N. Goodfellow. "Methylation and expression of a housekeeping gene." Trends in Genetics 1 (January 1985): 124–25. http://dx.doi.org/10.1016/0168-9525(85)90047-2.

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Chen, Ren, Mayumi Gyokusen, Yoshihisa Nakazawa, and Koichiro Gyokusen. "Selection of Housekeeping Genes for Transgene Expression Analysis in Eucommia ulmoides Oliver Using Real-Time RT-PCR." Journal of Botany 2010 (March 17, 2010): 1–7. http://dx.doi.org/10.1155/2010/230961.

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In order to select appropriate housekeeping genes for accurate calibration of experimental variations in real-time (RT-) PCR results in transgene expression analysis, particularly with respect to the influence of transgene on stability of endogenous housekeeping gene expression in transgenic plants, we outline a reliable strategy to identify the optimal housekeeping genes from a set of candidates by combining statistical analyses of their (RT-) PCR amplification efficiency, gene expression stability, and transgene influences. We used the strategy to select two genes, ACTα and EF1α, from 10 can
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Rekawiecki, Robert, Magdalena Kowalik, and Jan Kotwica. "Validation of housekeeping genes for studying differential gene expression in the bovine myometrium." Acta Veterinaria Hungarica 61, no. 4 (2013): 505–16. http://dx.doi.org/10.1556/avet.2013.037.

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The aim of this study was to determine the steady-state expression of 13 selected housekeeping genes in the myometrium of cyclic and pregnant cows. Cells taken from bovine myometrium on days 1–5, 6–10, 11–16 and 17–20 of the oestrous cycle and in weeks 3–5, 6–8 and 9–12 of pregnancy were used. Reverse transcribed RNA was amplified in real-time PCR using designed primers. Reaction efficiency was determined with the Linreg programme. The geNorm and NormFinder programmes were used to select the best housekeeping genes. They calculate the expression stability factor for each used housekeeping gene
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Cohen-Tannoudji, Michel, Sandrine Vandormael-Pournin, Jean-Michel Drezen, Pascale Mercier, Charles Babinet, and Dominique Morello. "lacZ sequences prevent regulated expression of housekeeping genes." Mechanisms of Development 90, no. 1 (2000): 29–39. http://dx.doi.org/10.1016/s0925-4773(99)00226-9.

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He, Bing, Hui Chen, Pibiao Shi, et al. "Systematic Identification and Validation of Housekeeping and Tissue-Specific Genes in Allotetraploid Chenopodium quinoa." Horticulturae 7, no. 8 (2021): 235. http://dx.doi.org/10.3390/horticulturae7080235.

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Quinoa is a gluten-free food crop that contains all the essential amino acids and vitamins. The selection of proper housekeeping and tissue-specific genes is the crucial prerequisite for gene expression analysis using the common approach, real-time quantitative PCR (RT-qPCR). In this study, we identified 40 novel candidate housekeeping genes by the minimum transcript per million (TPM), coefficient of variation (CV) and maximum fold change (MFC) methods and 19 candidate tissue-specific genes by the co-expression network method based on an RNA-seq dataset that included 53 stem, leaf, flower and
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Meller, M., S. Vadachkoria, D. A. Luthy, and M. A. Williams. "Evaluation of housekeeping genes in placental comparative expression studies." Placenta 26, no. 8-9 (2005): 601–7. http://dx.doi.org/10.1016/j.placenta.2004.09.009.

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Chambers, R. C. "Gene expression profiling: good housekeeping and a clean message." Thorax 57, no. 9 (2002): 754–56. http://dx.doi.org/10.1136/thorax.57.9.754.

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Roslim, Dewi Indriyani, Liza Aulia Yusfi, Desriani Ritawati Hutagalung, et al. "Isolation of Housekeeping Genes on Durik-durik (Syzygium sp)." Biosaintifika: Journal of Biology & Biology Education 10, no. 2 (2018): 237–44. http://dx.doi.org/10.15294/biosaintifika.v10i2.14234.

