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Relevant bibliographies by topics / Human pluripotent stem cells (hPSC)

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Academic literature on the topic 'Human pluripotent stem cells (hPSC)'

Author: Grafiati

Published: 4 June 2021

Last updated: 8 February 2022

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Journal articles on the topic "Human pluripotent stem cells (hPSC)"

1

Hayashi, Yohei, and Miho Kusuda Furue. "Biological Effects of Culture Substrates on Human Pluripotent Stem Cells." Stem Cells International 2016 (2016): 1–11. http://dx.doi.org/10.1155/2016/5380560.

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In recent years, as human pluripotent stem cells (hPSCs) have been commonly cultured in feeder-free conditions, a number of cell culture substrates have been applied or developed. However, the functional roles of these substrates in maintaining hPSC self-renewal remain unclear. Here in this review, we summarize the types of these substrates and their effect on maintaining hPSC self-renewal. Endogenous extracellular matrix (ECM) protein expression has been shown to be crucial in maintaining hPSC self-renewal. These ECM molecules interact with integrin cell-surface receptors and transmit their c

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Maysubara, Hiroyuki, Akira Niwa, Tatsutoshi Nakahata, and Megumu K. Saito. "NK Cells from Human Pluripotent Stem Cells for Immunotherapy." Blood 132, Supplement 1 (2018): 4955. http://dx.doi.org/10.1182/blood-2018-99-115499.

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Abstract Natural Killer (NK) cells are a one of innate lymphocytes and show cytotoxicity against tumour cells without prior antigen specific stimulation. . NK cells can demonstrate stronger cytotoxicity than T cells in the absence of MHC Class I, and survive short lifespan from several weeks to one month. It suggested that NK cells show low risk of cytokine long-term secretion inside patient's body. Previous studies have developed peripheral blood mononuclear cells (PBMC) derived NK cells expansions or NK cells differentiation from cord blood (CB) cells for immunotherapy. Expansion trial using

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Wei, Yanxing, Tianyu Wang, Lishi Ma, et al. "Efficient derivation of human trophoblast stem cells from primed pluripotent stem cells." Science Advances 7, no. 33 (2021): eabf4416. http://dx.doi.org/10.1126/sciadv.abf4416.

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Human trophoblast stem cells (hTSCs) provide a valuable model to study placental development and function. While primary hTSCs have been derived from embryos/early placenta, and transdifferentiated hTSCs from naïve human pluripotent stem cells (hPSCs), the generation of hTSCs from primed PSCs is problematic. We report the successful generation of TSCs from primed hPSCs and show that BMP4 substantially enhances this process. TSCs derived from primed hPSCs are similar to blastocyst-derived hTSCs in terms of morphology, proliferation, differentiation potential, and gene expression. We define the

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Lipsitz, Yonatan Y., Curtis Woodford, Ting Yin, Jacob H. Hanna, and Peter W. Zandstra. "Modulating cell state to enhance suspension expansion of human pluripotent stem cells." Proceedings of the National Academy of Sciences 115, no. 25 (2018): 6369–74. http://dx.doi.org/10.1073/pnas.1714099115.

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The development of cell-based therapies to replace missing or damaged tissues within the body or generate cells with a unique biological activity requires a reliable and accessible source of cells. Human pluripotent stem cells (hPSC) have emerged as a strong candidate cell source capable of extended propagation in vitro and differentiation to clinically relevant cell types. However, the application of hPSC in cell-based therapies requires overcoming yield limitations in large-scale hPSC manufacturing. We explored methods to convert hPSC to alternative states of pluripotency with advantageous b

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Lim, Seakcheng, Rachel A. Shparberg, Jens R. Coorssen, and Michael D. O’Connor. "Application of the RBBP9 Serine Hydrolase Inhibitor, ML114, Decouples Human Pluripotent Stem Cell Proliferation and Differentiation." International Journal of Molecular Sciences 21, no. 23 (2020): 8983. http://dx.doi.org/10.3390/ijms21238983.

