Journal articles: 'Hyaline cartilage'

1

Kheir, Ehab, and David Shaw. "Hyaline articular cartilage." Orthopaedics and Trauma 23, no. 6 (December 2009): 450–55. http://dx.doi.org/10.1016/j.mporth.2009.01.003.

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Jeffrey, D. R., and I. Watt. "Imaging hyaline cartilage." British Journal of Radiology 76, no. 911 (November 2003): 777–87. http://dx.doi.org/10.1259/bjr/51504520.

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Engfeldt, BENGT, KJELL Hultenby, and MARTIN MÜLler. "ULTRASTRUCTURE OF HYALINE CARTILAGE." Acta Pathologica Microbiologica Scandinavica Series A :Pathology 94A, no. 1-6 (August 15, 2009): 313–23. http://dx.doi.org/10.1111/j.1699-0463.1986.tb03000.x.

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ENGFELDT, BENGT, BRUCE CATERSON, OLE EKLÖF, KJELL HULTENBY, and MARTIN MÜLLER. "ULTRASTRUCTURE OF HYALINE CARTILAGE." Acta Pathologica Microbiologica Scandinavica Series A :Pathology 95A, no. 1-6 (August 19, 2009): 371–76. http://dx.doi.org/10.1111/j.1699-0463.1987.tb00054_95a.x.

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AL-Mhanna, H. K. N. "Morphological study of the Larynx of the indigenous adult Male Pigeon (Columba domestica)." Al-Qadisiyah Journal of Veterinary Medicine Sciences 12, no. 1 (June 30, 2013): 52. http://dx.doi.org/10.29079/vol12iss1art230.

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Formalistic study elucidate that the larynx in the fourteen healthy indigenous male pigeons (Columba domestica) for benefit in the study of the respiratory physiology, histopathology, and the respiratory diseases analyzes. After bird's preparation, the larynx detected, and then the shape, position and its components studied in details.The larynx emerges in the caudal part of the oropharyngeal cavity as a heart-shaped cartilaginous mass. It composed of a single hyaline cricoid cartilage which consisted of body and left and right wings, double hyaline arytenoid cartilages which consisting of body and rostral and caudal processes, and single hyaline procricoid cartilage which consisted of body dorsally and curved tail caudoventrally. These cartilaginous components surrounded by laryngeal skeletal muscles intrinsic (superficial and deep) and extrinsic (rostral, caudolateral, and caudomedial).

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Hodler, Juerg, Marie-Josée Berthiaume, Mark E. Schweitzer, and Donald Resnick. "Knee Joint Hyaline Cartilage Defects." Journal of Computer Assisted Tomography 16, no. 4 (July 1992): 597–603. http://dx.doi.org/10.1097/00004728-199207000-00020.

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Jonsson, K., K. Buckwalter, M. Helvie, L. Niklason, and W. Martel. "Precision of Hyaline Cartilage Thickness Measurements." Acta Radiologica 33, no. 3 (May 1992): 234–39. http://dx.doi.org/10.1177/028418519203300308.

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Measurement of cartilage thickness in vivo is an important indicator of the status of a joint as the various degenerative and inflammatory arthritides directly affect the condition of the cartilage. In order to assess the precision of thickness measurements of hyaline articular cartilage, we undertook a pilot study using MR imaging, plain radiography, and ultrasonography (US). We measured the cartilage of the hip and knee joints in 10 persons (4 healthy volunteers and 6 patients). The joints in each patient were examined on two separate occasions using each modality. In the hips as well as the knee joints, the most precise measuring method was plain film radiography. For radiographs of the knees obtained in the standing position, the coefficient of variation was 6.5%; in the hips this figure was 6.34%. US of the knees and MR imaging of the hips were the second best modalities in the measurement of cartilage thickness. In addition, MR imaging enabled the most complete visualization of the joint cartilage.

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Fodor, Pal, Arpad Solyon, Raluca Fodor, Cornel Catoi, Flaviu Tabaran, Radu Lacatus, Cristian Trambitas, and Tiberiu Bataga. "Role of the Biomimetic Scaffolds in the Regeneration of Articular Tissue in Deep Osteochondral Defects in a Rabbit Model." Revista de Chimie 69, no. 1 (February 15, 2018): 201–7. http://dx.doi.org/10.37358/rc.18.1.6074.

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The ability of damaged articular cartilage to recover with normal hyaline cartilage is limited. Our aim was to study the mechanism of in vivo cartilage repair in case of severe osteochondral lesions using a three-dimensional matrix implanted without any preseeded cells in the osteochondral defect in a rabbit model. According to the ICRS scores from macroscopic observations of the femoral condyles, the average scores in the scaffold groups were higher than those in the control groups at every time (P[0.001). Histological examination of the ostheochondral defects, revealed regeneration of new tissue with hyaline-like cartilage features only in matrix groups. At twelve weeks from implantation, complete filling of the defect with hyaline cartilage with a tendency of mineralization and the absence of implant material is observed. The superficial area of the defect is completely covered with hyaline-like cartilage. The scaffold used leaded to the regeneration of articular tissue with an ordered histoarchitecture.

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Alcaide-Ruggiero, Lourdes, Verónica Molina-Hernández, María M. Granados, and Juan M. Domínguez. "Main and Minor Types of Collagens in the Articular Cartilage: The Role of Collagens in Repair Tissue Evaluation in Chondral Defects." International Journal of Molecular Sciences 22, no. 24 (December 11, 2021): 13329. http://dx.doi.org/10.3390/ijms222413329.

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Several collagen subtypes have been identified in hyaline articular cartilage. The main and most abundant collagens are type II, IX and XI collagens. The minor and less abundant collagens are type III, IV, V, VI, X, XII, XIV, XVI, XXII, and XXVII collagens. All these collagens have been found to play a key role in healthy cartilage, regardless of whether they are more or less abundant. Additionally, an exhaustive evaluation of collagen fibrils in a repaired cartilage tissue after a chondral lesion is necessary to determine the quality of the repaired tissue and even whether or not this repaired tissue is considered hyaline cartilage. Therefore, this review aims to describe in depth all the collagen types found in the normal articular cartilage structure, and based on this, establish the parameters that allow one to consider a repaired cartilage tissue as a hyaline cartilage.

