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Dissertations / Theses on the topic 'Human pluripotent stem cells (hPSC)'

Author: Grafiati

Published: 4 June 2021

Last updated: 8 February 2022

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1

Bargehr, Johannes. "The role of human embryonic stem cell-derived epicardium in myocardial graft development." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/276112.

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2

Takeuchi, Hiroki. "Endodermal differentiation of human pluripotent stem cells to insulin-producing cells in 3D culture." Kyoto University, 2014. http://hdl.handle.net/2433/189668.

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3

Vargas, Valderrama Alejandra. "Différenciation des cellules souches pluripotentes humaines en cellules endothéliales et cellules hématopoïétiques via une population du type hémangioblastique Efficient hPSC differentiation into endothelial and hematopoietic cells via a hemangioblast-like population." Thesis, université Paris-Saclay, 2020. http://www.theses.fr/2020UPASQ021.

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Les cellules endothéliales (CE) et les cellules hématopoïétiques dérivées des cellules souches pluripotentes humaines (CSPh) sont un outil prometteur pour l'ingénierie tissulaire, la thérapie cellulaire et pour la découverte de nouveaux médicaments. Les CE assurent la formation des vaisseaux sanguins, tandis que les cellules hématopoïétiques matures sont impliquées dans le transport de l'oxygène, l'hémostase et la réponse immunitaire entre autres. Dans cette étude, nous avons différencié des CSPh en une population bipotente de type hémangioblastique pour générer des populations pures de ces de

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4

Kardel, Melanie Dawn. "Analysis of hematopoiesis from human pluripotent stem cells." Thesis, University of British Columbia, 2011. http://hdl.handle.net/2429/33333.

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Human embryonic stem (ES) or induced pluripotent stem (iPS) cells have the potential ability to generate all of the cell types in the body. If their differentiation into relevant cell types of interest can be effectively controlled, they are attractive for developmental studies, disease modelling, drug testing, and advancing regenerative medicine. The generation of hematopoietic cells from human ES/iPS cells has been reported, but is highly variable and often inefficient. My specific objective in this thesis was to more fully characterize the process whereby hematopoietic cells are generated f

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5

Adams, William James. "Human Vascular Endothelium from Induced Pluripotent Stem Cells." Thesis, Harvard University, 2013. http://dissertations.umi.com/gsas.harvard:10816.

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The vascular endothelium is a dynamic cellular interface that displays a unique phenotypic plasticity. This plasticity is critical for vascular function and when dysregulated is pathogenic in several diseases. The development of new human endothelial genotype-phenotype studies, personalized vascular medicine efforts and cell based regenerative therapies are limited by the unavailability of patient-specific endothelial cells. Induced pluripotent stem cells (iPSC) offer great promise as a new personalized source of endothelium; however, the reproducibility, fidelity and functionality of iPSC-der

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6

Hariharan, Krithika. "In vitro nephrogenesis from human pluripotent stem cells." Doctoral thesis, Humboldt-Universität zu Berlin, 2018. http://dx.doi.org/10.18452/19194.

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Die Homöostase wird maßgeblich durch die Niere, bestehend aus Millionen funktioneller Untereinheiten, den Nephronen, aufrechtherhalten. Chronisch geschädigte Nephrone führen zur Entwicklung einer terminalen Nierenerkrankung (TNE). Die Erzeugung renaler Zellen aus humanen pluripotenten Stammzellen (hPSCs) stellt eine vielversprechende Strategie zur regenerativen Therapie und Behandlung von TNE dar. In der vorliegenden Arbeit wurde ein Protokoll zur Differenzierung von renalen Vorläufern (RV) aus hPSCs entwickelt, welches nephronale Zelltypen und Strukturen in vitro und ex vivo erzeugte. Eine se

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7

Henry, Marianne Patricia. "The genomic health of human pluripotent stem cells." Thesis, Brunel University, 2018. http://bura.brunel.ac.uk/handle/2438/17081.

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Human pluripotent stem cells are increasingly used for cell-based regenerative therapies worldwide, with the use of embryonic and induced pluripotent stem cells as potential treatments for a range of debilitating and chronic conditions. However, with the level of chromosomal aneuploidies the cells may generate in culture, their safety for therapeutic use could be in question. This study aimed to develop sensitive and high-throughput assays for the detection and quantification of human pluripotent stem cell aneuploidies, to assess any changes in their positioning in nuclei, as well as investiga

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8

Dawud, Raed Abu. "Studies of DPPA4 in human pluripotent stem cells." Thesis, University of Sheffield, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.486782.

