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Journal articles on the topic 'HPLC.DAD (High Performance Liquid Chromatograph-Diode Array Detector)'

Author: Grafiati

Published: 10 September 2021

Last updated: 29 July 2025

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1

Tuzimski, Tomasz. "Determination of Pesticides in Water Samples from the Wieprz-Krzna Canal in the czysko-Wodawskie Lake District of Southeastern Poland by Thin-Layer Chromatography with Diode Array Scanning and High-Performance Column Liquid Chromatography with Diode Array Detection." Journal of AOAC INTERNATIONAL 91, no. 5 (2008): 1203–9. http://dx.doi.org/10.1093/jaoac/91.5.1203.

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Abstract High-performance thin-layer chromatography with diode array scanning (TLC-DAD) and high-performance column liquid chromatography with a diode array detector (HPLC-DAD) were used to screen water samples for pesticides. Atrazine, clofentezine, chlorfenvinphos, hexaflumuron, terbuthylazine, lenacyl, neburon, bitertanol, and metamitron were enriched from canal water samples by solid-phase extraction (SPE) on octadecyl silane (C18)/styrene-divinylbenzene-1, C18, C18 Polar Plus, and cyanopropyl (CN) cartridges. Recovery rates were high for all extraction materials except CN, for which value

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Cebrián-Tarancón, Cristina, José Oliva, Miguel Ángel Cámara, Gonzalo L. Alonso, and M. Rosario Salinas. "Analysis of Intact Glycosidic Aroma Precursors in Grapes by High-Performance Liquid Chromatography with a Diode Array Detector." Foods 10, no. 1 (2021): 191. http://dx.doi.org/10.3390/foods10010191.

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Nowadays, the techniques for the analysis of glycosidic precursors in grapes involve changes in the glycoside structure or it is necessary the use of very expensive analytical techniques. In this study, we describe for the first time an approach to analyse intact glycosidic aroma precursors in grapes by high-performance liquid chromatography with a diode array detector (HPLC-DAD), a simple and cheap analytical technique that could be used in wineries. Briefly, the skin of Muscat of Alexandria grapes was extracted using a microwave and purified using solid-phase extraction combining Oasis MCX a

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3

Yang, Fangliang, Guoxiang Sun, and Jiao Chen. "Development of a HPLC-DAD method combined with multicomponent chemometrics and antioxidant capacity to monitor the quality consistency of compound bismuth aluminate tablets by comprehensive quantified fingerprint method." Analytical Methods 9, no. 27 (2017): 4082–90. http://dx.doi.org/10.1039/c7ay00916j.

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A high performance liquid chromatography (HPLC) coupled with diode array detector method was developed to monitor the quality consistency of a classical compound preparation, compound bismuth aluminate tablet.

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4

Seo, Chang-Seob, and Kwang-Hoon Song. "Phytochemical Characterization for Quality Control of Phyllostachys pubescens Leaves Using High-Performance Liquid Chromatography Coupled with Diode Array Detector and Tandem Mass Detector." Plants 11, no. 1 (2021): 50. http://dx.doi.org/10.3390/plants11010050.

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Phyllostachys pubescens leaves are cultivated in a number of Asian countries and have been used for antipyretic and diuretic effects since ancient times, especially in Korea. The purpose of this study was to develop and validate of analytical method for quality control of P. pubescens leaves using high-performance liquid chromatography with diode array detector (HPLC–DAD) and liquid chromatography with tandem mass spectrometry (LC–MS/MS) detection. HPLC–DAD analysis was conducted with a Gemini C18 column, and distilled water–acetonitrile (both with 0.1% (v/v) formic acid) mobile-phase system.

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Wang, Hong Jun, Tie Zhong Zhou, Hong Jiang, et al. "The Establishment of the Fingerprint of Bangduling Oral Liquid by the High Performance Liquid Chromatography-Diode Array Detector." Advanced Materials Research 694-697 (May 2013): 1047–50. http://dx.doi.org/10.4028/www.scientific.net/amr.694-697.1047.

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Bangduling (BDL) oral liquid, which is a new research and development herbal preparation, comprises four traditional Chinese medicines (TCM): Radix Scutellariae, Cortex Phellodendri Chinensis, Folium Isatidis, and Radix et Rhizoma Rhei. A simple, reliable and reproducible method, based on a high performance liquid chromatography-diode array detector (HPLC-DAD) method, for establishing fingerprint of complex herbal medicine BDL oral liquid was described firstly. In order to develop and validate this HPLC-DAD method, Kromasil C18 column (S-5μm, 4.6 mm × 250 mm) was used with gradient mobile phas

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Narzullo, Saidov, Smelova Natalia, Garna Natalya, Koretnik Oksana, Khanina Nataliia та Gubar Svetlana. "Development of HPLC method for quantitative determination of new perspective APhI with anti-ulcer activity of trіаzоprazol". ScienceRise: Pharmaceutical Science, № 6(16) (28 грудня 2018): 41–46. https://doi.org/10.15587/2519-4852.2018.153387.

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<strong>Aim.</strong> Development of optimal, high-precision, reproducible method of quantitative determination of triazoprazole in substance using the method of high performance liquid chromatography. <strong>Materials and methods.</strong> Physico-chemical (high-performance liquid chromatography) and mathematical (statistical processing of results) methods of research were used to achieve this goal. Chromatography was performed on a liquid chromatograph Agilent 1290 Infinity II with diode-array (LC 1290) detector and quadrupole-time-of-flight (QTOF 6530) mass analyzer. Fixed p

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Zhu, Yanqin, Qinhong Yin, and Yaling Yang. "A comparative study of HPLC-DAD and UPLC-UV methods for simultaneous determination of 11 polyphenols in Moringa oleifera leaves." Tropical Journal of Pharmaceutical Research 20, no. 11 (2021): 2371–79. http://dx.doi.org/10.4314/tjpr.v20i11.20.

