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Journal articles on the topic 'HPLC/F'

Author: Grafiati

Published: 4 June 2021

Last updated: 13 February 2022

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1

Tipton, Peter. "HPLC in Enzymatic Analysis. Edward F. Rossomando , Zdenek Deyl." Quarterly Review of Biology 74, no. 2 (1999): 220. http://dx.doi.org/10.1086/393092.

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2

McFarlane, Andrew G., Linda M. Halchuk, Barry Eng, John Waye, and Mark A. Crowther. "Evaluation of the CapillaryS 2 CE System for Hemoglobinopathy Investigations." Blood 108, no. 11 (2006): 3781. http://dx.doi.org/10.1182/blood.v108.11.3781.3781.

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Abstract Traditional diagnosis of hemoglobinopathies rely on separation and accurate quantification of hemoglobin (Hb) fractions using alkaline and acid electrophoresis, isoelectric focusing (IEF) and/or High Performance Liquid Chromatography (HPLC). Recent reports have suggested capillary electrophoresis (CE) as an alternate method for screening. We evaluated a new CE system the Sebia CapillaryS 2 (Evry Cedex, France) and compared it to IEF and HPLC. For this evaluation samples referred to our laboratory for routine hemoglobinopathy screening or as cord bloods sent for confirmation of a varia

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3

Arpini, Sabrina, Nicola Fuzzati, Andrea Giori, et al. "HPLC-DAD-MS Fingerprint of Andrographis Paniculata (Burn. f.) Nees (Acanthaceae)." Natural Product Communications 3, no. 12 (2008): 1934578X0800301. http://dx.doi.org/10.1177/1934578x0800301208.

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An HPLC-UV fingerprint analysis was developed for the quality evaluation of Andrographis paniculata aerial parts. HPLC-DAD-MS experiments allowed the identification of eleven diterpenes and five flavonoids. Plant material of Indian and Chinese origin was evaluated employing the developed method. The chemical fingerprints of the plant material of different origins do not show significant differences.

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4

Kammann, Ulrike, Clare Askem, Henryka Dabrowska, et al. "Interlaboratory Proficiency Testing for Measurement of the Polycyclic Aromatic Hydrocarbon Metabolite 1-Hydroxypyrene in Fish Bile for Marine Environmental Monitoring." Journal of AOAC INTERNATIONAL 96, no. 3 (2013): 635–41. http://dx.doi.org/10.5740/jaoacint.12-080.

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Abstract The determination of PAH metabolites in fish bile is performed to assess the PAH contamination in fish for environmental monitoring. A growing number of laboratories in Europe use this parameter for national monitoring. However, an international intercomparison between laboratories has not taken place in the last years. Therefore the determination of the PAH metabolite 1-hydroxypyrene was tested in a collaborative trial performed by 10 laboratories from eight countries. Five samples of naturally contaminated fish bile covering different concentration levels were distributed among the

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5

Cotton, Frédéric, Changying Lin, Bernard Fontaine, Béatrice Gulbis, Jacques Janssens, and Françoise Vertongen. "Evaluation of a Capillary Electrophoresis Method for Routine Determination of Hemoglobins A2 and F." Clinical Chemistry 45, no. 2 (1999): 237–43. http://dx.doi.org/10.1093/clinchem/45.2.237.

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Abstract Hemoglobin A2 (Hb A2) and hemoglobin F (Hb F) are important analytes in the diagnosis and follow up of Hb diseases. We evaluated a new capillary zone electrophoresis (CZE) kit for Hb A2 and Hb F measurements. The imprecision ranged from 3% to 6% for Hb A2 and Hb F at physiological and pathological concentrations. The method compared well with cation-exchange HPLC for Hb A2 and Hb F and with anion-exchange chromatography in microcolumns (MAEC), for Hb A2. Nevertheless, higher results were obtained [Hb A2 CZE (%) = 1.233 Hb A2 HPLC − 0.2; Hb A2 CZE (%) = 1.190 Hb A2 MAEC + 0.1; Hb FCZE

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6

Pfeiffer, Christine M., Dan L. Huff, S. Jay Smith, Dayton T. Miller, and Elaine W. Gunter. "Comparison of Plasma Total Homocysteine Measurements in 14 Laboratories: An International Study." Clinical Chemistry 45, no. 8 (1999): 1261–68. http://dx.doi.org/10.1093/clinchem/45.8.1261.

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Abstract Background: Information on interlaboratory variation and especially on methodological differences for plasma total homocysteine is lacking. Methods: We studied 14 laboratories that used eight different method types: HPLC with electrochemical detection (HPLC-ED); HPLC with fluorescence detection (HPLC-FD) further subdivided by type of reducing/derivatizing agent; gas chromatography/mass spectrometry (GC/MS); enzyme immunoassay (EIA); and fluorescence polarization immunoassay (FPIA). Three of these laboratories used two methods. The laboratories participated in a 2-day analysis of 46 pl

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7

Lu, Yingqing, Ji Young Choi, Sang Eun Kim, and Byung Chul Lee. "HPLC-free in situ18F-fluoromethylation of bioactive molecules by azidation and MTBD scavenging." Chemical Communications 55, no. 78 (2019): 11798–801. http://dx.doi.org/10.1039/c9cc04901k.