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Schroeder, Walter A., Margaret H. Cooper, and William H. Friedman. "The Histologic Effect of Hypervitaminosis A on Laryngeal Cartilages." Otolaryngology–Head and Neck Surgery 96, no. 6 (June 1987): 533–37. http://dx.doi.org/10.1177/019459988709600602.

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This study investigated the role of hypervitaminosis A on the developing larynx. Pregnant rats received a dose of 100,000 units of Vitamin A on either Day 8 or Day 11 of gestation. The hyaline laryngeal cartilages of the neonatal rats were studied. The cricoid and arytenoid cartilages appeared to be the most affected. There was a pronounced central disorganization of the structure of the cartilage, with numerous swollen lacunae devoid of chondrocytes. The thyroid cartilage was the least affected. The center of the cartilage displayed a minimal amount of disorganization, when compared to the control. The effect of hypervitaminosis A on cartilaginous tissue is discussed, as well as its possiible effect on the development of laryngeal cartilages.

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AL-Mussawy, A. M. "Anatomical Study of the LarynxIn Indigenous Male Turkey (Meleagris gallopava)." Al-Qadisiyah Journal of Veterinary Medicine Sciences 11, no. 1 (June 28, 2012): 122. http://dx.doi.org/10.29079/vol11iss1art180.

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The study aim to explain the anatomical description of the larynx in the indigenous male turkey (Meleagris gallopava). For making use as a basic information in the study of the respiratory physiology, histopathology, the respiratory diseases diagnosis, and surgery and anesthesia of turkey. Five healthy birds (first year of age and weighing (4715 ± 43.3 gm)) employed in this study. After well bleeding, the larynx detected, and then the shape, position, dimensions of each specimen and its components were recorded. The larynx appeared as a heart-shaped mound in the caudal part of the oropharyngeal cavity. It consisted of a single hyaline cricoid cartilage which consisted of body and left and right wings, double hyaline arytenoid cartilages which consisting of body and rostral and caudal processes, and single hyaline procricoid cartilage which consisted of body dorsally and tail ventrally. Also there were superficial and deep intrinsic and rostral, caudolateral, and caudomedial extrinsic laryngeal skeletal muscles.

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Jonsson, K., K. Buckwalter, M. Helvie, L. Niklason, and W. Martel. "Precision of Hyaline Cartilage Thickness Measurements." Acta Radiologica 33, no. 3 (May 1, 1992): 234–39. http://dx.doi.org/10.3109/02841859209173167.

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13

Trattnig, Siegfried. "Overuse of hyaline cartilage and imaging." European Journal of Radiology 25, no. 3 (November 1997): 188–98. http://dx.doi.org/10.1016/s0720-048x(97)01173-x.

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Jonsson, K., K. Buckwalter, M. Helvie, L. Niklason, and W. Martel. "Precision of Hyaline Cartilage Thickness Measurements." Acta Radiologica 33, no. 3 (January 1992): 234–39. http://dx.doi.org/10.1080/02841859209173167.

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Di Geso, Luca, Marika Tardella, Marwin Gutierrez, Emilio Filippucci, and Walter Grassi. "Crystal Deposition at Elbow Hyaline Cartilage." Journal of Clinical Rheumatology 17, no. 6 (September 2011): 344–45. http://dx.doi.org/10.1097/rhu.0b013e31822e0d36.

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Thorp, Hallie, Kyungsook Kim, Makoto Kondo, Travis Maak, David W. Grainger, and Teruo Okano. "Trends in Articular Cartilage Tissue Engineering: 3D Mesenchymal Stem Cell Sheets as Candidates for Engineered Hyaline-Like Cartilage." Cells 10, no. 3 (March 13, 2021): 643. http://dx.doi.org/10.3390/cells10030643.

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Articular cartilage defects represent an inciting factor for future osteoarthritis (OA) and degenerative joint disease progression. Despite multiple clinically available therapies that succeed in providing short term pain reduction and restoration of limited mobility, current treatments do not reliably regenerate native hyaline cartilage or halt cartilage degeneration at these defect sites. Novel therapeutics aimed at addressing limitations of current clinical cartilage regeneration therapies increasingly focus on allogeneic cells, specifically mesenchymal stem cells (MSCs), as potent, banked, and available cell sources that express chondrogenic lineage commitment capabilities. Innovative tissue engineering approaches employing allogeneic MSCs aim to develop three-dimensional (3D), chondrogenically differentiated constructs for direct and immediate replacement of hyaline cartilage, improve local site tissue integration, and optimize treatment outcomes. Among emerging tissue engineering technologies, advancements in cell sheet tissue engineering offer promising capabilities for achieving both in vitro hyaline-like differentiation and effective transplantation, based on controlled 3D cellular interactions and retained cellular adhesion molecules. This review focuses on 3D MSC-based tissue engineering approaches for fabricating “ready-to-use” hyaline-like cartilage constructs for future rapid in vivo regenerative cartilage therapies. We highlight current approaches and future directions regarding development of MSC-derived cartilage therapies, emphasizing cell sheet tissue engineering, with specific focus on regulating 3D cellular interactions for controlled chondrogenic differentiation and post-differentiation transplantation capabilities.

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Koh, Rachel H., Jisoo Kim, Seung Hyun L. Kim, and Nathaniel S. Hwang. "RGD-incorporated biomimetic cryogels for hyaline cartilage regeneration." Biomedical Materials 17, no. 2 (February 14, 2022): 024106. http://dx.doi.org/10.1088/1748-605x/ac51b7.

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Abstract Maintaining the integrity of articular cartilage is paramount to joint health and function. Under constant mechanical stress, articular cartilage is prone to injury that often extends to the underlying subchondral bone. In this study, we incorporated arginine–aspartate–glycine (RGD) peptide into chondroitin sulfate-based cryogel for hyaline cartilage regeneration. Known to promote cell adhesion and proliferation, RGD peptide is a double-edged sword for cartilage regeneration. Depending on the peptide availability in the microenvironment, RGD may aid in redifferentiation of dedifferentiated chondrocytes by mimicking physiological cell-matrix interaction or inhibit chondrogenic phenotype via excessive cell spreading. Here, we observed an increase in chondrogenic phenotype with RGD concentration. The group containing the highest RGD concentration (3 mM; RGD group) experienced a 24-fold increase in COL2 expression in the 1st week of in vitro culture and formed native cartilage-resembling ectopic tissue in vivo. No sign of dedifferentiation (COL1) was observed in all groups. Within the concentration range tested (0–3 mM RGD), RGD promotes chondrocyte redifferentiation after monolayer expansion and thus, formation of hyaline cartilage tissue.