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Purpose: To develop, validate and compare two chromatographic methods - high performance liquid chromatography with diode array detector ((HPLC-DAD) and high performance liquid chromatography with ultraviolet detection (UPLC-UV) for the effective analysis of polyphenols in Moringa oleifera leaves.Methods: HPLC-DAD and UPLC-UV methods were applied for the accurate determination of eleven major polyphenols in Moringa oleifera leaves. The chromatographic conditions of the eleven polyphenols was determined on two C18 column by gradient elution with 0.5 % phosphoric acid solution -acetonitrile as t

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Bittová, Miroslava, Dita Hladůvková, Vendula Roblová, Stanislav Kráčmar, Petr Kubáň, and Vlastimil Kubáň. "Analysis of Organic Acids, Deacetyl Asperulosidic Acid and Polyphenolic Compounds as a Potential Tool for Characterization of Noni (Morinda citrifolia) Products." Natural Product Communications 10, no. 11 (2015): 1934578X1501001. http://dx.doi.org/10.1177/1934578x1501001107.

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Organic acids, deacetyl asperulosidic acid (DAA) and polyphenolic compounds in various noni ( Morinda citrifolia L.) products (4 juices, 4 dry fruit powders and 2 capsules with dry fruit powder) were analyzed. Reversed-phase high-performance liquid chromatography (RP-HPLC) coupled with a variable wavelength detector (VWD) and electrospray ionization time-of-flight mass spectrometer (ESI-TOF MS) was applied for simultaneous analysis of organic acids (malic, lactic, citric and succinic acid) and DAA. An RP-HPLC method with diode-array detector (DAD) was developed for the analysis of polyphenolic

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9

Szewczyńska, Małgorzata, and Małgorzata Pośniak. "Doxorubicin hydrochloride. Determination in workplace air with high performance liquid chromatography." Podstawy i Metody Oceny Środowiska Pracy 34, no. 2(96) (2018): 115–31. http://dx.doi.org/10.5604/01.3001.0012.1025.

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This article presents a method for measuring doxorubicin hydrochloride in workplace air with HPLC with diode array detector (DAD). The method is based on adsorption inhalable fraction of doxorubicin hydrochloride aerosol on glass fiber filter, desperation with water, and chromatographic analysis. The analysis was performed in reverse phase (mobile phase – 0.05 mol/L hydrophosphate disodum and acetonitrile (65:35) with pH – 3 with 0.5 mL/L triethylamine) on C18 column. The measurement range was 0.06 – 1 μg/m3 for 4800-L air sample. The limit of detection (LOD) was 0,0005 μg/ml and the limit of

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Riley-Saldaña, Christian Anabí, María del Rocío Cruz-Ortega, Mariano Martínez Vázquez, Iván De-la-Cruz-Chacón, Marisol Castro-Moreno, and Alma Rosa González-Esquinca. "Acetogenins and alkaloids during the initial development of Annona muricata L. (Annonaceae)." Zeitschrift für Naturforschung C 72, no. 11-12 (2017): 497–506. http://dx.doi.org/10.1515/znc-2017-0060.

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AbstractIn plants, the presence and distribution of specialized metabolites during the early stages of development are not documented enough, even though their biosynthesis is one of the most important strategies for survival. In this study, five alkaloids and four acetogenins were detected inAnnona muricataL. during early development seedling, including three phases of root emergence and three of seedling formation. Hexane and alkaloid extracts were obtained from each organ, which were analyzed in a gas-mass chromatograph and in a high-performance liquid chromatograph coupled with a photodiod

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Alvarenga, Tavane Aparecida, Osvaine Júnior Alvarenga Alves, Mariana Cintra Pagotti, et al. "In vitro antileishmanial activity of Anacardium othonianum and isolated compounds against Leishmania amazonensis." Acta Brasiliensis 5, no. 1 (2021): 44. http://dx.doi.org/10.22571/2526-4338429.

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This study analyzes the antileishmanial activity of the crude ethanol extract, fractions, and isolated compounds of A. othonianum nuts. Antileishmanial activity was evaluated against Leishmania amazonensis promastigotes in vitro. The phytochemical study was performed by high-performance liquid chromatography-high-resolution mass spectrometry-diode array detector (HPLC-HRMS-DAD) and by preparative HPLC. HPLC-HRMS-DAD analysis of the bioactive extract confirmed the presence of ten alkyl phenol derivatives that had previously been isolated from A. occidentale. Bioassay-guided isolation afforded c

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Ragab, Marwa A. A., Mohamed H. Abdel-Hay, Hytham M. Ahmed, and Sara M. Mohyeldin. "Determination of Ibuprofen and Phenylephrine in Tablets by High-Performance Thin Layer Chromatography and in Plasma by High-Performance Liquid Chromatography with Diode Array Detection." Journal of Chromatographic Science 57, no. 7 (2019): 592–99. http://dx.doi.org/10.1093/chromsci/bmz031.

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Abstract Two chromatographic methods (high performance thin layer chromatography (HPTLC) and high performance liquid chromatography–diode array detector (HPLC-DAD)), were addressed for the analysis of a mixture consisted of phenylephrine hydrochloride and ibuprofen in two forms bulk and their combined dosage form. This binary mixture is considered to be a challenging one as the two drugs differ greatly in their chemical and physical properties. Not only this affects their simultaneous analysis, but also hinders their simultaneous extraction from biological fluids as plasma. That is the reason

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Tuzimski, Tomasz, and Tomasz Rejczak. "Determination of Pesticides in Sunflower Seeds by High-Performance Liquid Chromatography Coupled with a Diode Array Detector." Journal of AOAC INTERNATIONAL 97, no. 4 (2014): 1012–20. http://dx.doi.org/10.5740/jaoacint.sgetuzimski.

