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Journal articles on the topic 'HPTLC method'

Author: Grafiati

Published: 5 June 2025

Last updated: 1 August 2025

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1

Divya, Bhondve* Rasika Kamthe Samruddhi Zagade Biradar S. M. "HPTLC Method Development and Validation: An Overview." International Journal of Pharmaceutical Sciences 2, no. 12 (2024): 216–22. https://doi.org/10.5281/zenodo.14264166.

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This review composition gives knowledge of about HPTLC- grounded logical system development and confirmation parameter in agreement to practical evaluation. It meets norms and minimizes crimes and disquisition. This review composition helps to choose stylish mobile phase and gives guidelines for the good confirmation practice and understand the way of logical procedure. High- Performance Thin Subcaste Chromatography (HPTLC) is an enhanced and automated system of thin- subcaste chromatography TLC) that offers superior separation performance and discovery limits and is constantly a great cover f

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Eissa, Maya S., Eman Darweish, Mohammed R. Elghobashy, and Mostafa A. Shehata. "Chromatographic Separation of the Novel Hypoglycemic Drug Mitiglinide in its Bulk Powder and Pharmaceutical Formulation: Forced Degradation and Validated Stability-Indicating HPTLC/Densitometry and HPLC/UV Methods." Journal of AOAC INTERNATIONAL 103, no. 3 (2020): 736–42. http://dx.doi.org/10.1093/jaocint/qsz004.

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Abstract Background Mitiglinide (MTG) is one of meglitinides group which are used for treatment of type two diabetes mellitus. Objective Mitiglinide (MTG) is a novel oral hypoglycemic drug. The present work adopts two stability-indicating chromatographic methods for determination of MTG after being exposed to forced degradation using 4 M methanolic HCl for 12 h. Methods The first method is HPTLC/densitometry using methanol:chloroform:acetic acid (5:2.5:0.3 by volume) as the eluting system and silica gel 60 GF254 as the stationary phase; the separated bands were then scanned at 220 nm. The seco

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Bollepaka, Srilatha Santhosh Illendula. "STABILITY INDICATING METHOD DEVELOPMENT OF RIMONABANT AND IT's VALIDATION BY RP-HPLC AND HPTLC METHOD." INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES 05, no. 06 (2018): 5602–10. https://doi.org/10.5281/zenodo.1297120.

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A new simple, accurate, rapid, precise, reproducible and cost effective HPLC and HPTLC method for the quantitative estimation of Rimonabant in bulk and pharmaceutical dosage form. In Method using HPTLC, chromatograms were developed in a mobile phase of 2 mM Sodium dihydrogen orthophosphate (NaH2PO4)-Acetonitrile (38:62) for approximately 25 mins and detected at λmax of 303 nm. Accuracy was found to be in the range of 98.31-102.25 % whereas intraday precision was found to be in the range of 0.03 to 1.60%. Interday precision was found to be 1.36, 0.74 and 0.31 at LQC, MQC and HQC, respect

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Damle, M. C., and K. K. Pardeshi. "Development of Analytical Method to Monitor Dissolution of Bepotastine Besilate Tablet." Journal of Drug Delivery and Therapeutics 9, no. 4 (2019): 251–56. http://dx.doi.org/10.22270/jddt.v9i4.3037.

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Bepotastine Besilate is an anti-histaminic drug and it is marketed as tablet of strength 10mg. In this study an attempt is made to monitor the dissolution of Bepotastine Besilate tablet. Dissolution study was done for marketed sample using phosphate buffer 6.8, phosphate buffer 4.5 and 0.1 N HCl as dissolution media. Samples were analysed using UV spectrophotometer, HPLC and HPTLC. Detection wavelength selected was 226nm. A chromatographic separation is achieved on a C18 column with a mobile phase consisting of acetonitrile, water with isocratic elution with flow rate 1ml/min. Solvents used fo

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K., Nagaraju, Mekala Sunil, Anusha M., Gopaiah Kurra Venkata, and Subbarao G. "Carvedilol, Tablets Assay Method Development and Validation by UV Spectroscopy, RP-HPLC and HPTLC." Trends in Pharmaceuticals and Nanotechnology (e-ISSN: 2582-4457) 2, no. 1 (2020): 37–47. https://doi.org/10.5281/zenodo.3632225.

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<em>Aim & Objectives : </em><em>The present study describes the development of a new rapid, simple, sensitive and reproducible RP-HPLC, HPTLC, UV Spectrophotometry method for the analysis of Carvedilol that offer certain advantages in its simplicity and sensitivity and applicable in routine analysis. Material & Methods: Carvedilol is polar in nature, reverse phase chromatography has been used and assay method is developed for UV-Spectroscopy, RP-HPLC, & HPTLC   & method validation is also done. Results & Discussion: The validated method was applied for the analysi

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Gray, Dean E., Dale Messer, Andrew Porter, et al. "Analysis of Flavonol Aglycones and Terpenelactones in Ginkgo biloba Extract: A Comparison of High-Performance Thin-Layer Chromatography and Column High-Performance Liquid Chromatography." Journal of AOAC INTERNATIONAL 90, no. 5 (2007): 1203–9. http://dx.doi.org/10.1093/jaoac/90.5.1203.

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Abstract Advancements in automated high-performance thin-layer chromatography (HPTLC) have made it feasible to assess its use for the quantitative analysis of marker compounds in botanical preparations. We report here the findings of method comparisons for the terpenelactones and flavonol aglycones by column high-performance liquid chromatography (HPLC) with evaporative light scattering and UV detection, and HPTLC with a scanning densitometer. For the HPTLC assay of terpenelactones, total bilobalide, ginkgolide A, and ginkgolide B consistently achieved &lt;70 of the total determined using

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Saad, Javed Shaikh*, and Daunde Swapnil. "A REVIEW ON METHOD DEVELOPMENT AND VALIDATION OF STABILITY INDICATING UV METHOD FOR VILDAGLIPTIN." World Journal of Pharmaceutical Science and Research 4, no. 2 (2025): 899–909. https://doi.org/10.5281/zenodo.15302742.

