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Relevant bibliographies by topics / HPTLC analysis

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Academic literature on the topic 'HPTLC analysis'

Author: Grafiati

Published: 5 June 2025

Last updated: 1 August 2025

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Journal articles on the topic "HPTLC analysis"

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Saibaba, S. V., and P. Shanmuga Pandiyan. "High Performance Thin Layer Chromatography: A Mini Review." Volume 2, Issue 4: October 2016 – December 2016 2, no. 4 (2016): 219–26. http://dx.doi.org/10.32463/rphs.2016.v02i04.43.

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High Performance Thin layer Chromatography (HPTLC) technique is a sophisticated and automated form of the thin-layer chromatography (TLC) with better and advanced separation efficiency and detection limits, and is often an excellent alternative to GC and HPLC. Applications of HPTLC include phytochemical and biomedical analysis, herbal drug quantification, active ingredient quantification, fingerprinting of formulations, and check for adulterants in the formulations. HPTLC is useful in detecting chemicals of forensic concern. Various advance techniques in reference to HPTLC like hyphenations in

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Edward Raju Gope, Shaik Munnisha Begum, Pujitha Prayer Anisetti, et al. "A Review of Principles, Applications, and Recent Developments in HPTLC and HPLC." Journal of Pharma Insights and Research 2, no. 6 (2024): 056–64. https://doi.org/10.69613/315vge42.

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High-Performance Thin Layer Chromatography (HPTLC) and High-Performance Liquid Chromatography (HPLC) represent fundamental analytical techniques in modern chemical analysis. These sophisticated separation methods have revolutionized the field of analytical chemistry through their precision, reliability, and versatility. HPTLC, an enhancement of traditional thin-layer chromatography, offers advantages such as minimal sample preparation, simultaneous analysis of multiple samples, and cost-effectiveness. It has found extensive applications in pharmaceutical analysis, natural product research, and

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Gray, Dean E., Dale Messer, Andrew Porter, et al. "Analysis of Flavonol Aglycones and Terpenelactones in Ginkgo biloba Extract: A Comparison of High-Performance Thin-Layer Chromatography and Column High-Performance Liquid Chromatography." Journal of AOAC INTERNATIONAL 90, no. 5 (2007): 1203–9. http://dx.doi.org/10.1093/jaoac/90.5.1203.

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Abstract Advancements in automated high-performance thin-layer chromatography (HPTLC) have made it feasible to assess its use for the quantitative analysis of marker compounds in botanical preparations. We report here the findings of method comparisons for the terpenelactones and flavonol aglycones by column high-performance liquid chromatography (HPLC) with evaporative light scattering and UV detection, and HPTLC with a scanning densitometer. For the HPTLC assay of terpenelactones, total bilobalide, ginkgolide A, and ginkgolide B consistently achieved &lt;70 of the total determined using

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Syeda, Rana Nikhat* Prof A.Ravinder Nath V.Rajesh Babu. "EXTRACTION AND CHARACTERIZATION OF 4- HYDROXYISOLEUCINE FROM TRIGONELLA FOENUM GRAECUM SEEDS." Indo American Journal of Pharmaceutical Sciences 04, no. 06 (2017): 1711–16. https://doi.org/10.5281/zenodo.822436.

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The objective of the present investigation is to extract, quantify and characterize the active constituent, 4- hydroxyisoleucine in Trigonella foenum graecum seeds using HPLC and HPTLC methods. Aqueous extract of crushed seeds of Trigonella foenum graecum was prepared and subjected to HPLC and HPTLC analysis. HPLC analysis was performed using C18 reverse phase column and Fluorescence detector with an elution gradient composed of 65 mmol Sodium acetate, l.5% tetrahydrofurane (pH 5.7) and methanol. HPTLC analysis was performed using semiautomatic TLC sampler Linomat V (CAMAG) controlled by WinCA

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B, Gunjal Sanket, and Dighe P. R. "Analysis of Herbal Drugs by HPTLC: A REVIEW." Asian Journal of Pharmaceutical Research and Development 10, no. 2 (2022): 125–28. http://dx.doi.org/10.22270/ajprd.v10i2.1056.

