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Journal articles on the topic 'HPLC method'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Jinal, Harshadkumar Rathod *. Jaymin G. Patel Bhumi R. Patel. "ESTIMATION OF CLOTRIMAZOLE, CLINDAMYCIN PHOSPHATE AND TINIDAZOLE BY VARIOUS ANALYTICAL METHOD: REVIEW ARTICLE." Journal of Pharma Research 8, no. 4 (2019): 160–68. https://doi.org/10.5281/zenodo.2647870.

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<strong><em>ABSTRACT</em></strong> <strong><em>A</em></strong><em>nalytical method development and its validation is an important aspect in drug discovery process. Development of analytical method producing accurate and precise data is necessary to ensure the quality and safety of the drugs. At present, the most common analytical method employed for estimation of drugs is Reverse Phase High Pressure Liquid Chromatography (RP-HPLC) because of its high sensitivity, accuracy and speed. Different types of analytical methods are available for estimation of Clindamycin Phosphate, Clotrimazole and Ti

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2

Nikita, Pabale* Monali Khatke Mansi Shelke Vishweshwari Bhagat Tanvi Kamble. "A Review on HPLC Method Development and Validation." International Journal of Pharmaceutical Sciences, no. 11 (November 28, 2024): 1496–509. https://doi.org/10.5281/zenodo.14234238.

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High-Performance liquid chromatography (HPLC) is widely recognized as the most commonly used separation technique for the detection, separation, and quantification of drugs. To optimize HPLC methods, various chromatographic parameters are evaluated, including sample pretreatment, choice of mobile phase, column selection, and detector type. This article aims to discuss the processes involved in method development, optimization, and validation. The development and validation of analytical methods are critical in the discovery, development, and manufacturing of pharmaceutical drugs, as well as in

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3

Amol, Amrutkar, Aher Smita, and Bachhav Rishikesh. "Development And Validation Of UV-HPLC Method For The Estimation Of Diacerein In Bulk And Tablet Dosage Form." International Journal In Pharmaceutical Sciences 1, no. 1 (2022): 94–103. https://doi.org/10.5281/zenodo.6220056.

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<strong>Objective: </strong>Objective of the present analytical research work was to develop and validate Spectrophotometric method and High Performance Liquid Chromatographic method (HPLC Method) for the Diacerein bulk and tablet dosage form <strong>Methods: </strong>A spectrophotometric method and a HPLC method have been developed and validated for estimation of Diacerein in bulk. <strong>Method A (UV SPECTROMETRY Method): </strong>Methanol was used for the preparation of stock and working standard solutions of the drugs. 400-200nm UV range was used to scanned standard solutions of drugs usi

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4

Mahapatra, Asmita, and Chandanam Sreedhar. "HPLC AND ANALYTICAL METHOD VALIDATION : A REVIEW." Indian Research Journal of Pharmacy and Science 5, no. 1 (2018): 1399–414. http://dx.doi.org/10.21276/irjps.2018.5.1.18.

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K., Nagaraju, Mekala Sunil, Anusha M., Gopaiah Kurra Venkata, and Subbarao G. "Carvedilol, Tablets Assay Method Development and Validation by UV Spectroscopy, RP-HPLC and HPTLC." Trends in Pharmaceuticals and Nanotechnology (e-ISSN: 2582-4457) 2, no. 1 (2020): 37–47. https://doi.org/10.5281/zenodo.3632225.

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<em>Aim & Objectives : </em><em>The present study describes the development of a new rapid, simple, sensitive and reproducible RP-HPLC, HPTLC, UV Spectrophotometry method for the analysis of Carvedilol that offer certain advantages in its simplicity and sensitivity and applicable in routine analysis. Material & Methods: Carvedilol is polar in nature, reverse phase chromatography has been used and assay method is developed for UV-Spectroscopy, RP-HPLC, & HPTLC   & method validation is also done. Results & Discussion: The validated method was applied for the analysi

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Snyder, Lloyd R., Joseph L. Glajch, Joseph J. Kirkland, and Richard W. Abbott. "Practical HPLC method development." Analytica Chimica Acta 245 (1991): 287–88. http://dx.doi.org/10.1016/s0003-2670(00)80235-4.