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The present study describes the development of a new rapid, simple, sensitive and reproducible UV Spectrophotometry RP-HPLC, HPTLC method for the analysis of Vildagliptin that offer certain advantages in its simplicity and sensitivity and applicable in routine analysis. Vildagliptin is an antidiabetic agent, belongs to the dipeptidyl peptidase IV (DPP-4) inhibitors. The method employs measurement of absorbance at the wavelength of maximum absorptions of vildagliptin using water as a solven. In order to develop a RP-HPLC, HPTLC, UV effective most of the effect should be spent in method developm

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8

Kapase, Sachin N. "ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF COMBINATION OF ANTI-ASTHMATIC DRUGS MONTELUKAST AND DOXOFYLLINE." Journal of Medical pharmaceutical and allied sciences 11, no. 6 (2021): 86–91. http://dx.doi.org/10.22270/jmpas.v10i6.1940.

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For qualitative and quantitative analysis, various analytical techniques are available such as Ultraviolet (UV) Spectrophotometry, High-performance liquid chromatography (HPLC), High-performance thin layer chromatography (HPTLC). As per literature survey, there are some UV, HPLC, Ultra-Performance Liquid Chromatography (UPLC) and HPTLC analytical methods are developed for Montelukast and Doxofylline individually and in a combination with other drugs too, since yet there are no significant stability studies indicating HPLC method reported for Montelukast and Doxofylline combinations. In the cur

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Ghante, M. R., S. D. Sawant, M. Undre, S. G. Jagtap, P. Kulkarni, and V. S. Nikam. "RP-HPLC AND HPTLC METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF DOLUTEGRAVIR IN BULK AND TABLET DOSAGE FORM." INDIAN DRUGS 56, no. 05 (2019): 30–38. http://dx.doi.org/10.53879/id.56.05.11288.

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Dolutegravir (DTG) is a second-generation integrase strand transfer inhibitor (INSTI), antiretroviral drug. RP-HPLC and HPTLC methods have been developed and validated for DTG in bulk and tablet dosage form. In HPLC method, analysis was carried out on C-18 column using a mixture of acetonitrile: phosphate buffer (pH 7.0) in the ratio of (90:10 V/V) as mobile phase at the flow rate 1 mL/min at 261 nm. The method was found to be linear in the concentration range 10-70 μg/mL. Peak for DTG was observed at 2.8 ± 0.1 minutes. For HPTLC method proper resolution was achieved with toluene: methanol: fo

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Sagar, Uttam Kumbhar*, and Dounde Swapnil. "A REVIEW ON METHOD DEVELOPMENT AND VALIDATION OF STABILITY INDICATING UV METHOD FOR CARVEDILOL." World Journal of Pharmaceutical Science and Research 4, no. 2 (2025): 748–57. https://doi.org/10.5281/zenodo.15274436.

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The present study describes the development of a new rapid, simple, sensitive and reproducible UV Spectrophotometry RP-HPLC, HPTLC method for the analysis of Carvedilol that offer certain advantages in its simplicity and sensitivity and applicable in routine analysis. It also describes the development of validation work as per ICH guidelines recommended by the Food and Drug Administration (FDA) of the United States. In order to develop a RP-HPLC, HPTLC, UV effective most of the effect should be spent in method development and optimization as this will improve the final method performance. A we

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Agrawal, Himani, Neeraj Kaul, A. R. Paradkar, and K. R. Mahadik. "HPTLC method for guggulsterone." Journal of Pharmaceutical and Biomedical Analysis 36, no. 1 (2004): 23–31. http://dx.doi.org/10.1016/j.jpba.2004.04.013.

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Agrawal, Himani, Neeraj Kaul, A. R. Paradkar, and K. R. Mahadik. "HPTLC method for guggulsterone." Journal of Pharmaceutical and Biomedical Analysis 36, no. 1 (2004): 33–41. http://dx.doi.org/10.1016/j.jpba.2004.04.014.

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Kumari Jaiswal, Pooja, and Vandana Jain. "Novel HPTLC Method for Estimation of Fluocinonide." International Journal of Science and Research (IJSR) 11, no. 11 (2022): 302–5. http://dx.doi.org/10.21275/sr221102134447.

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Hussain, Muzammil, Kiran Aftab, Munawar Iqbal, et al. "Determination of Pesticide Residue in Brinjal Sample Using HPTLC and Developing a Cost-Effective Method Alternative to HPLC." Journal of Chemistry 2020 (September 18, 2020): 1–12. http://dx.doi.org/10.1155/2020/8180320.

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Two analytical techniques HPLC (high performance liquid chromatography) and HPTLC (high performance thin layer chromatography) were validated to reveal the quality and quantity of pesticide residues (organophosphorus, organochlorine, and pyrethroids) in brinjal samples collected from a local market of Faisalabad. The HPTLC methods showed linear behavior for standard samples and residue was in the range of 1–130 ng. The organochlorine (α-endosulfan) contaminates the samples at 4, 5, 9, and 10 weeks, and detected quantity was less than MRL (minimum residue level) of the FAO (Food and Agriculture

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Abbas, Samah S., Maha A. Hegazy, Hassan A. M. Hendawy, Soheir A. Weshahy, and May H. Abdelwahab. "Resolution and Quantitation of Triamcinolone Acetonide and Its Coformulated Drug in the Presence of Its Impurities and Degradation Products by HPTLC and HPLC." Journal of AOAC INTERNATIONAL 101, no. 4 (2018): 981–91. http://dx.doi.org/10.5740/jaoacint.16-0422.

