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Journal articles on the topic 'HS28'

Author: Grafiati

Published: 4 June 2021

Last updated: 2 February 2022

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1

Rodrigues, Fabricio, Renzo Garcia Von Pinho, Carlos Juliano Brant Albuquerque, and Édila Vilela Resende Von Pinho. "Índice de seleção e estimativa de parâmetros genéticos e fenotípicos para características relacionadas com a produção de milho-verde." Ciência e Agrotecnologia 35, no. 2 (2011): 278–86. http://dx.doi.org/10.1590/s1413-70542011000200007.

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O Brasil é um dos maiores produtores de milho do mundo e possui grande potencial para a produção de milho verde. Além de agregar valor ao produto, a comercialização de milho verde cresceu muito nos últimos anos. Há escassez de informações sobre a avaliação e a obtenção de cultivares destinadas à produção de milho-verde e também sobre estudos genéticos das características envolvidas nessa produção. Objetivou-se, neste trabalho, estimar parâmetros genéticos e fenotípicos, a correlação genética, classificar e identificar híbridos de milho verde promissores ao mercado consumidor e avaliar a eficiê

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2

Morley, B. J., C. A. Abbott, J. A. Sharpe, J. Lida, P. S. Chan-Thomas, and W. G. Wood. "A single beta-globin locus control region element (5' hypersensitive site 2) is sufficient for developmental regulation of human globin genes in transgenic mice." Molecular and Cellular Biology 12, no. 5 (1992): 2057–66. http://dx.doi.org/10.1128/mcb.12.5.2057.

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The beta-globin gene complex is regulated by an upstream locus control region (LCR) which is responsible for high-level, position-independent, erythroid-cell-specific expression of the genes in the cluster. Its role in the developmental regulation of beta-like globin gene transcription remains to be established. We have examined the effect of a single LCR element, hypersensitive site 2 (HS2), on the developmental regulation of the human fetal gamma and adult beta genes in transgenic mice. In mice bearing HS2A gamma beta and HS2G gamma A gamma-117 delta beta human globin gene constructs, switch

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3

Morley, B. J., C. A. Abbott, J. A. Sharpe, J. Lida, P. S. Chan-Thomas, and W. G. Wood. "A single beta-globin locus control region element (5' hypersensitive site 2) is sufficient for developmental regulation of human globin genes in transgenic mice." Molecular and Cellular Biology 12, no. 5 (1992): 2057–66. http://dx.doi.org/10.1128/mcb.12.5.2057-2066.1992.

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The beta-globin gene complex is regulated by an upstream locus control region (LCR) which is responsible for high-level, position-independent, erythroid-cell-specific expression of the genes in the cluster. Its role in the developmental regulation of beta-like globin gene transcription remains to be established. We have examined the effect of a single LCR element, hypersensitive site 2 (HS2), on the developmental regulation of the human fetal gamma and adult beta genes in transgenic mice. In mice bearing HS2A gamma beta and HS2G gamma A gamma-117 delta beta human globin gene constructs, switch

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4

Cordaillat, Magali, Caroline Rugale, Daniel Casellas, Albert Mimran, and Bernard Jover. "Cardiorenal abnormalities associated with high sodium intake: correction by spironolactone in rats." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 289, no. 4 (2005): R1137—R1143. http://dx.doi.org/10.1152/ajpregu.00154.2005.

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Reversal by the mineralocorticoid receptor antagonist spironolactone on cardiac and renal abnormalities, associated with long-term (since weaning) administration of a high (2 and 8% NaCl chow, HS2 and HS8) sodium diet, was assessed in Sprague-Dawley rats. At the age of 5 mo, spironolactone (20 or 100 mg/kg, gavage) or placebo were given for 14 days to HS2 and HS8 rats. A group fed a regular diet (0.8% NaCl, NS) remained untreated. High sodium intake had no detectable effect on blood pressure; however, cardiac mass index and cross-sectional area of the carotid artery, as well as albuminuria, we

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5

Rodrigues, Fabricio, Renzo Garcia Von Pinho, Carlos Juliano Brant Albuquerque, Edmir Marques Faria Filho, and Jeanne de Cássia Goulart. "Capacidade de combinação entre linhagens de milho visando à produção de milho verde." Bragantia 68, no. 1 (2009): 75–84. http://dx.doi.org/10.1590/s0006-87052009000100009.

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No Brasil, há escassez de informações sobre a avaliação e obtenção de cultivares destinadas à produção de milho verde, bem como estudos genéticos de características envolvidas nesta produção. Deste modo, este trabalho teve por objetivo determinar o tipo de ação gênica predominante no controle das características e estimar a capacidade geral de combinação (CGC) e a capacidade específica de combinação (CEC) entre linhagens endogâmicas de milho visando à produção de milho verde. As oito linhagens e os 28 híbridos foram avaliados no ano agrícola de 2004/2005, no município de Lavras e em Ijaci, amb

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6

Decker, Brian K., Nigel G. Adams, and Lucia M. Babcock. "Gas-phase reactivity of HS2H+· and S2+·: an investigation of the gas basicity and proton affinity of HS2·." International Journal of Mass Spectrometry 185-187 (April 1999): 727–43. http://dx.doi.org/10.1016/s1387-3806(98)14175-1.