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Sequential usage of azide and MTBD, which generates pure [<sup>18</sup>F]fluoromethyl tosylate and scavenges unreacted desmethyl precursors, provided an efficient HPLC-free strategy for the radio-synthesis of <sup>18</sup>F-fluoromethylated compounds.

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8

Li, Feng, Sen Guo, Shanshan Zhang, et al. "Bioactive Constituents of F. esculentum Bee Pollen and Quantitative Analysis of Samples Collected from Seven Areas by HPLC." Molecules 24, no. 15 (2019): 2705. http://dx.doi.org/10.3390/molecules24152705.

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Bee pollen contains all the essential amino acids needed by humans. China is the largest producer of bee pollen in the world. In the present study, we identified 11 fatty acids in F. esculentum bee pollen oil by GC-MS analysis, and 16 compounds were isolated from F. esculentum bee pollen by column chromatography and identified. A high-performance liquid chromatography-diode array detector (HPLC-DAD) method was established for the quality control of F. esculentum bee pollen. A validated HPLC-DAD method was successfully applied to the simultaneous characterization and quantification of nine main

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9

Li, Chun Guang, Shu Jun Sheng, Edwin C K. Pang, Brian May, and Charlie Chang Li Xue. "HPLC Profiles and Biomarker Contents of Australian-GrownSalvia miltiorrhiza f. albaRoots." Chemistry & Biodiversity 6, no. 7 (2009): 1077–86. http://dx.doi.org/10.1002/cbdv.200800221.

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10

Mattusch, J., Th Welsch, and G. Werner. "Elektrochemischer Detektor mit einer Kohlefaserb�ndel-Arbeitselektrode f�r die HPLC." Journal f�r Praktische Chemie/Chemiker-Zeitung 334, no. 1 (1992): 49–52. http://dx.doi.org/10.1002/prac.19923340109.

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11

LÓPEZ-EXPÓSITO, IVÁN, FABIO MINERVINI, LOURDES AMIGO та ISIDRA RECIO. "Identification of Antibacterial Peptides from Bovine κ-Casein". Journal of Food Protection 69, № 12 (2006): 2992–97. http://dx.doi.org/10.4315/0362-028x-69.12.2992.

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The objective of the present study was to identify antimicrobial peptides present in several digests of commercial caseins with gastric enzymes. The most active hydrolysate against Escherichia coli ATCC 25922 and Listeria innocua CECT 910T corresponded to a pepsin digest of bovine κ-casein. The protein digest was first separated by semipreparative high-performance liquid chromatography (HPLC), and the most active fractions were again subjected to a second chromatographic step. Finally, identification of the active peptides was carried out by online and offline HPLC–electrospray ionization–tand

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12

Mario, Nathalie, Bruno Baudin, Christian Aussel, and Jacqueline Giboudeau. "Capillary isoelectric focusing and high-performance cation-exchange chromatography compared for qualitative and quantitative analysis of hemoglobin variants." Clinical Chemistry 43, no. 11 (1997): 2137–42. http://dx.doi.org/10.1093/clinchem/43.11.2137.

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Abstract We have developed two assays for complete analysis of hemoglobins (Hbs) in the field of hemoglobinopathies: a high-performance cation-exchange liquid chromatography (HPLC) assay on the weak cation-exchanger Poly Cat A and a two-step capillary isoelectric focusing (CIEF) assay on the neutral-coated capillary from Beckman in a narrow pH gradient. The resolution was satisfactory for both HPLC and CIEF and allowed separation of normal and common abnormal Hbs, i.e., Hb A, Hb F, Hb A2, Hb S, Hb C, and Hb E; slight differences were shown for the resolution of unusual variants such as Hb C-Ha

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13

Li, Yi, Jinhui Zhou, Xiaofeng Xue, et al. "Fluorometric determination of proline in honey by high-performance liquid chromatography after pre-column derivatization with 7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-F)." Analytical Methods 7, no. 18 (2015): 7625–30. http://dx.doi.org/10.1039/c4ay02886d.

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14

Decking, U. K., C. Alves, R. Spahr, and J. Schrader. "2-Fluoroadenosine uptake by erythrocytes and endothelial cells studied by 19F-NMR." American Journal of Physiology-Heart and Circulatory Physiology 266, no. 4 (1994): H1596—H1603. http://dx.doi.org/10.1152/ajpheart.1994.266.4.h1596.