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Abstract Two specific, sensitive, and precise stability-indicating chromatographic methods have been developed for the determination of triamcinolone acetonide (TMC) and its coformulated drug, econazole nitrate (ECZ), in the presence of TMC impurities and degradation products. The first method was based on HPTLC-spectrodensitometry in which resolution and quantitation was achieved by using silica gel 60 F254 HPTLC plates and an ethyl acetate–tetrahydrofuran–ammonia mobile phase (10.0 + 7.0 + 0.1, v/v/v). The second method was a reversed-phase HPLC method in which separation was achieved using

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B, Gunjal Sanket, and Dighe P. R. "Analysis of Herbal Drugs by HPTLC: A REVIEW." Asian Journal of Pharmaceutical Research and Development 10, no. 2 (2022): 125–28. http://dx.doi.org/10.22270/ajprd.v10i2.1056.

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Densitometric evaluation through HPTLC is very useful method for standardization of medicinal plants and other natural products, especially those used in different system of medicine. It has been approved as an authenticated method of analysis in several pharmacopeias, including the USP and the IP. Result from a number of medicinal plants studied in the laboratory have shown that, TLC- densitometry is more advantageous strategy than HPLC or GLC. Improvement in resolution, HPTLC is valuable tool for the investigation of herbal products with respect to different aspects of their quality. sensiti

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Alam, Prawez, Essam Ezzeldin, Muzaffar Iqbal, et al. "Determination of Delafloxacin in Pharmaceutical Formulations Using a Green RP-HPTLC and NP-HPTLC Methods: A Comparative Study." Antibiotics 9, no. 6 (2020): 359. http://dx.doi.org/10.3390/antibiotics9060359.

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In this work; delafloxacin (DLFX) was determined using a validated green RP-HPTLC and NP-HPTLC methods in commercial tablets and in-house developed solid lipid nanoparticles (SLNs). RP-HPTLC determination of DLFX was performed using “RP-18 silica gel 60 F254S HPTLC plates”. However; NP-HPTLC estimation of DLFX was performed using “silica gel 60 F254S HPTLC plates”. For a green RP-HPTLC method; the ternary combination of ethanol:water:ammonia solution (5:4:2 v/v/v) was used as green mobile phase. However; for NP-HPTLC method; the ternary mixture of ethyl acetate: methanol: ammonia solution (5:4

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Narwade, Shital, Hemalata Wadkar, Aishwarya Ghanawat, and Krutika Nilwani. "A Comprehensive Review on HPTLC Method Development." International Journal of Research and Review 12, no. 2 (2025): 71–83. https://doi.org/10.52403/ijrr.20250209.

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High-Performance Thin-Layer Chromatography (HPTLC) is a widely utilized analytical technique that offers significant advantages in the qualitative and quantitative analysis of a variety of chemical substances. This review aims to provide a comprehensive overview of HPTLC method development, focusing on its principles, instrumentation, key parameters, and applications. The paper discusses the critical steps involved in HPTLC method development, including selection of stationary phases, mobile phases, detection methods, and optimization of experimental conditions to achieve high resolution, sens

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Alqarni, Mohammed H., Faiyaz Shakeel, Tariq M. Aljarba, et al. "Determination of Colchicine in Pharmaceutical Formulations, Traditional Extracts, and Ultrasonication-Based Extracts of Colchicum autumnale Pleniflorum (L.) Using Regular and Greener HPTLC Approaches: A Comparative Evaluation of Validation Parameters." Plants 11, no. 13 (2022): 1767. http://dx.doi.org/10.3390/plants11131767.

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In the literature, there is a scarcity of greener analytical approaches for colchicine (CLH) analysis. As a result, efforts were made in this study to develop and validate a greener reversed-phase high-performance thin-layer chromatography (HPTLC) technique for CLH analysis in traditional extracts (TE) and ultrasonication-based extracts (UBE) of commercial Unani formulations, commercial allopathic formulations, and Colchicum autumnale Pleniflorum (L.) obtained from Egypt and India. This new technique was compared to the regular normal-phase HPTLC method. The greenness profile of both methods w

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Rajendran, Jayaprakasam, Anita Elizabeth Chacko, Tresa Thomas, Neethu Johnson, and Kochupapy Ravi Thengungal. "RP-HPLC AND HPTLC METHODS FOR THE ESTIMATION OF LUPEOL IN. HEMIDESMUS INDICUS LINN. ROOT EXTRACTS AND POLYHERBAL FORMULATION USING STANDARD MA." INDIAN DRUGS 57, no. 07 (2020): 40–46. http://dx.doi.org/10.53879/id.57.07.11375.

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The roots of Hemidesmus indicus, belonging to the family Ascelpidaceae, are used as blood purifier, antileucorrhoeic, galactogenic, antidiarrhoeal, antirheumatic, antisyphilitic, febrifuge and possesses chemical constituents like triterpenoids (lupeol), flavonoids, glycosides and tannins. Roots were dried and extracted with petroleum ether, chloroform and methanol by using Soxhlet apparatus. Two simple and sensitive chromatographic methods, namely, HPTLC and HPLC, were developed for determination of lupeol from the extract of H. indicus and its polyherbal formulation and validated according to

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Kalyani, Ashok Patil*1 Shailesh B. Patil2. "To Develop And Validate Stability Indicating Assay Of Diloxanide Furoate By Using UV And HPLC And HPTLC Method For Pharmaceutical Dosage Form." International Journal in Pharmaceutical Sciences 2, no. 6 (2024): 498–517. https://doi.org/10.5281/zenodo.11543189.

