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Journal articles on the topic 'HPLC-RID'

Author: Grafiati

Published: 5 June 2025

Last updated: 15 July 2025

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1

Umur, H., H. Hanoğlu Oral, H. Ekşi Karaağaç, S. Koçer, HÖ Uçurum, and E. Altınçekiç. "Determination of Urea Content in Dairy Cattle Feed by High Performance Liquid Chromatography via Refractive Index Detector: Method Optimization, Validation and Comparison with Spectro-Colorimetric Method." Journal of the Hellenic Veterinary Medical Society 75, no. 2 (2024): 7501–12. http://dx.doi.org/10.12681/jhvms.35131.

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The aim of this study was to analyze the urea content of Dairy Cattle Feed (DCF) using High Performance Liquid Chromatography via Refractive Index Detector (HPLC-RID), without derivatization, along with spectro-colorimetric method that is the official control method of European Union (EU). Towards that goal, the analysis procedure for the method was established, method optimization and validation were carried out by spiking urea at 2% level to DCF, which is the upper limit allowed for use in ruminant feeds in Türkiye. In method validation; R2: 0.9997 coefficient of determination at 25-100000 m

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2

Armoogum, V., and K. Boodhoo. "Full optimization and validation of an HPLC method for the quantitative analysis of total sugars in a soft drink." Bulletin of the Chemical Society of Ethiopia 34, no. 2 (2020): 419–26. http://dx.doi.org/10.4314/bcse.v34i2.17.

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Five HPLC methods were employed for the quantitative analysis of three natural sugars namely fructose, glucose and sucrose in soft drinks. HPLC-refractive index detector (RID)-AMINO proved to be the most suitable HPLC method to carry out the latter task. For the optimum separation and response of the natural sugars the best conditions employed were column oven temperature 30 oC, flow rate 0.1 mL/min, mobile phase ratio acetonitrile:water 75:25 and they were determined by studying all possible interactions among these three parameters. Full validation of HPLC-RID-AMINO was performed in terms of

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3

Narasimha, Swamy Lakka, N. V. V. D. Praveen B., and Reddy Chinta Srinivasa. "Simple and sensitive RP-HPLC/RID method for the determination of triclofos sodium." Journal of Indian Chemical Society Vol. 94, Jan 2017 (2017): 53–58. https://doi.org/10.5281/zenodo.5603385.

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Analytical Research and Development, Quality Control, Quality Management, PSAI-Quality, Dr. Reddy’s Lab. Ltd., Hyderabad-500 072, India <em>E-mail</em> : nslakka@gmail.com <em>Manuscript received online 13 August 2016, accepted 30 September 2016</em>  A simple and sensitive RP-HPLC method was developed and validated for the determination of triclofos sodium in oral solution using ‘Watman’ Partisil SCX (250×4.6 mm, 5µm) with a mobile phase of 0.01% formic acid buffer with pH 3.5 with a flow rate of 0.5 mL/min (RI detection). The proposed method provided linear

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4

Martins, Pablo H. S., Maria A. Barros, Caroline L. Silva, et al. "A cellulose monolithic stir bar for sorptive extraction of glycerol from biodiesel." RSC Advances 14, no. 25 (2024): 17380–88. http://dx.doi.org/10.1039/d4ra02985b.

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Cellulose monoliths were used as stir-bar adsorbents in SBSE to extract glycerol from biodiesel samples with HPLC-RID analyses. The proposed method dispenses derivatizing reagents or sophisticated apparatus and uses water as the desorption eluent.

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5

Bilskey, Sarah R., Samantha A. Olendorff, Karolina Chmielewska, and Kevin R. Tucker. "A Comparative Analysis of Methods for Quantitation of Sugars during the Corn-to-Ethanol Fermentation Process." SLAS TECHNOLOGY: Translating Life Sciences Innovation 25, no. 5 (2020): 494–504. http://dx.doi.org/10.1177/2472630320908253.

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The quantitation of sugars, including glucose, the primary fermentable sugar; maltose (DP2); and maltotriose (DP3), is a standard procedure during the corn-to-ethanol fermentation process. The quantitation of glucose by the Megazyme Assay utilizing glucose oxidase and peroxidase enzymes (GOPOD) and UV-Vis detection, high-performance liquid chromatography with refractive index detection (HPLC-RID), and liquid chromatography mass spectrometry (LC-MS) with electrospray ionization (ESI) and selected ion monitoring (SIM) was studied. Three biological flask fermentation replicates were analyzed ever

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6

Silveira, Michelle Fernandes, Lourdes Maria Pessôa Masson, José Francisco Pereira Martins, et al. "Simultaneous Determination of Lactulose and Lactose in Conserved Milk by HPLC-RID." Journal of Chemistry 2015 (2015): 1–6. http://dx.doi.org/10.1155/2015/185967.

