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Journal articles on the topic 'HPLC-UV detector'

Author: Grafiati

Published: 5 June 2025

Last updated: 1 August 2025

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1

Soliman, Karim, Feras Jirjees, Rahul Sonawane, et al. "Latanoprost Quantification in Ocular Implants and Tissues: HPLC-Fluorescence vs HPLC-UV." Journal of Chromatographic Science 59, no. 1 (2020): 64–70. http://dx.doi.org/10.1093/chromsci/bmaa078.

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Abstract Anti-glaucoma latanoprost-loaded ocular implants provide prolonged delivery and enhanced bioavailability relative to the conventional eye drops. This study aims at the development and validation of a reversed-phase high-performance liquid chromatography method for quantitative analysis of nanogram levels of latanoprost in the eye, and for the first time, compares the use of fluorescence vs ultraviolet (UV) detectors in latanoprost quantification. The mobile phase was composed of acetonitrile:0.1% v/v formic acid (60:40, v/v) with a flow rate of 1 mL/min and separation was done using a

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2

Ramya, Sudha P., P. Bharath, and D. Ramachandran. "A validated RP-HPLC method for quantitative determination of genotoxic impurity hydrazine content in Ursodeoxycholic acid." Research Journal of Chemistry and Environment 29, no. 5 (2025): 160–68. https://doi.org/10.25303/295rjce1600168.

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A highly sensitive method for the determination of genotoxic impurity such as hydrazine in Ursodeoxycholic acid using RP-HPLC has been presented in the present study. Quantification of hydrazine content in Ursodeoxycholic acid sample by HPLC was done with UV Detector. Hydrazine was UV inactive compound. Derivatization procedure was established to detect the hydrazine in HPLC. For this, 4-Hydroxy 3-methoxybenzaldehyde was used as a derivatizing agent which reacts with hydrazine in the presence of disodium tetraborate to form a compound which was UV active. Hydrazine was determined by RP-HPLLC m

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3

Schmid, Stefan, Mirek Macka, and Peter C. Hauser. "Deep-UV Detector for HPLC with Light-Emitting Diode." CHIMIA International Journal for Chemistry 62, no. 10 (2008): 860. http://dx.doi.org/10.2533/chimia.2008.860.

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4

Zhu, Yanqin, Qinhong Yin, and Yaling Yang. "A comparative study of HPLC-DAD and UPLC-UV methods for simultaneous determination of 11 polyphenols in Moringa oleifera leaves." Tropical Journal of Pharmaceutical Research 20, no. 11 (2021): 2371–79. http://dx.doi.org/10.4314/tjpr.v20i11.20.

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Purpose: To develop, validate and compare two chromatographic methods - high performance liquid chromatography with diode array detector ((HPLC-DAD) and high performance liquid chromatography with ultraviolet detection (UPLC-UV) for the effective analysis of polyphenols in Moringa oleifera leaves.Methods: HPLC-DAD and UPLC-UV methods were applied for the accurate determination of eleven major polyphenols in Moringa oleifera leaves. The chromatographic conditions of the eleven polyphenols was determined on two C18 column by gradient elution with 0.5 % phosphoric acid solution -acetonitrile as t

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5

Chillar, Komal, Yipeng Yin, Adikari M. Dhananjani N. Eriyagama, and Shiyue Fang. "Determination of optical density (OD) of oligodeoxynucleotide from HPLC peak area." PeerJ Analytical Chemistry 4 (July 7, 2022): e20. http://dx.doi.org/10.7717/peerj-achem.20.

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Oligodeoxynucleotides (ODNs) are typically purified and analysed with HPLC equipped with a UV-Vis detector. Quantities of ODNs are usually determined using a UV-Vis spectrometer separately after HPLC, and are reported as optical density at 260 nm (OD260). Here, we describe a method for direct determination of OD260 of ODNs using the area of the peaks in HPLC profiles. It is expected that the method will save significant time for researchers in the area of nucleic acid research, and minimize the loss of oligonucleotide samples.

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6

Simranjeet, Singh Muskan Sood Anu Jindal* Rajmeet Singh Satvinder Kaur Jaswinder Singh Shaveta Bhardwaj. "Analytical Method Validation of Tablet Dosage Form of Lurasidone HCl." International Journal in Pharmaceutical Sciences 1, no. 11 (2023): 240–45. https://doi.org/10.5281/zenodo.10112998.

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This research is mainly focused on development of an Excellent Gradient method by reverse phase - high performance liquid chromatography (RP-HPLC) using UV Visible Detector. The main objective of method validation of Lurasidone HCl as a tablet dosage form is to validate the method as in-house method. As this tablet dosage form is not registered in any of Pharmacopeia, so the method is developed and validated for further studies. The sample is analyzed by RP-HPLC using octadecylsilane (C18) column (Inertsil LC-GC) as stationary phase with UV-Visible detector. The 'Gradient Method' is used as in

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7

Diwesh Chawla, Khushbu Kumari, and Meera Sikka. "An improved and sensitive method for nitrotyrosine estimation by reverse phase-high performance liquid chromatography." GSC Biological and Pharmaceutical Sciences 23, no. 1 (2023): 089–95. http://dx.doi.org/10.30574/gscbps.2023.22.1.0014.