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The study aimed to establish stability assays for Diloxanide Furoate using both High-Performance Liquid Chromatography (HPLC) and High-Performance Thin-Layer Chromatography (HPTLC) methods, covering crucial aspects of method development and validation. Detailed specifications were provided for the HPLC and HPTLC systems to ensure proper setup and functioning. Chromatographic conditions were optimized, including parameters like stationary phase, mobile phase composition, flow rate, and detection wavelength, to achieve optimal separation and detection of Diloxanide Furoate. The identity of pure

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Khadse, Saurabh C., Shailesh S. Chalikwar, Sandip D. Firke, Harshal Ramkrishna Baviskar, and Akshay D. Umale. "A Concise Review Based on Analytical Method Development and Validation of Apremilast in Bulk and Marketed Dosage Form." Journal of Drug Delivery and Therapeutics 10, no. 2-s (2020): 175–82. http://dx.doi.org/10.22270/jddt.v10i2-s.4012.

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Apremilast is used for treatment of psoriasis and psoriatic arthritis. It may also be beneficial for other inflammatory diseases relevant to the immune system. The drug functions as a selective enzyme phosphodiesterase 4 (PDE4) inhibitor and avoids the spontaneous development of TNF-alpha from human synovial rheumatoid cells. The present review assesses the different approaches for evaluation of apremilast in bulk material as well as different formulations. A concise review consists of compile and discuss about over 30 methods for analysing apremilast in the biological matrices, the samples of

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Barakaeva, Dildora, Nuritdin Mukarramov, Anarbay Babekov, Lola Zhamolova, and Maksatbek Turatbekov. "Qualitative and quantitative determination of ferulic acid by HPTLC and HPLC methods in root resin of Ferula Tadshikorum." E3S Web of Conferences 537 (2024): 05021. http://dx.doi.org/10.1051/e3sconf/202453705021.

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Fast, simple, accurate, specific and reliable methods of HPTLC and HPLC have been developed and proposed, which can be used for the analysis of ferulic acid in plant extracts. The HPTLC method is economical because it uses a very small number of mobile phases that effectively dissolve ferulic acid, and the sample purification procedure associated with it is minimal. The developed methods can be widely used to determine the quality of Ferula tadshikorum resin and formulations containing it.

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Patel, Rashmin B., Mrunali R. Patel, Madhira B. Shankar, and Kashyap K. Bhatt. "Simultaneous Determination of Alprazolam and Fluoxetine Hydrochloride in Tablet Formulations by High-Performance Column Liquid Chromatography and High-Performance Thin-Layer Chromatography." Journal of AOAC INTERNATIONAL 92, no. 4 (2009): 1082–88. http://dx.doi.org/10.1093/jaoac/92.4.1082.

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Abstract This paper describes validated HPLC and HPTLC methods for simultaneous determination of alprazolam (ALP) and fluoxetine hydrochloride (FXT) in pure powder and formulation. The HPLC separation was achieved on a Nucleosil C8 column (150 mm length, 4.6 mm id, 5 m particle size) using acetonitrilephosphate buffer pH 5.5 (45 + 55, v/v) as the mobile phase at a flow rate of 1.0 mL/min at ambient temperature. The HPTLC separation was achieved on an aluminum-backed layer of silica gel 60F254 using acetonetolueneammonia (6.0 + 3.5 + 0.5, v/v/v) as the mobile phase. Quantification in the HPLC m

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Zaazaa, Hala E., Rasha Abdel-Ghany, M. Abdelkawy, and Mahmoud Sayed. "Development and Validation of Two Robust Stability-Indicating Chromatographic Methods for Determination of Metolazone in Drug Substance and Pharmaceutical Dosage Form in the Presence of Its Degradation Products and Characterization of Main Degradation Products Based on LC-MS." Journal of Chromatographic Science 58, no. 3 (2019): 251–61. http://dx.doi.org/10.1093/chromsci/bmz110.

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Abstract Two robust and selective stability-indicating chromatographic methods were developed and validated for the determination of metolazone in drug substance and pharmaceutical dosage form in the presence of its degradation products. The HPLC method employed a Kromasil C18 (250 × 4.6,5 μm) column and a mobile phase of acetonitrile: 0.2% orthophosphoric acid (32:68 v/v) at a flow rate 2 mL/min and detection at 238 nm. The separation was performed in HPLC isocratic mode. The robustness of the suggested method was assessed using the Plackett–Burman design, parameters affecting system suitabil

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Rakesh Shrirang Shivatare, Dheeraj Hanumansingh Nagore, and Rakesh kumar Jat. "A rapid HPTLC method to estimate ximenynic acid in semisolid dosage formulation." World Journal of Biology Pharmacy and Health Sciences 13, no. 2 (2023): 080–88. http://dx.doi.org/10.30574/wjbphs.2023.13.2.0085.

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A novel HPTLC analytical technique and validation study were developed to determine the concentration of ximenynic acid (XMA) in semisolid dosage formulations (SDF) such as cream, gel, lotion, etc. The procedure makes use of reverse-phase high-performance liquid chromatography (HPLC). This study presents the first report of sensitive, selective, precise and robust HPTLC method, which has been developed and validated for quantification of the XMA from pharmaceutical formulation. The chromatographic development was carried out on HPTLC plates precoated with silica gel 60 F254 using a mixture of

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Rakesh, Shrirang Shivatare, Hanumansingh Nagore Dheeraj, and kumar Jat Rakesh. "A rapid HPTLC method to estimate ximenynic acid in semisolid dosage formulation." World Journal of Biology Pharmacy and Health Sciences 13, no. 2 (2023): 080–88. https://doi.org/10.5281/zenodo.7948605.

