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Amorín, Milagros, Andrea Carlina, and Ana Prötzel. "Mucopolisacaridosis de tipo I Hurler: Informe de un caso." Sociedad Argentina de Pediatría (SAP), 2014. http://hdl.handle.net/10757/313689.
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Mucopolysaccharidosis I (MPS I) is a rare, recessively inherited, lysosomal storage disorder caused by deficiency on the enzyme α-L-iduronidase. This defect results in accumulation of heparan and dermatan sulfate in different tissues and organs due to a deficiency in the catabolism of glycosaminoglycans. The overall incidence of MPS I is 0.99-1.99/100.000 live births. There are three clinical presentations: Hurler (severe), Hurler Scheie (mild) and Scheie (mild). We report the case of a 10-years-old male patient diagnosed with Hurler syndrome, the severe presentation, 5 years ago by enzyme α-L-iduronidase activity measurement in leukocytes; with a history of recurrent respiratory infections, umbilical hernia, corneal opacity, coarse facial features, macroglossia, hearing loss, stiffness of joints, cardiac compromise, claw hands, mental retardation and stunted growth. After enzyme replacement therapy the patient has shown improvement of visceral symptoms, but the neurological damage continuous in progress. Key words: mucopolysaccharidosis, Hurler syndrome, MPS 1, Hurler, genetic disorder, enzymatic replacement therapy, ERT.<br>Revisión por pares
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Nelson, John. "The mucopolysaccharidoses in Northern Ireland : a clinical, genetic and biochemical study." Thesis, Queen's University Belfast, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.357486.
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Hurler, Bernhard Alexander [Verfasser], and M. [Akademischer Betreuer] Zitterbart. "Dienstorientierte Sensornetze: Programmierabstraktionen und Dienstkomposition / Bernhard Alexander Hurler. Betreuer: M. Zitterbart." Karlsruhe : KIT-Bibliothek, 2012. http://d-nb.info/1033351539/34.
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MONTAGNA, ANNA. "Induced pluripotent stem cells (IPSCS) for modelling mucopolysaccharidosis type I (Hurler syndrome)." Doctoral thesis, Università degli Studi di Milano-Bicocca, 2016. http://hdl.handle.net/10281/113869.
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Mucopolysaccharidosis type I (MPS-IH or Hurler syndrome) is a rare lysosomal storage disease caused by mutations in the IDUA gene, resulting in the deficiency of alpha-L-iduronidase (IDUA) enzyme activity with a consequent intracellular accumulation of glycosaminoglycans (GAGs). Among a broad spectrum of clinical manifestations, MPS-IH is characterized by a range of skeletal abnormalities known as dysostosis multiplex. To date, the skeletal pathogenesis of the MPSs has been assumed to be directly related to the progressive storage of GAGs. It is now clear that more complex cellular and molecular mechanisms underlie the patient clinical symptoms. Therefore, an appropriate humanized in vitro model is highly recommended to highlight these mechanisms. Compared to mesenchymal stromal cells (MSCs), induced pluripotent stem cells (iPSCs) represent a useful tool to achieve this purpose, due to their high proliferation capability in culture and, mostly, to their ability to mimic development. Thus, they demonstrate great potential for investigating the osteogenic differentiation process.
In this study, we generated MPS-IH patient-specific iPS cells (MPS-IH iPSCs) which maintained the genetic mutation in the IDUA gene and, as a consequence, reduced IDUA enzyme activity and GAGs intracellular accumulation.
In order to assess if the osteogenic differentiation phenotype is already compromised in MPS-IH iPSCs cell, we focused on their bone differentiation capability. Thus, we developed an osteogenic differentiation protocol through the generation of mesenchymal stromal cells from iPSC (hereafter named MSCs-like cells).
We designed a robust, multistep differentiation method to isolate MSCs-like cells, both from wild-type iPSCs (WT-iPSCs) and MPS-IH iPSCs. The process included: embryoid body (EB) formation, cell outgrowth from EBs, monolayer culture of sprouted cells from EBs, and a serial of passages in culture until they reached a fibroblast-like morphology and the full expression of mesenchymal surface markers.
Firstly, we characterized WT and patient derived-MSCs-like cells in terms of morphology, phenotype, proliferation kinetics and differentiation capacity in mesodermal tissues. WT and patient derived-MSCs-like cells showed the capacity to differentiate in adipocytes, as confirmed by Oil Red O staining. Moreover, MSCs-like cells derived-chondrogenic pellets exhibited a spherical, compact morphology. Histological analysis revealed an initial chondrogenic differentiation, as confirmed by q-RT-PCR for key early chondrogenic markers, such as SOX9 and COLII.
Subsequently, we developed an osteogenic differentiation protocol for the obtained MSC-like cells. In order to verify if the differentiation process was accomplished, we performed Alizarin Red staining and quantified the hydroxyapatite production by colorimetric detection at 405 nm both on WT and MPS-IH iPSCs-derived osteoblasts. At the same time, we examined the expression for key osteogenic markers, such as OPN, RUNX2 OTC, OTN, ALP and COL 1A2, through q-RT-PCR. Recently, our group isolated MSCs from bone marrow (BM-MSCs) of both healthy donors and MPS-IH patients, studying a possible involvement of MSCs in the skeletal abnormalities affecting Hurler patients. We previously observed the ability of WT, MPS-IH BM-MSCs and MSCs-derived osteoblasts to stimulate osteoclastogenesis in vitro by measuring the molecular levels of receptor activator of nuclear factor-Kb ligand (RANKL) and osteoprotegerin (OPG), two key partners of the system directly regulating osteoclast differentiation. MPS-IH MSCs and osteoblasts derived from MPS-IH MSCs, expressed a higher level of RANKL compared to HD-MSCs and osteoblasts. OPG level, instead, was similar.
In the present study, the osteogenic differentiation protocol developed allowed us to assess if this altered phenotype is already evident in both MSCs-like cells MSCs-like derived osteoblasts, by evaluating the OPG and RANKL expression levels.
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Hurler, Wolfgang [Verfasser]. "Fusions- und seltene Lageanomalien der Niere: Symptome, Morbidität und klinische Relevanz / Wolfgang Hurler." Ulm : Universität Ulm. Medizinische Fakultät, 2012. http://d-nb.info/1022896393/34.
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Pitt, Cheryl Anne. "Psychosocial outcomes of bone marrow transplant for individuals affected by MPS I Hurler Disease." Thesis, Bucks New University, 2009. http://bucks.collections.crest.ac.uk/9769/.
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RATIONALE: A theoretical model was used to examine the impact of risk and resistance factors on the psychosocial adjustment of children and young people affected by Mucopolysaccharidosis Type I Hurler Disease post-bone marrow transplant. METHOD: A sequential exploratory mixed methods design was employed and the study carried out in two phases. In the initial phase qualitative methods were employed in an in-depth study of a small sample of parents of individuals affected by this condition (n=10). They were administered semi-structured interviews, which were analysed using Interpretative Phenomenological Analysis. The themes highlighted were used to inform the design of the second phase, which employed quantitative methods. In this phase forty-four families affected by MPS IH post-BMT participated (44 mothers, 36 fathers, 44 patient participants). This comprised almost the entire population of this patient group in the UK, whose ages ranged from 16 months to 25 years. A face-to-face survey method was used with the mothers, telephone-survey with the fathers and psychometric testing of the patient participants. The measures included risk factors (e.g. physical, cognitive, and adaptive functioning), resistance factors (intrapersonal, stress processing, and socialecological), and adjustment for both parent and patient participants. RESULTS: Data from the qualitative phase revealed a number of themes, highlighting numerous issues pertinent to parents of children affected by this condition. These included perceptions of child vulnerability, uncertainty, perceptions of stress and sources of support, and feeling that others do not understand their situation. Data from the second phase illustrate how stress processing and social-ecological factors make significant contributions to parent adjustment. They also illustrate how intrapersonal, socialecological, and maternal stress processing factors contribute significantly to patient adjustment. A theoretical path of the determinants of patient psychosocial outcomes was created to illustrate these relationships. CONCLUSION: The findings are indicative of how resistance factors moderate the effects that disease- and disability-related risk factors have on parent and patient adjustment as predicted by the model. The dynamic interplay between disease-related risk factors and intrapersonal, socialecological, and stress processing factors are discussed in relation to parent and patient adjustment outcomes, as are the implications these relationships pose for patient and family support.
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GATTO, FRANCESCA. "Exploring hurler syndrome through the study of disease-specific multipotent and pluripotent stem cells." Doctoral thesis, Università degli Studi di Milano-Bicocca, 2012. http://hdl.handle.net/10281/29890.
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Mucopolysaccharidosis type I (or Hurler syndrome) is a rare genetic disorder, caused by mutations in the idua gene, resulting in the deficiency of α-L-iduronidase enzyme activity and intra-cellular accumulation of glycosaminoglycans. The aim of the present project was focused on the isolation and characterization of two different stem cell populations, multipotent and pluripotent, derived from patients affected by Hurler syndrome. We aim to use Mesenchymal Stem Cells (MSCs) and induced Pluripotent Stem Cells (iPSCs) as a tool to explore still unknown disease mechanisms involved in the genetic metabolic disorder of our interest.
Our recently published study focused on the characterization of MSCs isolated from bone marrow of Hurler patients. The MSCs were characterized for their expansion rate, phenotype, telomerase activity, IDUA activity and differentiation capacity towards adipocytes, osteoblasts, chondrocytes and smooth muscle cells in vitro. Interestingly, affected MSCs displayed increased capacity to support osteoclastogenesis according to the upregulation of the RANKL/RANK/OPG molecular pathway in Hurler MSCs.