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Pluripotent human embryonic stem cells (hES) can proliferate indefinitely in vitro and differentiate in all three germ layers and represent therefore a valuable tool for drug discovery, cell replacement therapy and regenerative medicine. Therefore, it is fundamental to understand the genetic network that regulates' pluripotency. A microarray experiment carried out in our laboratory compared the expression profile of undifferentiated versus differentiated hES cells. The results highlighted the down regulation upon differentiation of both already known master genes of pluripotency, like OCT3/4 a

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9

Morishima, Tatsuya. "Neutrophil differentiation from human-induced pluripotent stem cells." Kyoto University, 2011. http://hdl.handle.net/2433/151911.

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10

Golebiewska, Anna. "Epigenetic changes in differentiation of human pluripotent stem cells." Thesis, University of Newcastle Upon Tyne, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.500923.

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The molecular mechanisms underlying pluripotency and lineage specification from embryonic stem cells (ESCs) are still largely unclear. A great deal of recent research has concentrated on the epigenetic basis of pluripotency of ESCs and studies have also suggested that the differences between various cell types may be due to differences in global epigenetic profiles. Understanding the epigenetic differences between pluripotent cells, such as human ESCs and embryonal carcinoma (EC) cells, and their differentiated counterparts may allow further understanding of the role epigenetics plays in devel

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11

Yamanaka, Yoshihiro. "Recapitulating the human segmentation clock with pluripotent stem cells." Kyoto University, 2020. http://hdl.handle.net/2433/254519.

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12

Pinho, Sandra Isabel da Rocha Lourenco de. "Regulation of pluripotent states in human embryonic stem cells." Thesis, Imperial College London, 2009. http://hdl.handle.net/10044/1/5884.

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A growing array of mouse and human pluripotent stem cell lines has been derived from the early embryo as well as from adult cells reprogrammed by ectopic expression of transcription factors – i.e. induced pluripotent stem (iPS) cells. These cell lines share the expression of key pluripotency markers and are able to self-renew and to generate differentiated progenies when induced. Their relationship to each other and whether they correspond to different pluripotent states with distinct developmental capacities and affiliations in vivo remains unclear, however. Profiling chromatin in a particula

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13

Binti, Kamarudin Taty Anna. "Differentiation of human pluripotent stem cells into corneal epithelial like cells." Thesis, University of Newcastle upon Tyne, 2018. http://hdl.handle.net/10443/4182.

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Cornea is the clear outermost protective layer of the eye which enables transmission of light onto the retina. The corneal epithelium is regenerated by limbal stem cells (LSCs), whose loss/dysfunction results in limbal stem cell deficiency (LSCD). Transplantations of ex vivo expanded autologous LSCs from patient's healthy eye onto the affected eye have provided a successful treatment for unilateral LSCD. This however is not applicable to patient with total bilateral LSCD, whose both eyes are affected. This thesis investigated the potential of human induced-pluripotent stem cell (hiPSCs) to dif

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14

Vitillo, Loriana. "Focal adhesion kinase regulation of human embryonic stem cells." Thesis, University of Manchester, 2014. https://www.research.manchester.ac.uk/portal/en/theses/focal-adhesion-kinase-regulation-of-human-embryonic-stem-cells(31052725-50a4-4d34-a1eb-018be57986af).html.

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Undifferentiated human embryonic stem cells (hESCs) grow on the extracellular matrix (ECM) substrate fibronectin (FN) in defined feeder-free conditions. The ECM is part of the hESCs pluripotent niche and supports their maintenance, but the contribution to survival remains to be elucidated. Understanding the mechanism of survival is particularly crucial in hESCs, since it affects their expansion in cell culture and ultimately translation of research to the clinic. HESCs bind to FN mainly via alpha5β1- integrin, known to be upstream of important survival cascades in other cell types. However, it

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15

Rouhani, Foad Jafari. "Natural and modified variations in human induced pluripotent stem cells." Thesis, University of Cambridge, 2015. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.708541.

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16

Taura, Daisuke. "Induction and isolation of vascular cells from human induced pluripotent stem cells." Kyoto University, 2010. http://hdl.handle.net/2433/97941.

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17

Hirami, Yasuhiko. "Generation of retinal cells from mouse and human induced pluripotent stem cells." Kyoto University, 2010. http://hdl.handle.net/2433/97948.