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A novel HPTLC analytical technique and validation study were developed to determine the concentration of ximenynic acid (XMA) in semisolid dosage formulations (SDF) such as cream, gel, lotion, etc. The procedure makes use of reverse-phase high-performance liquid chromatography (HPLC). This study presents the first report of sensitive, selective, precise and robust HPTLC method, which has been developed and validated for quantification of the XMA from pharmaceutical formulation. The chromatographic development was carried out on HPTLC plates precoated with silica gel 60 F254 using a mixture of

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Ganesh, Tiwari* Viral Maheshwari Rohan Shakywanshi Rukhsar khan Dr. Bhoomi Patel. "Development of UV Spectroscopic Method of Dapagliflozin with Some Validation Parameter." International Journal of Pharmaceutical Sciences 3, no. 5 (2025): 42–54. https://doi.org/10.5281/zenodo.15317978.

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This research compiles information on the development of analytical methods for estimating Dapagliflozin, which will be valuable for future research on this drug and its impurities. The literature survey covers various analytical methods such as UV, RP-HPLC, HPTLC, and other techniques reported for Dapagliflozin, both alone and in combination with other drugs. The analysis of published data indicates that only a UV spectroscopic method (calibration curve method) has been reported for estimating Dapagliflozin in fixed-dose combination. Each technique is evaluated for its sensitivity, specificit

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Mohammed, Idrees H.* Sunitha P. G. Deattu N. Jawaharsamuvel R. "Rupatadine: A Review On Analytical Method Development And Validation For Quantification Of Bulk And Pharmaceutical Dosage Form By Liquid Chromatography." International Journal of Pharmaceutical Sciences 2, no. 11 (2024): 15–26. https://doi.org/10.5281/zenodo.14024347.

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Through its interaction with certain receptors, rupatadine, a non-sedative, selective, and long-acting H1 antihistamine, has antagonistic PAF effects. We have conducted a thorough literature review of numerous journals pertaining to analytical and pharmaceutical chemistry. Additionally, we have examined instrumental analytical methods developed and employed for the purpose of identifying drugs in bulk pharmaceuticals, formulations, and biological fluids, either individually or in combination with other drugs. The most recent analytical techniques, such as liquid chromatography, RP HPLC, HPTLC,

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Alam, Prawez, Faiyaz Shakeel, Wael A. Mahdi, et al. "Determination of Gefitinib Using Routine and Greener Stability-Indicating HPTLC Methods: A Comparative Evaluation of Validation Parameters." Processes 10, no. 4 (2022): 762. http://dx.doi.org/10.3390/pr10040762.

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“High-performance thin-layer chromatography (HPTLC)” methods for gefitinib (GFT) estimation are scarce in the literature. In addition, greener analytical techniques for GFT estimation are also lacking in the literature. Accordingly, an attempt was undertaken to invent and validate a sensitive and greener normal-phase HPTLC method for GFT analysis in commercial tablets in comparison to the routine normal-phase HPTLC method. The greenness index for both methods was assessed using “Analytical GREENness (AGREE)” methodology. GFT detection was carried out using both methods at 332.0 nm. In the 30–7

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Aditi, Gade* Prasanna Datar. "A Review on Analytical Method Development for Drug Sitagliptin." International Journal of Pharmaceutical Sciences 3, no. 4 (2025): 2713–21. https://doi.org/10.5281/zenodo.15263891.

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The Sitagliptin offer significant therapeutic benefits in managing type 2 diabetes mellitus (T2DM) and associated cardiovascular risks. The development of accurate, precise, and validated analytical methods is crucial for ensuring their efficacy, safety, and regulatory compliance. This review explores various analytical techniques, including High-Performance Liquid Chromatography (HPLC), Reverse Phase HPLC (RP- HPLC), High-Performance Thin Layer Chromatography (HPTLC), Liquid Chromatography-Mass Spectrometry (LC-MS/MS), and UV spectrophotometry, highlighting their advantages, challenges, and v

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Patel, Bhavesh H., Bhanubhai N. Suhagia, Madhabhai M. Patel, and Jignesh R. Patel. "Simultaneous Estimation of Pantoprazole and Domperidone in Pure Powder and a Pharmaceutical Formulation by High-Perfomance Liquid Chromatography and High-Performance Thin-Layer Chromatography Methods." Journal of AOAC INTERNATIONAL 90, no. 1 (2007): 142–46. http://dx.doi.org/10.1093/jaoac/90.1.142.

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Abstract This paper describes validated high-performance liquid chromatography (HPLC) and high-performance thin-layer chromatography (HPTLC) methods for the simultaneous estimation of pantoprazole (PANT) and domperidone (DOM) in pure powder and capsule formulations. The HPLC separation was achieved on a Phenomenex C18 column (250 mm id, 4.6 mm, 5 μm) using 0.01 M, 6.5 pH ammonium acetate buffer-methanol-acetonitrile (30 + 40 + 30, v/v/v, pH 7.20) as the mobile phase at a flow rate of 1.0 mL/min at ambient temperature. The HPTLC separation was achieved on an aluminum-backed layer of silica gel

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Patel, Rashmin B., Madhira B. Shankar, Mrunali R. Patel, and Kashyap K. Bhatt. "Simultaneous Estimation of Acetylsalicylic Acid and Clopidogrel Bisulfate in Pure Powder and Tablet Formulations by High-Performance Column Liquid Chromatography and High-Performance Thin-Layer Chromatography." Journal of AOAC INTERNATIONAL 91, no. 4 (2008): 750–55. http://dx.doi.org/10.1093/jaoac/91.4.750.

