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1

Holopírková, Lucie. "Iontové mikroklima budov v architektonickém návrhu." Doctoral thesis, Vysoké učení technické v Brně. Fakulta architektury, 2019. http://www.nusl.cz/ntk/nusl-403853.

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One of the main priorities of our society is to constantly increase the quality of life. Part of the new trends and technology is to optimize all factors affecting each individual in buildings at home, and in the work environment. Modern technology increasingly find inspiration in traditional materials and principles of architectural design. We can greatly affect the microclimate of buildings by suitable selection of material surfaces, ventilation systems and selection of structures that can affect the natural electromagnetic field of the Earth during the architectural design of buildings. And
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2

Awadh, Ammar A. "Biofilm formation and characterisation in human mycoplasmas." Thesis, Kingston University, 2014. http://eprints.kingston.ac.uk/32196/.

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Mycoplasmas are the smallest and simplest self-replicating prokaryotes, lacking a cell wall and are bound by a single membrane-the plasma membrane. Moreover, mycoplasmas are consequential and chronic pathogens of humans and animals that rely on adhesion to host tissue for colonisation and infections. In other word biofilm formation is correlated with infections and because of this, the biofilm formation of Mycoplasma species has been investigated and characterised in this project. The viability of biofilm formation was assessed and quantified using crystal violet assay over 20-day incubation p
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Berhane, Nahom Ahferom. "Antimicrobial Proteins for Human Health." Thesis, Université d'Ottawa / University of Ottawa, 2018. http://hdl.handle.net/10393/37283.

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Bacteria are one of the largest causes of human disease, with millions of deaths every year attributed to bacterial infections, and they have become more difficult to tackle with the widespread emergence of antibiotic resistance. In this thesis, I describe my studies that pursued two approaches: one focus was on using antimicrobial histones as an alternative to treatment for antibiotic resistant bacteria; in another approach the recombinant version of an eggshell cuticle protein was expressed and purified for testing against food-safety pathogens. One major pathogen that is contributing to t
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4

Azarnoush, Kian. "The Effects of Amixicile on Sub-gingival Biofilm Cultured from Humans." VCU Scholars Compass, 2018. https://scholarscompass.vcu.edu/etd/5349.

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Periodontitis is an inflammatory disease of the oral cavity induced by anaerobic bacteria, that remains to be the primary cause of tooth loss in adults worldwide. Finding an anti-microbial therapeutic to selectively target periodontal pathogens has proven to be difficult, and current treatment modalities only provide a transient benefit. Amixicile is a non-toxic, readily bioavailable novel antimicrobial that targets strict anaerobes through inhibition of the activity of Pyruvate Ferredoxin Oxidoreductase (PFOR), a major enzyme mediating oxidative decarboxylation of pyruvate, a critical step in
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Secor, Patrick Robert. "Impact of Staphylococcus aureus biofilm conditioned medium on inflammation and epithelialization in human keratinocytes." Diss., Montana State University, 2011. http://etd.lib.montana.edu/etd/2011/secor/SecorP0511.pdf.

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Chronic wounds are characterized by prolonged inflammation and failure to epithelialize and do not respond well to conventional treatment. Bacterial biofilms are a major impediment to wound healing. The effective treatment of chronic wounds requires a better understanding of the relationship between bacterial biofilms and human skin. Human keratinocytes are the most abundant cell type in the epidermis and play essential roles in the inflammatory and epithelialization phases of wound repair. The aim of this dissertation was to determine the effect of biofilm-conditioned medium (BCM) and plankto
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Yi, Song. "A unique relationship between switching, mating and biofilm formation in the human pathogen Candida albicans." Diss., University of Iowa, 2009. https://ir.uiowa.edu/etd/324.

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Candida albicans is the most prevalent human fungal pathogen. The research described in this thesis has focused on the identification and characterization of the regulatory pathways in this pathogen controlling white-opaque switching, mating and biofilm formation as well as the relationship between them. White-opaque switching and mating in C. albicans are under the repression of the a1-α2 complex. Based on this, a chromatin immunoprecipitation-microarray analysis of the a1-α2 target genes was conducted to search for the master switch locus. The result identified TOS9 (WOR1) as a master regula
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7

Yi, Song Soll David R. "A unique relationship between switching, mating and biofilm formation in the human pathogen Candida albicans." Iowa City : University of Iowa, 2009. http://ir.uiowa.edu/etd/324.