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Retinoblastoma binding protein 9 (RBBP9) is required for maintaining the expression of both pluripotency and cell cycle genes in human pluripotent stem cells (hPSCs). An siRNA-based study from our group showed it does so by influencing cell cycle progression through the RB/E2F pathway. In non-pluripotent cells, RBBP9 is also known to have serine hydrolase (SH) activity, acting on currently undefined target proteins. The role of RBBP9 SH activity in hPSCs, and during normal development, is currently unknown. To begin assessing whether RBBP9 SH activity might contribute to hPSC maintenance, hPSC

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Ran, Dan, Wei-Jong Shia, Miao-Chia Lo, et al. "RUNX1a enhances hematopoietic lineage commitment from human embryonic stem cells and inducible pluripotent stem cells." Blood 121, no. 15 (2013): 2882–90. http://dx.doi.org/10.1182/blood-2012-08-451641.

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Abstract Advancements in human pluripotent stem cell (hPSC) research have potential to revolutionize therapeutic transplantation. It has been demonstrated that transcription factors may play key roles in regulating maintenance, expansion, and differentiation of hPSCs. In addition to its regulatory functions in hematopoiesis and blood-related disorders, the transcription factor RUNX1 is also required for the formation of definitive blood stem cells. In this study, we demonstrated that expression of endogenous RUNX1a, an isoform of RUNX1, parallels with lineage commitment and hematopoietic emerg

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Nemade, Harshal, Aviseka Acharya, Umesh Chaudhari, et al. "Cyclooxygenases Inhibitors Efficiently Induce Cardiomyogenesis in Human Pluripotent Stem Cells." Cells 9, no. 3 (2020): 554. http://dx.doi.org/10.3390/cells9030554.

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Application of human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) is limited by the challenges in their efficient differentiation. Recently, the Wingless (Wnt) signaling pathway has emerged as the key regulator of cardiomyogenesis. In this study, we evaluated the effects of cyclooxygenase inhibitors on cardiac differentiation of hPSCs. Cardiac differentiation was performed by adherent monolayer based method using 4 hPSC lines (HES3, H9, IMR90, and ES4SKIN). The efficiency of cardiac differentiation was evaluated by flow cytometry and RT-qPCR. Generated hPSC-CMs were characterised us

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Karam, Manale, Ihab Younis, Noor R. Elareer, Sara Nasser, and Essam M. Abdelalim. "Scalable Generation of Mesenchymal Stem Cells and Adipocytes from Human Pluripotent Stem Cells." Cells 9, no. 3 (2020): 710. http://dx.doi.org/10.3390/cells9030710.

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Human pluripotent stem cells (hPSCs) can provide unlimited supply for mesenchymal stem cells (MSCs) and adipocytes that can be used for therapeutic applications. Here we developed a simple and highly efficient all-trans-retinoic acid (RA)-based method for generating an off-the-shelf and scalable number of human pluripotent stem cell (hPSC)-derived MSCs with enhanced adipogenic potential. We showed that short exposure of multiple hPSC lines (hESCs/hiPSCs) to 10 μM RA dramatically enhances embryoid body (EB) formation through regulation of genes activating signaling pathways associated with cell

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Walasek, Marta A., Crystal Chau, Christian Barborini, et al. "A Reproducible and Simple Method to Generate Red Blood Cells from Human Pluripotent Stem Cells." Blood 134, Supplement_1 (2019): 1189. http://dx.doi.org/10.1182/blood-2019-128830.

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Erythroid cells generated from human pluripotent stem cells (hPSCs) can potentially offer an unlimited and safe supply of red blood cells (RBCs) for transfusion. Human PSC-derived erythroid cells at various stages of differentiation can also be used to model blood diseases, test new drug candidates, and develop cellular and genetic therapies. Although several protocols for deriving RBCs from hPSCs have been described, these are typically complex, involving multiple culture steps that may include co-culture with feeder cells, and exhibit large variability in erythroid cell yields between hPSC l

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Rehakova, Daniela, Tereza Souralova, and Irena Koutna. "Clinical-Grade Human Pluripotent Stem Cells for Cell Therapy: Characterization Strategy." International Journal of Molecular Sciences 21, no. 7 (2020): 2435. http://dx.doi.org/10.3390/ijms21072435.