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Reactive nitrogen species such as peroxynitrite anion (ONOO-), nitrogen oxide radical (NO.) may lead to nitrotyrosine formation by oxidative nitration of tyrosine. Nitrotyrosine is considered as an important marker to estimate the severity of biological oxidative damage. Therefore, in the present study, we evaluated a reverse phase-high performance liquid chromatography (RP-HPLC) method to improve the sensitivity and specificity of the nitrotyrosine estimation. The mobile phase used in the present study was methanol (pH 4.7) and 0.1% phosphoric acid (60:40%). The standard serial dilutions were

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8

YADAV, SHIVANAND, and NEERAJ SHARMA. "DEVELOPMENT OF REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR SIMULTANEOUS ESTIMATION OF SILDENAFIL CITRATE AND DEPOXETINE HYDROCHLORIDE IN PHARMACEUTICAL FORMULATION." Current Research in Pharmaceutical Sciences 12, no. 1 (2022): 28–31. http://dx.doi.org/10.24092/crps.2022.120104.

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Simple, accurate and precise reversed-phase high-performance liquid chromatographic (RP-HPLC) methods for simultaneous estimation of sildenafil citrate (SIL) and depoxetine hydrochloride (DAP) in combined tablet dosage form have been developed and validated. The RP-HPLC method uses a Shimadzu – 1800, Software Version – UV Prob 2.33 with BDS hypersil C18 column and mixture of Buffer pH 4.0 and ACN in the ratio of 40:60 as the mobile phase. The detection was carried out using a UV–Visible Detector: Shimadzu SPD–20AT diode array detector set at 229 nm. Linearity of chromatographic method was foun

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Godiņa, Daniela, Raimonds Makars, Rudolfs Berzins, Aigars Paze, and Janis Rizhikovs. "Method Development for Leachable Furfural Determination in Wood-Based Panels by HPLC-Uv System." Key Engineering Materials 903 (November 10, 2021): 223–28. http://dx.doi.org/10.4028/www.scientific.net/kem.903.223.

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Analytical method has been developed and validated to determine free or leachable furfural concentration in wood-based panels. Particleboards obtained from birch wood and suberinic acids binder were chosen as a reference material. Two methods and two solvents were tested. Acetone extracts of the samples were analyzed with gas chromatography (GC) flame ionization detector system. Water extracts were analyzed with high-performance liquid chromatography-ultraviolet spectroscopy (HPLC-UV) system. After the GC data in acetone extracts furfural concentration was below method limit of detection. HPLC

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10

Dr., A. Madhukar Shradha Rajesh Mahajan Vaishnavi Rameshrao Siral Ravindranath Bhagwan Rathod Bhagwan Shesharao Ingole. "METHOD DEVELOPMENT AND VALIDATION OF SAXAGLIPTIN BY USING UV SPECTROPHOTOMETRIC AND RP-HPLC TECHNIQUES IN BULK AND TABLET DOSAGE FORM." Journal of Pharma Research 12, no. 02 (2023): 15–24. https://doi.org/10.5281/zenodo.8094571.

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<strong><em>ABSTRACT</em></strong> <strong>S</strong>imple, precise, economical, fast and reliable UV and RP- HPLC Techniques have been developed for the estimation of Saxagliptin in bulk and pharmaceutical dosage form. UV method is based on measurement of absorption at maximum wavelength of 281 nm for Saxagliptin. Linearity for detector response was observed in the concentration range of 1 - 18μg/ml. The accuracy of the methods was assessed by recovery studies and was found to be 98.26 to 101.143 %. Separation was achieved with an Inertsil ‐ Extend ‐ C18 (250 × 4.6 mm, 5 µm) HP

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Janeček, M., F. Foret, K. Šlais, and P. Boček. "A simple on-line fibre optics UV detector for microcolumn HPLC." Chromatographia 25, no. 9 (1988): 815–16. http://dx.doi.org/10.1007/bf02262091.

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12

Schmid, Stefan, Mirek Macka, and Peter C. Hauser. "UV-absorbance detector for HPLC based on a light-emitting diode." Analyst 133, no. 4 (2008): 465. http://dx.doi.org/10.1039/b715681b.

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13

Dillip Kumar Mohapatra, Rajesh K Nayak, Deepika Rani Panigrahi, and Surya Narayan Das. "Validation of Finasteride Tablets USP by HPLC Isocratic with UV Detector." International Journal of Scientific Research in Science and Technology 12, no. 1 (2025): 106–13. https://doi.org/10.32628/ijsrst25121163.

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The aims of this work were to validate the analytical method, and evaluate the fenasteride in bulk drug commercial products by HPLC. Fenasteride was eluted from a Kromasil ODS C18, 150 mm x 4.6 mm, 5 at laboratory temperature (30 ± 2º C) with a mobile phase consisting of Acetonitrile and 2.5M Ortho phosphoric acid (50:50 %v/v) at a flow rate of 1.0 mL/min with UV detection at 240 nm. The retention time was about 3.45 minutes and each analysis took not more than 8 minutes. The result obtained for each parameter including Specificity, System Precision, Method Precision (Repeatability), Intermed

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14

Resende, Michele F., Mellina D. R. Santos, Renato C. Matos, and Maria A. C. Matos. "The analysis of faecal sterols in sediment samples by HPLC-UV using ultrasound-assisted treatment." Anal. Methods 6, no. 24 (2014): 9581–87. http://dx.doi.org/10.1039/c4ay01964d.

