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Journal articles on the topic 'HPLC; Teneligliptin; internal standard'

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Published: 3 June 2025
Last updated: 31 July 2025

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1

M.Anusha, *. and Nallakumar Ponnu Swamy. "BIOANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF TENELIGLIPTIN USING RP-HPLC IN RABBIT PLASMA." Indo American Journal of Pharmaceutical Sciences 04, no. 08 (2017): 2652–60. https://doi.org/10.5281/zenodo.883129.

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A simple, highly sensitive, precise and accurate high-performance liquid chromatographic (HPLC) method was developed and validated for the determination of teneligliptin in rabbit plasma samples. The chromatographic separation was achieved with a reverse phase column thermo C18 (4.6×100 mm, 5µ) and the mobile phase consisted of methanol and 5mm potassium phosphate buffer (60:40 v/v) at a flow rate of 1mL/min. Sitagliptin was used as an internal standard. The retention time of Teneligliptin and sitagliptin were found to be 3.9and 2.2min respectively. The calibration curve was linear (r2 > or
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Attimarad, Mahesh, Katharigatta Narayanaswamy Venugopala, Anroop Balachandran Nair, Nagaraja Sreeharsha, and Pran Kishore Deb. "Experimental Design Approach for Quantitative Expressions of Simultaneous Quantification of Two Binary Formulations Containing Remogliflozin and Gliptins by RP-HPLC." Separations 9, no. 2 (2022): 23. http://dx.doi.org/10.3390/separations9020023.

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The aim of this study was to develop a fast RP-HPLC method for simultaneous measurement of two antidiabetic formulations (vildagliptin + remogliflozin and teneligliptin + remogliflozin) under identical experimental conditions. Using the Box–Behnken approach and response surface design, the interaction and quadratic influence of three variable parameters, acetonitrile %, pH of the mobile phase, and flow rate, on resolution between the peaks were optimized. To forecast the resolution of peaks (2.7 and 6.5) for the three anti-diabetic medications, the design space with desirability function was u
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3

OHTAKA, Reiko, Masako MAEDA, Takeshi IWAGAMI, et al. "Precision of Internal Standard Method in HPLC Analysis." YAKUGAKU ZASSHI 123, no. 5 (2003): 349–55. http://dx.doi.org/10.1248/yakushi.123.349.

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Sharma, P. S., and P. H. Patel. "DEVELOPMENT AND VALIDATION OF ANALYTICAL METHOD FOR SIMULTANEOUS ESTIMATION OF TENELIGLIPTIN HYDROBROMIDE HYDRATE, PIOGLITAZONE HYDROCHLORIDE, AND METFORMIN HYDROCHLORIDE IN PHARMACEUTICAL DOSAGE FORM." RASAYAN Journal of Chemistry 17, no. 04 (2024): 1983–90. http://dx.doi.org/10.31788/rjc.2024.1749032.

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A simple, precise, and accurate RP-HPLC method was developed and validated for the simultaneous estimation of TENE, PIO, and MET in pharmaceutical formulations as per ICH guidelines. The analytical wavelength utilized was 254.8 nm with linearity ranges of 1-6 μg/mL (TENE), 0.75-4.5 μg/mL (PIO), and 25-150 μg/mL (MET). Good linearity was demonstrated by the regression equations and correlation coefficients (r2 > 0.9985). Percentage recovery studies at 80%, 100%, and 120% levels showed mean recoveries within 99.5-102.3% (TENE), 99.4-102.2% (PIO), and 99.2-100.4% (MET) with relative standard d
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Yeung, Hoi-Sze, Wing-Hong Ching, Shirley Sau-Ling Lai, Wai-On Lee, and Yiu-Tung Wong. "Quantitative Analysis of Closantel and Rafoxanide in Bovine and Ovine Muscles by High-Performance Liquid Chromatography with Fluorescence Detection." Journal of AOAC INTERNATIONAL 93, no. 5 (2010): 1672–77. http://dx.doi.org/10.1093/jaoac/93.5.1672.

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Abstract An HPLC method with a fluorescence detector (HPLC-FLD) was described for the quantitative determination of closantel and rafoxanide in bovine and ovine muscles. A structural analog closely related to rafoxanide, viz., N-[4-(4-chlorophenoxy) phenyl]-2-hydroxy-3,5-diiodobenzamide, was synthesized as an internal standard. Bovine and ovine muscles were extracted with acetonitrileacetone (60 + 40, v/v) followed by cleanup on mixed mode anionic exchange SPE cartridges. After evaporation and reconstitution with the mobile phase, the sample was analyzed by HPLC-FLD using internal standard cal
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Jones, P. M., and K. Brune. "Monitoring cyclosporine by HPLC with cyclosporin C as internal standard." Clinical Chemistry 39, no. 1 (1993): 168–69. http://dx.doi.org/10.1093/clinchem/39.1.168.

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7

Iqbal, Muzaffar, Nasr Y. Khalil, Amer M. Alanazi, and Khalid A. Al-Rashood. "A simple and sensitive high performance liquid chromatography assay with a fluorescence detector for determination of canagliflozin in human plasma." Analytical Methods 7, no. 7 (2015): 3028–35. http://dx.doi.org/10.1039/c5ay00074b.

