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Dissertations / Theses on the topic 'Human histology'

Author: Grafiati
Published: 4 June 2021
Last updated: 1 February 2022

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1

Asaad, Kamil. "Comparative histology of human skin." Thesis, University of Bradford, 2010. http://hdl.handle.net/10454/5711.

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There are 5 distinct aspects to this study. (i) Two histological stains for collagen were compared against each other for the first time, namely Herovici's technique and picrosirius-polarization. (ii) Skin samples from embalmed cadaveric tissue from human cadavers were compared against samples taken from surgical patients. (iii) Skin samples were studied from different regions of the body to assess if dermal structure correlates with scarring potential. (iv) Skin samples were sectioned in a plane parallel to the epidermis to gain further insight into dermal structure. (v) A novel basement membrane stain was produced. Type I and type III collagen are important structural constituents of dermis and play a crucial role in wound healing. Only two traditional histological methods are thought to differentiate between them, so avoiding the need for antibodies. These were compared against each other for the first time in order to establish differences in image quality and discrimination between Type I and type III collagen. Neither technique requires antibodies, however picrosirius requires polarisation microscopy. to result in a clearer, consistently reproducible collagen staining pattern than the picrosirius method and more importantly did not require elaborate apparatus to analyze. Additionally other cellular elements were visible. Skin samples for research are often obtained from surgical excision. This clearly limits which tissues are available for comparative study to those areas operated on. Studying samples from embalmed medical school cadavers has the great advantage of studying areas of the body not routinely available from common surgical procedures. It was therefore desirable to assess whether embalmed cadaveric tissues exhibited different properties by virtue of their age and the embalming process compared to fresh surgical specimens, in order to give confidence that studies utilising the former would be equally valid. To test this, 58 skin samples from embalmed medical school cadavers were compared to skin samples from 38 fresh operative specimens. The levels of tissue preservation and processing artefacts were similar in both groups. Embalmed medical school cadavers clearly offer an opportunity to study tissue areas not routinely available during surgery. This is the first time such a comparison has been made. Many things will affect the final appearance of the scar, but the single most important determinant is the body region affected. The most common areas for unfavourable scarring, specifically keloid or hypertrophic scarring have been shown to be the ear, deltoid and sternal areas. To test the hypothesis that there is no difference in histological structure of skin that correlates to body region, comparative histology was undertaken exploring the regional variations of skin characteristics in 58 cadaveric samples. Closely comparable samples were taken from the deltoid (9), abdomen (13), sternum (10), post-auricular (5), earlobe (12) and eyelid (9). Epidermal thickness, epidermal appendage density and collagen fibre orientation were examined and qualitative structural differences were assessed for each region Skin samples were then grouped by both topographical location of the body and scarring potential. Skin samples exhibited qualitative and quantifiable regional variations in the characteristics studied. Epidermal thickness and appendage counts did not correlate with scarring potential. Both however were statistically significantly higher in skin sampled from the head compared to the trunk. Bundles of collagen fibres in the reticular dermis were grouped according to their orientation in relation to the coronal plane; either parallel, oblique or perpendicular. The ratio of oblique to parallel fibres was statistically significantly higher in body areas with poorer scarring prognosis. This corresponds to a more disorganised arrangement of collagen fibres in these areas. Further qualitative understanding of dermal collagen fibres came from perpendicular to conventional histological samples. This new method stained basement membranes purple, cytoplasm was stained greenish-brown and nuclei dark brown. Collagen fibres were either thin and blue or thick and green. This method was compared to PAS staining and although required more preparative steps allows greater identification of other cellular structures.
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2

Miszkiewicz, Justyna J. "Ancient human bone histology and behaviour." Thesis, University of Kent, 2014. https://kar.kent.ac.uk/38319/.

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3

Butterworth, Rosalind J. "The histology of human granulating wounds." Thesis, University of Leicester, 1992. http://hdl.handle.net/2381/34293.