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18

Qin, Jie [Verfasser]. "Enhancing differentiation of human induced pluripotent stem cells / Jie Qin." Aachen : Hochschulbibliothek der Rheinisch-Westfälischen Technischen Hochschule Aachen, 2014. http://d-nb.info/1066813477/34.

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19

Patani, Rickie. "Generating motor neuron subtype diversity from human pluripotent stem cells." Thesis, University of Cambridge, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.610349.

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20

Arango, Maria del Pilar Vazquez. "Factors controlling the pluripotent state of human embryonic stem cells." Thesis, University of Oxford, 2012. https://ora.ox.ac.uk/objects/uuid:754cefcc-97e7-4884-bf5d-3efcd9a377ae.

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In the field of regenerative medicine, human embryonic stem (hES) cells offer the potential to treat degenerative diseases and replace damaged or non-functional tissue. However, it is not fully clear how hES cells retain a pluripotent state or differentiate into specific cell lineages. Although most of previous research has found that various proteins are responsible for the balance in hES cell maintenance and differentiation, there is mounting evidence for non-coding RNAs as important players in this task. Data from our laboratory shows that there are genes that encode for variants of the non

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21

Noakes, Zoe. "Generation of functional striatal neurons from human pluripotent stem cells." Thesis, Cardiff University, 2016. http://orca.cf.ac.uk/98932/.

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The striatal neuronal populations comprise medium spiny projection neurons (MSNs) and GABAergic and cholinergic interneurons. Huntington’s disease (HD) involves massive degeneration of striatal neurons. The derivation of MSNs and interneurons from human pluripotent stem cells (hPSCs) would allow modelling of striatal function and HD in vitro, as well as provide a viable source of tissue for cell replacement therapy. Our lab has previously demonstrated that Activin A can induce MSN fate in hPSCs, and that these cells can survive and differentiate in vitro and in vivo. In this study, it was foun

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22

Omer, Attya. "Modeling human neural development and diseases using pluripotent stem cells." Thesis, Université Paris-Saclay (ComUE), 2017. http://www.theses.fr/2017SACLS589.

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La microcéphalie est une maladie neurologique du nouveau-né qui se traduit par une circonférence réduite de la tête, une déficience intellectuelle et des défauts anatomiques du cerveau. La microcéphalie peut être la conséquence d’une infection, de stress environnementaux ou de mutations génétiques.Le cerveau commence à se former dès la cinquième semaine de grossesse et est majoritairement constitué de cellules souches neuronales, cellules qui conservent une capacité a se reproduire a l’identique sans se spécialiser. Cette première phase de prolifération est importante pour générer suffisamment

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23

Arber, Charles. "Generation of disease-relevant neurons from human pluripotent stem cells." Thesis, Imperial College London, 2013. http://hdl.handle.net/10044/1/14670.

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This thesis describes investigations into exogenous factors that influence fate-choices during human pluripotent stem cell (PSC) differentiation in vitro. I describe novel growth factor environments and small molecule regimes that provide patterning signals enabling directed differentiation of human PSCs towards biomedically relevant neurons. I provide evidence that the TGFβ growth factor Activin can promote ventral telencephalic differentiation. Small adjustments in Activin administration can produce an over-representation of forebrain derived medium spiny neurons and cortical interneurons, w

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24

Buchrieser, Julian. "Understanding human mononuclear phagocyte ontogeny using human induced pluripotent stem cells (iPSCs)." Thesis, University of Oxford, 2016. https://ora.ox.ac.uk/objects/uuid:aaf18203-5f30-4d6a-8f51-3096b29af252.

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Tissue-resident macrophages (MΦ) such as microglia, Kupffer and Langerhans cells derive from Myb-independent yolk sac (YS) progenitors generated before the emergence of hematopoietic stem cells (HSCs). Myb-independent YS-derived resident MΦ self-renew locally, independently of circulating adult monocytes and HSCs. In contrast, adult blood monocytes as well as infiltrating, gut and dermal MΦ derive from Myb-dependent HSCs and are less proliferative. These findings are derived from the mouse, using gene knock-outs and lineage tracing, but their applicability to human development has

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25

Li, Sen, and 李森. "Calcium signaling in human pluripotent stem cell-derived ventricular cardiomyocytes." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2014. http://hdl.handle.net/10722/208028.