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8

Chen, W. X., P. Y. Li, S. Wang, J. Dong, and J. Z. Li. "Serum cholesterol determined by liquid chromatography with 6-chlorostigmasterol as internal standard." Clinical Chemistry 39, no. 8 (1993): 1602–7. http://dx.doi.org/10.1093/clinchem/39.8.1602.

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Abstract We describe an accurate and precise method for determining serum cholesterol by high-performance liquid chromatography (HPLC). After addition of 6-chlorostigmasterol as internal standard, serum is treated with alcoholic potassium hydroxide. Subsequently the cholesterol and internal standard are extracted from the mixture into n-hexane and then derivatized to phenylurethanes for measurement by HPLC with ultraviolet detection. The effective chromatographic separation and the use of an appropriate internal standard make this procedure free from interferences by other serum sterols and pr
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Attya, Mohamed, Leonardo Di Donna, Fabio Mazzotti, Alessia Fazio, Bartolo Gabriele, and Giovanni Sindona. "Detection of ochratoxin A based on the use of its diastereoisomer as an internal standard." Anal. Methods 6, no. 15 (2014): 5610–14. http://dx.doi.org/10.1039/c4ay00414k.

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10

Turpeinen, Ursula, Helene Markkanen, Matti Välimäki, and Ulf-Håkan Stenman. "Determination of urinary free cortisol by HPLC." Clinical Chemistry 43, no. 8 (1997): 1386–91. http://dx.doi.org/10.1093/clinchem/43.8.1386.

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Abstract We here report a reversed-phase HPLC method for the determination of free cortisol in human urine, using methylprednisolone as the internal standard. Before chromatography, samples were extracted with a C18 solid-phase extraction column and the steroids were separated on a LiChrospher 100 C18 column with a mobile phase of methanol/acetonitrile/water (43/3/54 by vol). Linearity, precision, and accuracy of the method were established. The detection limit was 10 pmol of cortisol, and total CVs were <8%. With various solid-phase extraction columns the recovery of cortisol was 36–97
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11

Mallu, Useni Reddy, Venkateswara Rao Anna, and Bikshal Babu Kasimala. "DEVELOPMENT AND VALIDATION OF A SIMPLE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY–UV METHOD FOR THE DETERMINATION OF VINORELBINE - A CHEMOTHERAPEUTIC DRUG IN SPIKED HUMAN PLASMA." Asian Journal of Pharmaceutical and Clinical Research 12, no. 1 (2019): 369. http://dx.doi.org/10.22159/ajpcr.2018.v12i1.29399.

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Objective: Vinorelbine (VNRB) is a chemotherapeutic drug used to treat non-small cell lung cancer and breast cancer. Literature survey reveals that there are no reverse-phase high-performance liquid chromatography (RP-HPLC) methods reported for the estimation of VNRB in spiked human plasma. Hence, the present work aimed to develop a simple and efficient RP-HPLC method for the estimation of VNRB in human plasma.Methods: Specimen preparation for the measurement of VNRB was performed through liquid-liquid extraction using methanol as extracting solvent and reconstructed with mobile phase. Paclita
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Mallu, Useni Reddy, Venkateswara Rao Anna, and Bikshal Babu Kasimala. "DEVELOPMENT AND VALIDATION OF A SIMPLE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY–UV METHOD FOR THE DETERMINATION OF VINORELBINE - A CHEMOTHERAPEUTIC DRUG IN SPIKED HUMAN PLASMA." Asian Journal of Pharmaceutical and Clinical Research 12, no. 1 (2019): 369. http://dx.doi.org/10.22159/ajpcr.2019.v12i1.29399.

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Objective: Vinorelbine (VNRB) is a chemotherapeutic drug used to treat non-small cell lung cancer and breast cancer. Literature survey reveals that there are no reverse-phase high-performance liquid chromatography (RP-HPLC) methods reported for the estimation of VNRB in spiked human plasma. Hence, the present work aimed to develop a simple and efficient RP-HPLC method for the estimation of VNRB in human plasma.Methods: Specimen preparation for the measurement of VNRB was performed through liquid-liquid extraction using methanol as extracting solvent and reconstructed with mobile phase. Paclita
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13

Imre, Silvia, Amelia Tero-Vescan, Maria Titica Dogaru, et al. "With or Without Internal Standard in HPLC Bioanalysis. A Case Study." Journal of Chromatographic Science 57, no. 3 (2019): 243–48. http://dx.doi.org/10.1093/chromsci/bmy106.

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14

Sanches-Silva, Ana, José M. Cruz, Raquel Sendón-Garcĺa, and Perfecto Paseiro-Losada. "Determination of Butylated Hydroxytoluene in Food Samples by High-Performance Liquid Chromatography with Ultraviolet Detection and Gas Chromatography/Mass Spectrometry." Journal of AOAC INTERNATIONAL 90, no. 1 (2007): 277–83. http://dx.doi.org/10.1093/jaoac/90.1.277.