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1. The prime aim of this study was to investigate the structure and cellularity of human granulation tissue, by the taking of punch biopsies from clinical wounds, a method not previously reported in wound research. It was intended both to further the understanding of wound biology, and to provide baseline information for clinical trials of therapeutic agents, such as dressing materials and growth factors. 2. To evaluate the validity of the universally used animal models of wound healing, and the extent to which data from them can be extrapolated to the human wound. 3. To relate histological criteria to the progress of wound healing as observed clinically, in both normally healing and unhealthy wounds. This might allow diagnosis of difficult healing problems to be made by biopsy, and also has relevance to wound biology in providing circumstantial evidence of cell function. 4. To compare conventional and immunocytochemical staining techniques in identification of cell types in human granulation tissue. 5. As a particular area of interest, to clarify the role of the myofibroblast in wound contraction by identification of a suitable marker for this cell in human granulation tissue, and by examining the temporal relationship between myofibroblast presence and wound contraction.
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4

Nor, Faridah Mohd. "A comparative microscopic study of human and non-human long bone histology." Thesis, University of Bradford, 2009. http://hdl.handle.net/10454/4463.

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Identification of human or nonhuman skeletal remains is important in assisting the police and law enforcement officers for the investigation of forensic cases. Identification of bone can be difficult, especially in fragmented remains. It has been reported that 25 to 30% of medicolegal cases, which involved nonhuman skeletal remains have been mistaken for human. In such cases, histomorphometric method was used to identify human and nonhuman skeletal remains. However, literature has shown that histomorphometric data for human and nonhuman bone were insufficient. Additionally, age estimation in bone may help in the identification of human individual, which can be done by using a histomorphometric method. Age estimation is based on bone remodeling process, where microstructural parameters have strong correlations with age. Literature showed that age estimation has been done on the American and European populations. However, little work has been done in the Asian population. The aims of this project were thus, to identify human and nonhuman bone, and to estimate age in human bones by using histomorphometric analysis. In this project, 64 human bones and 65 animal bones were collected from the mortuary of the Universiti Kebangsaan Malaysia Medical Centre and the Zoos in Malaysia, respectively. A standard bone preparation was used to prepare human and nonhuman bone thin sections for histomorphometric assessment. Assessments were made on the microstructural parameters such as cortical thickness, medullary cavity diameter, osteon count, osteon diameter, osteon area, osteon perimeter, Haversian canal diameter, Haversian canal area, Haversian canal perimeter, and Haversian lamella count per osteon by using image analysis, and viewed under a transmitted light microscope. The microstructural measurements showed significant differences between human and nonhuman samples. The discriminant functions showed correct classification rates for 81.4% of cases, and the accuracy of identification was 96.9% for human and 66.2% for animal. Human age estimation showed a standard error of estimate of 10.41 years, comparable with those in the literature. This study project offers distinct advantages over currently available histomorphometric methods for human and nonhuman identification and human age estimation. This will have significant implications in the assessment of fragmentary skeletal and forensic population samples for identification purposes.
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5

Nor, Faridah M. "A comparative microscopic study of human and non-human long bone histology." Thesis, University of Bradford, 2009. http://hdl.handle.net/10454/4463.