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7

Eissenberg, Joel C., and Sarah C. R. Elgin. "Hsp28stl: A P-Element Insertion Mutation That Alters the Expression of a Heat Shock Gene in Drosophila melanogaster." Genetics 115, no. 2 (1987): 333–40. http://dx.doi.org/10.1093/genetics/115.2.333.

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ABSTRACT We have identified and cloned a mutant allele of the small heat shock gene Hsp28 of Drosophila melanogaster. This allele, which we have called Hsp28stl, produces small amounts of a single aberrantly large, heat-inducible transcript in heat-shocked flies, while a normal-sized Hsp28 transcript is present only in fertile females. No Hsp28 transcript at all is detected in mutant prepupae, a stage when wild-type flies show high levels of Hsp28 RNA. We have cloned the Hsp28stl allele, and have found that a 1.3-kb defective P-element is present 5' to Hsp28 in the mutant line. The site of P-e

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8

Miao, Shiwei, Li Fan, Liang Zhao, et al. "Physicochemical and Biological Characterization of the Proposed Biosimilar Tocilizumab." BioMed Research International 2017 (2017): 1–13. http://dx.doi.org/10.1155/2017/4926168.

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HS628 has been developed as a proposed biosimilar product of originator tocilizumab (Actemra®). An extensive physicochemical and biological characterization was conducted to assess similarity between HS628 and originator tocilizumab. The amino acid sequence was shown to be identical between HS628 and originator tocilizumab. The higher order structure was found to be indistinguishable from originator tocilizumab. Concerning purity and heterogeneity, HS628 was demonstrated to have similar posttranslational modifications, charge heterogeneity, size heterogeneity, and glycosylation to originator t

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9

NAGATA, HIRONOBU. "HS20 Type Sizing Machine." Sen'i Gakkaishi 48, no. 5 (1992): P208—P210. http://dx.doi.org/10.2115/fiber.48.5_p208.

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10

Huang, Wenxiang, Xingyu Liu, Xiaosi Zhou, Xiaoli Wang, Xinyu Liu, and Hongxia Liu. "Calcium Signaling Is Suppressed in Magnaporthe oryzae Conidia by Bacillus cereus HS24." Phytopathology® 110, no. 2 (2020): 309–16. http://dx.doi.org/10.1094/phyto-08-18-0311-r.

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Rice yield is greatly reduced owing to rice blast, a polycyclic fungal disease caused by the ascomycete Magnaporthe oryzae. Previously, Bacillus cereus HS24, isolated from a rice farm, showed a strong antimicrobial effect toward M. oryzae. To better exploit it as a biocontrol agent, HS24 was studied for the mechanism that it uses to suppress rice blast. Conidium germination in M. oryzae was significantly inhibited by HS24, whereby inhibition reached 97.8% at the concentration of 107 CFU/ml. The transcription levels of Ca2+/calmodulin-dependent protein kinase II, PMC1, and CCH1, key genes invol

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11

Arrigo, A. P. "The monovalent ionophore monensin maintains the nuclear localization of the human stress protein hsp28 during heat shock recovery." Journal of Cell Science 96, no. 3 (1990): 419–27. http://dx.doi.org/10.1242/jcs.96.3.419.

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In HeLa cells exposed to supra-optimal temperatures, the alpha-crystallin-related stress protein hsp28 is reversibly redistributed inside the nucleus and increases its level of phosphorylation and aggregation. Here, I show that, at normal temperature after a heat stress, the sodium ionophore monensin maintains the nuclear localization of hsp28 without impairing the dephosphorylation of this protein. This phenomenon is not due to a prolongation, by monensin, of the synthesis of the heat-shock proteins after the heat stress. In contrast, the potassium ionophore nonactin induces only a weak alter

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12

Rogers, David. "Why build HS2?" Construction Research and Innovation 5, no. 2 (2014): 18–23. http://dx.doi.org/10.1080/20450249.2014.11873930.

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13

Thawai, Chitti, Wasinee Thamsathit, and Takuji Kudo. "Planosporangium thailandense sp. nov., isolated from soil from a Thai hot spring." International Journal of Systematic and Evolutionary Microbiology 63, Pt_3 (2013): 1051–55. http://dx.doi.org/10.1099/ijs.0.043539-0.