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Heat treatment is applied to dairy products to ensure microbiological quality and increase the shelf life. However, a suitable control of this process is necessary to guarantee nutritional and sensory quality. The aim of this study is to adapt the high performance liquid chromatography (HPLC) method for determination of lactulose and lactose content in commercial samples of UHT and sweetened condensed milk. The HPLC method used showed a good resolution of the analytes evaluated. The analyzed UHT milk samples presented levels for lactulose in accordance with the limit recommended by the Interna

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7

Cheng, Qian, Shuhuan Peng, Fangyi Li, et al. "Quality Distinguish of Red Ginseng from Different Origins by HPLC–ELSD/PDA Combined with HPSEC–MALLS–RID, Focus on the Sugar-Markers." Separations 8, no. 11 (2021): 198. http://dx.doi.org/10.3390/separations8110198.

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Red ginseng (RG) has been extensively utilized in Asian countries due to its pharmacological effects. For the quality evaluation of RG, small molecules, such as ginsenosides, have been widely considered as candidates of its quality markers (Q-markers), and various analytical techniques have been developed in order to identify these compounds. However, despite the efforts to analyze the hydrophobic constituents, it is worth pointing out that about 60% of the mass of RG is made of carbohydrates, including mono-, oligo- and polysaccharides. Consequently, the quality differentiation and identifica

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8

Petkova, Nadezhda. "CHARACTERIZATION OF INULIN FROM BLACK SALSIFY (SCORZONERA HISPANICA L.) FOR FOOD AND PHARMACEUTICAL PURPOSES." Asian Journal of Pharmaceutical and Clinical Research 11, no. 12 (2018): 221. http://dx.doi.org/10.22159/ajpcr.2018.v11i12.28262.

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Objective: The aim of the current study was to evaluate the black salsify (Scorzonera hispanica L.) as a potential source of inulin and to characterize the physicochemical properties of isolated polysaccharide.Methods: The carbohydrate content in its roots and leaves was analyzed by high-performance liquid chromatography with refractive index detection (HPLC-RID) method. Microwave-assisted extraction was performed for isolation of inulin from black salsify roots. The obtained polysaccharide was characterized by HPLC-RID method, HPLC size-exclusion chromatography, and Fourier transformed-infrar

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9

Xie, Yuesheng, Dayong Zheng, Ting Yang, et al. "Head-to-Head Comparison of High-Performance Liquid Chromatography versus Nuclear Magnetic Resonance for the Quantitative Analysis of Carbohydrates in Yiqi Fumai Lyophilized Injection." Molecules 28, no. 2 (2023): 765. http://dx.doi.org/10.3390/molecules28020765.

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Carbohydrate analysis can be used as a standard analysis for quality control of industries of plants, foods and pharmaceuticals. Quantitative 1H NMR spectroscopy (qNMR) is an excellent alternative to chromatography-based mixture analysis. However, the application of qNMR in sugar analysis has rarely been reported. In this study, the performance of qNMR in sugar analysis was investigated and compared with the results from HPLC analysis. A head-to-head comparison of qNMR (internal and external standard methods) versus HPLC (PMP pre-column derivatization HPLC, HPLC-RID and HPLC-ELSD) based on qua

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10

Imeri, Shkendije, Snezana Zaharieva, Gordana Majnova, et al. "Overview of the compendial methods for quantitative determination of glucose in solutions for intravenous infusion." 68 68, no. 02 (2022): 47–51. http://dx.doi.org/10.33320/maced.pharm.bull.2022.68.02.005.

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This paper provides a review of the pharmacopoeial methods for determination of the content of glucose in parenteral solutions (HPLC/RID method, polarimetry and volumetric titration) and evaluation of all critical aspects of the proposed methods, with a focus on accuracy, precision, time of analysis and resources for introducing, maintenance and conducting analysis. Comparative analysis of the results from assay (ten independent determinations) of Glucose 5% w/v solution for intravenous infusion, obtained using all three methods, was performed. The analysis confirmed that there is no statistic

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Hidayat, Basultan, Minda Azhar, Iryani Iryani, and Hary Sanjaya. "Penentuan Derajat Polimerisasi Inulin dari Umbi Dahlia (dahlia sp.) Menggunakan Metode HPLC Dengan Detektor RID." Jurnal Periodic Jurusan Kimia UNP 10, no. 1 (2021): 54. http://dx.doi.org/10.24036/p.v10i1.109604.

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The degree of polymerization (DP) of inulin plays important rolein inulin utilization.This study aims to determine and compare DP of inulin from dahlia tubers that wereextracted in fresh and extracted after storaging in 15 days.Extraction of inulin is carried out based on its solubility in hot water.DP of inulin was measured using a High Performance Liquid Chromatography (HPLC) instrument with variety of measurement conditions. HPLC with RID detector, Hi-plex H Column, 100% aquabidest mobile phase, flow rate of 0.6 ml / minute, and injection volume of 20 µl gave the best measurement results. D

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Paryanti, Sri, Benny Permana, and Sophi Damayanti. "Development and validation of analytical procedure for analysis of polyvinylpyrrolidone in tablets containing metformin hydrochloride and pioglitazone hydrochloride." Pharmacia 72 (May 22, 2025): 1–10. https://doi.org/10.3897/pharmacia.72.e122950.