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Human pluripotent stem cells (hPSCs) serve as a potential unlimited ex vivo source of cardiomyocytes (CMs) for disease modeling, cardiotoxicity screening, drug discovery and cell‐based therapies. However, as shown in previous studies conducted by our lab (Poon, Kong et al. 2011), human embryonic stem cells (hESCs)‐derived CMs display immature〖Ca〗^(2+)–handing properties with smaller transient amplitudes, slower rise and decay kinetics than those of adult CMs. Although the cytosolic 〖Ca〗^(2+) signaling of hESC‐CMs has only recently been understood, there is no investigation on the nuclear 〖Ca〗^

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26

Di, Guglielmo Claudia. "Biotechnological approaches to cardiac differentiation of human induced pluripotent stem cells." Doctoral thesis, Universitat de Barcelona, 2016. http://hdl.handle.net/10803/385921.

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The heart can be considered the most important organ of our body, as it supplies nutrients to all the cells. When affected from injuries or diseases, the heart function is hampered, as the damaged area is substituted by a fibrotic scar instead of functional tissue. Understanding the mechanisms leading to heart failure and finding a cure for cardiac diseases represents a major challenge of modern medicine, since they are the leading cause of death and disability in Western world. Being the heart a vital organ it is difficult to have access to its cells, especially in humans. In order to model i

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27

Wongtrakoongate, Patompon. "Functional study of DNMT3B in human pluripotent and nullipotent stem cells." Thesis, University of Sheffield, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.555240.

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Human embryonic stem (ES) cells and embryonal carcinoma (EC) cells are the stem cells derived from human blastocysts and teratocarcinoma germ cell tumors, respectively. The majority of human EC cell lines have been shown to be nullipotent stem cells, which are not competent to be induced to differentiation both in vitro and in vivo compared with their pluripotent counterparts. However, by knocking down OCT4 it has been shown to induce differentiation of nullipotent EC cells. In this thesis, gene expression analysis has shown that these two stem cell types are different in term of inner cell ma

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28

Jayasundar, Smruthi. "Early molecular events conferring haematopoietic potential to human pluripotent stem cells." Thesis, University of Cambridge, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.607902.

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29

Yamashiro, Chika. "Generation of human oogonia from induced pluripotent stem cells in vitro." Kyoto University, 2019. http://hdl.handle.net/2433/242826.

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30

Abraham, Sheena. "Extracellular matrix based substrates for propagation of human pluripotent stem cells." VCU Scholars Compass, 2010. http://scholarscompass.vcu.edu/etd/92.

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In human pluripotent stem cell (hPSC) research and applications, the need for a culture system devoid of non-human components is crucial. Such a system should exhibit characteristics observed in conventional culture systems that have used mouse embryonic fibroblast feeders for hPSC self renewal without the requirement of excessive supplementation with growth factors. To achieve this, we focused on the identification and characterization of extracellular matrix (ECM) substrates for hPSC propagation. ECM substrates derived from mouse and human fibroblasts were assessed for their ability to suppo

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31

Ohta, Ryo. "Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells." Kyoto University, 2017. http://hdl.handle.net/2433/225982.

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32

Rohani, Leili, Claire Fabian, Heidrun Holland, et al. "Generation of human induced pluripotent stem cells using non-synthetic mRNA." Universitätsbibliothek Leipzig, 2016. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-205889.

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Here we describe some of the crucial steps to generate induced pluripotent stemcells (iPSCs) usingmRNA transfection. Our approach uses a V. virus-derived capping enzyme instead of a cap-analog, ensuring 100% proper cap orientation for in vitro transcribedmRNA. V. virus\' 2′-O-Methyltransferase enzymecreates a cap1 structure found in higher eukaryotes and has higher translation efficiency compared to other methods. Use of the polymeric transfection reagent polyethylenimine proved superior to other transfection methods. The mRNA created via this method did not trigger an intracellular immune res

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33

Thwaites, J. W. "Methods affecting neuronal differentiation of human adult and pluripotent stem cells." Thesis, University College London (University of London), 2015. http://discovery.ucl.ac.uk/1467088/.

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Stem cells have significant potential to treat many age-related degenerative disorders that affect increasing numbers of people globally. This thesis investigated the capacity for omnicytes and human pluripotent stem cells (hPSC) to undergo directed differentiation towards neuronal cell types for the treatment of ischemic stroke and Parkinson’s disease respectively. Omnicytes express a range of markers related to pluripotency and plasticity; however they are a challenging cell source to use in the development of cell therapies. Variability in omnicyte quality was associated with patient source

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34

Chen, Xike. "Integration Capacity of Human Induced Pluripotent Stem Cell-Derived Cartilage." Kyoto University, 2019. http://hdl.handle.net/2433/242390.