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Human pluripotent stem cells have the potential to change the way in which human diseases are cured. Clinical-grade human embryonic stem cells and human induced pluripotent stem cells have to be created according to current good manufacturing practices and regulations. Quality and safety must be of the highest importance when humans’ lives are at stake. With the rising number of clinical trials, there is a need for a consensus on hPSCs characterization. Here, we summarize mandatory and ′for information only′ characterization methods with release criteria for the establishment of clinical-grade

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Dissertations / Theses on the topic "Human pluripotent stem cells (hPSC)"

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Bargehr, Johannes. "The role of human embryonic stem cell-derived epicardium in myocardial graft development." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/276112.

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Takeuchi, Hiroki. "Endodermal differentiation of human pluripotent stem cells to insulin-producing cells in 3D culture." Kyoto University, 2014. http://hdl.handle.net/2433/189668.

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Vargas, Valderrama Alejandra. "Différenciation des cellules souches pluripotentes humaines en cellules endothéliales et cellules hématopoïétiques via une population du type hémangioblastique Efficient hPSC differentiation into endothelial and hematopoietic cells via a hemangioblast-like population." Thesis, université Paris-Saclay, 2020. http://www.theses.fr/2020UPASQ021.

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Les cellules endothéliales (CE) et les cellules hématopoïétiques dérivées des cellules souches pluripotentes humaines (CSPh) sont un outil prometteur pour l'ingénierie tissulaire, la thérapie cellulaire et pour la découverte de nouveaux médicaments. Les CE assurent la formation des vaisseaux sanguins, tandis que les cellules hématopoïétiques matures sont impliquées dans le transport de l'oxygène, l'hémostase et la réponse immunitaire entre autres. Dans cette étude, nous avons différencié des CSPh en une population bipotente de type hémangioblastique pour générer des populations pures de ces de

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Kardel, Melanie Dawn. "Analysis of hematopoiesis from human pluripotent stem cells." Thesis, University of British Columbia, 2011. http://hdl.handle.net/2429/33333.

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Human embryonic stem (ES) or induced pluripotent stem (iPS) cells have the potential ability to generate all of the cell types in the body. If their differentiation into relevant cell types of interest can be effectively controlled, they are attractive for developmental studies, disease modelling, drug testing, and advancing regenerative medicine. The generation of hematopoietic cells from human ES/iPS cells has been reported, but is highly variable and often inefficient. My specific objective in this thesis was to more fully characterize the process whereby hematopoietic cells are generated f

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Adams, William James. "Human Vascular Endothelium from Induced Pluripotent Stem Cells." Thesis, Harvard University, 2013. http://dissertations.umi.com/gsas.harvard:10816.

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The vascular endothelium is a dynamic cellular interface that displays a unique phenotypic plasticity. This plasticity is critical for vascular function and when dysregulated is pathogenic in several diseases. The development of new human endothelial genotype-phenotype studies, personalized vascular medicine efforts and cell based regenerative therapies are limited by the unavailability of patient-specific endothelial cells. Induced pluripotent stem cells (iPSC) offer great promise as a new personalized source of endothelium; however, the reproducibility, fidelity and functionality of iPSC-der

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Hariharan, Krithika. "In vitro nephrogenesis from human pluripotent stem cells." Doctoral thesis, Humboldt-Universität zu Berlin, 2018. http://dx.doi.org/10.18452/19194.

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Die Homöostase wird maßgeblich durch die Niere, bestehend aus Millionen funktioneller Untereinheiten, den Nephronen, aufrechtherhalten. Chronisch geschädigte Nephrone führen zur Entwicklung einer terminalen Nierenerkrankung (TNE). Die Erzeugung renaler Zellen aus humanen pluripotenten Stammzellen (hPSCs) stellt eine vielversprechende Strategie zur regenerativen Therapie und Behandlung von TNE dar. In der vorliegenden Arbeit wurde ein Protokoll zur Differenzierung von renalen Vorläufern (RV) aus hPSCs entwickelt, welches nephronale Zelltypen und Strukturen in vitro und ex vivo erzeugte. Eine se

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Henry, Marianne Patricia. "The genomic health of human pluripotent stem cells." Thesis, Brunel University, 2018. http://bura.brunel.ac.uk/handle/2438/17081.