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Transport and phosphorylation of 2-fluoroadenosine (F-AR) were studied in human erythrocytes and porcine aortic endothelial cells by 19F-nuclear magnetic resonance (NMR) spectroscopy. F-AR (590 microM) added to a human erythrocyte suspension (15% hematocrit) was rapidly incorporated into adenine nucleotides at a rate of 38 nmol.min-1.ml red blood cells-1. Intracellular F-AR could be distinguished from extracellular F-AR due to a chemical shift difference of 0.43 +/- 0.03 ppm (n = 5 experiments). Compared with F-AR, fluoro-ATP purified by high-performance liquid chromatography (HPLC) exhibited

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15

Vrancheva, Radka Z., Ivan G. Ivanov, Ina Y. Aneva, Ivayla N. Dincheva, Ilian K. Badjakov, and Atanas I. Pavlov. "Alkaloid profiles and acetylcholinesterase inhibitory activities of Fumaria species from Bulgaria." Zeitschrift für Naturforschung C 71, no. 1-2 (2016): 9–14. http://dx.doi.org/10.1515/znc-2014-4179.

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Abstract GC-MS analysis of alkaloid profiles of five Fumaria species, naturally grown in Bulgaria (F. officinalis, F. thuretii, F. kralikii, F. rostellata and F. schrammii) and analysis of acetylcholinesterase inhibitory activity of alkaloid extracts were performed. Fourteen isoquinoline alkaloids were identified, with the principle ones being protopine, cryptopine, sinactine, parfumine, fumariline, fumarophycine, and fumaritine. Protopine contents, defined by HPLC analysis varied between 210.6 ± 8.8 μg/g DW (F. schrammii) and 334.5 ± 7.1 μg/g DW. (F. rostellata). While all of the investigated

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16

Mercolini, Laura, Roberto Mandrioli, Mario Amore, and Maria Augusta Raggi. "Simultaneous HPLC-F analysis of three recent antiepileptic drugs in human plasma." Journal of Pharmaceutical and Biomedical Analysis 53, no. 1 (2010): 62–67. http://dx.doi.org/10.1016/j.jpba.2010.02.036.

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17

Vortm�ller, Th, and G. W�nsch. "Fluoreszenz-Detektion f�r die Multielement-Bestimmung von Kationen nach HPLC-Trennung." Journal f�r Praktische Chemie/Chemiker-Zeitung 336, no. 1 (1994): 11–15. http://dx.doi.org/10.1002/prac.19943360104.

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18

Schwedt, Georg, and Michael Hauck. "Neues HPLC-Verfahren f�r Cyclamat in Lebensmitteln mit pr�-chromatographischer Derivatisierung." Zeitschrift f�r Lebensmittel-Untersuchung und -Forschung 187, no. 2 (1988): 127–29. http://dx.doi.org/10.1007/bf01042624.

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19

Eltekova, N. A., and Yu A. Eltekov. "High-Performance Liquid Chromatography as Physico-Chemical Method for Study of Adsorption from Solutions." Adsorption Science & Technology 5, no. 1 (1988): 1–12. http://dx.doi.org/10.1177/026361748800500102.

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High-Performance liquid chromatography (HPLC) of anisole and benzaldehyde on macroporous silica (Silochrom C80) with a hydroxylated surface and a surface covered with polyoxyethylene (POE) monolayer have been studied. On the basis of data obtained from liquid adsorption chromatography the adsorption isotherms of aromatic compounds from solutions were calculated. It has been found that adsorption isotherms obtained by HPLC and static adsorption methods coincide for anisole adsorbed either on hydroxylated or modified surfaces. In the case of benzaldehyde adsorption on hydroxylated silica surface

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20

Qazi, Sohail, Domenic Lombardo, and Mamdouh Abou-Zaid. "A Metabolomic and HPLC-MS/MS Analysis of the Foliar Phenolics, Flavonoids and Coumarins of the Fraxinus Species Resistant and Susceptible to Emerald Ash Borer." Molecules 23, no. 11 (2018): 2734. http://dx.doi.org/10.3390/molecules23112734.

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The Emerald Ash Borer (EAB), Agrilus planipennis, Fairmaire, an Asian invasive alien buprestid has devastated tens of millions of ash trees (Fraxinus spp.) in North America. Foliar phytochemicals of the genus Fraxinus (Oleaceae): Fraxinus pennsylvanica (Green ash), F. americana (White ash), F. profunda (Bush) Bush. (Pumpkin ash), F. quadrangulata Michx. (Blue ash), F. nigra Marsh. (Black ash) and F. mandshurica (Manchurian ash) were investigated using HPLC-MS/MS and untargeted metabolomics. HPLC-MS/MS help identified 26 compounds, including phenolics, flavonoids and coumarins in varying amount

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21

Wang, Yanbin, Huan Shen, Li Jiang, Shuo Chen, and Ning Chen. "Comprehensive Analysis of Purity and Glycosylation Status of Chinese Marketed Recombinant and Urinary FSHs." Current Pharmaceutical Analysis 16, no. 6 (2020): 724–29. http://dx.doi.org/10.2174/1573412915666190314123547.

