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Dissertations / Theses on the topic 'HPLC with fluorescence detection'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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1

Andersson, Anja. "Method development for the determination of thiols using HPLC with fluorescence detection." Thesis, Umeå universitet, Kemiska institutionen, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-59827.

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2

Smith, Eileen Mary. "The determination of alkylphenols in natural waters by SPE-HPLC-fluorescence detection." Thesis, Nottingham Trent University, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.366072.

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3

Liao, Jing-Piin. "Investigation of Copper-Natural Ligand Complexes by RP-HPLC Photodiode Array UV-VIS and Fluorescence Detection." Thesis, University of North Texas, 1992. https://digital.library.unt.edu/ark:/67531/metadc332502/.

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In this study, reversed phase HPLC with dual UV photodiode (PDA) and fluorescence (FL) detection were used to investigate copper complexes with fulvic, caffeic, vanillic, salicylic, and adipic acids. Application of the RE method provided valuable information on the retention behavior and spectral characteristics of FA and model compounds. Even though the method was only applicable to VA, the use of the PDA detector allowed the UV-V is scanning of the separated peaks. This allowed the comparison between the UV-Vis spectra of uncomplexed species. The overall results provide an experimental frame

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4

Dotlich, Erin Michele. "ALKYLAMMONIUM FORMATE IONIC LIQUIDS AS SOLVENTS FOR FLUORESCENCE AND LIQUID CHROMATOGRAPHY METHODS." Miami University / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=miami1208983119.

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5

Dussex, Dusten T. "The Utilization of Fluorescent Products to Detect the Inhibition of Acetylcholinesterase by Carbamates and Organophosphate Pesticides: The Groundwork For a New Assay." University of Cincinnati / OhioLINK, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1470743777.

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6

Lea, Krista La Moen. "TALL FESCUE ERGOVALINE CONCENTRATION BASED ON SAMPLE HANDLING AND STORAGE METHOD." UKnowledge, 2014. http://uknowledge.uky.edu/pss_etds/35.

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Ergovaline is produced by the endophyte Neotyphodium coenophialum (Morgan-Jones and Gams) in tall fescue (Schedonorus arundinacea (Schreb.) Dumort. = Festuca arundinacea Schreb.) and is blamed for a multitude of costly livestock disorders. Testing of pastures is common in both research and on farm situations. Since ergovaline is known to be unstable and affected by many variables, the objective of this study was to determine the effect of sample handling and storage on the stability of this compound. Homogeneous milled tall fescue sub-samples were analyzed for ergovaline concentration using HP

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7

Kadkhodaei-Elyaderani, Manizheh. "HPLC detection of haemoglobinopaties." Thesis, University of Salford, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.308175.

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8

Thomas, H. D. "Chemiluminescence, a detection method for HPLC." Thesis, University of East Anglia, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.383774.

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9

Herzog, Dylan Benjamin. "Rapid fluorescence amplified biofilm detection." Thesis, King's College London (University of London), 2017. https://kclpure.kcl.ac.uk/portal/en/theses/rapid-fluorescence-amplified-biofilm-detection(a6f7d42b-941b-441b-9be1-f591978fb883).html.

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Bacterial biofilms are the cause of many diseases linked to persistent bacterial infection. When remaining in the root canal space following endodontic treatment, bacteria can lead to a secondary infection, resulting in treatment failure and the subsequent need for re-intervention. Currently, there are no methods in use to detect bacterial presence within the root canal space in a fast and reliable manner. Such methods would be especially beneficial in determining the end point of root canal treatments. Here, we make use of molecular fluorescent dyes and fluorescent microscopy/spectroscopy to

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10

Guo, Yixing. "Fluorescence Detection of Biological Thiols." PDXScholar, 2012. https://pdxscholar.library.pdx.edu/open_access_etds/586.

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Glutathione (GSH) is an important biological thiol, it performs significant biological functions such as serving an antioxidant which protect cells from oxidative stress by trapping free radicals which damage DNA and RNA. It is known that abnormal plasma levels of GSH have been linked to various human diseases. Therefore, the rapid, sensitive and highly selective detection of GSH is of great importance for investigating its functions in diseases diagnosis. Interestingly, we found in cetyl trimethylammonium bromide (CTAB) medium, the resorufin-based probe shows an extremely fast, highly selecti

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11

Abbott, R. W. "HPLC of drugs of abuse with chemiluminescence detection." Thesis, University of Hull, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.384671.

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12

Sun, Kailiang. "Fluorescence based optical sensor for protein detection." Birmingham, Ala. : University of Alabama at Birmingham, 2008. https://www.mhsl.uab.edu/dt/2010r/ksun.pdf.

