Journal articles: 'HPLC-ICP OES'

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Sch�ppenthau, J�rg, and Lothar Dunemann. "Hyphenated HPLC/ICP-MS and HPLC/ICP-OES techniques for the characterization of metal and non-metal species." Fresenius' Journal of Analytical Chemistry 349, no. 12 (1994): 794–99. http://dx.doi.org/10.1007/bf00323108.

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Amran, Mohammed B., Florence Lagarde, and Maurice J. F. Leroy. "Determination of arsenic species in marine organisms by HPLC-ICP-OES and HPLC-HG-QFAAS." Mikrochimica Acta 127, no. 3-4 (September 1997): 195–202. http://dx.doi.org/10.1007/bf01242722.

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Proch, Jędrzej, and Przemysław Niedzielski. "Iron species determination by high performance liquid chromatography with plasma based optical emission detectors: HPLC–MIP OES and HPLC–ICP OES." Talanta 231 (August 2021): 122403. http://dx.doi.org/10.1016/j.talanta.2021.122403.

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Fišera, Miroslav, Stanislav Kráčmar, Helena Velichová, Lenka Fišerová, Pavla Burešová, and Pavel Tvrzník. "Tin compounds in food – their distribution and determination." Potravinarstvo Slovak Journal of Food Sciences 13, no. 1 (May 28, 2019): 369–77. http://dx.doi.org/10.5219/1041.

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The aim of this work was optimization of the methods of trace- and ultratrace analysis, such as ICP-OES, ETA-AAS for charting the resources of individual forms of tin in foodstuffs. Increase of the sensitivity of the method of ICP-OES was achieved using the techniques of generation of hydrides, which was also optimized. Based on the information available on the occurrence of the different forms of tin, it appears that many of these organometallic compounds are contained in marine animals; attention has mainly focused on organisms such as marine fish, crustaceans, molluscs and algae. Tin compounds of predominantly inorganic origin can be found in foods and beverages which are packed in cans with a protective tin coating, too. The above mentioned methods have been applied to the analysis of selected beverages with low content of tin such as Coca Cola, Sprite, Fanta, Gambrinus 10°, PowerKing, and milk in the cans. Furthermore samples of animal origin as Sardines in oil, and Hunter's salami were examined, too. Prior to the determination of tin, samples need to be appropriately modified or analysed. Decomposition of the samples was done in the microwave system. Low pressure ion exchange chromatography with on-line detection of ICP-OES was used for separation of inorganic tin compounds. Separation of organically bound tin compounds was performed by HPLC on a column of ACE C-18, 3 µm, 15 cm × 1.0 mm with off-line detection by ETA-AAS. All of the above forms of tin compounds can be separated with this column. Due to the improvement in the detection of organically bounded tin, HPLC with identical ACE C-18 column coupled online for example with ICP-MS or spectrofluorimetry could be recommended.

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Holden, Marcia J., Savelas A. Rabb, Yadu B. Tewari, and Michael R. Winchester. "Traceable Phosphorus Measurements by ICP-OES and HPLC for the Quantitation of DNA." Analytical Chemistry 79, no. 4 (February 2007): 1536–41. http://dx.doi.org/10.1021/ac061463b.

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Grümping, R., and A. V. Hirner. "HPLC/ICP-OES determination of water-soluble silicone (PDMS) degradation products in leachates." Fresenius' Journal of Analytical Chemistry 363, no. 4 (February 17, 1999): 347–52. http://dx.doi.org/10.1007/s002160051201.

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Bodur, Süleyman, Sezin Erarpat, Utku Balçık, and Sezgin Bakırdere. "A rapid, sensitive and accurate determination of cobalamin with double monitoring system: HPLC-UV and HPLC-ICP-OES." Food Chemistry 340 (March 2021): 127945. http://dx.doi.org/10.1016/j.foodchem.2020.127945.

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Suvarapu, Lakshmi Narayana, and Sung-Ok Baek. "Recent Studies on the Speciation and Determination of Mercury in Different Environmental Matrices Using Various Analytical Techniques." International Journal of Analytical Chemistry 2017 (2017): 1–28. http://dx.doi.org/10.1155/2017/3624015.

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This paper reviews the current research on the speciation and determination of mercury by various analytical techniques, including the atomic absorption spectrometry (AAS), voltammetry, inductively coupled plasma optical emission spectrometry (ICP-OES), ICP-mass spectrometry (MS), atomic fluorescence spectrometry (AFS), spectrophotometry, spectrofluorometry, and high performance liquid chromatography (HPLC). Approximately 96 research papers on the speciation and determination of mercury by various analytical instruments published in international journals since 2015 were reviewed. All analytical parameters, including the limits of detection, linearity range, quality assurance and control, applicability, and interfering ions, evaluated in the reviewed articles were tabulated. In this review, we found a lack of information in speciation studies of mercury in recent years. Another important conclusion from this review was that there were few studies regarding the concentration of mercury in the atmosphere.

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Mateos-Maces, Lourdes, José Luis Chávez-Servia, Araceli Minerva Vera-Guzmán, Elia Nora Aquino-Bolaños, Jimena E. Alba-Jiménez, and Bethsabe Belem Villagómez-González. "Edible Leafy Plants from Mexico as Sources of Antioxidant Compounds, and Their Nutritional, Nutraceutical and Antimicrobial Potential: A Review." Antioxidants 9, no. 6 (June 20, 2020): 541. http://dx.doi.org/10.3390/antiox9060541.

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A review of indigenous Mexican plants with edible stems and leaves and their nutritional and nutraceutical potential was conducted, complemented by the authors’ experiences. In Mexico, more than 250 species with edible stems, leaves, vines and flowers, known as “quelites,” are collected or are cultivated and consumed. The assessment of the quelite composition depends on the chemical characteristics of the compounds being evaluated; the protein quality is a direct function of the amino acid content, which is evaluated by high-performance liquid chromatography (HPLC), and the contribution of minerals is evaluated by atomic absorption spectrometry, inductively coupled plasma-optical emission spectrometry (ICP-OES) or ICP mass spectrometry. The total contents of phenols, flavonoids, carotenoids, saponins and other general compounds have been analyzed using UV-vis spectrophotometry and by HPLC. For the determination of specific compounds such as phenolic compounds, flavonoids, organic acids and other profiles, it is recommended to use HPLC-DAD, UHPLC-DAD, UFLC-PDA or gas chromatography-mass spectrometry. The current biochemical analysis and biological evaluations were performed to understand the mechanisms of action that lead to decreased glucose levels and lipid peroxidation, increased hypoglycemic and antitumor activity, immune system improvement, increased antibacterial and antifungal activity and, in some cases, anti-Helicobacter pylori activity.

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Zvezdanović, Jelena, Sanja Petrović, Saša Savić, Dragan Cvetković, Ljiljana Stanojević, Jelena Stanojević, and Aleksandar Lazarević. "Phenolics and mineral content in St. John’s wort infusions from Serbia origin: An HPLC and ICP-OES study." Chemical Papers 75, no. 6 (February 2, 2021): 2807–17. http://dx.doi.org/10.1007/s11696-021-01521-1.

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Sysalova, Jirina, Jirina Szakova, Walter Goessler, and Jana Tremlova. "Methodological study of extraction procedures applied to urban particulate matter." Open Chemistry 9, no. 6 (December 1, 2011): 1071–79. http://dx.doi.org/10.2478/s11532-011-0097-4.

