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Chalupa, Pavel. "Pevnostní kontrola nákladního přívěsu HTV 18." Master's thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2014. http://www.nusl.cz/ntk/nusl-231490.
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The subject of this master's thesis is the strength analysis of the cargo trailer HIPOCAR HTV 18 and proposed modifications. For the strength analysis was selected finite element method. The frame model was modified by the results of the strength analysis. After that has been carried out the strength analysis of the modified frame.
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AZEVEDO, Karinne Silva. "Avaliação da prevalência do Papíloma Humano (HPV) em saliva de pacientes portadores do HIV." Universidade Federal de Pernambuco, 2015. https://repositorio.ufpe.br/handle/123456789/18071.
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Identificar a presença dos sorotipos de alto risco do Papilomavírus Humano (HPV) na saliva de pacientes portadores do vírus HIV. A amostra de 90 pacientes foi oriunda de dois centros de referência em tratamento de ISTs da cidade do Recife, PE, Brasil. Uma entrevista foi realizada para identificar o perfil da amostra, sendo realizada uma coleta de saliva empregando tubos falcon e solução para bochecho com sacarose a 5%, com posterior armazenamento em freezer a -20°C para rastreamento do HPV e genotipagem para o sorotipo 16 e 18 por PCR convencional. Na amostra predominou a presença do sexo masculino 59 de 90 (65,6%), com idade média de 38,8 anos, variando entre 18 e 69 anos, renda familiar média de 1,95 Salários Mínimos (DP = 1,37). A prevalência de HPV nesta amostra foi de 23 de 90 (25,6%) e dos sorotipos 16 e 18 foi 8 de 90 (8,9%). A co-infecção por HPV é comumente observada em pacientes portadores de HIV.
To identify the presence of high-risk serotypes human papillomavirus (HPV) in patients with sexually transmitted infections (STIs). A sample of 90 patients were from two referral hospitals in treatment of STIs. An interview was conducted to identify the sample’s profile a saliva collections being perfomed using falcon tubs and mount rinse with 5% sucrose, subsequente storage in a freezer at -20ºC for HPV screening and genotyping for serotype 16 and 18 by conventional PCR. In the sample predominant male presence 59 of 90 (65.6%) with mean age of 38.8 years, ranging between 18 and 69 years, average family income of 1.95 minimum wages (SD = 1, 37). The prevalence of HPV in this sample was 23 of 90 (25.6%) and the serotype HPV 16 and 18 was 8 of 90 (8.9%). Co-infection with HPV is commonly observed in HIV patients.
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Corden, Sally Anne. "HPV-18 DNA integration in cervical cancer." Thesis, University of Warwick, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.267050.
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Tsuda, Luciana Castelar. "Polimorfismos do fator de necrose tumoral alfa, da interleucina-18 e do interferon gama na coinfecção HIV/HCV." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/22/22132/tde-23112015-192335/.
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As complicações hepáticas secundárias à infecção crônica pelo vírus da hepatite C (HCV) são uma importante causa de morte em portadores da infecção pelo vírus da imunodeficiência humana (HIV). Pacientes com coinfecção HIV/HCV apresentam progressão acelerada da fibrose hepática, na qual há participação da resposta inflamatória do sistema imunológico, e requerem maior atenção no tratamento da hepatite C e de suas reações adversas. Assim, os objetivos principais do estudo foram tipificar e comparar os polimorfismos -607 e -137 da interleucina-18 (IL-18), +874 do interferon gama (IFN-?? e -308 e -238 do fator de necrose tumoral alfa (TNF- ?? em quatro grupos (coinfecção HIV/HCV, monoinfecção pelo HIV, monoinfecção pelo HCV e controles saudáveis); investigar a associação dos alelos e genótipos desses polimorfismos com a resposta ao tratamento da hepatite C (respondedor e não respondedor), graus de atividade necroinflamatória (METAVIR A0A1 vs. A2A3) e de fibrose hepática (METAVIR F0-F2 vs. F3F4) em portadores do HCV e identificar os sinais e sintomas relacionados às reações adversas do tratamento da hepatite C. Os dados foram coletados nos prontuários médicos e no sistema informatizado do Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto e os polimorfismos tipificados pela técnica de reação em cadeia da polimerase com iniciadores de sequência específica. Participaram do estudo 400 indivíduos, distribuídos em quatro grupos de 100, predominantemente constituídos por homens com idade média entre 33 e 50 anos. Na avaliação geral, os genótipos -238 G/G (TNF-?? e +874 A/A (IFN-?? foram mais frequentes no grupo coinfecção HIV/HCV em relação ao monoinfecção pelo HCV. O genótipo -308 G/A e o alelo -308 A (TNF-?? foram associados com a susceptibilidade à coinfecção HIV/HCV e o genótipo -308 G/G e o alelo -308 G (TNF-?), com proteção. No grupo coinfecção HIV/HCV, a frequência do genótipo - 137 G/C (IL-18) foi maior nos sujeitos com atividade necroinflamatória A0A1 que nos com A2A3. Nos pacientes com fibrose F3F4, o genótipo -238 G/G (TNF-?? foi mais frequente no grupo coinfecção HIV/HCV que no monoinfecção pelo HCV e naqueles com F0-F2, o genótipo +874 A/A (IFN-?? também foi mais frequente no grupo coinfecção HIV/HCV. A frequência do genótipo +874 T/T (IFN-??, dentre os pacientes do grupo coinfecção HIV/HCV, foi maior naqueles com fibrose F3F4 que nos com F0-F2. Não foram encontradas associações estatisticamente significantes entre as frequências alélicas e genotípicas e os tipos de resposta ao tratamento da hepatite C nos pacientes do grupo coinfecção HIV/HCV; nos do monoinfecção pelo HCV, houve diferenças nas frequências alélicas e genotípicas (posição -238 do TNF-?) entre pacientes respondedores e não respondedores. Os principais sinais e sintomas relacionados às reações adversas do tratamento da hepatite C foram mialgia, febre, fraqueza, cefaleia e hiporexia. Anemia, hiporexia e vômito foram mais frequentes no grupo coinfecção HIV/HCV. Conclui-se que há relação dos alelos e genótipos de citocinas com a gravidade da doença hepática e resposta ao tratamento da hepatite C. Adicionalmente, algumas reações adversas ao tratamento foram mais pronunciadas em coinfectados HIV/HCVHepatic complications secondary to chronic infection by hepatitis C virus (HCV) are a major cause of death in people infected by the human immunodeficiency virus (HIV). Patients with HIV/HCV coinfection present rapid progression of liver fibrosis, with involvement of the immune system\'s inflammatory response, and require more attention in hepatitis C treatment and its adverse reactions. The main goals of this study were to typify and compare the polymorphisms -607 and -137 of the interleukin-18 (IL-18), +874 of the interferon gamma (IFN-?? and -308 and -238 of the tumor necrosis factor-alpha (TNF-?? in four groups (HIV/HCV coinfection, HIV monoinfection, HCV monoinfection and healthy controls), to investigate the association of the alleles and genotypes of these polymorphisms with response to hepatitis C treatment (responder and non-responder), degrees of necroinflammatory activity (METAVIR A0A1 vs. A2A3) and of liver fibrosis (METAVIR F0-F2 vs. F3F4) in HCV patients and to identify the signs and symptoms related to adverse reactions of hepatitis C treatment. Data were collected on medical records and on the computerized system of the Hospital das Clínicas of the University of São Paulo Ribeirão Preto Medical School and the polymorphisms were typified using the polymerase chain reaction technique with sequence specific primers. The study included 400 individuals, distributed in four groups of 100, predominantly consisting of men with an average age between 33 and 50 years. In the overall evaluation, genotypes -238 G/G (TNF-?? and +874 A/A (IFN-?? were more frequent in the HIV/HCV coinfection group compared to HCV monoinfection. The genotype -308 G/A and allele -308 A (TNF-?? were associated with susceptibility to HIV/HCV coinfection and the genotype -308 G/G and allele -308 G (TNF-?), with protection. In the HIV/HCV coinfection group, the frequency of genotype -137 G/C (IL-18) was greater in subjects with necroinflammatory activity A0A1 than in the ones with A2A3. In patients with fibrosis F3F4, genotype -238 G/G (TNF-?? was more frequent in the HIV/HCV coinfection than in the HCV monoinfection group and in those with fibrosis F0-F2, genotype +874 A/A (IFN-?? was also more frequent in the HIV/HCV coinfection group. The frequency of genotype +874 T/T (IFN-??, among patients of the HIV/HCV coinfection group, was higher in those with fibrosis F3F4 compared to the ones with F0-F2. No statistically significant associations were found between the allele and genotype frequencies and the types of answer to hepatitis C treatment in patients of the HIV/HCV coinfection group. On the ones of the HCV monoinfection group, there were differences on the allele and genotype frequencies (position -238 of TNF-?? among responder and non-responder patients. The main signs and symptoms related to adverse reactions to hepatitis C treatment were myalgia, fever, weakness, headache and loss of appetite. Anemia, loss of appetite and vomiting were more frequent in the HIV/HCV coinfection group. It is concluded that there is relationship of the alleles and genotypes of cytokines with the severity of liver disease and response to hepatitis C treatment. Additionally, some adverse reactions to treatment were more frequent in HIV/HCV coinfected patients
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Colomb, Julie. "Synthèse et radiomarquage de ligands des récepteurs sérotoninergiques 5-HT6 et 5-HT7 pour la tomographie par émission de positons." Thesis, Lyon 1, 2013. http://www.theses.fr/2013LYO10173/document.