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15

Amin, Elham, Yan-Hong Wang, Bharathi Avula, et al. "Simultaneous Determination of Saponins and a Flavonoid from Aerial Parts of Zygophyllum coccineum L." Journal of AOAC INTERNATIONAL 95, no. 3 (2012): 757–62. http://dx.doi.org/10.5740/jaoacint.11-216.

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Abstract Triterpenoid saponins are a class of glycosides with a wide range of bioactivities, which make them interesting research candidates. Zygophyllum coccineum is an Egyptian desert plant rich in triterpenoid saponins. Reviewing the relevant literature, no data concerning the HPLC or ultra-performance LC (UPLC) analysis of Zygophyllum content were found. This paper presents two methods, HPLC-UV and UPLC-UV-evaporative light scattering detector (ELSD)/MS, for the simultaneous determination of 10 compounds in the alcohol extract of Z. coccineum. The HPLC method uses a C18 column and water–ac

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Diwesh, Chawla, Kumari Khushbu, and Sikka Meera. "An improved and sensitive method for nitrotyrosine estimation by reverse phase-high performance liquid chromatography." GSC Biological and Pharmaceutical Sciences 22, no. 1 (2023): 089–95. https://doi.org/10.5281/zenodo.7654366.

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Reactive nitrogen species such as peroxynitrite anion (ONOO<sup>-</sup>), nitrogen oxide radical (NO.) may lead to nitrotyrosine formation by oxidative nitration of tyrosine. Nitrotyrosine is considered as an important marker to estimate the severity of biological oxidative damage. Therefore, in the present study, we evaluated a reverse phase-high performance liquid chromatography (RP-HPLC) method to improve the sensitivity and specificity of the nitrotyrosine estimation. The mobile phase used in the present study was methanol (pH 4.7) and 0.1% phosphoric acid (60:40%). The standard serial dil

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17

Shang, Xiaojun, Suying Ma, and Zheshen Li. "Development and Validation of a RP-HPLC Method for Determination of Nimodipine in Sustained Release Tablets." Journal of Chemistry 2013 (2013): 1–4. http://dx.doi.org/10.1155/2013/612082.

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A rapid, sensitive, and reproducible reverse phase high performance liquid chromatographic (RP-HPLC) method with UV detector for the determination of nimodipine in sustained release tablets was developed. The method involved using a SinoChoom ODS-BP C18reversed phase column (5 μm, 4.6 mm × 200 mm) and mobile phase consisting of methanol-acetonitrile-water (35 : 38 : 27, v/v). The flow rate is 1.0 mL/min, the UV detector was operated at 237 nm, and the column was maintained at 25°C. The method was validated according to official compendia guidelines. The calibration curve of nimodipine for RP-H

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Arsova-Sarafinovska, Zorica, Liljana Ugrinova, Katerina Starkoska, Dragan Djordjev, and Aneta Dimitrovska. "Determination of ethinylestradiol and levonorgestrel in oral contraceptives with HPLC methods with UV detection and UV/fluorescence detection." Macedonian Pharmaceutical Bulletin 52 (April 2006): 9–16. http://dx.doi.org/10.33320/maced.pharm.bull.2006.52.002.

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Oral contraceptives are pharmaceutical formulations containing an estrogen in a small amount and a synthetic progestin in 5-30 times bigger amount. A sensitive, accurate and rapid method for determination of active compounds is required. We have developed HPLC methods for determination of ethinylestradiol (EED) and levonorgestrel (LNG) in commercially available tablets. Chromatographic separation was performed on a Purospher® STAR RP-18e reversed-phase column (150 X 4.0 mm I.D.; particle size 5 µm) in an isocratic mode with a mobile phase constituted of 47% acetonitrile: 53% water (V/V) for bo

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19

Simranjeet, Singh Muskan Sood Anu Jindal* Rajmeet Singh Satvinder Kaur Jaswinder Singh Shaveta Bhardwaj. "Analytical Method Validation of Tablet Dosage Form of Lurasidone HCl." International Journal in Pharmaceutical Sciences 1, no. 11 (2023): 240–45. https://doi.org/10.5281/zenodo.10113218.

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This research is mainly focused on development of an Excellent Gradient method by reverse phase - high performance liquid chromatography (RP-HPLC) using UV Visible Detector. The main objective of method validation of Lurasidone HCl as a tablet dosage form is to validate the method as in-house method. As this tablet dosage form is not registered in any of Pharmacopeia, so the method is developed and validated for further studies. The sample is analyzed by RP-HPLC using octadecylsilane (C18) column (Inertsil LC-GC) as stationary phase with UV-Visible detector. The 'Gradient Method

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Kumar, S. Senthil, Ritesh Kumar Srivastava, and V. Srinivasrao. "DETERMINATION OF 4, 4′-BIS (BROMOMETHYL) BIPHENYL GENOTOXIC IMPURITY IN VALSARTAN DRUG SUBSTANCES BY HPLC." International Journal of Pharmacy and Pharmaceutical Sciences 8, no. 11 (2016): 209. http://dx.doi.org/10.22159/ijpps.2016v8i11.14614.

