Relevant bibliographies by topics / Human embryonic stem cell

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Human embryonic stem cell'

Author: Grafiati
Published: 4 June 2021
Last updated: 10 March 2023

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Journal articles on the topic "Human embryonic stem cell"

1

Pera, M. F., B. Reubinoff, and A. Trounson. "Human embryonic stem cells." Journal of Cell Science 113, no. 1 (2000): 5–10. http://dx.doi.org/10.1242/jcs.113.1.5.

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Embryonic stem (ES) cells are cells derived from the early embryo that can be propagated indefinitely in the primitive undifferentiated state while remaining pluripotent; they share these properties with embryonic germ (EG) cells. Candidate ES and EG cell lines from the human blastocyst and embryonic gonad can differentiate into multiple types of somatic cell. The phenotype of the blastocyst-derived cell lines is very similar to that of monkey ES cells and pluripotent human embryonal carcinoma cells, but differs from that of mouse ES cells or the human germ-cell-derived stem cells. Although our understanding of the control of growth and differentiation of human ES cells is quite limited, it is clear that the development of these cell lines will have a widespread impact on biomedical research.
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Challa, Stalin Reddy, and Swathi Goli. "Differentiation of Human Embryonic Stem Cells into Engrafting Myogenic Precursor Cells." Stem cell Research and Therapeutics International 1, no. 1 (2019): 01–05. http://dx.doi.org/10.31579/2643-1912/002.

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Degenerative muscle diseases affect muscle tissue integrity and function. Human embryonic stem cells (hESC) are an attractive source of cells to use in regenerative therapies due to their unlimited capacity to divide and ability to specialize into a wide variety of cell types. A practical way to derive therapeutic myogenic stem cells from hESC is lacking. In this study, we demonstrate the development of two serum-free conditions to direct the differentiation of hESC towards a myogenic precursor state. Using TGFß and PI3Kinase inhibitors in combination with bFGF we showed that one week of differentiation is sufficient for hESC to specialize into PAX3+/PAX7+ myogenic precursor cells. These cells also possess the capacity to further differentiate in vitro into more specialized myogenic cells that express MYOD, Myogenin, Desmin and MYHC, and showed engraftment in vivo upon transplantation in immunodeficient mice. Ex vivo myomechanical studies of dystrophic mouse hindlimb muscle showed functional improvement one month post-transplantation. In summary, this study describes a promising system to derive engrafting muscle precursor cells solely using chemical substances in serum-free conditions and without genetic manipulation.
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Shannon, Thomas A. "Human Embryonic Stem Cell Therapy." Theological Studies 62, no. 4 (2001): 811–24. http://dx.doi.org/10.1177/004056390106200407.

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Hoffman, Lisa M., and Melissa K. Carpenter. "Human Embryonic Stem Cell Stability." Stem Cell Reviews 1, no. 2 (2005): 139–44. http://dx.doi.org/10.1385/scr:1:2:139.

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Jones, Jeffrey M., and James A. Thomson. "Human Embryonic Stem Cell Technology." Seminars in Reproductive Medicine 18, no. 02 (2000): 219–24. http://dx.doi.org/10.1055/s-2000-12560.

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MORRISSEY, SUSAN R. "HUMAN EMBRYONIC STEM CELL DEBATE." Chemical & Engineering News Archive 82, no. 44 (2004): 22–23. http://dx.doi.org/10.1021/cen-v082n044.p022.

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Chan, E. M., and G. Q. Daley. "Human embryonic stem cell protocols." Bone Marrow Transplantation 38, no. 1 (2006): 79. http://dx.doi.org/10.1038/sj.bmt.1705407.

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Chen, Yifei, and Dongmei Lai. "Pluripotent States of Human Embryonic Stem Cells." Cellular Reprogramming 17, no. 1 (2015): 1–6. http://dx.doi.org/10.1089/cell.2014.0061.

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Thomson, James A., and Jon S. Odorico. "Human embryonic stem cell and embryonic germ cell lines." Trends in Biotechnology 18, no. 2 (2000): 53–57. http://dx.doi.org/10.1016/s0167-7799(99)01410-9.

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Gerecht-Nir, Sharon, and Joseph Itskovitz-Eldor. "Cell therapy using human embryonic stem cells." Transplant Immunology 12, no. 3-4 (2004): 203–9. http://dx.doi.org/10.1016/j.trim.2003.12.013.