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Le développement de radiotraceurs (18F) des récepteurs de la sérotonine 5-HT6 et 5-HT7 pour l'imagerie TEP (tomographie par émission de positons) permettrait d'étudier la fonction et l'implication de ces récepteurs dans des maladies neurodégénératives telles que la schizophrénie ou la maladie d'Alzheimer. A partir des structures et pharmacophores déjà décrits dans la littérature, nous nous sommes orientés vers des dérivés pyrrolidiniques pour les récepteurs 5-HT7 et quinolines pour les récepteurs 5-HT6. 7 radioligands des récepteurs 5-HT7 marqués au fluor 18 ont pu être étudiés par autoradiographie et imagerie μTEP sur le rat et ont montrés des fixations intéressantes, mais avec une sélectivité moyenne du récepteur. 16 ligands du récepteur 5-HT6 ont été synthétisés et 4 d'entre eux ont été radiomarqués afin d'identifier le 2FNQ1P comme radioligand sélectif vis-à-vis du récepteur 5-HT2A (principal récepteur en compétition). Les premières images TEP réalisées sur le chat ont montrées un marquage sélectif dans les zones cérébrales riches en 5-HT6. La poursuite des études biologiques menées en collaboration avec le CERMEP – Imagerie du vivant permettront d'approfondir les caractéristiques de ces nouveaux radioligands synthétisésDevelopment of fluorine 18 labeled radiotracer of 5-HT6 and 5-HT7 receptors for PET imaging (positron emission tomography) allows the study of those receptors in various neurodegenerative diseases such as schizophrenia and Alzheimer disease. Description of structures and pharmacophores in literature led to pyrrolidine derivatives for 5-HT7 receptors and quinolones for 5-HT6. After their synthesis, 7 radioligands of 5-HT7 receptors have been studied by autoradiography and μPET. These radioligands have shown interesting binding on rat, with more or less selectivity for the receptor. 14 ligands of 5-HT6 receptors have been synthesized and 4 have been radiolabeled to select 2FNQ1P as a selective radioligand toward 5-HT2A. First PET images on cat have shown a selective binding in 5- HT6 rich area in brain. Pursue of biological studies, in collaboration with CERMEP – Imagerie du vivant will give more information on those new radioligands
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Schlottmann, Renate. "Zirkulierendes Interleukin-18 im Verlauf der HIV-Infektion und HIV-Hepatitis B- und C-Koinfektion." [S.l.] : [s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=97450727X.
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Wong, Ting-yin, and 王婷妍. "HPV 16 and HPV 18 detection in cytology sample of follicular cervicitis using LAMP assay." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2011. http://hub.hku.hk/bib/B46632761.
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Lautenschläger, Philine. "Konzepte der Leidenschaft : Phädra-Vertonungen im 18. Jahrhundert und das Gattungssystem der tragischen Oper /." Schliengen : Edition Argus, 2008. http://www.editionargus.de/pd1218803042.htm.
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Leung, Sze-ki, and 梁詩琪. "Mechanism of human immunodeficiency virus induced immunedysregulation: TAT & IL-18 interaction." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2005. http://hub.hku.hk/bib/B34605472.
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Correr, Wagner Rafael. "Development of impedimetric DNA sensor for diagnosis of Human Papillomavirus type 18 infection." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/76/76132/tde-05032015-144417/.
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Currently, the most common strategy employed to detect DNA sequences is PCR (Polymerase Chain Reaction). Nevertheless, in the last few years research on DNA biosensors has increased significantly. Such sensors represent an alternative to PCR in the detection of specific DNA sequences, once they exhibit fast response, low limits of detection, and require simpler sample preparation. The development of a biosensor for detection of DNA from Human Papillomavirus type 18 is reported. To immobilise DNA probe onto indium-tin oxide (ITO) electrodes, a silanisation was carried out using 3-Aminopropyltryethoxysilane (APTES). Silanisation was studied and optimised using ultra-violet absorption spectroscopy, atomic force microscopy, fluorescence microscopy, and cyclic voltammetry. After immobilisation, the hybridisation with target sequence is detected by changes in surface properties of ITO electrode by Cyclic Voltammetry and Electrochemical Impedance Spectroscopy, using the Ferri-Ferrocyante redox couple. The detection of synthetic target sequence was performed in the range of 12.5 to 100 nM, and 300nM for PCR products. The sensor did not show significative response for non-complementary sequence at 50 nM. This sensor can be applied for fast and low cost detection of HPV genetic material at nanomolar levels.A estratégia mais empregada atualmente na detecção de sequência de DNA é a PCR (Reação em Cadeira da Polimerase). Contudo, nos últimos anos, a pesquisa em biossensores de DNA tem aumentado significativamente. Estes sensores representam uma alternativa a PCR na detecção de sequências específicas de DNA, uma vez que exibem resposta rápida, baixos limites de detecção e requerem preparação simples da amostra. Nesta dissertação descrito o desenvolvimento de um biossensor para a detecção do DNA do Papilomavirus Humano tipo 18. A fim de imobilizar a sequência de captura de DNA em eletrodos de óxido de estanho e índio (ITO), realizou-se uma silanização usando 3-Aminopropiltrietoxisilano (APTES). A reação de silanização foi estudada e otimizada através das técnicas de Espectroscopia de Absorção Ultravioleta, Microscopia de Força Atômica, Microscopia de Fluorescência e Voltametria Cíclica. Após a imobilização, a hibridização com a sequência alvo é detectada através de alterações nas propriedades de superfície do eletrodo através de Voltametria Cíclica e Espectroscopia de Impedância Eletroquímica, usando o par redox Ferri-ferrocianeto. A detecção da sequência alvo sintética foi realizada no intervalo de 12.5 a 100 nM, e para o produto de PCR, 300 nM. O sensor não demonstrou resposta significativa para sequência não complementar a 50 nM. Este sensor pode ser aplicado na detecção rápida e de baixo custo de material genético do HPV a níveis nanomolares.
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Blalock, Emily Lauren. "Comparative Study of HPV 16 and HPV 18 Antibody Detection in Serum, Cervical Mucus, and Oral Mucosal Transudate." Digital Archive @ GSU, 2008. http://digitalarchive.gsu.edu/biology_theses/19.
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Measuring HPV exposure relies on detection of HPV type-specific antibodies, but methods are not standardized. Additionally, there is little information on the best sample type for HPV antibody detection. This study validated pseudovirion neutralization (PVN) assay for HPV antibody detection and compared it to IgG ELISA. Both assays were applied to paired serum and cervical mucus samples. Additionally, PVN assay was utilized to evaluate the feasibility of oral mucosal transudate (OMT) samples to monitor the HPV immune response. Serum was more likely to be positive on PVN assay than on IgG ELISA (p= 0.025). Both assays correlated with HPV-16 DNA status. HPV-18 PVN assay results correlated with HPV-18 DNA status. Few cervical mucus samples had detectable antibodies; no correlation with HPV DNA status was seen. OMT results were unsatisfactory. PVN assay was more sensitive than IgG ELISA; serum was a more reliable indicator of HPV-16/18 antibody status than cervical mucus.