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7

Krull, L. S. "Practical HPLC Method Development." TrAC Trends in Analytical Chemistry 8, no. 9 (1989): 349–50. http://dx.doi.org/10.1016/0165-9936(89)87046-3.

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8

Schoenmakers, Peter. "Practical HPLC method development." Journal of Chromatography A 509, no. 2 (1990): 406–7. http://dx.doi.org/10.1016/s0021-9673(01)93100-0.

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Kennedy, John F., and Maria da Paz C. Silva. "Practical HPLC method development." Carbohydrate Polymers 16, no. 3 (1991): 338. http://dx.doi.org/10.1016/0144-8617(91)90119-w.

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10

Akash, Shelake* Mrs. Jaya D. kamble Dr. Nilesh Chougule. "HPLC Development Method and Validation." International Journal of Pharmaceutical Sciences 2, no. 11 (2024): 910–19. https://doi.org/10.5281/zenodo.14203300.

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Chromatography, while primarily a separation technique, is widely used in chemical analysis. High-performance liquid chromatography (HPLC) is a highly versatile technology that separates analytes by passing them through a column filled with micrometer-sized particles. Today, reversed-phase chromatography is the most extensively utilized separation technique in HPLC. This is due to the reversed-phase method's simplicity, versatility, and scope, which can handle compounds with a wide range of polarity and molecular mass.This article discusses tactics and difficulties for planning HPLC method dev

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Sutandra, Salmon, Asvin Nurulita, and Mansyur Arif. "COMPARISON OF HBA1C LEVEL USING TURBIDIMETRY INHIBITION IMMUNOASSAY, LATEX AGGLUTINATION INHIBITION METHOD AND HPLC METHOD." INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY 24, no. 3 (2018): 269. http://dx.doi.org/10.24293/ijcpml.v24i3.1409.

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According to the National Glycohemoglobin Standardization Program (NGSP) and The Diabetes Control and Complication Trial(DCCT) the standard method of measuring HbA1c is High-Performance Liquid Chromatography (HPLC), but HPLC requiresparticular instrument investments, trained staff, long and relatively expensive sample processing. This an instrument that hassimilar performance to HPLC but its operation process is relatively simple and not costly. The purpose of this study was to analysethe HbA1c level using Turbidimetry Inhibition Immunoassay (TII) method and the HPLC method; to analyse HbA1 le

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Somshetwar, Kiran, and Mrinalini Damle. "Stability Indicating Eco-Friendly HPLC Method for Molnupiravir." International Journal of Science and Research (IJSR) 11, no. 3 (2022): 375–78. http://dx.doi.org/10.21275/sr22307172002.

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13

Rosano, T. G., R. T. Ambrose, A. H. Wu, T. A. Swift, and P. Yadegari. "Candidate reference method for determining creatinine in serum: method development and interlaboratory validation." Clinical Chemistry 36, no. 11 (1990): 1951–55. http://dx.doi.org/10.1093/clinchem/36.11.1951.

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Abstract We describe a "high-performance" liquid chromatographic (HPLC) method for accurately determining creatinine in serum. After prechromatographic precipitation of protein, we performed isocratic ion-exchange chromatography with ultraviolet detection (234 nm). Analytical results showed linearity up to 1770 mumol/L, a detection limit of 22 mumol/L, an average analytical recovery of 101%, and a CV ranging from 3% to 11%. We used certified human serum (National Institute of Standards and Technology), and additional lyophilized serum pools also assayed by definitive isotope-dilution mass spec

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Sagar, N. Katke*1 Santosh P. Kumbhar2 Vinod D. Usnale3 Siddhant M. Sawant4. "The Method Development And Validation Of A High-Performance Liquid Chromatographic Method For Azilsartan Analysis." International Journal in Pharmaceutical Sciences 2, no. 5 (2024): 1152–61. https://doi.org/10.5281/zenodo.11240815.

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The goal of the current study is to develop an RP-HPLC-based analytical method for determining the dosage of Azilsartan in bulk and tablet form that is fast, precise, sensitive, selective, and repeatable. Create a novel HPLC technique for Azilsartan estimation, and validate it in compliance with ICH guidelines. In order to make use of the accepted methodology for Azilsartan estimation in pharmaceutical formulations, an RP-HPLC method was utilized to create and validate a stability indicating method. Using an Inertsil-ODS C18 (250×4.6mm, 5µm) column and a 90:10 v/v methanol: acetoni

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sajid, raza. "Determination of Tizanidine in Transdermal Formulation by UV Spectrometry and High Performance Liquid Chromatography." Latin American journal of pharmacy 37, no. 11 (2018): 2154–8. https://doi.org/10.5281/zenodo.4659578.