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Dissertations / Theses on the topic "Human embryonic stem cell"

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Gertow, Karin. "Human embryonic stem cells : a novel model system for early human development /." Stockholm, 2006. http://diss.kib.ki.se/2006/91-7140-749-9/.

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Shah, Nadia Nisa. "Human embryonic stem cells : prospects for pancreatic β-cell differentiation". Thesis, University of Manchester, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.495052.

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The focus of this thesis was to explore different strategies in trying to generate putative pancreatic β-cells using one of the initial Wisconsin H7 hES cell lines. Prior to this, human pancreas development was assessed during the first trimester of pregnancy in an attempt to determine the spatial and temporal expression of development and mature pancreatic β-cell markers during this period. Spontaneous differentiation of hES cells can be induced by the formation of embryoid bodies (EBs) in suspension culture. EBs began to express markers of pancreatic β-cell development and function at a molecular, protein and functional level upon differentiation over a 3-week period. The constitutive over-expression of the terminal β-cell marker PAX4 enhances this process, whereas karyotypic abnormalities induced in hES cells over prolonged culture can hinder differentiation potential towards pancreatic β-cells. Directed differentiation strategies which mimic mouse pancreas development have led to the elucidation of an in vitro protocol to generate putative definitive endoderm from hES cells through the application of Wnt3a and Activin A in the presence of low serum. Indirect co-culture of this H7 hES cell-derived putative definitive endoderm with mouse islets did not lead to the differentiation of fully functional pancreatic β-cells. The hES cell-derived putative definitive endoderm did however influence the aging mouse islets in a positive manner by allowing the maintenance of insulin secretagogue-induced functional responses which are usually lost in culture. This may prove useful in maintaining viability of human islets during culture to be used for transportation therapies.
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Lin, Wenyu. "Investigating the immunomodulatory properties of human embryonic stem cell-derived mesenchymal stem cells." Thesis, Imperial College London, 2010. http://hdl.handle.net/10044/1/7060.

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The immunosuppressive property of mesenchymal stem cells (MSC) has been utilised to ameliorate autoimmune reactions such as graft-versus-host disease. However, variation exists in primary MSC isolated due to differences in donor age and tissue of origin. Alternatively, human embryonic stem cells (hESC) can be differentiated to homogeneous populations of MSC (hESCMSC), thus providing an unlimited source of MSC for cell therapy. In this study, the immunomodulatory properties of two hESC-MSC lines, hESC-MSC1 and hESC-MSC2, were compared with adult bone marrow-derived MSC (BM-MSC) and neonatal foreskin fibroblast (Fb). hESC-MSC were able to suppress the proliferation of anti-CD3/CD28-stimulated CD4+ T cells in contact and transwell systems. The immunosuppression was demonstrated by both the carboxyfluorescein diacetate succinimidyl ester (CFSE) and [3H]- thymidine proliferation assays. However, hESC-MSC were less potent and twice the number of adherent hESC-MSC (as measured by IC50) compared to BM-MSC and Fb were required to suppress T cell proliferation by 50%. Supernatants collected from transwells of MSC or Fb with T cells were shown to suppress T cell proliferation, suggesting that suppressive factors were only produced in the presence of activated T cells. Among several candidates, endothelial monocyte-activating polypeptide-II (EMAP-II) was identified as a potential suppressive factor. T cells also induced indoleamine-2,3- dioxygenase (IDO) expression in MSC and Fb. IDO led to the depletion of tryptophan, an essential amino acid, and/or the production of tryptophan metabolites (kynurenines), thereby inhibiting T cell proliferation. Interestingly, blocking of IDO with 1-methyltrytophan reversed the suppressive effect, implicating IDO as a potential mediator in T cell suppression. Concomitantly, several candidate suppressive factors in the supernatants have also been identified using antibody arrays. However, their functions require validation. In conclusion, hESC-MSC share similar suppressive properties as BM-MSC and represent a potential cell source for clinical purposes.
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Bigdeli, Narmin. "Derivation, characterization and differentiation of feeder-free human embryonic stem cells /." Göteborg : Department of Clinical Chemistry and Transfusion Medicine, Institute of Biomedicine at Sahlgrenska Academy, University of Gothenburg, 2010. http://hdl.handle.net/2077/22353.

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Faial, Caldas Macedo Amaral Tiago. "The role of BRACHYURY in human embryonic stem cell differentiation." Thesis, University of Cambridge, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.610541.