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Faria, Paola Lara. "Análise de polimorfismos, expressão gênica e níveis séricos de IL-18, IL18BP e IFN-y na infecção crônica pelo HCV e resolução espontânea." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/5/5168/tde-11052015-140253/.
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O curso da infecção pelo HCV é determinado pela competência da resposta imune inata e adaptativa do hospedeiro. A IL-18 é uma citocina pró-inflamatória importante em ambas as respostas imunes e atua sinergicamente com IL-12 induzindo a expressão de IFN-y pelas células T e natural killer. O IFN-? por sua vez possui um papel chave no combate de infecções intracelulares, induzindo um estado antiviral nas células infectadas. O balanço de IL-18 é controlado pela IL18BP, uma citocina importante que atua como um antagonista natural. Estudos mostram que indivíduos cronicamente infectados pelo HCV possuem elevados níveis séricos de IL-18 e IL18BP. Sendo assim, o presente estudo teve como objetivos: 1) determinar o genótipo de polimorfismos de base única (SNPs) localizados nos genes da IL-18 (-607 C > A e -137 G > C), IL18BP (rs2298455 e rs1541304) e IFN-y +874 T > A; 2) quantificar a expressão de seus respectivos mRNAs; e por fim, 3) dosagem dos níveis séricos das respectivas citocinas. Para isto, foram selecionados 51 indivíduos com resolução espontânea e 50 com infecção crônica pelo HCV genótipo 1 que submetidos a técnica de PCR em tempo real para a genotipagem dos polimorfismosIL-18 (-607 C > A e -137 G > C), IL18BP (rs2298455 e rs1541304) e IFN-? +874 T > A; posteriormente foi feita a análise da expressão gênica destes mRNA utilizando como controle endógeno o GAPDH e a dosagem das citocinas foi determinada peta técnica de ELISA. A distribuição dos genótipos nos polimorfismos nos genes IL-18 e IL18BP foram semelhantes nos dois grupos de estudo. No entanto, para o polimorfismo no gene do IFN-?, observamos frequência maior do genótipo TA no grupo de infecção crônica, enquanto que no grupo de resolução espontânea foi mais frequente o genótipo AA (p=0.006). Em contrapartida a expressão gênica nos permitiu observar que nos indivíduos com infecção crônica o mRNA de IL-18 (p < 0.001) e IL18BP (p < 0.001) estavam com uma maior expressão quando comparados com os indivíduos com resolução espontânea, e que isto refletia nas dosagens séricas, onde os indivíduos cronicamente infectados pelo HCV apresentavam altos níveis séricos de IL-18 (p < 0.001) e IL18BP (p=0.012) do que os indivíduos com resolução espontânea. O alelo G foi associado com uma maior produção de IL-18(p=0.02) nos indivíduos com resolução espontânea. Em relação à expressão gênica do mRNA do IFN-y não foi possível observar nenhuma diferença entre os grupos estudados (p=0.322) e a dosagem sérica não foi detectada em ambos os grupos. Os resultados sugerem que apesar do sistema imune ser estimulado durante a infecção pelo HCV, a persistência viral leva a um estado de anergia onde a produção de IFN- y parece ser escassa para uma resposta imune eficaz, sendo que os meios nos quais modulam a expressão gênica do IFN- y ainda parecem obscuros, no entanto já foram descritos mecanismos pós-transcricionais que tem como alvo a região 3\'UTR do mRNA do IFN- y podendo interferir na sua expressãoThe course of HCV infection is determined by the competence of the innate and adaptive immune response of the host. IL-18 is an important proinflammatory cytokine in both immune responses and acts synergistically with IL-12 induces the expression of IFN-y by T and natural killer cells. The IFN-yturn plays a key role in fighting intracellular infections, inducing an antiviral state in infected cells. The balance of IL-18 is controlled by IL18BP, an important cytokine that acts as a natural antagonist. Studies show that individuals chronically infected with HCV have elevated serum levels of IL-18 and IL18BP. Therefore, this study aimed to: 1) determine the genotype of single nucleotide polymorphisms (SNPs) located in genes of IL-18 (-607 C > A and -137 G > C), IL18BP (rs2298455 and rs1541304) and IFN-y +874 T > A; 2) to quantify the expression of their respective mRNAs; and finally 3) the determination of serum levels of the respective cytokines. Fifty-one individuals with spontaneous clearance and 50 were selected with chronic HCV genotype 1 infection who underwent the technique of real-time PCR for genotyping polymorphisms of IL-18 (-607 C > A and -137 G > C), IL18BP (rs2298455 and rs1541304) and IFN-y +874 T > A; later analysis of gene expression of these mRNA was performed using GAPDH as endogenous control and used the ELISA method for the serum of these cytokines. The distribution of genotypes in the IL-18 polymorphisms and IL18BP genes were similar in both study groups. However, for the polymorphism in the IFN-y gene, the genotype most frequently observed in the group of TA chronic infection, whereas in the group of spontaneous clearance AA was more frequent (p = 0.006) genotype. In contrast to gene expression allowed us to observe that in individuals with chronic infection the mRNA of IL-18 (p < 0.001) and IL18BP (p < 0.001) had a higher expression when compared with individuals with spontaneous resolution, and that this reflected in serum using the ELISA technique, where individuals chronically infected with HCV had higher serum levels of IL-18 (p < 0.001) and IL18BP (p = 0.012) than subjects with spontaneous clearance. The G allele was associated with increased production of IL-18 (P = 0.02)in spontaneous clearance group. Regarding the gene expression of IFN-y mRNA was not observed any difference between the groups (p = 0.322) and the serum was not detected in both groups. The results suggest that although the immune system is stimulated during HCV infection leads to viral persistence of anergy a state where the production of IFN-? appears to be scarce for an effective immune response, and the means in which modulate the expression the IFN-y gene still seem unclear, however have been described post-transcriptional mechanisms which target the 3\'UTR of the mRNA of IFN-y may interfere with its expression
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Leung, Sze-ki. "Mechanism of human immunodeficiency virus induced immune dysregulation TAT & IL-18 interaction /." Click to view the E-thesis via HKUTO, 2005. http://sunzi.lib.hku.hk/hkuto/record/B34605472.
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Manoella, Leite dos Santos Cinthya. "Desenvolvimento de um biossensor amperométrico de DNA para detecção do Papilomavírus humano." Universidade Federal de Pernambuco, 2008. https://repositorio.ufpe.br/handle/123456789/1401.
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Previous issue date: 2008Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
O câncer cervical tem sido uma patologia grave no Brasil e no mundo com cerca de 18.680 mulheres infectadas anualmente. O Papilomavírus Humano (HPV) tem sido biologicamente associado ao desenvolvimento de lesões cervicais, devido a sua capacidade de infectar células epiteliais, e progressão ao câncer do colo de útero. O diagnóstico tardio pode inviabilizar o tratamento e levar a óbito muitas mulheres. Este diagnóstico poderia ser antecipado pelo uso de biossensores de DNA, que são dispositivos analíticos que resultam da integração entre uma sonda seqüência específica e um sinal transdutor. Entre outras técnicas eletroquímicas, os biossensores amperométricos têm sido descritos como atrativos devido à sua simplicidade, baixos custos de instrumentação, possibilidade de realização em tempo real e geralmente alta sensibilidade. Neste trabalho, foi desenvolvido um genossensor para detecção de seqüências do vírus HPV usando azul de metileno (AM) como mediador químico. Os sinais biológicos captados foram transduzidos por Voltametria de Pulso Diferencial (VPD). Os eletrodos de trabalho e referência foram produzidos através de técnica de impressão sob uma superfície de polivinil álcool. Os mesmos foram constituídos de carbono e Ag\AgCl, respectivamente. O eletrodo de trabalho foi modificado com quitosana para a imobilização de pequenas seqüências de DNA para hibridização com uma seqüência alvo. A seqüência alvo usada para hibridização foi o gene E6 do HPV18, uma cadeia extensa com 500 pares de bases disponíveis para anelamento com seqüências de 20 bases. Análises de bioinformática foram realizados para verificar possíveis regiões de anelamento destas seqüências curtas ao genoma viral. Os resultados demonstraram um aumento no sinal do AM quando a hibridização ocorreu, evidenciado pelo aumento dos picos de corrente de VPD analisados graficamente. As diferenças entre os sinais emitidos a partir de um eletrodo contendo reações de hibridização e outro isento das mesmas, foi usada para detectar seqüências de DNA viral obtidas de amostras clínicas. Os resultados indicaram ser o método passível de aplicações em diagnósticos clínicos, e a técnica de impressão, um instrumento viável para a construção dos biossensores de DNA utilizando o azul de metileno como indicador de sinais de hibridização
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Nummer, Gerd. "Trotzdem Erwachsen werden: Individuation und Identitätsentwicklung bei jungen Erwachsenen mit Typ-1-Diabetes /." Hamburg : Kovač, 2007. http://www.verlagdrkovac.de/978-3-8300-2800-0.htm.