The second study describes the isolation of iPSCs from fibroblasts of Hurler patients. The generated cell lines were fully characterized for their pluripotency markers, gene expression profile, viral copy number integration and differentiation potential both in vitro and in vivo. As a proof of principle, we are attempting to gene correct patient-derived iPSCs with an alternative and safer method than viral vectors, using a Zinc Finger Nucleases-mediated approach for gene targeting of pluripotent cells.
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Federhen, Andressa. "Mucopolissacaridose tipo I: avaliação de um novo instrumento para classificação fenotípica." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2012. http://hdl.handle.net/10183/52961.
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Introdução: A mucopolissacaridose tipo I é comumente classificada em três síndromes clínicas (Hurler, Hurler-Scheie e Scheie), de acordo com a gravidade do fenótipo. Devido à alta heterogeneidade da doença e à sobreposição de sintomas em pacientes, alguns autores consideram esta forma de classificação ultrapassada e defendem que a doença apresenta um espectro fenotípico mais amplo. Para tanto, seria muito útil o desenvolvimento de novas ferramentas que possam contribuir para uma melhor classificação dos pacientes. Objetivo: Avaliar um novo instrumento para a classificação fenotípica da MPS I e verificar a correlação dos resultados obtidos com o mesmo com as características bioquímicas e moleculares dos pacientes avaliados. Materiais e Método: Um instrumento elaborado por um grupo de especialistas foi por nós adaptado para avaliar a gravidade do fenótipo ao diagnóstico de 43 pacientes brasileiros com MPS I. Uma nota de zero a 14 foi obtida com a aplicação deste instrumento pela avaliação da presença ou ausência dos seguintes sinais e sintomas ao diagnóstico: atraso no desenvolvimento neuropsicomotor e/ou declínio cognitivo, rigidez articular/artropatia/contraturas articulares, cifose, disostose multiplex, macrocefalia e bossa frontal. Os pacientes incluídos foram também avaliados em relação aos mesmos parâmetros do instrumento e quanto ao seu fenótipo por três geneticistas com reconhecida experiência com essa doença. Dados bioquímicos e moleculares também foram utilizados para comparação com os resultados da aplicação do instrumento. Resultados: Os pontos de corte com melhor balanço de sensibilidade e especificidade encontrados a partir da aplicação do instrumento e a partir da avaliação dos geneticistas foram, respectivamente 7 e 9. Os parâmetros do instrumento para os quais foi observada diferença estatisticamente significativa foram atraso do desenvolvimento neuropsicomotor/declínio cognitivo (entre o fenótipo Hurler e os fenótipos Hurler-Scheie e Scheie) e disostose múltipla (entre os fenótipos Hurler e Scheie). Não foi observada correlação entre a nota obtida no instrumento e os valores de GAGs urinários, nos diferentes fenótipos. A maior parte dos pacientes com mutações sem sentido foi classificada como grave, tanto a partir do instrumento quanto pela avaliação dos geneticistas. Conclusões: Os pontos de corte encontrados podem ser úteis para a classificação dos pacientes em dois grupos distintos – grave e atenuado. O atraso no dsenvolvimento neuropsicomotor/declínio cognitivo e a disostose múltipla são achados importantes para predizer a gravidade da doença. Mutações sem sentido parecem determinar o fenótipo mais grave da síndrome. Não foi possível distinguir os diferentes fenótipos a partir dos valores de GAGs na urina. A ferramenta desenvolvida parece ser útil para auxiliar na classificação da gravidade da MPS I, mas é recomendável sua aplicação em um número maior de pacientes para melhor dimensionar sua potencial aplicação.<br>Introduction: Mucopolysaccharidosis type I is usually classified into three clinical syndromes (Hurler, Hurler-Scheie and Scheie) according to the severity of the phenotypic expression. Some authors believe this classification is not accurate because of disease variation and overlapping findings in some patients, and suggest that its phenotypic spectrum is wider. With this view, it would be useful the development of new tools which could contribute to a better classification of patients. Objective: To evaluate a new tool the phenotypic classification of MPS I and investigate whether the results obtained are correlated with biochemical and molecular characteristics of the patients. Material and methods: A tool developed by a group of specialists was adapted by us for the evaluation of the phenotypic severity at diagnosis in 43 Brazilian patients with MPS I. A score of zero to 14 was obtained using this tool, which evaluated presence or absence of the following signs and symptoms: delay in neurological and psychomotor development and cognitive decline; joint stiffness, arthropathy and joint contractures; kyphosis; dysostosis multiplex; macrocephaly; and frontal bossing. The same patients had the same parameters evaluated by three MDs with expertise in MPS who also provided their impression about the phenotype. Biochemical and molecular findings were also compared with the results obtained in the proposed tool. Results: The cut-off points with better balance of sensitivity and specificity found with the use of the tool and according to the experts' evaluations were 7 and 9, respectively. The tool parameters with statistically significant differences were neurological and psychomotor development delay and cognitive decline (when the Hurler phenotype was compared with the Hurler-Scheie and Scheie phenotypes), and dysostosis multiplex (for the comparison between the Hurler and Scheie phenotypes). There was no correlation between the scores obtained when using the tool and the urinary GAG values across the different phenotypes. Most patients with nonsense mutations were classified as severe according to the tool scores and to the experts’ evaluations. Conclusions: The cut-off points found in this study may be useful for the classification of patients into two distinct groups - severe and attenuated. Neurological and psychomotor developmental delay and cognitive decline, as well as dysostosis multiplex, are important findings to predict disease severity. Nonsense mutations seem to determine the most severe syndrome phenotype. The urinary GAG values do not allow differentiating the different phenotypes. The tool developed seems to be useful to help in the classification of the severity of MPS I, but it would be advisable its application in a larger number of patients to better evaluate its potential application.
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PENNA, SARA. "Development of novel cell based therapeutic approaches to correct primary and secondary bone defects." Doctoral thesis, Università degli Studi di Milano-Bicocca, 2021. http://hdl.handle.net/10281/304794.
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Le malattie scheletriche pediatriche compromettono fortemente la durata della vita. Disturbi monogenici rari e gravi come l'osteopetrosi autosomica recessiva (ARO) e la mucopolisaccaridosi di tipo 1 Hurler (MPSIH) sono causati rispettivamente da difetti ossei primari e secondari. In particolare, i pazienti con ARO soffrono di elevata densità e fragilità ossea, difetti neurologici e fibrosi del midollo osseo che portano ad un aumento del numero di cellule CD34+ circolanti. La forma più frequente di ARO è dovuta alle mutazioni del gene TCIRG1, che codifica per una pompa protonica necessaria per l'attività di riassorbimento osseo degli osteoclasti. La sindrome MPSIH è uno dei disturbi da accumulo lisosomiale più frequenti, causato da mutazioni del gene IDUA, che codifica per l'enzima alfa-L-iduronidasi. L'enzima IDUA difettoso causa un ingolfamento lisosomiale dovuto al ridotto turnover dei glicosaminoglicani (GAG), portando a gravi disfunzioni degli organi e ad anomalie scheletriche. La patogenesi dei difetti ossei in MPSIH è ancora ampiamente dibattuta. Il trapianto allogenico di cellule staminali ematopoietiche (HSCT) è l'approccio standard per i pazienti ARO e MPSIH, ma l'alta incidenza di esiti avversi e la scarsa disponibilità di donatori compatibili, aprono la strada allo sviluppo di strategie di terapia genica (GT) per curare queste malattie.
Nella presente tesi abbiamo sviluppato una nuova strategia di GT basata su vettori lentivirali clinicamente ottimizzati esprimenti il gene TCIRG1. Abbiamo testato il nostro protocollo di GT sul modello di topo oc/oc, molto simile alla malattia umana, con un'aspettativa di vita di 2-3 settimane. I topi GT hanno raggiunto fino a quattro mesi di età, mostrando un miglioramento del fenotipo osseo e dello stato clinico generale.
In parallelo, le cellule CD34+ isolate dal sangue di pazienti ARO sono state caratterizzate fenotipicamente in termini di composizione di cellule staminali e progenitori ematopoietici e analizzate dal punto di vista del trascrittoma. Inoltre, le cellule CD34+ circolanti di pazienti ARO sono state trasdotte ed espanse, applicando un protocollo che consente il mantenimento della staminalità. Abbiamo inoltre eseguito test in vitro per valutare la capacità di riassorbimento degli osteoclasti derivati dai pazienti ARO e abbiamo valutato il potenziale di ripopolamento multi-lineage a lungo termine delle cellule CD34+ espanse mediante trapianto primario e secondario in topi NSG.