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Objective: The objective of the present study was to develop and validate a specific and sensitive analytical method, which separate the genotoxic impurity 4, 4’-bis (bromomethyl) biphenyl from valsartan antihypertensive drug substance using HPLC method.Methods: The development activity was conducted by HPLC with UV detector. The impurity was separated on Inertsil ODS 3V 250 x 4.6 mm, 5 µm analytical column with a mobile phase consisting of 5.5 pH buffer and acetonitrile with the gradient program at a flow rate 1.0 ml/min. The effluent was detected using UV detector attached with HPLC system a

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Bollepaka, Srilatha Santhosh Illendula. "STABILITY INDICATING METHOD DEVELOPMENT OF RIMONABANT AND IT's VALIDATION BY RP-HPLC AND HPTLC METHOD." INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES 05, no. 06 (2018): 5602–10. https://doi.org/10.5281/zenodo.1297120.

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A new simple, accurate, rapid, precise, reproducible and cost effective HPLC and HPTLC method for the quantitative estimation of Rimonabant in bulk and pharmaceutical dosage form. In Method using HPTLC, chromatograms were developed in a mobile phase of 2 mM Sodium dihydrogen orthophosphate (NaH2PO4)-Acetonitrile (38:62) for approximately 25 mins and detected at λmax of 303 nm. Accuracy was found to be in the range of 98.31-102.25 % whereas intraday precision was found to be in the range of 0.03 to 1.60%. Interday precision was found to be 1.36, 0.74 and 0.31 at LQC, MQC and HQC, respect

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Rha, Chan-Su, Yun-Mi Choi, Jong-Chan Kim, and Dae-Ok Kim. "Cost-Effective Simultaneous Separation and Quantification of Phenolics in Green and Processed Tea Using HPLC–UV–ESI Single-Quadrupole MS Detector and Python Script." Separations 8, no. 4 (2021): 45. http://dx.doi.org/10.3390/separations8040045.

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Phenolic composition of green tea (Camellia sinensis) varies according to manufacturing processes that result in deglycosylation of glycosylated phenolics and condensation, epimerization, and degalloylation of flavan-3-ols (catechins). Ambiguous phenolic assignments based on UV absorbance alone can occur when the chromatographic peaks overlapped slightly. We established an improved method using an HPLC–UV coupled with a single-quadrupole MS detector (MS1) that can reject false UV peaks after checking the preceding MS1 peaks. Adjusted UV data coded by the Python algorithm were deployed to compa

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Azadi, Ali, and Shahin Ahmadi. "Simultaneous magnetic dispersive micro solid phase extraction of valsartan and atorvastatin using a CMC-coated Fe3O4 nanocomposite prior to HPLC-UV detection: multivariate optimization." New Journal of Chemistry 43, no. 43 (2019): 16950–59. http://dx.doi.org/10.1039/c9nj03599k.

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In this study, a sensitive, rapid, accurate and practical procedure is established for determination of atorvastatin and valsartan from human biological fluids by dispersive micro solid phase extraction (D-μ-SPE) combined with HPLC-UV detector.

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Sreekanta Nath Dalal, Md Jabir Rashid, and Md Sakil Amin. "Development of a simple RP-HPLC method for the determination of EDTA residue in pharmaceutical clean-in-place (CIP) applications." International Journal of Science and Research Archive 14, no. 3 (2025): 1459–64. https://doi.org/10.30574/ijsra.2025.14.3.0858.

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A reversed-phase method was developed for the determination of EDTA (ethylenediaminetetraacetic acid) in a clean-in-place detergent through HPLC connected with UV or PDA detector. The EDTA signal was well above the detection limit and no interference was observed from water sample or any other swab and rinse diluent. The purpose of this study is to develop a simple, cost-effective reversed-phase HPLC method for the identification and quantification of EDTA in the cleaning solution. This method may contribute to further development and validation of residue detection from cleaning solutions use

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Singh, Neha, Sumit Pannu, Karanvir Singh, Md Jawaid Akhtar, Ankit Anchliya, and Shah Alam Khan. "Application of the Different Analytical Methods for Non-chromophoric Pharmaceutical Compounds." Current Pharmaceutical Analysis 19, no. 8 (2023): 629–51. http://dx.doi.org/10.2174/0115734129255201230925103348.

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Abstract: The physicochemical properties of non-chromophoric compounds that lack a group to absorb UV-visible radiation make them difficult to analyze with a simple detector. Pharmaceutical formulations and their unknown impurities, which show weak or no response with a UV detector, remain undetected and pose a challenge to the analysis of these compounds. Direct measurement of a chromophore complex formed between the compound and the colored ions present in the electrolyte solution with UV detection is one of the validated methods to analyze non-chromophoric compounds. The derivatization with

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Ahmed, Muhammad Arif, Ijaz Khan, Jamshed Hashim, and Syed Ghulam Musharraf. "Sensitive determination of glycerol by derivatization using a HPLC-DAD method in biodiesel samples." Analytical Methods 7, no. 18 (2015): 7805–10. http://dx.doi.org/10.1039/c5ay01761k.

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Transesterification of neutral lipids which gives biodiesel and a UV active by-product glycerol. This article describes the quantification and validation of glycerol based on HPLC using a diode array detector (DAD) in various biodiesel samples through derivatization.

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D., Pavan Kumar a. b. *. Naga Jhansi a. G. Srinivasa Rao b. Kirti Kumar Jain a. "A VALIDATED STABILITY INDICATED RP-HPLC METHOD FOR DUTASTERIDE." Journal of Pharma Research 7, no. 2 (2018): 19–22. https://doi.org/10.5281/zenodo.1182694.