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Polyvinylpyrrolidone (PVP) is a common excipient in pharmaceutical formulations due to its inert, biocompatible, and multifunctional properties. However, its presence can impact drug release. This study aims to optimize the analysis method for determining PVP concentration in tablets containing a combination of metformin hydrochloride and pioglitazone hydrochloride. The research involved optimizing stationary and mobile phases using HPLC, detector optimization, sample preparation, and stability testing. Validation followed ICH guidelines, covering system suitability, specificity, linearity, ac

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13

Bokov DO, Bokov, Sergunova Sergunova EV, Marakhova Marakhova AI, et al. "Determination of Sugar Profile in Viburnum Fruits and its Dosage Forms by HPLC-RID." Pharmacognosy Journal 12, no. 1 (2020): 103–8. http://dx.doi.org/10.5530/pj.2020.12.16.

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14

LI, Wei-Tao, Jian-Bin GUO, Bo-Lun YU, et al. "Establishment of HPLC-RID method for the determination of soluble sugars in peanut seed." Acta Agronomica Sinica 47, no. 2 (2020): 368–75. http://dx.doi.org/10.3724/sp.j.1006.2021.04110.

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15

Perova, I. B., K. I. Eller, M. A. Gerasimov, et al. "A study of a complex of bioactive compounds in the fruits of promising blue honeysuckle (<i>Lonicera caerulea</i>l.) cultivars." Proceedings on applied botany, genetics and breeding 184, no. 1 (2023): 53–69. http://dx.doi.org/10.30901/2227-8834-2023-1-53-69.

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Background. Blue honeysuckle (Lonicera caerulea L.) is a valuable source of bioactive compounds (BAC) of polyphenolic nature and rare for horticultural berries iridoids, which have antioxidant, anti-inflammatory, antimicrobial and other properties and are widely used in the food, medical and cosmetic industries.Materials and methods. The berries of 20 released honeysuckle cultivars of Russian, Canadian and U.S. origin, reproduced at the I.V. Michurin Federal Science Center in Tambov Province, were studied for the content of the main BAC groups using modern methods (spectrophotometry, HPLC-UV,

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16

Piszcz, Paweł, Konrad Żurawski, and Bronisław K. Głód. "Application of HPLC to Study the Reaction of Free Radicals with Antioxidants and/or Toxins." Journal of Chemistry 2014 (2014): 1–6. http://dx.doi.org/10.1155/2014/385908.

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The application of HPLC in the antioxidative (antiradical) properties studies of various samples is discussed in this paper. The assay is based on the reaction of, characterized by strong oxidizing properties, hydroxyl radicals (generated in the Fenton-like reaction) with the sample and with the, so-called, sensor. The product of the sensor reaction with the radicals is analyzed using RP-HPLC with fluorescence detection. It is well known that antioxidants, which are healthy for living organisms, have a negative environmental effect. Therefore, the goal of the paper is to discuss if the same se

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17

Felföldi, Zoltán, Floricuta Ranga, Sonia Ancuta Socaci, et al. "Physico-Chemical, Nutritional, and Sensory Evaluation of Two New Commercial Tomato Hybrids and Their Parental Lines." Plants 10, no. 11 (2021): 2480. http://dx.doi.org/10.3390/plants10112480.

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Tomato (Solanum lycopersicum) is the globally most consumed vegetable. The objective of this research was to analyze physico-chemical, nutritional and sensorial components (taste and flavor) in two new commercial hybrids (AS 300 F1 and AS 400 F1) and their four F7 parental lines. Two widely grown F1 hybrids (Precos F1 and Addalyn F1) were used as controls. The results obtained for carbohydrates (HPLC-RID) indicated that the highest values (27.82 mg/g) were recorded in the paternal line AS 10 of the new hybrid AS 400 F1. The highest values of total organic acids (HPLC-VWD) were recorded in Adda

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18

Călugăr, Paul Cristian, Teodora Emilia Coldea, Carmen Rodica Pop, et al. "Mixed Fermentations of Yeasts and Lactic Acid Bacteria as Sustainable Processes to Enhance the Chemical Composition of Cider Made of Topaz and Red Topaz Apple Varieties." Agronomy 13, no. 10 (2023): 2485. http://dx.doi.org/10.3390/agronomy13102485.

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This study examined the effect of simultaneous fermentations of Saccharomyces cerevisiae and Pichia kluyveri, Lactobacillus plantarum and Oenococcus oeni on the chemical composition of apple cider from two apple varieties—Topaz and Red Topaz. Analytical techniques (HPLC-RID, HPLC-VWD, GC/MS, GC/FID, HPLC-DAD ESI+) were employed to analyze glucides, organic acids, volatile compounds, amino acids and phenolic compounds, respectively. Statistical analysis and and PCA were conducted to assess the correlations among samples based on the compounds identified. In the mixed fermentations, such as Sacc

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19

Elshewy, Ahmed, Federica Blando, Hammam Bahlol, Ahmed El-Desouky, Palmira De De Bellis, and Ibrahim Khalifa. "Egyptian Opuntia ficus-indica (OFI) Residues: Recovery and Characterization of Fresh Mucilage from Cladodes." Horticulturae 9, no. 7 (2023): 736. http://dx.doi.org/10.3390/horticulturae9070736.