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Messaoudi, Océane, Christel Henrionnet, Kevin Bourge, Damien Loeuille, Pierre Gillet, and Astrid Pinzano. "Stem Cells and Extrusion 3D Printing for Hyaline Cartilage Engineering." Cells 10, no. 1 (December 22, 2020): 2. http://dx.doi.org/10.3390/cells10010002.

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Hyaline cartilage is deficient in self-healing properties. The early treatment of focal cartilage lesions is a public health challenge to prevent long-term degradation and the occurrence of osteoarthritis. Cartilage tissue engineering represents a promising alternative to the current insufficient surgical solutions. 3D printing is a thriving technology and offers new possibilities for personalized regenerative medicine. Extrusion-based processes permit the deposition of cell-seeded bioinks, in a layer-by-layer manner, allowing mimicry of the native zonal organization of hyaline cartilage. Mesenchymal stem cells (MSCs) are a promising cell source for cartilage tissue engineering. Originally isolated from bone marrow, they can now be derived from many different cell sources (e.g., synovium, dental pulp, Wharton’s jelly). Their proliferation and differentiation potential are well characterized, and they possess good chondrogenic potential, making them appropriate candidates for cartilage reconstruction. This review summarizes the different sources, origins, and densities of MSCs used in extrusion-based bioprinting (EBB) processes, as alternatives to chondrocytes. The different bioink constituents and their advantages for producing substitutes mimicking healthy hyaline cartilage is also discussed.

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Bielajew, Benjamin J., Jerry C. Hu, and Kyriacos A. Athanasiou. "Methodology to Quantify Collagen Subtypes and Crosslinks: Application in Minipig Cartilages." CARTILAGE 13, no. 2_suppl (November 26, 2021): 1742S—1754S. http://dx.doi.org/10.1177/19476035211060508.

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Introduction This study develops assays to quantify collagen subtypes and crosslinks with liquid chromatography-mass spectrometry (LC-MS) and characterizes the cartilages in the Yucatan minipig. Methods For collagen subtyping, liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was performed on tissues digested in trypsin. For collagen crosslinks, LC-MS analysis was performed on hydrolysates. Samples were also examined histologically and with bottom-up proteomics. Ten cartilages (femoral condyle, femoral head, facet joint, floating rib, true rib, auricular cartilage, annulus fibrosus, 2 meniscus locations, and temporomandibular joint disc) were analyzed. Results The collagen subtyping assay quantified collagen types I and II. The collagen crosslinks assay quantified mature and immature crosslinks. Collagen subtyping revealed that collagen type I predominates in fibrocartilages and collagen type II in hyaline cartilages, as expected. Elastic cartilage and fibrocartilages had more mature collagen crosslink profiles than hyaline cartilages. Bottom-up proteomics revealed a spectrum of ratios between collagen types I and II, and quantified 42 proteins, including 24 collagen alpha-chains and 12 minor collagen types. Discussion The novel assays developed in this work are sensitive, inexpensive, and use a low operator time relative to other collagen analysis methods. Unlike the current collagen assays, these assays quantify collagen subtypes and crosslinks without an antibody-based approach or lengthy chromatography. They apply to any collagenous tissue, with broad applications in tissue characterization and tissue engineering. For example, a novel finding of this work was the presence of a large quantity of collagen type III in the white-white knee meniscus and a spectrum of hyaline and fibrous cartilages.

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Komura, Makoto, Hiroko Komura, Yushi Otani, Yutaka Kanamori, Tadashi Iwanaka, Kazuto Hoshi, Takato Tsuyoshi, and Yasuhiko Tabata. "The junction between hyaline cartilage and engineered cartilage in rabbits." Laryngoscope 123, no. 6 (April 2, 2013): 1547–51. http://dx.doi.org/10.1002/lary.23269.

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Nie, Xiaolei, Yon Jin Chuah, Wenzhen Zhu, Pengfei He, Yvonne Peck, and Dong-An Wang. "Decellularized tissue engineered hyaline cartilage graft for articular cartilage repair." Biomaterials 235 (March 2020): 119821. http://dx.doi.org/10.1016/j.biomaterials.2020.119821.

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Fodor, Pal, Raluca Fodor, Arpad Solyom, Cornel Catoi, Flaviu Tabaran, Radu Lacatus, Cristian Trambitas, Bogdan Cordos, and Tiberiu Bataga. "Autologous Matrix-Induced Chondrogenesis vs Microfracture with PRP for Chondral Lesions of the Knee in a Rabbit Model." Revista de Chimie 69, no. 4 (May 15, 2018): 894–900. http://dx.doi.org/10.37358/rc.18.4.6223.

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Currently, microfracturing is the most commonly used cartilage repair procedure in cartilage defects. Our aim was to study the mechanism of in vivo cartilage repair in case of full-thickness articular cartilage damage of the knee using a three-dimensional matrix implanted without any preseeded cells in the defect. We also investigated whether platelet-rich plasma application after microfracture procedure of the knee is associated with improved outcome compared with traditional microfracture treatment alone in a rabbit model. Histological examination of the chondral defects, revealed the largest amount of new tissue with hyaline-like cartilage features in Hyalofast group. At 12 weeks from implantation of the Hyalofast scaffold demonstrated complete filling of the defect with hyaline cartilage in admixture with the scaffold and bone metaplasia in the deepest areas. In the PRP group, complete filling of the defect with an admixture of fibrous and hyaline-like cartilage tissue appeared with a discreet tendency of endochondral ossification. We confirmed the superiority of the autologous matrix-induced chondrogenesis compared to microfracture and PRP or microfracture alone in case of full-thickness articular cartilage damage of the knee.

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Krause, Fabian, Helen Anwander, and Birgit Schaefer. "Histological Findings Upon Patients Undergoing Revision Surgery after AMIC of the Talus." Foot & Ankle Orthopaedics 7, no. 1 (January 2022): 2473011421S0028. http://dx.doi.org/10.1177/2473011421s00288.