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A novel filamentous bacterial strain, HSS8-18T, was isolated from soil from a hot-spring pond in Krabi province, Thailand. The isolate produced single globose bodies and short, finger-like sporangia directly from the substrate mycelium. The cell wall of the isolate contained meso-diaminopimelic acid, glycine, arabinose and xylose. The characteristic phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, phosphatidylethanolamine and hydroxyl phosphatidylethanolamine. The predominant menaquinones were MK-10(H2), MK-9(H4) and MK-10(

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14

Arrigo, A. P. "Tumor necrosis factor induces the rapid phosphorylation of the mammalian heat shock protein hsp28." Molecular and Cellular Biology 10, no. 3 (1990): 1276–80. http://dx.doi.org/10.1128/mcb.10.3.1276.

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Tumor necrosis factor alpha was found to rapidly phosphorylate the unique mammalian small heat shock protein hsp28 without impairing its cytoplasmic localization and without inducing the synthesis of the heat shock proteins. In contrast to the C-kinase-dependent phosphorylation of hsp28 in response to the tumor promoter phorbol-12-myristate-13-acetate, the heat- and tumor necrosis factor-mediated phosphorylation of this heat shock protein appears to occur independently of C kinase. These observations suggest that a C-kinase-independent phosphorylation of hsp28 may be an early event in the cell

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15

Arrigo, A. P. "Tumor necrosis factor induces the rapid phosphorylation of the mammalian heat shock protein hsp28." Molecular and Cellular Biology 10, no. 3 (1990): 1276–80. http://dx.doi.org/10.1128/mcb.10.3.1276-1280.1990.

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Tumor necrosis factor alpha was found to rapidly phosphorylate the unique mammalian small heat shock protein hsp28 without impairing its cytoplasmic localization and without inducing the synthesis of the heat shock proteins. In contrast to the C-kinase-dependent phosphorylation of hsp28 in response to the tumor promoter phorbol-12-myristate-13-acetate, the heat- and tumor necrosis factor-mediated phosphorylation of this heat shock protein appears to occur independently of C kinase. These observations suggest that a C-kinase-independent phosphorylation of hsp28 may be an early event in the cell

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16

Chang, Yuan-Jhe, Ya-Chun Chang, Rosa Huang Liu, Chia-Wen Chen, Inn Lee, and Nae-Cherng Yang. "Resveratrol Can Be Stable in a Medium Containing Fetal Bovine Serum with Pyruvate but Shortens the Lifespan of Human Fibroblastic Hs68 Cells." Oxidative Medicine and Cellular Longevity 2018 (2018): 1–15. http://dx.doi.org/10.1155/2018/2371734.

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This study is aimed at developing a method that can inhibit resveratrol (Res) degradation in Dulbecco’s modified Eagle medium (DMEM) and at evaluating the effects of Res on the replicative lifespan of Hs68 cells. We hypothesized that Res can extend the lifespan of Hs68 cells if we can inhibit the oxidative degradation of Res in the medium. We found that the addition of ≥5 U/mL SOD to the medium could completely inhibit Res degradation in DMEM. Fetal bovine serum (FBS) contained 29.3 ± 1.1 U/mL of SOD activity. FBS could prevent Res degradation in the medium through SOD activity and Res–FBS int

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17

Sakai, Akira, Miyo Oda, Noriaki Yoshida, Mitsuhiro Itagaki, Koji Arihiro, and Akiro Kimura. "Establishment of An HS23 Stromal Cell-Dependent Myeloma Cell Line: Fibronectin and IL-6 Are Critical for Survival." Blood 116, no. 21 (2010): 2949. http://dx.doi.org/10.1182/blood.v116.21.2949.2949.

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Abstract Abstract 2949 Adhesion of multiple myeloma (MM) cells to bone marrow stromal cells triggers cytokine-mediated tumor cell growth, survival, and drug resistance. In particular, integrin a4b1, very late antigen 4 (VLA-4)-mediated fibronectin adhesion confers a survival advantage to myeloma cells. One of the problems in treating patients with MM is that it is very hard to eliminate residual myeloma cells, even following high-dose chemotherapy followed by auto-stem cell transplantation. Importantly, cell adhesion-mediated drug resistance (CAM-DR) must be overcome in order to eliminate the

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18

Kim, Ha-Rim, Sol Kim, Eun Yee Jie, et al. "Effects of Tiarella polyphylla D. Don Callus Extract on Photoaging in Human Foreskin Fibroblasts Hs68 Cells." Natural Product Communications 16, no. 5 (2021): 1934578X2110169. http://dx.doi.org/10.1177/1934578x211016970.

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Ultraviolet (UV)-irradiation causes an overproduction of matrix metalloproteinases (MMPs) and collagen (COL) degradation causing skin sunburn, inflammation, or photoaging in human. In this study, we prepared callus from Tiarella polyphylla D. Don ( T. polyphylla) stem and its phytochemical profiles were analyzed using HPLC-MWD. The effects of T. polyphylla callus extract evaluated against on UVB-induced damage in human foreskin fibroblast (Hs68). Hs68 was exposed to UVB in the presence or absence of T. polyphylla callus extract at concentrations of 100 and 250 µg/mL. Cell damage caused by UVB

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19

Kong, S., D. Bohl, C. Li, and D. Tuan. "Transcription of the HS2 enhancer toward a cis-linked gene is independent of the orientation, position, and distance of the enhancer relative to the gene." Molecular and Cellular Biology 17, no. 7 (1997): 3955–65. http://dx.doi.org/10.1128/mcb.17.7.3955.