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The utilization of biopolymers gained recent attention worldwide due to their effective role in producing eco-friendly and economical products. Mucilage from Opuntia ficus-indica (OFI, and other succulent plants) has the capacity to absorb huge amounts of water, forming a viscous colloid with interesting rheological properties. It also had the potential ability for use as food additives or food technological products. We, therefore, aimed at extracting and characterizing pure liquid mucilage from the cladodes of OFI (Egyptian variety) using multidimensional approaches including HPLC-RID, FTIR,

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20

Llano, Tamara, Natalia Quijorna, Ana Andrés, and Alberto Coz. "Sugar, acid and furfural quantification in a sulphite pulp mill: Feedstock, product and hydrolysate analysis by HPLC/RID." Biotechnology Reports 15 (September 2017): 75–83. http://dx.doi.org/10.1016/j.btre.2017.06.006.

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21

Liu, Hongxia, Shusheng Zhang, Ajuan Yu, et al. "Studies on intestinal permeability of cirrhotic patients by analysis lactulose and mannitol in urine with HPLC/RID/MS." Bioorganic & Medicinal Chemistry Letters 14, no. 9 (2004): 2339–44. http://dx.doi.org/10.1016/j.bmcl.2004.01.107.

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22

Luo, Xiaohu, Yu Liu, Jiali Xing, et al. "Comparison of ELSD and RID combined with HPLC for simultaneous determination of six rare sugars in food components." Microchemical Journal 201 (June 2024): 110666. http://dx.doi.org/10.1016/j.microc.2024.110666.

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23

Tiwari, Mrityunjay, Sandesh Mhatre, Tejas Vyas, Arohi Bapna, and Govindarajan Raghavan. "A Validated HPLC-RID Method for Quantification and Optimization of Total Sugars: Fructose, Glucose, Sucrose, and Lactose in Eggless Mayonnaise." Separations 10, no. 3 (2023): 199. http://dx.doi.org/10.3390/separations10030199.

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Mayonnaise is an oil-in-water emulsion containing 70–80% finely dispersed droplets of oil in a continuous phase of water. Since mayonnaise has a sour and acidic taste, its sugar profile is barely noticed and thus often disregarded. However, today, there are various variants of mayonnaise available on the market; hence, it is crucial to understand their mono- and disaccharide profile, in order to determine the precise total sugar composition. The traditional methods of sugar analysis available, such as titration, can only quantify sucrose and are unable to differentiate between mono- and disacc

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Rusinova-Videva, Snezhana, Manol Ognyanov, Yordan Georgiev, Margarita Kambourova, Aleksandar Adamov, and Vasilena Krasteva. "Production and Chemical Characterization of Exopolysaccharides by Antarctic Yeasts Vishniacozyma victoriae and Tremellomycetes sp." Applied Sciences 12, no. 4 (2022): 1805. http://dx.doi.org/10.3390/app12041805.

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The study aimed to investigate exopolysaccharides (EPSs) produced by two Antarctic yeasts isolated from Livingston Island. The species were identified as Vishniacozyma victoriae (V) and Tremellomycetes sp. (T) based on a molecular genetic analysis of ITS1-5.8S-ITS4 regions of the 18S rRNA gene. The EPS production was investigated under stress conditions in culture flasks and a bioreactor. Different chromatographic (HPLC-RID, HPSEC-RID) and spectral (FT-IR) analyses were employed to characterize EPSs. Tremellomycetes sp. accumulated 7 g/L biomass and 4.5 g/L EPS after 120 h of cultivation. The

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Chiş, Maria Simona, Adriana Păucean, Simona Maria Man, et al. "Quinoa Sourdough Fermented with Lactobacillus plantarum ATCC 8014 Designed for Gluten-Free Muffins—A Powerful Tool to Enhance Bioactive Compounds." Applied Sciences 10, no. 20 (2020): 7140. http://dx.doi.org/10.3390/app10207140.

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Lactobacillus plantarum ATCC 8014 was used to ferment quinoa flour, in order to evaluate its influence on the nutritional and rheological characteristics of both the sourdough and muffins. The quantification of carbohydrates and organic acids was carried out on a HPLC-RID system (high-performance liquid chromatography coupled with with refractive index detector), meanwhile HPLC-UV-VIS (high-performance liquid chromatography coupled with UV-VIS detector), AAS (Atomic absorption spectrophotometry), aluminum chloride colorimetric assay, Folin–Ciocalteu, and 1,1-Diphenyl-2-picrylhydrazyl radical s

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Atlı, Ercüment, İlknur Solmaz, Nebahat Sarı, and Haşim Kelebek. "Determination of Some Agronomic and Fruit Quality Characteristics of Some Watermelon Accessions from Turkish Watermelon Germplasm." Turkish Journal of Agriculture - Food Science and Technology 9, no. 8 (2021): 1322–28. http://dx.doi.org/10.24925/turjaf.v9i8.1322-1328.2672.