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Densitometric evaluation through HPTLC is very useful method for standardization of medicinal plants and other natural products, especially those used in different system of medicine. It has been approved as an authenticated method of analysis in several pharmacopeias, including the USP and the IP. Result from a number of medicinal plants studied in the laboratory have shown that, TLC- densitometry is more advantageous strategy than HPLC or GLC. Improvement in resolution, HPTLC is valuable tool for the investigation of herbal products with respect to different aspects of their quality. sensiti

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Fecka, Izabela, Adam Kowalczyk, and Wojciech Cisowski. "Phenolic Acids and Depsides from Some Species of the Erodium Genera." Zeitschrift für Naturforschung C 56, no. 11-12 (2001): 943–50. http://dx.doi.org/10.1515/znc-2001-11-1205.

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Abstract Six natural polyphenolic compounds, brevifolin carboxylic acid, brevifolin, ellagic acid, methyl gallate, gallic acid and protocatechuic acid have been isolated from the methanol extract of the whole plant of Erodium cicutarium (L.) L.’Herit. (Geraniaceae). Structures were determined by conventional methods of analysis and confirmed by MS and NMR spectral analysis. The distribution of these compounds in the other species of the Erodium genera (E. botrys, E. chium, E. ciconium, E. cicutarium, E. glutinosum subsp. dunense, E. gruinum, E. manescavi, E. pelargoniiflorum, E. petraeum ) wer

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K., Nagaraju, Mekala Sunil, Anusha M., Gopaiah Kurra Venkata, and Subbarao G. "Carvedilol, Tablets Assay Method Development and Validation by UV Spectroscopy, RP-HPLC and HPTLC." Trends in Pharmaceuticals and Nanotechnology (e-ISSN: 2582-4457) 2, no. 1 (2020): 37–47. https://doi.org/10.5281/zenodo.3632225.

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<em>Aim & Objectives : </em><em>The present study describes the development of a new rapid, simple, sensitive and reproducible RP-HPLC, HPTLC, UV Spectrophotometry method for the analysis of Carvedilol that offer certain advantages in its simplicity and sensitivity and applicable in routine analysis. Material & Methods: Carvedilol is polar in nature, reverse phase chromatography has been used and assay method is developed for UV-Spectroscopy, RP-HPLC, & HPTLC   & method validation is also done. Results & Discussion: The validated method was applied for the analysi

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Jug, Urška, Vesna Glavnik, Eva Kranjc, and Irena Vovk. "HPTLC–densitometric and HPTLC–MS methods for analysis of flavonoids." Journal of Liquid Chromatography & Related Technologies 41, no. 6 (2018): 329–41. http://dx.doi.org/10.1080/10826076.2018.1448690.

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Hussain, Muzammil, Kiran Aftab, Munawar Iqbal, et al. "Determination of Pesticide Residue in Brinjal Sample Using HPTLC and Developing a Cost-Effective Method Alternative to HPLC." Journal of Chemistry 2020 (September 18, 2020): 1–12. http://dx.doi.org/10.1155/2020/8180320.

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Two analytical techniques HPLC (high performance liquid chromatography) and HPTLC (high performance thin layer chromatography) were validated to reveal the quality and quantity of pesticide residues (organophosphorus, organochlorine, and pyrethroids) in brinjal samples collected from a local market of Faisalabad. The HPTLC methods showed linear behavior for standard samples and residue was in the range of 1–130 ng. The organochlorine (α-endosulfan) contaminates the samples at 4, 5, 9, and 10 weeks, and detected quantity was less than MRL (minimum residue level) of the FAO (Food and Agriculture

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Wulandari, Lestyo. "EVALUATION OF RE-USED HPTLC PLATE FOR QUALITATIVE AND QUANTITATIVE ANALYSIS." Indonesian Journal of Chemistry 6, no. 3 (2010): 338–40. http://dx.doi.org/10.22146/ijc.21743.