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Category: Ankle Arthritis Introduction/Purpose: Failure after AMIC (autologous matrix-induced chondroplasty) of the talus is relatively rare and ranges between 2-6%. The purpose of the study was to analyze the histologic quality of the repair cartilage and potentially identify the mode of failure in 3 patients that underwent revision surgery after primary AMIC procedures. Methods: Out of 48 patients treated with AMIC for OLT between 2012 and 2018 at our institution, three patients (6.2%, average age 27.3 years) required revision surgery for various reasons. During revision, repair cartilage was evaluated about integration with the surrounding healthy cartilage and firmness, and specimen were taken for histologic assessment. Results: Only one patient that had, next to revision AMIC, a lateral ligament reconstruction for a secondary supination sprain, improved clinically after revision surgery (patient no.3). Despite repair SMOT (no.1) or deltoid ligament repair (no.1), the two other patients did not benefit from revision. Supposed reasons for ongoing symptoms were progressive posttraumatic arthritis (no.1 and 2) and neuropathic pain (no.2). Three types of soft connective tissues could be identified: (i) non-vascularized fibrocartilaginous tissue (patient no. 1-3), (ii) fibrous tissue (patient no. 1 and 2), and (iii) hyaline like cartilage (patient no. 3). Conclusion: Overall, data support the theory that patients with hyaline or hyaline-like repair cartilage tend to better clinical outcome over time. However, the growth of hyaline or hyaline-like repair cartilage appears to require ligamentous stability, neutral or even offloading hindfoot alignment, and absence of arthritis.

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Petrov, A. V., N. V. Matveeva, and A. A. Petrov. "Assessment of clinical significance of ultrasonographic detection ofhyperechogenic deposits in hyaline cartilage in patients with knee osteoarthritis." Rheumatology Science and Practice 57, no. 1 (March 20, 2019): 33–37. http://dx.doi.org/10.14412/1995-4484-2019-33-37.

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The aimof the study was to assess the relationship between the detection of hyperechogenic deposits (HD) in the hyaline cartilage of the knee joints (KJ) at ultrasonography in patients with osteoarthritis (OA), clinical manifestations and structural changes according to KJ ultrasonography and radiography.Material and methods.A prospective analysis of clinical, radiological and ultrasonographic data of 114 patients with knee OA was conducted. The patients were divided into two groups: 32 patients with HD detected in at least one of the KJ, and 82 patients without HD; 32 patients of the 1st group and 34 patients of the 2nd group were observed for 2 years. A comparative assessment of initial clinical manifestations (WOMAC index), x-ray data and ultrasonographic parameters of the subchondral bone, hyaline cartilage and the degree of synovial inflammation in the groups of patients at the beginning of the study and after 2 years was carried out. The exclusion criteria were other joint diseases; paroxysmal course of the inflammatory process in KJ; trauma and history of operations on KJ; ESR >20 mm/h, uric acid level >360 μmol/l and C-reactive protein >5 mg/l.Results and discussion.HD in hyaline cartilage was found in 28.1% of patients with knee OA. After 2 years HD remained in all patients having them at inclusion, and in 5.6% of the patients they were found for the first time. In 13 patients with HD in hyaline cartilage, synovial fluid was studied and in all cases calcium pyrophosphate crystals were identified by phase-contrast microscopy. The presence of HD in the hyaline cartilage of patients with knee OA was accompanied by more pronounced ultrasonographic signs of synovitis and was associated with a higher rate of osteophytes growth in the absence of the changes of the hyaline cartilage thickness according to the ultrasonography and the width of the joint space according to x-ray.Conclusion.The presence of HD in the hyaline cartilage of patients with knee OA according to ultrasound examination may be associated with the deposition of calcium pyrophosphate crystals and is associated with persistent synovitis and accelerated growth of osteophytes.

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Gullberg, S., V. Simaiová, K. Holovská, L. Luptaková, F. Koľvek, M. Varga, and E. Petrovová. "Histological Scoring Systems in the Cartilage Repair of Sheep." Folia Veterinaria 63, no. 4 (December 1, 2019): 15–26. http://dx.doi.org/10.2478/fv-2019-0033.

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Abstract Researchers around the world use histological analysis to provide the most detailed morphological information of articular cartilage repair and it predominantly relies on the use of histological scoring systems which are important tools for valid evaluations. Due to hyaline cartilage complex structure and avascular nature, damaged cartilage does not heal spontaneously and it is still a challenge to regenerate and restore its tissue function. The aim of this study was to investigate the quality of regenerated cartilage by using three different histological scoring systems; O’Driscoll, Pineda and Wakitani which are all classic scores described for such animal studies. We used an in vivo ovine model in which a full thickness chondral defect was created and then implanted with the biomaterial (polyhydroxybutyrate/chitosan; PHB/ CHIT). The results of this histological analysis demonstrated that the cartilage repaired tissues received scores indicating that the majority of the regenerated tissue resembled hyaline-like cartilage. After six months of repair the regenerated cartilage showed characteristics like good surface continuity, uniformed stained extracellular matrix, clearly visible zones and cellular proliferation. In conclusion, this study may be used to investigate and improve the regenerative capacity of hyaline cartilage in preclinical models and it also sheds further light on both the evaluation and methods used for the regeneration of damaged cartilage.

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Chailakhyan, R. K., A. B. Shekhter, V. I. Tel’pukhov, S. V. Ivannikov, Yu V. Gerasimov, N. N. Vorobieva, I. L. Moskvina, and V. N. Bagratashvili. "Repair of Partial Thickness Articular Hyaline Cartilage Injuries with Multipotent Mesenchymal Stromal Bone Marrow Cells Transplantation in Rabbits." N.N. Priorov Journal of Traumatology and Orthopedics 22, no. 1 (March 15, 2015): 23–27. http://dx.doi.org/10.17816/vto201522123-27.