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8

Probert, Hollie Marie. "An investigation of the lumenal and mucosal microflora of the human colon : effects of prebiotics on bacteriology and gas generation." Thesis, University of Reading, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.252247.

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9

Inizan, Catherine. "Human Immunodeficiency Virus Type I subverts T cell extracellular matrix to shelter cell-associated infectivity in a viral biofilm." Thesis, Paris 6, 2015. http://www.theses.fr/2015PA066437/document.

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La dissémination du VIH-1 par contacts cellulaires est plus efficace que sa transmission par particules virales libres. Cependant, la nature du matériel infectieux transféré à la jonction reste très mal connue. Nos travaux révèlent que l'infectivité du VIH-1 associée aux lymphocytes T est majoritairement portée à la surface cellulaire dans un biofilm viral. Initialement décrit pour le rétrovirus lymphotrope HTLV-1 (Human T-cell Leukemia Virus type-1), le biofilm viral est une colonie extracellulaire de particules virales infectieuses enchâssées dans un cocon de matrice extracellulaire (MEC). P
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10

Bowler, Laura. "Pseudomonas aeruginosa biofilm and planktonic bacteria display different virulence mechanisms when co-cultured with human A549 lung cells using the Calgary Biofilm Device co-culture system." American Society for Microbiology, 2012. http://hdl.handle.net/1993/31262.

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Cystic Fibrosis (CF) is the most common hereditary genetic disorder among Caucasians. Pseudomonas aeruginosa is a major cause of morbidity in cystic fibrosis patients. Chronic infection with P. aeruginosa eventually occurs and is associated with a switch to biofilm formation of the bacteria. The symptoms and pathology of acute and chronic P. aeruginosa infections differ greatly. The first line of defense within the lung is the physical barrier of the lung epithelia. The examination of established biofilm interactions with lung epithelia is difficult. Here, I use the Calgary Biofilm Device co-c
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11

Bastos, Marília Camotti. "Study of the environmental contamination of human and veterinary medicines in the south Brazil." Thesis, Poitiers, 2017. http://www.theses.fr/2017POIT2255/document.

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Le sud du Brésil est une région agricole qui connait une forte croissance de sa production tant au niveau céréalier qu'animal. L'intensification des pratiques agricoles ainsi que l'urbanisation grandissante dans cette région génèrent également une forte pression anthropique pour les milieux aquatiques et les sols. Le présent travail vise à mieux comprendre l'impact lié à l'utilisation puis au rejet de composés pharmaceutiques (humain ou vétérinaire) sur les rivières et les sols à travers l'exemple du bassin versant du Rio Guaporé. L'étude des sols a montré une contamination par les composés ph
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12

Wood, Cecily R. "Light sensing in a human pathogen: genetic, biochemical, functional and proteomics analyses of blue light regulation in Acinetobacter baumannii." Miami University / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=miami1556139448394676.

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13

Wermser, Charlotte [Verfasser], and Daniel [Gutachter] Lopez. "Morphology, regulation and interstrain interactions in a new macrocolony biofilm model of the human pathogen Staphylococcus aureus / Charlotte Wermser ; Gutachter: Daniel Lopez." Würzburg : Universität Würzburg, 2019. http://d-nb.info/1192216385/34.

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14

Pais, Correia Ana Monica. "Biofilm-like extracellular viral assemblies mediate HTLV-1 (human T cell leukemia virus type-1) cell-to-cell transmission at virological synapses." Paris 7, 2009. http://www.theses.fr/2009PA077233.

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Près de 20 millions d'individus sont infectés par HTLV-1 (human T cell leukemia virus type-1). La plupart des patients n'ont aucun symptôme, seulement 5-10% d'entre eux développeront des maladies associées à l'infection virale. La particularité d'HTLV-1 est de se transmettre par des contacts cellulaires désignés « synapses virologiques », qui partagent certaines caractéristiques avec les synapses immunologiques. Ce projet a permis de mieux comprendre le processus de transmission cellule à cellule et introduit un nouveau concept de transmission virale par des « biofilms viraux ». Contrairement
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15

Crowther, Grace. "Development and characterisation of an in vitro human gut model to study the biofilm mode of growth of Clostridium difficile and the indigenous gut microbiota." Thesis, University of Leeds, 2013. http://etheses.whiterose.ac.uk/5736/.