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In current study UV and HPLC methods were developed for the quantitative estimation of tizanidine (TZD) in transdermal drug delivery patch. In order to improve the quantitative estimation, the sensitivity of the developed method was maximized by using suitable solvent system, specific wavelength of detection and precise chromatographic conditions. The regression equation obtained by UV method was found to be with absorbance = 2.88 × 102, concentration = 2.82 × 10–2 μg/mL while with HPLC method to be peak area = 3.6904 × 102, and concentration = 1.0342 × 103 ng/

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KIUCHI, Kazutoshi, and Toshiharu NAGATSU. "Progress in HPLC ECD method." Seibutsu Butsuri 30, no. 1 (1990): 40–43. http://dx.doi.org/10.2142/biophys.30.40.

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Livesey, J. F., J. G. Donnelly, and D. S. Ooi. "HPLC screening method for cystinuria." Clinical Chemistry 42, no. 10 (1996): 1714–16. http://dx.doi.org/10.1093/clinchem/42.10.1714.

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Strasters, J. K. "Computerized method development for HPLC." TrAC Trends in Analytical Chemistry 10, no. 3 (1991): x—xi. http://dx.doi.org/10.1016/0165-9936(91)85084-5.

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Amandeep, Kaur*and Monika Gupta. "DEVELOPMENT AND VALIDATION OF METHOD BY HPLC TECHNIQUES." INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES 05, no. 07 (2018): 7057–65. https://doi.org/10.5281/zenodo.1325897.

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<em>The primary focus of the review article is on general approaches and considerations toward development of chromatographic methods for separation, identification, and quantification of compounds, which may be applied within the various functions in the drug development continuum. This article also discusses the issues and parameters that must be considered in the validation of analytical methods. At the end of the review, a scope of the present research study is covered.</em> <strong>Keywords: </strong><em>Introduction, Method development, Steps for HPLC method development, Method validatio

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Vinayak, A. katekar, P. Kothari Prafful, S. Kawarkhe Swapnil, P. Surung Manish, and B. Akotkar Vaishnavi. "A review on method development and validation of stability indicating RP HPLC Method for metformin and Empagliflozin." GSC Biological and Pharmaceutical Sciences 24, no. 1 (2023): 310–18. https://doi.org/10.5281/zenodo.8265469.

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Metformin HCl and empagliflozin are oral antidiabetic medications that help control blood sugar levels. A simple, precise, rapid, accurate, sensitive, specific, and stable RP-HPLC method was developed and validated for the simultaneous determination of Metformin and Empagliflozin in drug bulk form and drug dosage. This new RP-HPLC method is superior to formal reversed-phase HPLC with reduced solvent usage, retention time, resolution, and cost. Upper region separation was performed on a Waters HPLC system equipped with a PDA detector and autosampler. Both Metformin and Empagliflozin undergo str

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Vinayak A. katekar, Prafful P. Kothari, Swapnil S. Kawarkhe, Manish P. Surung, and Vaishnavi B. Akotkar. "A review on method development and validation of stability indicating RP HPLC Method for metformin and Empagliflozin." GSC Biological and Pharmaceutical Sciences 24, no. 1 (2023): 310–18. http://dx.doi.org/10.30574/gscbps.2023.24.1.0233.

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Metformin HCl and empagliflozin are oral antidiabetic medications that help control blood sugar levels. A simple, precise, rapid, accurate, sensitive, specific, and stable RP-HPLC method was developed and validated for the simultaneous determination of Metformin and Empagliflozin in drug bulk form and drug dosage. This new RP-HPLC method is superior to formal reversed-phase HPLC with reduced solvent usage, retention time, resolution, and cost. Upper region separation was performed on a Waters HPLC system equipped with a PDA detector and autosampler. Both Metformin and Empagliflozin undergo str

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Kim, Yoo-Gon, Houng-Seok Seo, and Chan-Hee Won. "Analytical method validation of oxiracetam using HPLC." Analytical Science and Technology 23, no. 6 (2010): 587–94. http://dx.doi.org/10.5806/ast.2010.23.6.587.