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Background: Urinary or recombinant Follicle-Stimulating Hormones (uFSH and rhFSH) are regularly applied in controlled ovarian stimulation procedure of assisted reproductive technology. Specific activity and purity of these reagents are of great importance since subtle variations in the contents and glycosylation status of FSH may result in differences in clinical efficacy and safety. Objective: The purpose of this study was to comprehensively analyze the FSH contents, glycosylation status and non-specific protein components of the widely used rhFSH Gonal-F and two Chinese marketed FSHs, r-FSH

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22

Harris, Neil S., and David H. K. Chui. "Hemoglobin Variants That Produce Abnormal Elevations In Hb A1c In The Clinical Laboratory: Electrophoretic Patterns, HPLC Analysis and Globin Gene Sequencing." Blood 122, no. 21 (2013): 4695. http://dx.doi.org/10.1182/blood.v122.21.4695.4695.

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Introduction There are two commonly-used methods for measuring Hemoglobin A1c (Hb A1c) – chromatographic and immunochemical. Immunochemical analysis depends on a specific antibody directed towards an epitope that consists of the linked glucose as well as the N-terminal betachain. High-performance liquid chromatography (HPLC)-determined Hb A1c concentrations that are unusually high (above 18%) may be a consequence of a hemoglobinopathy. Standard alkaline and acid gel electrophoresis and even an extended HPLC chromatogram may not fully resolve the abnormal hemoglobin. Globin gene analysis is an

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23

Xiong, Kaihe, Xingling Ma, Na Cao, et al. "Identification, characterization and HPLC quantification of impurities in apremilast." Analytical Methods 8, no. 8 (2016): 1889–97. http://dx.doi.org/10.1039/c5ay01759a.

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A new compound Imp-F was obtained and the structures were elucidated by using spectral data (NMR, MS, and IR); the potential process-related impurities were speculated in the apremilast drug; LC conditions were optimized and an effective HPLC method for the quantitative determination of the potential process-related impurities in apremilast was developed.

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24

Campbell, Andrew, Kori Bradley, Ama Amachong, et al. "Improved Anemia, Organomegaly and Sustained Fetal Hemoglobin Induction within Aging TR2/TR4 Overexpressing UAB Sickle Cell Mice." Blood 120, no. 21 (2012): 3248. http://dx.doi.org/10.1182/blood.v120.21.3248.3248.

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Abstract Abstract 3248 Background: Fetal Hemoglobin (Hgb F)has been shown to improve morbidity and mortality with SCD patients. We recently published that forced expression of TR2/TR4 resulted in 2.5 fold induction of Fetal Hemoglobin and improved phenotypes within adult 2–3 month humanized sickle cell mice (PNAS Campbell and Cui et al 2011). We wanted to determine the if the Hgb F expression remained durable by HPLC within > 6 m/o mice within SCD:TgTR2/TR4 UAB Mice when compared to 2–3 m/o SCD:TgTR2/TR4 UAB Mice. Further, we wanted to determine if the increased Hgb F expression and improve

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25

Fecher, Roger, Jui Choudhuri, Mohammad Barouqa, Seda Tolu, Caterina Minniti, and Morayma Reyes Gil. "Hemoglobin F or: How I Learned to Stop Wondering and Love the Flow Cytometer." American Journal of Clinical Pathology 152, Supplement_1 (2019): S15—S16. http://dx.doi.org/10.1093/ajcp/aqz112.030.

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Abstract Sickle cell disease (SCD) is the most common inherited blood disorder in the United States. It is a hemoglobinopathy that leads to red blood cell (RBC) sickling and a broad range of disease complications including vaso-occlusive crisis, acute chest syndrome, and retinopathy. Hydroxyurea, a drug used to treat SCD, is known to increase expression of hemoglobin F (HbF), a type of hemoglobin normally expressed in infancy; HbF levels between 10% and 20% are associated with decreased vaso-occlusive episodes and improved survival. Hereditary persistent hemoglobin F (HPHF), a typically asympt

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26

Harnor, Suzannah J., Tommy Rennison, Martin Galler, et al. "Synthesis of 3′-deoxy-3′-fluorothymidine (FLT) 5′-O-glucuronide: a reference standard for imaging studies with [18F]FLT." Med. Chem. Commun. 5, no. 7 (2014): 984–88. http://dx.doi.org/10.1039/c3md00381g.

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The de novo synthesis of 3′-deoxy-3′-fluorothymidine-5′-O-glucuronide enabled both the analysis of this reference standard by HPLC and identification of the metabolite in a blood sample from a patient administered [<sup>18</sup>F]FLT.

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27

Dóka, Ottó, Andrea Brunori, Rezső Schmidt, Dane Bicanic, and György Végvári. "Rutin in buckwheat grain meal determined by UV photoacoustic spectroscopy and HPLC." Nova Biotechnologica et Chimica 16, no. 1 (2017): 61–67. http://dx.doi.org/10.1515/nbec-2017-0009.