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Abstract The application of RP-HPLC with a diode array detector for identification and quantitative analysis of pesticides in sunflower seed samples is demonstrated. An HPLC procedure on C18 RP column has been developed for analysis of selected pesticides from different chemical groups: simazine, isoproturon, terbuthylazine, linuron, captan, terbutryn, procymidone, fenitrothion, clofentezine, and bromopropylate. We investigated the possibility of expanding the scope of the four analyte extraction procedures for isolation of pesticides from plant matrixes with high levels of lipids. The followi

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Loncar, Eva, Petar Sekulic, Tijana Zeremski-Skoric, Radomir Malbasa, and Ljiljana Kolarov. "Determination of phenmedipham and desmedipham in a commercial herbicide by high performance liquid chromatography." Acta Periodica Technologica, no. 35 (2004): 193–98. http://dx.doi.org/10.2298/apt0435193l.

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Betanal AM-11 is the herbicide that is using to control one-year old weeds with wide leafs in sugar beet fields. Active ingredients of the herbicide are phenmedipham and desmedipham. Commercial emulsifiable concentrate (EC) contains 80 g/L ( 10% of each active compound. We applied high performance liquid chromatography (HPLC) with diode array detector (DAD) at 254 nm for the determination of phenmedipham and desmedipham in commercial samples of Betanal AM-11. This method involves reversed-phase separation of the components on C-18 bonded silica with methanol-water (51+49, v/v) as the eluent. T

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Bekele, Habtamu, Abi Legesse, Weldegebriel Yohannes, and Negussie Megersa. "Development of cost-effective dispersive liquid-liquid micro-extraction technique for preconcentration of multi-residue herbicides in environmental waters prior to Chromatographic analysis." Bulletin of the Chemical Society of Ethiopia 38, no. 5 (2024): 1189–204. http://dx.doi.org/10.4314/bcse.v38i5.1.

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High-density dispersive liquid-liquid microextraction method coupled with high-performance liquid chromatography with a diode array detector (HD-DLLME-HPLC-DAD) was developed for extraction and determination of six commonly used sulfonylurea herbicides in matrices of environmental waters. For simultaneous extraction of the target herbicides, the optimum experimental parameters that 024influence extraction efficiency were investigated. Under optimized conditions, the limit of detection (LOD) and quantification (LOQ) were 0.8-1.5 and 1.9-5.1 ng mL−1, respectively. The precisions in terms of rela

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16

Nguyen Thanh, Dat, Hong Hanh Nguyen Thi, Thu Dam Thi, Hieu Nguyen Van, Phuong Lai Thi, and Bac Duong The. "Determination of adenosine and cordycepin in dietary supplements circulating on the Hanoi market." Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam 5, no. 3 (2022): 390–401. http://dx.doi.org/10.47866/2615-9252/vjfc.3958.

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High performance liquid chromatography with diode-array detector (HPLC-DAD) has&nbsp;been fully validated according to AOAC requirements for the determination of adenosine&nbsp;and cordycepin in dietary supplements containing Cordyceps. Chromatographic conditions&nbsp;using InertSustain C18 (250 mm &times; 4.6 mm; 5 &micro;m) column, with gradient program composed&nbsp;of methanol and water eluted in 35 min, diode-array detector at 260 nm, flow rate of 1.0&nbsp;mL/min. The study has determined the content of adenosine and cordycepin in 24 dietary&nbsp;supplement

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Szopa, Agnieszka, Michał Dziurka, Sebastian Granica, et al. "Schisandra rubriflora Plant Material and In Vitro Microshoot Cultures as Rich Sources of Natural Phenolic Antioxidants." Antioxidants 9, no. 6 (2020): 488. http://dx.doi.org/10.3390/antiox9060488.

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Schisandra rubriflora is a dioecious, underestimated medicinal plant species known from traditional Chinese medicine. The present study was aimed at characterising the polyphenolic profile composition and the related antioxidant capacity of S. rubriflora fruit, stem and leaf and in vitro microshoot culture extracts. Separate analyses of material from female and male specimens were carried out. This study was specifically aimed at detailed characterisation of the contribution of phenolic compounds to overall antioxidant activity using ultra-high-performance liquid chromatography with a photodio

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Serdar, Maja, and Zorka Knežević. "Determination of Artificial Sweeteners in Beverages and Special Nutritional Products Using High Performance Liquid Chromatography." Archives of Industrial Hygiene and Toxicology 62, no. 2 (2011): 169–73. http://dx.doi.org/10.2478/10004-1254-62-2011-2084.

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Determination of Artificial Sweeteners in Beverages and Special Nutritional Products Using High Performance Liquid ChromatographyThis paper presents two high performance liquid chromatographic (HPLC) methods used for the separation and determination of artificial sweeteners aspartame, acesulphame K, sodium saccharin, and sodium cyclamate in beverages and special nutritional products (special food intended for specific population groups). All four compounds are soluble in aqueous solutions and can easily be separated and determined by HPLC with a diode array detector (DAD). The first method inv

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Sobolev, R. V., I. E. Sokolov, N. A. Petrov, V. А. Sarkisyan, and A. A. Kochetkova. "Methods of extraction, separation and identification of cyclic peptides from flaxseed (Linum usitatissimum L.): A review." Food systems 7, no. 4 (2025): 535–42. https://doi.org/10.21323/2618-9771-2024-7-4-535-542.