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Housekeeping gene is a gene expressed with a fixed level and in abundant amounts under various conditions. After validation, the housekeeping gene can be used as an internal control to normalize gene expression data. This study reports the isolation of several housekeeping genes in Durik-durik plant (Syzygium sp). This plant material in form of fresh leaves from Durik-durik plants are taken from Kajuik Lake, Riau Province. The next stage is total DNA isolation, polymerase chain reaction, electrophoresis, sequencing and data analysis using bioinformatic tools. The isolated housekeeping genes in
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Dissertations / Theses on the topic "Housekeeping expression"

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Tan, Wooi-keng, and 陳慧卿. "Expression patterns of housekeeping genes in cancer." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2004. http://hub.hku.hk/bib/B29892892.

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Rettschlag, Jeannine. "Linksventrikuläre Expression verschiedener Housekeeping-Gene bei kardialer Hypertrophie und Herzinsuffizienz." [S.l.] : [s.n.], 2003. http://deposit.ddb.de/cgi-bin/dokserv?idn=970211457.

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Rettschlag, Jeannine. "Linksventrikuläre Expression verschiedener Housekeeping-Gene bei kardialer Hypertrophie und Herzinsuffizienz." Doctoral thesis, Humboldt-Universität zu Berlin, Medizinische Fakultät - Universitätsklinikum Charité, 2003. http://dx.doi.org/10.18452/15002.

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Das Ziel dieser Arbeit war es einen geeigneten internen Standard für die linksventrikuläre mRNA-Quantifizierung bei kardialer Hypertrophie und Herzinsuffizienz in der Ratte zu finden. Die mRNA-Expression von GAPDH, 18SrRNA, Cyclophilin and Porphobilinogen-Desaminase (PBGD) wurde vier Wochen nach Induktion von Hypertrophie (kleiner aortokavaler Shunt) und Herzinsuffizienz (großer aortokavaler Shunt bzw. Myokardinfarkt) mit Hilfe des Ribonuklease Protektion Assay (RPA) und der TaqMan PCR bestimmt. Die linksventrikuläre ANP-mRNA-Expression war in allen untersuchten Modellen unabhängig von der an
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Al-Hosni, Aliya M. R. "Roles for the VEZF1 transcription factor in erythroid and housekeeping gene expression." Thesis, University of Glasgow, 2017. http://theses.gla.ac.uk/8118/.

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VEZF1 is a DNA-binding transcription factor that is highly conserved in vertebrates. Human VEZF1 has recently been found to interact with transcriptionally active gene promoters and erythroid specific enhancers. It is unclear how the broadly expressed VEZF1 interacts with enhancers in a cell type-specific manner or what functions it plays at these elements. This work begins to address the cell-type specific roles of VEZF1 by creating loss of function models in a human erythroid cell line.
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MATOS, Mitalle Karen da Silva. "Expressão diferencial e diversidade de fatores de transcrição da família MYB em Feijão-Caupi." Universidade Federal de Pernambuco, 2015. https://repositorio.ufpe.br/handle/123456789/17336.

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Submitted by Fabio Sobreira Campos da Costa (fabio.sobreira@ufpe.br) on 2016-07-12T13:09:04Z No. of bitstreams: 2 license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) Dissertacao_MitalleMatos_PPGG.pdf: 3448234 bytes, checksum: fe598459348fe967a8f1c2e5067f7d99 (MD5)<br>Made available in DSpace on 2016-07-12T13:09:04Z (GMT). No. of bitstreams: 2 license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) Dissertacao_MitalleMatos_PPGG.pdf: 3448234 bytes, checksum: fe598459348fe967a8f1c2e5067f7d99 (MD5) Previous issue date: 2015-03-02<br>CNPq<br>Danos cau
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Rettschlag, Jeannine [Verfasser], P. [Gutachter] Persson, R. H. G. [Gutachter] Schwinger, and Roland [Gutachter] Willenbrock. "Linksventrikuläre Expression verschiedener Housekeeping-Gene bei kardialer Hypertrophie und Herzinsuffizienz / Jeannine Rettschlag ; Gutachter: P. Persson, R. H. G. Schwinger, Roland Willenbrock." Berlin : Humboldt-Universität zu Berlin, 2003. http://d-nb.info/1207665835/34.

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Åberg, Anna. "New insights into the role of ppGpp and DksA through their effect on transcriptional regulation of housekeeping and colonization related genes of Escherichia coli /." Umeå : Univ, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-1669.

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Carkacioglu, Levent. "Automated Biological Data Acquisition And Integration Using Machine Learning Techniques." Phd thesis, METU, 2009. http://etd.lib.metu.edu.tr/upload/12610396/index.pdf.