Per quanto riguarda MPSIH, la sperimentazione clinica per la GT è in corso presso SR-Tiget (NCT03488394), e ha mostrato miglioramenti nei difetti scheletrici e nell'attività dell’enziama IDUA dei pazienti MPSIH. Abbiamo studiato la funzionalità degli osteoclasti e il loro ruolo nel fornire l'enzima IDUA nel microambiente osseo, correggendo le cellule stromali mesenchimali e la loro progenie dopo la GT. A tal fine, abbiamo differenziato gli osteoclasti dal sangue o dal midollo osseo di pazienti con MPSIH pre e post-GT, osservando che gli osteoclasti trasdotti producono livelli sovrafisiologici di IDUA, creando così un modello per lo studio del cross talk osteoblasto-osteoclasto. I nostri risultati suggeriscono che la GT rappresenta un trattamento alternativo possibile ed efficace per la cura dell’osteopetrosi TCIRG1-dipendente e della sindrome di Hurler.<br>Pediatric skeletal diseases strongly impair the lifespan of young children. Rare and severe monogenic disorders like Autosomal Recessive osteopetrosis (ARO) and Mucopolysaccharidosis type 1 Hurler (MPSIH) are caused by primary and secondary bone defects, respectively. In particular, ARO patients suffer from high bone density and fragility, neurological defects and bone marrow fibrosis leading to increased number of circulating CD34+ cells. The most frequent form of ARO is due to mutations in TCIRG1 gene, that encodes for a proton pump necessary for bone resorptive activity of osteoclasts. MPSIH syndrome is one of the most frequent lysosomal storage disorders, caused by mutations of IDUA gene, that encodes for the alpha-L-iduronidase enzyme. Defective IDUA enzyme causes lysosomal engulfment due to impaired turnover of glycosaminoglycans (GAGs), leading to severe organ dysfunctions and skeletal abnormalities. The pathogenesis of bone defects in MPSIH is still largely debated. Allogeneic haematopoietic stem cells transplantation (HSCT) is the standard approach for ARO and MPSIH patients, but the high incidence of adverse outcomes and the low availability of compatible donors, pave the way for the development of gene therapy (GT) strategies to cure these diseases.
In the present thesis we developed a novel GT strategy based on clinically-optimized lentiviral vectors, driving TCIRG1 expression. We tested our GT protocol on the oc/oc mouse model, closely resembling the human disease, with a life expectancy of 2-3 weeks. GT mice reached up to four months of age, showing an amelioration of the bone phenotype and an improved clinical status.
In parallel, CD34+ cells isolated from the blood of ARO patients were phenotypically characterized in terms of hematopoietic stem and progenitor cells composition and analysed for transcriptome profile. Moreover, ARO circulating CD34+ were transduced and expanded, applying a protocol that allows stemness maintenance. We performed in vitro assays to evaluate resorption capacity of patient-derived osteoclasts and we evaluated the long-term multilineage repopulating potential of expanded CD34+ cells by primary and secondary transplant into NSG mice.
With regard to MPSIH, GT clinical trial is ongoing at SR-Tiget (NCT03488394), ameliorating skeletal defects and rescuing IDUA activity of MPSIH patients. We investigated the functionality of osteoclasts and their role in delivering IDUA enzyme in the bone microenvironment, cross-correcting mesenchymal stromal cells and their progeny after GT. To this end, we differentiated osteoclasts from the blood or bone marrow of MPSIH patients pre- and post-GT, observing that transduced osteoclasts produce supraphysiological levels of IDUA thus modulating osteoblast-osteoclast cross talk. Our results suggest that GT represents a feasible alternative treatment for TCIRG1-dependent ARO and Hurler syndrome.
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Dusing, Stacey Chapman Thorpe Deborah E. "Gross motor and gait abilities of children with Hurler syndrome, pre and post umbilical cord blood transplant." Chapel Hill, N.C. : University of North Carolina at Chapel Hill, 2006. http://dc.lib.unc.edu/u?/etd,266.
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Thesis (Ph. D.)--University of North Carolina at Chapel Hill, 2006.<br>Title from electronic title page (viewed Oct. 10, 2007). "... in partial fulfillment of the requirements for the degree Doctor of Philosophy in the School of Medicine (Curriculum in Human Movement Science)." Discipline: Human Movement Science; Department/School: Medicine.
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Cross, Elaine. "Behavioural phenotypes in the mucopolysaccharide disorders." Thesis, University of Manchester, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.566569.
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This thesis investigated behaviour and behavioural phenotypes in the Mucopolysaccharide (MPS) disorders. The MPS disorders are a group of rare lysosomal storage disorders which are characterised by a period of normal development followed by gradual cognitive and/or physical decline.Paper 1 describes a systematic review of the extant literature on cognitive, motor, social, linguistic and behavioural presentation in all of the MPS disorders. 25 papers were reviewed and the methodology they employed was assessed. Sleep disturbance was found to be part of the behavioural phenotype of MPS III. In MPS I and II fearfulness and sleep problems occurred in most cases. In MPS II participants with the mild form were found to have relatively normal development and few or no behavioural problems, while those with the severe form had behavioural problems, delayed speech, delayed development and limited motor function. High rates of challenging behaviour, most commonly associated with aggression, hyperactivity, orality, unusual affect and temper tantrums were consistently observed in children with MPS III.Paper 2 describes an empirical study investigating the behavioural phenotype of MPS III, Sanfilippo syndrome. Parents of 20 children with MPS III, 5 adults with MPS III and 25 children with Intellectual Disability (ID) completed questionnaires relating to their son/daughter’s behaviour and adaptive skills. The frequency of challenging behaviours displayed by children aged 2-9 years with MPS III and ID were high but not significantly different. Behaviours associated with hyperactivity, orality, body movements and inattention were seen significantly more frequently in 2-9 year olds with MPS III than ID. The frequency of challenging behaviours displayed by children with MPS III and their adaptive skills was found to decrease with age. Children age 10-15 years with MPS III displayed significantly fewer problem behaviours than children of the same age with ID. It is recommended that parents with a child with MPS III aged 2-9 years are offered clinical services to support them with managing challenging behaviour while those with a child of 10 years or over are offered support with managing health concerns and end of life care.The third Paper, provides an evaluation of the strengths and limitations of the literature review and the empirical study. The findings and clinical implications from both studies are discussed. The process of conducting research into rare, life limiting, genetic syndromes is reflected upon and recommendations for replication and further research are made.
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Nabee, Mahomed Ridhwaan Goolam. "Demographic profile, clinical data and radiographic analysis of patients for third molar surgery under general anaesthesia at the Faculty of Dentistry at the University of the Western Cape." University of the Western Cape, 2018. http://hdl.handle.net/11394/6504.
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Magister Scientiae Dentium - MSc(Dent)<br>Aim
To analyze the demographic profile, clinical data and radiographs of patients who had third molar
surgery under general anaesthesia at the Faculty of Dentistry at the University of the Western
Cape over a 10 year period.
Introduction
Minor oral surgical procedures are carried out by Maxillofacial and Oral Surgeons daily. The
surgical removal of third molars is a large part of Minor Oral Surgery which is common
throughout the world. The general impression of third molar surgery performed by experienced
professionals is the ease of the operation, however no-matter how experienced one may be, a
simple procedure should never be underestimated (Carvalho and Do Egito Vasconselos, 2011).
New surgical techniques, as well as extensive training, skill and experience have led to the
evolution of oral surgery and allowed this procedure to be carried out in a less traumatic manner.
Certain factors precipitate third molar surgery to be performed in theatre as opposed to the dental
clinic setting. These factors will be discussed in this research report.
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Almeida, Andresa Cardoso Grandini. "Estabelecimento de um novo método de diagnóstico molecular para mucopolissacaridose tipo I." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2010. http://hdl.handle.net/10183/28361.
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A mucopolissacaridose tipo I (MPS I) é uma doença genética autossômica recessiva, causada pela deficiência da enzima lisossomal α-L-iduronidase (IDUA), envolvida na degradação de dermatan e heparan sulfato. O acúmulo desses substratos nas células provoca inúmeras e variadas manifestações clínicas, acarretando diferentes formas da doença, as quais estão divididas de acordo com a sua gravidade: forma atenuada (Síndrome de Scheie – MPS I S), forma intermediária (Síndrome de Hurler-Scheie – MPS I SH) e forma grave (Síndrome de Hurler – MPS I H). O diagnóstico molecular precoce e preciso pode ser feito pela análise de DNA. Entretanto, como essa abordagem despende bastante tempo e recursos, procurou-se estabelecer uma estratégia mais eficiente de diagnóstico molecular. Inicialmente foi feita a comparação entre a metodologia tradicional e a análise por RNA através de levantamentos comparativos de tempo e custos de ambas as metodologias. A seguir, foi realizada a padronização e a validação dessa técnica. A padronização foi realizada em plasmídeo contendo cDNA de IDUA e a sua validação foi feita utilizando-se sangue e/ou fibroblastos de pacientes com diagnóstico molecular já estabelecido pela metodologia tradicional. Finalmente, foi sugerida uma nova abordagem otimizada de análise de DNA através da análise das freqüências de mutações descritas na literatura. O estudo do RNA pareceu, num primeiro momento, bastante vantajoso, sendo realizado em menos tempo e com custo reduzido para a genotipagem completa do gene. Para pesquisa das mutações comuns, a análise por RNA teve um custo mais alto, porém uma redução de 60% no tempo de processamento. Passou-se para a padronização da técnica, que foi realizada com sucesso. Entretanto, na etapa de validação observou-se que em pacientes com mutações sem sentido ou que alteram o sítio de splice, não são corretamente diagnosticados através dessa metodologia. Apenas as mutações com sentido trocado aparecem claramente, porém ainda assim pacientes heterozigotos compostos para essas mutações e uma mutação sem sentido são incorretamente diagnosticados como homozigotos, devido à falha de amplificação do alelo com mutação sem sentido. Como a abordagem proposta inicialmente não foi satisfatória, procurou-se uma estratégia otimizada de análise de DNA através do seqüenciamento direto de éxons com maior freqüência de mutações. Essa nova abordagem utilizando DNA ainda necessita ser validada em uma amostra de pacientes para que se possa conhecer o seu real impacto no tempo e custo do diagnóstico molecular de pacientes com MPS I.<br>Mucopolisaccharidosis type I (MPS I) is an autosomal recessive genetic disorder caused by the deficiency of the lysosomal enzyme α-L-iduronidase (IDUA), responsible for the degradation of dermatan and heparan sulfate. The storage of these undegraded or partially degraded substrates causes the various clinical manifestations of the disease that can be classified into three main forms: Scheie Syndrome (MPS I S), the attenuated form; Hurler- Scheie Syndrome (MPS I HS), the intermediate form; and Hurler Syndrome (MPS I H), the severe form. Early and precise molecular diagnosis can be performed by DNA analysis. However, as this methodology is time and resource-consuming, more efficient approaches are needed. Initially, a comparison between the traditional DNA analysis and RNA-based analysis was performed for each step of both techniques. Then, the RNA-based technique was set up using a plasmid with IDUA cDNA and validated on patients with molecular diagnosis already performed by DNA analysis. Finally, an optimized strategy for DNA-based analysis based on the mutation frequencies was proposed. In comparison with DNA-based analysis, RNA studies seem more advantageous, as it is less expensive and faster for whole gene sequencing. For the screening of common mutations it is more expensive but 60% less time-consuming. This technique was successfully set-up. However, at the validation step it was noticed that nonsense and splice site mutations are not correctly diagnosed by this methodology. Missense mutations can be clearly identified even though compound heterozygotes with nonsense mutations are wrongly diagnosed as homozygotes due to amplification failure of the nonsense carrying allele. As this approach was considered unsuitable for the analysis of MPS I patients, a new optimized strategy based on direct sequencing of selected exons was suggested. This approach still needs to be validated in a sample of patients so that its real impact on molecular diagnosis of MPS I can be established.