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Alawadhi, Aseel. "Human embryonic stem cell research : shaping regulations in Kuwait." Thesis, University of Aberdeen, 2016. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=231070.

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VanOudenhove, Jennifer J. "Mechanisms Regulating Early Mesendodermal Differentiation of Human Embryonic Stem Cells: A Dissertation." eScholarship@UMMS, 2016. http://escholarship.umassmed.edu/gsbs_diss/849.

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Key regulatory events take place at very early stages of human embryonic stem cell (hESC) differentiation to accommodate their ability to differentiate into different lineages; this work examines two separate regulatory events. To investigate precise mechanisms that link alterations in the cell cycle and early differentiation, we examined the initial stages of mesendodermal lineage commitment and observed a cell cycle pause that occurred concurrently with an increase in genes that regulate the G2/M transition, including WEE1. Inhibition of WEE1 prevented the G2 pause. Directed differentiation of hESCs revealed that cells paused during commitment to the endo- and mesodermal, but not ectodermal, lineages. Functionally, WEE1 inhibition during meso- and endodermal differentiation selectively decreased expression of definitive endodermal markers SOX17 and FOXA2. These findings reveal a novel G2 cell cycle pause required for endodermal differentiation. A role for phenotypic transcription factors in very early differentiation is unknown. From a screen of candidate factors during early mesendodermal differentiation, we found that RUNX1 is selectively and transiently up-regulated. Transcriptome and functional analyses upon RUNX1 depletion established a role for RUNX1 in promoting cell motility. In parallel, we discovered a loss of repression for several epithelial genes, indicating that RUNX1 knockdown impaired an epithelial to mesenchymal transition during differentiation. Cell biological and biochemical approaches revealed that RUNX1 depletion compromised TGFβ2 signaling. Both the decrease in motility and deregulated epithelial marker expression upon RUNX1 depletion were rescued by reintroduction of TGFβ2, but not TGFβ1. These findings identify novel roles for RUNX1-TGFβ2 signaling in mesendodermal lineage commitment.
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Alfaro, Alfonzo Antonio Alejandro. "Metabolomics study of human embryonic stem cell culture media." Thesis, University of Nottingham, 2015. http://eprints.nottingham.ac.uk/28850/.

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Self-renewal and pluripotency, the hallmarks of human embryonic stem cells (hESC), confer these cells with the capacity to expand indefinitely while maintaining the ability to differentiate into any cell type of the human body; thus, making hESC a valuable source of functional differentiated cells suitable for applications in regenerative medicine, drug discovery, biotechnology, biopharmaceuticals and developmental biology. However, the large-scale production of clinical-grade hESC, required for such applications, has been hampered by the current culture conditions in which hESC still depend on the use of mouse embryonic fibroblast-conditioned medium (MEF-CM) for their efficient growth. Therefore, investigation of the factors provided by MEFs is of the utmost importance to discover which components of MEF-CM allow the long-term expansion of undifferentiated hESC. While considerable progress has been made on the identification of the protein components of MEF-CM, very little is known about the small molecules (metabolites) secreted by MEFs. In this context, an untargeted metabolomics method was developed for the investigation of potential bioactive metabolites present in MEF-CM implicated in the proliferation and/or maintenance of pluripotency of hESC in vitro. A metabolomics method was applied and successfully identified a number of metabolites which were later confirmed in their identities with the use of authentic standards, to be further investigated for their effect on hESC culture. Interestingly, the addition of PGE2, 6-keto-PGF1α, 9, 12, 13-TriHOME, 7-Ketocholesterol and stearidonic acid (the metabolites found in MEF-CM) to the unconditioned medium (UM), a medium incapable of the maintenance of hESC, showed a delay in apoptosis when compared to the negative control UM; thus, suggesting that these metabolites could help with the proliferation of hESC. Increasing evidence that hESC secrete factors into their microenvironment that can also help them to proliferate or to maintain an undifferentiated state prompted the application of the same metabolomics method to the analysis of hESC spent culture media. The results identified lysophospholipids (LPLs) as potential molecules mediating some biological activities; however, the precise role of these LPLs still remains to be determined. Overall, the results of this thesis are expected to impact and add knowledge to the field of stem cell biology providing useful information for the creation and development of more efficient and defined culture conditions for the propagation of hESC with the appropriate quality to realise their widespread application in clinic and other research areas.
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Alaqel, Abdullah. "The directed differentiation of human embryonic stem cells to lung cell lineages." Thesis, University of Bath, 2017. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.760955.