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Oilseed flax (Linum usitatissimum L.) is a valuable crop characterized by a high content of fats, dietary fiber, protein and various biologically active substances, in particular cyclopeptides. Cyclic peptides are a group of cyclic hydrophobic peptides consisting of eight to ten amino acids with a molecular weight in the range of 950–2300 Da. Flax oil and seeds contain from 0.1 to 0.3% cyclopeptides, which can exhibit antioxidant, anti-inflammatory, immunosuppressive, antihypertensive and antitumor activity. The aim of this review was to systematize and summarize the available literature data

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Cinkmanis, Ingmārs, Ingrīda Augšpole, Irina Sivicka, and Sanita Vucāne. "Evaluation of the Phenolic Profile of Bear’s Garlic (Allium Ursinum L.) Leaves." Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences. 76, no. 4 (2022): 512–16. http://dx.doi.org/10.2478/prolas-2022-0079.

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Abstract European wild garlic (Allium ursinum L.) syn. wood garlic, ramsons or bear’s garlic is a perennial plant that is common in Europe and Asia, but rare in the Mediterranean region. Bear’s garlic is included in the Latvian Red Data Book. The plant species has high commercial value and a high amount of biologically active compounds. Because of the conservation status of wild populations, raw material should be obtained by cultivation. For the experiment, leaves of six accessions of bear’s garlic from the ex situ collection of medicinal and aromatic plants at the Latvia University of Life S

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Orfanoudaki, Maria, Anja Hartmann, Julia Mayr, et al. "Analysis of the Mycosporine-Like Amino Acid (MAA) Pattern of the Salt Marsh Red Alga Bostrychia scorpioides." Marine Drugs 19, no. 6 (2021): 321. http://dx.doi.org/10.3390/md19060321.

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This study presents the validation of a high-performance liquid chromatography diode array detector (HPLC-DAD) method for the determination of different mycosporine-like amino acids (MAAs) in the red alga Bostrychia scorpioides. The investigated MAAs, named bostrychines, have only been found in this specific species so far. The developed HPLC-DAD method was successfully applied for the quantification of the major MAAs in Bostrychia scorpioides extracts, collected from four different countries in Europe showing only minor differences between the investigated samples. In the past, several Bostry

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Sıcak, Yusuf, İrfan Öztürk, Bihter Şahin, Dilaycan Çam, Cansel Cakır, and Mehmet Öztürk. "Chemical content profile and antioxidant activity of Rhododendron ponticum extracts." International Journal of Secondary Metabolite 11, no. 3 (2024): 507–13. http://dx.doi.org/10.21448/ijsm.1382522.

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Rhododendron species (Ericaceae) is considered the most diverse group, with more than 1200 species famous for their colorful flowers. Rhododendron, also known as azalea, is a flowering tree in the Ericaceae family. Herein, in vitro antioxidant activities of acetone and methanol extracts of Rhododendron ponticum leaves were studied along with their phenolic contents using High-Performance Liquid Chromatography with a Diode-Array Detector (HPLC-DAD). Antioxidant activity was performed spectrophotometrically using ABTS·+, DPPH·, CUPRAC, and β-carotene/linoleic acid assays. Acetone extract showed

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Szewczyńska, Małgorzata, and Małgorzata Pośniak. "Docetaxel. Determination in workplace air with high performance liquid chromatography." Podstawy i Metody Oceny Środowiska Pracy 34, no. 2(96) (2018): 145–59. http://dx.doi.org/10.5604/01.3001.0012.0763.

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Docetaxel (DCT) is a plant derived cytotoxic from taxane family - mitosis inhibitors. It is used in the treatment of breast, lung and prostate cancer, squamous cell carcinoma of the head and neck, and gastric adenoma. Docetaxel is a highly flammable liquid and health-threatening substance classified as mutagenicity category 2 and reproductive toxicity category 1B. This paper presents a method for measuring docetaxel in the workplace air with HPLC with diode array detector (DAD). The method is based on the adsorption of inhalable fraction of docetaxel aerosol on glass fiber filter, desorption w

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Wang, Yuwen, Shuping Li, Liuhong Zhang, et al. "Chemical Fingerprint Analysis and Simultaneous Determination of Nucleosides and Amino Acids in Kang Fu Xin Liquid by High Performance Liquid Chromatography with Diode Array Detector." Current Pharmaceutical Analysis 16, no. 7 (2020): 831–43. http://dx.doi.org/10.2174/1573412915666190328215231.

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Background and Objective: Kang Fu Xin liquid (KFX) is an official preparation made from the ethanol extract product from P. Americana. The present quality control method cannot control the quality of the preparation well. The aim of the present study is to establish a convenient HPLC method for multicomponents determination combined with fingerprint analysis for quality control of KFX. Methods: An HPLC-DAD method with gradient elution and detective wavelength switching program was developed to establish HPLC fingerprints of KFX, and 38 batches of KFX were compared and evaluated by similarity a

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Reheman, Ayinuer, Haji Akber Aisa, Qing Ling Ma, Dilaram Nijat, and Rahima Abdulla. "Quality Evaluation of the Traditional Medicine Majun Mupakhi ELA via Chromatographic Fingerprinting Coupled with UHPLC-DAD-Quadrupole-Orbitrap-MS and the Antioxidant Activity In Vitro." Evidence-Based Complementary and Alternative Medicine 2018 (2018): 1–13. http://dx.doi.org/10.1155/2018/1035809.