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GUICHARD, EVELYNE. "Greffes allogeniques de moelle osseuse dans les maladies de hurler, hunter et leucodystrophie metachromatique : a propos de cinq cas personnels avec revue de la litterature." Saint-Etienne, 1991. http://www.theses.fr/1991STET6234.
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Dias, Chloé. "Caractérisation des vésicules extracellulaires et étude de leur rôle dans la neuropathologie de la mucopolysaccharidose de type III." Electronic Thesis or Diss., Université de Toulouse (2023-....), 2024. http://www.theses.fr/2024TLSES084.
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La mucopolysaccharidose de type III (MPS III) ou syndrome de Sanfilippo est une maladie pédiatrique rare, d'origine génétique, dite de surcharge lysosomale (MSL) et causée par le déficit en enzymes impliquées dans la dégradation de l'héparane sulfate. Le diagnostic tardif et l'absence de traitement curatif entrainent le décès de l'enfant avant l'âge adulte. Cependant les derniers essais cliniques de thérapie génique chez des enfants atteints de MPS III de type B ont donné des résultats prometteurs. L'accumulation progressive d'oligosaccharides d'héparane sulfate (HSOs) anormaux et partiellement dégradés dans le milieu extracellulaire, active directement la production de cytokines pro-inflammatoires par les astrocytes et la microglie. La mise en place précoce d'une neuroinflammation chronique conduit progressivement à la mort neuronale, à l'origine de troubles neurodégénératifs sévères et irréversibles. Les mécanismes cellulaires liés à la propagation de la neuroinflammation ne sont pas encore élucidés, et une des hypothèses est qu'elle pourrait être médiée par la sécrétion de vésicules extracellulaires (EVs). Chez l'adulte atteint de troubles neurodégénératifs, des travaux récents ont démontré que les EVs provenant de cellules activées et notamment de la microglie, sont chargées d'un contenu moléculaire pathologique spécifique et suscitent un vif intérêt lié à leur utilisation comme biomarqueurs. L'objectif de mon projet de thèse est d'investiguer la contribution des EVs dans la physiopathologie de la MPS III, par i) l'analyse de la composition en protéines et en ARNs des EVs-MPS III et ii) l'évaluation de la réponse biologique des neurones corticaux primaires naïfs au message transmis par ces EVs-MPS III. Dans ce but, j'ai isolé et étudié les EVs provenant de deux modèles de la maladie, le surnageant d'une lignée microgliale activée avec des HSOs de patients MPS III et les tissus cérébraux du modèle murin MPS IIIB. Par l'analyse de la composition en protéines et micro ARNs des EVs, j'ai pu mettre en évidence que les EVs-MPS III transmettent aux cellules réceptrices du système nerveux central (SNC) un cargo altéré et spécifique impliqué dans la réponse inflammatoire et dans le développement neuronal. Ensuite, grâce à des expériences de transmission des EVs-MPS III sur des neurones sauvages corticaux primaires, j'ai pu démontrer, par microscopie à fluorescence, que la morphologie des compartiments somato-dendritiques est fortement altérée. Ensuite, en utilisant des EVs isolés de cerveaux de souris MPS IIIB âgées de 1 mois, 4 mois et 8 mois, j'ai montré que les EVs induisaient une signature transcriptionnelle des neurones différente en fonction des stades de la maladie. En résumé, ces résultats suggèrent fortement que chez les patients atteints du syndrome de Sanfilippo, les EVs participent activement à la progression de la pathologie et au dysfonctionnement des neurones, certainement par la propagation de l'inflammation et l'altération des mécanismes de neurotransmission. Mon travail de thèse a permis de décrire dans nos modèles, que les EVs-MPS III transportent un contenu spécifique et caractéristique de la pathologie et de son évolution. Ces découvertes offrent la perspective d'utilisation des EVs comme biomarqueurs d'évaluation de l'efficacité thérapeutique pour les patients inclus dans les futurs essais cliniques<br>Mucopolysaccharidosis type III (MPS III) or Sanfilippo syndrome is a rare pediatric inherited disease classified as a lysosomal storage disorder (LSD), caused by the deficiency of enzymes involved in the degradation of heparan sulfate (HS). Late diagnosis and lack of curative treatment lead to the death of the child before adulthood. However, the latest gene therapy clinical trial in children affected by MPS III type B have showed promising results. The progressive accumulation of abnormal and partially degraded heparan sulphate oligosaccharides (HSOs) in the extracellular space, directly activates the production of pro-inflammatory cytokines by astrocytes and microglia. The early onset of neuroinflammation leads progressively to neuronal death, causing severe and irreversible neurodegenerative deficiencies. The cellular mechanisms involved in the propagation of the neuroinflammation remains to be elucidated, and one hypothesis is that it could be mediated through the secretion of extracellular vesicles (EVs). In adults with neurodegenerative disorders, recent works demonstrated that EVs, derived from activated cells and in particular from microglia, are loaded with specific pathological molecular content and are arousing great interest for their use as biomarkers. The objective of my PhD is to investigate the contribution of EVs in the pathophysiology of MPS III, by i) analyzing the composition in proteins and RNAs of MPS III-EVs and ii) studying the biological response of naïve primary cortical neurons to the message transmitted by these MPS III-EVs. For this purpose, I isolated and studied EVs from two models of the disease, the supernatant of a microglia cell line activated by HSOs of MPS III patients and the brain tissues of the MPS IIIB mouse model. By analyzing the proteins and micro RNAs composition of EVs, I was able to demonstrate that MPS III-EVs transmit to the recipient cell of the central nervous system (CNS) a specific and altered cargo involved in inflammatory response and in neuronal development. Then, through transmission experiments of MPS III-EVs to wild-type primary cortical neurons, I was able to demonstrate by fluorescence microscopy, that the morphology of somato-dendritic compartment is strongly altered. Then, using MPS III-EVs isolated from MPS IIIB brains of mice aged of 1 month, 4 months and 8 months, I showed that EVs induced a different transcriptional signature of neuron depending on the stages of the disease. To summarize, these results strongly suggest that in patients with Sanfilippo syndrome, EVs actively participate to the neuron pathology and dysfunction, certainly through the spreading of inflammation and the alteration of neurotransmission mechanisms. My PhD work is the first report on the content of EVs released by MPS III microglia and reveals a disease-associated signature, providing a framework for future studies on biomarkers to evaluate efficiency of emerging therapies
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Yioulatos, Denis. "Maitrise de l'espace et accès aux ressources chez le singe hurleur roux (alouatta seniculus) de la Guyane française : Etude morpho-fonctionnelle." Paris, Muséum national d'histoire naturelle, 1994. http://www.theses.fr/1994MNHN0014.
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Cette étude tente d'établir des associations entre la forme et la fonction de certains caractères osteo-musculaires, ainsi que leur importance au cours des mouvements des membres pendant la locomotion et les postures d'un primate platyrrhinien alouatta seniculus. Il a été procédé a une description osteo-musculaire qualitative et quantitative, un inventaire détaille de son registre locomoteur et postural dans une forêt primaire en Guyane française, et une analyse cinématique des principaux modes de locomotion. A. Seniculus exploite les hautes strates de la canopée utilisant principalement des supports moyens et petits, qui sont horizontaux et modérément inclines. Les principaux modes locomoteurs sont le grimper dans toutes les directions, la marche arboricole et la formation des ponts. Les postures d'alimentation dominantes sont la position assise et la suspension par la queue et les deux membres postérieurs. Par ailleurs, la queue préhensile est modérément utilisée au cours de la locomotion, mais elle est largement utilisée pendant l'alimentation. Le membre antérieur est très mobile au niveau de l'épaule permettant des mouvements amples et facilitant l'exploitation des supports fragiles et instables pendant le déplacement et l'alimentation. Cependant, la stabilité des articulations distales contribuerait à l'utilisation des supports plus stables. Au membre postérieur, toutes les articulations sont très mobiles ce qui favorise des orientations diverses, et permet l'exploitation des supports fragiles et instables. Enfin, la queue préhensile semble contrôler le mouvement, et stabiliser et supporter le poids corporel facilitant l'exploitation des supports fragiles
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Callender, Ronald Montgomery. "Hurter and Driffield: the critical years, 1889 to 1898." Thesis, University of Bolton, 2004. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.748547.