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AbstractA relatively novel approach for easy and quick determination of rutin in buckwheat grain is suggested. The rutin content of the grain in seven common buckwheat (Fagopyrum esculentum) and six Tartary buckwheat (Fagopyrum tataricum) varieties was investigated by means of UV photoacoustic spectroscopy and HPLC as reference method. The lowest content was found in ‘Botan’ and ‘Bamby’ varieties, while the highest values were obtained in the variety ‘Emka’. Rutin content in grain of all Tartary buckwheat varieties was two orders of magnitude higher than in the other varieties. Rutin content i

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28

Hoornweg, Gerard Ph, Tjipke De Beer, Nel H. Velthorst, and Cees Gooijer. "Forward Degenerate Four-Wave Mixing as a Detection Method in Liquid Separation Systems: Improving Detection Limits by Means of a Fabry—Perot Interferometer." Applied Spectroscopy 51, no. 7 (1997): 1008–11. http://dx.doi.org/10.1366/0003702971941395.

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A simple, small, and low-resolution confocal Fabry–Perot interferometer (CFP) has been developed to improve the signal-to-noise ratio (S/N) in forward degenerate four-wave mixing (F-D4WM) detection, coupled to high-performance liquid chromatography (HPLC). Its appropriateness is based on the difference in coherence between the F-D4WM signal and the background stray light. A detailed description of the specially designed CFP and its performance is presented. The improvement in S/N was calculated from the chromatographic peaks recorded after an HPLC separation of 1- and 2-aminoanthraquinone. The

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29

Kasperkowiak, Małgorzata, Monika Beszterda, Izabela Bańczyk, and Rafał Frański. "Differentiation of bisphenol F diglycidyl ether isomers and their derivatives by HPLC-MS and GC-MS—comment on the published data." Analytical and Bioanalytical Chemistry 413, no. 7 (2021): 1893–903. http://dx.doi.org/10.1007/s00216-021-03157-2.

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AbstractPositional isomers of bisphenol F diglycidyl ether (BFDGE) have been analyzed by high-pressure liquid chromatography-mass spectrometry and by gas chromatography-mass spectrometry (HPLC-MS, GC-MS). Positional isomers of BFDGE derivatives (BFDGEx2H2O, BFDGExH2OxHCl) have been analyzed by HPLC-MS. On the basis of the obtained fragmentation patterns, the elution order of the isomers has been unequivocally determined, in standard solutions and in the sample of liquid obtained after rinsing an empty mackerel fish can with acetonitrile. Under HPLC condition, para,para isomers are eluted first

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30

Yang, Liu, Zhijun Su, Xing Zeng, et al. "Quality Assessment of Fructus xanthii Based on Fingerprinting Using High-Performance Liquid Chromatography." Journal of AOAC INTERNATIONAL 95, no. 4 (2012): 1053–58. http://dx.doi.org/10.5740/jaoacint.11-032.

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Abstract Because almost every traditional Chinese medicine (TCM) is a multicomponent system, QC of TCMs always involves various difficulties. As a current popular quality assessment approach, focusing on qualitative and quantitative analysis of certain compounds contained in herbal medicine has been widely used for the sake of expediency rather than being a practical and realistic way. However, this method does not take the existence of other constituents into account. Comparatively, the chromatographic fingerprint of the components is a more suitable approach to holistically assess the qualit

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31

Sayah, Karima, Nasreddine El Omari, Mourad Kharbach, et al. "Comparative Study of Leaf and Rootstock Aqueous Extracts of Foeniculum vulgare on Chemical Profile and In Vitro Antioxidant and Antihyperglycemic Activities." Advances in Pharmacological and Pharmaceutical Sciences 2020 (September 1, 2020): 1–9. http://dx.doi.org/10.1155/2020/8852570.

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Foeniculum vulgare is a medicinal plant used in Moroccan folk medicine to treat several diseases such as diabetes. The aim of this study was to determine the phenolic bioactive compounds and to evaluate the antioxidant and antihyperglycemic activities of Foeniculum vulgare leaf and rootstock extracts. Phenolic compounds of F. vulgare rootstock and leaf extracts were determined using HPLC-DAD-QTOFMS analysis. The antioxidant activity was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+) radicals. Moreover, the in vitro antih

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32

Nuriddinova, M. R., V. M. Malikov, A. Yu Kushmuradov, and A. I. Saidkhodzhaev. "Investigation of natural compounds by the HPLC method II. HPLC fingerprint method for the epigeal organs of Ferula kuhistanica and F. tenuisecta." Chemistry of Natural Compounds 28, no. 6 (1992): 551–52. http://dx.doi.org/10.1007/bf00630427.

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33

Agzamova, Manzura Adkhamovna, Ravshanjon Muratjanovich Khalilov та Abdulaziz Adilkhanovich Janibekov. "СHROMATOGRAPHIC ANALYSIS OF СYCLOSIVERSIOSIDE F". chemistry of plant raw material, № 2 (10 червня 2021): 267–74. http://dx.doi.org/10.14258/jcprm.2021028314.