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Castelar, Luciana. "Polimorfismo da interleucina-18 e do interferon gama na síndrome da lipodistrofia associada à terapia anti-retroviral em portadores do HIV-1." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/22/22132/tde-01042009-112553/.
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A introdução da terapia anti-retroviral de alta potência no tratamento da infecção pelo HIV reduziu significativamente as taxas de morbi-mortalidades relacionadas à imunodeficiência. Entretanto, o tratamento medicamentoso é acompanhado de vários efeitos colaterais, dentre eles, a síndrome da lipodistrofia (SL), caracterizada por alterações morfológicas e metabólicas. Apesar de sua patogenia não estar totalmente esclarecida, é sabido que aumento dos níveis de algumas citocinas inflamatórias estão relacionados com o desenvolvimento da SL. Diversos sítios polimórficos têm sido descritos por influenciarem a transcrição de genes, levando a variações nos níveis de produção de citocinas, como os da região promotora da interleucina-18 (IL-18 -607 C/A e IL-18 -137 C/G) e do gene do interferon gama (IFN- +874 T/A). Diante disso, esse estudo tipificou os polimorfismos da IL-18 e do IFN- em 88 pacientes portadores do HIV com a SL, em 79 portadores do HIV sem a SL, todos sob terapia anti-retroviral e em 133 indivíduos saudáveis, por meio da técnica de reação em cadeia da polimerase com iniciadores de seqüência específica. Este estudo foi aprovado pelo Comitê de Ética em Pesquisa. A presença do alelo -607A e do genótipo -607AA na IL-18 estava significativamente aumentada nos pacientes portadores do HIV com SL quando comparados aos sem a SL, conferindo susceptibilidade ao desenvolvimento da síndrome. De maneira oposta, o alelo -607C e o genótipo -607CC estavam significativamente aumentados em pacientes portadores do HIV sem SL quando comparados aos com a SL, conferindo proteção ao desenvolvimento da síndrome. Os haplótipos -137G/-607A and -137C/-607A, que comportam o alelo -607A, também estavam associados com a susceptibilidade à SL e o haplótipo -137G/-607C estava fortemente associado com proteção contra a SL. Nenhuma diferença significativa na distribuição alélica e genotípica da IL-18 -137 e do IFN- +874 foram observadas entre os grupos de pacientes e o grupo controle. Este é o primeiro estudo que avaliou o polimorfismo da IL-18 e do IFN- na SL e os resultados sugerem que a região promotora da IL-18 está associada com o desenvolvimento da SL em pacientes portadores do HIV.The introduction of highly active antiretroviral therapy in the treatment of HIV infection significantly reduced the rates of morbidity and mortality related to immunodeficiency. However, the drug treatment is accompanied by various side effects, including the lipodystrophy syndrome (LD), characterized by morphological and metabolic changes. Although its pathogenesis is not totally clear, it is known that increased levels of some inflammatory cytokines are related to the development of LD. Several polymorphic sites have been described by influencing transcription of genes, leading the variations in the levels of cytokine production, such as the promoter region of interleukin-18 (IL-18 -607 C/A and IL-18 -137 C/G) and the interferon gamma gene (IFN- +874 T/A). Thus, this study typifies the polymorphism of the IL-18 and IFN- in 88 HIV-infected patients with LD, in 79 HIV-infected patients without LD, all under antiretroviral therapy and in 133 healthy controls, using the sequence-specific primers-polymerase chain reaction. This study was approved by the Ethics Committee at the place of study. The presence of -607A allele and -607AA genotype in IL-18 gene were significantly increased in HIV patients presenting LD as compared with HIV patients without LD, resulting in susceptibility to the development of LD. Conversely, the -607C allele and -607CC genotype were significantly increased in HIV patients without LD as compared with the HIV patients with LD, offering protection against LD. Haplotypes -137G/-607A and -137C/-607A, carrying the -607A allele, were also associated with susceptibility to LD. The haplotype -137G/-607C was strongly associated with protection against LD. No significant differences in IL-18 -137 and IFN- +874 genotype and allele distribution were observed in patients when compared to a control group. This is the first study evaluating the IL-18 and IFN- polymorphisms in LD and the results suggest that the promoter region of the IL-18 gene is associated with LD development in HIV-infected patients.
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Graf-Szczuka, Karola. "Der kleine Unterschied eine Typologie jugendlicher Zeitungsleser und -nichtleser." Hamburg Kovač, 2006. http://www.verlagdrkovac.de/978-3-8300-2959-8.htm.
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Stewart, Tiffanie S. "Lifestyle and Biological Risk Factors for Liver Fibrosis in the Miami Adult Studies on HIV (MASH) Cohort: An HIV Infected and HIV/HCV Co-infected Population." FIU Digital Commons, 2016. http://digitalcommons.fiu.edu/etd/2459.
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Liver disease is now a leading cause of non-AIDS related morbidity and mortality in people living with HIV (PLWH). The present study investigated the interplay between adverse lifestyle factors that are prevalent in PLWH, biological mediators of liver pathogenesis, and a non-invasive measure of liver fibrosis (FIB-4 index) in HIV mono- and HIV/HCV co-infected individuals.
The results of this investigation in the Miami Adult Studies of HIV (MASH) cohort show that the odds of liver fibrosis progression significantly increased over two years for HIV mono-infected participants who drank alcohol hazardously (OR 3.038, P=0.048), and had BMI ≥ 28kg/m2 (OR 2.934, P=0.027). Cocaine use reduced the odds of advancing one stage of liver fibrosis (OR 0.228, P=0.038), but an interaction between high BMI and cocaine use slightly raised the odds by 4.8% of liver fibrosis progression (P=0.072). HIV/HCV co-infected participants showed interactions between cocaine use and high BMI with increased FIB-4 stage (OR 4.985, P= 0.034), however no lifestyle factors could independently predict FIB-4 stage in this group.
Biological mediators previously associated with liver pathogenesis were associated with higher FIB-4 index over 2 years in a subset of (n=65) HIV mono-infected participants. Plasma measures of oxidative stress (% oxidized glutathione: OR 4.342, P= 0.046), hepatocyte-specific apoptosis (Cytokeratin-18 (CK-18): OR 1.008, P=0.021), and microbial endotoxin (lipopolysaccharide (LPS): OR 1.098, P= 0.097) were associated with having higher odds of progressing at least one stage of FIB-4 over 2 years.
The same biological mediators were also associated with liver fibrosis within HIV infected people who also had a harmful lifestyle characteristic. FIB-4 index was significantly associated with % oxidized glutathione in obese subjects (β=0.563, P=0.018), TGF-β1 in cocaine users (β=0.858, P=0.027), and CK-18 in HIV infected individuals without any adverse lifestyle factors (β=0.435, P=0.015).
Taken together, the findings of these studies describe interrelationships between HIV disease status, lifestyle, and biological mediators of liver fibrosis. The results show interactions between lifestyle conditions and the mediators of liver fibrosis may account for higher rates of liver disease in HIV infection. Research is warranted to develop personalized therapeutics for PLWH to curb the burden of liver disease.
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Lung, Mandy Siu Yu Biotechnology & Biomolecular Sciences Faculty of Science UNSW. "The use of Human Papillomavirus promoters to target Cervical Cancer cells." Publisher:University of New South Wales. Biotechnology & Biomolecular Sciences, 2008. http://handle.unsw.edu.au/1959.4/42610.
