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1

Albishri, Hassan Mabrook. "High-temperature HPLC of pharmaceutical compounds." Thesis, Loughborough University, 2007. https://dspace.lboro.ac.uk/2134/36130.

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In this thesis, we established methods that use high-temperature HPLC for the efficient separation of different groups of pharmaceutical compounds. These groups are cephalosporins, non-steroidal anti-inflammatory (NSAID), and diuretic drugs. The effect of changes of the temperature of the column was studied with respect to various chromatographic parameters: retention factors, thermodynamic properties, selectivity, efficiency and peak shape. Three columns (Pathfinder, Xterra RP18, and Selerity's Blaze 200) were examined for the analysis of cephalosporin drugs under high temperature reversed ph
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2

Scholtzova, Angela. "Scale up and modelling of HPLC." Thesis, University College London (University of London), 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.368109.

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3

Walker, Roderick Bryan. "HPLC analysis and pharmacokinetics of cyclizine." Thesis, Rhodes University, 1995. http://hdl.handle.net/10962/d1003279.

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The investigations detailed in this dissertation have been conducted to address the paucity of pharmacokinetic information, in published literature, pertaining to cyclizine. The areas of investigation have included the selective quantitation of both cyclizine and its demethylated metabolite, norcyclizine in serum and urine, assessment of stability of both compounds in stored biological samples, dosage form analysis, dissolution rate testing of tablets, and bioavailability and pharmacokinetics following administration of an intravenous solution, and tablets to humans. High-performance liquid ch
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4

Benson, Andrew James. "High-performance liquid chromatography (HPLC) and high-performance liquid chromatography mass spectrometry (HPLC/MS) for the analysis of date rape drugs." FIU Digital Commons, 2002. http://digitalcommons.fiu.edu/etd/1602.

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The drugs studied in this work have been reportedly used to commit drug-facilitated sexual assault (DFSA), commonly known as "date rape". Detection of the drugs was performed using high-performance liquid chromatography with ultraviolet detection (HPLC/UV) and identified with high performance-liquid chromatography mass spectrometry (HPLC/MS) using selected ion monitoring (SIM). The objective of this study was to develop a single HPLC method for the simultaneous detection, identification and quantitation of these drugs. The following drugs were simultaneously analyzed: Gamma-hydroxybutyrate (GH
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5

Weddle, Carolyn A. "Optimization of HPLC techniques for separation of oxidized phosphatidylcholines." Virtual Press, 2005. http://liblink.bsu.edu/uhtbin/catkey/1319835.

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In cellular studies of patients with lipid related disorders such as mammary cancers, leukemia, and artheroscierosis, separation of molecular species of oxidized phosphatidylcholine (PC) can be an important assistance in research or diagnosis. Goals of this project were to optimize separation of oxidized and unoxidized PC molecular species in a single HPLC chromatogram followed by in line identification of hydroperoxides. Oxidized egg PC's were produced using UV light exposure in air. Separations were performed on an Ultrasphere ODS column and an Asahipak ODPVA column using a Waters 2695 syste
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6

Edwardson, P. A. D. "High Performance Liquid Chromatography of polynucleotides and proteins." Thesis, London School of Hygiene and Tropical Medicine (University of London), 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.376534.

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7

Lowe, Christian T. "Retention characteristics of water-soluable calixarene modified mobile phases in HPLC /." Connect to the full text of the document, 1998. http://www.ohiolink.edu/etd/view.cgi?ysu997550028.

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8

Trusova, Tatyana. "Quantitative estimation of bile acid conjugates in human bile using HPLC /." Connect to online version, 1995. http://hdl.handle.net/1989/3555.

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9

Meiby, Elinor, Zetterberg Malin Morin, Sten Ohlson, Hernández Víctor Agmo, and Katarina Edwards. "Immobilized lipodisks as model membranes in high-throughput HPLC-MS analysis." Uppsala universitet, Analytisk kemi, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-197337.