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KHANDARE, BALU, Atish C. Musle, Sanket S. Arole, and Pravin V. Popalghat. "Analytical method development and validation of olmutinib bulk drug as per ICH Q2 guidelines by using RP-HPLC Method." Journal of Drug Delivery and Therapeutics 9, no. 4-A (2019): 608–11. http://dx.doi.org/10.22270/jddt.v9i4-a.3527.

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RP-HPLC is fast, simple, sensitive, precise, and reproducible (liquid chromatography) method, developed and validated to analyseolmutinib bulk dosage form. Using C-18 HPLC column separation was carried out. This was maintained at ambient temperature. During separation mobile phase consist of methanol (100 v/v) was delivered at a rate of 1mL/min. Using UV detector analysis was carried out at the wavelength 267.68 nm. RP-HPLC method was validated by using various parameters like, precision, limit of quantitation (LOQ), linearity and robustness. The RP-HPLC method was found to be linear over the

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Dračková, M., I. Borkovcová, B. Janštová, et al. "Determination of Lactoferrin in Goat Milk by HPLC Method." Czech Journal of Food Sciences 27, Special Issue 1 (2009): S102—S104. http://dx.doi.org/10.17221/944-cjfs.

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The aim of this study was the determination of lactoferrin in goat milk using HPLC method. Milk samples were collected at a goat farm in the South Moravia Region, the Czech Republic. It were established bulk tank samples of raw milk (<I>n</I> = 24) and pasteurised milk (<I>n</I>n = 27) that were collected during lactation. Lactoferrin contents were analysed by reverse phase high-performance liquid chromatography (RP-HPLC) with diode-array detector PDA 2996. Detection was carried out at the wavelength 205 nm. The average concentration of lactoferrin in goat milk was 120

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Parmar, Raghuveersinh, Dr Priyanka Patil, Dr Chainesh Shah, and Dr Umesh Upadhyay. "A Review Article on Method Development and Validation of Verapamil by RP-HPLC Method." International Journal of Pharmaceutical Research and Applications 09, no. 05 (2024): 977–88. https://doi.org/10.35629/4494-0905977988.

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High performance liquid chromatography, one of the most powerful analytical techniques utilized in the separation, identification, and quantification of complex mixtures. Reverse-phase or RP-HPLC is commonly practiced in high performance liquid chromatography because this technique has the advantages of versatility and appropriateness for more hydrophobic compounds. Fundamentals and Practices of Reversed-Phase HPLC: Part II -- RPHPLC column configurations. It consists of the concept of separation, types of stationary and mobile phases with the variables concerned with the separation. This revi

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*Krishnaphanisri, Ponnekanti, Anusha Addanki, Raj Kamal B., Bhavani Dasari Durga, Reddy Gargula Sumana, and Reddy Karnati Shiva. "A REVIEW-ON METHOD DEVELOPMENT & METHOD VALIDATION BY RP-HPLC FOR ESTIMATION OF ARFORMOTEROL." World Journal of Pharmaceutical Science and Research 4, no. 2 (2025): 1062–70. https://doi.org/10.5281/zenodo.15364947.

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Arformoterol is a long-acting β<sub>2</sub> adrenoreceptor agonist (LABA) and it is the active (<em>R</em>,<em> R</em>)-(−)enantiomer of formoterol. It was approved for medical use in the United States in October 2006. It is available as a generic medication. Arformoterol is indicated for the maintenance treatment of bronchoconstriction in people with chronic obstructive pulmonary disease (COPD). The purpose of analytical method development is to establish the identity, purity, physical characteristics, and potency of drugs, including the drug’s bioavailability and stability.

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Agarwal, Rajiv. "Rapid microplate method for PAH estimation." American Journal of Physiology-Renal Physiology 283, no. 2 (2002): F236—F241. http://dx.doi.org/10.1152/ajprenal.00336.2001.