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Clostridium difficile infection (CDI) is associated with significant patient morbidity, mortality and financial burden. Until recently, antimicrobial treatment options were limited to metronidazole and vancomycin, but both agents are associated with recurrence rates of approximately 20%. The human gastrointestinal tract harbours a complex microbial community which exist in planktonic and sessile form. Sessile organisms are known to cause chronic infection such as cystic fibrosis. Mucosal biofilms exist on surfaces of the gastrointestinal tract, but the existence and role of C. difficile in the
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16

Weckel, Antonin. "Molecular mechanisms of Streptococcus pyogenes tissue colonization and invasion The N-terminal domain of the R28 protein promotes emm28 Group A Streptococcus adhesion to host cells via direct binding to three integrins Group A Streptococcus efficiently colonizes and invades a human tissue and limits its immune response during the early steps of infection." Thesis, Sorbonne Paris Cité, 2018. https://wo.app.u-paris.fr/cgi-bin/WebObjects/TheseWeb.woa/wa/show?t=2090&f=14189.

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Streptococcus pyogenes, également appelé Streptocoque du Goupe A (SGA), est un pathogène à l'origine d'une grande diversité d'infections, allant d'infections superficielles comme l'angine aux infections invasives, comme la fasciite nécrosante et les endométrites. Au 19ème siècle, une femme sur dix mourait après l'accouchement de fièvre puerpérale, notamment d'endométrite. En France, les infections gynéco-obstétricales correspondent encore de nos jours à 27 % des infections invasives à SGA chez les femmes. Les souches de SGA présentent une forte diversité génétique et de répertoire de facteurs
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17

Fromantin, Isabelle. "Etude de la flore bactérienne dans les plaies tumorales du sein : incidence des biofilms bactériens sur l'évolution des plaies et le développement d'odeurs." Phd thesis, Université de Cergy Pontoise, 2012. http://tel.archives-ouvertes.fr/tel-00838471.

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La plaie tumorale est une infiltration par des cellules tumorales du tissu cutané, des vaisseaux sanguins et/ou lymphatiques. Ces plaies peuvent se présenter sous des aspects très différents accompagnés de symptômes variés. Outre les risques d'infection locale, les odeurs nauséabondes posent des problèmes récurrents d'isolement social, de difficultés conjugales et entrainent une sensation de dégout, de pourriture, voire de mort imminente. Elles influencent même le comportement médical. La colonisation bactérienne, les odeurs et les risques infectieux semblent intimement liés chez ces patients
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18

Lembré, Pierre. "Contribution à l'étude de l'organisation et des propriétés mécaniques d'exopolymères de matrice de biofilms modèles." Phd thesis, Université de Cergy Pontoise, 2012. http://tel.archives-ouvertes.fr/tel-00844745.

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Les biofilms sont des édifices macromoléculaires qui résultent de l'adhérence de microorganismes à une surface. Ils sont constitués de cellules enchâssées dans un réseau d'exopolymères d'origine biologique qui forment une matrice extracellulaire. Les biofilms posent des problèmes technologiques et sanitaires dans de nombreux domaines, aussi bien agroalimentaire, médical, ou industriel. Comprendre les mécanismes de formation de ces structures est donc un enjeu majeur. Malgré une grande diversité de la structure des biofilms, de grands principes semblent en régir la composition. Ainsi, la présen
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19

Saussereau, Emilie. "Utilisation des bactériophages comme thérapie lors d'une infection à Pseudomonas aeruginosa dans le cadre de la mucoviscidose : efficacité et innocuité." Phd thesis, Université Pierre et Marie Curie - Paris VI, 2012. http://tel.archives-ouvertes.fr/tel-00836178.

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Face au phénomène de multi-résistance aux antibiotiques des souches de Pseudomonas aeruginosa chez les patients atteints de mucoviscidose, de nouvelles approches doivent être envisagées. L'utilisation des bactériophages pour cibler les bactéries semble être l'une des plus prometteuses. L'efficacité de la phagothérapie semble démontrée par son utilisation en Europe de l'Est depuis des décennies et par les récents résultats obtenus sur des modèles expérimentaux. Cependant, la possibilité de son utilisation chez des patients atteints de mucoviscidose n'a pas encore fait l'objet d'études approfond
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20

Soares, Pedro Miguel Oliveira. "Determination of the importance of iron acquisition for Staphylococcus epidermidis biofilm survival in human blood." Master's thesis, 2015. http://hdl.handle.net/1822/41435.