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By merging a high-performance liquid chromatography diode array detector (HPLC-DAD) method with high-performance thin-layer chromatography (HPTLC), an assay was developed for chemical fingerprinting and quantitative analysis of traditional medicine Majun Mupakhi ELA (MME), and constituent compounds were identified using HPLC coupled with UHPLC-DAD-Quadrupole-Orbitrap-MS method. In addition, the antioxidant capacity of MME was assessed based on the ability of components to scavenge radicals using in vitro method. Using a HPLC-DAD method with HPTLC easily validated the chemical fingerprinting re

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Barbosa, Jósquia S., Verônica M. Almeida, Rosilene M. Marçal, and Alexsandro Branco. "RP-HPLC-DAD-MSn Analysis and Butyrylcholinesterase Inhibitory Activity of Barbacenia blanchetii Extracts." Natural Product Communications 10, no. 6 (2015): 1934578X1501000. http://dx.doi.org/10.1177/1934578x1501000646.

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High-performance liquid chromatography (HPLC) coupled to a diode-array detector (DAD) and electrospray ionization mass spectrometry (MS) was applied for the separation and identification of phenolic compounds in the n-hexane, ethyl acetate and methanol crude extract of Barbacenia blanchetii. The MS, MSn and UV data together with chemosystematic evidence allowed the structural characterization of five compounds: tricin, chrysoeriol, epi-gallocatechin, kaempferol 3- O-glucoside and caffeoylquinic acid. At the same time, these extracts were evaluated against butyrylcholinesterase using Ellman's m

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Nuñez-Gómez, Vanesa, Nieves Baenas, Inma Navarro-González, et al. "Seasonal Variation of Health-Promoting Bioactives in Broccoli and Methyl-Jasmonate Pre-Harvest Treatments to Enhance Their Contents." Foods 9, no. 10 (2020): 1371. http://dx.doi.org/10.3390/foods9101371.

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Broccoli is a source of bioactive compounds that provide an important nutritional value. The content of these compounds can vary depending on agronomic and environmental conditions, as well as on elicitation. In this study, three crop trials were carried out to evaluate the effects of the cultivation season, the application of different dosages of methyl-jasmonate (MeJA) on the overall quality and on the total content of bioactive compounds of ‘Parthenon’ broccoli cultivated under the field conditions of southeastern Spain. Color parameters, chlorophyll content, total phenolic compounds, total

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Seo, Chang-Seob, and Mee-Young Lee. "Method Development and Validation for Simultaneous Analysis of Eleven Components for Quality Control of Geumgwesingihwan Using HPLC–DAD and UPLC–MS/MS." Separations 9, no. 8 (2022): 213. http://dx.doi.org/10.3390/separations9080213.

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Geumgwesingihwan (GGSGH) is an oriental herbal formula made by adding Achyranthes bidentate Blume and Plantago asiatica L. to Yukmijiwhanghwan. It has been used for the treatment of edema since ancient times. The purpose of this study is to develop and validate a method for simultaneous quantification of 11 components: gallic acid (1), 5-(hydroxymethyl)furfural (2), geniposidic acid (3), morroniside (4), loganin (5), paeoniflorin (6), acteoside (7), cornuside (8), benzoic acid (9), benzoylpaeoniflorin (10), and paeonol (11), using high-performance liquid chromatography with a diode array detec

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Wang, Ting Xin, Li Yan Zhou, Xiao Yu Zhang, and Hai Xia Jiao. "Analysis of Rhodamine B in Chinese Wolfberry from Market Sale by HPLC." Advanced Materials Research 1010-1012 (August 2014): 826–29. http://dx.doi.org/10.4028/www.scientific.net/amr.1010-1012.826.

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Based on the method of high performance liquid chromatography - diode array detector (HPLC - DAD) ,this experiment rapidly detected rhodamine B of Chinese wolfberry from market sale. Rhodamine B in the samples was dissolved by hexane.And after it was evaporated, it was extracted quantitatively.And then rhodamine B was measured with high performance liquid chromatography (HPLC) detector, using external standard method of peak area for quantitation. Results showed that the test conditions: 80 percent methanol and 20 percent water as mobile phase; sample volume was 20 μL ; column temperature was

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Patyra, Ewelina, and Krzysztof Kwiatek. "In-house validation method for quantification of amoxicillin in medicated feedingstuffs with the use of HPLC-DAD technique." Journal of Veterinary Research 64, no. 3 (2020): 433–38. http://dx.doi.org/10.2478/jvetres-2020-0044.

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AbstractIntroductionA high-performance liquid chromatographic–diode array detector (HPLC-DAD) method for the determination of amoxicillin in medicated feedingstuffs was developed and validated. The method was used to investigate the quality requirements of animal feedingstuffs (declared content of active substance and feed homogeneity).Material and MethodsTwo-gram samples were extracted by potassium phosphate buffer solution. Extracts were filtered and directly analysed by HPLC-DAD without further clean-up. Amoxicillin was separated by acetonitrile and 0.01M phosphate buffer (pH 5.0) on a Phen

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Kaya, Gulen Irem, Derya Cicek, Buket Sarıkaya, Mustafa Ali Onur, and Nehir Unver Somer. "HPLC - DAD Analysis of Lycorine in Amaryllidaceae Species." Natural Product Communications 5, no. 6 (2010): 1934578X1000500. http://dx.doi.org/10.1177/1934578x1000500610.

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Lycorine, the most frequent alkaloid found in Amaryllidaceae plants, has been proven to have various biological activities. Therefore, it is important to quantify this compound in Amaryllidacaeae species. In this study, a reversed-phase high performance liquid chromatographic method has been used and validated for the determination of lycorine in Amaryllidaceae plants. A simple method for the extraction of lycorine in low-mass plant samples was employed utilizing columns pre-packed with diatomaceous earth (Extrelut®). This method was applied to the aerial parts and bulbs of Sternbergia sicula

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Katsa, Maria, Charalampos Proestos, and Efstratios Komaitis. "Determination of Fat Soluble Vitamins A and E in Infant Formulas by HPLC-DAD." Current Research in Nutrition and Food Science Journal 4, Special-Issue-October (2016): 92–96. http://dx.doi.org/10.12944/crnfsj.4.special-issue-october.12.