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Lipodisks, also referred to as polyethylene glycol (PEG)-stabilized bilayer disks, have previously been demonstrated to hold great potential as model membranes in drug partition studies. In this study, an HPLC-MS system with stably immobilized lipodisks is presented. Functionalized lipodisks were immobilized on two different HPLC support materials either covalently by reductive amination or by streptavidin-biotin binding. An analytical HPLC column with immobilized lipodisks was evaluated by analysis of mixtures containing 15 different drug compounds. The efficiency, reproducibility, and stabil
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10

Ritchie, Harald John. "Development of new packing materials for high performance liquid chromatography." Thesis, University of Edinburgh, 1987. http://hdl.handle.net/1842/14283.

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11

Narine, Raymond. "A novel microcomputer-controlled transport detector for high-performance liquid chromatography." Thesis, King's College London (University of London), 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.281718.

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12

張雅茗. "營實HPLC指紋圖譜研究初探". HKBU Institutional Repository, 2013. http://repository.hkbu.edu.hk/etd_ra/1364.

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13

Smith, Anita Mohler. "Objective judgement of cheese varieties by multivariate analysis of HPLC profiles." Thesis, University of British Columbia, 1987. http://hdl.handle.net/2429/26535.

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An objective analytical method was developed to characterize the taste profiles of five cheese varieties. Nonvolatile water extracts of Cheddar, Edam, Gouda, Swiss, and Parmesan cheeses were analyzed by high performance liquid chromatography (HPLC) with a reversed phase column. The HPLC operating conditions were determined with Mapping Super-Simplex followed by Centroid Mapping Optimization. A ternary gradient elution system was used with an Adsorbosphere C8 column to resolve a maximum number of components. The optimum solvent volume ratio was 96.8 : 1.2 : 2.0 for trifluoroacetic acid (0.1%),
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14

Shi, Yunqing Nancy. "¹H NMR and HPLC studies of tetraarylporphyrin atropisomers." Scholarly Commons, 1993. https://scholarlycommons.pacific.edu/uop_etds/2250.

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This thesis includes NMR studies of free base meso-tetra(otolyl) porphyrin (TTP) and meso -tetraphenylporphyrin (TPP) and their dications protonated by trifluoroacetic acid (TFA). The chemical shift changes of the -NH resonance are very unusual and have never been reported. At the beginning of the titration, the N-H resonances broaden considerably but do not shift; when the ratio of [TFA]/[TTP] or [TFA]/[TPPf are over 2, the N-H resonances shift markedly to lower field by as much as 1.6 ppm. At acid levels well above the equivalence point, the NH resonances moves back to higher field. Moreover
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15

Hirschl, Rhonda Sue. "Synthesis and characterization of HPLC stationary phases using 4-tert-butylcalix[n]arenes /." Connect to online version at Digital.Maag, 1998. http://hdl.handle.net/1989/4787.

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16

Forssén, Patrik. "Adsorption isotherm parameter estimation in nonlinear liquid chromatography /." Uppsala : Acta Universitatis Upsaliensis : Univ.-bibl. [distributör], 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-5971.

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17

Watson, Richard Charles. "Studies of reversed phase high performance liquid chromatography (RP-HPLC) stationary phases." Thesis, University of Nottingham, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.338492.

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18

Witte, Dirk Theodoor. "High performance liquid chromatography for direct and indirect enantiomeric separations of chiral drugs." [S.l. : [Groningen : s.n.] ; University Library Groningen] [Host], 1992. http://irs.ub.rug.nl/ppn/297969609.

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19

Li, F. "Studies of haem biosynthesis and metabolism by high-performance liquid chromatography." Thesis, University of Cambridge, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.378943.

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20

Lien, Wan-Fu. "Separation of Transition Metal Ions by HPLC, Using UV-VIS Detection." Thesis, North Texas State University, 1987. https://digital.library.unt.edu/ark:/67531/metadc501145/.