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Abstract A reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed and compared with a gas chromatography/mass spectrometry (GC/MS) method for determining butylated hydroxytoluene (BHT) in foodstuffs as a result of migration from plastic packaging. Similar extraction procedures were used in both methods. BHT was quantitated using an external standard in the HPLC method and an internal standard in the GC/MS method. Both methods presented good linearity (r2 ≥ 0.9917) and low detection limits. Recoveries obtained with the HPLC method (chicken meat, 95.8%, and Gouda ch
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15

Lin, Hongxia, Susan Goodin, Roger K. Strair, Robert S. DiPaola, and Murugesan K. Gounder. "Comparison of LC-MS Assay and HPLC Assay of Busulfan in Clinical Pharmacokinetics Studies." ISRN Analytical Chemistry 2012 (February 19, 2012): 1–5. http://dx.doi.org/10.5402/2012/198683.

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Busulfan is used in preparative regimens for bone marrow transplantation and timely busulfan plasma concentration reporting is critical for subsequent dose adjustment. We compared two sensitive methods for pharmacokinetics studies including LC-MS assay and HPLC precolumn derivatization assay. Chromatographic separation was performed on a Gemini C18 column. Liquid-liquid extraction with ethyl acetate was used for plasma sample preparation. Busulfan and internal standard ([2H8]-busulfan) were detected as ammonium adducts at m/z 264.2 and 272.2 for LC-MS assay. For HPLC assay, the extraction from
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16

Paroni, Rita, Barbara Comuzzi, Cinzia Arcelloni, et al. "Comparison of Capillary Electrophoresis with HPLC for Diagnosis of Factitious Hypoglycemia." Clinical Chemistry 46, no. 11 (2000): 1773–80. http://dx.doi.org/10.1093/clinchem/46.11.1773.

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Abstract Background: The diagnosis of “factitious hypoglycemia” is essentially based on the disclosure of hypoglycemic agents in blood or urine. The aim of this study was to evaluate the performance of capillary electrophoresis (CE) as a quantitative method for determination of chlorpropamide, tolbutamide, glipizide, gliclazide, and glibenclamide in serum. Methods: Serum samples (1 mL), with internal standard added, were purified by solid-phase extraction on OASISTM HLB cartridges (Waters), dried under reduced pressure, and reconstituted with 30–60 μL of acetonitrile:H2O. Analysis was carried
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17

Xie, Yuesheng, Dayong Zheng, Ting Yang, et al. "Head-to-Head Comparison of High-Performance Liquid Chromatography versus Nuclear Magnetic Resonance for the Quantitative Analysis of Carbohydrates in Yiqi Fumai Lyophilized Injection." Molecules 28, no. 2 (2023): 765. http://dx.doi.org/10.3390/molecules28020765.

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Carbohydrate analysis can be used as a standard analysis for quality control of industries of plants, foods and pharmaceuticals. Quantitative 1H NMR spectroscopy (qNMR) is an excellent alternative to chromatography-based mixture analysis. However, the application of qNMR in sugar analysis has rarely been reported. In this study, the performance of qNMR in sugar analysis was investigated and compared with the results from HPLC analysis. A head-to-head comparison of qNMR (internal and external standard methods) versus HPLC (PMP pre-column derivatization HPLC, HPLC-RID and HPLC-ELSD) based on qua
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18

Cabanes, A., Y. Cajal, I. Haro, J. M. Garcia Anton, F. Reig, and M. Arboix. "Gentamicin Determination in Biological Fluids by HPLC, Using Tobramycin as Internal Standard." Journal of Liquid Chromatography 14, no. 10 (1991): 1989–2010. http://dx.doi.org/10.1080/01483919108049669.

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19

Arsova-Sarafinovska, Zorica, Liljana Ugrinova, Katerina Starkoska, Dragan Djordjev, and Aneta Dimitrovska. "Determination of ethinylestradiol and levonorgestrel in oral contraceptives with HPLC methods with UV detection and UV/fluorescence detection." Macedonian Pharmaceutical Bulletin 52 (April 2006): 9–16. http://dx.doi.org/10.33320/maced.pharm.bull.2006.52.002.

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Oral contraceptives are pharmaceutical formulations containing an estrogen in a small amount and a synthetic progestin in 5-30 times bigger amount. A sensitive, accurate and rapid method for determination of active compounds is required. We have developed HPLC methods for determination of ethinylestradiol (EED) and levonorgestrel (LNG) in commercially available tablets. Chromatographic separation was performed on a Purospher® STAR RP-18e reversed-phase column (150 X 4.0 mm I.D.; particle size 5 µm) in an isocratic mode with a mobile phase constituted of 47% acetonitrile: 53% water (V/V) for bo
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20

Huang, Liusheng, Patricia S. Lizak, Anura L. Jayewardene, Florence Marzan, Ming-Na Tina Lee, and Francesca T. Aweeka. "A Modified Method for Determination of Lumefantrine in Human Plasma by HPLC-UV and Combination of Protein Precipitation and Solid-Phase Extraction: Application to a Pharmacokinetic Study." Analytical Chemistry Insights 5 (January 2010): ACI.S4431. http://dx.doi.org/10.4137/aci.s4431.