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AbstractThe modified BCR three-step sequential extraction procedure has been applied to two different samples of urban particulate matters (PM). The distribution of selected trace elements As, Cd, Cr, Mn, Ni, Pb, Zn was investigated and, in a comparative study, the presence of common organic air filters in extraction procedures was evaluated. Analytes in separate fractions were determined by ICP-OES and GFAAS, respectively, depending on concentration levels. While, due to air filters, a significant increase of some analytes mobility in individual fractions has been observed in case of the jet-milled PM (tunnel Letna), but in case of the PKC sample such effect was not found. The analyte impurities built in some filters has been tested, and the impact on the reliability of analyte results has been discussed. The arsenic species occurrence and their stability in presence of air filters (size 47 mm) were investigated in both urban PM samples as well, using HPLC-ICP-MS technique. Water soluble and by three-step BCR procedure extractable arsenic forms are shown in chromatograms

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Horacek, M., K. Kolar, M. Hola, B. Tobolkova, T. Vaculovic, C. Philipp, B. Marosanovic, et al. "Investigation of geographic origin of wine from border regions: Potential limitations and possibilities of different analytical methods and combinations of methods to identify the correct side of the border." BIO Web of Conferences 12 (2019): 02032. http://dx.doi.org/10.1051/bioconf/20191202032.

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In the presented project the investigation on discrimination of geographic origin of wine in from border areas is carried out. Authentic wine samples from the border areas of Austria, Czech Republic, Slovakia (and from Serbia) are investigated and compared by applying different physico-chemical methods (e.g. IRMS, NMR, ICP-MS, ICP-OES, EPR, HPLC, UV-VIS, etc.). The comparison of the data sets from the participating countries and the different applied methods will show, to which extent a differentiation can still be achieved in the border areas of the respective countries, which methods prove most efficient and sensitive and if a combination of methods will lead to an increased sensitivity. Furthermore, we also investigate the causes for the potential differentiation, which might be environmentally determined, due to different agricultural and/or enological practices. Results from the first investigated vintage 2016 show a good separation of the respective geographic origins by isotope ratios and element concentrations as well as by other parameters. The relevant parameters for differentiation now have to be critically evaluated to exclude potential lab influences.

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Paunoic, Dusan D., Snezana S. Mitic, Ivana D. Rasic Misic, Milan N. Mitic, Aleksandra N. Pavlovic, and Gordana M. Kocic. "Analyses of Metals Impact on Humulus lupulus Strobili Antioxidant Capacity." Revista de Chimie 71, no. 7 (August 4, 2020): 234–47. http://dx.doi.org/10.37358/rc.20.7.8241.

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This paper presents results of analyses of metal ions effects on hop strobili antioxidant characteristics. Determination of total phenols (TP), total flavonoids (TF) and fifteen phenolic compounds, as well as the antioxidant activity (DPPH, ABTS and FRAP) of hop extracts from eight samples was conducted using UV/Vis spectrophotometry and HPLC method. Contents of 24 elements in mineralized hop samples were determined by ICP-OES. Very strong negative relationship between TP, TF, antioxidant capacity results and Pb, Co, Cr, Sb and Na was determined applying principal component and cluster analyses. Namely, the higher concentrations of these metals were associated with lower contents of TP and TF and lower values of antioxidant tests. Impact of metal ions on phenolic and flavonoids content and antioxidant activity of hop strobili has not been previously published.

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Bonvehí, Josep Serra, and Rossend Escolá Jordà. "Constituents of Cocoa Husks." Zeitschrift für Naturforschung C 53, no. 9-10 (October 1, 1998): 785–92. http://dx.doi.org/10.1515/znc-1998-9-1002.

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Mineral composition, vitamins (group B, C and D), polyphenols, purine alkaloids, sugars and phytic acid were determined in twelve samples of cocoa husk of the main varieties of cocoa beans ( Theobroma cacao L.) cultivated in Africa and South America. Macro- (K, Mg, Ca, Na, P, and Na) and micro elements (Cu, Zn, Fe, and Cr) were quantified using an inductively coupled argon plasma-optical emission spectrometer (ICP-OES), on dry-ashed samples. Vitamins, phenolic compounds, alkaloids, sugars, and phytic acid were determined using HPLC. The mineral and vitamins intake that cocoa husk consumption could represent was evaluated with regard to the Recommended Dietary Allowances (RDA). The percentage of phytic acid is medium (0.98±0.13 g/100 g). The most abundant phenolic compounds found were caffeic acid and gentisic acid (2,5-dihydroxybenzoic acid).

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Freschi, Gian Paulo Giovanni. "Speciation of Different Forms of Se in Bovine Blood by Enzymatic Extraction and HPLC/Photo-Reduction/HG-ICP-OES." Atomic Spectroscopy 35, no. 5 (October 25, 2014): 205–12. http://dx.doi.org/10.46770/as.2014.05.004.

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Horacek, M., M. Hola, B. Tobolkova, K. Kolar, T. Vaculovic, O. Mikes, B. Marosanovic, et al. "Investigation of geographic origin of wine from border regions: Results from investigation of two vintages." BIO Web of Conferences 15 (2019): 02039. http://dx.doi.org/10.1051/bioconf/20191502039.

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Investigation on discrimination of geographic origin in border areas is carried out by investigating and comparing authentic wine samples from the border areas of Austria, Czech Republic, Slovakia (and from Serbia) by applying different physico-chemical methods (e.g. IRMS, NMR, ICP-MS, ICP-OES, EPR, HPLC, UV-VIS, etc…). The comparison of the data sets from the participating countries and the different applied methods demonstrates, to which extent a differentiation can still be achieved in the border areas of the respective countries, which methods prove most efficient and sensitive and if a combination of methods leads to an increased sensitivity. Furthermore, we also investigate the potential causes for the differentiation, as the environment, different agricultural practices or enology. Analysis of two consecutive vintages shows significant variations between the two years, complicating direct comparison of regions beyond individual vintages. Still, several parameters are identified enabling correct and complete differentiation. However, some of them have been found to represent lab effects, whereas others are interpreted as resulting from different agricultural practices or environmentally driven. Only the latter two ones, if consistent, can be used for discrimination of geographic origin.

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Alif, Matlal Fajri, Kozo Matsumoto, and Kuniyuki Kitagawa. "Analysis of S-containing compounds from the hydrothermal reaction of durian wastes by ICP–OES and HPLC–SEC/TOF-MS." Microchemical Journal 108 (May 2013): 68–73. http://dx.doi.org/10.1016/j.microc.2013.01.001.

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de Souza, Maiara P., Tiele M. Rizzetti, Michele Hoeltz, Mainara Dahmer, João A. Júnior, Gisele Alves, Lisianne B. Benitez, and Rosana C. S. Schneider. "Bioproducts characterization of residual periphytic biomass produced in an algal turf scrubber (ATS) bioremediation system." Water Science and Technology 82, no. 6 (July 27, 2020): 1247–59. http://dx.doi.org/10.2166/wst.2020.343.