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13

Bagheri, Habib. "Vapour-phase fluorescence detection in gas chromatography." Thesis, University of East Anglia, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.280036.

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14

Marr, J. G. D. "Computer-aided detection systems for HPLC : Development, assessment and application of digital techniques for peak purity validation in HPLC utilising photodiode array detection." Thesis, University of Bradford, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.384261.

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15

Wintergerst, Mieke. "Detection of inositol phosphates with HPLC-ICP-AES : Method development." Thesis, Uppsala universitet, Analytisk kemi, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-202003.

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Inositol phosphates (IPs) represent a major part of the organic phosphorus found in the environment, which makes their identification and quantification very important. The goal of this project was to explore the possibility of quantification of IPs with inductively coupled plasma - atomic emission spectrometry (ICP - AES). This paper deals with the creation of an in-house IP standard and the considerations for the successful linking of high performance liquid chromatography (HPLC) with ICP - AES. Experiments with different nebulizers, mobile phases, standard solutions and model substance were

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16

Harrison, Joanne Clare. "Immunological and HPLC detection of aflatoxin Bâ†1-DNA adducts." Thesis, University of York, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.304060.

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17

Liu, Gaoyuan. "Chiral and cyclodextrin-based phases in HPLC with polarimetric detection." Thesis, University of York, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.297137.

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18

Machado, Saulo de Tarso Zacarias. "Validação de método para detectar resíduos de ivermectina em leite bovino." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/10/10134/tde-12082015-165003/.

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Níveis não aceitáveis de resíduos de ivermectina (IVM), uma droga anti-parasitária amplamente utilizada no Brasil para controle de endectoparasitas, pode estar presente no leite para consumo humano se administrada incorretamente. O nível máximo de resíduos no leite para este composto é de 10 ng mL-1 e sua presença tem sido comprovada em pesquisas realizadas por orgãos reguladores. Com o conhecimento deste problema, um método para a detecção de ivermectina em amostras de leite foi desenvolvido utilizando a extração líquido-líquido com base em acetonitrila e hexano, seguida por derivatização com

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19

Lee, Kevin Shao-Kwan. "Large core polymer optical backplanes for fluorescence detection." Thesis, Massachusetts Institute of Technology, 2006. http://hdl.handle.net/1721.1/37944.

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Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2006.<br>Includes bibliographical references (p. 203-205).<br>Fluorescence based sensors are used for determining environmental parameters such as dissolved oxygen or pH in biological systems without disturbing a biological system's equilibrium. Recently, there has been a drive to provide biological analysis tools in a compact form, resulting in arrays of miniature devices which can perform multiple functions in parallel such as bacteria cultures or DNA analysis. As these new types of ch

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20

Hindocha, Ravinder K. "Liquid-liquid extraction with long wavelength fluorescence detection." Thesis, Loughborough University, 1994. https://dspace.lboro.ac.uk/2134/31792.

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Long wavelength fluorophores, methylene blue, Rhodamine 800, 3,3'diethyloxacarbocyanine iodide (DODC) and 1, 1',3,3,3' ,3' -hexamethylindotricarbocyanine perchlorate (HIDTCP) were investigated as probes to quantify drugs after liquid-liquid extraction. Fluorescence measurements in the long wavelength region (550-1000nm) are a recent development in photoluminescence spectroscopy and offer many advantages compared with conventional measurements which are made in the ultraviolet and visible spectral regions. These include, reduced background fluorescence in the presence of biological materials; r

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21

Thornton, Alexander R. "Post-column photolysis HPLC detector for N-nitroso compounds." Thesis, Heriot-Watt University, 1988. http://hdl.handle.net/10399/936.

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22

Lien, Wan-Fu. "Separation of Transition Metal Ions by HPLC, Using UV-VIS Detection." Thesis, North Texas State University, 1987. https://digital.library.unt.edu/ark:/67531/metadc501145/.

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HPLC has been used and can quickly determine several ions simultaneously. The method of determination described for transition metals [Cr(III), Fe(III), Ni(II), Co(II), Cu(II), Zn(II), Cd(II), Mn(II)] and [Ca(II), Pb(II)] using HPLC with UV-VIS detection is better than the PAR complexation method commonly used. The effects of both eluent pH and detector wavelength were investigated. Results from using different pHs and wavelengths, optional analytical conditions for the separation of [Ni(II), Co(II), Cu(II)], [Cr(III), Fe(III), Ca(II), Ni(II), Cu(II)], and [Ca(II), Zn(II), Pb(II)] in one injec

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23

Alexander, Andrew Lafayette. "Evaluation of illumination wavelengths for fluorescence detection of atherosclerosis." Diss., The University of Arizona, 1994. http://hdl.handle.net/10150/186607.