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An HPLC-UV method was developed and validated for the determination of lumefantrine in human plasma. Lumefantrine and its internal standard halofantrine were extracted from plasma samples using protein precipitation with acetonitrile (0.2% perchloric acid) followed by solid-phase extraction with Hypersep C8 cartridges. Chromatographic separation was performed on a Zorbax SB-CN HPLC column (3.0 × 150 mm, 3.5 μm) with water/methanol (0.1% TFA) as the mobile phases in a gradient elution mode. Detection was performed using UV/vis detector at λ = 335 nm. The method showed to be linear over a range
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21

Awni, W. M., and L. J. Bakker. "Antipyrine, indocyanine green, and lorazepam determined in plasma by high-pressure liquid chromatography." Clinical Chemistry 35, no. 10 (1989): 2124–26. http://dx.doi.org/10.1093/clinchem/35.10.2124.

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Abstract This HPLC method for measuring antipyrine, lorazepam, and indocyanine green in 0.5 mL of plasma can be used in studies of liver function in which these "model" compounds are used. After a fast, simple, one-step extraction procedure with acetonitrile, an isocratic HPLC system is used, with a single detection wavelength (214 nm) and a single internal standard (1-acetamidopyrene). The mobile phase is a 47/53 (by vol) mixture of acetonitrile and 50 mmol/L phosphate buffer, pH 6. The three compounds are separated on an LC-18 reversed-phase column. The low cost of the HPLC method makes feas
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22

Mazoit, Jean Xavier, Régine Le Guen, Anne Decaux, Pierre Albaladejo, and Kamran Samii. "Application of HPLC to counting of colored microspheres in determination of regional blood flow." American Journal of Physiology-Heart and Circulatory Physiology 274, no. 3 (1998): H1041—H1047. http://dx.doi.org/10.1152/ajpheart.1998.274.3.h1041.

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Colored microspheres have become popular compared with radioactive microspheres because they do not use radioactivity. However, they suffer from a much greater variability in their determination. We have developed a new method for assaying the dye using high-performance liquid chromatography (HPLC) with internal standard. This technique permits accurate determination of ≤400 spheres in rat blood, heart, kidney, liver, and brain with a relative error [coefficient of variation (CV)] <10%. To date, only three colors (white, yellow, and red) may be used because, of the five colors tested, one (
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23

Khrushchev, A. Y., E. R. Akmaev, V. O. Bondarenko, and A. E. Metlin. "Quantitative analysis by Raman spectroscopy using potassium hexacyanoferrate (III) as an internal standard." Agrarian science, no. 1 (March 7, 2020): 13–16. http://dx.doi.org/10.32634/0869-8155-2020-334-1-13-16.

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In this article we proposed a method for quantitative determination of active substance in pharmaceuticals via Raman scattering using potassium ferricyanide as internal standard. We approved this method in ketoprophenum quantitative determination in drug for veterinary use “Ketojekt”. The calculation of metrological requirements of procedures are established proposed method of calibration. Either we demonstrated the focalisation factor of laser beam affects on the method reproducibility. The method provides a precise, rapid, convenient to quantitative analysis that is more effective than the s
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24

Czauderna, M., J. Kowalczyk, M. Marounek, J. P. Michalski, and A. J. Rozbicka-Wieczorek. " A new internal standard for HPLC assay of conjugated linoleic acid in animal tissues and milk." Czech Journal of Animal Science 56, No. 1 (2011): 23–29. http://dx.doi.org/10.17221/336/2009-cjas.

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A new method for the quantification of underivatized conjugated linoleic acid (CLA) isomers and CLA-metabolites by silver ion liquid chromatography (Ag<sup>+</sup>-HPLC) with photodiode array detection (DAD) is described. Conjugated fatty acids (CFA) and sorbic acid as the internal standard (IS) were separated on two 5 μm Chrompac ChromSpher Lipids columns (250 × 4.6 mm). Biological samples were hydrolyzed with 1M KOH in methanol and 2M KOH in water at room temperature for 12 h. Hydrolyzates were acidified and the free fatty acids were extracted with dichloromethan
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25

Shelke, Rakesh U., and Dinesh D. Rishipathak. "Development and validation of a new bioanalytical method for quantification of CDK4/6 inhibitor in Spiked Human Plasma by HPLC-UV." Current Issues in Pharmacy and Medical Sciences 36, no. 2 (2023): 81–86. http://dx.doi.org/10.2478/cipms-2023-0014.

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Abstract A sensitive and accurate high performance liquid chromatography method utilizing ultraviolet/visible light detection (HPLC-UV) for the quantification of Ribociclib in Spiked Human Plasma by HPLC-UV was developed and validated. Ribociclib (RCB) and the internal standard (IS), Trifluridine, were first extracted from plasma samples by a simple Protein Precipitation extraction using Acetonitrile. Plasma concentration of RCB and internal standard were then analyzed by applying reversed phase chromatography using Orochem orosil C18 (4.6 mm × 250 mm, 5 μ) and elution with a isocratic mobile
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26

Koller, M. "Results for 74 substances tested for interference with determination of plasma catecholamines by "high-performance" liquid chromatography with electrochemical detection." Clinical Chemistry 34, no. 5 (1988): 947–49. http://dx.doi.org/10.1093/clinchem/34.5.947.