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Human Papillomavirus (HPV) is one of the most common causes of sexually transmitted disease worldwide. Infections by high-risk HPVs, such as HPV-18, have been associated etiologically with cervical cancer. The successful development of HPV vaccines may be beneficial to the HPV-na??ve population, but women that have already been exposed to the virus are still at risk of developing HPV-associated malignancies. A need for a systemic cure for HPV-infection therefore still exists. Gene therapies using tissue-specific promoters have been reported to be a promising tool for treating cancers; however, few studies have explored this possibility for cervical cancer. The aim of this project is to construct a gene expression vector that can specifically target HPV-infected cervical cancer cells, by making use of the activity and selectivity of the P105 promoter which is determined by transcription control elements within the HPV-18 long control region (LCR). The first part of this study involved the construction of LCR deletion plasmids, and examining the subsequent level of gene expression induced within different mammalian cell lines. The results suggest the LCR to be capable in achieving cervical cancer-specific gene expression. The 3′-end of the viral L1 gene upstream of the LCR appeared to have a repressive effect on the promoter and therefore should be excluded for maximum LCR promoter activity. The second part of the project involved site-directed mutagenesis studies performed on selected transcription factor binding sites with an attempt to further increase the level of LCR promoter activity and specificity towards HPV-infected cervical cancer cells. The results suggest that a GRE/YY1 mutation may significantly enhance promoter activity. In terms of promoter regulation, the E2BSs appeared to be responsible for promoter activation in the absence of viral E2 proteins. The findings of this study suggest a possible gene therapy approach towards the treatment of cervical cancer. By making use of the activity and specificity of the HPV-18 P105 promoter to induce cervical carcinoma-specific expression of appropriate therapeutic genes, suicidal phenotypes can be introduced selectively within HPV-positive cervical cancer cells while normal HPV-negative cells are unaffected.
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Brändle, Fabian. "Demokratie und Charisma fünf Landsgemeindekonflikte im 18. Jahrhundert." Zürich Chronos, 2002. http://deposit.ddb.de/cgi-bin/dokserv?id=2639733&prov=M&dok_var=1&dok_ext=htm.
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梶谷, 直子. "ヒトパピローマウイルス18型E1^E4遺伝子産物の新規機能に関する研究." 京都大学 (Kyoto University), 2014. http://hdl.handle.net/2433/185218.
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Haack, Julia. "Der vergällte Alltag : zur Streitkultur im 18. Jahrhundert." Köln [u.a] Böhlau, 2008. http://deposit.d-nb.de/cgi-bin/dokserv?id=3051839&prov=M&dokv̲ar=1&doke̲xt=htm.
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Pamnani, Shitaldas J. "Incidence, Persistence, and Recurrence of Anogenital α- Mucosal HPV Infections (HPV 6, 11, 16, 18, 31, 33, 45, 52 and 58)." Scholar Commons, 2016. http://scholarcommons.usf.edu/etd/6125.
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Objectives: The aims of this study were to: 1) Assess whether naturally induced anti-HPV antibodies are associated with subsequent acquisition of genital HPV 6, 11, 16, and 18 infections in men, 2) assess the recurrence (redetection) of genital HPV infections of the 9-valent vaccine HPV types and investigate factors associated with recurrent infections among men, and 3) assess the risk of type-specific sequential acquisition of anal HPV infection following a genital HPV infection of the 9-valent vaccine HPV types among men who have sex with women (MSW).
Methods: 4,123 healthy men were followed every six months (median follow-up time 4.1 years). HPV antibodies were measured at baseline using a virus-like particle-based ELISA assay. Genital and anal HPV genotypes were detected using the Roche Linear Array assays. Kaplan-Meier curves and Cox models were developed to assess associations between serum anti-HPV antibody and subsequent incident HPV infections. Individual type analyses and grouped analyses were carried out to assess type-specific recurrence of the 9-valent vaccine HPV types. Risk of sequential anal HPV infection was assessed by examining incident rate ratios (IRR) and adjusted hazard ratios (aHR) among men with a prior genital HPV infection compared to men without a prior genital HPV infection.
Results: 1) Significantly higher rates of incident infections were observed for HPV 16 among baseline HPV 16 seropositive men (aHR 1.37, 95% CI 1.01-1.86). Risk of persistent HPV 18 infection was significantly lower among HPV 18 seropositive men in unadjusted models, but not in the adjusted model, while incident and six-month persistent HPV 6 and 11 infections did not differ by baseline serostatus. 2) Up to 31% of prior prevalent and 20% of prior incident HPV infections recurred over time in individual type analyses. New female sexual partners, frequency of sexual intercourse with female partners, and new male sexual partners were associated with type-specific recurrence of HPV infections (HPV 6, 16, 31 and 58). In grouped analyses, lifetime number of male sexual partners (aOR = 2.40, 95% CI 1.19-4.84) and number of new male sexual partners (aOR 2.35, 95% CI 1.16-4.74) were associated with recurrence of HPV infections. 3) In individual type analyses, men with a prior HPV 16 genital infection had a significantly higher risk of subsequent anal HPV 16 infection (aHR=4.63, 95% CI 1.41-15.23). Significantly higher HRs were observed for any of the nine HPV types (aHR= 2.8, 95% CI1.32-5.99), high risk HPV types (aHR=2.65, 95% CI 1.26, 5.55) and low risk HPV types (aHR=5.89, 95% CI1.29, 27.01) in grouped analyses.
Conclusion: Baseline seropositive status among men was not associated with a reduction in subsequent incident genital HPV 6, 11, and 16 infections, but with a possible protective effect for persistent HPV 18 infections. Men are also susceptible to recurrence of type-specific genital HPV infections, and recurrence of HPV infection was associated with high-risk sexual behaviors. MSW men with prior genital HPV infections are more likely to have a subsequent type-specific anal HPV infection than men who did not have prior genital HPV infections. Understanding the natural history of HPV infections among men is essential to control HPV associated diseases in both men and women.
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Kellner, Katharina. "Pesthauch über Regensburg Seuchenbekämpfung und Hygiene im 18. Jahrhundert." Regensburg Pustet, 2003. http://deposit.ddb.de/cgi-bin/dokserv?id=2706500&prov=M&dok_var=1&dok_ext=htm.
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Mulaga, Rupakisyo Ruthiana. "Identifying and assessing barriers and enablers to HIV counselling and testing among men aged 18-49 in Lilongwe District. A case study of Chileka Health Area." University of the Western Cape, 2021. http://hdl.handle.net/11394/7946.
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Magister Artium (Development Studies) - MA(DVS)Malawi had an estimated HIV prevalence of 9.6% with about 9.2% of its adult population living with HIV/AIDS in 2018. HIV Counselling and Testing (HCT) is essential to the prevention of HIV infection, HIV treatment and access to care and support services. However, despite the availability of free HCT services, the uptake of HCT services has been low among men in Malawi. This study assessed barriers and enablers of HCT uptake among men aged 18-49 in Chileka Health Area in Lilongwe District.
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May, Anja. "Wilhelm Meisters Schwestern Bildungsromane von Frauen im ausgehenden 18. Jahrhundert." Königstein/Taunus Helmer, 2006. http://deposit.ddb.de/cgi-bin/dokserv?id=2740783&prov=M&dok_var=1&dok_ext=htm.
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Detken, Anke. "Im Nebenraum des Textes : Regiebemerkungen in Dramen des 18. Jahrhunderts /." Tübingen : Niemeyer, 2009. http://deposit.d-nb.de/cgi-bin/dokserv?id=3360252&prov=M&dok_var=1&dok_ext=htm.
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Gisler, Monika. "Göttliche Natur? Formationen im Erdbebendiskurs der Schweiz des 18. Jahrhunderts." Zürich Chronos, 2006. http://deposit.d-nb.de/cgi-bin/dokserv?id=2896217&prov=M&dok_var=1&dok_ext=htm.
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Gisler, Monika. "Göttliche Natur? : Formationen im Erdbebendiskurs der Schweiz des 18. Jahrhunderts /." Zürich : Chronos-Verl, 2007. http://deposit.d-nb.de/cgi-bin/dokserv?id=2896217&prov=M&dok_var=1&dok_ext=htm.