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Possibility of hyaline cartilage integrity restoration using multipotent mesenchymal stromal cells (MMSC) was studied on the rabbit model of partial thickness articular hyaline cartilage defect without subchondral plate damage. Size of defect made up 0.5 cm in diameter and 1.5 mm deep. Autologous bone marrow was harvested from the resected upper flaring portion of the ilium, single cell suspension was prepared and cultured in matrasses. Grown MMSC were centrifuged and the sediment was transferred into the cartilage defect. The cells were covered with either vicryl or gelatin sponge, or vicryl mesh. Histologic examination was performed in 4 months. It was shown that the most active regeneration of hyaline cartilage tissue, that substituted the largest part of a defect, was noted when MMSC were covered with vicryl mesh. One of the advantages of vicryl mesh use was that it neither protruded above the cartilaginous plate nor compressed the cells, and slowly resolved.

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Chailakhyan, R. K., A. B. Shekhter, V. I. Tel’Pukhov, S. V. Ivannikov, Yu V. Gerasimov, N. N. Vorobieva, I. L. Moskvina, and V. N. Bagratashvili. "Repair of Partial Thickness Articular Hyaline Cartilage Injuries with Multipotent Mesenchymal Stromal Bone Marrow Cells Transplantation in Rabbits." Vestnik travmatologii i ortopedii imeni N.N. Priorova, no. 1 (March 30, 2015): 23–27. http://dx.doi.org/10.32414/0869-8678-2015-1-23-27.

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Possibility of hyaline cartilage integrity restoration using multipotent mesenchymal stromal cells (MMSC) was studied on the rabbit model of partial thickness articular hyaline cartilage defect without subchondral plate damage. Size of defect made up 0.5 cm in diameter and 1.5 mm deep. Autologous bone marrow was harvested from the resected upper flaring portion of the ilium, single cell suspension was prepared and cultured in matrasses. Grown MMSC were centrifuged and the sediment was transferred into the cartilage defect. The cells were covered with either vicryl or gelatin sponge, or vicryl mesh. Histologic examination was performed in 4 months. It was shown that the most active regeneration of hyaline cartilage tissue, that substituted the largest part of a defect, was noted when MMSC were covered with vicryl mesh. One of the advantages of vicryl mesh use was that it neither protruded above the cartilaginous plate nor compressed the cells, and slowly resolved.

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Hoffman, James K., Sandra Geraghty, and Nicole M. Protzman. "Articular Cartilage Repair Using Marrow Stimulation Augmented with a Viable Chondral Allograft: 9-Month Postoperative Histological Evaluation." Case Reports in Orthopedics 2015 (2015): 1–10. http://dx.doi.org/10.1155/2015/617365.

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Marrow stimulation is frequently employed to treat focal chondral defects of the knee. However, marrow stimulation typically results in fibrocartilage repair tissue rather than healthy hyaline cartilage, which, over time, predisposes the repair to failure. Recently, a cryopreserved viable chondral allograft was developed to augment marrow stimulation. The chondral allograft is comprised of native viable chondrocytes, chondrogenic growth factors, and extracellular matrix proteins within the superficial, transitional, and radial zones of hyaline cartilage. Therefore, host mesenchymal stem cells that infiltrate the graft from the underlying bone marrow following marrow stimulation are provided with the optimal microenvironment to undergo chondrogenesis. The present report describes treatment of a trochlear defect with marrow stimulation augmented with this novel chondral allograft, along with nine month postoperative histological results. At nine months, the patient demonstrated complete resolution of pain and improvement in function, and the repair tissue consisted of 85% hyaline cartilage. For comparison, a biopsy obtained from a patient 8.2 months after treatment with marrow stimulation alone contained only 5% hyaline cartilage. These outcomes suggest that augmenting marrow stimulation with the viable chondral allograft can eliminate pain and improve outcomes, compared with marrow stimulation alone.

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Malinin, George I., and Theodore I. Malinin. "Microscopic and Histochemical Manifestationsof Hyaline Cartilage Dynamics." Progress in Histochemistry and Cytochemistry 34, no. 3 (January 1999): 163–239. http://dx.doi.org/10.1016/s0079-6336(99)80006-0.

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Goodwin, Douglas W., Haoqin Zhu, and Jeff F. Dunn. "In Vitro MR Imaging of Hyaline Cartilage." American Journal of Roentgenology 174, no. 2 (February 2000): 405–9. http://dx.doi.org/10.2214/ajr.174.2.1740405.

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Ružičková, Mária, Eva Surmíková, and Miroslav Brozman. "Human hyaline cartilage — biochemical and immunochemical study." Acta Histochemica 82, no. 2 (January 1987): 185–91. http://dx.doi.org/10.1016/s0065-1281(87)80026-0.

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Tibesku, C. O., T. Szuwart, T. O. Kleffner, P. M. Schlegel, U. R. Jahn, H. Van Aken, and S. Fuchs. "Hyaline cartilage degenerates after autologous osteochondral transplantation." Journal of Orthopaedic Research 22, no. 6 (November 2004): 1210–14. http://dx.doi.org/10.1016/j.orthres.2004.03.020.

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SCULCO, THOMAS P., OHENEBA BOACHIE-ADJEI, CHARLOTTE CUNNINGHAM-RUNDLES, and SCOTT E. POWELL. "Recurrent Knee Pyarthrosis with Intact Hyaline Cartilage." Clinical Orthopaedics and Related Research &NA;, no. 212 (November 1986): 275???280. http://dx.doi.org/10.1097/00003086-198611000-00030.

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Cova, Maria, and Renato Toffanin. "MR microscopy of hyaline cartilage: current status." European Radiology 12, no. 4 (October 11, 2001): 814–23. http://dx.doi.org/10.1007/s003300101128.

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Guillán-Fresco, María, Eloi Franco-Trepat, Ana Alonso-Pérez, Alberto Jorge-Mora, Miriam López-Fagúndez, Andrés Pazos-Pérez, Oreste Gualillo, and Rodolfo Gómez. "Caffeine, a Risk Factor for Osteoarthritis and Longitudinal Bone Growth Inhibition." Journal of Clinical Medicine 9, no. 4 (April 18, 2020): 1163. http://dx.doi.org/10.3390/jcm9041163.