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A rapid, sensitive and inexpensive method has been developed for the simultaneous determination of fat-soluble vitamins A and E in infant formula (IF) samples. The isolation of fat-soluble vitamins includes a saponification step and an extraction step with petroleum ether. The qualitative and quantitative determination of vitamins was performed by High Performance Liquid Chromatography (HPLC) coupled to Diode Array Detector (DAD). More specifically, method development involved optimization of saponification time, testing of antioxidants, and number of extractions along with the extraction time

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Russo, Venera, Alberto Continella, Carmelo Drago, et al. "Secondary metabolic profiles and anticancer actions from fruit extracts of immature pomegranates." PLOS ONE 16, no. 8 (2021): e0255831. http://dx.doi.org/10.1371/journal.pone.0255831.

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Immature fruits from Punica granatum L. thinning are a neglected side product of pomegranate production with cumbersome disposal costs for farmers. To explore value potential of immature fruits from pomegranate ‘Wonderful’ cultivars, the compositional landscapes and antitumorigenic activities of pomegranate extracts from two different stages of maturation were assessed. Cancer cell proliferation and cytotoxicity was quantified in human lung H1299 and colon HCT116 adenocarcinomas by crystal violet staining, MTS assay and caspase-3 activity. High performance liquid chromatography—diode array det

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Carrillo Heredero, Alicia Maria, Giulia Segato, Simonetta Menotta, et al. "A New Method for Ivermectin Detection and Quantification through HPLC in Organic Matter (Feed, Soil, and Water)." Journal of Analytical Methods in Chemistry 2023 (March 1, 2023): 1–7. http://dx.doi.org/10.1155/2023/6924263.

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Ivermectin is a macrocyclic lactone widely used in veterinary medicine for its broad-spectrum antiparasitic properties. It has been proven to be effective and safe. The purpose of this study was to develop a high-performance liquid chromatography method with a diode array detector for ivermectin screening in feed and water for animal consumption. Furthermore, the objective was to quantify ivermectin levels that were higher than 0.5 mg/kg in solid matrixes and 0.1 mg/kg in water. Doramectin was used as process standard. Samples were extracted using solid phase extraction with silica and C-18 co

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Araya, Michael, Samantha García, and Marcia González-Teuber. "Rapid Identification and Simultaneous Quantification of Aristolochic Acids by HPLC-DAD and Confirmations by MS in Aristolochia chilensis Using a Limited Biomass." Journal of Analytical Methods in Chemistry 2018 (June 6, 2018): 1–8. http://dx.doi.org/10.1155/2018/5036542.

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Six aristolochic acids were identified in the Chilean species Aristolochia chilensis using high-performance liquid chromatography coupled to a diode array detector (HPLC-DAD) and subsequent confirmation with mass spectrometry (MS). The fractions of each signal were collected and injected directly into an Orbitrap mass detector model Q Exactive Focus (Thermo Scientific). The acids extraction was done with 0.10–0.50 g of lyophilized and pulverized sample and concentrated in Soxhlet extraction equipment. The liquid-liquid separations and a subsequent solid phase extraction (SPE) C18 were performe

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Di, Lena Gabriella, del Pulgar Jose Sanchez, Boccia Ginevra Lombardi, Irene Casini, and Nicoli Stefano Ferrari. "Corn Bioethanol Side Streams: A Potential Sustainable Source of Fat-Soluble Bioactive Molecules for High-Value Applications." Foods 2020, no. 9 (2020): 1788. https://doi.org/10.3390/foods9121788.

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This paper reports data from a characterization study conducted on the unsaponifiable lipid fraction of dry-grind corn bioethanol side streams. Phytosterols, squalene, tocopherols, tocotrienols, and carotenoids were quantified by High Performance Liquid Chromatography with Diode-Array Detector (HPLC-DAD) and Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS) in different lots of post-fermentation corn oil and thin stillage collected from a bioethanol plant over a time-span of one year. The concomitant presence of a variegate complex of bioactive molecules with high antioxidant potential

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Acevska, Jelena, Gjoshe Stefkov, Natalija Nakov, Marija Karapandzova, Svetlana Kulevanova, and Aneta Dimitrovska. "Determination of relative response factors of the opium alkaloids with HPLC-DAD." Macedonian Pharmaceutical Bulletin 57 (2011): 37–41. http://dx.doi.org/10.33320/maced.pharm.bull.2011.57.004.

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In this work, a convenient method for determination of relative UV response factors (RRFs) of morphine, codeine, thebaine, oripavine, noscapine and papaverine by high performance liquid chromatography (HPLC) equipped with a diode array detector (DAD) was presented. Pholcodine was selected as the reference compound for calculating the relative response factors of the alkaloids. The separation of all seven compounds was obtained with optimized gradient elution with high pH value of the mobile phase on a reversed phase column with bidentate C18-C18 bonding technology. The RRFs of the alkaloids we

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Hsu, Yi Chyun, Jing Wen Cao, Shao Wei Liao, Chung Yi Chung, Hwa Sheng Gau, and Wen Liang Lai. "Fluorescent Signatures of Organic Matter Attached on Particulate Particle in the Atmosphere Surrounding in Steel Factory." Advanced Materials Research 955-959 (June 2014): 1224–28. http://dx.doi.org/10.4028/www.scientific.net/amr.955-959.1224.