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This study has been conducted to determine the fruit quality parameters such as sugar and carotenoid content as well as plant and fruit characteristics of 11 local watermelon genotypes from watermelon genetic resources collection of Cukurova University, Department of Horticulture. First and 50% male and female flowering period, main stem length, main stem diameter, number of nodes on main stem, total yield, fruit weight, fruit length, fruit diameter, fruit rind thickness, total soluble solids (TSS), sugar and carotenoid contents were examined. Although there is no significant difference for ma

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C. Bhuvaneswari, C. Bhuvaneswari, and R. Sivasubramanian R. Sivasubramanian. "Phytochemical Analysis of Amorphophallus paeoniifolius (Dennst.) Nicolson and Its Standardisation by HPLC and HPTLC." Oriental Journal Of Chemistry 39, no. 1 (2023): 56–68. http://dx.doi.org/10.13005/ojc/390107.

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Elephant foot yams (Amorphophallus paeoniifolius (Denst.) Nicolson) are an important part of the diet in many parts of Asia. This study aims to analyse the physiochemical, phytochemicals, and in vitro antioxidant properties of different alcoholic and aqueous extracts. HPTLC and HPCL profile of ethanolic extract tubers of A. paeoniifolius was also investigated. Important secondary metabolites were detected through physicochemical analysis and phytochemical screening. In a laboratory, the antioxidant activity of A. paeoniifolius extracts was evaluated using standard free radical scavenging assay

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Yeganeh-Zare, Samal, Khalil Farhadi, and Saber Amiri. "Rapid detection of apple juice concentrate adulteration with date concentrate, fructose and glucose syrup using HPLC-RID incorporated with chemometric tools." Food Chemistry 370 (February 2022): 131015. http://dx.doi.org/10.1016/j.foodchem.2021.131015.

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Meng, Dong-shuo, Guo-jing Li, Shen-guo Sun, Chun-xin Ma, Tian-quan Zhu, and Yu Liu. "GC and HPLC-RID method development and validation for the determination of twelve related substances, including several novel compounds in tetrabenazine." Journal of Pharmaceutical and Biomedical Analysis 260 (August 2025): 116814. https://doi.org/10.1016/j.jpba.2025.116814.

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30

Vişan, Vasile-Gheorghe, Maria Simona Chiş, Adriana Păucean, et al. "Influence of Marination with Aromatic Herbs and Cold Pressed Oils on Black Angus Beef Meat." Foods 10, no. 9 (2021): 2012. http://dx.doi.org/10.3390/foods10092012.

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Beef aging is one of the most common methods used for improving its qualities. The main goal of the present study was to analyse the influence of different cold pressed oils and aromatic herbs during marination process on the nutritional, textural, and sensory attributes of the final grilled sirloin samples. In order to fulfil this goal, methods like GC-MS, HPLC/DAD/ESI-MS, HLPC-RID were performed to quantify fatty acids, phenolic acids, and organic acids, respectively. Textural and sensory analysis were performed with CT 3 Texture Analyser and hedonic test. The results showed high improvement

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Majore, Kristīne, and Inga Ciproviča. "Optimisation of Lactose Hydrolysis by Combining Solids and ß-Galactosidase Concentrations in Whey Permeates." Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences. 74, no. 4 (2020): 263–69. http://dx.doi.org/10.2478/prolas-2020-0041.

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AbstractA detailed study of lactose hydrolysis was conducted using 50, 250 and 500 units of ß-galactosidase (Kluyveromyces lactis and Bacillus licheniformis origin) in acid and sweet whey permeates at different solid concentrations 20%, 30% and 40% (w·v−1). The amount of lactose, glucose and galactose was measured by HPLC – RID. Hydrolysis was carried out at optimal enzyme temperature 42.5 °C for 4 h. Medium pH before hydrolysis was adjusted using 10% KOH. The experimental results were compared taking into account the sugar profiles and experimental conditions. The highest lactose hydrolysis o

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Ergen, Harun, Muge Guleli, Cigdem Sener, Cem Caliskan, Sercan Semiz, and Mahmut Ozbek. "Development and Validation of a Novel Reversed Phase High Performance Liquid Chromatography with Refractive Index Detector Method for Assay of Polyvinyl Alcohol in an Ophthalmic Solution." Current Pharmaceutical Analysis 16, no. 7 (2020): 867–71. http://dx.doi.org/10.2174/1573412915666190304152901.

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Introduction: Polyvinyl alcohol (PVA), a polymer, is in demand due to its usage in different applications such as pharmaceutical, biomedical and textile, paper, food industries. Methods: A new sensitive reversed phased high-pressure liquid chromatography (RP-HPLC) method with refractive index detector (RID) was developed for determination of PVA in an ophthalmic solution containing dexpanthenol and PVA as active substances and it was validated according to The International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH) guideline. Results: Chromatog

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Eliashvili, Levan. "Quantification of Glucose and Fructose in Georgian Semi-sweet Wines." Works of Georgian Technical University, no. 2(532) (June 10, 2024): 11–14. http://dx.doi.org/10.36073/1512-0996-2024-2-11-14.