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HPLC has been used and can quickly determine several ions simultaneously. The method of determination described for transition metals [Cr(III), Fe(III), Ni(II), Co(II), Cu(II), Zn(II), Cd(II), Mn(II)] and [Ca(II), Pb(II)] using HPLC with UV-VIS detection is better than the PAR complexation method commonly used. The effects of both eluent pH and detector wavelength were investigated. Results from using different pHs and wavelengths, optional analytical conditions for the separation of [Ni(II), Co(II), Cu(II)], [Cr(III), Fe(III), Ca(II), Ni(II), Cu(II)], and [Ca(II), Zn(II), Pb(II)] in one injec
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21

Juaristi, Ane Iruretagoiena. "HPLC-FL for determination of hormones in bivalves." Master's thesis, Universidade de Aveiro, 2014. http://hdl.handle.net/10773/14301.

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Mestrado em Química - Química Analítica e Qualidade<br>The main objective of this work was the application of an isocratic high performance liquid chromatography (HPLC) method coupled with a fluorescence detector to find the best conditions for the separation and quantification of 17β-estradiol (E2) and 17α-ethinyl estradiol (EE2) hormones in bivalve samples. A reversed phase column (Kromasil 100 C18, 5μm, 15 x 0.21cm), was employed with a fluorescence detector operating at excitation and emission wavelengths of 280nm and 305nm, respectively. A calibration curve was obtained with a good correl
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22

Forage, Nazhat George. "RP-HPLC separation and kinetics of the decomposition products of tryptophan amadori compound." Thesis, McGill University, 1990. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=59880.

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Amadori rearrangement product (ARP) of tryptophan with glucose was synthesized according to a published procedure, and its decomposition was studied at two different concentrations and at two temperatures, 110$ sp circ$C and 140$ sp circ$C over a period of 6 hrs. A RP-HPLC system was developed to separate and quantitate the decomposition products of the ARP. The results indicated that, the ARP can decompose to form the following products, hydroxymethylfurfural (HMF), maltol, indole, $ beta$-carbolines (norharman and harman) and tryptophan. Further, using the same analytical method, the followi
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23

Klute, Robert Cragg. "Enantiomeric separations by HPLC : temperature, mobile phase, flow rate and retention mechanism studies /." This resource online, 1993. http://scholar.lib.vt.edu/theses/available/etd-06062008-171241/.

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24

Králová, Radka. "Stanovení léčiv v pitných vodách metodou HPLC." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2015. http://www.nusl.cz/ntk/nusl-217167.

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The aim of the diploma thesis is the determination of macrolide antibiotics in drinking water by using of high performance liquid chromatography. Erythromycin and clarithromycin were selected such as representative macrolides due to frequently prescribed pharmaceuticals in this time. Solid phase extraction (SPE) by using of Oasis HLB cartridges was applied for pre concentration and purification of chosen analytes in real samples of drinking water. Optimalization of method and analysis were performed by using of high performance liquid chromatography with mass spectrometry detection (HPLC-MS).
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25

Hayteas, David Lawrence. "HPLC analysis of myoglobin tryptic peptides from selected species of cetaceans." PDXScholar, 1990. https://pdxscholar.library.pdx.edu/open_access_etds/4086.

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Due to the large gaps in the fossil record, the evolutionary history of the mammalian order Cetacea is incomplete and controversial. Increasingly researchers are utilizing molecular and biochemical procedures to supplement cetacean paleontology. One of these methods is the comparison of amino acid sequences of myoglobin among species of this order. since this method is time-consuming and expensive, an alternative procedure is desirable.
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26

Shao, Peimin. "SFE Fractionation and RP-HPLC Characterization of Aquatic Fulvic Acid." Thesis, University of North Texas, 1994. https://digital.library.unt.edu/ark:/67531/metadc500352/.