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Evaluation of renal hemodynamics requires estimation of effective renal plasma flow, which is commonly measured by the renal clearance of p-aminohippuric acid (PAH). There are many existing methods for PAH assay that are complicated, expensive, or time consuming. We describe a rapid, precise, and accurate microplate-based assay of PAH using p-dimethylaminocinnamaldehyde, which produces a red color on reaction with PAH, and compare it with a reference HPLC method. Renal PAH clearances were measured in 10 volunteers, and clearances were calculated by using the new and HPLC methods. There was exc

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Swami, Pramod, Dilip Agrawal, Rakesh Goyal, Gaurav Bhaduka, and Mohit Khandelwal. "Method development and validation of RP-HPLC method in tablet dosage form: A Review." Pharmaceutical and Chemical Journal 9, no. 5 (2022): 48–57. https://doi.org/10.5281/zenodo.13973791.

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HPLC is the dominant separation technique for drug detection, separation and quantification. To optimize the method, a number of chromatographic parameters were analyzed, such as sample pre-treatment, selection of mobile phase, columns, detector selection. The aim of this article is to review the development, optimization and validation of the method. HPLC method development depends on the chemical structure of molecules, synthetic route, solubility, polarity, pH and pKa values and activity of functional groups, etc. Validation of HPLC method according to ICH guidelines provides information on

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Gunjan, Rao, and Goyal Anju. "An Overview on Analytical Method Development and Validation by Using HPLC." Pharmaceutical and Chemical Journal 3, no. 2 (2016): 280–89. https://doi.org/10.5281/zenodo.13754273.

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HPLC is an analytical technique widely used for identification, separation, detection and quantification of various drugs and its related degradants. HPLC process development is important in case of drug discovery, drug development and in analysis of pharmaceutical products. It is also employed to separate the manufactured drugs and its related impurities. It also involves the understanding of physicochemical properties of drug substances; hence facilitate the development of analytical method. The article mainly focuses on optimization of HPLC method during process development and validation o

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Ningrum, Vitarani Dwi Ananda, Senya Puteri Amalia, and Ari Wibowo. "Vancomycin bioanalysis for TDM services by using immunoassay and HPLC: A scoping review." Pharmacy Education 24, no. 3 (2024): 197–203. http://dx.doi.org/10.46542/pe.2024.243.197203.

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Background: Administration of vancomycin in treating infections caused by Methicillin-resistant Staphylococcus aureus (MRSA) requires therapeutic drug monitoring (TDM). The immunoassay method and high-performance liquid chromatography (HPLC) are the two methods of choice for examining vancomycin levels, with their respective advantages. Objective: This study aims to review the validity of immunoassay and HPLC methods, as well as consider which method is appropriate, effective, and efficient for TDM in the clinical setting. Method: Related articles were searched for using the keywords "immunoas

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Mayuri, Bhokare* Dr. Pradyumna Ige. "Analytical Method Development and Validation of Dapagliflozin By RP-HPLC Method In Tablet Dosage Form." International Journal of Pharmaceutical Sciences 2, no. 7 (2024): 1718–28. https://doi.org/10.5281/zenodo.12801306.

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The main aim of the present research work is to develop a sensitive, precise and accurate HPLC (High-Performance Liquid Chromatography) procedure for the selective estimation of Dapagliflozin. An isocratic separation of Dapagliflozin through column used was a Inertsil ODS-3V with dimensions of 150 mm length and 4.6 mm inner diameter, packed with 5μm particle utilizing mobile phase composition of Acetonitrile and Water, with a proportion of 50% Acetonitrile and 50% water (v/v).  The detection of the analyte was processed at the maximum wavelength of 223 nm and with 1 ml/min flow of the

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D., Kawade*1 Aishwarya Shrirao2 Irshad Ahmad Mohd Salim3. "Comprehensive Review on Analytical Method Development and Validation: Focus on HPLC Techniques." International Journal of Pharmaceutical Sciences 3, no. 2 (2025): 1444–58. https://doi.org/10.5281/zenodo.14883977.

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High Performance thin-layer liquid chromatography refers to a feasible, sophisticated analytical method established upon thin-layer chromatography (TLC) concepts that offers several important advantages, including better material application, quicker and more efficient separation and lower mobile phase usage. The intent of this investigation had been to provide a quick as well as easy HPTLC approach to Piroxicam identification. The suggested technique can be applied to detect the substances under analysis at extremely low quantities. The suggested approach is straightforward, eco-friendly, sta

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Mohd., Ibrahim, Goyal Rakesh, and Agarwal Dilip. "DEVELOPMENT AND VALIDATION BY HPLC METHOD." International Journal of Current Pharmaceutical Review and Research 14, no. 03 (2022): 104–7. https://doi.org/10.5281/zenodo.12658787.