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Abstract Various catecholamine metabolites, catecholamine-related compounds, catechols, drugs, amines, and other nitrogen compounds were injected onto an HPLC system ("ClinRep Catecholamine-Plasma" assay kit with a reversed-phase C18 column) used for measuring catecholamines. None of the 74 substances tested co-eluted with any of the catecholamines--norepinephrine, epinephrine, or dopamine--or with the internal standard, 3,4-dihydroxybenzylamine.
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27

Dawson, C. M., T. W. M. Wang, S. J. Rainbow, and T. R. Tickner. "A Non-Extraction HPLC Method for the Simultaneous Determination of Serum Paracetamol and Salicylate." Annals of Clinical Biochemistry: International Journal of Laboratory Medicine 25, no. 6 (1988): 661–67. http://dx.doi.org/10.1177/000456328802500611.

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A direct injection HPLC method for the sumultaneous measurement of serum paracetamol and salicylate is described using a Pinkerton internal surface reversed-phase column with benzoic acid as internal standard. The method is linear to at least 1000 mg/L for both drugs and shows good precision at levels of 62–500 mg/L. None of the drugs tested for interference affected the quantitation of either drug. In patient samples, the values obtained with this method correlated well with those from enzymatic paracetamol and Trinder salicylate methods.
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28

O'Halloran, Sean, and Kenneth F. Ilett. "Evaluation of a Deuterium-Labeled Internal Standard for the Measurement of Sirolimus by High-Throughput HPLC Electrospray Ionization Tandem Mass Spectrometry." Clinical Chemistry 54, no. 8 (2008): 1386–89. http://dx.doi.org/10.1373/clinchem.2008.103952.

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Abstract Background: Matrix effects in HPLC–electrospray ionization–tandem mass spectrometry (HPLC-ESI-MS/MS)1 can cause differences in the ionization of an internal standard (IS) compared with the analyte of interest. Unless sample cleanup or chromatographic conditions eliminate or minimize ion suppression or enhancement, variability in interpatient matrices may cause erroneous results. A stable isotope-labeled IS can be used to minimize analytical interpatient variation. Methods: We used protein precipitation and HPLC-ESI-MS/MS to quantify sirolimus (SIR) with both desmethoxyrapamycin (DMR)
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29

Singh, A., C. L. Singh, S. Kumar, and M. Kumar. "DEVELOPMENT AND VALIDATION OF NEW RP–HPLC METHOD FOR DETERMINATION OF BESIFLOXACIN IN ANIMAL MODEL." INDIAN DRUGS 52, no. 01 (2015): 26–32. http://dx.doi.org/10.53879/id.52.01.10228.

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A sensitive and accurate reversed– phase high performance liquid chromatography (RP-HPLC) method with UV absorbance detection at 289 nm was developed and validated for the determination and quantification of besifloxacin (BSF) in rat plasma. Ofloxacin was used as an internal standard (IS). The sample was prepared by liquid extraction of BSF from plasma, using methanol and acetonitrile (70:30). The chromatographic separation was achieved with octadecylsilane (ODS-3), Hypersil® C18 column (250 mm×6mm×5μm). The chromatographic runtime was less than 5 minutes where the retention time of internal s
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Abonassif, Mohammed, Mohammed Hefnawy, Mohamed Kassem, and Gamal Mostafa. "Determination of donepezil hydrochloride in human plasma and pharmaceutical formulations by HPLC with fluorescence detection." Acta Pharmaceutica 61, no. 4 (2011): 403–13. http://dx.doi.org/10.2478/v10007-011-0035-1.

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Determination of donepezil hydrochloride in human plasma and pharmaceutical formulations by HPLC with fluorescence detectionA sensitive, isocratic reversed-phase high performance liquid chromatographic method involving fluorescence detection was developed for the determination of donepezil hydrochloride in tablets and in human plasma. Pindolol was used as an internal standard. Good chromatographic separation was achieved by using an analytical column C18. The system operated at room temperature using a mobile phase consisting of methanol, phosphate buffer (0.02 mol L-1) and triethyl amine (pH
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31

Kolesnikova, O. N., O. V. Fadeikina, O. B. Ustinnikova, R. A. Volkova, and A. A. Movsesyants. "Development and certification of reference standards for phenolic content in biologicals, based on comparison of results obtained by GLC, HPLC, spectrophotometric, and colorimetric methods." BIOpreparations. Prevention, Diagnosis, Treatment 21, no. 3 (2021): 193–99. http://dx.doi.org/10.30895/2221-996x-2021-21-3-193-199.

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Phenol is used as a preservative in a number of biological products. Methods that are used for quantitative determination of phenol differ a lot. Current requirements for accredited laboratories include continuous internal quality control. Reference standards with a certified content of the analyte are an effective metrological tool for ensuring such control. The aim of the study was to develop and certify reference standards for phenolic content in biological products, based on comparison of results obtained by GLC, HPLC, spectrophotometric, and colorimetric methods. Materials and methods: di
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32

Christians, U., K. O. Zimmer, K. Wonigeit, G. Maurer, and K. F. Sewing. "Liquid-chromatographic measurement of cyclosporin A and its metabolites in blood, bile, and urine." Clinical Chemistry 34, no. 1 (1988): 34–39. http://dx.doi.org/10.1093/clinchem/34.1.34.