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The Supercritical Fluid Extraction (SFE) technique was used to fractionate Suwannee River reference fulvic acid (FA). The fractions were characterized by gas chromatography (GC) and reversed-phase high performance liquid chromatography (RP-HPLC). A SFE fractionation method was developed using stepwise gradient of supercritical CO₂ and methanol. Three FA fractions were separated. The average mass recovery was 102% with the coefficient of variation of 2.8%. The fractionation dynamics and the difference in the ratios of UV absorption to fluorescence emission indicate the real fractionation of the
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27

Srisopa, Anong. "Development of porous polymeric stationary phase materials for application in high temperature HPLC." Thesis, University of East Anglia, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.539369.

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28

Ansong, Godfred. "Analysis of plant polyphenols by high performance liquid chromatography/mass spectrometry and protein binding." Oxford, Ohio : Miami University, 2004. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=miami1083081905.

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29

Patel, Hasmukh B. "Reversed-phase high-performance liquid chromatography : some basic studies and application in microbial transformation." Thesis, University of Bath, 1987. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.379158.

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30

Eliser, Erica E. "Synthesis and Characterization of Amino-derived t-butyl-calix[4]arene Bonded Phases for HPLC." Youngstown State University / OhioLINK, 2001. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1008341180.

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31

Li, Cheuk Fai. "Studies of flow injection system for micelle-assisted preconcentration of PAHs coupled with HPLC." HKBU Institutional Repository, 2009. http://repository.hkbu.edu.hk/etd_ra/1059.

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32

Soor, Amritpal. "Group separation of complex mixtures by normal-phase high performance liquid chromatography and analysis by gas chromatography." [S.l. : s.n.], 2008. http://nbn-resolving.de/urn:nbn:de:bsz:289-vts-64791.

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33

Benešová, Markéta. "Stanovení léčiv pomocí HPLC s různými typy detektorů." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2011. http://www.nusl.cz/ntk/nusl-216704.

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This diploma thesis deals with the determination of macrolide antibiotics in wastewater, especially with erythromycin, clarithromycin and roxithromycin. In this time are these pharmaceuticals prescribed quite frequently. Solid phase extraction (SPE) was used for the isolation and the purification of selected analytes from an aqueous matrix; as the suitable procedure was found the using Oasis HLB cartridges. High performance liquid chromatography with mass spectrometry detection (HPLC-MS) was optimized for its analysis of selected pharmaceuticals. The optimized method was used for the determina
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34

Wang, Yu. "HPLC method development for the evaluation of pheromones from the dwarf African clawed frog Hymenochirus." Scholarly Commons, 2003. https://scholarlycommons.pacific.edu/uop_etds/587.

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35

DONALD, GREGORY THOMAS. "Model Chiral Ionic Liquids for High Performance Liquid Chromatography Stationary Phases." University of Cincinnati / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1214325450.

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36

Blais, Jean-Simon. "HPLC-AAS interfaces for the determination of ionic alkyllead, arsonium and selenonium compounds." Thesis, McGill University, 1990. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=74637.

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Three direct interfaces for coupling high performance liquid chromatography (HPLC) with atomic absorption spectrometry (AAS) were developed and optimized for the determination of ionic organolead, organoselenium and organoarsenic compounds. The first all-quartz interface consisted of a thermospray nebulizer and a flame microatomizer in which ionic alkyllead analytes (R$ sb{ rm n}$Pb$ sp{ rm (4-n)+};$ R = CH$ sb3,$ C$ sb2$H$ sb5)$ were atomized by a methanol (from HPLC eluent)-oxygen kinetic flame, and channeled in a quartz tube (atom keeper) mounted into the AAS optical beam. Alternately, the
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37

Brannegan, Daniel Robert. "Analysis of Ethoxyquin and its Oxidation Products using Supercritical Fluid Extraction and High Performance Liquid Chromatography with Chemiluminescent Nitrogen Detection." Thesis, Virginia Tech, 2000. http://hdl.handle.net/10919/31575.