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In this research, the characterization and molecular weight of organic matter, adsorbed on both particle sizes from ambient air in the steel factory, was dissolved into adequate distilled water and analyzed by fluorescent spectrometry and high performance liquid chromatograph (HPLC) equipped with Diode Array Detector (DAD). The components of water-soluble organic compounds (WSOC) attached on both particle sizes, including PM 2.5 μm and PM 2.5-10 μm, were elucidated by the integration of excitation-emission fluorescent matrix (EEFM) with Parallel Factor (PARAFAC) analysis. It reveals that total

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Al-Khadhra, Riyadh Salih. "The determination of common anabolic steroid and stimulants in nutritional supplements by HPLC-DAD and LC-MS." Journal of Chromatographic Science 58, no. 4 (2020): 355–61. http://dx.doi.org/10.1093/chromsci/bmz121.

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Abstract A high-performance liquid chromatography method employing a diode-array detector and mass spectrometry detector was developed, validated and implemented for determining Synephrine, Caffeine, Clenbuterol, Nandrolone, Testosterone and Methylhexaneamine in Nutritional supplements. The use of Nutritional supplements is widespread. Hazards relating to concentration, composition, individual contaminants, supplements interactions as well as positive doping results among athletes present increasing concerns regarding nutritional supplement consuming. The proposed method was validated accordin

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Çankırılıgil, Ekrem Cem, Nermin Berik, and Esen Alp Erbay. "Optimization of hydrolyzation procedure for amino acid analysis in fish meat with HPLC-DAD by Response Surface Methodology (RSM)." Ege Journal of Fisheries and Aquatic Sciences 37, no. 2 (2020): 113–23. http://dx.doi.org/10.12714/egejfas.37.2.01.

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In this research, with the aim of maximizing amino acid content by different hydrolization procedures of fish meat were carried out and analysed with high performance liquid chromatography (HPLC) equipped with diode array detector (DAD). Response surface methodology (RSM) was used to determine factors that used in the experiments. The RSM suggested 16 different hydrolization trials between the ranges of normality as 3 N - 8 N, temperature as 90°C-110°C and duration as 12-24 hours were run. In the following, hydrolysed samples were analysed with HPLC-DAD and obtained data were evaluated with Bo

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Gruznova, Olga Alexandrovna, Dmitry Vyacheslavovich Gruznov, Alexey Borisovich Sokhlikov, et al. "Detection of Fluvalinate Residues in Beeswax and Honey Using High Performance Liquid Chromatography with Diode Array Detector." Current Research in Nutrition and Food Science Journal 13, no. 1 (2025): 440–57. https://doi.org/10.12944/crnfsj.13.1.30.

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Fluvalinate is a synthetic pyrethroid widely used to control honey bee infestations caused by Varroa destructor and Acarapis woodi mites. Contamination of bee products with fluvalinate residues may pose a serious health risk to consumers and is therefore a major concern. HPLC with diode array detector (HPLC-DAD) method was developed for identification and quantification this acaricide in beeswax and honey. The method involves a modified, easy-to-perform liquid-liquid extraction of fluvalinate. As a result of selecting the optimal extracting solvent, it was found that the best extraction of the

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Rosa, Anderson da Silva, Bruna Piaia Ramborger, Maria Luisa Candido Zago, Dandara Fidélis Escoto, Elton Luis Gasparotto Denardin, and Rafael Roehrs. "Determination of pesticides in hydroponic water for environmental phytoremediation." Ciência e Natura 44 (June 13, 2022): e27. http://dx.doi.org/10.5902/2179460x68460.

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This study validated a simple, and fast method by High Performance Liquid Chromatography with Diode Array Detector (HPLC -DAD) for pesticide phytoremediation analysis. The method was developed in water and in a hydroponic medium. Sample extraction and concentration were performed by Solid Phase Extraction (SPE) with Strata C18-E type cartridges. The SPE-HPLC-DAD method was successfully applied in the detection and quantification of quinclorac, 2,4-D, propanil, bentazon, clomazone and tebuconazole in water and hydroponic medium for 14 days. The method presented excellent results with the linear

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Alrhia, Lobaina M., Issam Mohamad, and Sameer Mearouf. "Determination and validation of Tetracycline residues in Poultry by High Performance Liquid Chromatography - Diode Array Detector Technique." Al-Mustansiriyah Journal of Science 30, no. 3 (2019): 38. http://dx.doi.org/10.23851/mjs.v30i3.650.

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The aim of this research is separation and determination of trace Tetracycline residues in Poultry chest, thigh and liver using High Performance Liquid Chromatography - Diode Array Detector (HPLC-DAD), with a mobile phase mixture consisting of acetonitrile: methanol: oxalic acid (0.01M) (25:15:60) and chromatographic column C8. The study was done on 32 live poultry individuals. All individuals were injected in the chest by 1m of Tetracycline standard solutions, then slaughter for analysis throughout four successive days. The injection with 10×103 ppb of Tetracycline showed that the traces of T

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Ali, Hazim M. "Simultaneous Determination of Methyl Nicotinate and Three Salicylic Acid Derivatives in Pain Relief Spray Using HPLC–DAD." Separations 9, no. 4 (2022): 93. http://dx.doi.org/10.3390/separations9040093.