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The eficiency chromatography of HPTLC plate is better than conventional TLC plate but it more expensive. Due to the higher price of HPTLC plate compare to conventional TLC plate, the application of ";re-used"; HPTLC plate has been tested. After being used for analyzing samples, the plates were developed again with the mobile phase, then dried for 60 min before using for the next measurement. The qualitative chromatography profile showed that ";re-used"; HPTLC plate has less contaminant than new HPTLC plate and the RSD of the precision quantitative testing of the plates reused three times showe

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Dissertations / Theses on the topic "HPTLC analysis"

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Ammar, Amal [Verfasser]. "Analysis of drug substances by using new concepts of HPLC and development of some HPTLC methods / Amal Ammar." Berlin : Freie Universität Berlin, 2009. http://d-nb.info/1023956632/34.

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Narayan, Vicrant S. "HPLC analysis of nucleotides." Thesis, Queensland University of Technology, 2017. https://eprints.qut.edu.au/104440/1/Vicrant_Narayan_Thesis.pdf.

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This project developed a novel hydrophilic interaction chromatography method for the detection and identification of nucleotides. The method was validated by testing biological specimens; adult and neonatal saliva, and red and white blood cell extracts. The project discovered an interesting difference between the adult and neonatal saliva specimens. The neonatal saliva contained 5 additional nucleotides and the nucleotides were present in greater concentrations, when compared to the concentrations in adult saliva. With further optimisation this method may be applied to testing in Pathology ser

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Che, Isa Rosmahani. "Analysis of rubber accelerators by HPLC." Thesis, Loughborough University, 2009. https://dspace.lboro.ac.uk/2134/34256.

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The analysis of rubber accelerators particularly zinc dithiocarbamates (ZDTCs) was carried out by high performance liquid chromatography (HPLC) to develop an improved analytical method for the determination of rubber accelerators. Further understanding of their chemical and chromatographic behaviour has been established by investigating a few conventional as well as the latest reported methods, which include the pre-column derivatisation of ZDTCs to cobalt (III) complexes, direct determination by the addition of other protective agent and pre-column derivatisation of ZDTCs to copper (III) comp

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Walker, Roderick Bryan. "HPLC analysis and pharmacokinetics of cyclizine." Thesis, Rhodes University, 1995. http://hdl.handle.net/10962/d1003279.

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The investigations detailed in this dissertation have been conducted to address the paucity of pharmacokinetic information, in published literature, pertaining to cyclizine. The areas of investigation have included the selective quantitation of both cyclizine and its demethylated metabolite, norcyclizine in serum and urine, assessment of stability of both compounds in stored biological samples, dosage form analysis, dissolution rate testing of tablets, and bioavailability and pharmacokinetics following administration of an intravenous solution, and tablets to humans. High-performance liquid ch

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Westlake, James P. "The analysis of basic drugs by HPLC." Thesis, Loughborough University, 1991. https://dspace.lboro.ac.uk/2134/10517.

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Two methods for the high performance liquid chromatographic analysis of basic drugs have been studied. In each case the methods have concentrated on separating drugs of forensic interest, based on specially designed test solutions of analytes selected to represent most of the commonly encountered classes of drug compounds. In the first case, a HPLC method was developed on unmodified silica using an aqueous methanolic eluent of high pH. The buffer was prepared from two organic sulphonic acid amines, 3-(cyclohexylamino)-1- propanesulphonic acid (CAPS) and sodium 3-(cyclohexylamino)-2-hydroxy-1-

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Kavianpour, Keyhandokht. "Study of degradation of chlorophylls using DAD-HPLC and chemometrics." Thesis, University of Bristol, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.262839.

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Marr, J. G. D. "Computer-aided detection systems for HPLC : Development, assessment and application of digital techniques for peak purity validation in HPLC utilising photodiode array detection." Thesis, University of Bradford, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.384261.