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Abstract The transformation of residual biomass from bioremediation processes into new products is a worldwide trend driven by economic, environmental and social gain. The present study aimed to evaluate the potential for obtaining bioproducts of technological interest from the remaining periphytic biomass formed during a bioremediation process with an algal turf scrubber (ATS) system installed in a lake catchment. Different methodologies were used according to the target bioproduct. Analyses were performed by high performance liquid chromatography with diode array detector (HPLC/DAD), gas chromatography mass spectrometry (GC-MS), ultraviolet–visible spectroscopy (UV-VIS) and inductively coupled plasma optical emission spectrometry (ICP-OES). The results demonstrated that the periphytic biomass presented potential since protein (17.7%), carbohydrates (22.4%), total lipids (3.3%) with 3.6 mg mL−1 of fatty acids, antioxidants (144.5 μmol Trolox eq. g−1) and chlorophyll a, chlorophyll b and carotenoids (1,719.7 μg mL−1, 541.2 μg mL−1 and 317.7 μg mL−1, respectively) were obtained. Inorganic analysis presented a value of 42.3 ± 2.58% of total ash and metal presence was detected, indicating bioaccumulation. The properties found in periphyton strengthen the possibility of its application in different areas, ensuring bioremediation efficiency.

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Kostecka-Gugała, Anna, Michał Kruczek, Iwona Ledwożyw-Smoleń, and Paweł Kaszycki. "Antioxidants and Health-Beneficial Nutrients in Fruits of Eighteen Cucurbita Cultivars: Analysis of Diversity and Dietary Implications." Molecules 25, no. 8 (April 14, 2020): 1792. http://dx.doi.org/10.3390/molecules25081792.

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Aging is accompanied by gradual accumulation of molecular damage within cells in response to oxidative stress resulting from adverse environmental factors, inappropriate lifestyle, and numerous diseases. Adequate antioxidant intake is a key factor of proper diet. The study aimed to assess the antioxidant/antiradical capacities of Cucurbita fruits (18 cultivars of the species: C. maxima Duch., C. moschata Duch., C. pepo L., and C. ficifolia Bouché) grown in central Europe. The analyses were based on the FRAP (ferric reducing antioxidant power), CUPRAC (cupric ion reducing antioxidant capacity), and DPPH (2,2-diphenyl-1-picrylhydrazyl radical) assays. The content of phenolic compounds and β-carotene was evaluated with HPLC (high performance liquid chromatography), while the main macro- and micronutrients by ICP-OES (inductively coupled plasma mass spectrometry). The results revealed high intraspecies variability within the Cucurbita genus. The Japanese ‘Kogigu’ fruits were distinguished as extraordinary sources of phenolic compounds, including syringic and protocatechuic acids, catechin, and kaempferol. Another popular cultivar ‘Hokkaido’ exhibited the highest antioxidant and antiradical capacities. Most of the fruits proved to be rich sources of zinc and copper. The obtained data are discussed in the context of optimized nutrition of the elderly and suggest that Cucurbita fruits should become daily components of their diet.

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Gowele, Victoria, Joyce Kinabo, Theresia Jumbe, Carolyn Kirschmann, Jan Frank, and Wolfgang Stuetz. "Provitamin A Carotenoids, Tocopherols, Ascorbic Acid and Minerals in Indigenous Leafy Vegetables from Tanzania." Foods 8, no. 1 (January 19, 2019): 35. http://dx.doi.org/10.3390/foods8010035.

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The essential micronutrients in indigenous leafy vegetables (ILVs) could substantially contribute to the micronutrient supply in rural communities in Tanzania, but concentrations differ between species. Provitamin A carotenoids, tocopherols, ascorbic acid, minerals, and phytate were analysed in 13 different species using HPLC-, ICP-OES, and photometric techniques. Eight of the 13 ILVs, including Amaranthus ssp. and Sesamum angustifolium, had high β-carotene concentrations (2.91–4.84 mg/100 g fresh weight), which could provide ≥50% of vitamin A’s recommended nutrient intake (RNI). Six ILVs including Cleome hirta and Sonchus luxurians had high iron contents (34.5–60.4 mg/100 g, >50% RNI); Amaranthus ssp. represented the ILV with high calcium, magnesium and zinc contents (85%, 207% and 21% of RNI per 100 g); Cleome hirta and Cleome gynandra had high ascorbic acid contents (>15 mg/100 g, 34–35% of RNI), while Sesamum angustifolium was the only ILV with a high tocopherol content (7.34 mg α-TE/100 g). The highest phytate concentration was found in Amaranthus ssp., which could negatively affect its role as a very good source of minerals. Results indicate that the analysed ILVs could make a substantial contribution to the vitamin A and iron supply in the diets of rural Tanzanian populations.

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Papapetros, Spyridon, Artemis Louppis, Ioanna Kosma, Stavros Kontakos, Anastasia Badeka, Chara Papastephanou, and Michael G. Kontominas. "Physicochemical, Spectroscopic and Chromatographic Analyses in Combination with Chemometrics for the Discrimination of Four Sweet Cherry Cultivars Grown in Northern Greece." Foods 8, no. 10 (September 26, 2019): 442. http://dx.doi.org/10.3390/foods8100442.

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A total of 56 sweet cherry samples belonging to four cultivars (Ferrovia, Canada Giant, Lapins, and Germersdorfer) grown in northern Greece were characterized and differentiated according to botanical origin. For the above purpose, the following parameters were determined: conventional quality parameters (titratable acidity (TA), pH, total soluble solids (TSS), total phenolic content (TPC), mechanical properties and sensory evaluation, sugars by High Performance Liquid Chromatography (HPLC), volatile compounds by GC/MS, and minerals by ICP-OES. Statistical treatment of the data was carried out using Multivariate Analysis of Variance (MANOVA) and Linear Discriminant Analysis (LDA). The results showed that the combination of volatile compounds and conventional quality parameters provided a correct classification rate of 84.1%, the combination of minerals and conventional quality parameters 86.4%, and the combination of minerals, conventional quality parameters and sugars provided the highest correct classification rate of 88.6%. When the above four cherry cultivars were combined with previously studied Kordia, Regina, Skeena and Mpakirtzeika cultivars, collected from the same regions during the same seasons, the respective values for the differentiation of all eight cultivars were: 85.5% for the combination of conventional quality parameters, volatiles and minerals; and 91.3% for the combination of conventional quality parameters, volatiles, minerals, and sugars.

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Silva, Alessandra Tesch da, Ewelyne Miranda de Lima, Isabela Faco Caliman, Leonardo Luiz Souza Porto, Andrews Marques do Nascimento, Iêda Carneiro Kalil, Dominik Lenz, Nazaré Souza Bissoli, Denise Coutinho Endringer, and Tadeu Uggere de Andrade. "Hypotensive effect and endothelium-dependent vascular action of leaves of Alpinia purpurata (Vieill) K. Schum." Brazilian Journal of Pharmaceutical Sciences 50, no. 2 (April 2014): 309–20. http://dx.doi.org/10.1590/s1984-82502014000200010.

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The aims of this study were to evaluate the chemical profile, vascular reactivity, and acute hypotensive effect (AHE) of the ethanolic extract of leaves of Alpinia purpurata (Vieill) K. Schum (EEAP). Its chemical profile was evaluated using HPLC-UV, ICP-OES, and colorimetric quantification of total flavonoids and polyphenols. The vascular reactivity of the extract was determined using the mesenteric bed isolated from WKY. AHE dose-response curves were obtained for both EEAP and inorganic material isolated from AP (IAP) in WKY and SHR animals. Cytotoxic and mutagenic safety levels were determined by the micronucleus test. Rutin-like flavonoids were quantified in the EEAP (1.8 ± 0.03%), and the total flavonoid and polyphenol ratios were 4.1 ± 1.8% and 5.1 ± 0.3%, respectively. We observed that the vasodilation action of EEAP was partially mediated by nitric oxide (·NO). The IAP showed the presence of calcium (137.76 ± 4.08 μg mg-1). The EEAP and IAP showed an AHE in WKY and SHR animals. EEAP did not have cytotoxic effects or cause chromosomic alterations. The AHE shown by EEAP could result from its endothelium-dependent vascular action. Rutin-like flavonoids, among other polyphenols, could contribute to these biological activities, and the calcium present in EEAP could act in a synergistic way.