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Milot, Emmanuel. "Dispersion et génétique chez un oiseau marin longévif : l'albatros hurleur : dynamique de population, structure et diversité génétiques, consanguinité." Thesis, Université Laval, 2009. http://www.theses.ulaval.ca/2009/26456/26456.pdf.
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L’impact écologique et évolutif de la dispersion et de la consanguinité peut être exacerbé chez les espèces insulaires. Les albatros, en particulier, ont un mode de vie exceptionnel qui soulève plusieurs questions à cet égard. Dans cette thèse j’aborde certaines de ces questions. En introduction (chapitre 1), j’énonce des hypothèses spécifiques à l’espèce d’étude, l’Albatros hurleur (Diomedea exulans), en lien avec la dispersion, la dynamique des populations, et la génétique. Cependant, la découverte fortuite d’une diversité génétique très pauvre chez cette espèce a nécessité une redéfinition majeure des objectifs et hypothèses initiaux de la thèse. Donc, les patrons de diversité génétique chez deux espèces « sœurs », les Albatros hurleurs et d’Amsterdam (D. amsterdamensis), sont d’abord étudiés au chapitre 2. Des simulations supportent l’hypothèse voulant que ces deux espèces aient hérité leur faible diversité de leur ancêtre commun qui vivait il y a quelque 0,8 million d’années. Par conséquent, les albatros semblent défier l’opinion répandue voulant qu’une faible diversité génétique réduit nécessairement la viabilité d’une espèce. L’objectif du chapitre 3 était d’identifier explicitement le modèle de dynamique populationnelle correspondant le mieux à la réalité des Albatros hurleurs. Les populations de l’ensemble de l’aire de répartition s’avèrent très peu différenciées au plan génétique. Tous les génotypes forment un seul groupe homogène selon une analyse de groupement, suggérant que les colonies actuelles pourraient descendre d’une même population ancestrale qui avait une faible diversité génétique. À l’opposé, les données de relecture de bagues indiquent qu’environ un oiseau par cohorte a émigré de son île natale au cours des dernières décennies. De ce fait, les données génétiques ne reflètent pas les faibles taux de dispersion contemporains, vraisemblablement parce que les populations n’ont pas atteint l’équilibre migration–dérive. Un modèle de dynamique de métapopulation impliquant la colonisation récente de plusieurs îles semble compatible avec les bas niveaux de diversité et de structure génétiques, bien que d’autres facteurs ont sans doute contribué au façonnement du patron génétique. La diversité et la structure génétiques limitées soulèvent des questions à propos de la consanguinité et de ses effets. Dans le chapitre 4, les données probantes relatives à la consanguinité chez l’Albatros hurleur sont passées en revue. L’hypothèse que le succès reproducteur décroît avec l’accroissement de la similarité génétique des partenaires a aussi été testée en utilisant des données moléculaires et sur l’histoire reproductive des couples. Bien que l’analyse ne supporte pas cette hypothèse, on ne peut exclure la possibilité que ce résultat découle du manque de résolution des marqueurs étant donné la très faible diversité génétique des albatros. Quelques perspectives sur des aspects reliés à la consanguinité (p. ex. son évitement, la purge génétique), basées sur la littérature récente, sont également proposées. En somme, l’étude du cas « albatros » mène à plusieurs hypothèses stimulantes et démontre la complexité de mettre au jour la dynamique de la consanguinité chez les espèces longévives. Dans le chapitre 5, à défaut de pouvoir faire des analyses d’assignation populationnelle (en raison du manque de résolution génétique), le jeu de données sur les albatros, ainsi qu’un second jeu sur le Saumon Atlantique (Salmo salar), ont servi à explorer le comportement d’une méthode d’assignation appliquée de façon routinière en biologie. Les résultats démontrent que des aspects importants (estimation du taux d’erreur, détection de migrants) sont liés à la conformité des données empiriques aux prémisses des tests. Ils soulignent aussi l’importance de valider la procédure d’assignation à l’aide de simulations préliminaires. Cette contribution méthologique se veut en quelque sorte une réponse au manque d’uniformité dans l’application de ces méthodes. Pour conclure, je passe en revue l’ensemble des connaissances sur la dispersion des Albatros hurleurs et je propose une perspective sur les causes de la dispersion et de son évolution chez ces oiseaux. Cette thèse apporte un éclairage nouveau sur la signification et l’impact du monomorphisme génétique dans les populations naturelles, sur la dispersion et la dynamique populationnelle chez une espèce longévive, et propose une vision sur l’interaction entre ces facteurs et l’histoire de vie.<br>The ecological and evolutionary impact of dispersal and inbreeding may be exacerbated in insular species. Albatrosses, in particular, have an extreme way of life raising several questions in that regard. In this thesis, I address some of these questions. In the introduction (chapter 1), I enounce hypotheses that are specific to the study species, the wandering albatross (Diomedea exulans), in relation to dispersal, population dynamics, and genetics. However, the fortuitous discovery of a very poor genetic diversity in this species led to substantial modifications of the initial objectives and hypotheses of the thesis. Thus, the patterns of genetic diversity in two sister species, the wandering and Amsterdam (D. amsterdamensis) albatrosses, are studied in chapter 2. Simulations support the hypothesis that the two species inherited a poor genetic diversity from their common ancestor, some 0.8 million years ago. Albatrosses thus appear to challenge the widespread view about the negative consequences of genetic depletion on species survival. In chapter 3, the objective was to identify explicitly which model of population dynamics best applies to the wandering albatross. Populations exhibited little genetic differentiation across the species’ range. All genotypes grouped together in a cluster analysis, suggesting that current colonies have derived from one ancestral source that had a low genetic diversity. In contrast, band re-sighting data indicated that about one bird per cohort has dispersed among islands in the past decades. Therefore, low contemporary dispersal rates are not mirrored by genetic data, presumably because populations are not at migration–drift equilibrium. A metapopulation dynamics model involving the recent colonization of several islands seems consistent with the very low levels of both genetic diversity and structure within the wandering albatross. Yet, other factors likely contributed to shape current genetic patterns. The limited genetic diversity and structure raise questions about inbreeding and its effect. Thus, in chapter 4, evidence for inbreeding in the wandering albatross is reviewed. The hypothesis that reproductive success decreases with increasing genetic similarity between mates was also tested using molecular data and pair breeding histories. While the hypothesis was not supported, a lack of resolution from the markers cannot be ruled out given the very poor genetic diversity in albatrosses. Some perspectives about inbreeding-related aspects (e.g. inbreeding avoidance, purging) based on recent literature are also proposed. Overall, this wandering albatross case study leads to several stimulating hypotheses and shows how complex the understanding of inbreeding dynamics in a long-lived species may be. In chapter 5, failing to successfully apply population assignment methods (because of the lack of genetic resolution), data on Atlantic salmon (Salmo salar) were used in addition to the albatross dataset to explore the performance of an assignment method routinely used in biological investigations. Results show that critical aspects (error rate estimation, migrant detection) relate to how test assumptions are met by empirical data. They also stress the need to validate the assignment procedure with preliminary simulations. This methodological contribution is to some extent a response to the absence of uniformity in the way these methods are generally applied. To conclude, using empirical evidence on dispersal in wandering albatrosses, I suggest perspectives on the causes and the evolution of dispersal in these birds. This dissertation provides new insights about the significance and implications of genetic monomorphim in natural populations, about dispersal and population dynamics in a longlived seabird, and proposes a vision about the interaction between these factors and life history.
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Hurley, Shawn Patrick. "Expression profiling and function elucidation of anaplastic lymphoma kinase in the developing nervous system." Diss., Montana State University, 2006. http://etd.lib.montana.edu/etd/2006/hurley/HurleyS0506.pdf.
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Hurley, Sarah. "Factors affecting long-term outcomes following intensive in-home services." View the abstract Download the full-text PDF version (on campus access only), 2008. http://etd.utmem.edu/ABSTRACTS/2008-050-Hurley-index.htm.
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Thesis (Ph.D.)--University of Tennessee Health Science Center, 2008.<br>Title from title page screen (viewed on February 18, 2008). Research advisor: Teresa M. Waters, Ph.D. Document formatted into pages (viii, 88 p. : ill.). Vita. Abstract. Includes bibliographical references (p. 67-79).
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LEQUETTE, BENOIT. "Formation du couple et succes reproducteur chez l'albatros hurleur (diomedea exulans)." Rennes 1, 1992. http://www.theses.fr/1992REN10014.