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35

Lau, Kei-ling Kelly, and 劉己綾. "Human pluripotent stem cells as a source of dendritic cells to induce immune tolerance." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2013. http://hdl.handle.net/10722/197516.

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Dendritic Cells (DCs) are professional antigen presenting cells that play a crucial role in the induction of immune tolerance. Although DCs have been a potential target for immunotherapy, the amount of DCs in blood source is limited and ex vivo expansion has been inefficient. Human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) provide a great source in cell-based therapy because of their self-renewal ability and pluripotency. My project focuses on generating tolerogenic DCs (tDCs) from human pluripotent stem cells (i.e. hESCs and iPSCs) and their characterization.

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36

Hrvatin, Sinisa. "Exploring the Use of Human Pluripotent Stem Cells to Create Functional Pancreatic \(\beta\) Cells." Thesis, Harvard University, 2012. http://dissertations.umi.com/gsas.harvard:10728.

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Directed differentiation of human pluripotent stem cells (hPSCs) has the potential to produce human cell types that can be used for disease modeling and cell transplantation. Two key challenges in the differentiation from hPSCs to \(\beta\) cells are the specification from pancreatic progenitors to insulin-expressing \((INS^+ )\) cells and the maturation of \(INS^+\) cells into glucose responsive β cells. To address the first, two high-content chemical screens identified PKC inhibitors as inducers of \(INS^+\) cells from pancreatic progenitors. PKC inhibition generated up to tenfold more \(INS

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37

Awaya, Tomonari. "Selective Development of Myogenic Mesenchymal Cells from Human Embryonic and Induced Pluripotent Stem Cells." Kyoto University, 2013. http://hdl.handle.net/2433/180602.

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38

Gotoh, Shimpei. "Generation of Alveolar Epithelial Spheroids via Isolated Progenitor Cells from Human Pluripotent Stem Cells." Kyoto University, 2015. http://hdl.handle.net/2433/195967.

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Final publication is available at http://dx.doi.org/10.1016/j.stemcr.2014.07.005. Shimpei Gotoh, Isao Ito, Tadao Nagasaki, Yuki Yamamoto, Satoshi Konishi, Yohei Korogi, Hisako Matsumoto, Shigeo Muro, Toyohiro Hirai, Michinori Funato, Shin-Ichi Mae, Taro Toyoda, Aiko Sato-Otsubo, Seishi Ogawa, Kenji Osafune, Michiaki Mishima, Generation of Alveolar Epithelial Spheroids via Isolated Progenitor Cells from Human Pluripotent Stem Cells, Stem Cell Reports, Volume 3, Issue 3, 9 September 2014, Pages 394-403, ISSN 2213-6711.<br>Kyoto University (京都大学)<br>0048<br>新制・課程博士<br>博士(医学)<br>甲第18681号<br>医博第395

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39

Haines, Lauren E. "Mesenchymal Analysis of Human Pluripotent Stem Cell-Derived Gastrointestinal Organoids." University of Cincinnati / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1563873084551926.

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40

Carr, Jonathon M. "Heterogeneity within the stem cell compartment : impact on fate determination of human pluripotent stem cells." Thesis, University of Sheffield, 2018. http://etheses.whiterose.ac.uk/20386/.

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41

Ranna, El Khairi Ranna. "The use of human pluripotent stem cells to model HNF1B-associated diabetes." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/284631.

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Heterozygous mutations in the transcription factor, hepatocyte nuclear factor 1B (HNF1B), result in multisystem disease including diabetes due to beta-cell dysfunction and pancreatic hypoplasia. However, the mechanisms that underlie development of diabetes in HNF1B mutation carriers are still not fully understood due to lack of an appropriate model system. Human induced pluripotent stem cells (hiPSCs), which are capable of self-renewal and can differentiate into any cell type, provide an advantageous alternative to model human developmental diseases. The aim of this project was to develop a hi

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42

Richardson, S. E. "Modeling TEL-AML1 childhood acute lymphoblastic leukaemia using human pluripotent stem cells." Thesis, University College London (University of London), 2016. http://discovery.ucl.ac.uk/1496130/.

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Childhood acute lymphoblastic leukaemia (cALL) is distinct from that in adults with higher incidence, better prognosis and a distinct mutational spectrum. One hypothesis for this difference is that cALL arises in transient cells unique to early human development. I explored this in ETV6-RUNX1 cALL where evidence from twins and neonatal heel prick testing has shown that this mutation arises in utero and is an initiating event. I hypothesised that human pluripotent stem cells (hPSCs) could model the developmental features of in utero B cell development. In vitro B cell differentiation of hPSCs p

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Madsen, Ralitsa Radostinova. "Investigation of genetic PIK3CA activation in genome-edited human pluripotent stem cells." Thesis, University of Cambridge, 2019. https://www.repository.cam.ac.uk/handle/1810/289436.