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van Rayne, Kiana Kirsty, Oluwafemi Ayodeji Adebo, and Nomali Ziphorah Ngobese. "Nutritional and Physicochemical Characterization of Strychnos madagascariensis Poir (Black Monkey Orange) Seeds as a Potential Food Source." Foods 9, no. 8 (August 5, 2020): 1060. http://dx.doi.org/10.3390/foods9081060.

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Strychnos madagascariensis Poir is an underutilized fruit that is considered a valuable food during droughts and famine. The aim of this research was to characterize the nutritional composition and the flour functional properties, for the use as a potential food source. Seed flour was analysed using a standard enzymatic assay for sugars, acid/neutral detergent analysis for fibre, ether extraction for fat and HPLC for strychnine. Results showed that the seeds contained 41% reducing sugars and 53% fibre. The mineral composition, determined using microwave-assisted acid digestion and inductively coupled plasma optical emission spectrometry (ICP–OES), showed that the seeds contained high quantities of iron (15.78 mg/100 g) and manganese (9.86 mg/100 g). The flour water absorption index (1.37 g/g) was substantially higher than that of wheat, brown rice and tapioca flours and the oil absorption index showed similarities to the reference flours (1.09 g/g). The flour peak (37,788 RVU) and final viscosities (62,928 RVU) were significantly (p < 0.001) higher than the reference flours. This study was the first to quantify the strychnine content (0.08%) in the seeds. Results suggest that the seeds have good potential for food product development; however, further processing is essential to ensure safety for consumption.

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Konieczynski, Pawel, Aleksej Zarkov, Agnieszka Viapiana, Mateusz Kaszuba, Lukasz Bielski, and Marek Wesolowski. "Investigations of metallic elements and phenolics in Chinese medicinal plants." Open Chemistry 18, no. 1 (November 27, 2020): 1381–90. http://dx.doi.org/10.1515/chem-2020-0130.

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AbstractTraditional Chinese Medicines (TCM) can be contaminated with heavy metals, and therefore, the aim of this study is to analyze the Fe, Mn, Zn, Cu, Cd, Pb, Cr, and phenolic compounds contents in TCM plants used against civilization diseases. Metals were determined by flame atomic absorption spectroscopy (FAAS) for Fe, Mn, Zn, and Cu and inductively coupled plasma-optical emission spectroscopy (ICP-OES) for Pb, Cd, and Cr. The total phenolic, flavonoid, and phenolic acid contents were determined by HPLC and UV/vis spectrometry. The contents of the studied elements were highest in Radix Rehmanniae, whereas lowest in Fructus Lycii and Fructus Crataegi. The studied metals were assayed in the decreasing order: Fe, Zn, Mn, Cu, Cr, Pb, and Cd. Radix Rehmanniae Glutinosae Preparata showed the lowest phenolic composition, while Fructus Lycii showed the richest content. Principal component analysis (PCA) revealed that the contents of ferulic acid, caffeic acid, rutin, and Cu, Cr, and Cd were among the most important factors responsible for the differentiation between the investigated medicinal plants. Cluster analysis (CA) showed that the TCM samples originating from the same botanical plant species were often found in the same cluster, which confirms the similar level of studied elements determined within the samples.

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Ozkaya, Ahmet, Harun Ciftci, Okkes Yilmaz, Ahmet Zafer Tel, Erol Cil, and B. Sitki Cevrimli. "Vitamin, Trace Element, and Fatty Acid Levels ofVitex agnus-castusL.,Juniperus oxycedrusL., andPapaver somniferumL. Plant Seeds." Journal of Chemistry 2013 (2013): 1–4. http://dx.doi.org/10.1155/2013/845743.

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The levels of fat-soluble vitamin, trace element and fatty acid ofVitex agnus-castusL.,Juniperus oxycedrusL., andPapaver somniferumL. seeds in Turkey were determined by using HPLC, ICP-OES, and GC, respectively. In theVitex agnus-castusL.,Juniperus oxycedrusL., andPapaver somniferumL. seeds, linoleic acid (18 : 2) was determined with the highest level rates (%54.11, %28.03, and %72.14, resp.). In theVitex agnus-castusL. seeds, R-tocopherol,α-tocopherol, and K1levels were determined as 9.70 μg/g, 18.20 μg/g, and 24.79 μg/g, respectively; In theJuniperus oxycedrusL. seeds, R-tocopherol,α-tocopherol, and K1were determined as 18.50 μg/g, 0.84 μg/g, and 5.00 μg/g, respectively, and in thePapaver somniferumL. seeds, R-tocopherol,α-tocopherol, K1, and D2levels were determined as 43.25 μg/g, 122.05 μg/g, 12.01 μg/g, and 0.62 μg/g, respectively. In theVitex agnus-castusL.,Juniperus oxycedrusL., andPapaver somniferumL. seeds, nickel (Ni), zinc (Zn), and iron (Fe) were determined with the trace element level rates (4.42 mg/kg, 10.43 mg/kg, 3.71 mg/kg for Ni, 7.00 mg/kg, 7.70 mg/kg, and 24 mg/kg for Zn and 93.73 mg/kg, 187.95 mg/kg, and 149.64 mg/kg for Fe, resp.). These parameters in seeds are very important for human life.

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Laosuwan, Melasinee, Siriboon Mukdasai, and Supalax Srijaranai. "A Simple in Syringe Low Density Solvent-Dispersive Liquid Liquid Microextraction for Enrichment of Some Metal Ions Prior to Their Determination by High Performance Liquid Chromatography in Food Samples." Molecules 25, no. 3 (January 28, 2020): 552. http://dx.doi.org/10.3390/molecules25030552.

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A simple and highly sensitive method is developed for the simultaneous determination of Ni2+, Cr2O72−, Co2+, and Hg2+ by using in syringe low density solvent-dispersive liquid liquid microextraction (ISLD-DLLME), followed by high performance liquid chromatography with a UV detector. The four metal ions were derivatized with pyrrolidine dithiocarbamate (PDC) based on complexation before their enrichment by ISLD-DLLME in which 1-octanol and methanol were used as the extraction solvent and the dispersive solvent, respectively. The extraction was performed in a commercially available syringe under vortex agitation. Phase separation was achieved without centrifugation, and the extraction phase was easily collected by moving the syringe plunger. Parameters affecting the extraction efficiency were studied and optimized. Under the optimum conditions, the four metal-PDC complexes were detected within 18 min, and ISLD-DLLME could increase the detection sensitivity in the range of 64–230 times compared to the direct HPLC analysis. The obtained limits of detection (LODs) were found to be in the range of 0.011–2.0 µg L−1. The applicability of the method is demonstrated for freshwater fish, shrimp, and shellfish samples. In addition, the results are in good agreement with those obtained by inductively-coupled plasma-optical emission spectrometry (ICP-OES).

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Proch, Jędrzej, and Przemysław Niedzielski. "Multi–mode Sample Introduction System (MSIS) as an interface in the hyphenated system 2 HPLC–MSIS–ICP–OES in simultaneous determination of metals and metalloids species." Analytica Chimica Acta 1147 (February 2021): 1–14. http://dx.doi.org/10.1016/j.aca.2020.12.047.