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Since the initial genome sequencing projects along with the recent advances on technology, molecular biology and large scale transcriptome analysis result in data accumulation at a large scale. These data have been provided in different platforms and come from different laboratories therefore, there is a need for compilation and comprehensive analysis. In this thesis, we addressed the automatization of biological data acquisition and integration from these non-uniform data using machine learning techniques. We focused on two different mining studies in the scope of this thesis. In the first st
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Corrales, Berjano Marc 1984. "Text in context : Chromatin effects in gene regulation." Doctoral thesis, Universitat Pompeu Fabra, 2018. http://hdl.handle.net/10803/565719.

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This thesis reports the study of chromatin composition and conformation on the expression of integrated reporters at thousands of genomic locations in the Drosophila genome. We have adapted and improved a technology (Thousands of Reporters Integrated in Parallel TRIP) to randomly integrate barcoded reporters allowing us to measure the context effects on transcription at ~80.000 different loci. We have focused on housekeeping promoter-reporters due to their relative autonomy from distal regulatory elements. Taking advantage of published genome-wide localization maps of chromatin protein and hi
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Mehtali, Abdel-Majid. "Les genes de maintenance : etude du gene hmgcoa reductase in vitro et dans les souris transgeniques." Université Louis Pasteur (Strasbourg) (1971-2008), 1988. http://www.theses.fr/1988STR13162.

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Les genes de maintenance sont exprimes de maniere ubiquitaire par un mecanisme encore incompris. Pour aborder ce probleme, les auteurs ont choisi comme modele d'etude un gene de maintenance typique, le gene de l'hydroxymethylglutaryl coa reductase (hmgcr), enzyme cle de la biosynthese du cholesterol. Le gene hmgcr de souris a ete isole et sa region promotrice caracterisee; l'expression et le profil de methylation de plusieurs genes chimeriques, dans lesquels le gene marqueur cat est sous le controle de sequences promotrices hmgcr de taille decroissante, ont ete etudies in vitro (par transforma
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Book chapters on the topic "Housekeeping expression"

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Li, Li, Xiaofang Mao, Qiang Gao, and Yicheng Cao. "Sample-Independent Expression Stability Analysis of Human Housekeeping Genes Using the GeNORM Algorithm." In Lecture Notes in Electrical Engineering. Springer Netherlands, 2013. http://dx.doi.org/10.1007/978-94-007-7618-0_8.

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Bonham-Carter, Oliver, and Yee Mon Thu. "GenExSt: A Tool to Identify Correlation of Gene Expression After Normalization with Housekeeping Genes." In Advances in Intelligent Systems and Computing. Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-73103-8_5.

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Fraiman, Susan. "Bad Girls of Good Housekeeping." In Extreme Domesticity. Columbia University Press, 2017. http://dx.doi.org/10.7312/columbia/9780231166348.003.0005.

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Proposes a “bad girl” tradition going back to the nineteenth century in which domestic zeal and expertise are linked to women who resist compulsory heterosexuality; who are childless, child-averse, or single mothers; whose domesticity does not preclude and may actually foster professionalism; whose homemaking is divorced from selfless marital/maternal duty and expressive instead of female self-sufficiency, ambition, and pleasure. Begins with a handful of early figures before turning to Dominique Browning’s Around the House and in the Garden: A Memoir of Heartbreak, Healing, and Home Improvement (2003) for a rich account of post-marital nesting. Concludes by touting Martha Stewart’s lifestyle empire as a brazen example of greedy and grandiose domesticity.
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Conference papers on the topic "Housekeeping expression"

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Peters, Tracy L., Elizabeth J. Ferree, Yaou Sheng, and Aaron E. Hoffman. "Abstract 3403: Circadian fluctuations in “housekeeping” gene expression measured by RNA-seq." In Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CA. American Association for Cancer Research, 2014. http://dx.doi.org/10.1158/1538-7445.am2014-3403.

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Wang, Albert R., Gopal Iyer, Sean Brennan, et al. "Abstract 827: Statistical analyses of stable housekeeping gene expression in cancer post-irradiation." In Proceedings: AACR Annual Meeting 2017; April 1-5, 2017; Washington, DC. American Association for Cancer Research, 2017. http://dx.doi.org/10.1158/1538-7445.am2017-827.