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Ethoxyquin is an antioxidant commonly used to preserve vitamins and lipids in various food products and animal feeds. The extraction and determination of ethoxyquin is becoming increasingly important as products, which are labeled as "natural" are becoming more common. The present method of determination only ensures that ethoxyquin values are below 10-20 parts per million. Therefore, advances are needed in methods of extraction and analysis in order to lower the detection limits in various products. The first part of this research investigates the use of supercritical fluids in the extrac
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38

Ganti, Satyakala. "DEVELOPMENT OF HPLC METHODS FOR PHARMACEUTICALLY RELEVANT MOLECULES; METHOD TRANSFER TO UPLC: COMPARING METHODS STATISTICALLY FOR EQUIVALENCE." Diss., Temple University Libraries, 2011. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/118587.

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Chemistry<br>Ph.D.<br>High Pressure Liquid Chromatography (HPLC) is a well-known and widely used analytical technique which is prevalent throughout the pharmaceutical industry as a research tool. Despite its prominence HPLC possesses some disadvantages, most notably slow analysis time and large consumption of organic solvents. Ultra Pressure Liquid Chromatography (UPLC) is a relatively new technique which offers the same separation capabilities of HPLC with the added benefits of reduced run time and lower solvent consumption. One of the key developments which facilitate the new UPLC technology
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39

Huyghues-Despointes, Alexis. "Synthesis, characterization, and approaches to the analysis by HPLC-THG-AAS of trimethylselenonium, selenoniumcholine and selenoniumacetylcholine cations." Thesis, McGill University, 1991. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=59977.

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Selenonium cations are electron deficient species in which the central selenium atom is bonded to three carbon chains (aryl or alkyl). Trimethylselenonium iodide was synthesized by reaction of methyllithium with metallic selenium to produce methylselenolithium which was, in turn, reacted with the appropriate alkylbromide. The selenide thus formed was further methylated at the selenium atom with methyl iodide in methanol in the presence of sodium tetraphenylborate. After several recrystallizations the selenonium analytes were characterized by AAS, FT-IR, $ sp1$H-NMR, $ sp{13}$C-NMR, FAB-MS and
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40

Suen, Kin-wah, and 孫建華. "Validation of a HPLC assay for porphobilinogen synthase in human erythrocytes for use in the clinical laboratory." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2004. http://hub.hku.hk/bib/B29624897.

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(Uncorrected OCR) Abstract Porphobilinogen (PBG) synthase condenses two molecules of aminolaevulinic acid (ALA) to form PBG in heme biosynthesis. The enzyme activity is sensitive to inhibition by heavy metals such as lead. It can act as a biological indicator of chronic lead POis~r\g to identify the risk group, especially in children, so that early treatment can be given to prevent possible permanent damages. A reversed-phase ion-pair HPLC analytical method for the assay of the PBG synthase activity based on detection of PBG production has been validated. A Hypersil CN column (1
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41

Palmero, David A. "Comparison of cyclic voltammetry and HPLC for the determination of phenol in over-the counter sore throat sprays /." View abstract, 1999. http://library.ctstateu.edu/ccsu%5Ftheses/1540.html.

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Thesis (M.S.)--Central Connecticut State University, 1999.<br>Thesis advisor: James V. Arena, Ph. D. " ... in partial fulfillment of the requirements for the degree of Master of Science in Chemistry." Includes bibliographical references (leaf 48).
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42

Almassalkhi, Brittany A. "Arsenic Speciation, Detection, and Quantification in Drinking Water using High Performance Liquid Chromatography and Inductively Coupled Plasma Mass Spectrometry." University of Cincinnati / OhioLINK, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1258580087.

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43

Hanan, Nathan, and David Nix. "Extending the Stability of Intravenous Ampicillin." The University of Arizona, 2012. http://hdl.handle.net/10150/614496.

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Class of 2012 Abstract<br>Specific Aims: To assess the chemical stability of ampicillin for injection in normal saline at pH values ranging from 5 to 6. Methods: A stability-indicating high performance liquid chromatography (HPLC) method was developed and used to determine the stability of ampicillin for injection in normal saline following buffering with sodium acetate and acid adjustment with HCl at pH values of 5, 5.5, and 6. To confirm that the assay was stability-indicating, ampicillin trihydrate reference standard (1 mg/mL) was exposed to alkali, acid, and oxidative stress condition
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44

Dhoot, Gaurav. "Estimation of eugenol diffusion coefficient in LLDPE using FTIR-ATR flow cell and HPLC techniques." Diss., Connect to online resource - MSU authorized users, 2008.