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Abstract Using solid-phase extraction columns and "high-performance" liquid-chromatographic (HPLC) analysis, we could determine cyclosporin A and nine of its metabolites in blood, bile, and urine. To facilitate calculations of concentrations of cyclosporin A and its metabolites from the chromatograms, we used cyclosporin D as internal standard. For the HPLC analysis we used two sequential 250-mm analytical columns filled with reversed-phase octyl (C8) sorbent, eluting with a concave gradient of water, adjusted to pH 3.0 with phosphoric acid, and acetonitrile. Peaks were detected at 205 nm. For
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33

Mulder, Erik J., Alida Oosterloo-Duinkerken, George M. Anderson, Elisabeth GE De Vries, Ruud B. Minderaa, and Ido P. Kema. "Automated On-Line Solid-Phase Extraction Coupled with HPLC for Measurement of 5-Hydroxyindole-3-acetic Acid in Urine." Clinical Chemistry 51, no. 9 (2005): 1698–703. http://dx.doi.org/10.1373/clinchem.2005.050062.

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Abstract Background: Quantification of 5-hydroxyindole-3-acetic acid (5-HIAA) in urine is useful in diagnosing and monitoring of patients with carcinoid tumors and in the study of serotonin (5-hydroxytryptamine) metabolism in various disorders. We describe an automated method that incorporates on-line solid-phase extraction (SPE) and HPLC to measure urinary 5-HIAA. Methods: Automated prepurification of urine was accomplished with HySphere-resin GP SPE cartridges containing strong hydrophobic polystyrene resin. The analyte (5-HIAA) and internal standard [5-hydroxyindole-3-carboxylic acid (5-HIC
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Li, Hui, and Philip J. Kijak. "Development of a Quantitative Multiclass/Multiresidue Method for 21 Veterinary Drugs in Shrimp." Journal of AOAC INTERNATIONAL 94, no. 2 (2011): 394–406. http://dx.doi.org/10.1093/jaoac/94.2.394.

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Abstract A multiclass/multiresidue method has been developed and validated for the determination of 21 veterinary drug residues in shrimp, including sulfonamides (sulfadiazine, sulfamerazine, sulfamethazine, sulfachloropyridazine, sulfadimethoxine, and sulfaquinoxaline); tetracyclines (oxytetracycline, tetracycline, and chlortetracycline); (fluoro)quinolones (norfloxacin, ciprofloxacin, enrofloxacin, sarafloxacin, difloxacin, flumequine, oxolinic acid, and nalidixic acid); and cationic dyes (malachite green, gentian violet, leucomalachite green, and leucogentian violet), using HPLC/MS/MS. All
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35

Gao, Jianping, Ye Ma, Zhenhu Guo, et al. "Evaluating the Degradation Process of Collagen Sponge and Acellular Matrix Implants In Vivo Using the Standardized HPLC-MS/MS Method." Separations 10, no. 1 (2023): 47. http://dx.doi.org/10.3390/separations10010047.

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The purpose of this study was to establish a collagen determination method based on an isotope-labeled collagen peptide as an internal reference via high-performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS), and using the established method to evaluate the degradation process of collagen-based implants in vivo. The specific peptide (GPAGPQGPR) of bovine type I collagen was identified with an Orbitrap mass spectrometer. Then, the quantification method based on the peptide detection with HPLC-MS/MS was established and validated, and then further used to analyze the degradation
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36

Ghaly, M., I. E. Talat, A. Abeer, M. Mohamed, and A. W. Elmetwalli. "Isolation Of Anticancer Drug Taxol Producing Endophytic Fungus Alternaria Alternata By Using HPLC And LC- MS." American Journal of Clinical Pathology 154, Supplement_1 (2020): S137—S138. http://dx.doi.org/10.1093/ajcp/aqaa161.300.

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Abstract Introduction/Objective In the exploration of an alternative source, optimum and numerous solvents with different percentage were exploited to extract Taxol from plant palm branche. The fungal endophytes producing secondary metabolites that are effective against the human infections have fascinated many researchers across the world. Among these, the exploration for novel cancer therapeutic agents is of countless reputation due to upsurge in the number of cancer deaths, high worth of the drugs and the side effects concomitant with cancer treatments. Accordingly, in the current study, an
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Fayos, O., GF Barbero, M. Savirón, et al. "Synthesis of (±)-3,4-dimethoxybenzyl-4-methyloctanoate as a novel internal standard for capsinoid determination by HPLC-ESI-MS/MS(QTOF)." Open Chemistry 16, no. 1 (2018): 87–94. http://dx.doi.org/10.1515/chem-2018-0007.

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AbstractCapsinoids exhibit health-promoting properties and are therefore compounds of interest for medical and food sciences. They are minor compounds present in relatively high concentrations in only a few number of pepper cultivars. It is desirable to quantify capsinoids to provide selected cultivars with high capsinoid contents, which can then be employed as health food product. Quantifying low concentrations of capsinoids from pepper fruit requires a precise and selective analytical technique such as HPLC coupled to electrospray ionization - mass spectrometry, with development of an intern
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Awni, W. M., J. A. Maloney, and K. L. Heim-Duthoy. "Liquid-chromatographic determination of fleroxacin in serum and urine." Clinical Chemistry 34, no. 11 (1988): 2330–32. http://dx.doi.org/10.1093/clinchem/34.11.2330.