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Shah, Khyati Niral, and Jesika S. Faridi. "Abstract 2920: Estrogen, tamoxifen, and Akt modulate expression of putative housekeeping genes in breast cancer cells." In Proceedings: AACR 102nd Annual Meeting 2011‐‐ Apr 2‐6, 2011; Orlando, FL. American Association for Cancer Research, 2011. http://dx.doi.org/10.1158/1538-7445.am2011-2920.

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Shikata, Tetsuo, Toshihiko Shiraishi, Kumiko Tanaka, Shin Morishita, and Ryohei Takeuchi. "Effects of Acceleration Amplitude and Frequency of Mechanical Vibration on Osteoblast-Like Cells." In ASME 2007 International Mechanical Engineering Congress and Exposition. ASMEDC, 2007. http://dx.doi.org/10.1115/imece2007-41797.

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Bone formation is subject in vivo to mechanical stimulation. Although many researches for bone cells of osteoblastic lineage sensing and responding to mechanical stimulation have been reported mainly in the biochemical field, effects of mechanical stimulation on bone cells are not well understood. In this study, in order to clarify effects of acceleration amplitude and frequency of mechanical stimulation on MC3T3-E1, which is an osteoblast-like cell line derived from mouse calvaria, in the sense of mechanical vibrations, their cell proliferation, cell morphology, bone matrix generation and gen
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Causin, R. Lima, KCristina Borba de Souza, L. Ferro Leal, A. Feijó Evangelista, and G. Macedo Matsushita. "PO-393 Identification of housekeeping genes to quantitative real-time RT-PCR analysis by miRNA expression using liquid-based cervical cytologysamples." In Abstracts of the 25th Biennial Congress of the European Association for Cancer Research, Amsterdam, The Netherlands, 30 June – 3 July 2018. BMJ Publishing Group Ltd, 2018. http://dx.doi.org/10.1136/esmoopen-2018-eacr25.420.

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Shikata, Tetsuo, Toshihiko Shiraishi, Kumiko Tanaka, Shin Morishita, and Ryohei Takeuchi. "Effects of Amplitude and Frequency of Vibration Stimulation on Cultured Osteoblasts." In ASME 2007 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. ASMEDC, 2007. http://dx.doi.org/10.1115/detc2007-34949.

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Mechanical stimulation to bones affects osteogenesis such as decrease of bone mass of astronauts under zero gravity, walking rehabilitation to bone fracture and fracture repair with ultrasound devices. Bone cells have been reported to sense and response to mechanical stimulation at cellular level morphologically and metabolically. In the view of mechanical vibrations, bone cells are deformed according to mechanical stimulation and their mechanical characteristics. Recently, it was reported that viscoelasticity of cells was measured using tensile and creep tests and that there was likely natura
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Shikata, Tetsuo, Toshihiko Shiraishi, Shin Morishita, and Ryohei Takeuchi. "Effects of Acceleration Amplitude and Frequency of Mechanical Vibration on Cultured Osteoblasts." In ASME 2008 International Mechanical Engineering Congress and Exposition. ASMEDC, 2008. http://dx.doi.org/10.1115/imece2008-67221.

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This paper describes the effects of the frequency and acceleration amplitude of mechanical vibration on osteoblasts, the bone cells that generate the bone matrix. Their cell proliferation and bone matrix generation were investigated when sinusoidal inertia force was applied to the cells. Bone formation is subject in vivo to mechanical stimulation. Although many researches for bone cells of osteoblastic lineage sensing and responding to mechanical stimulation have been reported mainly in the biochemical field, effects of mechanical stimulation on bone cells are not well understood. After the ce
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Fleming, Paul, and Tara Dalton. "One-Step Reverse-Transcription PCR on a High-Throughput Micro-Fluidic Device." In ASME 2009 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2009. http://dx.doi.org/10.1115/sbc2009-206623.

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One step reverse-transcription polymerase chain reaction (RT-PCR) assays are an attractive option for further automating gene detection assays. One-step assays can reduce hands–on-time and the risk of sample crossover and contamination. The one-step chemistries are showing increasing use in virus detection and have been reported, in some cases, to be more appropriate than their two-step counterparts [1, 2]. Previous work presented by the Stokes Institute research group outlined a micro fluidic based continuous flow instrument which performed high throughput qPCR in nanolitre sized droplets [3]
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