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One of the important parameters for wine certification is the quantitative determination of glucose and fructose, for which the highly efficient liquid chromatographic method is tested and is accurate. The quantitative determination of glucose and fructose in semi-sweet wines using high performance liquid chromatography is discussed in the article. A high-performance liquid chromatograph (HPLC) with a refractive index detector (RID) and a reversed-phase alkali amine chromatography column was used during the analysis. The method showed a high correlation coefficient during calibration (R2 = 0.9

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Šeremet, Danijela, Petra Subotić, Ksenija Marković, et al. "Development, bioactive and sensory analysis of the honey-filled chocolate pralines infused with ground ivy (Glechoma hederacea L.) extract." Hrvatski časopis za prehrambenu tehnologiju, biotehnologiju i nutricionizam 18, no. 1-2 (2023): 49–54. http://dx.doi.org/10.31895/hcptbn.18.1-2.6.

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Chocolate is consumed largely worldwide and it is known as one of the most craved foods. The market of chocolate products is growing steadily and is expected to continue to grow in the coming years. The aim of the present study was to formulate chocolate pralines with enriched sensory and bioactive attributes by incorporating different types of honey (false indigo, buckwheat, rapeseed, mandarin and sage) and ground ivy (Glechoma hederacea L.) extract as ingredients of the filler. The honey samples were subjected to physico-chemical analysis, sugar analysis using the HPLC-RID methodology, as we

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Silva, Luciana, Sofia Orišková, Diogo Gonçalves, et al. "Quantification of Hydrolytic Sugars from Eucalyptus globulus Bio-Oil Aqueous Solution after Thermochemical Liquefaction." Forests 14, no. 4 (2023): 799. http://dx.doi.org/10.3390/f14040799.

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Eucalyptus globulus sawdust is a residue from the pulp and paper industry which has been underutilised and undervalued. The thermochemical liquefaction of sawdust can be considered an alternative for recycling this residue, as it results in the production of a bio-oil that, when extracted in water, allows the obtention of an aqueous solution composed of carbohydrates. The sugars resulting from the aqueous fraction of bio-oil can be valued by and applied in the industry to produce sustainable materials. For the first time, the sugar composition of the aqueous extract of bio-oil was disclosed, i

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Gou, Yan, Donglin Gu, Jing Fan, et al. "Purification and Structural Characterization of Polysaccharides from Polygonum multiflorum Thunb. and Their Immunostimulatory Activity in RAW264.7 Cells." Foods 13, no. 6 (2024): 932. http://dx.doi.org/10.3390/foods13060932.

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Polygonum multiflorum Thunb. (PM) and derived products are broadly utilized in Chinese traditional medicine. According to our previous research, PM mostly contains polysaccharides, which display a wide range of biological activities. Two water-soluble polysaccharides (PMPs-1 and PMPs-2) were obtained from PM by DEAE-Cellulose and Sephadex G-100 column chromatography. Colorimetry, HPGPC-MALLS-RID, HPLC-PDA, methylation, FT-IR, NMR, and SEM were used to characterize these polysaccharides. PMPs-1 and PMPs-2 had average molecular weights of 255.5 and 55.7 kDa, respectively. PMPs-1 consisted of Man

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Maha, Abdalla Alnuwaiser. "CONTENT OF SUGARS IN FRESH GRAPES AND RAISINS, AND FRESH AND DRIED APRICOT: A COMPARATIVE STUDY." International Journal of Research - Granthaalayah 5, no. 11 (2017): 177–86. https://doi.org/10.5281/zenodo.1095050.

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Sugars, mainly fructose, glucose, maltose and sucrose ratios in the fresh red grape and raisins and in the fresh and dried apricot fruit were determined by High Performance Liquid Chromatography, HPLC, equipped with Ec 250/4.6 Nucleodur 100-5 NH2-RP column and Refractometer -RID as a detector. Each type of the four samples was separately homogenized after removing the seeds from the samples before crushing, and in this study the deionized water was used for extraction of sugars. The ratio of each sugar in the four investigated cultivars was compared as well as comparing the contents of total s

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Yin, Xia, Chen Bin, Yanqing Wang, and Peng Xu. "Development and Validation of a Novel High-Performance Liquid Chromatography (HPLC)/Refractive Index Detector (RID) Method to Determine Residual Polyvinyl Alcohol (PVA) in Microspheres." Journal of Chemistry 2022 (October 4, 2022): 1–10. http://dx.doi.org/10.1155/2022/3563415.

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Polyvinyl alcohol (PVA) is a commonly used emulsifier for the preparation of DL-lactide-co-glycolide (PLGA) and polylactic acid (PLA) microspheres with sustained-release profile. Although water-soluble PVA is normally considered to have low toxicity, monitoring residual level of PVAs is still necessary, especially for the chronic disease treatment medications containing PVA. In this study, a robust and validated method was developed for the determination of PVA using high-performance liquid chromatography (HPLC) and refractive index detector (RID). PVA in sustained-release microspheres contain

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Yilmaz, Mustafa Tahsin, Nevruz Berna Tatlisu, Omer Said Toker, et al. "Steady, dynamic and creep rheological analysis as a novel approach to detect honey adulteration by fructose and saccharose syrups: Correlations with HPLC-RID results." Food Research International 64 (October 2014): 634–46. http://dx.doi.org/10.1016/j.foodres.2014.07.009.