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Validation of an analytical procedure is the process of demonstrate with suitable for itsintended purpose. Chromatographic such HPLC methods play significant role in thepharmaceutical industry from the drug discovery, development of drugs, formulations andquality control of chemicals. Many validated analytical methods ensure that it providesconsistent, reliable and accurate data for results. So these methods help pharmaceuticalanalyst to ensure quality products are released for market. This review explains generalapproach for validation process and validation parameters to be considered during

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Antunes, Marina Venzon, Sandrine Comparsi Wagner, Joíza Lins Camargo, and Rafael Linden. "Standardization of method for determining glycosylated hemoglobin (Hb A1c) by cation exchange high performance liquid chromatography." Brazilian Journal of Pharmaceutical Sciences 45, no. 4 (2009): 650–57. http://dx.doi.org/10.1590/s1984-82502009000400007.

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Hemoblobin A1c is the most important parameter for the monitoring of metabolic control of patients with diabetes mellitus. The purpose of this study was to adapt the Mono S method to a conventional HPLC system, allowing highly selective HbA1c determination without the acquisition of kits or the use of dedicated systems The results obtained were compared to the Tinaquant® immune turbidimetric method and the Bio-Rad Variant® chromatographic method. The developed method presented intra-study precision (C.V. %) of 1.39-3.65 and inter-study precision (C.V. %) of 2.80-3.02%. The determination coeffi

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Madhuri, R. Shirsath Sonali A. Waghmare And Pradyumna P. Ige. "A Review On Recent Advances In Development Of RP-HPLC Method." International Journal of Pharmaceutical Sciences 2, no. 8 (2024): 2674–82. https://doi.org/10.5281/zenodo.13242715.

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The development and validation of analytical methods play crucial roles in drug discovery, drug development, and the production of different pharmaceutical formulations. A straightforward, accurate, exact, and targeted RP-HPLC approach was created to determine the drug in pharmaceuticals. Because of its greater selectivity and flexibility for hydrophobic substances, reverse-phase liquid chromatography (RP-HPLC) is the separation method of choice for high-performance liquid chromatography (HPLC). The development of RP HPLC methods has advanced significantly in recent years, with new stationary

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Macko, T., and D. Berek. "New Diagnostic Method for HPLC Colum." Journal of Chromatographic Science 25, no. 1 (1987): 17–20. http://dx.doi.org/10.1093/chromsci/25.1.17.

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Thimm, N., J. Handl, and M. Binder. "HPLC-MS-Method for A-Trichothecenes." Mycotoxin Research 17, S2 (2001): 206–9. http://dx.doi.org/10.1007/bf03036437.

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Rajadhyaksha, Namita S., Satish P. Jain, and Purnima D. Amin. "Carbamazepine: Stability Indicating HPLC Assay Method." Analytical Letters 40, no. 13 (2007): 2506–14. http://dx.doi.org/10.1080/00032710701583557.

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Mutton, I. M. "“Practical HPLC method development”, 2nd edition." Chromatographia 47, no. 3-4 (1998): 234. http://dx.doi.org/10.1007/bf02466588.

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SREEJANARDHANAN.V and SUDEV SHINE. "RP UFLC method for estimation of valsartan chemometricaly." World Journal of Advanced Research and Reviews 18, no. 2 (2023): 415–26. https://doi.org/10.5281/zenodo.8404758.

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A simple, sensitive, rapid, precise and economical reverse phase HPLC method has been developed for the estimation of valsartan from pharmaceutical dosage forms. The method was carried out  using Chromatographic conditions were established by employing a C18 analytical phenomenal (Kinetex) column (5micron -C18, 250x4.6mm) with a mobile phase of phosphate buffer (PH 3.5) and acetonitrile in a 60:40 ratio. The sample was injected at a volume of 20 microliters, and the mobile phase was degassed using a Sonica ultrasonic sonicator before being pumped into the HPLC system. The flow rate was se

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Qin, Yi, Hong-ling Zhang, Jin Wang, Qin-wan Huang, Ji-lin Sun, and Ren-chuan Yao. "Chromatographic determination of siphonodin content: A rapid and simple strategy for discriminating between Hemsleya omeiensis and other sources of Xuedan." Tropical Journal of Pharmaceutical Research 18, no. 4 (2021): 837–42. http://dx.doi.org/10.4314/tjpr.v18i4.22.