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Lehmann-Habeck, Martin. "Intrapsychische Entwicklungen in der Familientherapie bei drogenabhängigen Jugendlichen und jungen Erwachsenen eine kontrollierte Verlaufsuntersuchung an den Indexpatienten, ihren Eltern und Geschwistern." Hamburg Kovač, 2005. http://www.verlagdrkovac.de/3-8300-2001-5.htm.
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Winer, Rachel L. "Genital HPV infection and E7 mRNA viral load : incidence, risk factors, and relations to genital neoplasias /." Thesis, Connect to this title online; UW restricted, 2005. http://hdl.handle.net/1773/10917.
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Vorderstemann, Karin. ""Ausgelitten hast du - ausgerungen ..." lyrische Wertheriaden im 18. und 19. Jahrhundert." Heidelberg Winter, 2006. http://deposit.d-nb.de/cgi-bin/dokserv?id=2926849&prov=M&dok_var=1&dok_ext=htm.
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Braga, Carla Flores. "Identificação de DNA-HPV tipos 16 e 18 e da expressão de p16INK4 em adenocarcinomas de colo uterino." reponame:Repositório Institucional da UCS, 2007. https://repositorio.ucs.br/handle/11338/362.
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O câncer de colo de útero, mundialmente, é um dos mais prevalentes dentre as mulheres. O adenocarcinoma cervical é uma neoplasia cuja origem está nas células da mucosa endocervical. Sua patogênese não está ainda bem compreendida, podendo-se encontrar o vírus HPV como um elemento associado a estas lesões. A prevalência do HPV em adenocarcinomas cervicais apresenta ampla variação. O HPV tipo 16 e, especialmente, o HPV tipo 18, são os tipos mais freqüentemente associados a este tumor. A proteína p16INK4, uma proteína relacionada ao ciclo celular, é superexpressa em muitos tumores humanos, incluindo o carcinoma de células escamosas e o adenocarcinoma cervical. Há evidências que correlacionam esta proteína com infecção por HPV. O objetivo de nosso estudo foi o de identificar o DNA-HPV tipos 16 e 18 e verificar a correlação deste achado com a expressão da proteína p16INK4 em uma série de casos de adenocarcinoma cervical. A identificação do DNA-HPV foi realizada por meio do Nested-PCR e a expressão da proteína p16INK4 foi realizada pela técnica de imunohistoquímica. O DNA-HPV tipo 16 foi o mais freqüente em nossa série (51,4%), seguido pelo DNA-HPV 18 (28,6%). Co-infecção (HPV 16 e HPV 18) foram encontrados em 5,7% dos casos. Uma forte expressão da proteína p16INK4 foi encontrada em associação com positividade para DNA-HPV tipos 16 e 18.Submitted by Marcelo Teixeira (mvteixeira@ucs.br) on 2014-05-22T17:29:12Z No. of bitstreams: 1 Dissertacao Carla Flores Braga.pdf: 962952 bytes, checksum: 3b57ca78ed986451d192ce7b2e10d1b7 (MD5)
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Cervical cancer is one of the most prevalent cancer in women around the world. Cervical adenocarcinoma arises in endocervical mucosal cells and, although HPV is identified in some cases, the pathogenic mechanisms involved in their development are still not understood completely. HPV prevalence in cervical adenocarcinoma varies around the world. HPV 16 and specially HPV 18, are the most frequent types associated with this tumor. The p16INK4, a cell cicle related protein, is superexpressed in many human tumors, including cervical squamous carcinomas. There are findings that correlate this protein with HPV infection. The aim of our study was to identify HPV-DNA and verify the correlation of this findings with the expression of p16INK4 in a series of cervical adenocarcinomas. DNA-HPV analysis was performed by Nested-PCR to HPV 16 and HPV 18 and p16INK4 expression was verified by immunohistochemistry. HPV 16 was the most frequent type in our series (51,4%) followed by HPV 18 (28,6%). Coinfection (HPV16 and HPV 18) was found in 5,7%. A very strong expression of p16INK4 was found in association with HPV-DNA positivity.
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Hadji, Siper. "Vilket vaccin är mest effektivt mot Humant papillomavirus (HPV) -16 och -18, som orsakar livmoderhalscancer - Gardasil eller Cervarix?" Thesis, Linnéuniversitetet, Institutionen för naturvetenskap, NV, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-20368.
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HPV är en grupp av över 100 olika virustyper varav 40 kan infektera könsorganen. Dessa virus orsakar den vanligaste sexuellt överförbara infektionen i Sverige och även i övriga världen. Personer med kvarstående HPV-infektion har en ökad risk att på sikt få cellförändringar och cancer, framför allt livmoderhalscancer. HPV-typ 16 och 18 är de typer som oftast orsakar livmoderhalscancer (omkring 70 % av fallen). Införandet av program för regelbundna gynekologiska cellprovskontroller har under de senaste 40 åren minskat insjuknandet i livmoderhalscancer med drygt 60 procent i Sverige. Dessutom bidrar vaccination mot HPV-infektionerna, vilket ingår i det allmänna barnvaccinationsprogrammet sedan den 1 januari 2010, till att förebygga livmoderhalscancer. Det finns idag två vacciner som skyddar mot HPV- infektion. Båda dessa vacciner är riktade mot HPV 16 och 18. Det ena vaccinet, Gardasil, ger även skydd mot kondylom som orsakas av HPV- typ 6 och 11 och det andra, Cervarix, ger ett visst skydd mot några andra HPV-typer som kan orsaka cancer i livmoderhalsen. Syftet med detta arbete har varit att undersöka vilket av vaccinerna Gardasil eller Cervarix som är mest effektivt, vilket mäts som halten vaccinspecifika antikroppar. Immunogeniciteten av en eller två doser av vaccinen gentemot tre doser har också undersökts och dessutom har effekten av alternativa doseringsschema i förhållande till standarddoseringsschema undersökts för både Cervarix och Gardasil. Arbetet är en litteraturstudie omfattande sex studier över effekter av Gardasil och Cervarix. I två av artiklarna jämfördes vaccinen med varandra, medan resterade fyra behandlades skillnaden mellan tre kontra två doser och hur alternativa scheman för HPV-vaccinen fungerar jämfört med avsedda vaccinationsscheman. De båda profylaktiska vaccinerna, Cervarix (HPV-16/18 vaccinet) och Gardasil (HPV-6/11/16/18 vaccinet), mot HPV är effektiva och tolereras väl. Cervarix-vaccinet har dock generellt högre immunogenicitet än Gardasil och var signifikant bättre i alla åldersgrupper (p <0,0001). Vid studier av olika doseringsscheman, visar det sig att den tredje vaccindosen kan administreras när som helst mellan månad 6 och 12 efter den första dosen för båda vaccinen. Den andra dosen av Gardasil kan administreras enligt standardschemat, dvs vid månad 2 eller enligt alternativt schema vid månad 3 eller månad 6. Det går utmärkt att bortse från den tredje dosen av Cervarix eftersom två-dos alternativet är mycket effektivt för att förebygga infektioner orsakade av HPV 16 och 18. Data tyder på att samma gäller för Gardasil, men inga studier har utförts specifikt för att jämföra tre kontra två doser av detta vaccin. Flexiblare doseringsschema med 2-dos-regim kan minimera kostnaderna, öka möjligheterna för fler att vaccineras och minska frekvensen av livmoderhalscancer.
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Roncato, Gerusa Cristhiny da Paixão. "Detecção de Papilomavírus Humano (HPV) em Adultos Jovens com idades entre 18 a 25 anos do Município de Leopoldo de Bulhões-GO." Pontifícia Universidade Católica de Goiás, 2011. http://localhost:8080/tede/handle/tede/2333.