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Human embryonic stem cells (hESCs) show significant therapeutic potential in treating degenerative disorders. This is in part because of their ability to produce a limitless supply of starting cells and their potential to differentiate into more than 200 different cell types. The aim of the current research was to generate a robust stage wise protocol for the differentiation of hESCs to respiratory epithelial cells. The epithelial cells could then be used either for transplantation studies or, as an in vitro model for drug toxicity testing. In order to achieve this goal, we must identify the key steps in lung development and apply these to the differentiation protocol. In this study, we maintained Shef3 hESCs in their undifferentiated pluripotent state to expand the cells prior to the differentiated towards the definitive endoderm (DE) lineage. I used a two-stage protocol based on culture with a novel glycogen synthase kinase-3 (GSK-3) inhibitor (termed 1m), along with Activin-A. We confirmed the status of the cells by a combination of immunostaining and PCR. We showed loss of the pluripotency markers (Sox2 and Oct3/4) and gain of DE markers (Sox17, FoxA2 and CXCR4). After the induction of DE from hESCs, we then treated the cells with transforming growth factor (TGF)-β and bone morphogenetic protein (BMP) pathway inhibitors (SB431542 and Noggin respectively). This combinatorial treatment resulted in the differentiation into the anterior foregut endoderm (AFE) lineage based on expression of Pax9 and FoxA2 plus the up-regulation of Sox2. Further differentiation of AFE derivatives into more mature epithelial cells, termed lung progenitor cells (LPCs), was achieved following the treatment of AFE cells with a cocktail of trophic factors (BMP4, EGF, bFGF, FGF10, KGF and Wnt3a) yielded a population of NKX2.1-positive and FoxA2-positive cells that potentially corresponded to the lung lineage. Finally, prolonged treatment with FGF10 and FGF2 on LPC derived hESCs induced proximal (CC10, MUC5AC) and distal (SPB, SPC) airway epithelial cells. In addition, we also utilised the ectopic expression of an adenovirus expressing NKX2.1 to promote lung maturation. In conclusion, we have generated a protocol for the differentiation of hESCs into mature lung-like cells. The generation of these cells in vitro could potentially lead to a better in vitro model for toxicity testing and the development of novel therapies for promoting regeneration of lungs in patients with severe lung disorders.
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Ericson, Robin J. "Bridging solutions to the religion and science conflict over human embryonic stem cell research." Fairfax, VA : George Mason University, 2007. http://hdl.handle.net/1920/2926.

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Thesis (Ph. D.)--George Mason University, 2007.<br>Title from PDF t.p. (viewed Jan. 17, 2008). Thesis director: Richard E. Rubenstein. Submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Conflict Analysis and Resolution. Vita: p. 228. Includes bibliographical references (p. 222-227). Also available in print.
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Books on the topic "Human embryonic stem cell"

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Human embryonic stem cell protocols. 2nd ed. Humana Press, 2010.

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Kursad, Turksen, ed. Human embryonic stem cell protocols. Humana Press, 2006.

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Kursad, Turksen. Human Embryonic Stem Cell Protocols. Humana Press, 2006. http://dx.doi.org/10.1385/1597450464.

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Turksen, Kursad, ed. Human Embryonic Stem Cell Protocols. Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-2668-8.

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Turksen, Kursad, ed. Human Embryonic Stem Cell Protocols. Humana Press, 2010. http://dx.doi.org/10.1007/978-1-60761-369-5.

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Dr, Sullivan Stephen, Eggan Kevin, and Cowan Chad, eds. Human embryonic stem cells: The practical handbook. John Wiley & Sons, 2007.

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S, Odorico Jon, Zhang S. -C, and Pedersen Roger A, eds. Human embryonic stem cells. Garland Science/BIOS Scientific Publishers, 2006.

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Chiu, Arlene, and Mahendra S. Rao. Human Embryonic Stem Cells. Humana Press, 2003. http://dx.doi.org/10.1385/1592594239.

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Sullivan, Stephen, Chad A. Cowan, and Kevin Eggan, eds. Human Embryonic Stem Cells. John Wiley & Sons, Ltd, 2007. http://dx.doi.org/10.1002/9780470511619.