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Abstract:
Identification of human or nonhuman skeletal remains is important in assisting the police and law enforcement officers for the investigation of forensic cases. Identification of bone can be difficult, especially in fragmented remains. It has been reported that 25 to 30% of medicolegal cases, which involved nonhuman skeletal remains have been mistaken for human. In such cases, histomorphometric method was used to identify human and nonhuman skeletal remains. However, literature has shown that histomorphometric data for human and nonhuman bone were insufficient. Additionally, age estimation in bone may help in the identification of human individual, which can be done by using a histomorphometric method. Age estimation is based on bone remodeling process, where microstructural parameters have strong correlations with age. Literature showed that age estimation has been done on the American and European populations. However, little work has been done in the Asian population. The aims of this project were thus, to identify human and nonhuman bone, and to estimate age in human bones by using histomorphometric analysis. In this project, 64 human bones and 65 animal bones were collected from the mortuary of the Universiti Kebangsaan Malaysia Medical Centre and the Zoos in Malaysia, respectively. A standard bone preparation was used to prepare human and nonhuman bone thin sections for histomorphometric assessment. Assessments were made on the microstructural parameters such as cortical thickness, medullary cavity diameter, osteon count, osteon diameter, osteon area, osteon perimeter, Haversian canal diameter, Haversian canal area, Haversian canal perimeter, and Haversian lamella count per osteon by using image analysis, and viewed under a transmitted light microscope. The microstructural measurements showed significant differences between human and nonhuman samples. The discriminant functions showed correct classification rates for 81.4% of cases, and the accuracy of identification was 96.9% for human and 66.2% for animal. Human age estimation showed a standard error of estimate of 10.41 years, comparable with those in the literature. This study project offers distinct advantages over currently available histomorphometric methods for human and nonhuman identification and human age estimation. This will have significant implications in the assessment of fragmentary skeletal and forensic population samples for identification purposes.
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6

Axon, Steven Robert. "Correlation of the hemodynamics and histology in the human right coronary artery." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp05/MQ62910.pdf.

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7

Barrett, Andrew W. "Immunological studies of human oral mucosal Langerhans cells." Thesis, University of Newcastle Upon Tyne, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.333511.

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8

McCulley, Michelle Caroline. "Classification and genetic analysis of human facial features." Thesis, University College London (University of London), 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.271409.

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9

Williams, P. H. "On the distribution of bumble bees : with particular regard to patterns within the British Isles." Thesis, University of Cambridge, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.355051.

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10

Newsholme, D. M. "Studies of selection in vitro for resistance to Leptosphaeria maculans (Desm.) Ces. and de Not. (stem canker) : Using secondarily embryonic cultures of Brassica napus L. ssp. oleifera (Metzg.) Sinsk (winter oilseed rape)." Thesis, University of Cambridge, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.372279.

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11

Duffen, J. "Dietry control of the salmonella carrier state in poultry." Thesis, University of Cambridge, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.355506.

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12

Matthews, H. R. "Electrophysiological studies of vertebrate rod photoreceptors." Thesis, University of Cambridge, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.373259.

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13

Saphier, D. J. "Electrophysiology and endocrine function of rat tuberoinfundibular neurones." Thesis, University of Cambridge, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.373694.

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14

Zhang, J. "A role for abscisic acid in root to shoot communication of changes in the soil environment." Thesis, Lancaster University, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.234647.

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15

Wyness, L. E. "Effects of water stress on the infectivity and nutrition of Erysiphe pisi, powdery mildew of pea." Thesis, Lancaster University, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.356335.

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16

Silva, D. L. R. de. "A critical assessment of the role of abscisic acid and calcium ions in regulating stomatal movements in Commelina communis L." Thesis, Lancaster University, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.373807.

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17

Wolfenden, J. "Nitrogen fixation associated with Spartina anglica : A study of nitrogen-fixing microorganisms and their contribution to the growth of a developing Spartina anglica marsh in the Lune Estuary." Thesis, Lancaster University, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.355042.

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18

Rowland, A. J. "Fluxes of nitrogen and carbon in barley exposed to NO2̲." Thesis, Lancaster University, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.375193.

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19

Battram, J. C. "Chloride balance in the brown trout Salmo trutta L. in neutral and acidified waters." Thesis, Lancaster University, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.373479.

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20

Wright, E. A. "The effects of sulphur dioxide and nitrogen dioxide, singly and in mixture, on the growth of birch trees." Thesis, Lancaster University, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.372938.

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21

Afzal, Faiza. "The role of nitric oxide in the control of osteoblast metabolism and maintenance of bone integrity." Thesis, Imperial College London, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.271576.

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22

Stevenson, Shirley Anne. "The plasma membrane cytoskeleton of the cardiac muscle cell : spatial organisation and interactions of component proteins." Thesis, Imperial College London, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.300484.