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The locus control region (LCR) regulates transcription of the downstream beta-like globin genes 10 to 50 kb away. Among hypersensitive sites HS4, -3, -2, and -1, which define the LCR in erythroid cells, HS2 possesses prominent enhancer function. The mechanism by which the HS2 enhancer and other functional components of the LCR act over the distance is not clear. We have used reverse transcription-PCR and RNase protection assays to analyze the transcriptional statuses of both the endogenous and the transfected HS2 enhancer in erythroid K562 cells. A novel pattern of HS2 enhancer transcription w

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20

Alnuqaydan, Abdullah M. "Tamarix articulata Extracts Exhibit Antioxidant Activity and Offer Protection against Hydrogen Peroxide-Mediated Toxicity to Human Skin Fibroblasts." Journal of Toxicology 2020 (November 19, 2020): 1–11. http://dx.doi.org/10.1155/2020/8896263.

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Tamarix articulata (TA) is a wild halophytic plant growing in extremely harsh environmental conditions in the deserts of Saudi Arabia. Evaluating the protective effect of the methanolic extract of different parts (fresh and dry leaves, stem, and root) of TA was determined by MTT assay using Hs27 skin fibroblasts as the cellular model. The study was designed and conducted in two sets. The first set assesses the toxicity profile of TA extracts in both concentration- and time-dependent ways on Hs27 cells. Our MTT results showed that methanolic extracts from all four parts of TA at varying doses (

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21

Park, Ji-Eun, Hyoung Ja Kim, Su-Nam Kim, Seung Hyun Kang, and Youn Joon Kim. "Inhibitory Effect of Rosa multiflora hip Extract on UVB-induced Skin Photoaging in Hs68 Fibroblasts." Journal of the Society of Cosmetic Scientists of Korea 41, no. 4 (2015): 351–59. http://dx.doi.org/10.15230/scsk.2015.41.4.351.

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22

McDowell, Jennifer C., та Ann Dean. "Structural and Functional Cross-Talk between a Distant Enhancer and the ɛ-Globin Gene Promoter Shows Interdependence of the Two Elements in Chromatin". Molecular and Cellular Biology 19, № 11 (1999): 7600–7609. http://dx.doi.org/10.1128/mcb.19.11.7600.

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ABSTRACT We investigated the requirements for enhancer-promoter communication by using the human β-globin locus control region (LCR) DNase I-hypersensitive site 2 (HS2) enhancer and the ɛ-globin gene in chromatinized minichromosomes in erythroid cells. Activation of globin genes during development is accompanied by localized alterations of chromatin structure, and CACCC binding factors and GATA-1, which interact with both globin promoters and the LCR, are believed to be critical for globin gene transcription activation. We found that an HS2 element mutated in its GATA motif failed to remodel t

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23

Yang, Nae-Cherng, Yu-Hung Cho, and Inn Lee. "The Lifespan Extension Ability of Nicotinic Acid Depends on Whether the Intracellular NAD+ Level Is Lower than the Sirtuin-Saturating Concentrations." International Journal of Molecular Sciences 21, no. 1 (2019): 142. http://dx.doi.org/10.3390/ijms21010142.

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Calorie restriction can extend lifespan by increasing intracellular nicotinamide adenine dinucleotide (NAD+), thereby upregulating the activity of sirtuins (Caenorhabditis elegans Sir-2.1; human SIRT1). Nicotinic acid (NA) can be metabolized to NAD+; however, the calorie restriction mimetic (CRM) potential of NA is unclear. This study explored the ability and mechanism of NA to extend the lifespan of human Hs68 cells and C. elegans. We found that NA can efficiently increase the intracellular NAD+ levels in Hs68 cells and C. elegans; however, NA was only able to extend the lifespan of C. elegan

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24

Teoh, L. C. "HS08 CONGENITAL HAND: THE DEFORMITY-DEFICIENCY INDEX." ANZ Journal of Surgery 77, s1 (2007): A35. http://dx.doi.org/10.1111/j.1445-2197.2007.04120_8.x.

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25

Anderson, K. P., J. A. Lloyd, E. Ponce, S. C. Crable, J. C. Neumann, and J. B. Lingrel. "Regulated expression of the human beta globin gene in transgenic mice requires an upstream globin or nonglobin promoter." Molecular Biology of the Cell 4, no. 10 (1993): 1077–85. http://dx.doi.org/10.1091/mbc.4.10.1077.