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Osteoarthritis (OA), the most common chronic rheumatic disease, is mainly characterized by a progressive degradation of the hyaline articular cartilage, which is essential for correct joint function, lubrication, and resistance. Articular cartilage disturbances lead to joint failure, pain, and disability. Hyaline cartilage is also present in the growth plate and plays a key role in longitudinal bone growth. Alterations of this cartilage by diverse pathologies have been related to longitudinal bone growth inhibition (LBGI), which leads to growth retardation. Diet can play a crucial role in processes involved in the OA and LBGI’s onset and evolution. Specifically, there is ample evidence pointing to the negative impacts of caffeine consumption on hyaline cartilage. However, its effects on these tissues have not been reviewed. Accordingly, in this review, we summarize all current knowledge in the PubMed database about caffeine catabolic effects on articular and growth plate cartilage. Specifically, we focus on the correlation between OA and LBGI with caffeine prenatal or direct exposure. Overall, there is ample evidence indicating that caffeine intake negatively affects the physiology of both articular and growth plate cartilage, increasing consumers predisposition to suffer OA and LBGI. As a result, caffeine consumption should be avoided for these pathologies.

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Mizuno, M., T. Takebe, S. Kobayashi, S. Kimura, M. Masutani, S. Lee, Y. H. Jo, J. I. Lee, and H. Taniguchi. "Elastic Cartilage Reconstruction by Transplantation of Cultured Hyaline Cartilage–Derived Chondrocytes." Transplantation Proceedings 46, no. 4 (May 2014): 1217–21. http://dx.doi.org/10.1016/j.transproceed.2013.12.006.

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Bagher, Zohreh, Negin Asgari, Parisa Bozorgmehr, Seyed Kamran Kamrava, Rafieh Alizadeh, and Alexander Seifalian. "Will Tissue-Engineering Strategies Bring New Hope for the Reconstruction of Nasal Septal Cartilage?" Current Stem Cell Research & Therapy 15, no. 2 (March 26, 2020): 144–54. http://dx.doi.org/10.2174/1574888x14666191212160757.

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The nasal septal cartilage plays an important role in the growth of midface and as a vertical strut preventing the collapse of the nasal bones. The repair of nasal cartilage defects remains a major challenge in reconstructive surgery. The tissue engineering strategy in the development of tissue has opened a new perspective to generate functional tissue for transplantation. Given the poor regenerative properties of cartilage and a limited amount of autologous cartilage availability, intense interest has evoked for tissue engineering approaches for cartilage development to provide better outcomes for patients who require nasal septal reconstruction. Despite numerous attempts to substitute the shapely hyaline cartilage in the nasal cartilages, many significant challenges remained unanswered. The aim of this research was to carry out a critical review of the literature on research work carried out on the development of septal cartilage using a tissue engineering approach, concerning different cell sources, scaffolds and growth factors, as well as its clinical pathway and trials have already been carried out.

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Zhang, Zhongwen, Qibin Ye, Zaocheng Yang, Minxia Yin, Jianpeng Bai, Shike Hou, Chunhua Gao, et al. "MATRIX-INDUCED AUTOLOGOUS CHONDROCYTE IMPLANTATION FOR TREATMENT OF CHONDRAL DEFECTS OF KNEE: A PRELIMINARY REPORT." Journal of Musculoskeletal Research 10, no. 02 (June 2006): 95–101. http://dx.doi.org/10.1142/s0218957706001765.

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Objective: To present clinical experience on matrix-induced autologous chondrocyte implantation (MACI), we hereby reported treatment with MACI for 3 patients suffering from chondral lesion of the knee, each of them has been followed for a minimum of 10 months. Methods: Ages of 3 patients were 25, 15 and 32 years old respectively. And the cartilage defect size ranged from 6cm2-10.5cm2). IKDC2000 score was used for knee functional evaluation. Magnetic resonance imaging (MRI) and arthroscopy were performed preoperatively and postoperatively. A biopsy of the regenerated cartilage from one patient was histologically evaluated 15 months after MACI. Results: In the postoperative period, no associated complications were observed. Each patient showed improvements both in clinical and functional status after surgery. MRI and arthroscopy showed the presence of hyaline-like cartilage at the site of implantation. The cartilage biopsy on the first patient showed a high ratio of hyaline-like cartilage tissue to fibrocartilage tissue which was 2 to 1. Conclusion: The clinical outcome and histological evaluation suggest that MACI is able to relieve pain and restore the function of the knee, also is capable of regenerating hyaline cartilage. In conclusion, MACI appears a promising method for the treatment of chondral defects of the knee.

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Vinokurov, Vyacheslav Alexandrovich, and Igor Alekseevich Norkin. "Surgical correction of joint deformities and hyaline cartilage regeneration." Pediatric Traumatology, Orthopaedics and Reconstructive Surgery 3, no. 4 (December 15, 2015): 37–43. http://dx.doi.org/10.17816/ptors3437-43.

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Aim. To determine a method of extra-articular osteochondral fragment formation for the improvement of surgical correction results of joint deformities and optimization of regenerative conditions for hyaline cartilage. Materials and Methods. The method of formation of an articular osteochondral fragment without penetration into the joint cavity was devised experimentally. More than 30 patients with joint deformities underwent the surgery. Results. During the experiments, we postulated that there may potentially be a complete recovery of joint defects because of hyaline cartilage regeneration. By destructing the osteochondral fragment and reforming it extra-articularally, joint defects were recovered in all patients. The results were evaluated as excellent and good in majority of the patients. Conclusion. These findings indicate a novel method in which the complete recovery of joint defects due to dysplastic genesis or osteochondral defects as a result of injuries can be obtained. The devised method can be used in future experiments for objectification and regenerative potential of hyaline cartilage (e.g., rate and volume of the reformed joints that regenerate, detection of cartilage elements, and the regeneration process).

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Guo, Weimin, Xifu Zheng, Weiguo Zhang, Mingxue Chen, Zhenyong Wang, Chunxiang Hao, Jingxiang Huang, et al. "Mesenchymal Stem Cells in Oriented PLGA/ACECM Composite Scaffolds Enhance Structure-Specific Regeneration of Hyaline Cartilage in a Rabbit Model." Stem Cells International 2018 (2018): 1–12. http://dx.doi.org/10.1155/2018/6542198.