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Ce travail s'integre dans l'etude des liens existant entre les mecanismes de formation des couples et la qualite des reproductions a suivre. Nous nous sommes tout d'abord attaches a decrire avec precision les signaux visuels et acoustiques de la parade nuptiale de l'albatros hurleur diomedea exulans (oiseaux, procellariiformes, diomedeidae) afin d'en comprendre les fonctions. La plupart de nos releves ont ete effectues a l'ile de la possession dans l'archipel des crozet (terres australes et antarctiques francaises). Nous avons ensuite effectue des comparaisons avec les autres especes de la famille des albatros pour essayer de determiner l'origine de la diversite des signaux utilises. Cette etude comparative nous a egalement permis d'aborder le role de la parade dans les fonctions de reconnaissance specifique, sexuelle et individuelle. Nous nous sommes ensuite interesse aux liens pouvant exister entre la parade nuptiale et la recherche du partenaire au sein de sa propre espece. Chez les especes monogames et longevives, les caracteres les plus susceptibles d'etre pris en compte par les oiseaux sont a priori des caracteres 1) dont depend la qualite des reproductions a suivre, et 2) qui varient entre les individus. Nous avons donc recherche les liens existant entre les caracteristiques individuelles et le succes reproducteur, a partir de nos propres observations et de comparaisons avec d'autres especes d'albatros et d'oiseaux. Le succes reproducteur de l'albatros hurleur variait assez peu en fonction de l'age et de l'experience des individus, deux caracteres consideres comme primordiaux chez les oiseaux. De meme, la duree des liens du couple semble avoir peu d'importance sur le succes reproducteur. Lors de la recherche d'un conjoint, l'age du partenaire potentiel semblait etre un des premiers caracteres pris en compte par les oiseaux. Ce critere semblait pouvoir etre estime de maniere active. Nous avons donc cherche a comprendre quelle pouvait etre la consequence d'un tel choix sur les reproductions a suivre. Suite a des comparaisons avec d'autres especes d'albatros et d'autres oiseaux marins, nous avons conclu que cet aspect de la biologie de l'albatros hurleur pouvait etre representatif d'autres especes monogames et longevives
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Devineau, Nicolas Olivier Christophe. "Evolution de la loi Huriet." [S.l.] : [s.n.], 2005. http://theses.univ-nantes.fr/thesemed/PHdevineau.pdf.
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Günther, Edeltraud, and Lilly Scheibe. "The Hurdles Analysis : A method to identify and analyse hurdles for green procurement in municipalities." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2003. http://nbn-resolving.de/urn:nbn:de:swb:14-1074594203546-41307.
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Public procurement amounts to approximately 12% of the gross domestic product of industrialized countries. The greatest part of it is controlled by municipalities. Consequently, their procurement decisions have an enormous potential to initiate and support sustainable development. But measures that have been taken so far did not have a substantial impact on production processes and products yet. This was the starting point for heading toward one of the objectives of the European research project RELIEF (Environmental Relief Potential of Urban Action on Avoidance and Detoxification of Waste Streams through Green Public Procurement): to identify existing hurdles for green public procurement and to develop strategies for overcoming those hurdles. For this a decision-oriented view on public procurement was chosen, possible hurdles, revealed in practice, were identified with the help of a therefore developed questionnaire and their relevance for the cities participating in the RELIEF project was examined. Thus the concept of the hurdles analysis was developed. Subsequently the method was improved by expanding the questioning on other European municipalities than those taking part in the RELIEF project as well as using it within the German research project NaBesI where a case study was accomplished. Based on the experiences and outcomes of these first hurdles analyses a self-evaluation tool was developed. It shall enable municipalities to assess their hurdles for green procurement and deduce strategies on their own.
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Ingebretsen, Sarah. "Steeplechase Hurdle Economy, Mechanics, and Performance." BYU ScholarsArchive, 2013. https://scholarsarchive.byu.edu/etd/3631.
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Research surrounding the steeplechase is scarce, with most research focusing primarily on how biomechanical factors relate to maintaining running speed while crossing barriers. One area that has not been well explored is the relationship between biomechanical factors and hurdling economy. The purpose of this study was to investigate how performance times and biomechanical variables relate to hurdling economy during the steeplechase. This was accomplished by measuring running economy of collegiate and professional steeplechasers while running with and without hurdles. Biomechanical measures of approach velocity, take-off distance, clearance height, and lead knee extension while hurdling, as well as steeplechase performance times were correlated to a ratio of running economy with and without hurdles. Results indicated no correlation between steeplechase performance time and the ratio of running economy during the hurdle and non-hurdle laps. Results also indicated no correlation between the aforementioned biomechanical variables and ratio of running economy during the hurdle and non-hurdle laps. Increasing approach velocity did not negatively affect running economy. Steeplechasers may continue to increase approach velocity without hurting their economy or performance times.
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Wagner, Béatrice. "Les tumeurs à cellules de Hürthle : à propos de 12 cas et revue de la littérature." Université Louis Pasteur (Strasbourg) (1971-2008), 1987. http://www.theses.fr/1987STR1M296.
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Pasqual, Nicolò <1992>. "Italian wine in China, hurdles and potentialities." Master's Degree Thesis, Università Ca' Foscari Venezia, 2017. http://hdl.handle.net/10579/11636.
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I became interested in this topic while I was in China. When I had the chance to meet a Chinese wine distributor was astonished when I realized that among the numerous bottles of French wine there was almost no Italian one. That was a clear indicator of the situation of Italian wine in China. So, I started researching why an important wine producing country like Italy wasn’t able to replicate in China the success it has worldwide. During my research, I became more and more aware of the potentialities of the Italian wine and how there were ill-used during the first twenty years China opened its market to foreign wines. While the French wineries entered the market almost immediately and started promoting their wines, Italian wineries decided not to give importance to this market. Now they are still paying the price, in 2016 Italy had only 5% of the total wine imported market share while France had 44% of it.
The research has been divided into three parts: the first part will give a global presentation of the wine industry focusing on Italy as wine exporting country and on the Chinese market attractivity. The second part will present the choice of internationalization, especially for small and medium wineries, the biggest obstacles and the possible solutions. The last part is the result of the interviews I conducted with the people working in the sector, Italian and Chinese, the major problems they encountered and their insights about how to change the current situations.
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Mohamed, Yacoub. "AFRICOM an effective organization or a military hurdle?" Thesis, Monterey, California. Naval Postgraduate School, 2010. http://hdl.handle.net/10945/4993.
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Approved for public release; distribution is unlimited<br>Many organizations face challenges that impact their effectiveness and therefore success. These challenges are not easy to visualize, thus making it more difficult to derive and implement appropriate measures to deal with them. The problem becomes compounded when procedures or treatments are applied without diagnosis. The establishment of United States Africa Command (AFRICOM) to serve Africa's unique security concerns generated some reservations both within and outside the United States (U.S.). Despite attempts to communicate AFRICOM's intentions, the lukewarm acceptance among its stakeholders still persists and has raised concerns about its efficacy as a panacea for the problems on the African continent. The basic premise of this study is to diagnose AFRICOM's internal processes and examine its external environment in order to identify any organizational or environmental variables that support or hinder its effectiveness in attaining its goals. This thesis examines the extent to which key variables, such as mission, tasks, structure, culture, and environment are congruent or incongruent. Data was obtained both qualitatively and quantitatively. This study establishes that there is some degree of misalignment among the inputs, outputs, and the desired outcomes of AFRICOM's programs, and proffers recommendations for a better fit.
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Rodriguez, Leon Alexis, and Khushboo Singh. "Adoption hurdles faced by organizations embracing SAFe®." Thesis, Uppsala universitet, Industriell teknik, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-411177.
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Agile methodology has gained wide adoption. Agile methodology, with its iterative and incremental approach for software development, has gained popularity within IT organizations that are facing a dynamic business environment. Agile software development has emerged as an alternative to the traditional Waterfall model in delivering software at a faster pace and having more receptiveness towards the changing requirements. However, Agile methodology was originally designed for small and individual teams. This makes moving Agile to large-scale organizations a complicated task. In this thesis, we will review the challenges and success factors presented by (Dikert et al., 2016) in their systematic literature review for SAFe (Scaled Agile framework). Our thesis is based on empirical research. The authors have conducted interviews to gather data to underpin or dissent from the findings in the systematic literature review regarding the challenges and success factors of implementing SAFe in organizations.
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Provost, David 1959. "Hurdles in the business case for the Semantic Web." Thesis, Massachusetts Institute of Technology, 2004. http://hdl.handle.net/1721.1/17883.
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Thesis (S.M.M.O.T.)--Massachusetts Institute of Technology, Sloan School of Management, Management of Technology Program, 2004.<br>Includes bibliographical references (leaves 29-34).<br>The nuclear winter that filled the vacuum created by the Internet implosion was characterized by highly conservative investments in new technologies. This was particularly true for Internet- and Web-oriented technologies since after all, being a believer just wasn't as popular as it used to be. However, life, business, and science go on, and the Web is no exception. This thesis will examine hurdles in the business case for the Semantic Web. In one sense, the Semantic Web is an extension or enhancement of the existing World Wide Web (Web). As we know it today, the Web is a rich medium that allows humans to express themselves, learn, interact, and reach an audience that was a pipe dream just a decade ago. At the same time, the Web is of limited utility to computers (machines). For example, a human being could easily recognize a postal address or the specifications of an order for steel; a machine could not. To a machine, these data would simply be elements to be rendered and displayed on a monitor, with no intrinsic or cumulative meaning. In this sense, one of the goals set for the Semantic Web is to create meaning and utility for machines that allows for interpretation and action with far less human intervention. Issues related to the challenges, practicalities, theories and opportunities of the Semantic Web will be discussed. In the process, hopefully, this thesis will identify some of the stepping stones in building a business case for this evolution. Notably, today's comments regarding the Semantic Web sound very similar to what was once said about the practicalities of eBusiness and the likelihood of its adoption.<br>by David Provost.<br>S.M.M.O.T.
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Van, Harte MA. "Development of regional reliability hurdle rate for South Africa." Master's thesis, University of Cape Town, 2008. http://hdl.handle.net/11427/5094.