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TODEA, Doina Adina, Oana CADAR, Dorina SIMEDRU, Cecilia ROMAN, Claudiu TANASELIA, Iuliu SUATEAN, and Anca NAGHIU. "Determination of Major-to-Trace Minerals and Polyphenols in Different Apple Cultivars." Notulae Botanicae Horti Agrobotanici Cluj-Napoca 42, no. 2 (December 2, 2014): 523–29. http://dx.doi.org/10.15835/nbha4229715.

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The aim of this study was to investigate the concentration level of some major-to-trace elements and toxic minerals and polyphenols (catechin, epicatechin and quercetin) in fruits belonging to different apple cultivars (â€˜Auriu de Clujâ€™, â€˜Florinaâ€™, â€˜Generosâ€™, â€˜Golden Deliciousâ€™, â€˜Primaâ€™, â€˜Productiv de Clujâ€™ and â€˜Starkrimsonâ€™), under specific climate conditions to northwestern Romania. The apple minerals were determined by the inductively coupled plasma mass spectrometry (ICP-MS) and inductively coupled plasma optical emission spectrometry (ICP-OES) after a preliminary, microwave-assisted, acid digestion. The polyphenols were determined by high performance liquid chromatography (HPLC) with MS/MS detection. According to the obtained results, the edible part of the apple contains many minerals with high relative nutritional value (RNV). The major minerals (Na, K, Ca, Mg and P), minor elements (Cu, Fe, Mn, Sr and Zn) and toxic trace elements (Cd, Cr and Pb) were comparatively identified and quantified. It was found that K (31976 mg/kg) was predominant among the major elements, while Fe (3.68 mg/kg) and Cu (3.57 mg/kg) were comparable. The toxic trace metals (Cd, Cr and Pb) were below the limits of detection (0.01, 0.10 and 0.01 mg/kg, respectively) and did not determine any health risk to consumers. The main polyphenols composition (catechin, epicathechin, quercetin) differs slightly among different cultivars. In â€˜Idaredâ€™ and â€˜Jonathanâ€™ cultivars, the investigated polyphenols are in higher quantities comparing to the others. In all investigated apple cultivars, quercetin was proved to be the major polyphenol, followed by epicathechin and catechin. These results suggest that, regarding only the investigated polyphenols, â€˜Idaredâ€™ and â€˜Jonathanâ€™ cultivars have the highest antioxidant character in all investigated genotypes.

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Pan, Meng, Wenxuan Pei, Yixin Yao, Ling Dong, and Jianbo Chen. "Rapid and Integrated Quality Assessment of Organic-Inorganic Composite Herbs by FTIR Spectroscopy—Global Chemical Fingerprints Identification and Multiple Marker Components Quantification of Indigo Naturalis (Qing Dai)." Molecules 23, no. 11 (October 24, 2018): 2743. http://dx.doi.org/10.3390/molecules23112743.

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This research aimed to develop an FTIR-based method for rapid and low-cost integrated quality assessment of organic-inorganic composite herbs, which are kinds of herbs composed of both organic and inorganic active ingredients or matrix components. A two-step quality assessment route was designed and verified using the example of Indigo Naturalis (Qing Dai). First, the FTIR spectra were used as global chemical fingerprints to identify the true and fake samples. Next, the contents of the organic and inorganic marker components were estimated by FTIR quantification models to assess the quality of the true samples. Using the above approaches, all the 56 true samples and five fake samples of Indigo Naturalis could be identified correctly by the correlation threshold of the FTIR chemical fingerprints. Furthermore, the FTIR calibration models provided an accurate estimation of the contents of marker components with respect to HPLC and inductively coupled plasma optical emission spectrometry (ICP-OES). The coefficients of determination (R2) for the independent validation of indigo, indirubin, and calcium were 0.977, 0.983, and 0.971, respectively. Meanwhile, the mean relative errors (MRE) for the independent validation of indigo, indirubin, and calcium were 2.2%, 2.4%, and 1.8%, respectively. In conclusion, this research shows the potential of FTIR spectroscopy for the rapid and integrated quality assessment of organic-inorganic composite herbs in both chemical fingerprints identification and marker components quantification.

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Irwan, Wawan. "Development of High Betacarotene Instant Cream of Pumpkin (Cucurbita moschata) Soup for the Elderly." Current Developments in Nutrition 4, Supplement_2 (May 29, 2020): 35. http://dx.doi.org/10.1093/cdn/nzaa040_035.

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Abstract Objectives The aim of this study was to develop instant cream of pumpkin (Cucurbita moschata) soup high in b-carotene for the elderly. Methods The design was a completely randomized factorial design with two replications and three formulas. There were two factors tested including the type of processing (fresh vs instant) and the proportion of pumpkin and carrot (1:2 vs 1:1 vs 2:1). The formulas were evaluated by semi-trained panellists for the hedonic test, while by the elderly for the acceptance tests. The routine proximate analysis was then conducted. In addition, the potassium and b-carotene contents were also determined by inductively couple plasma–optical emission spectrophotometer (ICP-OES) and high performance liquid chromatography (HPLC), respectively. Results The selected formula was soup processed instantly with proportion of pumpkin and carrot of 2:1. The elderly preferred instant soup to the fresh. The selected formula contained 491.69 kcal/100 g, water (3.87%), ash (2.22%), protein (9.82%), fat (23.21%) and carbohydrate (60.88%). In addition, the potassium and b-carotene content of selected formula were 501.35 mg/100 g and 3380 μg/g, respectively. In addition, the selected formula met 100% of RDA per serving size. Conclusions The elderly preferred instant cream of pumpkin soup to the fresh with pumpkin and carrot ratio of 2:1. The instant cream of pumpkin soup is a nutritious food high in b-carotene. Funding Sources Centre For Development and Empowerment teacher and education personnel, Ministry Education and Culture, Republic of Indonesia.

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Arshad, Mehreen, Yumna Sadef, Muhammad Bilal Shakoor, Muhammad Naeem, Farzana Bashir, Sajid Rashid Ahmad, Shafaqat Ali, Irfan Abid, Naeem Khan, and Mohammed Nasser Alyemeni. "Quantitative Estimation of the Hydroquinone, Mercury and Total Plate Count in Skin-Lightening Creams." Sustainability 13, no. 16 (August 6, 2021): 8786. http://dx.doi.org/10.3390/su13168786.

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Generally white color of the skin is regarded as a feature of superiority and prettiness around the world. Both the males and females in Pakistan apply skin-lightening creams (SLC) but they do not know about the side-effects of their constituents. Skin-lightening products include SLC and related ointments. The SLC are made by mixing fates and water in standard procedure. Here, 20 SLC specimens were obtained and subjected to mercury, hydroquinone and the total plate count (TPC). The hydroquinone in SLC was determined using HPLC, mercury level was assessed by ICP OES and finally TPC were computing by utilizing nutrient media (Agar). The hydroquinone in SLC ranged from 0 to 7.14 ± 0.18% with a median value of 0.33%. In 25% of the studied samples, hydroquinone was not detected, 70% of the samples showed values within the limit and 5% of the samples (1 sample) had a hydroquinone concentration above the permissible limit defined by Pakistan (5%). The mercury ranged 0-7.7 ppm, with a median value of 2.5 ppm. Mercury was detected in 95% of the samples; thus, only 5% of the samples had no mercury. In turn, 20% had mercury within the limit value while 75% of the samples had concentration above the Pakistan standard limit (1 ppm). Moreover, TPC obtained in this study was less than the allowable value set according to European Union (EU). Hence, the SLC samples showed high concentration of toxic constituents which could cause deleterious skin diseases. Government must monitor such kind of cosmetic products regularly in order to reduce the danger.