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The plants Astragalus pterocephalus growing in Uzbekistan are a source of triterpene glycosides. The main triterpene glycoside, in terms of content, is a cycloartan glycoside – cyclosiversioside F. To obtain an individual biologically active compound cyclosiversioside F with 95% purity, a proposed method involves extraction with methanol, concentration and dilution with an equal volume of water, then followed by a sequential extraction from the aqueous extract with chloroform, ethyl acetate and butanol. Then a chromatographic separation of the purified amount of extractives on a column w

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34

Kim, Hee Young, Meran Keshawa Ediriweera, Kyung-Hwan Boo, Chang Sook Kim, and Somi Kim Cho. "Effects of Cooking and Processing Methods on Phenolic Contents and Antioxidant and Anti-Proliferative Activities of Broccoli Florets." Antioxidants 10, no. 5 (2021): 641. http://dx.doi.org/10.3390/antiox10050641.

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We investigated the effects of cooking (steaming and microwaving) and processing (freeze-drying and hot-air-drying) methods on the antioxidant activity of broccoli florets. 2,2-diphenyl-1-picrylhydrazyl (DPPH•), 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•), and alkyl• free radical scavenging assays were employed to assess anti-oxidant potentials. The cytoprotective effect against oxidative damage induced by H2O2 was studied using hepatocellular carcinoma (HepG2) cells. Anti-proliferative effects were assessed in MCF-7 and MDA-MB-231 breast cancer cells. L-sulforaphane in broc

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35

Meneni, Srinivasarao, and Venkata Sai Prakash Chaturvedula. "Continuation of Reversed-Phase HPLC Analysis Studies of Steviol Glycosides Isolated From Stevia rebaudiana Bertoni." Journal of Food Research 4, no. 2 (2015): 87. http://dx.doi.org/10.5539/jfr.v4n2p87.

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<p>Additional High Performance Liquid Chromatography (HPLC) studies were performed on the nine sweet steviol glycosides reported in Joint Expert Committee on Food Additives (JECFA) namely rebaudioside A, steviolbioside, stevioside, rubusoside, rebaudioside B, rebaudioside C, rebaudioside D, rebaudioside F, and dulcoside A isolated from the leaves of <em>Stevia rebaudiana</em>. Using Reversed-Phase (RP) HPLC method, individual retention times and area for nine naturally occurring <em>ent</em>-kaurane diterpene glycosides of <em>S. rebaudiana</em> have b

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36

Schenk, H. C., M. Höltershinken, A. Tipold, and C. Leithäuser. "Thiaminbestimmung bei der Katze mittels HPLC." Tierärztliche Praxis Ausgabe K: Kleintiere / Heimtiere 39, no. 04 (2011): 243–48. http://dx.doi.org/10.1055/s-0038-1623585.

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Zusammenfassung Gegenstand und Ziel: Thiaminmangel verursacht bei Katzen häufig zentralnervöse Ausfallerscheinungen, ist häufig verbunden mit einer Dysfunktion des Vestibularsystems und kann unbehandelt zum Tod führen. Ziel der Arbeit war, Thiamin direkt mittels High Pressure Liquid Chromatography (HPLC) zu bestimmen und Werte bei gesunden und kranken Katzen zu evaluieren. Material und Methoden: In Blutproben von 193 Katzen wurden mittels HPLC die Konzentrationen an Thiamindiphosphat und Gesamtthiamin bestimmt. Zur Auswertung der Daten erfolgte eine retrospektive Einteilung der Katzen in folge

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Ou, C. N., and C. L. Rognerud. "Rapid analysis of hemoglobin variants by cation-exchange HPLC." Clinical Chemistry 39, no. 5 (1993): 820–24. http://dx.doi.org/10.1093/clinchem/39.5.820.

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Abstract We investigated the use of a 3.5 x 0.46 cm HPLC column packed with 5-microns particles of porous (100 nm) silica coated with polyaspartic acid for hemoglobin analysis. A 13-min gradient was produced between two mobile phases. The method is capable of separating more than 35 commonly encountered hemoglobin variants within 12 min. Hemoglobin variants identified include Bart's, acetyl F, H, A1c, F, Camden, N-Baltimore, J-Baltimore, N-Seattle, Grady, Fannin-Lubbock, A G-Georgia, Lepore-Baltimore, P-Galveston, G-Coushatta, Lepore-Boston, E, Osu Christiansborg, A2, G-Philadelphia, Korle Bu,

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Dong, H. J., Z. J. Zhang, J. Yu, Y. Liu, and F. G. Xu. "Chemical Fingerprinting of Andrographis paniculata (Burm. f.) Nees by HPLC and Hierarchical Clustering Analysis." Journal of Chromatographic Science 47, no. 10 (2009): 931–35. http://dx.doi.org/10.1093/chromsci/47.10.931.

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Mosca, Andrea, Assunta Carpinelli, Rita Majavacca, et al. "An evaluation of the Diamat HPLC analyser for simultaneous determination of haemoglobins A2and F." Journal of Automatic Chemistry 11, no. 6 (1989): 273–79. http://dx.doi.org/10.1155/s1463924689000520.