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Purpose: To develop a rapid and simple siphonodin content-based high performance liquid chromatography (HPLC) method to distinguish Hemsleya omeiensis from other sources of xuedan. Methods: Siphonodin was isolated from Hemsleya omeiensis and identified by x-ray crystallographic analysis. An optimized HPLC method was applied for the determination of siphonodin contents of H. omeiensis, H. dolichocarpa and H. gigantha. Results: Siphonodin was successfully separated by the optimized HPLC method in < 10 min, and the results of validation showed that the HPLC method was stable and ve

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Kumar, Dr Meruva Sathish, Ch Ajay Kumar, Naik Parth Pravin, N. Swarnalatha, and Afsheen Nida. "Simultaneous Estimation of Acetaminophin and Benzhydrocodone by RP-HPLC Method." International Journal of Research Publication and Reviews 5, no. 6 (2024): 6053——6062. http://dx.doi.org/10.55248/gengpi.5.0624.1620.

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Ramya, Sudha P., P. Bharath, and D. Ramachandran. "A validated RP-HPLC method for quantitative determination of genotoxic impurity hydrazine content in Ursodeoxycholic acid." Research Journal of Chemistry and Environment 29, no. 5 (2025): 160–68. https://doi.org/10.25303/295rjce1600168.

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A highly sensitive method for the determination of genotoxic impurity such as hydrazine in Ursodeoxycholic acid using RP-HPLC has been presented in the present study. Quantification of hydrazine content in Ursodeoxycholic acid sample by HPLC was done with UV Detector. Hydrazine was UV inactive compound. Derivatization procedure was established to detect the hydrazine in HPLC. For this, 4-Hydroxy 3-methoxybenzaldehyde was used as a derivatizing agent which reacts with hydrazine in the presence of disodium tetraborate to form a compound which was UV active. Hydrazine was determined by RP-HPLLC m

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Оlena, Mamina, and Kabachny Volodimir. "Identification and quantitative determination of clonidine by HPLC method." ScienceRise: Pharmaceutical Science, no. 5(27) (October 30, 2020): 30–36. https://doi.org/10.15587/2519-4852.2020.215101.

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<strong>The aim</strong> of this work is identification and quantification of clonidine hydrochloride by a unified HPLC method, which allows to obtain reliable and reproducible research results. <strong>Materials and methods. </strong>HPLC analysis was carried out on a microcolumn liquid chromatograph "Milichrome A-02" in conditions: reversed-phase variant, column with non-polar sorbent Prontosil 120-5 C<sub>18</sub> AQ, 5 μm; mobile phase in the mode of linear gradient – from eluent А (5 % acetonitrile and 95 % buffer solution) to eluent B (100 % acetonitrile

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Bollepaka, Srilatha Santhosh Illendula. "STABILITY INDICATING METHOD DEVELOPMENT OF RIMONABANT AND IT's VALIDATION BY RP-HPLC AND HPTLC METHOD." INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES 05, no. 06 (2018): 5602–10. https://doi.org/10.5281/zenodo.1297120.

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A new simple, accurate, rapid, precise, reproducible and cost effective HPLC and HPTLC method for the quantitative estimation of Rimonabant in bulk and pharmaceutical dosage form. In Method using HPTLC, chromatograms were developed in a mobile phase of 2 mM Sodium dihydrogen orthophosphate (NaH2PO4)-Acetonitrile (38:62) for approximately 25 mins and detected at λmax of 303 nm. Accuracy was found to be in the range of 98.31-102.25 % whereas intraday precision was found to be in the range of 0.03 to 1.60%. Interday precision was found to be 1.36, 0.74 and 0.31 at LQC, MQC and HQC, respect

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Manjrawala, Mitali, Srinivasarao V. N. Divvela, and Rakesh Kumar. "Validated RP-HPLC Stability Indicating Method of Anti-Diabetic Active Pharmaceutical Ingredient; Saxagliptin Hydrochloride Dihydrate." DER PHARMA CHEMICA 13, no. 3 (2021): 5. https://doi.org/10.5281/zenodo.11071036.