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Lachowicz, Sabina, Jan Oszmiański, Martyna Wilczyńska, Grzegorz Zaguła, Bogdan Saletnik, and Czesław Puchalski. "Impact Mineralization of Chokeberry and Cranberry Fruit Juices Using a New Functional Additive on the Protection of Bioactive Compounds and Antioxidative Properties." Molecules 25, no. 3 (February 4, 2020): 659. http://dx.doi.org/10.3390/molecules25030659.

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Chicken eggshells can be used as an attractive dietary source of mineral compounds, including calcium (Ca). However, the effects of chicken eggshell powder (CESP) on berry fruit juices have not been studied to date. Therefore, the objective of this study was to evaluate the effect of its addition to juices from chokeberry and cranberry on their phytochemical properties. The juices were determined for contents of polyphenols (determined by ultra-efficient liquid chromatography coupled with a mass detector (UPLC-PDA-ESI-MS/MS)), macro- and microelements (by inductively coupled plasma - optical emission spectrometry (ICP-OES)), and organic acids (by high-performance liquid chromatography (HPLC-PDA)) as well as for their antioxidative activity by radical scavenging capacity (ABTS) and ferric reducing antioxidative power (FRAP) assay, color profile (CIE L* a* b* system), and sensory attributes. The study results demonstrate that CESP addition to chokeberry and cranberry juices enriched them with minerals and increased their Ca content 25.7 times and 66.3 times, respectively, compared to the control samples. Juices supplementation with CESP significantly decreased their acidity and total organic acids content as well as increased their pH value. Chokeberry and cranberry juices supplementation with 1% CESP caused no significant changes in the amount of precipitate and their color, but it significantly improved their taste. For this reason, CESP addition in the amount of up to 1% can be suggested as the optimal supplementation of berry fruit juices. The study also demonstrated that CESP addition in the amount of up to 1% caused no significant differences in the content of polyphenolic compounds and in the antioxidative activity of juices, which can be deemed important from the viewpoint of their putative health benefits. In addition, the heat treatment of juices contributed to only a 4% loss of polyphenolic compounds from the CESP-supplemented juices compared to the 6% loss from the non-supplemented juices.

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Kyriacou, Marios C., Christophe El-Nakhel, Antonio Pannico, Giulia Graziani, Armando Zarrelli, Georgios A. Soteriou, Angelos Kyratzis, et al. "Ontogenetic Variation in the Mineral, Phytochemical and Yield Attributes of Brassicaceous Microgreens." Foods 10, no. 5 (May 10, 2021): 1032. http://dx.doi.org/10.3390/foods10051032.

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Microgreens constitute novel gastronomic ingredients that combine visual, kinesthetic and bioactive qualities. The definition of the optimal developmental stage for harvesting microgreens remains fluid. Their superior phytochemical content against mature leaves underpins the current hypothesis of significant changes in compositional profile during the brief interval of ontogeny from the appearance of the first (S1) to the second true leaf (S2). Microgreens of four brassicaceous genotypes (Komatsuna, Mibuna, Mizuna and Pak Choi) grown under controlled conditions and harvested at S1 and S2 were appraised for fresh and dry yield traits. They were further analyzed for macro- and micromineral content using inductively coupled plasma optical emission spectrometry (ICP-OES), carotenoid content using high-performance liquid chromatography with a diode-array detector (HPLC-DAD), volatile organic compounds using solid-phase microextraction followed by gas chromatography-mass spectrometry (SPME-GC/MS), anthocyanins and polyphenols using liquid chromatography-high resolution-tandem mass spectrometry (LC-MS/MS) with Orbitrap technology and for chlorophyll and ascorbate concentrations, well as antioxidant capacity by spectrophotometry. Analysis of compositional profiles revealed genotype as the principal source of variation for all constituents. The response of mineral and phytochemical composition and of antioxidant capacity to the growth stage was limited and largely genotype-dependent. It is, therefore, questionable whether delaying harvest from S1 to S2 would significantly improve the bioactive value of microgreens while the cost-benefit analysis for this decision must be genotype-specific. Finally, the lower-yielding genotypes (Mizuna and Pak Choi) registered higher relative increase in fresh yield between S1 and S2, compared to the faster-growing and higher-yielding genotypes. Although the optimal harvest stage for specific genotypes must be determined considering the increase in yield against reduction in crop turnover, harvesting at S2 seems advisable for the lower-yielding genotypes.

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van Wyk, Pieter-Hugo, Wilhelmus J. Gerber, and Klaus R. Koch. "Direct determination of metal to halide mole ratios in platinum complex anions [PtCl6−nBrn]2− (n = 0–6) by means of HPLC-ICP-OES using Cl, Br and Pt emissions of all separated species." Journal of Analytical Atomic Spectrometry 27, no. 4 (2012): 577. http://dx.doi.org/10.1039/c2ja10342g.

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Carpio R., Rossemary V., Alejandrina Sotelo M., and Wolfgang J. Grüneberg. "Contenido de a-Caroteno, Hierro y Zinc, Efecto de Almacenamiento y Tipo de Cocción en Genotipos de Camote (Ipomoea batatas L)." Revista de Investigaciones Veterinarias del Perú 28, no. 2 (July 23, 2017): 242. http://dx.doi.org/10.15381/rivep.v28i2.13076.

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El camote (Ipomoea batatas L) es el principal componente en la dieta de millones de personas que viven en países tropicales, y un mejoramiento en el contenido de â-caroteno podría incrementar el suministro de vitamina A para satisfacer la mayor parte de las necesidades diarias de la gente. En el presente estudio, el contenido de â-caroteno (BC), hierro (Fe) y zinc (Zn) fueron determinados en 25 genotipos de camote provenientes de las localidades de La Molina y San Ramón (Perú). Así mismo, los cambios en el contenido de BC en siete genotipos de camote fueron investigados usando diferentes métodos de cocción y tiempos de almacenamiento. El contenido de BC fue medido vía absorbancia a 450 nm e identificado a través del HPLC y el contenido de Fe y Zn fueron determinados a través de técnicas de ICP-OES. Los resultados muestran variación significativa entre genotipo, ambiente y en la interacción genotipo-ambiente. La concentración de BC en las raíces de camote recién cosechado varió entre 7.62 y 18.93 mg/100 g en peso fresco (PF). El contenido de hierro presentó un rango de 0.40 – 0.96 mg/100 g PF y el contenido de zinc presentó un rango de 0.25 – 0.51 mg/100 mg PF. Los genotipos 440442 y 440518 presentaron los niveles más altos de BC en los dos ambientes. Diferencias en el contenido de BC fueron encontrados entre los métodos de cocción y los tiempos de almacenamiento. La concentración de BC de las raíces cocidas por ebullición (15.15 mg/100 g PF) fue mayor que las raíces cocidas por horneado (14.29 mg/100 g PF); sin embargo, no hubo diferencias entre los genotipos 440442 y 420081 por efecto del método de cocción. Asimismo, el contenido de BC se incrementó durante el tiempo de almacenamiento, donde el genotipo 440442 presentó los mayores niveles de BC durante el tiempo de almacenamiento, mientras que los genotipos 440413 y 440513 no presentaron cambios significativos.