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Abstract This paper describes validated high-performance column liquid chromatographic (HPLC) and high-performance thin-layer chromatographic (HPTLC) methods for simultaneous estimation of acetylsalicylic acid (ASA) and clopidogrel bisulfate (CLP) in pure powder and formulations. The HPLC separation was achieved on a Nucleosil C8 column (150 mm length 4.6 mm id, 5m particle size) using acetonitrilephosphate buffer, pH 3.0 (55 + 45, v/v) mobile phase at a flow rate of 1.0 mL/min at ambient temperature. The HPTLC separation was achieved on an aluminum-backed layer of silica gel 60F254 using ethy

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Vijendra, P. Rathod Rahul S. Wani Saurabh C. Khadse Atul A.Shirkhedkar. "A CONCISE REVIEW ON ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF OLANZAPINE." INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES o6, no. 05 (2019): 8927–47. https://doi.org/10.5281/zenodo.2669427.

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<em>Olanzapine (OLZ) is an atypical antipsychotic  agent is and different antipsychotic agent  medications like Carbamazepine, Fluoxetine hydrochloride, Simvastatin, Clozapine, paliperidone, Quetiapine, several beta blocker, Risperidone, 9-Hydroxyrisiperidone, Demethylolanzapine, Aripiprazole, Orphenadrine, 1,2 Naphthoquinone, P-dimethylamino Benzaldehyde, Cerium sulphate, N-bromosulphinimide. The present investigation assesses the various approaches for analysis of OLZ in bulk drug as well as their pharmaceutical formulations.</em> <em>A concise survey states the collection and outl

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Abdel Hameed, Eman A., Zaitona A. Abd El-Naby, Alaa El Gindy, et al. "Two Eco-Friendly Chromatographic Methods Evaluated by GAPI for Simultaneous Determination of the Fluoroquinolones Moxifloxacin, Levofloxacin, and Gemifloxacin in Their Pharmaceutical Products." Separations 9, no. 11 (2022): 330. http://dx.doi.org/10.3390/separations9110330.

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In this paper, novel green HPLC and HPTLC chromatographic methods were developed for the concurrent determination of moxifloxacin, levofloxacin, and gemifloxacin in bulk and pharmaceutical products. The green HPLC method was used on Thermo C18 (4.6 × 250 mm, 5 µm). By mixing ethanol and 20 mM sodium dihydrogen phosphate dihydrate (pH 5) in a ratio of 25:75, v/v, the mobile phase was created using isocratic elution. The flow rate was 1 mLmin−1. The studied antibiotics were separated well within 9.5 min. The green HPTLC method was used on coated HPTLC aluminum sheets with Silica gel 60 F254 usin

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Emam, Aml A., Ibrahim A. Naguib, Eman S. Hassan, and Eglal A. Abdelaleem. "Development and Validation of RP-HPLC and an Ecofriendly HPTLC Method for Simultaneous Determination of Felodipine and Metoprolol Succinate, and their Major Metabolites in Human Spiked Plasma." Journal of AOAC INTERNATIONAL 103, no. 4 (2020): 966–71. http://dx.doi.org/10.1093/jaoacint/qsz040.

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Abstract Background Felodipine is a calcium channel blocker used together with metoprolol succinate for treatment of hypertension. Objective Two chromatographic methods were developed for simultaneous determination of felodipine (FEL) and metoprolol succinate (MET), and their major metabolites, dehydrofelodipine and metoprolol acid, respectively. Methods The first method was RP-HPLC which comprised separation of the studied components by gradient elution using a Phenomenex C8 column and a mobile phase composed of water (adjusted to pH 3.5 with o-phosphoric acid)–acetonitrile – methanol (45:40:

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K, Sonia, Manikandan K, Hamunyare Ndwabe, Peddamadi Bhavya Sree, and Lakshmi KS. "Method Development and Validation of simultaneous estimation for Amlodipine besylate and Olmesartan medoxomil by HPTLC method." International Journal of Research in Pharmaceutical Sciences 9, no. 1 (2018): 201. http://dx.doi.org/10.26452/ijrps.v9i1.1246.

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The present work deals with method development and analytical validation of a novel, precise and accurate HPTLC methods for the simultaneous estimation of Amlodipine besylate and Olmesartan medoxomil. Literature review has shown that High performance liquid chromatography and UV-Visible Spectroscopy methods have been reported for the estimation of these drugs, but no HPTLC method has been done, thus this study had to be done. A analytical method development for the simultaneous estimation of Amlodipine besylate and Olmesartan medoxomil was developed. For simultaneous HPTLC method, the analytic

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Hegazy, Maha A., May H. Abdelwahab, Hassan A. M. Hendawy, Soheir A. Weshahy, and Samah S. Abbas. "Selective and Sensitive Chromatographic Methods for Determination of a Co-Formulated Binary Mixture in Antibacterial Eye Drops and Aqueous Humor in the Presence of Their Degradation Products and Potential Impurities." Journal of Chromatographic Science 58, no. 1 (2020): 37–52. http://dx.doi.org/10.1093/chromsci/bmz107.