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The reform of the electricity distribution industry has resulted in the national regulator authorities moving from Rate of Return (ROR) to Incentive Based Regulation (IBR) quality regulation mechanism. This has influenced the reporting, planning, design, operating and maintenance (O&M) philosophy surrounding distribution networks and service delivery to the end customer. The introduction of IBR regulation has created a need to understand how the IBR scheme implemented will influence the capital and operating investment decisions. The aim of the work leading up to this thesis has been to develop reliability hurdle rates that include the effect of the IBR scheme implemented by the national regulator authority of South Africa. This will enable the network planner to compare the investment decision with the reward / penalty scheme. The thesis addresses the challenges facing distribution network planning to achieve the appropriate (optimal) balance of investment cost vs. reliability levels. Value Based Reliability Planning (VBRP) methodology is an approach to evaluate capital investment decisions by quantifying, in economic terms, the benefits of improving reliability (System Average Interruption Duration Index (SAIDI) per capital cost required) by comparing different reliability improvement alternatives. This is achieved by relating the investment decision to the reward / penalty scheme introduced by National Electricity Regulator for South Africa (NERSA). To reach this goal this thesis derived a reliability hurdle rate by utilising benefit-to-cost analysis principles by considering the shape of the reward / penalty scheme. Furthermore, this necessitates the determination of the optimal balance between investment decisions to obtain improvement in the continuity of supply indices. One of the fundamental objectives is therefore to relate the IBR and VBRP in an efficient and effective way, that is the aim of the national regulator authority. A new method to determine a regional and national reliability hurdle rate is proposed by the thesis. This is a first step in obtaining an optimal expansion alternative and allows the planner to compare the preferred alternative selected against a hurdle rate. Since the method considers the reward / penalty scheme, the thesis first reviews the principles that influence the quality regulation mechanisms and the benefit-to-cost analysis techniques. The second step demonstrates the derivation of the reliability hurdle rate utilising the reward / penalty scheme adopted within South Africa. Parameters considered include the number of customers and the interruption indices in each region. The application studies demonstrate the derivation of the regional reliability hurdle rate from the six regions within South Africa. This allows the derivation of regional reliability hurdle rate from the different regional reward / penalty schemes. Finally, a sensitivity analysis is performed to understand the influence of different parameters, which will influence the regional reliability hurdle rate. The results from the case studies show that, when NERSA creates the reward / penalty scheme, it is crucial that the approved method is applied to determine the shape of the scheme implemented, which will allow the derivation of the reliability hurdle rate. The new method is suitable for implementation where the IBR quality regulation mechanism has been adopted by the national electricity authorities. Furthermore, the reliability hurdle rate derived can be used to compare the investment decision against the improvement required in the continuity of supply indices. This ensures the trade-off between the expansion investment and improvement in the reliability levels
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Cano, Eduardo Fiacadori. "O consumo de microcomputadores no Brasil: uma análise utilizando dados das PNADs de 2001 a 2007 e da POF 2002-2003." Universidade de São Paulo, 2010. http://www.teses.usp.br/teses/disponiveis/96/96131/tde-05052010-150700/.
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O consumo domiciliar de microcomputadores cresceu de forma acelerado nos últimos anos. Apesar de grupos com determinadas características socioeconômicas terem um consumo maior de computadores, o consumo vem aumentando em todos os grupos. Ou seja, mesmo com diferenças grandes, o consumo de computadores não é mais uma exclusividade dos mais ricos, dos mais bem instruídos ou de determinadas regiões do país. Os modelos Probit e double-hurdle se mostraram adequados para analisar o consumo de computadores no Brasil. O modelo Probit ajustou bem os dados na análise da presença de um computador no domicílio. Já para a despesa com computador, o modelo double-hurdle se mostrou melhor que o modelo Tobit, uma vez que este separa a análise do processo de decisão de compra em dois, separando a decisão de gastar da decisão de quanto gastar.<br>Household consumption of microcomputers grew up fast in recent years. Despite some socioeconomic groups take highercomputer consumption, consumption is increasing in all groups. In other words, even with large differences, the consumption of computers is no longer uniqueness of wealthier, better educated or at certain regions of the country. Probit and double-hurdle models were suitable for analyzing the consume of computers in Brazil. The Probit model adjusted well the data set in the analysis of the presence of a computer at home. For the computer spending the double-hurdle model proved better than Tobit because separates the analysis of the purchase decision process in two, separating the decision to spend from the decision of how much spend.
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Barrón, Manuel. "Exclusion and Discrimination as Sources of Inter-Ethnic Inequality in Peru." Economía, 2012. http://repositorio.pucp.edu.pe/index/handle/123456789/117536.
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According to the 2003 National Household Survey, mean labour income for an indigenous worker is only 56% of that for a non-indigenous worker. Studies of ethnic discrimination in Peru’s labour markets generally find that discrimination is too low to explain inequalities of this magnitude. However, Sigma Theory (Figueroa 2003) predicts that social exclusion is a source of inter-ethnic inequality, and that has not been empirically tested. The primary aim of this paper is to fill this gap by estimating the extent to which exclusion and discrimination contribute to income inequality. Hurdle models are used to tackle down econometric endogeneity of years of schooling and truncation-at-zero of incomes. The results imply that exclusion plays a stronger role on inequality than discrimination: without exclusion, the Gini of labour income would decrease from 0.64 to 0.45, and without discrimination it would be reduced to 0.50.<br>De acuerdo a la Enaho 2003, el ingreso promedio de un trabajador indígena es solo 56% del de un trabajador no-indígena. Sin embargo, estudios sobre discriminación étnica en los mercados laborales de Perú usualmente hallan brechas demasiado pequeñas como para explicar la desigualdad observada. De acuerdo a Figueroa (2003), la exclusión social es una fuente importante de desigualdad interétnica, pero esto no ha sido contrastado empíricamente. El objetivo central de este documento es llenar esa brecha estimando qué porcentaje de la desigualdad se debe a exclusión y qué porcentaje a discriminación, comparando directamente los efectos. La metodología econométrica utilizada (hurdle models) permite incluir en el análisis a los trabajadores con ingresos nulos y contrarrestar problemas de endogeneidad econométrica. Los resultados implican que la exclusión juega un papel más importante que la discriminación. Sin exclusión, el Gini de ingresos laborales se reduciría de 0.64 a cerca de 0.45; sin discriminación, a alrededor de 0.50.
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Thünen, Torsten [Verfasser], Thomas [Akademischer Betreuer] Hurek, Barbara [Akademischer Betreuer] Reinhold-Hurek, and Rudolf [Akademischer Betreuer] Aman. "Erfassung der mikrobiellen Diversität in verschiedenen Reisarten und Varietäten / Torsten Thünen. Gutachter: Barbara Reinhold-Hurek ; Rudolf Aman. Betreuer: Thomas Hurek." Bremen : Staats- und Universitätsbibliothek Bremen, 2005. http://d-nb.info/1072302209/34.
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Ibarz, Mercè. "Buñuel Documental. "Las Hurdes, Tierra sin Pan" i el seu Temps." Doctoral thesis, Universitat Pompeu Fabra, 1997. http://hdl.handle.net/10803/7524.
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LA TESIS INVESTIGA Y ANALIZA EL PROCESO DE CREACION Y DE RECEPCION DEL FILME DOCUMENTAL DE LUIS BUÑEL SOBRE LAS HURDES, REALIZADO EN 1933. LA INVESTIGACION ARRANCA CON LOS PROCEDENTES HISTORICOS, METAFORICOS Y VISUALES DE LA OBRA, QUE SE ENCUENTRAN EN LOS VIAJES ANTERIORES DE MIGUEL DE UNAMUNO Y, EN PARTICULAR, DE ALFONSO XIII Y EN EL FOTOPERIODISMO QUE LA VISITA REAL PROVOCO, ASI COMO EN EL ESTUDIO DE GEOGRAFIA HUMANA DE MAURICE LEGENDRE, EN EL QUE BUÑUEL SE INSPIRO ABIERTAMENTE. LA INVESTIGACION PODE DE RELIEVE EL CARACTER ANARCO-SURREALISTA DEL FILME, TANTO EN VIRTUD DEL EQUIPO DE PRODUCCION Y FILMACION QUE LO REALIZO, COMO DE SU PUESTA EN ESCENA. SE ANALIZAN LAS TRAYECTORIAS INTELECTUALES, ARTISTICAS Y POLITICAS DE CADA MIEMBRO DEL EQUIPO, QUE DIERON COMO RESULTADO UN FILME PLURIDISCIPLINARIO DE ESPECTRO CULTURAL AMPLIO: CINEMA, FOTOGRAFIA, POESIA, PERIODISMO, ETNOGRAFIA, HISTORIA DEL ARTE. ASIMISMO, LA TESIS SE DETIENE EN LA DESCRIPCION Y ANALISIS DE LA PUESTA EN ESCENA, EN PARTICULAR DE LAS ESCENAS FABRICADAS. EL ANALISIS DE LA RECEPCION DEL FILME EN LAS DIVERSAS ETAPAS DE LA II REPUBLICA Y DE LA GUERRA CIVIL, ASI COMO DESDE ENTONCES HASTA HOY, CIERRA LA TESIS.
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McNairney, Fiona. "Using images to overcome communication hurdles posed by foreign site operatives." Thesis, Glasgow Caledonian University, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.572806.