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Chiu, Arlene Y., and Mahendra S. Rao, eds. Human Embryonic Stem Cells. Humana Press, 2003. http://dx.doi.org/10.1007/978-1-59259-423-8.

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Book chapters on the topic "Human embryonic stem cell"

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Xu, Chunhui, and Melissa K. Carpenter. "Human Embryonic Stem Cell-Derived Cardiomyocytes." In Human Embryonic Stem Cells. Humana Press, 2003. http://dx.doi.org/10.1007/978-1-59259-423-8_10.

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Hertsenberg, Andrew J., and James L. Funderburgh. "Generation of Corneal Keratocytes from Human Embryonic Stem Cells." In Embryonic Stem Cell Protocols. Springer New York, 2015. http://dx.doi.org/10.1007/7651_2015_231.

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Guloglu, M. Oktar, and Anna Larsen. "Dopaminergic Differentiation of Human Embryonic Stem Cells on PA6-Derived Adipocytes." In Embryonic Stem Cell Protocols. Springer New York, 2015. http://dx.doi.org/10.1007/7651_2015_235.

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Renz, Peter F., and Tobias A. Beyer. "A Concise Protocol for siRNA-Mediated Gene Suppression in Human Embryonic Stem Cells." In Embryonic Stem Cell Protocols. Springer New York, 2015. http://dx.doi.org/10.1007/7651_2015_212.

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Roubal, Ivan, Sun Joo Park, and Yong Kim. "Derivation of Neural Precursor Cells from Human Embryonic Stem Cells for DNA Methylomic Analysis." In Embryonic Stem Cell Protocols. Springer New York, 2014. http://dx.doi.org/10.1007/7651_2014_152.

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Radan, Lida, Chris S. Hughes, Jonathan H. Teichroeb, Lynne-Marie Postovit, and Dean H. Betts. "Delivering Antisense Morpholino Oligonucleotides to Target Telomerase Splice Variants in Human Embryonic Stem Cells." In Embryonic Stem Cell Protocols. Springer New York, 2015. http://dx.doi.org/10.1007/7651_2015_226.

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Avery, John, and Stephen Dalton. "Methods for Derivation of Multipotent Neural Crest Cells Derived from Human Pluripotent Stem Cells." In Embryonic Stem Cell Protocols. Springer New York, 2015. http://dx.doi.org/10.1007/7651_2015_234.

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Reier, Paul J., John Q. Trojanowski, Virginia M.-Y. Lee, and Margaret J. Velardo. "Studies of a Human Neuron-Like Cell Line in Stroke and Spinal Cord Injury." In Human Embryonic Stem Cells. Humana Press, 2003. http://dx.doi.org/10.1007/978-1-59259-423-8_18.

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Downing, Gregory J. "A Researcher’s Guide to Federally Funded Human Embryonic Stem Cell Research in the United States." In Human Embryonic Stem Cells. Humana Press, 2003. http://dx.doi.org/10.1007/978-1-59259-423-8_2.

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Zwaka, Thomas P. "Genetic Modification of Human Embryonic Stem Cells." In Human Cell Culture. Springer Netherlands, 2007. http://dx.doi.org/10.1007/978-1-4020-5983-4_4.

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Conference papers on the topic "Human embryonic stem cell"

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Guan, Benjamin X., Bir Bhanu, Prudence Talbot, and Sabrina Lin. "Automated Human Embryonic Stem Cell Detection." In 2012 IEEE Second International Conference on Healthcare Informatics, Imaging and Systems Biology (HISB). IEEE, 2012. http://dx.doi.org/10.1109/hisb.2012.25.

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Chen, Weiqiang, Luis G. Villa-Diaz, Yubing Sun, et al. "Nanotopography Directs Fate of Human Embryonic Stem Cells." In ASME 2012 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/sbc2012-80222.

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Human embryonic stem cells (hESCs) have remarkable potentials for breakthroughs in future cell-based therapeutics owing to their self-renewal capability and pluripotency [1–2]. However, their intrinsic mechanosensitivity to biophysical signals from the local cellular microenvironment is not well characterized [3–4]. In this work, we introduced a simple, yet precise, microfabrication strategy for accurate control and patterning of local nanoroughness on glass surfaces using photolithography and reactive ion etching (RIE). Our results demonstrated that nanoscale topological features could provide a potent regulatory signal over a diverse array of hESC behaviors, including their morphology, adhesion, proliferation and clonal expansion, and differentiation.
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Singh, Ankur, Shalu Suri, Ted T. Lee, et al. "Adhesive Signature-Based, Label-Free Isolation of Human Pluripotent Stem Cells." In ASME 2012 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/sbc2012-80044.