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Pannico, Antonio, Christophe El-Nakhel, Giulia Graziani, Marios C. Kyriacou, Maria Giordano, Georgios A. Soteriou, Armando Zarrelli, Alberto Ritieni, Stefania De Pascale, and Youssef Rouphael. "Selenium Biofortification Impacts the Nutritive Value, Polyphenolic Content, and Bioactive Constitution of Variable Microgreens Genotypes." Antioxidants 9, no. 4 (March 25, 2020): 272. http://dx.doi.org/10.3390/antiox9040272.

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Selenium (Se) is considered essential for human nutrition as it is involved in the metabolic pathway of selenoproteins and relevant biological functions. Microgreens, defined as tender immature greens, constitute an emerging functional food characterized by overall higher levels of phytonutrients than their mature counterparts. The nutraceutical value of microgreens can be further improved through Se biofortification, delivering Se-enriched foods and potentially an enhanced content of bioactive compounds. The current study defined the effect of sodium selenate applications at three concentrations (0, 8, and 16 μM Se) on the bioactive compounds and mineral content of coriander, green basil, purple basil, and tatsoi microgreens grown in soilless cultivation. Analytical emphasis was dedicated to the identification and quantification of polyphenols by UHPLC-Q-Orbitrap-HRMS, major carotenoids by HPLC-DAD, and macro micro-minerals by ICP-OES. Twenty-seven phenolic compounds were quantified, of which the most abundant were: Chlorogenic acid and rutin in coriander, caffeic acid hexoside and kaempferol-3-O(caffeoyl) sophoroside-7-O-glucoside in tatsoi, and cichoric acid and rosmarinic acid in both green and purple basil. In coriander and tatsoi microgreens, the application of 16 μM Se increased the total phenols content by 21% and 95%, respectively; moreover, it improved the yield by 44% and 18%, respectively. At the same Se dose, the bioactive value of coriander and tatsoi was enhanced by a significant increase in rutin (33%) and kaempferol-3-O(feruloyl)sophoroside-7-O-glucoside (157%), respectively, compared to the control. In green and purple basil microgreens, the 8 μM Se application enhanced the lutein concentration by 7% and 19%, respectively. The same application rate also increased the overall macroelements content by 35% and total polyphenols concentration by 32% but only in the green cultivar. The latter actually had a tripled chicoric acid content compared to the untreated control. All microgreen genotypes exhibited an increase in the Se content in response to the biofortification treatments, thereby satisfying the recommended daily allowance for Se (RDA-Se) from 20% to 133%. The optimal Se dose that guarantees the effectiveness of Se biofortification and improves the content of bioactive compounds was 16 μM in coriander and tatsoi, and 8 μM in green and purple basil.

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Proch, Jędrzej, and Przemysław Niedzielski. "In–spray chamber hydride generation by multi–mode sample introduction system (MSIS) as an interface in the hyphenated system of high performance liquid chromatography and inductivity coupled plasma optical emission spectrometry (HPLC/HG–ICP–OES) in arsenic species determination." Talanta 208 (February 2020): 120395. http://dx.doi.org/10.1016/j.talanta.2019.120395.

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Merdzhanova, A., V. Panayotova, D. A. Dobreva, and K. Peycheva. "Proximate composition, lipid quality and heavy metals content in the muscle of two carp species." Agricultural Science and Technology 10, no. 4 (2018): 358–69. http://dx.doi.org/10.15547/10.15547/ast.2018.04.066.

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Abstract. The aim of the presented study was to characterize the quality of edible tissue of freshwater common carp (Cyprinus carpio) and bighead carp (Aristichthys nobilis), based on their proximate and lipid composition (lipid classes, fatty acid profile, fat soluble vitamins, carotenoids and cholesterol). Health risk assessment was evaluated based on the analysis of some toxic elements (As, Cd, Ni, Pb and total Hg). Proximate composition (moisture, crude protein and total lipid) was determined using standard procedures. Lipids were subsequently separated into neutral (NL) and polar lipids: Phospho- (PL) and Glycolipids (GL) by means of column and thin-layer chromatography. Lipid classes were derivatized into fatty acid methyl esters (FAMEs) which were analysed by gas chromatography–mass spectrometry (GC-MS). Vitamins A, D3 and E, beta-carotene, astaxanthin and cholesterol were analysed simultaneously using high performance liquid chromatography (HPLC). Heavy metals (As, Pb, Cd, Hg and Ni) were determined by optical emission spectrometry with inductively coupled plasma (ICP-OES) following a microwave digestion procedure. Protein content was higher in bighead carp (18.5%) and lower for common carp (15.5%), whereas lipid content showed opposite trend. Similarities in lipid classes distribution were observed for both species: NL>GL>PL. Neutral lipids constituted approximately 70% of TL in both species, as FAs profile was dominated by monounsaturated fatty acids (MUFA), whereas polyunsaturated FAs (PUFA) prevailed in polar fractions. Omega-3 PUFAs were higher in all lipid classes compared to omega-6 PUFAs. Cholesterol content was low (17-24 mg.100-1g ww). Astaxanthin was detected only in bighead carp, whereas beta-carotene, vitamin D3 and vitamin A showed similar concentrations in both samples. Vitamin E content was higher in bighead carp (10.4 mg.100 g-1 w.w.). Trace elements content was higher in bighead carp showing a maximum value of As (0.312 mg.kg-1 w.w). All determined toxic elements were found below the recommended value in carp muscle. The results of the present study confirmed the high quality and safety of common carp and bighead carp meat. These freshwater species are valuable sources of essential nutrients such as proteins, vitamin D3 and long chain omega-3 PUFAs. Together with the nutrients, the information for low concentrations of toxic elements makes them valuable components of a healthy human diet.

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van Wyk, Pieter-Hugo, Wilhelmus J. Gerber, and Klaus R. Koch. "A robust method for speciation, separation and photometric characterization of all [PtCl6−nBrn]2− (n=0–6) and [PtCl4−nBrn]2− (n=0–4) complex anions by means of ion-pairing RP-HPLC coupled to ICP-MS/OES, validated by high resolution 195Pt NMR spectroscopy." Analytica Chimica Acta 704, no. 1-2 (October 2011): 154–61. http://dx.doi.org/10.1016/j.aca.2011.07.037.

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Haraguchi, Hiroki, Atsushi Ishii, Takuya Hasegawa, Hirotaka Matsuura, and Tomonari Umemura. "Metallomics study on all-elements analysis of salmon egg cells and fractionation analysis of metals in cell cytoplasm." Pure and Applied Chemistry 80, no. 12 (January 1, 2008): 2595–608. http://dx.doi.org/10.1351/pac200880122595.

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Salmon egg cells were the subject of investigation for all-elements analysis in a single biological cell, where the elements in egg cells were simultaneously determined by inductively coupled plasma atomic emission spectrometry (ICP-AES) and inductively coupled plasma mass spectrometry (ICP-MS) after microwave-assisted acid digestion. As a result, 74 elements among 78 stable isotope elements were determined or detected, although H, C, N, and O were determined separately by conventional CHN elemental analysis. In addition, the survey of protein-binding metallic elements as well as metalloid elements in salmon egg cell cytoplasm was performed by the fractionation analysis with a hyphenated system of high-performance liquid chromatography (HPLC)/ICP-MS using a 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane sulfonate (CHAPS)-coated octadecylsilyl silica (ODS) column and a size exclusion chromatography (SEC) column.