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Abstract Prednisolone acetate (PDN) is a corticosteroid anti-inflammatory liable to degradation under different conditions and used with antibiotics in eye drops. Two selective stability-indicating separation techniques were developed for simultaneous determination of PDN and moxifloxacin HCl (MXF) binary mixture in pure forms, ophthalmic formulation, in the presence of PDN impurities and in the presence of their degradation products. The first method was based on HPTLC separation using silica gel 60 F254 HPTLC plates, and a developing system of toluene: ethyl acetate: methanol: ammonia (5.0:

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Litewski, Szymon, Marika Mróz, Agnieszka Bartoszek, and Barbara Kusznierewicz. "Post-Chromatographic Derivatization Coupled with Mass Spectrometry as a Method of Profiling and Identification of Antioxidants; Ligustrum vulgare Phytocomplex as an Example." Molecules 28, no. 24 (2023): 8000. http://dx.doi.org/10.3390/molecules28248000.

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High-performance liquid chromatography (HPLC) and high-performance thin-layer chromatography (HPTLC) coupled with radical scavenging assays, such as 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) can be both used for the detection of the antioxidants in plant extracts. In this study, the ethanolic (70% v/v) extracts from different morphological parts of Ligustrum vulgare collected at different stages of maturity were used as the source of antioxidants. The final identification of antioxidants was performed using high-resolution mass spectr

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Wójciak-Kosior, Magdalena, Agnieszka Skalska, Grażyna Matysik, and Magdalena Kryska. "Quantitative Analysis of Phenobarbital in Dosage Form by Thin-Layer Chromatography Combined with Densitometry." Journal of AOAC INTERNATIONAL 89, no. 4 (2006): 995–98. http://dx.doi.org/10.1093/jaoac/89.4.995.

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Abstract In this paper, a high-performance thin-layer chromatography (HPTLC) method combined with densitometry has been described. Chromatography was performed on silica gel Si 60F254 plates using dichloromethaneethyl acetateformic acid (9.5 + 0.5 + 0.1, v/v) mobile phase. This method has been successfully applied for the determination of phenobarbital in pharmaceuticals. Obtained results were comparable with traditionally used column high-performance liquid chromatography (HPLC) methods. For the proposed procedure, linearity (r &gt; 0.999), sensitivity (limit of detection 0.4 g/spot), rec

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Abdelrahman, Afnan E., Hadir M. Maher, and Nourah Z. Alzoman. "HPTLC Method for the Determination of Metformin Hydrochloride, Saxagliptin Hydrochloride, and Dapagliflozin in Pharmaceuticals." Current Analytical Chemistry 16, no. 5 (2020): 609–19. http://dx.doi.org/10.2174/1573407215666190131123029.

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Background: Type 2 diabetes mellitus is an expanding health problem. Binary antidiabetic combinations of Metformin Hydrochloride (MET) with either Saxagliptin Hydrochloride (SAX), or Dapagliflozin (DAP) are widely used. Review of the literature revealed that no single HPTLC method has been reported for the simultaneous determination of MET, SAX, and DAP allowing the determination of binary mixtures of any two of the three cited drugs in their tablets using the same experimental conditions, an important advantage for quality control. The advantages of HPTLC method relies on the simultaneous ana

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Jayaprakasam, R., M. F. Saleshier, and T. K. Ravi. "STANDARDIZATION OF HYGROPHILA SPINOSA AND ITS FORMULATION WITH REFERENCE TO LUPEOL, BY DEVELOPED AND VALIDATED HPTLC AND RP-HPLC METHODS." INDIAN DRUGS 52, no. 01 (2015): 13–19. http://dx.doi.org/10.53879/id.52.01.10182.

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The separation and determination of lupeol from Hygrophila spinosa were carried out by two simple, precise and accurate HPTLC and RP-HPLC methods. HPTLC method for the determination of lupeol from plant extract and its formulation was developed using a solvent system consisting of toluene : ethyl acetate : methanol (15: 3: 1.5%v/v/v). For detection, lupeol had to be derivatized with Liebermann Burchard reagent at 1050C. The optimum wavelength was fixed as 366nm. In RP-HPLC, the separation was carried out on a C18 column and the mobile phase selected was methanol: acetonitrile (30:70%v/v). The

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Yadav, Ramakant. "High Performance Thin-Layer Chromatography-Mass Spectrometry Evaluation of Sanguinarine and Dihydrosanguinarine from Argemone mexicana Seeds in Edible Mustard Oil." Bioscience Biotechnology Research Communications 15, no. 1 (2022): 158–63. http://dx.doi.org/10.21786/bbrc/15.1.24.

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In this present study, a simple, rapid, cheap, sensitive and reproducible HPTLC-MS Method has been developed for the identification of two important bioactive compounds, Sanguinarine and Dihydrosanguinarine in Argemone mexicana Linn seeds. The work further discussed and developed a sensitive HPTLC – MS method to analyse the adulteration and/or contamination of argemone oil in the edible mustard oil by spiking Sanguinarine and Dihydrosanguinarine as biomarkers. The n-hexane: diethyl ether (1:1 v/v) solvent system has been used as an extraction medium to extract the Sanguinarine and Dihydrosangu

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Chandrakar, Jagriti, Neeraj Agrawal, Lowkesh Chandravanshi, et al. "Quantitative Estimation of Piperine in Drakshadi Ghrita by HPTLC Method." Journal of Ravishankar University (PART-B) 38, no. 1 (2025): 143–50. https://doi.org/10.52228/jrub.2025-38-1-10.

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The present study focuses on the HPTLC estimation of piperine in Drakshadi Ghrita. The method involves the extraction of piperine from the Drakshadi Ghrita formulation, followed by its separation and quantification using an HPTLC system. The analysis is performed on a silica gel 60 F254 plate, using a suitable mobile phase for the optimal separation of piperine. The HPTLC analysis was performed using a developing solvent system consisting of toluene, ethyl acetate, and formic acid in a ratio of 7:3:0.3 (v/v/v). The HPTLC method accurately quantified piperine in Drakshadi Ghrita with Rf values

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Alam, Prawez, Faiyaz Shakeel, Mohammed H. Alqarni, Ahmed I. Foudah, Md Faiyazuddin, and Sultan Alshehri. "Rapid, Sensitive, and Sustainable Reversed-Phase HPTLC Method in Comparison to the Normal-Phase HPTLC for the Determination of Pterostilbene in Capsule Dosage Form." Processes 9, no. 8 (2021): 1305. http://dx.doi.org/10.3390/pr9081305.