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Human pluripotent stem cells are increasingly used for cell-based regenerative therapies worldwide, with the use of embryonic and induced pluripotent stem cells as potential treatments for a range of debilitating and chronic conditions. However, with the level of chromosomal aneuploidies the cells may generate in culture, their safety for therapeutic use could be in question. This study aimed to develop sensitive and high-throughput assays for the detection and quantification of human pluripotent stem cell aneuploidies, to assess any changes in their positioning in nuclei, as well as investiga

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Dawud, Raed Abu. "Studies of DPPA4 in human pluripotent stem cells." Thesis, University of Sheffield, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.486782.

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Pluripotent human embryonic stem cells (hES) can proliferate indefinitely in vitro and differentiate in all three germ layers and represent therefore a valuable tool for drug discovery, cell replacement therapy and regenerative medicine. Therefore, it is fundamental to understand the genetic network that regulates' pluripotency. A microarray experiment carried out in our laboratory compared the expression profile of undifferentiated versus differentiated hES cells. The results highlighted the down regulation upon differentiation of both already known master genes of pluripotency, like OCT3/4 a

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Morishima, Tatsuya. "Neutrophil differentiation from human-induced pluripotent stem cells." Kyoto University, 2011. http://hdl.handle.net/2433/151911.

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Golebiewska, Anna. "Epigenetic changes in differentiation of human pluripotent stem cells." Thesis, University of Newcastle Upon Tyne, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.500923.

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The molecular mechanisms underlying pluripotency and lineage specification from embryonic stem cells (ESCs) are still largely unclear. A great deal of recent research has concentrated on the epigenetic basis of pluripotency of ESCs and studies have also suggested that the differences between various cell types may be due to differences in global epigenetic profiles. Understanding the epigenetic differences between pluripotent cells, such as human ESCs and embryonal carcinoma (EC) cells, and their differentiated counterparts may allow further understanding of the role epigenetics plays in devel

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Books on the topic "Human pluripotent stem cells (hPSC)"

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Schwartz, Philip H., and Robin L. Wesselschmidt, eds. Human Pluripotent Stem Cells. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-201-4.

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Lakshmipathy, Uma, Chad C. MacArthur, Mahalakshmi Sridharan, and Rene H. Quintanilla. Human Pluripotent Stem Cells. John Wiley & Sons, Inc., 2018. http://dx.doi.org/10.1002/9781119394372.

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Amit, Michal, and Joseph Itskovitz-Eldor, eds. Atlas of Human Pluripotent Stem Cells. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-548-0.

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Human pluripotent stem cells: Methods and protocols. Humana, 2011.

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Ye, Kaiming, and Sha Jin, eds. Human Embryonic and Induced Pluripotent Stem Cells. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-267-0.

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Cheng, Tao, ed. Hematopoietic Differentiation of Human Pluripotent Stem Cells. Springer Netherlands, 2015. http://dx.doi.org/10.1007/978-94-017-7312-6.

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Amit, M., and Joseph Itskovitz-Eldor. Atlas of human pluripotent stem cells: Derivation and culturing. Humana Press, 2012.

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Healy, Lyn, and Ludmila Ruban. Atlas of Human Pluripotent Stem Cells in Culture. Springer US, 2015. http://dx.doi.org/10.1007/978-1-4899-7507-2.

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Sha, Jin, and SpringerLink (Online service), eds. Human Embryonic and Induced Pluripotent Stem Cells: Lineage-Specific Differentiation Protocols. Springer Science+Business Media, LLC, 2012.

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President's Council on Bioethics (U.S.). Alternative sources of human pluripotent stem cells: A white paper of the President's Council on Bioethics. President's Council on Bioethics, 2005.

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Book chapters on the topic "Human pluripotent stem cells (hPSC)"

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Brafman, David A. "Generation, Expansion, and Differentiation of Human Pluripotent Stem Cell (hPSC) Derived Neural Progenitor Cells (NPCs)." In Methods in Molecular Biology. Springer New York, 2014. http://dx.doi.org/10.1007/7651_2014_90.