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Some minor limitations emerged: the most relevant concerns Hb A1c, which is overestimated with respect to a reference method (y = 1.217x + 0.16; N = 79; r = 0.9235). A probable interference from labile fractions is responsible for this Hb A1cinaccuracy.

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Mercolini, Laura, Maria Addolorata Saracino, Francesca Bugamelli, et al. "HPLC-F analysis of melatonin and resveratrol isomers in wine using an SPE procedure." Journal of Separation Science 31, no. 6-7 (2008): 1007–14. http://dx.doi.org/10.1002/jssc.200700458.

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PRINZENBERG, EVA-MARIA, PATRICIA ANGLADE, BRUNO RIBADEAU-DUMAS та GEORG ERHARDT. "Biochemical characterization of bovine αs1-casein F and genotyping with sequence-specific primers". Journal of Dairy Research 65, № 2 (1998): 223–31. http://dx.doi.org/10.1017/s0022029997002690.

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Bovine αs1-casein F (αs1-CN F) was found in a genetic resource of Deutsches Schwarzbuntes Niederungsrind cows at a frequency of 0·009. Biochemical characterization of this new variant was obtained by automated sequencing of reversed-phase HPLC-separated tryptic peptides of αs1-CN F and αs1-CN B. αs1-CN F was found to be a subtype of αs1-CN B with a single amino acid substitution (SerP/Leu) in position 66. DNA sequencing revealed a C/T transition in position 8418 of the gene. Sequence-specific primers were designed to perform an allele-specific polymerase chain reaction for detection of αs1CnF.

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Journal, Baghdad Science. "Extraction and Purification of Indole aectic acid from locale isolate Fusarium oxysporum(F2)." Baghdad Science Journal 5, no. 1 (2008): 27–37. http://dx.doi.org/10.21123/bsj.5.1.27-37.

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Indole acetic acid (IAA) produced from F. oxysporum (F2) was purified by several steps included extraction by cold ethyl acetate ; Column chromatography using silica gel and TLC chromatography . The pure indole acetic acid (IAA) which produce by F. oxysporum (IAA) was tested by ultraviolet spectra at (200-300)nm ; and appear that the maximum absorbance at 229nm , the high performance liquid chromatography (HPLC) used to test the purity of the indole acetic acid and the results showed one peak at appearance time 3.822 min

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Lambert, Christopher, Mohammad Javad Pourmoghaddam, Marjorie Cedeño-Sanchez, et al. "Resolution of the Hypoxylon fuscum Complex (Hypoxylaceae, Xylariales) and Discovery and Biological Characterization of Two of Its Prominent Secondary Metabolites." Journal of Fungi 7, no. 2 (2021): 131. http://dx.doi.org/10.3390/jof7020131.

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Hypoxylon, a large, cosmopolitan genus of Ascomycota is in the focus of our current poly-thetic taxonomic studies, and served as an excellent source for bioactive secondary metabolites at the same time. The present work concerns a survey of the Hypoxylon fuscum species complex based on specimens from Iran and Europe by morphological studies and high performance liquid chromatography coupled to mass spectrometry and diode array detection (HPLC-MS-DAD). Apart from known chemotaxonomic markers like binaphthalene tetrol (BNT) and daldinin F, two unprece-dented molecules were detected and subsequen

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Aryani, Toetik. "PENGUJIAN VALIDASI ANALISIS KADAR ANDROGRAFOLID SECARA KROMATOGRAFI CAIR KINERJA TINGGI (KCKT) DENGAN ELUASI GRADIEN TERHADAP EKSTRAK HERBA SAMBILOTO (ANDROGRAPHIS PANICULATA NESS)." Berkala Penelitian Hayati 11, no. 1 (2005): 73–76. http://dx.doi.org/10.23869/bphjbr.11.1.200512.

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Andrographolide of Andrographis paniculata Nees have been isolated and determined Extraction was carried out by maceration with ethanol as solvent. The concentration of Andrographolide was determined by HPLC method. The eluation was carried by out gradiently using methanol: water as an eluent and UV spectrophotometer at maks 228 as detector. The result of HPLC analysis are selectivity more than 1.2–1.5, r was 0.9937, precision was KV less than 10 percent; accuracy more than 90 percent, DL was 0.075, QL was 0.50 and PW less than F table. Andrographolide content of Andrographis paniculata Nees f

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Shet, L. S., A. R. Shelar, and F. V. Manvi. "Synthesis of New Chiral and Nonchiral Pyrido [3,2-e],[1,3,4] oxadiazine Derivatives." E-Journal of Chemistry 7, no. 1 (2010): 149–56. http://dx.doi.org/10.1155/2010/791796.