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For the first time, the high-performance liquid chromatography–diode array detector (HPLC–DAD) approach was operated for the simultaneous assessment of methyl nicotinate (MN), methyl salicylate (MS), ethyl salicylate (ES) and 2-hydroxyethyl salicylate (HES) in one pharmaceutical formulation. The limits of detection of MN, HES, MS and ES were found to be 0.0144, 0.0455, 0.0087 and 0.0061 μg/mL. The recovery percentages and relative standard deviations ranged from 93.48 to 102.12% and 0.301 to 6.341% for all active ingredients. Accordingly, the previously described data demonstrate the sensitivi

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Ridhi Joshi, Rishikesh Meena, Preeti Mishra, and Vidya Patni. "RP-HPLC-DAD determination and quantification of Quercetin and Luteolin in plant extracts of Merremia aegyptia and Merremia dissecta." International Journal of Research in Pharmaceutical Sciences 11, no. 3 (2020): 4660–64. http://dx.doi.org/10.26452/ijrps.v11i3.2752.

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Medicinal plants produce various useful metabolites such as alkaloids, flavonoids, tannins etc those are widely used for the preparation of various pharmaceutical products, or as food additives. A simple and precise reverse phase high performance liquid chromatographic (RP-HPLC) seperation method has been developed for determination and quantification of the flavonoids, quercetin and luteolin simultaneously, from methanolic extracts of Merremia aegyptia and Merremia dissecta after optimization of extracting solvent and chromatographic conditions through HPLC coupled to a Diode Array Detector(D

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Jiménez-Nava, Raziel Arturo, Luis Gerardo Zepeda-Vallejo, Fortunata Santoyo-Tepole, Griselda Ma Chávez-Camarillo, and Eliseo Cristiani-Urbina. "RP-HPLC Separation and 1H NMR Identification of a Yellow Fluorescent Compound—Riboflavin (Vitamin B2)—Produced by the Yeast Hyphopichia wangnamkhiaoensis." Biomolecules 13, no. 9 (2023): 1423. http://dx.doi.org/10.3390/biom13091423.

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The yeast Hyphopichia wangnamkhiaoensis excretes a brilliant yellow fluorescent compound into its growth culture. In this study, we isolated and identified this compound using reverse-phase high-performance liquid chromatography-diode array detector (RP-HPLC-DAD) as well as 1H NMR and UV–Vis spectroscopy. Two of the three RP-HPLC-DAD methods used successfully separated the fluorescent compound and involved (1) a double separation step with isocratic flow elution, first on a C18 column and later on a cyano column, and (2) a separation with a linear gradient elution on a phenyl column. The wavel

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Li, Feng, Sen Guo, Shanshan Zhang, et al. "Bioactive Constituents of F. esculentum Bee Pollen and Quantitative Analysis of Samples Collected from Seven Areas by HPLC." Molecules 24, no. 15 (2019): 2705. http://dx.doi.org/10.3390/molecules24152705.

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Bee pollen contains all the essential amino acids needed by humans. China is the largest producer of bee pollen in the world. In the present study, we identified 11 fatty acids in F. esculentum bee pollen oil by GC-MS analysis, and 16 compounds were isolated from F. esculentum bee pollen by column chromatography and identified. A high-performance liquid chromatography-diode array detector (HPLC-DAD) method was established for the quality control of F. esculentum bee pollen. A validated HPLC-DAD method was successfully applied to the simultaneous characterization and quantification of nine main

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Rashid, Azeez* Vinod A. Bairagi Ziyaurrahman Azeez. "A Novel Validated stability indicating QBD based RS method by HPLC for the estimation of impurities of Norethindrone in Norethindrone acetate and their degradation as per ICH Q2 Guideline." International Journal in Pharmaceutical Sciences 2, no. 2 (2024): 297–315. https://doi.org/10.5281/zenodo.10652948.

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A novel and efficient stability indicating reverse phase High performance liquid chromatography with diode array detector (RP-HPLC-DAD) RS analytical method has been developed, optimized and validated for the determination of degradation products and process impurities of Norethindrone in Norethindrone tablets: Nor androstenedione (Imp-A), Norethindrone enolether (Imp-B), Delta-5(6)Norethindrone (Imp-C), Delta-5(10)Norethindrone (Imp-D). The impurities elution and separation were established by optimized gradient chromatographic parameter using Inertial ODS-3V (150 mm x 4.6 mm) 5μ analytica

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Rotondo, Archimede, Giovanna Loredana La Torre, Giovanni Bartolomeo, et al. "Profile of Carotenoids and Tocopherols for the Characterization of Lipophilic Antioxidants in “Ragusano” Cheese." Applied Sciences 11, no. 16 (2021): 7711. http://dx.doi.org/10.3390/app11167711.

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Lipophilic antioxidants such as carotenoids and tocopherols are appreciated components in food because of their potential health benefits. The aim of this study was to describe the composition of these microconstituents in “Ragusano”, a typical Sicilian historical pasta filata cheese, and to compare them during two different production seasons. Specifically, the tocopherols’ composition was evaluated by high-performance liquid chromatography coupled to a fluorescence detector (HPLC-FD); whereas the contents of three main carotenoids were determined by high-performance liquid chromatography cou

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Li, Zhuojun, Cui Wu, Bo Xu, et al. "Gender Discrimination of Flower Buds of Mature Populus tomentosa by HPLC Fingerprint Combined with Chemometrics." International Journal of Analytical Chemistry 2022 (September 29, 2022): 1–10. http://dx.doi.org/10.1155/2022/1281521.

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A high performance liquid chromatography-diode array detector (HPLC-DAD) was used to establish the HPLC fingerprint. Chemometrics methods were used to discriminate against the gender of flower buds of Populus tomentosa based on areas of common peaks calibrated with the HPLC fingerprint. The score plot of principal component analysis (PCA) showed a clear grouping trend (R2X, 0.753; Q2, 0.564) between female and male samples. Two groups were also well discriminated with orthogonal partial least squares-discriminant analysis (OPLS-DA) (R2X, 0.741; R2Y, 0.980; Q2, 0.970). As the hierarchical clust

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