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Kong, Chi-Wing, Lin Geng, and Ronald A. Li. "High-Content Electrophysiological Analysis of Human Pluripotent Stem Cell-Derived Cardiomyocytes (hPSC-CMs)." In Methods in Molecular Biology. Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7553-2_12.

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Hu, Ruiqi, Xiaoting Zhu, and Nan Yang. "Direct Differentiation of Functional Neurons from Human Pluripotent Stem Cells (hPSCs)." In Methods in Molecular Biology. Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1601-7_8.

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Cutts, Josh, Nicholas Brookhouser, and David A. Brafman. "Generation of Regionally Specific Neural Progenitor Cells (NPCs) and Neurons from Human Pluripotent Stem Cells (hPSCs)." In Methods in Molecular Biology. Springer New York, 2016. http://dx.doi.org/10.1007/7651_2016_357.

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Lidgerwood, Grace E., Alex W. Hewitt, Alice Pébay, and Damián Hernández. "The Use of Human Pluripotent Stem Cells (hPSCs) and CRISPR-Mediated Gene Editing in Retinal Diseases." In Essentials in Ophthalmology. Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-15-9184-6_31.

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Healy, Lyn, and Ludmila Ruban. "Passaging Pluripotent Stem Cells." In Atlas of Human Pluripotent Stem Cells in Culture. Springer US, 2014. http://dx.doi.org/10.1007/978-1-4899-7507-2_7.

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Chen, Gina Y., and Jiang F. Zhong. "Pluripotent Human Stem Cells: An Overview." In Stem Cells and Cancer Stem Cells, Volume 1. Springer Netherlands, 2011. http://dx.doi.org/10.1007/978-94-007-1709-1_1.

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Lumelsky, Nadya. "Pancreatic Differentiation of Pluripotent Stem Cells." In Human Embryonic Stem Cells. Humana Press, 2003. http://dx.doi.org/10.1007/978-1-59259-423-8_9.

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Hoffman, Robert M. "Hair Follicle Pluripotent Stem (hfPS) Cells." In Human Adult Stem Cells. Springer Netherlands, 2009. http://dx.doi.org/10.1007/978-90-481-2269-1_8.

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Usdin, Steve. "Ethical Issues Associated with Pluripotent Stem Cells." In Human Embryonic Stem Cells. Humana Press, 2003. http://dx.doi.org/10.1007/978-1-59259-423-8_1.

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Conference papers on the topic "Human pluripotent stem cells (hPSC)"

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Bockman, Matthew D., Igor I. Katkov, Stephen B. Jones, Vsevolod Katkov, and Ilya Yakhnenko. "ComfortFreezer™: A Benchtop LN2–Free Programmable Freezer for Cryopreservation of Adherent Cells in Multi-Well Plates for Cell-Based High Content Screening." In ASME 2012 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/imece2012-85469.

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Human pluripotent stem cells (hPSCs) and their progeny such as hPSC-derived cardiomyocytes and neural cells hold great potential as a source for cell therapy and regenerative medicine, as well can be effectively used for high high content screening (HCS) of drug candidates and for toxicity tests. Cryopreservation (CP), storage, and shipment of the cells are key elements for eventual clinical, pharmaceutical and environmental applications, which will require large numbers of quality controlled and ready for use cells. Traditionally, the cells are frozen in suspensions of either fully dissociate

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Hookway, Tracy A., Doan Nguyen, Chunhui Xu, Mary B. Wagner, and Todd C. McDevitt. "Engineering cardiospheres from human pluripotent stem cells." In 2014 40th Annual Northeast Bioengineering Conference (NEBEC). IEEE, 2014. http://dx.doi.org/10.1109/nebec.2014.6972817.

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Tachizaki, Takehiro, Reiko Sakaguchi, Shiho Terada, Ken-ichiro Kamei, and Hideki Hirori. "Response of human induced pluripotent stem cells to terahertz radiation." In CLEO: Applications and Technology. OSA, 2021. http://dx.doi.org/10.1364/cleo_at.2021.jm4f.4.

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Burgess, C., X. Varelas, E. E. Morrisey, and D. N. Kotton. "Generation of Alveolar Epithelial Type I Cells from Human Pluripotent Stem Cells." In American Thoracic Society 2020 International Conference, May 15-20, 2020 - Philadelphia, PA. American Thoracic Society, 2020. http://dx.doi.org/10.1164/ajrccm-conference.2020.201.1_meetingabstracts.a2311.