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Heltai, G., B. Fehér, and M. Horváth. "Coupling of microwave induced plasma optical emission spectrometry with HPLC separation for speciation analysis of Cr(III)/Cr(VI)." Chemical Papers 61, no. 6 (January 1, 2007). http://dx.doi.org/10.2478/s11696-007-0059-7.

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AbstractFeasibility and limitations of direct coupling of high performance liquid chromatographic (HPLC) separation to microwave induced plasma (MIP)-optical emission spectrometry (OES) for elementspecific detection was tested and compared to inductively coupled plasma (ICP)-optical emission spectrometric detection on the basis of the Cr(III)/Cr(VI) speciation analysis of water samples. Coupling was performed by a hydraulic high pressure nebulizer (HHPN) radiative-heating/watercooling interface which provides about 20 % and 80 % aerosol yield in the case of helium and argon carrier gases, respectively. Desolvation efficiency of aqueous solutions was approximately 80 %. Applying the ion-pair HPLC separation, the organic eluents and reagents in the MIP cause a 50–75 % signal suppression for Cr(VI) and 25–50 % for Cr(III). In a pure aqueous solution the MIP Cr(VI) signal was by 20 % lower than that of Cr(III). These effects were lower using the ICP source, but they cannot be neglected. Easily ionizable matrix elements (Na, Ca) can cause 70 % signal suppression in the MIP, and 20 % in the ICP. Therefore, species dependent calibration is required in both cases. In the case of HPLC detection by MIP-OES, the detection limit was 13 ng for Cr(III), and 18 ng for Cr(VI). Using the ICP-OES detection, the detection limit was 0.2 ng for Cr (III) and 0.4 ng for Cr (VI). The linear dynamic ranges in both cases were two orders of magnitude.

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Shelar, Maruti, Vaibhav Gawade, and Santosh Bhujbal. "A Review on Heavy Metal Contamination in Herbals." Journal of Pharmaceutical Research International, May 15, 2021, 7–16. http://dx.doi.org/10.9734/jpri/2021/v33i29a31561.

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Nearly 70-80% of the world's population relies on non-conventional medicines, often derived from herbal plants, according to World Health Organization (WHO) figure. Medicinal plants are starting material for any herbal preparation such as herbal medicines, herbal teas, herbal oil etc. These preparations are being used worldwide due to their therapeutic potential and as they are considered to be safe as compared to allopathic medicines. Lead, cadmium, chromium, nickel, arsenic and mercury are the most common toxic metals that have become a matter of concern due to the reports of their contamination in various herbal preparations and herbal ingredients .Although the minimum acceptable limits have been laid out as safety concern. Heavy metal are evaluated by AAS, ICP-OES, ICP-MS, XRFS, HPLC, DPP, NAA, ASV and disposable sensor applications for achieving therapeutic efficacy and evolutions of heavy metal in herbals.

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Ivarsen, Elise, Christoffer P. Andersen, Sabine M. Jensen, Carsten T. Pedersen, and Anders K. Svaneborg. "Quantification of retinyl palmitate, thiamine, niacin, pyridoxine, folic acid, cyanocobalamin, zinc, and iron by chromatographic methods in fortified kernels and fortified rice." Acta Chromatographica, May 30, 2020. http://dx.doi.org/10.1556/1326.2020.00770.

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AbstractThis study presents the optimization and validation of methods for the analysis of retinol, thiamine, niacin, pyridoxine, folic acid, cyanocobalamin, zinc, and iron in fortified kernels (coated and extruded) and in fortified rice. The analyses were performed by HPLC-UV/FLD/MS and ICP-OES. The optimized methods showed good resolution of the analyte peaks, excellent recovery (87–108%), reproducibility with relative standard deviation (SD) of analyte content between 1.8 and 11% and high correlation coefficient of the calibration curves (R2 > 0.997). Limit of detection was from 2.8 E-4 mg/kg for pyridoxine to 1.26 mg/kg for zinc and limit of quantification was from 9.2 E-4 mg/kg for pyridoxine to 4.21 mg/kg for zinc. Thereby the optimized methods demonstrated reliability and sensitivity in the detection and quantification of these micronutrients and that they are suitable for routine analysis of fortified kernels (coated and extruded) and fortified rice.

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Fuentes Hernández, María Valentina. "Condición ambiental del sedimento del sector oriental del golfo de Cariaco." Revista Ciencias Marinas y Costeras, April 23, 2021, 57–76. http://dx.doi.org/10.15359/revmar.13-1.4.

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En 2008, se evaluó la condición ambiental del sedimento de la región oriental del golfo de Cariaco (Venezuela) al relacionar las concentraciones químicas de metales traza e hidrocarburos policíclicos aromáticos (HAP) con las respuestas a pruebas toxicológicas estandarizadas. Para ello, en abril, los metales particionados en el sedimento y en el agua retenida fueron extraídos y medidos con ICP-OES. Los HAP fueron separados del sedimento con ultrasonido y medidos con HPLC-fluorescencia. Además, se ejecutaron pruebas de supervivencia de Artemia y actividad hemolítica. Las concentraciones de metales totales (µg g-1) fueron: Cu (29-91), Cd (1.9-4.9), Cr (4-36 ), Ni (10-33), Pb (3-9) y Zn (32-112). En el agua fueron detectados, en µg L-1: Pb (33-56) y Cr (154-237). Las concentraciones de HAP (3-5 anillos) fueron bajas, con predominio de fluoranteno (22-71 ng g-1), en particular, pirolítico. Solamente, en algunas localidades, las concentraciones de Cu, Ni y Cd del sedimento superaron los criterios de calidad. No hubo mortandad de larvas durante las pruebas toxicológicas y la actividad hemolítica fue menor que 2%. Una vez realizado el contraste se demuestra que los sedimentos tienen alta calidad ecológica.

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Yang, Guiying, Farhan Ahmad, Qihuan Zhou, Meixia Guo, Shiyou Liang, Hassan Ahmed Gaal, and Jianchu Mo. "Investigation of Physicochemical Indices and Microbial Communities in Termite Fungus-Combs." Frontiers in Microbiology 11 (January 15, 2021). http://dx.doi.org/10.3389/fmicb.2020.581219.

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Termitomyces species are wild edible mushrooms that possess high nutritional value and a wide range of medicinal properties. However, the cultivation of these mushrooms is very difficult because of their symbiotic association with termites. In this study, we aimed to examine the differences in physicochemical indices and microbial communities between combs with Termitomyces basidiomes (CF) and combs without Termitomyces basidiomes (CNF). High-performance liquid chromatography (HPLC), inductively coupled plasma optical emission spectrometry (ICP-OES), gas chromatography equipped with a flame ionization detector (GC-FID), some commercial kits, high-throughput sequencing of the 16s RNA, and internal transcribed spacer (ITS) were used. Humidity, pH, and elements, i.e., Al, Ba, Fe, Mn, Ni, S, Ca, and Mg were higher while amino acids particularly alanine, tyrosine, and isoleucine were lower in CF as compared to CNF. The average contents of fatty acids were not significantly different between the two comb categories. The bacterial genera Alistipes, Burkholderia, Sediminibacterium, and Thermus were dominant in all combs. Brevibacterium, Brevundimonas, and Sediminibacterium were significantly more abundant in CF. Basidiomycota and Ascomycota were also identified in combs. Termitomyces clypeatus, Termitomyces sp. Group3, and Termitomyces sp. were the most dominant species in combs. However, any single Termitomyces species was abundantly present in an individual comb.

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Journal articles on the topic 'HPLC-ICP OES'

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