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<em>ABSTRACT</em> <strong><em>A</em></strong><em> Simple, Stability indicating, Isocratic, reverse phase High Performance Liquid Chromatographic (RPLC) related substance method was developed for Dutasteride in API. This method separates the impurities which are co-eluting in the pharmacopeia method. Successful separation of degradation impurities and synthetic impurities was achieved by YMC Triat phenyl column. Chromatographic was carried out on YMC Triat phenyl (</em><em>150 X 4.6 mm, 3.0µm) </em><em>column using 0.01M Potassium Dihydrogen Phosphate, pH to 2.5 with 1mL of trimethylamine

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Nisha, M., Mukund Shankar, Nagasathiya Krishnan, Lilly M. Saleena, Mathur Rajesh, and M. Vairamani. "Direct estimation of ethanol as a negative peak from alcoholic beverages and fermentation broths by reversed phase-HPLC." Analytical Methods 8, no. 23 (2016): 4762–70. http://dx.doi.org/10.1039/c6ay01075j.

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Direct injection of alcoholic samples/broths into a RP-HPLC (with a PDA-UV detector) system to quantify ethanol directly as a negative peak. The kind of peak pair depends on the ethanol concentration as described in the article.

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Paryanti, Sri, Benny Permana, and Sophi Damayanti. "Development and validation of analytical procedure for analysis of polyvinylpyrrolidone in tablets containing metformin hydrochloride and pioglitazone hydrochloride." Pharmacia 72 (May 22, 2025): 1–10. https://doi.org/10.3897/pharmacia.72.e122950.

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Polyvinylpyrrolidone (PVP) is a common excipient in pharmaceutical formulations due to its inert, biocompatible, and multifunctional properties. However, its presence can impact drug release. This study aims to optimize the analysis method for determining PVP concentration in tablets containing a combination of metformin hydrochloride and pioglitazone hydrochloride. The research involved optimizing stationary and mobile phases using HPLC, detector optimization, sample preparation, and stability testing. Validation followed ICH guidelines, covering system suitability, specificity, linearity, ac

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Mutalabisin, Fathiy, Abul Bashar Mohammed Helaluddin, Pinaki Sengupta, Farahidah Mohamed, and Bappaditya Chatterjee. "Quantitation of Pregabalin by HPLC-UV Method using Ninhydrin Derivatization: Development and Validation." Current Pharmaceutical Analysis 17, no. 1 (2020): 165–71. http://dx.doi.org/10.2174/1573412916666191114120213.

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Introduction: A simple and reliable high performance liquid chromatographic method has been developed for the quantitative determination of pregabalin in bulk and dosage form. Pregabalin, a γ amino butyric acid analogue, has negligible sensitivity to UV or fluorescence detection. Hence, it has been derivatized by ninhydrin to form a chromophoric complex that could be quantified by UV detection. Materials and Methods: The concentration of ninhydrin was set to 5 mg/ml and a phosphate buffer solution (pH 7.4) was used as a solvent for the reaction. The resultant complex was separated by HPLC and

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Avula, Bharathi, Yan-Hong Wang, Troy J. Smillie, et al. "Quantitative Determination of Flavonoids by Column High-Performance Liquid Chromatography with Mass Spectrometry and Ultraviolet Absorption Detection in Artemisia afra and Comparative Studies with Various Species of Artemisia Plants." Journal of AOAC INTERNATIONAL 92, no. 2 (2009): 633–44. http://dx.doi.org/10.1093/jaoac/92.2.633.

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Abstract A simple and specific analytical method for the quantitative determination of flavonoids from the aerial parts of the Artemisia afra plant samples was developed. By column high-performance liquid chromatography (HPLC) with UV absorption and mass spectrometry (MS) detection, separation was achieved on a reversed-phase octadecylsilyl (C18) column with water, methanol, and acetonitrile, all containing 0.1 acetic acid, as the mobile phase. These methods were used to analyze various species of Artemisia plant samples. The wavelength used for quantification of flavonoids with the diode arra

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Yantih, Novi, Yahdiana Harahap, Wahono Sumaryono, Rianto Setiabudy, and Lestari Rahayu. "Separation Selectivity of Isoniazid and Asetilisoniazid in Human Plasma In-vitro by High Performance Chromatography." JURNAL ILMU KEFARMASIAN INDONESIA 17, no. 2 (2019): 238. http://dx.doi.org/10.35814/jifi.v17i2.717.

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Bioanalysis method is needed to pharmacokinetic study of INH as antituberculosis. The main problem is INH structure is similar to that of acetyl isoniazid (AcINH) as its metabolite. Therefore, a selective separation method is needed to separate the INH from its metabolite and matrix. The aim of this study was to test the selectivity of separation method of INH and AcINH in human plasma in-vitro by high performance liquid chromatography (HPLC). The HPLC system used a reversed phase with UV detector and hexane sulphonate as an ion counter. The optimum conditions was obtained by using C18 as stat

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Shaik, Javed S., and Nutan N. Rao. "SIMULTANEOUS ESTIMATION AND FORCED DEGRADATION STUDIES OF AMILORIDE HYDROCHLORIDE AND FUROSEMIDE IN A PHARMACEUTICAL DOSAGE FORM USING REVERSE-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY METHOD." Asian Journal of Pharmaceutical and Clinical Research 11, no. 7 (2018): 215. http://dx.doi.org/10.22159/ajpcr.2018.v11i7.25783.