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Hayteas, David Lawrence. "HPLC analysis of myoglobin tryptic peptides from selected species of cetaceans." PDXScholar, 1990. https://pdxscholar.library.pdx.edu/open_access_etds/4086.

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Due to the large gaps in the fossil record, the evolutionary history of the mammalian order Cetacea is incomplete and controversial. Increasingly researchers are utilizing molecular and biochemical procedures to supplement cetacean paleontology. One of these methods is the comparison of amino acid sequences of myoglobin among species of this order. since this method is time-consuming and expensive, an alternative procedure is desirable.

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Sher, Mohammed Nidhal Meena. "Extraction and HPLC analysis of potato sprout suppressant chemicals." Thesis, University of Glasgow, 2012. http://theses.gla.ac.uk/3454/.

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Abstract In the UK, up to six million tonnes of potatoes are produced annually and more than half of this production is stored for the fresh market and food processing. To maintain potato quality from sprouting, chlorpropham (CIPC) is currently used as the main sprout suppressant in commercial potato stores. Questions have been raised about the safety of application of this compound in potato stores due to increasing concern about the toxicity of its residue and degradation products mainly 3-chloroaniline (3-CA) on the potato tubers. To date, there is no realistic replacement of CIPC for inhib

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Macdonald, L. S. "Surfactant analysis by automatic ion-exchange separation and HPLC." Thesis, University of Strathclyde, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.369537.

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Books on the topic "HPTLC analysis"

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P, Henschel, Laubereau P. G, and Commission of the European Communities., eds. HPTLC applied to the analysis of the aquatic environment. Guyot, 1990.

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Zdeněk, Deyl, ed. HPLC in enzymatic analysis. 2nd ed. Wiley, 1998.

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Pasch, Harald. HPLC of polymers. Springer, 1998.

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Pasch, Harald. HPLC of polymers. Springer, 1997.

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Rossomando, Edward F., ed. HPLC in Enzymatic Analysis. John Wiley & Sons, Inc., 1998. http://dx.doi.org/10.1002/9780470110591.

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R, Macrae, ed. HPLC in food analysis. 2nd ed. Academic Press, 1988.

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Nollet, Leo M. L., 1948-, ed. Food analysis by HPLC. 2nd ed. Marcel Dekker, 2000.

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Szepesi, Gabór. HPLC in pharmaceutical analysis. CRC Press, 1990.

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Nollet, Leo M. L., 1948-, ed. Food analysis by HPLC. Marcel Dekker, 1992.

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Ghosh, Mantu Kumar. HPLC Methods on Drug Analysis. Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-76506-3.

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Book chapters on the topic "HPTLC analysis"

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Soni, Priyanka, Vishal Soni, Shivanee Vyas, Vaishali Raghuwanshi, and Rakhi Khabiya. "HPTLC Analysis of Food Spices." In Analysis of Food Spices. CRC Press, 2023. http://dx.doi.org/10.1201/9781003279792-16.

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Spangenberg, Bernd, Colin F. Poole, and Christel Weins. "Bioeffective-Linked Analysis in Modern HPTLC." In Quantitative Thin-Layer Chromatography. Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-10729-0_8.

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Jørgensen, A., K. Fredricson Overø, T. Aaes-Jørgensen, and J. V. Christensen. "Analysis of Thioxanthenes by HPTLC, HPLC, Capillary GC, and RIA." In BIOACTIVE ANALYTES, Including CNS Drugs, Peptides, and Enantiomers. Springer US, 1986. http://dx.doi.org/10.1007/978-1-4757-1892-8_15.

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Dhandhukia, Pinakin C., and Janki N. Thakker. "Quantitative Analysis and Validation of Method Using HPTLC." In High-Performance Thin-Layer Chromatography (HPTLC). Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-14025-9_12.