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Abstract This highly sensitive, accurate, and reproducible HPLC method for determining fleroxacin in human serum and urine makes use of a common C18 column, a fluorescence detector, and an internal standard. Serum samples require a simple extraction procedure; urine must be diluted. The method, which we have used extensively for pharmacokinetic assessment of fleroxacin in patients, measures concentrations as low as 5 micrograms/L.
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39

Drzymala, S., J. Riedel, R. Köppen, L. A. Garbe, and M. Koch. "Preparation of 13C-labelled cis-zearalenone and its application as internal standard in stable isotope dilution analysis." World Mycotoxin Journal 7, no. 1 (2014): 45–52. http://dx.doi.org/10.3920/wmj2013.1610.

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Pure U-[13C18]-labelled cis-zearalenone (cis-ZEA) has been prepared and characterised as internal standard (ISTD) for a reliable quantification of cis-ZEA in contaminated food and feed products. The cis-isomer of the naturally trans-configurated Fusarium mycotoxin zearalenone is often neglected. However, isomerisation easily occurs by exposure of ZEA to (UV-)light. Thus, the applicability of the new cis-ZEA ISTD was demonstrated in a long-term isomerisation study comparing naturally trans-ZEA-contaminated edible oil with spiked edible oil. To estimate the benefits of the newly prepared cis-ZEA
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Xiong, Xin, and Suodi Zhai. "High-Performance Liquid Chromatography/Tandem Mass Spectrometry Method for the Determination of Arbidol in Human Plasma." Journal of AOAC INTERNATIONAL 94, no. 4 (2011): 1100–1105. http://dx.doi.org/10.1093/jaoac/94.4.1100.

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Abstract An HPLC/MS/MS method for the determination of arbidol in human plasma was developed. Arbidol and internal standard (loratadine) were extracted from alkaline plasma with tert-butyl methyl ether and analyzed on a Zorbax SB C18 column (30 × 2.1 mm id, 3.5 µm particle size). The detection was by monitoring arbidol at m/z 479.1 → 434.1 and the internal standard at m/z 383.2 → 337.2. The method was validated according to U.S. Food and Drug Administration guidelines. The calibration curve was linear over the range of 0.5–500 ng/mL using a 100 µL sample volume. The intraday and interday preci
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Zhang, Mei, Grant A. Moore, Murray L. Barclay, and Evan J. Begg. "A Simple High-Performance Liquid Chromatography Method for Simultaneous Determination of Three Triazole Antifungals in Human Plasma." Antimicrobial Agents and Chemotherapy 57, no. 1 (2012): 484–89. http://dx.doi.org/10.1128/aac.00768-12.

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ABSTRACTA rapid and simple high-performance liquid chromatography (HPLC) assay was developed for the simultaneous determination of three triazole antifungals (voriconazole, posaconazole, and itraconazole and the metabolite of itraconazole, hydroxyitraconazole) in human plasma. Sample preparation involved a simple one-step protein precipitation with 1.0 M perchloric acid and methanol. After centrifugation, the supernatant was injected directly into the HPLC system. Voriconazole, posaconazole, itraconazole, its metabolite hydroxyitraconazole, and the internal standard naproxen were resolved on a
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Bhusarapu, Satya Prasad, and Jaya Kumari Sekharan. "Estimation of Eravacycline Dihydrochloride in Biological Matrices by LC-MS/MS." Pharmaceutical Methods 10, no. 2 (2019): 6. https://doi.org/10.5281/zenodo.14586110.

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Objective: The validated protein precipitation method was applied for estimation of Eravacycline dihydrochloride in human plasma with Rolitetracycline hydrochloride as an internal standard (ISTD) by using HPLC-ESI-MS/ MS. Methods: The chromatographic separation was achieved with 20mM Ammonium acetate (pH-3.0):Methanol:Acetontrile (20:20:60,%v/v) using the TELOS LU C18 (2) 5µm, 100 x 4.6 mm. The total analysis time was 3 min and flow rate was set to 0.5 mL/min. Results: The mass transitions of Eravacycline dihydrochloride and Rolitetracycline hydrochloride obtained were m/z 632.5®84.3
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AlThikrallah, Maymonah K. I., Abubakr M. Idris, Abdalla Ahmed Elbashir, Rafea E. E. Elgorashe, Alyah Buzid, and Ahmed O. Alnajjar. "Development of Capillary Zone Electrophoresis Method for the Simultaneous Separation and Quantification of Metformin and Pioglitazone in Dosage Forms; and Comparison with HPLC Method." Molecules 28, no. 3 (2023): 1184. http://dx.doi.org/10.3390/molecules28031184.