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Articular cartilage lacks a blood supply and nerves. Hence, articular cartilage regeneration remains a major challenge in orthopedics. Decellularized extracellular matrix- (ECM-) based strategies have recently received particular attention. The structure of native cartilage exhibits complex zonal heterogeneity. Specifically, the development of a tissue-engineered scaffold mimicking the aligned structure of native cartilage would be of great utility in terms of cartilage regeneration. Previously, we fabricated oriented PLGA/ACECM (natural, nanofibrous, articular cartilage ECM) composite scaffolds. In vitro, we found that the scaffolds not only guided seeded cells to proliferate in an aligned manner but also exhibited high biomechanical strength. To detect whether oriented cartilage regeneration was possible in vivo, we used mesenchymal stem cell (MSC)/scaffold constructs to repair cartilage defects. The results showed that cartilage defects could be completely regenerated. Histologically, these became filled with hyaline cartilage and subchondral bone. Moreover, the aligned structure of cartilage was regenerated and was similar to that of native tissue. In conclusion, the MSC/scaffold constructs enhanced the structure-specific regeneration of hyaline cartilage in a rabbit model and may be a promising treatment strategy for the repair of human cartilage defects.

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Milroy, C. M. "Ossification of the epiglottis." Journal of Laryngology & Otology 106, no. 2 (February 1992): 180–82. http://dx.doi.org/10.1017/s0022215100119036.

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AbstractThe epiglottis is formed of elastic cartilage. Unlike the hyaline cartilage which forms the thyroid cartilage, cricoid and arytenoids, the elastic cartilage of the epiglottis does not undergo ossification with age. A case of ossification of the epiglottis is presented and heterotopic ossification in the head and neck is discussed.

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Vaca-González, Juan J., Johana M. Guevara, Miguel A. Moncayo, Hector Castro-Abril, Yoshie Hata, and Diego A. Garzón-Alvarado. "Biophysical Stimuli: A Review of Electrical and Mechanical Stimulation in Hyaline Cartilage." CARTILAGE 10, no. 2 (September 21, 2017): 157–72. http://dx.doi.org/10.1177/1947603517730637.

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Objective Hyaline cartilage degenerative pathologies induce morphologic and biomechanical changes resulting in cartilage tissue damage. In pursuit of therapeutic options, electrical and mechanical stimulation have been proposed for improving tissue engineering approaches for cartilage repair. The purpose of this review was to highlight the effect of electrical stimulation and mechanical stimuli in chondrocyte behavior. Design Different information sources and the MEDLINE database were systematically revised to summarize the different contributions for the past 40 years. Results It has been shown that electric stimulation may increase cell proliferation and stimulate the synthesis of molecules associated with the extracellular matrix of the articular cartilage, such as collagen type II, aggrecan and glycosaminoglycans, while mechanical loads trigger anabolic and catabolic responses in chondrocytes. Conclusion The biophysical stimuli can increase cell proliferation and stimulate molecules associated with hyaline cartilage extracellular matrix maintenance.

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Dare, E. V., S. G. Vascotto, D. J. Carlsson, M. T. Hincke, and M. Griffith. "Differentiation of a Fibrin Gel Encapsulated Chondrogenic Cell Line." International Journal of Artificial Organs 30, no. 7 (July 2007): 619–27. http://dx.doi.org/10.1177/039139880703000710.

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Hyaline cartilage has very limited regenerative capacity following damage. Therefore engineered tissue substitutes have been the focus of much research. Our objective was to develop a fibrin-based scaffold as a cell delivery vehicle and template for hyaline cartilage regeneration, and compare its cellular properties against monolayer and pellet culture for chondrogenic cells. The chondrogenic precursor cell line, RCJ 3.1C5.18 (C5.18), was chosen as a test system for evaluating the effect of various culture conditions, including cell encapsulation, on articular chondrogenic cell differentiation. The C5.18 cells in monolayer showed elevated expression of collagen II, an articular chondrogenic marker, but also markers for fibrocartilage differentiation (collagen I and versican) when cultured with chondrogenic medium as compared to basic maintenance medium. Pellets of C5.18 cells cultured in chondrogenic medium were histologically more organized in structure than pellets cultured in control maintenance medium. The chondrogenic medium cultured pellets also secreted an extracellular matrix that was comprised of type II with very little type I collagen, indicating a trend towards a more hyaline-like cartilage. Moreover, when cultured in chondrogenic medium, fibrin-encapsulated C5.18 cells elaborated an extracellular matrix containing type II collagen, as well as aggrecan, which are both components of hyaline cartilage. This indicated a more articular-like chondrogenic differentiation for fibrin encapsulated C5.18 cells. The results of these experiments provide evidence that the C5.18 cell line can be used as a tool to evaluate potential scaffolds for articular cartilage tissue engineering. (Int J Artif Organs 2007; 30: 619–27)

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Jimenez, S. A., R. Yankowski, and A. M. Reginato. "Quantitative analysis of type X-collagen biosynthesis by embryonic-chick sternal cartilage." Biochemical Journal 233, no. 2 (January 15, 1986): 357–67. http://dx.doi.org/10.1042/bj2330357.

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We have performed a quantitative analysis of the various collagens biosynthesized by organ cultures of whole embryonic-chick sternum and its separate anatomical regions corresponding to the zones of permanent hyaline and presumptive-calcification cartilages. Our studies demonstrated that embryonic-chick sternum devotes a large portion of its biosynthetic commitment towards production of Type X collagen, which represented approx. 18% of the total newly synthesized collagen. Comparison of the collagens biosynthesized by the permanent hyaline cartilage and by the cartilage from the presumptive-calcification zone demonstrated that Type X-collagen production was strictly confined to the presumptive-calcification region. Sequential extraction of the newly synthesized Type X collagen demonstrated the existence of two separate populations. One population (approx. 20%) was composed of easily extractable molecules that were solubilized with 1.0 m-NaCl/50 mM-Tris/HCI buffer, pH 7.4. The second population was composed of molecules that were not extractable even after repeated pepsin digestion, but became completely solubilized after treatment with 20 mM-dithiothreitol/0.15 M-NaCl buffer at neutral pH. These results suggest that most of the Type X collagen normally exists in the tissue as part of a pepsin-resistant molecular aggregate that may be stabilized by disulphide bonds. Quantitative analysis of the proportion of Type X collagen relative to the other collagens synthesized in the cultures indicated that this collagen was a major biosynthetic product of the presumptive-calcification cartilage, since it represented about 35% of the total collagen synthesized by this tissue. In contrast, the permanent hyaline cartilage did not display any detectable synthesis of Type X collagen. When compared on a per-cell basis, the chondrocytes from the presumptive-calcification zone synthesized approx. 33% more Type X collagen than the amount of Type II collagen synthesized by the chondrocytes from the permanent-hyaline-cartilage zone. Subsequently, it was demonstrated that Type X collagen is a structural component of chick sternum matrix, since quantitative amounts could be extracted from the region of presumptive calcification of 17-day-old chick-embryo sterna and from the calcified portion of adult-chick sterna. The strict topographic distribution in the expression of Type X collagen biosynthesis to the zone of presumptive calcification suggests that this collagen may play an important role in initiation or progression of tissue calcification.