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Cimpoiu, Claudia. "HPTLC Hyphenated with FTIR: Principles, Instrumentation and Qualitative Analysis and Quantitation." In High-Performance Thin-Layer Chromatography (HPTLC). Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-14025-9_18.

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Ram, Mauji, M. Z. Abdin, M. A. Khan, and Prabhakar Jha. "HPTLC Fingerprint Analysis: A Quality Control for Authentication of Herbal Phytochemicals." In High-Performance Thin-Layer Chromatography (HPTLC). Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-14025-9_7.

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Kondakova, Tatiana, Nadine Merlet Machour, and Cécile Duclairoir Poc. "HPTLC-MALDI TOF MS Imaging Analysis of Phospholipids." In Neuromethods. Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-6946-3_12.

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Singaravelu, Anand, Kalaivani Arumugam, and Mettilda Manuel Swami Dorai. "Quantitative analysis and optimization of aloe emodin glycosides using HPLC and HPTLC techniques." In Recent Innovations in Sciences and Humanities. CRC Press, 2025. https://doi.org/10.1201/9781003606611-26.

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Singh, Shradhanjali, Shanti Bhushan Mishra, and Alok Mukerjee. "HPTLC Fingerprinting Analysis of Phytoconstituents from Indigenous Medicinal Plants." In Evidence Based Validation of Traditional Medicines. Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-15-8127-4_17.

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Hatziantoniou, Sophia, and Costas Demetzos. "Method of Simultaneous Analysis of Liposome Components Using HPTLC/FID." In Methods in Molecular Biology. Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60761-447-0_24.

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Conference papers on the topic "HPTLC analysis"

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Chia, Dian Ning, Fanyi Duanmu, Luca Mazzei, Eva Sorensen, and Maximilian O. Besenhard. "The Smart HPLC Robot: Fully Autonomous Method Development Guided by A Mechanistic Model Framework." In The 35th European Symposium on Computer Aided Process Engineering. PSE Press, 2025. https://doi.org/10.69997/sct.116643.

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Developing ultra- or high-performance liquid chromatography (HPLC) methods for analysis or purification requires significant amounts of material and manpower, and typically involves time-consuming iterative lab-based workflows. This work demonstrates in two case studies that an autonomous HPLC platform coupled with a mechanistic model that self-corrects itself by performing parameter estimation can efficiently develop an optimized HPLC method with minimal experiments (i.e., reduced experimental costs and burden) and manual intervention (i.e., reduced manpower). At the same time, this HPLC plat

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Batubara, Winda Rizki Pebrina, Tri Nugraha Susilawati, and Dono Indarto. "Analysis of Naringin and Hesperidin Compounds in Berastagi Sweet Orange (<i>Citrus Sinensis L</i>) Peel Extract." In 8th International Conference on Advanced Material for Better Future. Trans Tech Publications Ltd, 2025. https://doi.org/10.4028/p-aj6obu.

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Lipid metabolism is the process of breaking down triglycerides into fatty acids and glycerol, which are oxidized to provide energy. Peroxisome proliferation-activated receptor (PPAR)- alpha and adenosine monophosphate protein kinase (AMPK) are key proteins to increase endogenous lipolysis, which become important targets for weight loss management. Orange peels generally contain hesperidin and naringin compounds which can improve lipid metabolism through lipolysis, cholesterol regulation, inhibition of fat absorption, antioxidants and anti-inflammation. This study aimed to identify hesperidin a

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Yao, Zhiguo, Shen Guo, Jiaying Lin, Jichuan Zhang, Jie Ren, and Xianzheng Cui. "The Analysis of Low-voltage Substation Business Load Capacity Based On HPLC+HRF Dual-mode Communication Technology." In 2024 International Annual Conference on Complex Systems and Intelligent Science (CSIS-IAC). IEEE, 2024. https://doi.org/10.1109/csis-iac63491.2024.10919450.