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Singh, Ankur, Shalu Suri, Ted T. Lee, et al. "Adhesive Signature-Based, Label-Free Isolation of Human Pluripotent Stem Cells." In ASME 2012 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/sbc2012-80044.

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Generation of human induced pluripotent stem cells (hiPSCs) from fibroblasts and other somatic cells represents a highly promising strategy to produce auto- and allo-genic cell sources for therapeutic approaches as well as novel models of human development and disease1. Reprogramming protocols involve transduction of the Yamanaka factors Oct3/4, Sox2, Klf4, and c-Myc into the parental somatic cells, followed by culturing the transduced cells on mouse embryonic fibroblast (MEF) or human fibroblast feeder layers, and subsequent mechanical dissociation of pluripotent cell-like colonies for propag

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Li, Y., K. C. Goldfarbmuren, C. Rios, and M. A. Seibold. "Robust and Highly Specific Generation of Human Airway Epithelial Basal Stem Cells from Induced Pluripotent Stem Cells." In American Thoracic Society 2020 International Conference, May 15-20, 2020 - Philadelphia, PA. American Thoracic Society, 2020. http://dx.doi.org/10.1164/ajrccm-conference.2020.201.1_meetingabstracts.a2314.

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Geti, Imbisaat. "Derivation of Hepatocyte Like Cells from Human Pluripotent Stem Cells in GMP Compliant Conditions." In Qatar Foundation Annual Research Conference Proceedings. Hamad bin Khalifa University Press (HBKU Press), 2016. http://dx.doi.org/10.5339/qfarc.2016.hbop2136.

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Iberite, F., M. Salerno, C. Canale, A. Rosa, and L. Ricotti. "Influence of substrate stiffness on human induced pluripotent stem cells: preliminary results*." In 2019 41st Annual International Conference of the IEEE Engineering in Medicine & Biology Society (EMBC). IEEE, 2019. http://dx.doi.org/10.1109/embc.2019.8857397.

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Afify, Said M., Ghmkin Hassan, Akimasa Seno, Yoshiaki Iwasaki, and Masaharu Seno. "Abstract PO-094: Human pluripotent stem cells acquire malignancy under tumor microenvironment." In Abstracts: AACR Virtual Special Conference on Tumor Heterogeneity: From Single Cells to Clinical Impact; September 17-18, 2020. American Association for Cancer Research, 2020. http://dx.doi.org/10.1158/1538-7445.tumhet2020-po-094.

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Cuevas Ocana, Sara, Amy Wong, Magomet Aushev, et al. "How can gene-editing of human pluripotent stem cells help cystic fibrosis?" In ERS International Congress 2019 abstracts. European Respiratory Society, 2019. http://dx.doi.org/10.1183/13993003.congress-2019.oa2125.

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Reports on the topic "Human pluripotent stem cells (hPSC)"

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Ying, Mingyao. Modeling Aggressive Medulloblastoma Using Human-Induced Pluripotent Stem Cells. Defense Technical Information Center, 2015. http://dx.doi.org/10.21236/ada620932.

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Setaluri, Vijayasaradhi. Differentiation of Neonatal Human-Induced Pluripotent Stem Cells to Prostate Epithelial Cells: A Model to Study Prostate Cancer Development. Defense Technical Information Center, 2013. http://dx.doi.org/10.21236/ada583418.

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Setaluri, Vijayasaradhi. Differentiation of Neonatal Human-Induced Pluripotent Stem Cells to Prostate Epithelial Cells: A Model to Study Prostate Cancer Development. Defense Technical Information Center, 2014. http://dx.doi.org/10.21236/ada609443.

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Gupta, Shweta. The Revolution of Human Organoids in Cell Biology. Natur Library, 2020. http://dx.doi.org/10.47496/nl.blog.12.

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Organoids are a new research tool derived from human pluripotent or adult stem cells or somatic cells in vitro to form small, self-organizing 3-dimensional structures that simulate many of the functions of native organs

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