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Alnuwaiser, MahaAbdalla. "CONTENT OF SUGARS IN FRESH GRAPES AND RAISINS, AND FRESH AND DRIED APRICOT: A COMPARATIVE STUDY." International Journal of Research -GRANTHAALAYAH 5, no. 11 (2017): 177–86. http://dx.doi.org/10.29121/granthaalayah.v5.i11.2017.2345.

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Sugars, mainly fructose, glucose, maltose and sucrose ratios in the fresh red grape and raisins and in the fresh and dried apricot fruit were determined by High Performance Liquid Chromatography, HPLC, equipped with Ec 250/4.6 Nucleodur 100-5 NH2-RP column and Refractometer -RID as a detector. Each type of the four samples was separately homogenized after removing the seeds from the samples before crushing, and in this study the deionized water was used for extraction of sugars. The ratio of each sugar in the four investigated cultivars was compared as well as comparing the contents of t

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Vasilica, Beldean (Tătar) Bianca, Maria Simona Chiș, Ersilia Alexa, et al. "The Impact of Insect Flour on Sourdough Fermentation-Fatty Acids, Amino-Acids, Minerals and Volatile Profile." Insects 13, no. 7 (2022): 576. http://dx.doi.org/10.3390/insects13070576.

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Acheta domesticus (L.1758) has been recently accepted by the European Union as a novel food, being the third insect that has been approved for human consumption. Nowadays, researchers’ attention is focused on exploiting new protein sustainable sources, and, therefore, insect flour has gained more and more interest. Organic acids, fatty acids, amino acids, aroma volatile compounds, and minerals were analyzed through HPLC-RID (High-performance liquid chromatography), GC-MS (Gas chromatography-mass spectrometry), LC-MS (Liquid chromatography–mass spectrometry), ITEX/GC-MS and AAS (Atomic Absorpti

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Sturza, Alina, Adriana Păucean, Maria Simona Chiș, et al. "Influence of Buckwheat and Buckwheat Sprouts Flours on the Nutritional and Textural Parameters of Wheat Buns." Applied Sciences 10, no. 22 (2020): 7969. http://dx.doi.org/10.3390/app10227969.

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In recent years, food products manufactured with buckwheat and sprouts flours have attracted widespread interest due to their high nutritional value with various health benefits, becoming more and more popular. The purpose of this study was to assesses the influence of buckwheat and sprouts flours on the nutritional, sensorial and textural characteristics on the final baked products. In order to achieve these goals, methods like HPLC-RID (High-Perfomance Liquid Chromatography with Refractive Index Detection), aluminum chloride colorimetric assay, Folin-Ciocalteu and 1,1-Diphenyl-2-picrylhydraz

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Zeaiter, Zahraa, Maria Elena Regonesi, Sofia Cavini, Massimo Labra, Guido Sello, and Patrizia Di Gennaro. "Extraction and Characterization of Inulin-Type Fructans from Artichoke Wastes and Their Effect on the Growth of Intestinal Bacteria Associated with Health." BioMed Research International 2019 (September 25, 2019): 1–8. http://dx.doi.org/10.1155/2019/1083952.

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Globe artichoke is an intriguing source of indigestible sugar polymers such as inulin-type fructans. In this study, the effect of ultrasound in combination with ethanol precipitation to enhance the extraction of long chain fructans from artichoke wastes has been evaluated. The inulin-type fructans content both from bracts and stems was measured using an enzymatic fructanase-based assay, while its average degree of polymerization (DP) was determined by HPLC-RID analysis. Results show that this method provides artichoke extracts with an inulin-type fructans content of 70% with an average DP betw

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Sri Rama Krishna Surapureddi, Kunta Ravindhranath, and Saritha Anthireddy. "An HPLC tool for process monitoring: rare sugar D- psicose and D- fructose contents during the production through an enzymatic path." International Journal of Research in Pharmaceutical Sciences 11, no. 1 (2020): 775–80. http://dx.doi.org/10.26452/ijrps.v11i1.1894.

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D-Psicose/allulose, a rare sugar, is an essential raw material in the pharmaceutical and food industries. It is scantly found in nature and to meet its demand in industries, D-Psicose is generated enzymatically using D-fructose as a substrate. In these conversations, it is important to monitor D-Psicose, in order to control the process, impurities, optimize the reaction time and reduce the process cost The available analytical methods have their limitations in quantifying D-psicose and D-fructose mixtures. Hence there is a need for the development of a routine, sensitive, quick and precise ana

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Uzlaşır, Türkan, Hatice Kübra Şaşmaz, and Haşim Kelebek. "Comparison of Extraction Techniques for Determining Bioactive Compounds and Antioxidant Activity of Spirulina platensis." Turkish Journal of Agriculture - Food Science and Technology 12, no. 4 (2024): 554–60. http://dx.doi.org/10.24925/turjaf.v12i4.554-560.6677.