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The fluorescence-emission spectra of normal arterial tissues and atherosclerotic plaques are distinct. The fluorescence spectra of arterial tissues are also dependent on the illumination wavelength. Which illumination wavelengths are best for identifying arterial tissues? To answer this question, a system for measuring fluorescence spectra at different illumination wavelengths was constructed and the fluorescence spectra of in-vitro aorta specimens were acquired with several illumination wavelengths between 270 nm and 500 nm. The Mahalanobis distance, a statistical measure of separability betw

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24

Kownarumit, Supaporn. "Multiplex screening using enzyme inhibition, fluorescence detection and chemometrics." Thesis, Loughborough University, 2006. https://dspace.lboro.ac.uk/2134/12308.

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Fluorescence enzyme inhibition assays have been established for a number of years as valuable methods of analysis in clinical chemistry and other fields. Those in common use are normally single analyte assays. However, in many cases (e.g. drug screening) dual or multiplex assays would be much more valuable, with the advantages of increased information content, saving in time and costs, and the elimination of some sources of sampling variance. This project has investigated single and dual screening assays of enzyme inhibitors, namely 3-nitrophenylboronic acid (3-NPBA), phenylethyl /3-0- thiogal

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25

Wakefield, Clare Helen. "The remote detection of uranium leaks using fluorescence characteristics." Thesis, University of Salford, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.313871.

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26

Wang, Thomas D. (Thomas Duen-Shyr). "Fluorescence endoscopic imaging system for detection of colonic adenomas." Thesis, Massachusetts Institute of Technology, 1996. http://hdl.handle.net/1721.1/8972.

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Thesis (Ph.D.)--Massachusetts Institute of Technology, Whitaker College of Health Sciences and Technology, 1996.<br>Includes bibliographical references (p. [137]-144).<br>Background/Aims: Autofluorescence spectra have been collected from colonic mucosa with optical fiber contact probes. This technique was found to be sensitive to the biochemical and microarchitectural differences between normal and pre-malignant tissue. It is desired to extend this method to wide area surveillance using endoscopically-collected fluorescence images. Methods: An analytic model was developed to determine the numb

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27

Kwon, Sun Kuk. "Dynamic fluorescence imaging with molecular agents for cancer detection." [College Station, Tex. : Texas A&M University, 2006. http://hdl.handle.net/1969.1/ETD-TAMU-1064.

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28

Maretti, Luca. "Fluorescence detection of carbon-centered radicals in different systems." Thesis, University of Ottawa (Canada), 2002. http://hdl.handle.net/10393/6190.

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The work presented in this thesis is primarily focused on the development of pre-fluorescent probes for carbon-centered radicals. Coumarin-TEMPO and quinoline-TEMPO adducts have been prepared by tethering a free radical nitroxide group such as 2,2,6,6-tetramethyl-1-piperidinyloxy (TEMPO) to coumarin and quinoline fluorophores through an ester bond. Some organic derivatives of the quinoline-TEMPO adduct are also presented. Intramolecular quenching of fluorescence due to a spin-exchange mechanism between the singlet excited state of the fluorophore and the doublet ground state of the nitroxide a

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29

Nolden, Raphael. "Studies of DPA Fluorescence Enhancement." Thesis, University of Canterbury. Physics and Astronomy, 2007. http://hdl.handle.net/10092/4414.

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The processes involved in the enhancement of the fluorescence profile of dipicolinic acid (DPA) were measured and analysed, with particular emphasis on their potential application to the rapid identification of suspicious powders. The research was conducted in contribution to the anthrax detector currently under development at this department. Using the enhancement of fluorescence as a method of determining whether a sample contains spores shows great potential because DPA is not found in most powders that do not contain spores. Thus, its detection is a good indication of the presence of spore

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30

Buranaphalin, Sawanya. "Pharmaceutical analysis of polyamines and aminoglycosides." Thesis, University of Bath, 2009. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.500693.

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Methods for polyamine derivatization with a panel of extrinsic fluorophores followed by HPLC with fluorescence and UV absorption detection have been developed. Four fluorophores were examined using polyamines and aminoglycosides. o-Phthalaldehyde (OPA) and fluorescamine are selective fluorophores that only react with primary amines; 9- fluorenylmethyl chloroformate (FMOC Cl) and dansyl chloride react with both primary and secondary amines. Reaction and HPLC conditions were optimized with each of the above fluorophores using a series of model mono- and diamines and then applied to natural and s

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31

Juaristi, Ane Iruretagoiena. "HPLC-FL for determination of hormones in bivalves." Master's thesis, Universidade de Aveiro, 2014. http://hdl.handle.net/10773/14301.