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Objective: The present study describes the stability indicating reverse-phase high-performance liquid chromatography (RP-HPLC) method for simultaneous estimation of amiloride hydrochloride and furosemide in pharmaceutical dosage forms.Methods: The proposed RP-HPLC method was developed using Shimadzu LC-2030 HPLC system equipped with UV detector, and chromatographic separation was carried on Shim-pack C18 (250 mm×4.6 mm, 5 μ) column at a flow rate of 1 ml/min and the runtime was 4min. The mobile phase consisted of water and acetonitrile in the ratio of 35:65, and elements were scanned using a U

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Radi Karabat, Raad, Ali Jasim Hammood, Wad Abdulhusein Abdulrasool, and Sabah Salam Hussein. "High-Performance Liquid Chromatography (HPLC): Primary Mechanism and Popular Applications." Al-Nahrain Journal of Science 26, no. 4 (2023): 1–12. http://dx.doi.org/10.22401/anjs.26.4.01.

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The concept chromatography can be used to refer to a huge variety of distinct analytical procedures. Each of these procedures utilizes a mobile phase and an immobile phase as a basic component, yet they all belong under the chromatography umbrella concept. The subsequent component separation that is carried out is based on the disparity in concentration ratio that can be detected between the two phases. This disparity acts as the basis for the subsequent component separation that is carried out. Due to the limits of current technology, there is presently no detector that is universally applica

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Mistry, Vaishali, and Rohan Mishra. "SIMULTANEOUS ESTIMATION, VALIDATION, AND FORCED DEGRADATION STUDIES OF BETAHISTINE DIHYDROCHLORIDE AND DOMPERIDONE IN A PHARMACEUTICAL DOSAGE FORM USING RP-HPLC METHOD." Asian Journal of Pharmaceutical and Clinical Research 11, no. 10 (2018): 125. http://dx.doi.org/10.22159/ajpcr.2018.v11i10.26132.

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Objective: This study describes the stability-indicating reverse-phase high-performance liquid chromatography (RP-HPLC) method for simultaneous estimation of betahistine dihydrochloride and domperidone in pharmaceutical dosage forms.Methods: The proposed RP-HPLC method was developed using Shimadzu Prominence-i LC-2030 HPLC system equipped with UV detector and chromatographic operation was carried on Shim-pack C18 (250 mm×4.6 mm, 5 μ) column at a flow rate of 1 ml/min and the run time was 10 min. The mobile phase consisted of methanol and water in the ratio of 80:20% v/v and eluents were scanne

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Rudakov, Yaroslav O., Vladimir F. Selemenev, Ludmila V. Rudakova, and Oleg B. Rudakov. "Chromatographic approaches to food quality control by chemical compo-sition." Сорбционные и хроматографические процессы 24, no. 2 (2024): 197–208. http://dx.doi.org/10.17308/sorpchrom.2024.24/12125.

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The article provides an overview of chromatographic methods, which, when used comprehensively, ensure food quality control and safety. It considers gas chromatography methods that allow analysing volatile components in products using a flame ionization detector, an electron capture detector, and mass-selective detectors (GC-FID, GC-ECD, GC-MS, and GC-MS/MS). The article provides a list of analytes that can be detected by high performance liquid chromatography in combination with refractometric, spectrometric, and mass selective detectors (HPLC-RMD, HPLC-UV, HPLC-SPD, HPLC-MS, and HPLC-MS/MS).

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Hanh, Luong Thi My, Nguyen Thi Minh Diep, Pham Thi Ngoc Mai, and Nguyen Xuan Truong. "HIGH PERFORMANCE LIQUID CHROMATOGRAPHY ASSAY FOR DETERMINATION OF MOXIFLOXACIN IN HUMAN PLASMA." Vietnam Journal of Science and Technology 57, no. 3 (2019): 336. http://dx.doi.org/10.15625/2525-2518/57/3/13362.

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A simple reversed phase HPLC method with UV detection has been successfully developed and validated for determination of moxifloxacin in human plasma. The sample pretreatment involves only single-step protein precipitation with tricloroacetic acid. Moxifloxacin was measured in plasma using a validated HPLC method with UV detector at 295 nm, C18 column (25cm×4.5mm, 5µm), a mixture of phosphate buffer pH 4.0 and acetonitrile (70:30, v/v) as mobile phase at a flow rate of 0.8 ml/min. Retention time of moxifloxacin was found to be 7.4 min. The mean recovery for the drug was obtained 97.30%. The ca

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Iqbal, Masrori, Dedy Suhendra, and Erin Ryantin Gunawan. "The separation of alkyldiethanolamide based on Kernel oil of Calophyllum inophillum fruit using High-Performance Liquid Chromatography." Acta Chimica Asiana 5, no. 1 (2022): 153–57. http://dx.doi.org/10.29303/aca.v5i1.78.