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A simple and precise stability-indicating reverse phase high performance liquid chromatography (RP-HPLC) method was developed and validated for the determination of related substances of Saxagliptin drug substance. The method was developed to separate possible degradation and process related impurities. Chromatographic condition was carried out on in Zorbax SB-C8, 150 mm x 4.6 mm, 5µm HPLC column with prepared buffer solution 1.0 ml of ortho phosphoric acid mixed with 0.1% of sodium dihydrogen phosphate solution, mix buffer solution and acetonitrile in ratio of 70:30(%v/v) used as mobile

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Manjrawala, Mitali, N. Divvela Srinivasarao V, and Rakesh Kumar. "Validated RP-HPLC Stability Indicating Method of Anti-Diabetic Active Pharmaceutical Ingredient; Saxagliptin Hydrochloride Dihydrate." Der Pharma Chemica 13, no. 3 (2021): 5. https://doi.org/10.5281/zenodo.14722741.

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A simple and precise stability-indicating reverse phase high performance liquid chromatography (RP-HPLC) method was developed and validated for the determination of related substances of Saxagliptin drug substance. The method was developed to separate possible degradation and process related impurities. Chromatographic condition was carried out on in Zorbax SB-C8, 150 mm x 4.6 mm, 5µm HPLC column with prepared buffer solution 1.0 ml of ortho phosphoric acid mixed with 0.1% of sodium dihydrogen phosphate solution, mix buffer solution and acetonitrile in ratio of 70:30(%v/v) used as mobile

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Kotal, F., and Z. Radová. "A simple method for determination of deoxynivalenol in cereals and flours." Czech Journal of Food Sciences 20, No. 2 (2011): 63–68. http://dx.doi.org/10.17221/3511-cjfs.

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An effective and fast method for determination of deoxynivalenol (DON) in cereals and flours has been developed. The immunoaffinity column was used for the isolation of DON from wheat, corn, rice and flour extract. The determination was carried out by using the HPLC/UV method. The limit of detection was 0.02 mg/kg. The recoveries for the assay range 0.1 to 2 mg/kg were generally higher than 80%, ranging from 83 to 96% with an average relative standard deviation of 3.8%. The trueness of the method using the DON test &ndash; HPLC column was established by use of certified reference material

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Badhe, Pallavi, Smita Aher, and Ravindranath B. Saudagar. "Development and Validation of Spectrophotometric and Chromatographic Method for the Estimation of Apremilast in Bulk and Formulations." Journal of Drug Delivery and Therapeutics 9, no. 6-s (2019): 136–42. http://dx.doi.org/10.22270/jddt.v9i6-s.3777.

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Objective: Objective of the present analytical research work was to develop and validate Spectrophotometric method and High Performance Liquid Chromatographic method (HPLC Method) for the Apremilast bulk and tablets dosage form. Methods: A spectrophotometric method and a HPLC method have been developed and validated for estimation of APR in pharmaceutical oral dosage form. Method A (UV SPECTROMETRY Method): The stock and working standard solutions of the drugs were prepared in methanol. Standard solutions were scanned over the range of 400-200 nm in spectrum mode of spectrophotomet

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Aditi, Gade* Prasanna Datar. "A Review on Analytical Method Development for Drug Sitagliptin." International Journal of Pharmaceutical Sciences 3, no. 4 (2025): 2713–21. https://doi.org/10.5281/zenodo.15263891.

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The Sitagliptin offer significant therapeutic benefits in managing type 2 diabetes mellitus (T2DM) and associated cardiovascular risks. The development of accurate, precise, and validated analytical methods is crucial for ensuring their efficacy, safety, and regulatory compliance. This review explores various analytical techniques, including High-Performance Liquid Chromatography (HPLC), Reverse Phase HPLC (RP- HPLC), High-Performance Thin Layer Chromatography (HPTLC), Liquid Chromatography-Mass Spectrometry (LC-MS/MS), and UV spectrophotometry, highlighting their advantages, challenges, and v

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