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Dissertação de mestrado em Bioengenharia<br>Nosocomial infections are a worldwide concern due to their impact on patients’ health and costs to the health care system. The majority of these infections are associated with the use of indwelling medical devices, which serve as scaffold for biofilm formation by bacteria or other microorganisms. Due to its remarkable capability to form biofilms on medical devices, Staphylococcus epidermidis, a commensal bacterium of healthy human skin and mucosae, has emerged as one of major causes of medical devices-associated infections, being particularly associa
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21

Gonçalves, Vanessa Acúrcio da Silva. "Survival of Staphylococcus epidermidis biofilm-released cells in human blood and plasma." Master's thesis, 2016. http://hdl.handle.net/1822/44776.

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Dissertação de mestrado em Bioengineering<br>Staphylococcus epidermidis colonizes healthy human skin and mucosa as a commensal microbe. It is an opportunistic pathogen, since it requires a major breach in the host innate defence. Also, this bacterial species has become one of the leading nosocomial pathogens, in particular medical devices-related infections such as intravascular catheters. Accordingly, S. epidermidis causes at least 22 % of bloodstream infections, detected in intensive care in the United Sates. The main factor that often sustains the commensal lifestyle of this bacteria i
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Wermser, Charlotte. "Morphology, regulation and interstrain interactions in a new macrocolony biofilm model of the human pathogen \(Staphylococcus\) \(aureus\)." Doctoral thesis, 2019. https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-165931.

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The role of multicellularity as the predominant microbial lifestyle has been affirmed by studies on the genetic regulation of biofilms and the conditions driving their formation. Biofilms are of prime importance for the pathology of chronic infections of the opportunistic human pathogen Staphylococcus aureus. The recent development of a macrocolony biofilm model in S. aureus opened new opportunities to study evolution and physiological specialization in biofilm communities in this organism. In the macrocolony biofilm model, bacteria form complex aggregates with a sophisticated spatial organiza
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El-ezmerli, Nasreen Farouk. "Effect of nicotine on biofilm formation of streptococous mutans isolates from smoking versus non-smoking human subjects." Thesis, 2017. https://doi.org/10.7912/C2X94F.

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Indiana University-Purdue University Indianapolis (IUPUI)<br>Tooth decay is a complex dieto-bacterial disease with an association of social, behavioral and biological factors. Streptococcus mutans plays a major role in tooth decay. This endogenous oral microorganism adheres to tooth surfaces and grows and develops into micro-communities that mature and form dental biofilm. Development of cariogenic biofilm is one of the major factors associated with the tooth decay process. The use of tobacco is considered a great risk factor for oral diseases. Several studies demonstrated the association of t
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Pitre, Mindy Christina. "Microbial biodeterioration of human skeletal material from Tell Leilan, Syria (2900 – 1900 BCE)." Phd thesis, 2011. http://hdl.handle.net/10048/1745.

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Human bone is considered one of the most direct and insightful sources of information on peoples of the past. As a result, curation protocols have been developed to ensure that the integrity of human skeletal collections is maintained. Although collections are generally considered safe when these protocols are followed, the results of this investigation show that the Tell Leilan skeletal collection from Syria (circa 2900 – 1900 BCE) was contaminated by microbial growth (also known as biodeterioration) during curation. This biodeterioration was evaluated by light microscopy (LM), by the applica
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Olwoch, Ian P. "The effect of Pectinex Ultra SP-L on bacterial biofilms and human cell cultures in vitro." Thesis, 2014. http://hdl.handle.net/2263/43212.

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Biofilms are surface-bound bacterial colonies that are held together by a self-produced extracellular polymeric matrix. They are highly resistant to antibiotics and host defence mechanisms, and are known to be the cause of persistent infections. Biofilm-degrading enzymes have been shown to prevent biofilm formation, remove mature biofilm, and enhance the efficacy of antibiotics. This study investigated the antibacterial and antibiofilm actions of the commercial enzyme Pectinex Ultra SP-L (Pectinex), alone and in combination with antibiotics, on standard and clinical cultures of Staphylococcus
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Dias, Carla Sofia Pereira. "Antimicrobial resistance, biofilm formation and cell invasion by bacteria isolated from wild animals: potential impact in animal and human health." Doctoral thesis, 2018. http://hdl.handle.net/10348/8633.