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Li, Yuxian, Ying Qiu, Junguo Zhang, Ying Wang, and Shengxue Zhou. "Analysis of processing technology of vinegar-steamed Schisandra chinensis by PCA and PLS-DA combined with HPLC fingerprint." In The International Conference on Optical Communication and Optoelectronic Technology (OCOT 2024), edited by Mário F. Ferreira. SPIE, 2024. http://dx.doi.org/10.1117/12.3040845.

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Matherly, Ron M., Jian Jiao, David J. Blumer, and Jeffery S. Ryman. "Determination of Imidazoline and Amido-Amine Type Corrosion Inhibitors in Both Crude Oil and Produced Brine from Oilfield Production." In CORROSION 1995. NACE International, 1995. https://doi.org/10.5006/c1995-95543.

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Abstract The classical method for the determination of corrosion inhibitors in oilfield brines is the dye transfer method. Within this method are many variations which the analyst may use to determine the amount of corrosion inhibitor in either water or crude oil. These methods, however, suffer from many interferences which result in both false positive and negatives for corrosion inhibitor content. These methods essentially detect all amines as corrosion inhibitors. Improved high pressure liquid chromatography (HPLC) methods have been developed for the analysis of quaternary salt type corrosi

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Devina, Kezia Elian, Dono Indarto, and Tri Nugraha Susilawati. "Identification of Quercetin and Chrysin in Banana Peel Extract Using High Performance Liquid Chromatography and Liquid Chromatography Mass Spectrometer for Obesity Treatment." In 8th International Conference on Advanced Material for Better Future. Trans Tech Publications Ltd, 2025. https://doi.org/10.4028/p-6iz2xi.

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Pancreatic lipase plays an important role in converting triglyceride into long-chain fatty acids and glycerol, then becomes a therapeutic target for obesity treatment. Quercetin and chrysin are able to inhibit pancreatic lipase, which is potentially developed for obesity treatment. This study aimed to identify quercetin and chrysin derived from the methanol extract of raja and kepok banana peels using High Performance Liquid Chromatography (HPLC) and Liquid Chromatography Mass Spectrometer (LC-MS) for the development of obesity treatment. Raja and kepok banana peels were purchased from a fruit

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Ribeiro, M., E. Galli, J. A. L. Silva, I. Castanheira, A. Leufroy, and P. Jitaru. "Development and validation of a new analytical approach for simultaneous speciation analysis of Hg and Se in fishery products by HPLC-ICP-MS." In 7th IMEKOFOODS International Conference. IMEKO, 2025. https://doi.org/10.21014/tc23-2023.022.

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Argyropoulou, A., IK Tseti, E. Thoma, and LA Skaltsounis. "Analysis of Herbofix® herbal infusions and comparative study with traditional infusions employing HPTLC, HPLC-DAD and UPLC-MS." In 67th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA) in cooperation with the French Society of Pharmacognosy AFERP. © Georg Thieme Verlag KG, 2019. http://dx.doi.org/10.1055/s-0039-3399750.

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Hamad, Glena, Shakthi Sethuraman, and Kamaraj Raju. "HPTLC Fingerprinting Profile and GC-MS Analysis of Methanolic Fruit Extract of Akre’s Rhus coriaria (Sumac)." In 5th International Conference on Biomedical and Health Sciences. Cihan University-Erbil, 2024. http://dx.doi.org/10.24086/biohs2024/paper.1425.

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The study was aimed to determine the chemical components, present in the methanolic fruit extract of Rhus coriaria that was sourced from Akre, Kurdistan, Iraq, using high- performance thin-layer chromatography (HPTLC) and gas chromatography-mass spectrometry (GC-MS). A mobile phase system composed of toluene, ethyl acetate, and formic acid in the ratio of 7:3:0.5 (v/v/v) was used to perform the HPTLC fingerprint analysis on the methanolic extract. The results showed that at 254 nm, 366 nm, and 520 nm, the component at peak 4, 1, and 13 have the largest peak area percentages at 39.62%, 13.3%, a

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Моргунов, И. М., О. В. Евдокимова, and Н. П. Антонова. "APPLICATION OF THE HPTLC METHOD-DENSITOMETRY IN THE ANALYSIS OF BEARBERRY LEAVES." In ОТ БИОХИМИИ РАСТЕНИЙ К БИОХИМИИ ЧЕЛОВЕКА. Федеральное государственное бюджетное научное учреждение "Всероссийский научно-исследовательский институт лекарственных и ароматических растений", 2022. http://dx.doi.org/10.52101/9785870191041_200.