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Previous issue date: 2011-04-14Genital infection by human papillomavirus (HPV) is considered the sexually transmitted disease most prevalent among the population. The man has represented an important role as virus reservoir and transmitter. Most HPV-infected men are asymptomatic. The HPV infection prevalence in young males is poorly understood and few studies are available. This study aim was to evaluate the HPV prevalence in a group of young adults, ages 18 and 25, male, living in the City of Leopoldo de Bulhões-GO. For viral genome detection, was used the polymerase chain reaction (PCR) technique with primers PGMY09/11 Line Blot. Descriptive statistics and their frequencies was performed for the variables related to socio-demographic and behavioral features in the group of asymptomatic men for HPV infection. To investigate the possible associations between related factors and the infection, univariate analysis was performed by chi-square test with Yates correction. The study involved a population of 57 asymptomatic men aged between 18 and 25 years old living in the city of Leopoldo de Bulhões-GO, selected at the Jose Francisco Vargas Health Center. Among the study group behavioral characteristics, 43.9% of subjects were single and 56.1% were married. The individual s age who initiated sexual activity was approximately 15 years old. All subjects were informed about the study and signed a free and clear consent form. The HPV infection prevalence in the study group was 31.6%. HPV infection was significantly associated with marital status and partner s number, in other words, infection was more prevalent among unmarried individuals (56%) who did not have a steady partner (57.1%). As for condom use and early sexual activity, HPV infection was not significantly associated. Our results demonstrate that a man behavioral factor represents a significant risk for HPV infection association
A infecção genital pelo papilomavírus humano (HPV) é considerada a doença sexualmente transmissível de maior prevalência na população mundial. O homem tem apresentado um importante papel como reservatório e o transmissor desse vírus. A maioria dos homens infectados pelo HPV são assintomáticos. A prevalência da infecção pelo HPV em jovens do sexo masculino é pouco conhecida e apenas alguns estudos encontram-se disponíveis. O objetivo deste trabalho foi avaliar a prevalência do HPV em um grupo de adultos jovens, com idades entre 18 e 25 anos, do sexo masculino, residentes no Município de Leopoldo de Bulhões-GO. Para a detecção do genoma viral utilizou a reação em cadeia da polimerase (PCR), utilizando a técnica com primers PGMY09/11. A estatística descritiva com suas respectivas freqüências foi realizada para as variáveis relativas às características sócio-demográficas, comportamentais no grupo de homens assintomáticos para infecção pelo HPV. Para investigar as possíveis associações entre os fatores relacionados e a infecção realizou-se análise univariada, pelo teste do qui-quadrado com correção de Yates, selecionados no Centro de Saúde José Franscisco Vargas. Dentre as características comportamentais do grupo estudado 43,9% dos indivíduos eram solteiros e 56,1% eram casados. A idade de início da atividade sexual foi de aproximadamente 15 anos. Todos os indivíduos foram informados sobre o estudo e assinaram o termo de consentimento livre e esclarecido. A prevalência da infecção pelo HPV no grupo estudado foi de 31,6%. A infecção pelo HPV esteve significativamente associada ao estado civil e ao número de parceiras, ou seja, a infecção foi mais prevalente nos indivíduos solteiros (56%) e que não apresentaram parceira fixa (57,1%). Quanto ao uso do preservativo e o início da atividade sexual a infecção pelo HPV não esteve significativamente associada. Nossos resultados demonstraram que fatores comportamentais do homem representam uma associação com risco significativo para a infecção pelo HPV
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Nummer, Gerd. "Trotzdem erwachsen werden: Individuation und Identitätsentwicklung bei jungen Erwachsenen mit Typ-1-Diabetes." Hamburg Kovač, 2006. http://www.verlagdrkovac.de/978-3-8300-2800-0.htm.
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Barth, Thomas. "Adelige Lebenswege im Alten Reich der Landadel der Oberpfalz im 18. Jahrhundert." Regensburg Pustet, 2002. http://deposit.ddb.de/cgi-bin/dokserv?id=2641175&prov=M&dok_var=1&dok_ext=htm.
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Rothe, Matthias. "Lesen und Zuschauen im 18. Jahrhundert die Erzeugung und Aufhebung von Abwesenheit." Würzburg Königshausen und Neumann, 2005. http://deposit.ddb.de/cgi-bin/dokserv?id=2683383&prov=M&dok_var=1&dok_ext=htm.
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Sampaio, Sarah Carvalho de Alencar. "Identificação de HPV de alto risco oncogênico em citologia em meio líquido com atipias escamosas e carcinoma escamoso." reponame:Repositório Institucional da UFC, 2015. http://www.repositorio.ufc.br/handle/riufc/14606.
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SAMPAIO, Sarah Carvalho de Alencar. Identificação de HPV de alto risco oncogênico em citologia em meio líquido com atipias escamosas e carcinoma escamoso. 2015. 57 f. Dissertação (Mestrado em Patologia) – Faculdade de Medicina, Universidade Federal do Ceará, Fortaleza, 2015.Submitted by denise santos (denise.santos@ufc.br) on 2015-12-21T12:12:32Z No. of bitstreams: 1 2015_dis_scasampaio.pdf: 475142 bytes, checksum: 0889054dafdd6b2e0a27be354b1ffb64 (MD5)
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To identify high-risk HPV genotypes in liquid-based cytology smears(SurePath®) with diagnosis of squamous atypia and squamous carcinoma. METHODS: This was a cross-sectional study using 165 liquid-based cytology with squamous atypia and 149 without atypia. HPV genotyping by real-time PCR was performed on this material. The material was processed by COBAS® 4800 System (Roche), which has three detection channels: HPV 16, HPV 18, and HPV HR (other twelve high-risk genotypes). RESULTS:We analyzed 75 cases of ASC-US, 62 LSIL, 8 ASC-H, 12 HSIL and 8 squamous carcinomas. The average age was 32.2 years in the group without atypia, 31.1 years for ASC-US and LSIL, 41.2 years for ASC-H and HSIL and 43.1 years for SCC. There were 112 positive cases for HPV (68%) in the group with atypia: 72% positive for HPV AR, 18% of HPV16 and 10% of HPV 18. Only in ASC-US group, HPV positive and negative frequency was similar (1:1). In the others, the amount of HPV positive cases surpassed negative ones. In the group of Cytology without atypia were found 40 positive cases for HPV (26%): 68% positive for HPV AR probe, 17% of HPV18 and 15% of HPV16. Detections by a single probe predominated in both groups (88% and 84%). In the group with atypia the most prevalent combination was HPV16 and HPV HR (57%); and in cases without atypia was HPV18 and HPV HR (68%) and there was a single positive case for three channels in this group. CONCLUSIONS: The prevalence of other 12 high-risk HPV genotypes (not 16 and 18), was frequent in cytology with and without squamous atypia, associated or not with genotypes 16 and 18. The connection of squamous atypia with HPV 16 and HPV HR was significant. The data obtained are in agreement with the literature regarding the existence of heterogeneity in the distribution of different genotypes and their most frequent association as the level of atypia.
Identificar genótipos de HPV de alto risco em citologias em meio líquido com diagnóstico de atipias escamosas e carcinoma escamoso. MÉTODOS: Foi realizado estudo de corte transversal utilizando 165 citologias em meio líquido com atipias escamosas e 149 sem atipias. Nesse material foi realizado genotipagem do HPV por PCR em tempo real. O material foi processado pelo Sistema Cobas® 4800 (Roche), que apresenta três canais de detecção, para HPV 16, HPV 18, e HPV AR (outros doze genótipos de HPV de alto risco). RESULTADOS: Foram analisados 75 casos de ASC-US, 62 LSIL, 8 ASC-H, 12 HSIL e 8 carcinomas escamosos. A média etária foi de 32,2 anos no grupo sem atipias, 31,1 anos para ASC-US e LSIL, 41,2 anos para ASC-H e HSIL e 43,1 anos para CEC. Houve 112 casos positivos para HPV (68%) no grupo com atipias, sendo 72% de positividade para HPV AR, 18% de HPV 16 e 10% de HPV 18. Somente nos casos de ASC-US a frequência de HPV positivo e negativo foi semelhante (1:1). Nas demais, a quantidade de casos HPV positivo ultrapassou a de negativos. No grupo de citologias sem atipias, foram encontrados 40 casos positivos para HPV (26%), sendo 68% de positividade para a sonda HPV AR, 17% de HPV 18 e 15% de HPV16. Predominaram detecções por uma única sonda em ambos os grupos (88% e 84%). No grupo com atipias, a combinação mais presente foi HPV AR e 16 (57%); e nos casos sem atipias, foi HPV AR e 18 (68%) e houve um único caso positivo para os três canais neste grupo. CONCLUSÕES: A prevalência de outros 12 genótipos de alto risco de HPV (que não 16 e 18), foi frequente, em citologias com e sem atipias escamosas, associados ou não aos genótipos 16 e 18. A relação de atipias escamosas com HPV 16 e HPV AR foi significativa. Os dados obtidos estão em concordância com a literatura quanto à existência de uma heterogeneidade na distribuição dos diversos genótipos e sua associação mais frequente conforme o nível de atipia.