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Transgenic mice have been used extensively to study elements governing the erythroid-specific developmental switch from human fetal gamma to human adult beta globin. Previous work demonstrated that a small construct composed of hypersensitive site 2 (HS2) of the locus control region (LCR) linked to the gamma and beta globin genes (HS2-gamma-beta) is sufficient for correct tissue and temporal expression of these genes, whereas HS2-beta alone is inappropriately expressed in the embryo. Two models, which are not mutually exclusive, have been proposed to explain these results and those of other co

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26

Cornelussen, Richard N. M., Ger J. Van Der Vusse, Theo H. M. Roemen, and Luc H. E. H. Snoeckx. "Heat pretreatment differentially affects cardiac fatty acid accumulation during ischemia and postischemic reperfusion." American Journal of Physiology-Heart and Circulatory Physiology 280, no. 4 (2001): H1736—H1743. http://dx.doi.org/10.1152/ajpheart.2001.280.4.h1736.

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We investigated whether the cardioprotection induced by heat stress (HS) pretreatment is associated with mitigation of phospholipid degradation during the ischemic and/or postischemic period. The hearts, isolated from control rats and from heat-pretreated rats (42°C for 15 min) either 30 min (HS0.5-h) or 24 h (HS24-h) earlier, were subjected to 45 min of no-flow ischemia, followed by 45 min of reperfusion. Unesterified arachidonic acid (AA) accumulation was taken as a measure for phospholipid degradation. Significantly improved postischemic ventricular functional recovery was only found in the

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27

Erni, Dominique, Reto Wettstein, Sören Schramm, et al. "Normovolemic hemodilution with Hb vesicle solution attenuates hypoxia in ischemic hamster flap tissue." American Journal of Physiology-Heart and Circulatory Physiology 284, no. 5 (2003): H1702—H1709. http://dx.doi.org/10.1152/ajpheart.00821.2002.

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The aim of this study was to test whether oxygenation in acutely ischemic, collateralized tissue may be improved by normovolemic hemodilution with a solution containing liposome-encapsulated human Hb (HbV). A skin flap model in anesthetized hamsters was used, which consisted of two parts receiving either anatomic or collateral perfusion. Microhemodynamics were investigated with intravital microscopy. Partial tissue oxygen tension was measured with a Clark-type microprobe. Hemodilution was obtained by exchanging 50% of the total blood volume with HbV suspended in 8% human serum albumin (HSA8) o

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28

Sweet, Rod. "HS2’s moment of truth." Construction Research and Innovation 10, no. 3 (2019): 54–59. http://dx.doi.org/10.1080/20450249.2019.1654235.

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29

Miller, JL, CE Walsh, PA Ney, RJ Samulski, and AW Nienhuis. "Single-copy transduction and expression of human gamma-globin in K562 erythroleukemia cells using recombinant adeno-associated virus vectors: the effect of mutations in NF-E2 and GATA-1 binding motifs within the hypersensitivity site 2 enhancer [published erratum appears in Blood 1995 Feb 1;85(3):862]." Blood 82, no. 6 (1993): 1900–1906. http://dx.doi.org/10.1182/blood.v82.6.1900.1900.

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Abstract The use of recombinant adeno-associated virus (rAAV) vectors provides a new strategy to investigate the role of specific regulatory elements and trans-acting factors in globin gene expression. We linked hypersensitivity site 2 (HS2) from the locus control region (LCR) to a A gamma-globin gene (A gamma*) mutationally marked to allow its transcript to be distinguished from endogenous gamma-globin mRNA. The vector also contains the phosphotransferase gene that confers resistance to neomycin (NeoR). HS2 region mutations within the NF-E2 motifs prevented NF-E2 binding while preserving AP-1

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30

Miller, JL, CE Walsh, PA Ney, RJ Samulski, and AW Nienhuis. "Single-copy transduction and expression of human gamma-globin in K562 erythroleukemia cells using recombinant adeno-associated virus vectors: the effect of mutations in NF-E2 and GATA-1 binding motifs within the hypersensitivity site 2 enhancer [published erratum appears in Blood 1995 Feb 1;85(3):862]." Blood 82, no. 6 (1993): 1900–1906. http://dx.doi.org/10.1182/blood.v82.6.1900.bloodjournal8261900.

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The use of recombinant adeno-associated virus (rAAV) vectors provides a new strategy to investigate the role of specific regulatory elements and trans-acting factors in globin gene expression. We linked hypersensitivity site 2 (HS2) from the locus control region (LCR) to a A gamma-globin gene (A gamma*) mutationally marked to allow its transcript to be distinguished from endogenous gamma-globin mRNA. The vector also contains the phosphotransferase gene that confers resistance to neomycin (NeoR). HS2 region mutations within the NF-E2 motifs prevented NF-E2 binding while preserving AP-1 binding.