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This study aimed to separate the synthesized alkyldiethanolamide from kernel oil Calophyllum inophyllum (Local name: Nyamplung) using High-Performance Liquid Chromatography (HPLC). HPLC column utilized was SGE ODS-2 reverse phase and UV detector 213 nm. The variables to obtain the optimum conditions for separating alkyldiethanolamide were mobile phase and flow rate. The mobile phase composition and the optimum separation flow rate obtained acetonitrile: water (90:10) and 1.5 mL/minute, respectively. The percentage compositions of amide fatty acids that had been successfully synthesized based o

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Pogosyan, Ruben Ashotovich, Olga Vladimirovna Nesterova, Dmitry Olegovich Bokov, and Irina Alexandrovna Samylina. "QUANTITATIVE DETERMINATION OF PHENOL CARBOXYLIC ACIDS IN POMEGRANATE FRUIT PULP BY THE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH ULTRAVIOLET DETECTION." Asian Journal of Pharmaceutical and Clinical Research 12, no. 1 (2019): 548. http://dx.doi.org/10.22159/ajpcr.2018.v12i1.30589.

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Objective: Pomegranate (Punica granatum L.) is a broadly used plant possessing a wide range of medicinal properties. In this research, we have mainly focused on the investigation of phenolic compounds of pomegranate fruit pulp (PFP).Methods: Fresh fruits of “Çəhrayı Gülöyşə,” “Kizil-anor,” and pomegranate varietal mixture were used as samples. High-performance liquid chromatography-ultraviolet (HPLC-UV) analysis of phenol carboxylic acids was performed with metal column Kromasil® C18 (4.6×250 mm, particle size 5 μm) and the acetonitrile-water-concentrated acid phosphoric system (400:600:5) und

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Pogosyan, Ruben Ashotovich, Olga Vladimirovna Nesterova, Dmitry Olegovich Bokov, and Irina Alexandrovna Samylina. "QUANTITATIVE DETERMINATION OF PHENOL CARBOXYLIC ACIDS IN POMEGRANATE FRUIT PULP BY THE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH ULTRAVIOLET DETECTION." Asian Journal of Pharmaceutical and Clinical Research 12, no. 1 (2019): 548. http://dx.doi.org/10.22159/ajpcr.2019.v12i1.30589.

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Objective: Pomegranate (Punica granatum L.) is a broadly used plant possessing a wide range of medicinal properties. In this research, we have mainly focused on the investigation of phenolic compounds of pomegranate fruit pulp (PFP).Methods: Fresh fruits of “Çəhrayı Gülöyşə,” “Kizil-anor,” and pomegranate varietal mixture were used as samples. High-performance liquid chromatography-ultraviolet (HPLC-UV) analysis of phenol carboxylic acids was performed with metal column Kromasil® C18 (4.6×250 mm, particle size 5 μm) and the acetonitrile-water-concentrated acid phosphoric system (400:600:5) und

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Smolinska, Mariia, Roman Ostapiv, Mariia Yurkevych, et al. "Determination of Benzalkonium Chloride in a Disinfectant by UV Spectrophotometry and Gas and High-Performance Liquid Chromatography: Validation, Comparison of Characteristics, and Economic Feasibility." International Journal of Analytical Chemistry 2022 (September 19, 2022): 1–8. http://dx.doi.org/10.1155/2022/2932634.

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Simple, fast, and validated UV-spectrophotometric, HPLC, and GC methods for the analysis of benzalkonium chloride in a disinfectant were developed. UV-spectrophotometric determination was based on measuring the amount of light absorption of aqueous solutions of benzalkonium chloride at 268 nm. HPLC determination was achieved with a 150 mm × 4.6 mm, 5.0 μm C18 column. The mobile phase consisted of a 0.01% water solution of triethylamine (with pH 2.5) and acetonitrile in the ratio of 40 : 60 v/v. The column temperature was kept at 30°C, and the injection volume was 10 μL. The flow rate was 1.0 m

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Kaushik, Patel. "Analytical method for simultaneous estimation of voglibose and mitiglinide calcium hydrate by validated RP-HPLC method." Journal of medical pharmaceutical and allied sciences 11, no. 4 (2022): 5066–71. http://dx.doi.org/10.55522/jmpas.v11i4.2646.

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A combination of Voglibose and Mitiglinide calcium hydrate is used in Type- 2 diabetes. The method was based on pre-column derivatization of Voglibose with 9-Fluorenylmethyloxycarbonyl chloride due to lack of chromophoric group it cannot directly estimated by UV detector in Reverse Phase High Performance Chromatography (RP-HPLC) method. The chromatographic separation was achieved using phosphate buffer (pH 4.0) and methanol (30:70) as mobile phase. Detection was carried out at 233 nm using UV detector. The retention time of Voglibose and Mitiglinide calcium hydrate were found to be 5.233 min a

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Darade, Ramdas, and Sanjay Pekamwar. "Quality by design driven RP-HPLC method optimization for analysis of levothyroxine and liothyronine in bulk and tablet dosage form." Journal of Applied Pharmaceutical Research 13, no. 2 (2025): 139–48. https://doi.org/10.69857/joapr.v13i2.867.

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Background: Levothyroxine and Liothyronine are widely used in thyroid hormone replacement therapies. Simultaneously quantifying Levothyroxine and Liothyronine is important for managing thyroid hormone deficiency. Aim: This study aims to develop and validate an accurate and robust RP-HPLC method for simultaneously quantifying Levothyroxine and Liothyronine by utilizing Quality by Design (QbD). Methodology: Reversed phase chromatography was performed using a High Performance Liquid Chromatographic System (Agilent Technologies Ltd, 1100 series) equipped with a UV detector. The column used was Agi

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Bui, Duy Anh, Benjamin Bomastyk, and Peter C. Hauser. "Absorbance detector based on a deep UV light emitting diode for narrow-column HPLC." Journal of Separation Science 36, no. 19 (2013): 3152–57. http://dx.doi.org/10.1002/jssc.201300598.