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The greenness evaluation of literature analytical methods for pterostilbene (PT) analysis was not performed. Accordingly, the rapid, sensitive, and green/sustainable reversed-phase high-performance thin-layer chromatography (RP-HPTLC) method was developed and compared to the normal-phase (NP)-HPTLC (NP-HPTLC) for the estimation of PT with a classical univariate calibration. The RP quantification of PT was performed using green solvent systems; however, the NP analysis of PT was performed using routine solvent systems. The PT was detected at 302 nm for both of the methods. The greenness scores

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Foudah, Ahmed I., Faiyaz Shakeel, Mohammed H. Alqarni, Tariq M. Aljarba, Sultan Alshehri, and Prawez Alam. "Simultaneous Detection of Chlorzoxazone and Paracetamol Using a Greener Reverse-Phase HPTLC-UV Method." Separations 9, no. 10 (2022): 300. http://dx.doi.org/10.3390/separations9100300.

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In the literature, greener/eco-friendly analytical techniques for simultaneous estimation of chlorzoxazone (CZN) and paracetamol (PCT) are scarce. As a consequence, greener reverse-phase high-performance thin-layer chromatography with ultraviolet (HPTLC-UV) detection was developed and validated for simultaneous estimation of CZN and PCT in commercial capsules and tablets. The greenness of the proposed HPTLC-UV technique was assessed quantitatively by utilizing the “Analytical GREENness (AGREE)” methodology. For simultaneous estimation of CZN and PCT, the greener HPTLC-UV technique was linear i

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Khokhlova, KO. "QUALITY CONTROL OF HAWTHORN TINCTURE BY HPTLC METHOD." Annals of Mechnikov Institute, no. 3 (November 18, 2016): 56–60. https://doi.org/10.5281/zenodo.167436.

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Introduction. Hawthorn tincture is one of the most used herbal drugs at the domestic pharmaceutical market. According to the State register of drugs at the pharmaceutical market of Ukraine, there are 13 commercial offers of Hawthorn tincture from home-produced manufactures. The initial herbal raw materials for Hawthorn tincture are Hawthorn fruits, which are widespread at the territory of Ukraine. These are pharmacopoeial herbal raw material. Thus, 12 different species of Hawthorn fruits are included into monograph <Hawthorn fruits> of Ukrainian State Pharmacopoeia (SPhU) and State Pharm

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Guermouche, M. H., D. Habel, and S. Guermouche. "Assay of Tinidazole in Human Serum by High-Performance Thin-Layer Chromatography—Comparison with High-Performance Liquid Chromatography." Journal of AOAC INTERNATIONAL 82, no. 2 (1999): 244–47. http://dx.doi.org/10.1093/jaoac/82.2.244.

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Abstract Determination of tinidazole in human serum by high-performance thin-layer chromatography (HPTLC) is presented. It includes use of 10 × 10 cm plates coated with silica gel 60 and chloroform-acetonitrile-acetic acid (60 + 40 + 2) as mobile phase. Quantitation was performed by densitometry at 320 nm. The linearity (1-10 ng), precision (6%), reproducibility (5%), recovery (96%), and detection limit (1 mg/L) of tinidazole determination by HPTLC were comparable with corresponding method parameters by reversed-phase HPLC. A satisfactory correlation was found between the 2 analytical methods.

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Wani, G. P., and S. B. Jadhav. "RP-HPLC AND HPTLC STABILITY INDICATING ASSAY METHODS FOR IVERMECTIN IN BULK AND TABLET DOSAGE FORM." INDIAN DRUGS 55, no. 03 (2018): 32–42. http://dx.doi.org/10.53879/id.55.03.11143.

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Simple, rapid, precise, accurate RP-HPLC and HPTLC methods have been developed and validated for ivermectin in bulk and its marketed formulation. RP-HPLC method for drug was achieved on Grace C18 (250 mm X 4.6 ID, Particle size; 5 μ) column using mobile phase acetonitrile: 10 mM phosphate buffer (95:05 v/v) pH adjusted to 3 with o-phosphoric acid. Detection of drug was done at 245 nm. The retention time was found to be 5.83 min. HPTLC method for ivermectin was accomplished on a precoated silica gel aluminium plate 60F-254 (CAMAG Linomat 5), using toluene: methanol: glacial acetic acid (8:2:0.1

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Ibi, Afoke, Min Du, Till Beuerle, Dennis Melchert, Julia Solnier, and Chuck Chang. "A Multi-Pronged Technique for Identifying Equisetum palustre and Equisetum arvense—Combining HPTLC, HPLC-ESI-MS/MS and Optimized DNA Barcoding Techniques." Plants 11, no. 19 (2022): 2562. http://dx.doi.org/10.3390/plants11192562.

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The most prominent horsetail species, Equisetum arvense, has an array of different medicinal properties, thus the proper authentication and differentiation of the plant from the more toxic Equisetum palustre is important. This study sought to identify different samples of E. arvense and E. palustre using three analytical methods. The first method involved the use of HPTLC analysis, as proposed by the European Pharmacopoeia. The second, HPLC-ESI-MS/MS, is capable of both identification and quantification and was used to determine the Equisetum alkaloid content in each sample. A third method was

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