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31

Grathwohl, P., and K. U. Totsche. "Symposium no HSA8: Natural attenuation and intrinsic bioremediation." Physics and Chemistry of the Earth, Part B: Hydrology, Oceans and Atmosphere 24, no. 6 (1999): 493. http://dx.doi.org/10.1016/s1464-1909(99)00035-0.

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32

Li, Qiao, Ting Zou, Peng Ai, et al. "Complete genome sequence of Bacillus thuringiensis HS18-1." Journal of Biotechnology 214 (November 2015): 61–62. http://dx.doi.org/10.1016/j.jbiotec.2015.08.017.

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33

Bungert, Jörg, Keiji Tanimoto, Sunil Patel, Qinghui Liu, Mark Fear та James Douglas Engel. "Hypersensitive Site 2 Specifies a Unique Function within the Human β-Globin Locus Control Region To Stimulate Globin Gene Transcription". Molecular and Cellular Biology 19, № 4 (1999): 3062–72. http://dx.doi.org/10.1128/mcb.19.4.3062.

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ABSTRACT The human β-globin locus control region (LCR) harbors both strong chromatin opening and enhancer activity when assayed in transgenic mice. To understand the contribution of individual DNase I hypersensitive sites (HS) to the function of the human β-globin LCR, we have mutated the core elements within the context of a yeast artificial chromosome (YAC) carrying the entire locus and then analyzed the effect of these mutations on the formation of LCR HS elements and expression of the genes in transgenic mice. In the present study, we examined the consequences of two different HS2 mutation

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34

Fan, Chao, Fazhe Sun, Xiaomei Wang, et al. "Synthesis of ZnO Hierarchical Structures and Their Gas Sensing Properties." Nanomaterials 9, no. 9 (2019): 1277. http://dx.doi.org/10.3390/nano9091277.

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Firecracker-like ZnO hierarchical structures (ZnO HS1) were synthesized by combining electrospinning with hydrothermal methods. Flower-like ZnO hierarchical structures (ZnO HS2) were prepared by a hydrothermal method using ultrasound-treated ZnO nanofibers (ZnO NFs) as raw material which has rarely been reported in previous papers. Scanning electron microscope (SEM) and transmission electron microscope’s (TEM) images clearly indicated the existence of nanoparticles on the ZnO HS2 material. Both gas sensors exhibited high selectivity toward H2S gas over various other gases at 180 °C. The ZnO HS

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35

Miley, Michael J., Ilhem Messaoudi, Beatrix M. Metzner, Yudong Wu, Janko Nikolich-Žugich, and Daved H. Fremont. "Structural Basis for the Restoration of TCR Recognition of an MHC Allelic Variant by Peptide Secondary Anchor Substitution." Journal of Experimental Medicine 200, no. 11 (2004): 1445–54. http://dx.doi.org/10.1084/jem.20040217.

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Major histocompatibility complex (MHC) class I variants H-2Kb and H-2Kbm8 differ primarily in the B pocket of the peptide-binding groove, which serves to sequester the P2 secondary anchor residue. This polymorphism determines resistance to lethal herpes simplex virus (HSV-1) infection by modulating T cell responses to the immunodominant glycoprotein B498-505 epitope, HSV8. We studied the molecular basis of these effects and confirmed that T cell receptors raised against Kb–HSV8 cannot recognize H-2Kbm8–HSV8. However, substitution of SerP2 to GluP2 (peptide H2E) reversed T cell receptor (TCR) r

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36

Langdon, Scott D., and Russel E. Kaufman. "Gamma-Globin Gene Promoter Elements Required for Interaction With Globin Enhancers." Blood 91, no. 1 (1998): 309–18. http://dx.doi.org/10.1182/blood.v91.1.309.

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Abstract Normal expression of the human β-globin domain genes is dependent on at least three types of regulatory elements located within the β-globin domain: the locus control region (LCR), globin enhancer elements (3′β and 3′Aγ), and the individual globin gene promoter and upstream regions. It has been postulated that regulation occurs through physical interactions between factors bound to these elements, which are located at considerable distances from each other. To identify the elements required for promoter-enhancer interactions from a distance, we have investigated the expression of the

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Langdon, Scott D., and Russel E. Kaufman. "Gamma-Globin Gene Promoter Elements Required for Interaction With Globin Enhancers." Blood 91, no. 1 (1998): 309–18. http://dx.doi.org/10.1182/blood.v91.1.309.309_309_318.

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Normal expression of the human β-globin domain genes is dependent on at least three types of regulatory elements located within the β-globin domain: the locus control region (LCR), globin enhancer elements (3′β and 3′Aγ), and the individual globin gene promoter and upstream regions. It has been postulated that regulation occurs through physical interactions between factors bound to these elements, which are located at considerable distances from each other. To identify the elements required for promoter-enhancer interactions from a distance, we have investigated the expression of the wild-type

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Wang, Bin, Yang Ji, Yonghua Xia, Kunhao Qin, and Bo Li. "The exploitation of thermophile resources in hot springs: fluorescent carbon dots derived from Ureibacillus thermosphaericus for multicolour cellular imaging and selectivity detection of heavy metals." Analytical Methods 13, no. 15 (2021): 1810–15. http://dx.doi.org/10.1039/d0ay02213f.