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A chiral series of 6,7-dichloro-3-[3-(2,2-dihalovinyl)-2,2-dimethylcyclopropyl] -1H-pyrido [3,2-e],[1,3,4] oxadiazines(a-f)and a nonchiral series of 3-substituted, 6,7-dichloro-1H-pyrido [3,2-e][1,3,4] oxadiazines(a-g)have been synthesized. The synthesized compounds were characterized by IR,1H NR, HPLC and mass spectral data.

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Hayes, M., C. Stanton, H. Slattery, et al. "Casein Fermentate of Lactobacillus animalis DPC6134 Contains a Range of Novel Propeptide Angiotensin-Converting Enzyme Inhibitors." Applied and Environmental Microbiology 73, no. 14 (2007): 4658–67. http://dx.doi.org/10.1128/aem.00096-07.

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ABSTRACT This work evaluated the angiotensin-converting-enzyme (ACE)-inhibitory activities of a bovine sodium caseinate fermentate generated using the proteolytic capabilities of the porcine small intestinal isolate Lactobacillus animalis DPC6134 (NCIMB deposit 41355). The crude 10-kDa L. animalis DPC6134 fermentate exhibited ACE-inhibitory activity of 85.51% (±15%) and had a 50% inhibitory concentration (IC50) of 0.8 mg protein/ml compared to captopril, which had an IC50 value of 0.005 mg/ml. Fractionation of the crude L. animalis DPC6134 fermentate by membrane filtration and reversed-phase h

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Azis Ambar, Abdul, Henny Setyawati, and Nur Ilmi. "Interaction of Physiology Character - Secondary Metabolic of Fusarium oxysporum on Tomatoes of Fusarium Wilt Symptom." Materials Science Forum 967 (August 2019): 95–100. http://dx.doi.org/10.4028/www.scientific.net/msf.967.95.

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Fusarium oxysporum (F. oxysporum) is the pathogen that caused fusarium wilt diseases on the tomatoes. The rise of the symptom was caused by secondary metabolic produced by F. oxysporum. The associated with the level of secondary metabolic pathogenesis that cause symptoms of wilt on tomatoes, but secondary metabolic excreted by F. oxysporum was not necessarily wilt in a plant. The phenomenon caused F. oxysporum producing secondary metabolic in the different concentration, either its quantity or quality. The nature of physiology being tested, observed by growing 4 isolates using a medium PDA on

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Patel, Niren, Hanfang Zhang, Lina Zhuang, et al. "Diagnostic Challenge Presented by “Hb F only” Pattern In Newborn Screening for Hemoglobinopathies: Approach to Accurate Diagnosis." Blood 116, no. 21 (2010): 5173. http://dx.doi.org/10.1182/blood.v116.21.5173.5173.

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Abstract Abstract 5173 Newborn screening for hemoglobinopathies has been proven to be an effective means of early diagnosis of clinically significant hemoglobinopathies such as sickle cell syndromes with early recruitment to clinical care and resultant reduction in morbidity and mortality. Neonatal screening for hemoglobinopathies is currently performed in 50 states utilizing screening methods such as hemoglobin electrophoresis or isoelectric focusing (IEF) complemented by High Performance Liquid Chromatography (HPLC) in a large number of states. These two methods yield accurate diagnosis of h

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Lu, Dan, Hua He, Bin Wu, and Shanjing Yao. "Cytotoxic Effect on Cancer Cells and Structural Identification of Phenols from Spatholobi Caulis by HPLC-ESI-MSn." Natural Product Communications 4, no. 6 (2009): 1934578X0900400. http://dx.doi.org/10.1177/1934578x0900400614.

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To find fractions from Spatholobi caulis with cytotoxic effects on cancer cell lines, screening tests were carried out using the SRB assay on the HL60 cell line. Further investigation with HL60 and another four human cancer cell lines (KB, K562, MCF-7 and Hep G2), revealed a dose-dependent response. High-performance liquid chromatography coupled with electrospray ionization-tandem mass spectrometry (HPLC- ESI-MSn) was used to isolate and identify the active constituents from the active fraction. Three fractions (F-IV, F-V and F-VII) showed in vitro cytotoxicity. F-V inhibited the growth of can

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Dobrila, Ludy, Tracy Zhu, Dan Zamfir, et al. "Detection of Hemoglobin (Hb) Variants By HPLC Screening in Cord Blood Units (CBU) Donated to the National Cord Blood Program (NCBP)." Blood 128, no. 22 (2016): 2182. http://dx.doi.org/10.1182/blood.v128.22.2182.2182.

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Abstract Purpose. To evaluate the frequency and types of Hb abnormalities in CBU collected by the NCBP at the New York Blood Center. Background and Methods. Established in 1992, NCBP has collected and tested over 76,000 ethnically diverse CBU and manages an inventory of over 60,000 clinical grade CB products, including the first FDA-licensed (HEMACORD®). NCBP has released over 5,600 CBU for transplantation worldwide. To prevent transplants from donors affected with clinically significant hemoglobinopathy (HP), current Good Tissue Practices (cGTP) require Hb screening of all CBU before they are

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