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Despite the economic recession there is still demand for foreign labour in the UK construction industry. This presents many construction management problems, not least is the communication barrier. Some research examining the extent of the language barrier within the construction industry has been undertaken. Several of these studies have advocated the use of images as a means of communicating with site operatives who have little English. Other studies have actively created communication material for use in site health and safety communications. The author's research contributes to this existing body of research by exerting a stronger focus on the source -channel - delivery. Furthermore, the research was unique in that it addressed the contribution that the presenter of the communication exerted on the audience, in terms of knowledge gain and perception. The aims of the research were: to determine how images currently used within the industry are understood by site operatives of varying cultural background and language proficiency; ~ how comprehension of site specific information following an induction using images compares to inductions without images; and how the presenter of site inductions can influence audience perception and knowledge gain. An exploration into the nature and extent of the communication problem was conducted via interviews and site visits. A series of tests were then developed and piloted before '? conducting preliminary comprehension testing. Observations of site inductions were then undertaken to assess presenter credibility. Feedback from site operatives was elicited using a likert scale. Finally, knowledge gain was assessed by asking operatives open questions regarding site specific information. Qualitative analysis highlighted that health and safety images currently in use are often misinterpreted by non English speaking operatives, but, simple design changes can drastically alter comprehension toward the intended meaning. The credibility assessment method indicated that it was sensitive enough to differentiate between differences in credibility, but further development of the instrument is required. The final stage of analysis revealed that more information was transmitted to receivers when using pictorial inductions compared with text based inductions.
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Li, Rui. "OVERCOMING INITIAL HURDLES: STRATEGIES FOR DEVELOPING A UNIVERSITY FREE IMPROVISATION ENSEMBLE." UKnowledge, 2015. http://uknowledge.uky.edu/music_etds/52.
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New free improvisers may come across six major problems as they learn to improvise: imbalance between technique and music, incorrect perception of limitation, imbalance between rationality and emotion, lack of enthusiasm, inability to view criticism as a source of creativity, and misunderstanding of mistakes and risks. In this thesis, I propose a set of effective pedagogical tools as possible solutions for students and groups interested in exploring the beauty of free improvisation.
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Bollschweiler, Laurence R. "A biomechanical analysis of male and female intermediate hurdlers and steeplechasers /." Diss., CLICK HERE for online access, 2008. http://contentdm.lib.byu.edu/ETD/image/etd2254.pdf.
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Duncan, Melanie Laura. "The Final Hurdle: The role of envelope proteins in poxvirus release." Thesis, The University of Sydney, 2021. https://hdl.handle.net/2123/25858.
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Increasing incidence of antimicrobial resistance requires identification of novel drugs, and the human genome presents thousands of potential targets. Intracellular pathogens rely on host proteins and pathways during their entry, replication and egress.
Poxviruses are reliant on host Abl tyrosine kinases for release of enveloped virus, the last step in their egress. Release can be inhibited by Abl kinase targeting drug imatinib, with treatment shown to rescue mice infected with a lethal dose of vaccinia virus (VACV). It is speculated that host-targeted antimicrobials are less likely to select for resistance. This thesis uses VACV as a model to study the effects of treatment by a host- targeted antimicrobial.
Following treatment by imatinib, a mutation in VACV outer envelope protein A34 was identified that elicits high viral release. This study characterises the novel A34 mutation alongside published mutations in envelope proteins A34 and B5, which each lead to higher virus release from cells than the parental strain. Mutations in A34 demonstrate resistance to treatment with imatinib, suggesting that antimicrobial resistance may still arise against host-targeted antimicrobials via compensatory mutations in parallel pathways. This thesis further explores the potential mechanisms of viral release by expressing the A34 ectodomain for structural analysis.
The biological significance of release of enveloped virus during infection is an open question. Ectromelia virus (ECTV) provides a model of disease in a native murine host. Actin nucleation driving enveloped virus out of cells prior to release is highly conserved among poxviruses. Actin nucleation has been mapped to two tyrosine residues on envelope protein A36 in VACV. Here, the ECTV A36 homologue is examined and actin nucleation mapped to phosphorylation of a single tyrosine. Further, while actin-based motility is shown to play a critical role in the release of ECTV from infected cells, its loss was not critical in vivo.
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Bollschweiler, Laurence R. "A Biomechanical Analysis of Male and Female Intermediate Hurdlers and Steeplechasers." BYU ScholarsArchive, 2007. https://scholarsarchive.byu.edu/etd/1233.
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In the sport of track and field, proper hurdling technique is a complicated combination of various running and jumping kinematics. With most research having been done on sprint hurdling, there is a growing need for research on hurdling events of different lengths. The intermediate hurdles (IH) and the steeplechase (SC) are two events where there are a number of differences in hurdling technique. This study compared the differences in hurdling technique between events (IH and SC) as well as the differences in technique between genders. Subjects for this study consisted of 20 elite intermediate hurdlers (10 male, 10 female) and 20 elite steeplechasers (10 male, 10 female). Subjects were filmed performing their respective events at the 2006 USA Outdoor Track and Field National Championships. A 2-D analysis was performed on each subject to determine differences between events and genders for the following variables: loss of horizontal velocity, peak center of mass relative to hurdle height, horizontal position at peak center of mass, deviation angle at takeoff, hurdle step length, penultimate, and recovery step lengths, takeoff and landing distance, minimum lead leg hip angle, trail leg knee height relative to the hip at peak height, trunk angle at peak height, landing angle, and finally, the ratio of the recovery step to the penultimate step. Significant differences (p < .05) were observed in 11 of the 13 variables analyzed. Steeplechasers showed significantly higher values than hurdlers in deviation angle, landing angle, minimum lead leg hip angle, peak height over the barrier, takeoff and landing distances, as well as penultimate, hurdle and recovery step lengths. Trail leg knee height was shown to be higher for hurdlers. Also, female steeplechasers were shown to have a greater trunk angle and loss of horizontal velocity than female hurdlers. Females showed higher values than males in deviation angle, landing angle, minimum lead leg hip angle, and peak height over the barrier. Landing distance, hurdle step length and trail leg knee height were higher for males. Also, female steeplechasers had a longer penultimate step length than males. Several differences in hurdling technique exist between events and gender. Hurdlers appear to place more emphasis on the kinematics which helps to promote a low center of mass hurdle clearance. Steeplechasers, on the other hand, are less pronounced with their hurdling kinematics. This is likely due to the greater economy required of the longer event. Gender differences appear to be, in large part, a function of differences in barrier height. As athletes and coaches go about evaluating and training hurdling technique, it is important to recognize the differences that exist between these different events.
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Julliot, Catherine. "Utilisation des ressources alimentaires par le singe hurleur roux, alouatta seniculus (atelidae, primates) en Guyane : impact de la dissémination des graines sur la regénération forestière." Tours, 1992. http://www.theses.fr/1992TOUR4010.
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Ce travail consiste en l'étude des relations entre les singes hurleurs roux, (alouatta seniculus) et les plantes qui leur sont associées, afin de déterminer l'impact de ce primate sur la regénération de la forêt tropicale primaire. Les variations saisonnières de l'exploitation de l'espace, des rythmes d'activité et du régime alimentaire des singes hurleurs sont étroitement liées à la disponibilité des ressources alimentaires dans le milieu, et plus particulièrement des ressources en fruits. Bien qu'étant les primates les plus folivores d'Amérique du sud, les singes hurleurs consomment une part non négligeable de fruits et sont, de plus, des disséminateurs de graines efficaces. En effet, ils disséminent par endozoochorie la quasi-totalité des espèces végétales dont ils consomment les fruits sans affecter le pouvoir de germination des graines. Mais surtout, les hurleurs défèquent régulièrement au niveau de leurs dortoirs. Cette concentration de graines d'espèces exploitées par les singes hurleurs sur des sites particuliers modifie le potentiel de regénération de la forêt de façon très localisée, contribuant ainsi à l'hétérogénéité spatiale de la forêt.
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Olsson, Mats-Olov. "Barriers to change? : understanding the institutional hurdles in the Russian forest sector." Licentiate thesis, Luleå, 2004. http://epubl.luth.se/1402-1757/2004/79/index.html.
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McMahon, Carol Martha Mary. "Pathogen control in sous vide processing by thermal inactivation and hurdle technology." Thesis, University of Ulster, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.388950.
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Salo, Aki Iikka Tapio. "An assessment of video motion analysis : variability, reliability, camera orientation and extrapolation." Thesis, University of Exeter, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.286552.
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CROIZAT-VIALLET, CHRISTOPHE. "La loi huret-serusclat et les personnes concernees par la recherche biomedicale." Reims, 1994. http://www.theses.fr/1994REIMM019.
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DARME, XAVIER. "Mise en application de la loi huriet sur la pharmacie hospitaliere : implications pour l'hopital central et l'hopital saint-julien du chu nancy. analyse du role du pharmacien. documents et procedures." Nancy 1, 1993. http://www.theses.fr/1993NAN1A208.
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BARRIAL, GATT CHRISTINE. "Mise en application de la loi huriet-serusclat dans le domaine de la recherche clinique a marseille." Aix-Marseille 2, 1993. http://www.theses.fr/1993AIX20184.
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Lau, Leung-che Miriam. "Passing three hurdles representations of Henrik Ibsen's Nora in twentieth century Chinese theatre /." Click to view the E-thesis via HKUTO, 2009. http://sunzi.lib.hku.hk/hkuto/record/B43958825.
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Lau, Leung-che Miriam, and 劉亮之. "Passing three hurdles: representations of Henrik Ibsen's Nora in twentieth century Chinese theatre." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2009. http://hub.hku.hk/bib/B43958825.
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Martens, Daniel Taylor. "Observational Hurdles in Cosmology: The Impact of Galaxy Physics on Redshift-Space Distortions." The Ohio State University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=osu1523868013598327.
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Bhaktha, Nivedita. "Properties of Hurdle Negative Binomial Models for Zero-Inflated and Overdispersed Count data." The Ohio State University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=osu1543573678017356.
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