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Mosaic, activating mutations in PIK3CA, the gene encoding the catalytic p110α subunit of class IA phosphatidylinositol 3-kinase (PI3K), are the cause of rare, developmental growth disorders collectively known as PIK3CA-Related Overgrowth Spectrum (PROS). Given the pressing need for targeted therapy and evidence for tissue- and cell lineage-specific distribution of PIK3CA mutations in PROS, developmental models of this disease will be a key asset for preclinical drug testing and for a better understanding of PIK3CA activation in development. This PhD project addressed the lack of human, develop

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44

Owaidah, Amani Yousef. "Factors that control the chondrogenic differentiation of human induced pluripotent stem cells." Thesis, University of Bristol, 2014. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.652038.

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Articular cartilage is a white connective tissue covering the ends of long bones to facilitate movement and articulation. Due to the avascular nature of the tissue, it has a limited capacity to repair once damaged. Human induced pluripotent stem cells are suggested as an ethical and ultimate source for cell based-therapies. Tissue engineering using these cells might present as a promising treatment for cartilage defects. Previous tissue engineering attempts using multipotent stem cells such as adult msenchymal stem cells or pluripotent stem cells such as embryonic stem cells were qualitative,

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45

Campbell, Ian. "Optimization of Methods for Generating Customized Gene-Edited Human Pluripotent Stem Cells." University of Cincinnati / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1504802720510926.

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46

Bai, Qiang. "Human pluripotent stem cells in In vitro conditions : differentiation and genomic instability." Thesis, Montpellier 1, 2013. http://www.theses.fr/2013MON1T007/document.

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Les cellules souches pluripotentes humaines (hPSC) sont des cellules capables à la fois d'autorenouvellement et de se différencier en tous les types cellulaires. Elles peuvent être issues de l'embryon (pour cellules souches embryonnaires humaines, hESC) ou être obtenues par reprogrammation d'une cellule différenciée (pour cellules souches pluripotentes induites humaines, hiPSC). Les hPSC sont au centre d'enjeux scientifiques, médicaux et économiques majeurs, en particulier dans le cadre des maladies génétiques et orphelines. En effet, elles ouvrent la porte à de nouvelles stratégies de modélis

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47

Kajiwara, Masatoshi. "Donor-dependent variations in hepatic differentiation from human-induced pluripotent stem cells." Kyoto University, 2013. http://hdl.handle.net/2433/170075.

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48

Hackett, Lucy Ann. "The investigation of models to identify and quantitate human epidermal stem cells." Thesis, Queen Mary, University of London, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.322406.

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49

Kaushik, Suresh Kumar. "Genetic modification of human embryonic stem cells for lineage selection, derivation and analyses of human 3rd pharyngeal pouch epithelium like cells and its derivatives." Thesis, University of Edinburgh, 2017. http://hdl.handle.net/1842/28724.

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Human pluripotent stem cells (hPSCs) such as, human embryonic stem cells (hES) and human induced pluripotent stem cells (hiPS) are a valuable resource to generate bespoke cell types for a number of therapeutic applications involving cell therapy, drug screening and disease modelling. The overarching goal of this project was to generate a set of transgenic tools by gene targeting and genetic modification of hESCs for applications in stem cell biology such as the in vitro isolation, analyses and derivation of lineage specific cell types. The transgenic tools generated in this study were designed

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Ould-Brahim, Fares. "Preconditioning of Human Neural Stem Cells with Metformin to Promote Post-Stroke Recovery." Thesis, Université d'Ottawa / University of Ottawa, 2018. http://hdl.handle.net/10393/37094.

Full text

Abstract:

The generation of human induced pluripotent stem cells (hiPSCs) from human fibroblasts has revolutionized cell therapy by providing a source of autologous cells for transplantation. Several studies have demonstrated that transplantation of hiPSC-derived neural stem cells (hiPSC-NSCs) increases regeneration and recovery following stroke, supporting their therapeutic potential. However, major concerns for translating hiPSC transplantation therapy to the clinic are efficacy and safety. Therefore, there is demand to develop an optimal strategy to enhance the engraftment and regenerative capacity o

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