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Generation of human induced pluripotent stem cells (hiPSCs) from fibroblasts and other somatic cells represents a highly promising strategy to produce auto- and allo-genic cell sources for therapeutic approaches as well as novel models of human development and disease1. Reprogramming protocols involve transduction of the Yamanaka factors Oct3/4, Sox2, Klf4, and c-Myc into the parental somatic cells, followed by culturing the transduced cells on mouse embryonic fibroblast (MEF) or human fibroblast feeder layers, and subsequent mechanical dissociation of pluripotent cell-like colonies for propagation on feeder layers1, 2. The presence of residual parental and feeder-layer cells introduces experimental variability, pathogenic contamination, and promotes immunogenicity3. Similar to human embryonic stem cells (hESCs), reprogrammed hiPSCs suffer from the unavoidable problem of spontaneous differentiation due to sub-optimal feeder cultures4, growth factors5, and the feeder-free substrate6. Spontaneously differentiated (SD)-hiPSCs display reduced pluripotency and often contaminate hiPSC cultures, resulting in overgrowth of cultures and compromising the quality of residual pluripotent stem cells5. Therefore, the ability to rapidly and efficiently isolate undifferentiated hiPSCs from the parental somatic cells, feeder-layer cells, and spontaneously differentiated cells is a crucial step that remains a bottleneck in all human pluripotent stem cell research.
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Li, X., L. Zhao, Steve K. W. Oh, et al. "Measurement of human embryonic stem cell in the growing cycle." In Ninth International Symposium on Laser Metrology, edited by Chenggen Quan and Anand Asundi. SPIE, 2008. http://dx.doi.org/10.1117/12.814615.

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Melvin, Tracy, Nicolas Perney, Peter Horak, Neil Hanley, and James Hughes. "Optical Classification of Human Embryonic Stem Cells." In Optical Trapping Applications. OSA, 2013. http://dx.doi.org/10.1364/ota.2013.tt1d.6.

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Zhang, Shaohong, Hai Huang, Jiqiao Li, Baoying Zeng, Wenxiao Qiu, and Dongqing Xie. "Putative protein interaction analysis for human embryonic stem cell derived cardiomyocytes." In 2016 9th International Congress on Image and Signal Processing, BioMedical Engineering and Informatics (CISP-BMEI). IEEE, 2016. http://dx.doi.org/10.1109/cisp-bmei.2016.7853016.

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Korin, Natanel, Avishay Bransky, Uri Dinnar, and Shulamit Levenberg. "Modeling and Studying Human Embryonic Stem Cell Culture Conditions in Pulsed Flow Micro-Reactors." In ASME 2008 9th Biennial Conference on Engineering Systems Design and Analysis. ASMEDC, 2008. http://dx.doi.org/10.1115/esda2008-59168.

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Embryonic stem (ES) cells research is a promising field for tissue engineering due to their proliferative capacity and differentiation abilities. The culture of Human Embryonic Stem Cells (hESC) in microchannel bioreactors can be valuable for hESC cell biology studies and hESC tissue engineering applications. We have previously demonstrated the long-term culture of mammalian (HFF-Human Foreskin Fibroblasts) cells in a microchannel (130μm) bioreactor under constant perfusion in a simple approach. However, hESC were found to be highly sensitive to flow and did not grow under flow rates which were proper for HFF long-term culture. Here, we propose the use of a novel automated periodic perfusion system to co-culture hESC with HFF in a microchannel bioreactor. The method is based on short temporal pulsed flows of medium renewal followed by long static incubation periods. The short pulsed exposure to shear enables shear sensitive cells (e.g., hESC) to withstand the medium flow. The present work studies experimentally and via numerical simulations the conditions required for hESC culture in a microchannel bioreactor using the periodic perfusion method. Conventional soft-lithography techniques were used to fabricate PDMS microchannels (100 μm) sealed reversibly with glass cover slides. HESC were seeded in the microchannel with HFF, incubated for several hours and then connected to a perfusion system which contained: a syringe pump, a permeable tube oxygenator, and waste container. The ability of the periodic perfusion protocols to prevent hESC de-attachment and maintain their culture was examined. Mass transport and fluid mechanics models were used to evaluate the culture conditions within the micro-bioreactor (shear stress, oxygen level, nutritious etc.). 3D finite element mass transport analysis (Comsol 3.3) was preformed to examine the oxygen levels in the microchannel as a function of time and design parameters. Altogether, the experimental results and the theoretical model indicate that the use of a periodic perfusion bioreactor is a suitable and promising method to culture hESC in a microreactor. Culturing undifferentiated human ES cell colonies in a micro-bioreactor is an initial step toward utilizing microfluidic techniques to investigate embryonic stem cell biology.
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Guan, Benjamin Xueqi, Bir Bhanu, Ninad Thakoor, Prudence Talbot, and Sabrina Lin. "Human Embryonic Stem Cell Detection by Spatial Information and Mixture of Gaussians." In 2011 IEEE International Conference on Healthcare Informatics, Imaging and Systems Biology (HISB). IEEE, 2011. http://dx.doi.org/10.1109/hisb.2011.30.