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Zhang, Xingping, Jiujun Wang, Qinghua Wu, Li Li, Yun Wang, and Hualin Yang. "Determination of Kanamycin by High Performance Liquid Chromatography." Molecules 24, no. 10 (2019): 1902. http://dx.doi.org/10.3390/molecules24101902.

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Kanamycin is an aminoglycoside antibiotic widely used in treating animal diseases caused by Gram-negative and Gram-positive infections. Kanamycin has a relatively narrow therapeutic index, and can accumulate in the human body through the food chain. The abuse of kanamycin can have serious side-effects. Therefore, it was necessary to develop a sensitive and selective analysis method to detect kanamycin residue in food to ensure public health. There are many analytical methods to determine kanamycin concentration, among which high performance liquid chromatography (HPLC) is a common and practica

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Debebe, Ayalew, Shibru Temesgen, Mesfin Redi-Abshiro, Bhagwan Singh Chandravanshi, and Estifanos Ele. "Improvement in Analytical Methods for Determination of Sugars in Fermented Alcoholic Beverages." Journal of Analytical Methods in Chemistry 2018 (October 8, 2018): 1–10. http://dx.doi.org/10.1155/2018/4010298.

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The main objective of this study was to improve the performance of analytical methods for the determination of sugars in fermented alcoholic beverages based on mid-infrared-partial least squares (MIR-PLS), high-performance liquid chromatography with the ultraviolet detector (HPLC-UV), high-performance liquid chromatography with the refractive index detector (HPLC-RI), and sulfuric acid methods. The MIR-PLS method was found to give good prediction of individual sugars: glucose, fructose, sucrose, and maltose in the alcoholic beverages with less than 4% error. The HPLC-UV method can be used for

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Abdulra’uf, L. B., F. A. Adeyemo, F. B. Atanda, and R. Lawal. "Detection Of Pendimethalin and Cypermetrin Residues in Locally Produced Tomato Using QuEChERS-HPLC Analysis." Nigerian Journal of Basic and Applied Sciences 27, no. 1 (2020): 34–40. http://dx.doi.org/10.4314/njbas.v27i1.5.

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This study investigated the levels of pendimethalin and cypermetrin residues in tomato sold in Malete market, Moro Local Government Area of Kwara State. Tomatoes were randomly collected from five different vendors in Malete market and analysis was performed using the QuEChERS (Quick, Easy Cheap, Effective, Rugged and Safe) method followed by chromatographic analysis using high performance liquid chromatography (HPLC) coupled to ultra-violet (UV) detector. Method validation of the study showed a linearity of the analytes which ranges from 5 – 500 μg/kg, with correlation coefficients greater tha

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Amarowicz, R., and S. Weidner. "Content of phenolic acids in rye caryopses determined using DAD-HPLC method." Czech Journal of Food Sciences 19, No. 6 (2013): 201–5. http://dx.doi.org/10.17221/6608-cjfs.

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Phenolic compounds were extracted from rye caryopses with 80% (v/v) methanol. Phenolic acids were determined as free compounds and those liberated from soluble esters and glycosides. The analyses were performed using a Waters HPLC system equipped with a diode array detector (DAD). The following free phenolic acids were found: p-coumaric, ferulic and sinapic; the phenolic acids liberated from soluble esters were as follows: vanillic, caffeic, p-coumaric, ferulic and sinapic; and those liberated from soluble glycosides were the following: vanillic, p-coumaric, ferulic and sinapic. In rye caryops

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Cano García, Arely, Yisa María Ochoa Fuentes, Augusto Gil Ceballos Ceballos, and Ernesto Cerna Chávez. "Residuos de glifosato en cereales de desayuno de origen internacional y nacional comercializados en Saltillo, Coahuila." Horizonte Sanitario 22, no. 2 (2023): 383–91. http://dx.doi.org/10.19136/hs.a22n2.5489.

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Objective: To detect and quantify glyphosate residues in breakfast cereals marketed in the city of Saltillo, Coahuila. Materials and Methods: Two samples of 12 different cereal brands were analyzed. A Soxhlet apparatus was used for pesticide extraction and quantification was performed by High Performance Liquid Chromatography (HPLC) using an Agillent model 1100 Series equipment coupled to a UV-Vis detector. Results: Glyphosate was detected in 100% of the samples analyzed, with concentrations ranging from 170 to 2 400 mg/kg; the samples with the highest concentrations were those of internationa

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Gujral, Rajinder Singh, and Sk Manirul Haque. "Development and Validation of a New HPLC Method for the Determination of Gabapentin." International Journal of Biomedical Science 5, no. 1 (2009): 63–69. http://dx.doi.org/10.59566/ijbs.2009.5063.

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A simple HPLC method was developed and validated for quantitation of gabapentin in pure form. The HPLC separation was achieved on a C18 5 μm Waters column (150 mm × 4.6 mm) using a mobile phase of methanol - potassium dihydrogen orthophosphate solution (20:80, v/v) containing 10% NaOH to adjust pH6.2 at a flow rate of 1.0 ml/min. The UV detector was operated at 275 nm. The method was validated for specificity, linearity, precision, accuracy, robustness and limit of quantitation. The degree of linearity of the calibration curves, the percent recoveries, limit of detection and quantitation for t

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