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Cugat, Ramón, Eduard Alentorn-Geli, Gilbert Steinbacher, Pedro Álvarez-Díaz, Xavier Cuscó, Roberto Seijas, David Barastegui, Jordi Navarro, Patricia Laiz, and Montserrat García-Balletbó. "Treatment of Knee Osteochondral Lesions Using a Novel Clot of Autologous Plasma Rich in Growth Factors Mixed with Healthy Hyaline Cartilage Chips and Intra-Articular Injection of PRGF." Case Reports in Orthopedics 2017 (2017): 1–6. http://dx.doi.org/10.1155/2017/8284548.

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Knee cartilage or osteochondral lesions are common and challenging injuries. To date, most symptomatic lesions warrant surgical treatment. We present two cases of patients with knee osteochondral defects treated with a one-step surgical procedure consisting of an autologous-based matrix composed of healthy hyaline cartilage chips, mixed plasma poor-rich in platelets clot, and plasma rich in growth factors (PRGF). Both patients returned to playing soccer at the preinjury activity level and demonstrated excellent defect filling in both magnetic resonance imaging and second-look arthroscopy (in one of them). The use of a clot of autologous plasma poor in platelets with healthy hyaline cartilage chips and intra-articular injection of plasma rich in platelets is an effective, easy, and cheap option to treat knee cartilage injuries in young and athletic patients.

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Palissier, F., I. Raymond-Letron, A. Autefage, and S. Palierne. "A case of bilateral patellar osteochondrosis and fracture in a cat." Veterinary and Comparative Orthopaedics and Traumatology 23, no. 02 (2010): 128–33. http://dx.doi.org/10.3415/vcot-09-06-0066.

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SummaryFracture of the patella associated with bilateral osteochondrosis of the superior pole of the patella in a 14-week-old cat is reported with histological findings.Patellar osteochondrosis has been described in humans, horses, pigs, and dogs and is char-acterised by incomplete union of the ossification centres related to an abnormal process of endochondral ossification. However this disease has not yet been described in cats. Macroscopically, two main fragments separated by interposed tissue were identified on the left patella. In contrast, no fracture but only a fissuration of the articular cartilage was observed on the right patella. Bilateral partial patellectomy was performed. Histological examination of the excised fragments from the left patella revealed two main areas of trabecular bone separated by a wide irregular band of hyaline cartilage. The microscopic aspect of the right patella was similar to that of the left. Serial sections showed the initial appearance of an area of necrosis in the central band of hyaline cartilage, and that this hyaline cartilage was subsequently replaced by fibrovascular connective tissue.These findings indicate that some patellar fractures may be due to patellar osteochondrosis.

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Cobzac, Vitalie, Liliana Verestiuc, Mariana Jian, and Viorel Nacu. "Chondrocytes isolation from hyaline cartilage by continuous monitoring method." Moldovan Medical Journal 64, no. 6 (December 2021): 13–19. http://dx.doi.org/10.52418/moldovan-med-j.64-6.21.03.

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Background: Articular cartilage has poor regenerative capacities. Numerous cartilage repair techniques are known, including implantation of autologous chondrocytes. Material and methods: From 18 rabbits pieces of cartilage were harvested from femoral condyle. Minced cartilage was treated with 0.25% trypsin-EDTA. In the 1st group (n=9) the cartilage was digested with 0.6% collagenase in 15 ml tubes by shaking in incubator at 37°C, 5%CO2 . In the 2nd group (n=9) digestion was performed in 25cm2 cell culture flasks placed on the lateral side, monitoring the process under a microscope after 120 minutes. The isolated cells were cultured to a 80-90% confluence. The chondrocytes were identified using histochemical staining after culturing for 16 days in overconfluence. Results: Chondrocytes isolation in the 1st group lasted a fixed 360 minutes, in the 2nd group – 140±10 minutes. In the 1stgroup were isolated 9.2x104 ±3.1x104 chondrocytes with a viability of 85.36±16.41%, but in the 2nd group – 1.6x105 ±3.4x104 chondrocytes with a viability of 98.09±3.85%. The mean period of cell culture in the 1st group was 15±2 days, in the 2nd group – 11±3 days. In first passage of the 1st group were obtained – 1.2x106 ±4.3x105 chondrocytes and in the 2nd group – 2.92x106 ±3.6x105 chondrocytes. The secreted extracellular matrix by chondrocytes was stained specifically for cartilaginous tissue. Conclusions: The method used for chondrocytes isolation has a direct impact on the number of isolated cells, their viability, but also upon the culture period and the number of cells obtained during the first passage.

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Dou, Zelong, Daniel Muder, Marta Baroncelli, Ameya Bendre, Alexandra Gkourogianni, Lars Ottosson, Torbjörn Vedung, and Ola Nilsson. "Rat perichondrium transplanted to articular cartilage defects forms articular-like, hyaline cartilage." Bone 151 (October 2021): 116035. http://dx.doi.org/10.1016/j.bone.2021.116035.

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Dou, Zelong, Daniel Muder, Marta Baroncelli, Ameya Bendre, Alexandra Gkourogianni, Lars Ottosson, Torbjörn Vedung, and Ola Nilsson. "Rat perichondrium transplanted to articular cartilage defects forms articular-like, hyaline cartilage." Bone Reports 14 (April 2021): 101026. http://dx.doi.org/10.1016/j.bonr.2021.101026.

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McCredie, A. J., E. Stride, and N. Saffari. "Quasi-static elastography comparison of hyaline cartilage structures." Journal of Physics: Conference Series 195 (November 1, 2009): 012004. http://dx.doi.org/10.1088/1742-6596/195/1/012004.

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Journal articles on the topic 'Hyaline cartilage'

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