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45

Anaia, Grazielle de Campos. "Determinação multirresidual de pesticidas por HPLC no contexto de exposição ocupacional." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/46/46136/tde-23012015-101815/.

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Um método para determinação de praguicidas das classes organofosforados, carbamatos, triazinas e ureias por HPLC-MS/MS em urina de pessoas expostas em decorrência de atividades agrícolas é proposto neste trabalho. O modo de eluição isocrática e gradiente em cromatografia líquida de fase reversa com a coluna Synergi Max RP (150 x 4,6 mm, 4 &#181;m) foram experimentados para a separação dos componentes da amostra. O modo isocrático de eluição nas condições 45:55, 60:40 e 70:30 de ACN/água foram insuficientes para eluição dos componentes da amostra com fatores de retenção aceitáveis entre 0,5 e 2
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46

Machado, Marcos Canto. "Estudo e sistematização estatística e quimiométrica na determinação de hidrocarbonetos policíclicos aromáticos (HPAs) por HPLC-Flu /." Araraquara, 2012. http://hdl.handle.net/11449/97802.

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Orientador: Mary Rosa Rodrigues de Marchi<br>Coorientador: Marisa Veiga Capela<br>Banca: Eduardo Bessa Azevedo<br>Banca: Sandro José de Andrade<br>Resumo: A análise da literatura aponta para um crescente número de trabalhos em matrizes ambientais que buscam identificar e quantificar diversas substâncias poluentes. O estudo destas substâncias resulta em grandes conjuntos de dados, que necessitam ser devidamente analisados para a interpretação mais confiável possível. Por outro lado, os métodos analiticos utilizados nestas determinações envolvem diversas etapas e parâmetros que podem influenciar
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47

Machado, Marcos Canto [UNESP]. "Estudo e sistematização estatística e quimiométrica na determinação de hidrocarbonetos policíclicos aromáticos (HPAs) por HPLC-Flu." Universidade Estadual Paulista (UNESP), 2012. http://hdl.handle.net/11449/97802.

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Made available in DSpace on 2014-06-11T19:29:07Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-08-16Bitstream added on 2014-06-13T20:18:55Z : No. of bitstreams: 1 machado_mc_me_araiq.pdf: 1435306 bytes, checksum: 7ad8bbad80c831b71dec4d08b6da5555 (MD5)<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)<br>A análise da literatura aponta para um crescente número de trabalhos em matrizes ambientais que buscam identificar e quantificar diversas substâncias poluentes. O estudo destas substâncias resulta em grandes conjuntos de dados, que necessitam ser devidamente anali
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48

Alshana, Usama Ahmed. "Separation And Quantitation Of Some Platinum Group Metals By Rp-hplc." Master's thesis, METU, 2005. http://etd.lib.metu.edu.tr/upload/3/12605760/index.pdf.

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In this study, a reversed-phase high performance liquid chromatography (RP-HPLC) method has been developed to separate and determine Pt and Pd after formation of their chelates with N,N-diethyl-N&#039<br>-benzoylthiourea (DEBT). With the aim of reducing the number of steps in treating the samples, the method developed does not require the elimination of excess chelating reagent before the analysis of metal chelates. The different physical and chemical parameters affecting separation were examined in details. The whole analysis was completed on a C18 column in 16 min at 280 nm, with the mobile
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49

Perdue, James D. "The removal of Cremophor® EL from paclitaxel for quantitative analysis by HPLC-UV /." Electronic version (PDF), 2005. http://dl.uncw.edu/etd/2005/perduej/jamesperdue.pdf.

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50

Lindholm, Johan. "Development and Validation of HPLC Methods for Analytical and Preparative Purposes." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis, Univ.-bibl. [distributör], 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4442.

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