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23

Rutbemberwa, Alleluiah. "Characterisation of HIV-1-Specific CD8+t Lymphocyte immune responses in HIV-1 infected Ugandan individuals." Thesis, Imperial College London, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.289822.

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24

Leclerq, Pascale. "Quantitative post-mortem study of cortical interneurons in chronic schizophrenia." Thesis, Imperial College London, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.300445.

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25

Rossi, B. C. "Macrophage function in African trypanosomiasis." Thesis, Brunel University, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.373784.

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26

Birch, P. J. "The role of presynaptic receptors in the regulation of noradrenaline synthesis in rat hippocampus." Thesis, University of Oxford, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.370237.

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27

Kanowski, P. J. "Characterization and interpretation of variation in forest trees : A reappraisal based on a progeny test of Pinus caribaea Morelet." Thesis, University of Oxford, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.375259.

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28

Lee, S. H. "Studies on tissue macrophages and their properties in murine malaria." Thesis, University of Oxford, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.372083.

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29

Arthur, R. P. "In vitro functional studies of rat T helper cell heterogeneity." Thesis, University of Oxford, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.355759.

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30

Ellard, F. M. "Growth in symbiotic hydra." Thesis, University of Oxford, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.375255.

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31

Rowe, K. A. "Quantitative microscopic studies of normal and dystrophic chicken muscle." Thesis, University of Oxford, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.375318.

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32

Siva-Jothy, M. T. "Sperm competition in the odonata." Thesis, University of Oxford, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.370301.

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33

Price, D. J. "The organisation and development of area 18 of the cat's visual cortex." Thesis, University of Oxford, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.355793.

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34

Page, Karen Mary. "Mathematical models in embryology : the selection, regulation and speed of formation of patterns." Thesis, University of Oxford, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.302119.

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35

King, L. A. "Molecular biology of insect picornaviruses." Thesis, University of Oxford, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.370278.

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36

Niebuhr, V. "The coordination of behaviour between breeding herring gull mates." Thesis, University of Oxford, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.355792.

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37

Bucklow, C. W. M. "English science and the development of plant physiology in the second half of the seventeenth century." Thesis, University of Oxford, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.371615.

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38

Nerquaye-Tetteh, J. O. "Endometrial changes associated with implantation in the field vole, Microtus agrestis, and the bank vole, Clethrionomys glareolus." Thesis, University of Oxford, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.375270.

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39

Thomas, J. "The effects of homocysteine on potassium channel function in human platelets." Thesis, University of Oxford, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.270751.

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40

Ryan, E. N. "Studies on the role of calcium in the regulation of tyrosine hydroxylase in the rat brain." Thesis, University of Oxford, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.371575.

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41

Darsley, M. J. "Studies on the interaction of monoclonal antibodies with antigens of biological interest." Thesis, University of Oxford, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.354824.

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42

Hamad, A.-W. R. "Factors affecting nitrogen retention in lambs." Thesis, University of Oxford, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.371514.

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43

Staunton, D. "The coding and infection strategy of Thogoto virus." Thesis, University of Oxford, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.235153.

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44

Doncaster, C. P. "The spatial organisation of urban foxes (Vulpes vulpes) in Oxford." Thesis, University of Oxford, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.354822.

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45

Bell, K. M. "Pattern formation and growth during the development of the embryonic chick limb." Thesis, University of Oxford, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.355728.

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46

Ridout, C. J. "Biological control of Rhizoctonia solani on lettuce with species of Trichoderma." Thesis, University of Hull, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.376233.

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47

Koper, W. J. "An investigation of PGF2a induced functional luteolysis in the rat." Thesis, University of Hull, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.376376.

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48

Sherlock, L. A. "The properties and regulation of yeast amine oxidases." Thesis, University of Hull, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.383786.

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49

Kenny, N. "Studies related to steroidogenesis by cells isolated from the corpus luteum of the pseudopregnant rat." Thesis, University of Hull, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.376374.

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50

Walter, D. J. "Fibre metabolism in the rat." Thesis, University of Edinburgh, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.370595.

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