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Poma, Anna, Giulia Vecchiotti, Sabrina Colafarina, et al. "In Vitro Genotoxicity of Polystyrene Nanoparticles on the Human Fibroblast Hs27 Cell Line." Nanomaterials 9, no. 9 (2019): 1299. http://dx.doi.org/10.3390/nano9091299.

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Several studies have provided information on environmental nanoplastic particles/debris, but the in vitro cyto-genotoxicity is still insufficiently characterized. The aim of this study is to analyze the effects of polystyrene nanoparticles (PNPs) in the Hs27 cell line. The viability of Hs27 cells was determined following exposure at different time windows and PNP concentrations. The genotoxic effects of the PNPs were evaluated by the cytokinesis-block micronucleus (CBMN) assay after exposure to PNPs. We performed ROS analysis on HS27 cells to detect reactive oxygen species at different times a

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Nagata, Fusaomi, and Keigo Watanabe. "Neural Network-Based Inverse Kinematics for Motoman HS20 and Its Efficient Learning Method." Journal of the Institute of Industrial Applications Engineers 4, no. 4 (2016): 166–71. http://dx.doi.org/10.12792/jiiae.4.166.

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Cannas, M., M. R. Amedeo, G. Capello, F. Denicolai, and G. Lorenzetti. "Biocompatibility of an HS21 Stellite in relation to fibronectin." Journal of Biomechanics 20, no. 8 (1987): 824. http://dx.doi.org/10.1016/0021-9290(87)90132-1.

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Tuan, Dorothy, Xingguo Zhu, Jianhua Ling, Ling Zhang, Wenhu Pi та Min Wu. "A Facilitated Tracking and Transcription Mechanism of the HS2 Enhancer in the β-Globin Locus Control Region." Blood 108, № 11 (2006): 1596. http://dx.doi.org/10.1182/blood.v108.11.1596.1596.

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Abstract The 100 kb human β-globin gene locus contains the embryonic ε-, the fetal Gγ- and Aγ-, and the adult δ- and β-globin genes arranged in the transcriptional order of 5′ ε-Gγ-Aγ-δ-β 3′. The β-globin Locus Control Region (LCR), defined by DNase I hypersensitive sites HS1, 2, 3, 4 and 5 located far upstream of the globin genes, is absolutely required for transcriptional activation of the globin genes in erythroid cells. The HS2 site, located respectively 11 and 55 kb upstream of the ε- and β-globin genes, possesses prominent enhancer activity and is able to activate transcription of the gl

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Cimbora, Daniel M., Dirk Schübeler, Andreas Reik та ін. "Long-Distance Control of Origin Choice and Replication Timing in the Human β-Globin Locus Are Independent of the Locus Control Region". Molecular and Cellular Biology 20, № 15 (2000): 5581–91. http://dx.doi.org/10.1128/mcb.20.15.5581-5591.2000.

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ABSTRACT DNA replication in the human β-globin locus is subject to long-distance regulation. In murine and human erythroid cells, the human locus replicates in early S phase from a bidirectional origin located near the β-globin gene. This Hispanic thalassemia deletion removes regulatory sequences located over 52 kb from the origin, resulting in replication of the locus from a different origin, a shift in replication timing to late S phase, adoption of a closed chromatin conformation, and silencing of globin gene expression in murine erythroid cells. The sequences deleted include nuclease-hyper

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Forsberg, E. Camilla, Karen M. Downs та Emery H. Bresnick. "Direct interaction of NF-E2 with hypersensitive site 2 of the β-globin locus control region in living cells". Blood 96, № 1 (2000): 334–39. http://dx.doi.org/10.1182/blood.v96.1.334.

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Abstract The human β-globin locus control region (LCR) confers high-level, tissue-specific expression to the β-globin genes. Tandem Maf recognition elements (MAREs) within the hypersensitive site 2 (HS2) subregion of the LCR are important for the strong enhancer activity of the LCR. Multiple proteins are capable of interacting with these sites in vitro, including the erythroid cell- and megakaryocyte-specific transcription factor, NF-E2. The importance of NF-E2 for β-globin gene expression is evident in murine erythroleukemia cells lacking the p45 subunit of NF-E2. These CB3 cells have a sever

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Forsberg, E. Camilla, Karen M. Downs та Emery H. Bresnick. "Direct interaction of NF-E2 with hypersensitive site 2 of the β-globin locus control region in living cells". Blood 96, № 1 (2000): 334–39. http://dx.doi.org/10.1182/blood.v96.1.334.013k17_334_339.