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Abdelalim, Essam, Bushra Memon, Manale Karam та Sara Al-Khawaga. "Enhancement of differentiation of multipotent pancreatic β cell precursors from human embryonic stem cells". У Qatar Foundation Annual Research Conference Proceedings. Hamad bin Khalifa University Press (HBKU Press), 2018. http://dx.doi.org/10.5339/qfarc.2018.hbpp297.

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Novikov, S. M., J. Beermann, S. I. Bozhevolnyi, L. M. Harkness, and M. Kassem. "Raman microscopy of individual living human embryonic stem cells." In SPIE Photonics Europe, edited by Jürgen Popp, Wolfgang Drexler, Valery V. Tuchin, and Dennis L. Matthews. SPIE, 2010. http://dx.doi.org/10.1117/12.854207.

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Reports on the topic "Human embryonic stem cell"

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Zuckerman, Kenneth S. Reparative Medicine: Production of Erythrocytes & Platelets from Human Embryonic Stem Cells. Defense Technical Information Center, 2012. http://dx.doi.org/10.21236/ada566171.

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Gupta, Shweta. The Revolution of Human Organoids in Cell Biology. Natur Library, 2020. http://dx.doi.org/10.47496/nl.blog.12.

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Organoids are a new research tool derived from human pluripotent or adult stem cells or somatic cells in vitro to form small, self-organizing 3-dimensional structures that simulate many of the functions of native organs
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Griend, Donald Vander. Isolation and Growth of Prostate Stem Cells and Establishing Cancer Cell Lines from Human Prostate Tumors. Defense Technical Information Center, 2009. http://dx.doi.org/10.21236/ada511041.

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Pailino, Lia, Lihua Lou, Alberto Sesena Rubfiaro, Jin He, and Arvind Agarwal. Nanomechanical Properties of Engineered Cardiomyocytes Under Electrical Stimulation. Florida International University, 2021. http://dx.doi.org/10.25148/mmeurs.009775.

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Engineered cardiomyocytes made of human-induced pluripotent stem cells (iPSC) present phenotypical characteristics similar to human fetal cardiomyocytes. There are different factors that are essential for engineered cardiomyocytes to be functional, one of them being that their mechanical properties must mimic those of adult cardiomyocytes. Techniques, such as electrical stimulation, have been used to improve the extracellular matrix's alignment and organization and improve the intracellular environment. Therefore, electrical stimulation could potentially be used to enhance the mechanical properties of engineered cardiac tissue. The goal of this study is to establish the effects of electrical stimulation on the elastic modulus of engineered cardiac tissue. Nanoindentation tests were performed on engineered cardiomyocyte constructs under seven days of electrical stimulation and engineered cardiomyocyte constructs without electrical stimulation. The tests were conducted using BioSoft™ In-Situ Indenter through displacement control mode with a 50 µm conospherical diamond fluid cell probe. The Hertzian fit model was used to analyze the data and obtain the elastic modulus for each construct. This study demonstrated that electrically stimulated cardiomyocytes (6.98 ± 0.04 kPa) present higher elastic modulus than cardiomyocytes without electrical stimulation (4.96 ± 0.29 kPa) at day 7 of maturation. These results confirm that electrical stimulation improves the maturation of cardiomyocytes. Through this study, an efficient nanoindentation method is demonstrated for engineered cardiomyocyte tissues, capable of capturing the nanomechanical differences between electrically stimulated and non-electrically stimulated cardiomyocytes.
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