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Antibiotics were originally developed for the treatment of bacterial infectious diseases. However, antibiotic resistance thwarts the treatment of infectious diseases globally, representing one of the most serious public health problems. The emergence of increasing numbers of antibiotic-resistant pathogens, some of which resistant to multiple classes of antibiotics, has implications not only for veterinary patients but also humans. Additionally, during the last years, wildlife has also been an important source of bacterial infectious diseases transmissible to humans. Zoonoses with a wild animal
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Santos, Beatriz Alexandra Pinto dos. "Caracterização fenotípica de isolados de Staphylococcus epidermidis após interação com sangue humano." Master's thesis, 2019. http://hdl.handle.net/1822/64668.

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Dissertação de mestrado em Biotecnologia<br>As infeções associadas aos cuidados de saúde afetam muitos pacientes diariamente. Staphylococcus epidermidis é uma das espécies mais frequentemente associadas com o surgimento destas infeções, estando particularmente relacionada com o uso de dispositivos médicos invasivos, devido à sua capacidade para aderir a estes e formar biofilmes. O diagnóstico destas infeções é normalmente feito através de hemoculturas. No entanto, devido à natureza comensal de S. epidermidis, é muito frequente ocorrerem contaminações da amostra durante a colheita, resultando e
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Martel, Jan, and 馬奕安. "Mineral–Protein Nanoparticles and Biofilm-Like Structures with Biomimetic Properties: their Formation in Vitro and their Relevance to Ectopic Calcification in Humans." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/38281218134493768740.

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博士<br>長庚大學<br>生物醫學研究所<br>99<br>Background: Putative living microorganisms termed nanobacteria (NB) have been associated with various human diseases, including atherosclerosis, cancer, chronic kidney disease, and kidney stone formation. On the other hand, the nature of NB and the question whether these entities are actually involved in human diseases remain controversial issues. Specific Aims: The main objectives of the present project were to determine the nature of NB and the role of serum proteins and gamma-irradiated fetal bovine serum (γ-FBS) during NB formation. In addition, we investigat
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Σπηλιοπούλου, Αναστασία. "Επίδραση biofilm θετικών στελεχών S. epidermidis στην ανοσολογική απόκριση ανθρώπινων μονοπυρήνων και μελέτη των πολυσακχαριτών του εξωκυττάριου χώρου (matrix)". Thesis, 2012. http://hdl.handle.net/10889/5766.

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Ο S. epidermidis αποτελεί κύριο μέλος της χλωρίδας του δέρματος και των βλεννογόνων του ανθρώπου, ενώ συνιστά ένα από τα συχνότερα παθογόνα που προκαλούν νοσοκομειακές λοιμώξεις, ιδιαίτερα σε ανοσοκατασταλμένους ή ασθενείς φέροντες προσθετικά υλικά. Κύριος λοιμογόνος παράγοντας του S. epidermidis είναι ο σχηματισμός βιομεμβράνης. Διάφοροι πολυσακχαρίτες έχουν απομονωθεί από την εξωκυττάρια ουσία του S. epidermidis και έχουν συσχετισθεί με το σχηματισμό βιομεμβράνης καθώς και με την παθογόνο δράση τους. Ο πλέον μελετημένος και εδραιωμένος είναι ο ΡΙΑ, ενώ οι άλλοι πολυσακχαρίτες (PS/A ή PNSG ή
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Sunil, Kumar B. "Developmental Strategies to Address Prosthetic Infection and Magneto-Responsive Biomaterials for Orthopaedic Applications." Thesis, 2015. http://etd.iisc.ernet.in/2005/3548.

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The issue of prosthetic infection leading to implant failure due to the formation of bacterial biofilms on biomaterial surfaces has been widely recognized as a major issue, often leading to revision surgery. The growing number of patients requiring synthetic biomaterials as implants is on the rise and so is the risk of infection arising from pre/peri-/post-operative surgical procedures. Traditional antibiotic treatment has led to the emergence of bacterial drug resistance. Therefore, the development of novel bactericidal methods to combat drug resistant microbial pathogens is the need of the h
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