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Reports on the topic "HPTLC analysis"

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Lampi, Anna-Maija. Analysis of Tocopherols and Tocotrienols by HPLC. AOCS, 2011. http://dx.doi.org/10.21748/lipidlibrary.40389.

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Coffee, K. Analysis of TATB by HPLC-DAD Rev1.4. Office of Scientific and Technical Information (OSTI), 2021. http://dx.doi.org/10.2172/1811767.

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Hayteas, David. HPLC analysis of myoglobin tryptic peptides from selected species of cetaceans. Portland State University Library, 2000. http://dx.doi.org/10.15760/etd.5970.

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Annunziato, Dom. High-Performance Liquid Chromatography Analysis of Psilocybin-Containing Mushrooms: Key Considerations and Insights. GenTech Scientific LLC, 2023. http://dx.doi.org/10.61073/gentech.hplc.top.5.

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HPLC analysis of psilocybin-containing mushrooms is a complex process that requires careful attention to detail. In this scientific summary, Doma Nunzio1, MagicMyco C.S.O., offers insight and new answers to some old questions about these magic fungi. Based on his dedicated research and progress in the field, here are his top five things to keep in mind when conducting HPLC-UV analysis in reverse phase ‘RP Mode’ for psilocybin-containing mushrooms.

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Owens, J., A. Vu, and C. Koester. Analysis of Ethanolamines: Validation of Semi-Volatile Analysis by HPLC-MS/MS by EPA Method MS888. Office of Scientific and Technical Information (OSTI), 2008. http://dx.doi.org/10.2172/945589.

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Owens, J., and C. Koester. Analysis of Thiodiglycol: Validation of Semi-Volatile Analysis by HPLC-MS/MS by EPA Method MS777. Office of Scientific and Technical Information (OSTI), 2008. http://dx.doi.org/10.2172/945866.

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Annunziato, Dominick. HPLC Sample Prep and Extraction SOP v1.3 for Fungi. MagicMyco, 2023. http://dx.doi.org/10.61073/sopv1.3.08.11.2023.

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Abstract:

medicine, industry, and biotechnology. Fungi produce a wide range of bioactive compounds, such as alkaloids, antibiotics, antifungals, immunomodulators, anticancer agents, enzymes, and vitamins. However, these compounds are often locked inside the fungal cell wall, which is composed of chitin, a tough substance that is dif�icult to digest by humans1. Therefore, it is essential to have a good extraction technique that can break down the chitin and release the valuable compounds from the fungi, this is especially essential in the laboratory for accurate lab assays and potency determination durin

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Owens, J., A. Vu, and C. Koester. Analysis of Phosphonic Acids: Validation of Semi-Volatile Analysis by HPLC-MS/MS by EPA Method MS999. Office of Scientific and Technical Information (OSTI), 2008. http://dx.doi.org/10.2172/945524.

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Owens, J., and C. Koester. Analysis of Carbamate Pesticides: Validation of Semi-Volatile Analysis by HPLC-MS/MS by EPA Method MS666. Office of Scientific and Technical Information (OSTI), 2008. http://dx.doi.org/10.2172/945575.

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Docheva, Margarita, Yovcho Kochev, Desislava Kirkova, and Anna Stoilova. Validation of HPLC Method for Analysis of 5-hydroxymethyl-2-furfural in Tobacco Smoke. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, 2020. http://dx.doi.org/10.7546/crabs.2020.11.07.

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