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Mestrado em Química - Química Analítica e Qualidade<br>The main objective of this work was the application of an isocratic high performance liquid chromatography (HPLC) method coupled with a fluorescence detector to find the best conditions for the separation and quantification of 17β-estradiol (E2) and 17α-ethinyl estradiol (EE2) hormones in bivalve samples. A reversed phase column (Kromasil 100 C18, 5μm, 15 x 0.21cm), was employed with a fluorescence detector operating at excitation and emission wavelengths of 280nm and 305nm, respectively. A calibration curve was obtained with a good correl

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32

Araujo, Juliana Coutinho de. "\"Estudo da eficiência do tratamento de efluentes domésticos da cidade de Araraquara-SP na remoção de hormônios sexuais\"." Universidade de São Paulo, 2006. http://www.teses.usp.br/teses/disponiveis/75/75132/tde-17012007-144901/.

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Nos últimos anos, a pesquisa ambiental tem se defrontado com a questão dos chamados disruptores endócrinos (EDCs). A estes compostos, tais como: produtos farmacêuticos, hormônios naturais e sintéticos, pesticidas, substâncias tensoativas, polímeros de baixa massa molecular e diversos outros contaminantes orgânicos presentes em efluentes municipais e industriais, atribui-se à capacidade de alterar o funcionamento do sistema endócrino. Estrogênios e progestogênios, naturais ou sintéticos, são excretados pela urina de mamíferos, e uma pequena porção nas fezes, e via efluentes de estações de trata

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33

Ziegler, Stephan Johannes. "Selective and sensitive detection methods for HPLC analysis of selected medicinal plants /." [S.l.] : [s.n.], 1988. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=8721.

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34

McCarthy, Patrick Kieran. "HPLC and immunochemical detection of gliadin impurities in wheat and wheat products." Thesis, Nottingham Trent University, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.253810.

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35

Owino, Erisa. "Computer-aided spectrophotometric detection in HPLC for the examination of drug stability." Thesis, University of Bradford, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.305662.

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36

Ji, Honghao. "Integrated CMOS optical sensors for fluorescence detection and contact imaging." College Park, Md. : University of Maryland, 2006. http://hdl.handle.net/1903/3890.

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Thesis (Ph. D.) -- University of Maryland, College Park, 2006.<br>Thesis research directed by: Electrical Engineering. Title from t.p. of PDF. Includes bibliographical references. Published by UMI Dissertation Services, Ann Arbor, Mich. Also available in paper.

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37

Halverson, Peter Georges. "Detection of high-energy cosmic ray showers by atmospheric fluorescence." Diss., The University of Arizona, 1989. http://hdl.handle.net/10150/184779.

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A novel detector for ultra-high energy cosmic rays, and its prototype are discussed. It detects events with primary energy greater than 100 PeV. (1 PeV = 1000 TeV; 1EeV = 1000 PeV.) The detector operates by sensing the near-ultraviolet scintillation light of ionized nitrogen molecules created by the passage of ionizing particles in extensive air showers. (The concept is loosely based on the highly successful Fly's Eye detector situated at Dugway, Utah.) Typical events should consist of 1 to 100 EeV primary energy showers, with near-vertical cores, passing through the detector's field-of-view a

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38

Hill, Elisabeth Katherine. "Development of confocal fluorescence detection for single molecules in solution." Thesis, Imperial College London, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.392100.

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39

Khokhar, Muhammad Y. "Flow injection immunoassays using solid phase immunoreactors and fluorescence detection." Thesis, Loughborough University, 1993. https://dspace.lboro.ac.uk/2134/12384.

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The use of flow injection analysis with fluorescence detection was evaluated using the host-guest phenomenon between the cyclodexnins and DL-Iysine and Lserine. Auorescence enhancement, kinetic and equilibrium studies were recorded and the effect of pH and time on fluorescence were also observed. Rhodamine isothiocyanate was conjugated to insulin. Insulin and dye were mixed in different ratios, and the dye : insulin ratio was detennined for each conjugate. These conjugates were checked for immunoreactivity. Insulin-biotin and antibody-iminobiotin conjugates were also prepared. Insulin : biotin

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40

Lundqvist, Anders. "Nanoscale biochemical analysis using capillary electrophoresis and confocal fluorescence detection /." Göteborg : Göteborg university, 2002. http://catalogue.bnf.fr/ark:/12148/cb40115032p.