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The human β-globin locus control region (LCR) confers high-level, tissue-specific expression to the β-globin genes. Tandem Maf recognition elements (MAREs) within the hypersensitive site 2 (HS2) subregion of the LCR are important for the strong enhancer activity of the LCR. Multiple proteins are capable of interacting with these sites in vitro, including the erythroid cell- and megakaryocyte-specific transcription factor, NF-E2. The importance of NF-E2 for β-globin gene expression is evident in murine erythroleukemia cells lacking the p45 subunit of NF-E2. These CB3 cells have a severe defect

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Hlavacs, George M., Todd Long, Richard A. Miller, and T. Micheal Baseheart. "Nondestructive Determination of Response of Shear Keys to Environmental and Structural Cyclic Loading." Transportation Research Record: Journal of the Transportation Research Board 1574, no. 1 (1997): 18–24. http://dx.doi.org/10.3141/1574-03.

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Adjacent box girder bridges use grouted shear keys to share loads among beams. These keys tend to crack and leak. A full-scale portion of an adjacent box girder bridge is being used to test the performance of grouted shear keys under environmental and cyclic loads. The pulse velocity method is employed to find cracks in the shear keys. Two separate tests were conducted. In the first, shear keys that were grouted in late autumn cracked soon after casting, before any load had been applied. Data from instruments embedded in the beams and shear keys showed large discontinuities in strain caused by

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Tsay, Gregory J., Shin-Yi Lin, Chien-Yu Li, Jeng-Leun Mau, and Shu-Yao Tsai. "Comparison of Single and Combined Use of Ergothioneine, Ferulic Acid, and Glutathione as Antioxidants for the Prevention of Ultraviolet B Radiation-Induced Photoaging Damage in Human Skin Fibroblasts." Processes 9, no. 7 (2021): 1204. http://dx.doi.org/10.3390/pr9071204.

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Ultraviolet B (UVB) irradiation can cause human skin damage or skin aging and wrinkle formation through photochemical reactions. Antioxidative substances may ameliorate UV damage. In this study, the anti-photoaging activity of three antioxidants—ergothioneine, ferulic acid, and glutathione—was investigated after UVB irradiation of Hs68 human skin fibroblast cells. The cells treated with these three antioxidants appeared similar to unirradiated control cells. UVB irradiation decreased cell viability by 26% compared to that of unirradiated control cells. However, the addition of either single or

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Gibson, J. R., C. Fitzgerald, and R. J. Owen. "Comparison of PFGE, ribotyping and phage-typing in the epidemiological analysis ofCampylobacter jejuniserotype HS2 infections." Epidemiology and Infection 115, no. 2 (1995): 215–25. http://dx.doi.org/10.1017/s0950268800058349.

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SummaryIn this study we have evaluated the ability of three typing methods, pulsed field gel electrophoresis (PFGE), phage-typing and ribotyping, to discriminate not only between strains of differing serotypes but also between strains within a single serotype, heat stable serotype 2 (HS2). Forty-five isolates derived from cases of campylobacter enteritis occurring in the Cardiff area were examined. These included 18, mostly HS2, strains associated with an outbreak. The typing results for these and a further 39 epidemiologically unrelated strains of serotype HS2 were compared. This is the first

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Roderburg, Christoph, Anke Diehlmann, Frederik Wein, et al. "The Hematopoietic Supportive Potential of Human Mesenchymal Stromal Cells Is Associated with Expression of Cadherins." Blood 108, no. 11 (2006): 1402. http://dx.doi.org/10.1182/blood.v108.11.1402.1402.

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Abstract Self renewal and differentiation of hematopoietic stem cells (HSC) are governed by interaction with the supportive microenvironment of the bone marrow. Secreted factors as well as specific cell adhesion proteins are involved in this interaction. As an in vitro model system, the hematopoietic microenvironment can be mimicked by supportive mesenchymal stromal cells (MSC). We have compared the supportive potential of human MSC from bone marrow (BM) isolated under two different culture conditions (BM-MSC-M1 and BM-MSC-M2), from adipose tissue (AT) and umbilical cord blood (CB) that were a

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Clarke, Tegan N., Megan A. Schilling, Luca A. Melendez, Sandra D. Isidean, Chad K. Porter, and Frédéric M. Poly. "A systematic review and meta-analysis of Penner serotype prevalence of Campylobacter jejuni in low- and middle-income countries." PLOS ONE 16, no. 5 (2021): e0251039. http://dx.doi.org/10.1371/journal.pone.0251039.

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Introduction While Campylobacter jejuni is a leading foodborne bacterial pathogen worldwide, it poses a particular risk to susceptible populations in low- and middle-income countries (LMICs). A capsule-conjugate vaccine approach has been proposed as a potential solution, but little information exists on circulating C. jejuni capsule types in LMICs. The capsule is the major serodeterminant of the Penner typing scheme, which is based on serum recognition of Campylobacter heat-stable antigens. We conducted a systematic review and meta-analysis to estimate the distribution of Penner serotypes asso

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