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Spirulina platensis (S. platensis) is a high-nutrient blue-green algae that has been used as a food supplement for a long time. It contains carbohydrates, lipids, proteins, vitamins, minerals, and bioactive compounds essential for basic human nutrition. It is known to have anti-cancer, antioxidant, anti-inflammatory, neuroprotective, hepatoprotective, and hypocholesterolemic properties due to the bioactive compounds it contains. In this study, the effects of freeze-thawing, a rapid freezing (-20°C) and thawing (4°C) process, and ultrasonically assisted extraction techniques on the color, antio

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Alsaleem, Turki, Taghreed Alsaleem, Reham Al-Dhelaan, et al. "Evaluation of Ethanol Formation in Fruit Juices During Refrigerated Storage Time and Its Halal Status." International Journal of Halal Research 4, no. 1 (2022): 19–28. http://dx.doi.org/10.18517/ijhr.4.1.19-28.2022.

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Halal status of ethanol resulting from natural fermentation in food is controversial. According to the GSO standard (2538:2017), the maximum residue level (MRL) of ethanol in fruit juices is 0.1% (v/v). This study examined the effect of storage duration on the formation of ethanol and sugar content in fruit juices. A total of 49 different fruit juice samples (orange, apple, berry, pineapple, and a mixture of grapes and berries) were purchased from local supermarkets and analyzed for ethanol by HS-GC-FID and sugar content (glucose, fructose, and sucrose) by HPLC-RID. The samples were stored in

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Fidan, Hafize, Nadezhda Petkova, Tana Sapoundzhieva, and Engin Isik Abanoz. "CARBOHYDRATE CONTENT IN BULGARIAN AND TURKISH CAROB PODS AND THEIR PRODUCTS." CBU International Conference Proceedings 4 (September 26, 2016): 796–802. http://dx.doi.org/10.12955/cbup.v4.855.

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Carob, Ceratonia siliqua, is cultivated for ornamental and industrial purposes in many Mediterranean countries. This study assessed carob pulp and syrup, a rich source of carbohydrates and sugars, by evaluating content of reducing sugars and total sugars in carob pulp before extraction of syrups. We identified the sugar content before and after treatment by using thin-layer (TLC) and high performance liquid chromatography with refractive index detection (HPLC-RID). It was established that total sugars increased with extraction and heat treatment. Sucrose (34.2 g/100 g dry weight; dw), glucose

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Smetanková, Jana, Zuzana Hladíková, František Valach, et al. "Influence of aerobic and anaerobic conditions on the growth and metabolism of selected strains of Lactobacillus plantarum." Acta Chimica Slovaca 5, no. 2 (2012): 204–10. http://dx.doi.org/10.2478/v10188-012-0031-1.

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Abstract Three wild strains of Lactobacillus plantarum were investigated for their growth and ability to produce lactic acid, acetic acid and ethanol under aerobic and anaerobic conditions. They were tested at three different temperatures (30 °C, 37 °C and 45 °C). The growth of lactobacilli was studied by measuring optical density (OD) at λ = 600 nm and pH value at the following times. With increasing temperature difference of cell yield was observed. The final cell yield under aerobic conditions was higher. Organic acids and ethanol were analysed using an HPLC RID method. Formation of lactic

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Antoniou, Chrystalla, Marios C. Kyriacou, Angelos C. Kyratzis, and Youssef Rouphael. "Linking Colorimetric Variation with Non-Volatile and Volatile Components of Carob Flour." Foods 12, no. 13 (2023): 2556. http://dx.doi.org/10.3390/foods12132556.

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Chromatic variation was examined for its association with flour composition and quality. Carob samples from variable altitudes and genetic backgrounds were milled and assessed for colorimetric parameter L* (lightness) and analyzed for phenols, tannins, antioxidant capacity, soluble carbohydrates (HPLC-RID), organic acids and protein (IC-CD), and volatile organic compounds (VOCs; HS-SPME/GC-MS). Higher altitudes and grafted genotypes yielded lighter-colored flours of higher antioxidant potential, phenols, tannins, sucrose, and malic acid concentrations. VOCs were mainly acids, esters, aldehydes

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Moreira, Telma, Maria Margarida Mateus, Luís C. Duarte, and Maria Joana Neiva Correia. "Characterization of Liquefaction Products from Lignocellulosic and Aquatic Biomass." Biomass 5, no. 2 (2025): 36. https://doi.org/10.3390/biomass5020036.

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Biomass liquefaction is a promising thermochemical route to convert lignocellulosic residues into bio-oil. This study evaluates the liquefaction behavior of 13 biomasses with varying particle sizes (0.3–2.0 mm) and moisture contents (5–11%) under mild solvolysis conditions. High-performance liquid chromatography (HPLC-RID) and thermogravimetric analysis (TGA) were used to characterize bio-oil composition and biomass properties, respectively. Maximum conversion (72%) was achieved for Miscanthus, while Ulva lactuca reached only 23% due to its low carbohydrate content. Hemicellulose-rich feedstoc

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