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41

Li, Li. "Detection of Proteins by Two-Photon Excitation of Native Fluorescence." Diss., CLICK HERE for online access, 2006. http://contentdm.lib.byu.edu/ETD/image/etd1548.pdf.

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42

Staley, Lindsey M. "Detection of Bacteriophage Infection Using Absorbance, Bioluminescence, and Fluorescence Tests." University of Dayton / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1304008676.

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43

Xue, Yuanyuan. "System Model of an Ultra-LowPower Fluorescence Detection Micro-Device." Thesis, Linköpings universitet, Elektroniska Kretsar och System, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-172133.

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This thesis aims to explore the power consumption limits of Complementary Metal-Oxide-Semiconductor (CMOS) fluorescent biosensors that meet the demand, a Matlab-based calculator is designed, in which users can input required system parameters (such as LED power and noise) to observe the minimum power consumption of each basic component and the constraints of the transimpedance amplifier: cut-off frequency, signal- to-noise ratio, dynamic range, etc. Thereby to provide a reference range for the next level designs of each component in the detection system. In our top-level model, only essential

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44

McCracken, Katherine E., Trinny Tat, Veronica Paz, and Jeong-Yeol Yoon. "Smartphone-based fluorescence detection of bisphenol A from water samples." ROYAL SOC CHEMISTRY, 2017. http://hdl.handle.net/10150/623041.

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Bisphenol A (BPA), an emerging environmental contaminant and endocrine disrupting compound, has been observed globally in surface water and waste leachates at concentrations that are hazardous to aquatic life and potentially to humans. Limitations in field monitoring on account of the extensive laboratory infrastructure required for standard BPA detection warrants investigation into portable or handheld sensing platforms. In this work, we evaluated a standalone smartphone-based fluorescence sensing method for identifying BPA from water samples. Toward this goal, we demonstrated the novel appli

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45

Tian, Yunxian. "Anti-Stokes fluorescence spectroscopy and imaging for cutaneous porphyrin detection." Thesis, University of British Columbia, 2015. http://hdl.handle.net/2429/52082.

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Porphyrins produced by Propionibacterium acnes represent the principal fluorophore associated with acne, and appear as orange-red luminescence under the Wood’s lamp. Assessment of acne based on Wood’s lamp (UV) or visible (VIS) light illumination is limited by photon penetration depth and has limited sensitivity for earlier stage lesions. Inducing fluorescence with near infrared (NIR) excitation may provide an alternative way to assess porphyrin-related skin disorders. The objectives of this thesis are (1) to design and develop an optical instrument that perform anti-Stokes fluorescence spectr

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46

Parmar, Dikesh, and n/a. "Detection of dentine tubule infection." University of Otago. School of Dentistry, 2007. http://adt.otago.ac.nz./public/adt-NZDU20071221.115951.

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Bacteria are implicated in endodontic infections. They not only infect the root canal lumen but also invade the dentinal tubules where they may remain untouched by contemporary chemomechanical preparation during root canal therapy. The contentious issue is whether the bacteria within these tubules contribute to secondary infections. Many studies have shown that clinicians fail to completely eradicate them during root canal therapy. At present there are no techniques available to detect the effectiveness of the current chemomechanical treatment regime within dentinal tubules. It is difficult

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47

Camp, Sharon Kay Ritchie. "The HPLC separation and electrochemical detection of azo dyes and their degradation products." Diss., Georgia Institute of Technology, 1989. http://hdl.handle.net/1853/27686.

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48

Renberg, Björn. "Fluorescence-based ligand assays for protein detection using affibody affinity proteins." Doctoral thesis, KTH, Skolan för bioteknologi (BIO), 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-3936.

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The detection and quantification of biomolecules, and proteins in particular, are of great interest since these molecules are of fundamental importance to our well-being. Body fluids, as for instance human blood, are well suited for sampling of protein levels. However, the complexity of the fluids and the low abundance of many of the interesting biomolecules makes detection and quantification difficult. This has spurred an interest into the development of many protein detection methods, and of these, ligand assays have proven particularly suitable. In this thesis, different types of ligand ass

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49

Harvey, Michael D. "Nanoscale protein analysis utilizing capillary electrophoresis and laser-induced fluorescence detection." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/NQ59213.pdf.

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50

Renberg, Björn. "Fluorescence-based ligand assays for protein detection using affibody affinity proteins /." Stockholm, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-3936.

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