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Oehler, Nicola [Verfasser], and Maura [Akademischer Betreuer] Dandri-Petersen. "Das "Metabolovirus" HBV : Auswirkungen einer chronischen Hepatitis B-Infektion auf den hepatozellulären Lipid-, Cholesterin- und Gallensäuremetabolismus / Nicola Oehler. Betreuer: Maura Dandri." Hamburg : Staats- und Universitätsbibliothek Hamburg, 2016. http://nbn-resolving.de/urn:nbn:de:gbv:18-77699.
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Wendt, Eva-Verena. "Sexualität und Bindung : Qualität und Motivation sexueller Paarbeziehungen im Jugend- und jungen Erwachsenenalter /." Weinheim ; München : Juventa, 2009. http://deposit.d-nb.de/cgi-bin/dokserv?id=3124824&prov=M&dok%5Fvar=1&dok%5Fext=htm.
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Meissner, Joachim. "Mythos Südsee das Bild von der Südsee im Europa des 18. Jahrhunderts." Hildesheim Zürich New York Olms, 1998. http://deposit.d-nb.de/cgi-bin/dokserv?id=2883872&prov=M&dok_var=1&dok_ext=htm.
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Carvalho, Larissa Fernandes de. "ASSOCIAÇÃO DE HPV EM CARCINOMAS ESPINOCELULARES DE PÊNIS: UMA META-ANÁLISE." Pontifícia Universidade Católica de Goiás, 2010. http://localhost:8080/tede/handle/tede/2427.
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Previous issue date: 2010-05-31Cancer has been a major public health problem both in developed and developing countries . Each year, cancer is responsible for more than six million deaths, imposing a population burden that claims about 12% of all causes of that worldwide. Under a genetic perspective, there are two major classes of gene that hold an important role on cancer development: the proto-oncogenes and the tumor suppress genes. Mutations in these genes are the underlying cause of uncontrolled cell proliferation. Under the spectrum of male urological tumors, penile carcinoma is the one that most resembles cervical carcinoma in women. Epidemiological studies have frequently implicated HPV genome as a potential initiator factor for carcinomas of different anatomical sites. HPV role in cancer induction is well documented for cervical carcinomas and a significant association has been found in up to 99,7% of all cases worldwide. The occurrence of HPV in both male and female genital areas has increased the scientific interest around the potential association of HPV genome and penile carcinomas. The HPV genome is mode of one copy of the double-stranded circular DNA molecule, organized in three distinct regions: a Long Control Region (LCR), an Early (E) and a Late (L) protein coding regions. Following viral insertion in a cell genome, the viral LCR becomes the target of an intracellular regulatory mechanism. When infected cells lose this control mechanism, tumor progression towards malignancy and increased viral gene expression occur. Penile cancer is a rare disease, however by far the most common type is the squamous cell carcinoma which is found in about 95% of the penile malignancies. As any other Sexually Transmitted Disease, men are the major link in the chain of infection of HPV. The Polimerase Chain Reactions is the most sensitive procedure to detect and genotype HPV DNA in human biological samples. The current study is a meta-analysis which comprised the result of several studies that using PCR detected and genotyped HPV genome in association with penile cancers. Herein we report on the results of 39 research articles published from 1989 to 2009. We found the published data had large heterogeneity, tending to significance when combined within the 95% confidence interval. The DermonSaimonian-Laird analysis indicated significance between HPV infection and penile cancer development.
O HPV atua como uma importante causa de câncer cervical, em cerca de 99,7% dos casos em todo mundo. Como ocorre em toda infecção de transmissão sexual, o homem é o principal elo na cadeia epidemiológica do HPV e tem sido bastante discutido a associação deste vírus aos carcinomas penianos. O câncer de pênis é uma doença rara, sendo o carcinoma de células escamosas responsável por 95% dos casos. Assim, a infecção por HPV em homens, promove modificações bioquímicas e moleculares, alterando significativamente a população de células, através da interação do genoma viral com o genoma da célula hospedeira ou de proteínas virais com proteínas celulares necessárias ao controle do ciclo celular, como as proteínas supressoras de tumor pRb e p53, podendo desencadear a progressão para o processo maligno. Dentre as técnicas moleculares para detecção de DNA do HPV, a PCR é a mais sensível, pois é capaz de identificar o tipo do HPV. Neste contexto, o presente estudo teve como objetivo, investigar a associação entre o HPV e os pacientes com câncer de pênis através de uma meta-análise. Um procedimento destinado a examinar, de modo simultâneo, os resultados de várias investigações sobre um mesmo tópico, de forma a gerar conclusões com maior segurança e confiabilidade. De um total de quarenta e quatro (44) artigos encontrados, foram incluídos na meta-análise, trinta e nove (39) artigos e duas (2) dissertações entre os anos de 1989 a 2009 que estabeleciam a co-relação e utilizavam as mesmas técnicas e primers para detecção e genotipagem do HPV. Na presente meta-análise, observou-se que os artigos analisados apresentaram heterogeneidade quando avaliados os tipos de HPV 16 e 18, em conjunto. O HPV do tipo 18 isolado não apresentou significância, enquanto o HPV 16 demonstrou estar significativamente relacionado ao carcinoma espinocelular de pênis. Pelo teste de DerSimonian-Laird os resultados dos estudos combinados demonstram que os mesmos são significativos e heterogêneos em relação a IC 95%. Assim, pode-se concluir que a associação entre câncer de pênis e HPV se confirma por meta-análise, demonstrando assim a importância da força estatística da meta-análise diante dos estudos isolados na tentativa de desenvolvimento de novas estratégias de prevenção do HPV.
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Ley, Lisa Maria. "Kunst im Zeichen der Aufklärung : Sergels Menschenbild vor dem Hintergrund philosophischer, historischer, gesellschaftspolitischer und psychologischer Ideen des 18. Jahrhunderts." Hamburg Kovač, 2007. http://www.verlagdrkovac.de/978-3-8300-2751-5.htm.
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Ley, Lisa Maria. "Kunst im Zeichen der Aufklärung Sergels Menschenbild vor dem Hintergrund philosophischer, historischer, gesellschaftspolitischer und psychologischer Ideen des 18. Jahrhunderts." Hamburg Kovač, 2006. http://www.verlagdrkovac.de/978-3-8300-2751-5.htm.
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Mosner, Marc Philipp Christian. "Die typenspezifische humorale Immunantwort gegen verschiedene HPV-Kapside (HPV-L1 6, 11, 16, 18, 31, 33, 35, 45) im Zusammenhang mit dem Nachweis von typenspezifischer HPV-DNA bei Frauen mit rezidivierenden Zervixdysplasien." Diss., lmu, 2004. http://nbn-resolving.de/urn:nbn:de:bvb:19-27536.
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Bracht, Johannes. ""Reidung treiben" wirtschaftliches Handeln und sozialer Ort der märkischen Metallverleger im 18. Jahrhundert." Münster Ardey-Verl, 2000. http://deposit.ddb.de/cgi-bin/dokserv?id=2640934&prov=M&dok_var=1&dok_ext=htm.
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Hahn, Stephanie. "Fulda "Zur Zierde der Stadt" ; Bauten und Bauaufgaben der Residenzstadt im 18. Jahrhundert." Petersberg Imhof, 2004. http://deposit.ddb.de/cgi-bin/dokserv?id=2642827&prov=M&dok_var=1&dok_ext=htm.
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Hahn, Stephanie. "Fulda "Zur Zierde der Stadt" : Bauten und Bauaufgaben der Residenzstadt im 18. Jahrhundert /." Petersberg : Imhof, 2006. http://deposit.ddb.de/cgi-bin/dokserv?id=2642827&prov=M&dok_var=1&dok_ext=htm.
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Moderow, Hans-Martin. "Volksschule zwischen Staat und Kirche das Beispiel Sachsen im 18. und 19. Jahrhundert." Köln Weimar Wien Böhlau, 2006. http://deposit.d-nb.de/cgi-bin/dokserv?id=2893885&prov=M&dok_var=1&dok_ext=htm.
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