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A capillary zone electrophoretic (CZE) method was developed, validated, and applied for the assay of metformin (MET) and pioglitazone (PIO) in pharmaceutical formulations. The optimum running buffer composition was found to be 75 mmol/L phosphate buffer containing 30% acetonitrile (ACN) at pH 4.0. The optimum instrumental conditions were found to be injection time, 10 s; applied voltage, 25 kV; hydrodynamic injection pressure, 0.5 psi for 10 sec, capillary temperature, 25 °C; and the detection wavelength, 210 nm. The quantifications were calculated based on the ratio of the peak areas of analy
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44

Yang, Qiao. "MAE-HPLC Determination of Bioactive Compounds in Gardenia jasminoides Ellis." Advanced Materials Research 1073-1076 (December 2014): 122–26. http://dx.doi.org/10.4028/www.scientific.net/amr.1073-1076.122.

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In this study, a novel microwave-assisted extraction (MAE) method has been developed for the extraction and determination of the major biologically active compounds, crocin and its seven trans-cis analogues in a traditional Chinese medicine (TCM), gardenia fruits (Gardenia jasminoides Ellis) with analysis by reversed phase HPLC. Sudan I [1-phenylazo-2-naphthol] was selected as the internal standard. The results of quantitative determination of seven crocin analogues in six commercial gardenia fruits samples show that MAE–HPLC is a simple, rapid, low cost and reliable method for the determinati
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YAMAGUCHI, Yuichi, Mari YAMAMOTO-MAEDA, and Kenkou TSUJI. "HPLC Analysis of Catechins and Caffeine in Tea Extract with Catechol as an Internal Standard." Chagyo Kenkyu Hokoku (Tea Research Journal), no. 84 (1997): 32–34. http://dx.doi.org/10.5979/cha.1997.32.

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46

Venkata, Kiran B., Sreenivas Rao Battula, and Somshankar Dubey. "Validation of Quetiapine Fumarate in Pharmaceutical Dosage by Reverse-Phase HPLC with Internal Standard Method." Journal of Chemistry 2013 (2013): 1–8. http://dx.doi.org/10.1155/2013/578537.

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A rapid, specific, and accurate isocratic HPLC method was developed and validated for the assay of quetiapine fumarate in pharmaceutical dosage forms. The assay involved an isocratic-elution of quetiapine fumarate in Grace C18 column using mobile-phase composition of 0.1% ortho phosphoric acid with triethyl amine as modifier buffer and acetonitrile in the ratio of 50 : 50 (v/v).The wavelength of detection is 294 nm. The method showed good linearity in the range of 2.0–50.2 × 10−3 g/Lt. The runtime of the method is 5 mins. The developed method was applied to directly and easily analyse of the p
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47

Cseh, Edina Katalin, Gábor Veres, Márton Szentirmai, et al. "HPLC method for the assessment of tryptophan metabolism utilizing separate internal standard for each detector." Analytical Biochemistry 574 (June 2019): 7–14. http://dx.doi.org/10.1016/j.ab.2019.03.005.

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48

Hui, Shu-Ping, Tsuyoshi Murai, Teruki Yoshimura, Hitoshi Chiba, Hironori Nagasaka, and Takao Kurosawa. "Improved HPLC assay for lipid peroxides in human plasma using the internal standard of hydroperoxide." Lipids 40, no. 5 (2005): 515–22. http://dx.doi.org/10.1007/s11745-005-1412-2.

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49

Sobhi, Khaled, Souhila Mélaïne, Abderrahmane Abdaoui, and Mohamed Mammar. "Toxicological screening method by HPLC-DAD with local spectral library and "home-made" internal standard." Journal of Fundamental and Applied Sciences 14, no. 2 (2023): 351–67. http://dx.doi.org/10.4314/jfas.1180.

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A liquid-liquid extraction technique and HPLC-DAD method using a C18 column, 5μm (150x4.6mm i.d.) were developed for a toxicological drug screening. A home-made “N-acetylparoxetine” is used as internal standard. A local UV spectra library of over 130 drugs and some of their metabolites was created. This library covers the main therapeutic classes involved in intoxication cases. The developed procedure is successfully applied to different biological samples (serum, urine, gastric fluid and post mortem whole blood) and allows rapid and simultaneous detection and identification of a large number
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Ziaei, Elham, Jaber Emami, Moloud Kazemi, and Mahboubeh Rezazadeh. "Simultaneous Determination of Docetaxel and Celecoxib in Porous Microparticles and Rat Plasma by Liquid-Liquid Extraction and HPLC with UV Detection: in vitro and in vivo Validation and Application." Journal of Pharmacy & Pharmaceutical Sciences 23 (August 7, 2020): 289–303. http://dx.doi.org/10.18433/jpps30912.

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Purpose: A simple, rapid, sensitive, and reliable HPLC method with UV detection was developed and validated for simultaneous quantitation of docetaxel and celecoxib and paclitaxel for dissolution characterization and pharmacokinetic studies. Methods: The HPLC assay was performed isocratically on a reversed-phase C18 μ-Bondapack column using a mobile phase of acetonitrile:water (45:55, v/v) at a flow rate of 1.2 mL/min, and the analytes were detected at 230 nm. Paclitaxel was used as an internal standard for analysis of plasma samples following simple liquid-liquid extraction with n-hexane:isoa
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