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Journal articles on the topic 'Hybridization risk'

Author: Grafiati

Published: 2 June 2025

Last updated: 31 July 2025

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1

Wolf, Diana E., Naoki Takebayashi, and Loren H. Rieseberg. "Predicting the Risk of Extinction through Hybridization." Conservation Biology 15, no. 4 (2001): 1039–53. http://dx.doi.org/10.1046/j.1523-1739.2001.0150041039.x.

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2

Nikiforov, Alexander A. "Specificities of Hybrid Risk Formation in the Russian Public Sphere." Sociology of power 35, no. 1 (2023): 219–41. https://doi.org/10.22394/2074-0492-2023-1-219-241.

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Contemporary research in social science often turns to the problem of the hybridization of politics: analyzing the "gray zone" between authoritarianism and democracy; defining the role of new media, and understanding informal practices in restoring peace in post-conflict societies. The contribution of the present article is based on an investigation of the concept of risk hybridization to explore the reflection on risk in the public sphere for the state, social movements, and citizens. The sociology of U. Beck with the theory of reflexive modernization - as well as the concept of cosmopolitics

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Quilodrán, Claudio S., Mathias Currat, and Juan I. Montoya-Burgos. "Effect of hybridization with genome exclusion on extinction risk." Conservation Biology 32, no. 5 (2018): 1139–49. http://dx.doi.org/10.1111/cobi.13120.

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4

Lozada-Gobilard, Sissi, Hana Pánková, Jinlei Zhu, Bojana Stojanova, and Zuzana Münzbergová. "Potential risk of interspecific hybridization in ex situ collections." Journal for Nature Conservation 58 (December 2020): 125912. http://dx.doi.org/10.1016/j.jnc.2020.125912.

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Barth, Thomas F. E., Axel Benner, Martin Bentz, Hartmut D�hner, Peter M�ller, and Peter Lichter. "Risk of false positive results in comparative genomic hybridization." Genes, Chromosomes and Cancer 28, no. 3 (2000): 353–57. http://dx.doi.org/10.1002/1098-2264(200007)28:3<353::aid-gcc15>3.0.co;2-0.

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Raja S and Nagarajan L. "Hybridization of bio-inspired algorithms with machine learning models for predicting the risk of type 2 diabetes mellitus." Scientific Temper 15, no. 03 (2024): 2734–39. http://dx.doi.org/10.58414/scientifictemper.2024.15.3.42.

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Type 2 diabetes mellitus is a chronic condition that affects millions of people worldwide. Predicting the risk of developing this disease is critical for early intervention and prevention. Bio-inspired algorithms and machine learning models have shown promising results in predicting the risk of type 2 diabetes mellitus. In this paper, we will explore the use of these two approaches and their hybridization to improve the accuracy of risk prediction. The first section will introduce bio-inspired algorithms and their application in predicting the risk of type 2 diabetes mellitus. We will discuss

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Fonseca, Rafael. "Role of Fluorescence in Situ Hybridization in Myeloma Risk Stratification." Clinical Lymphoma and Myeloma 9 (February 2009): S6—S8. http://dx.doi.org/10.3816/clm.2009.s.001.

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8

Sánchez-Guillén, R. A., J. Muñoz, J. Hafernik, M. Tierney, G. Rodriguez-Tapia, and A. Córdoba-Aguilar. "Hybridization rate and climate change: are endangered species at risk?" Journal of Insect Conservation 18, no. 3 (2014): 295–305. http://dx.doi.org/10.1007/s10841-014-9637-5.

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Moerman, Lee C., and Sandra L. van der Laan. "Risky business: Socializing asbestos risk and the hybridization of accounting." Critical Perspectives on Accounting 23, no. 2 (2012): 107–16. http://dx.doi.org/10.1016/j.cpa.2011.10.004.

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10

Peterson, Cornelia, Rupin N. Parikh, Meleha T. Ahmad, et al. "Detection of Human Papillomavirus in Squamous Lesions of the Conjunctiva Using RNA and DNA In-Situ Hybridization." International Journal of Molecular Sciences 23, no. 13 (2022): 7249. http://dx.doi.org/10.3390/ijms23137249.

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In-situ hybridization provides a convenient and reliable method to detect human papillomavirus (HPV) infection in formalin-fixed paraffin-embedded tissue. Cases of conjunctival papillomas, conjunctival intraepithelial neoplasia (CIN), conjunctival carcinoma in situ (cCIS), and invasive squamous cell carcinoma (SCC), in which low-risk (LR) and/or high-risk (HR) HPV types were evaluated by RNA or DNA in-situ hybridization, were retrospectively identified. LR HPV types were frequently detected in conjunctival papillomas (25/30, 83%), including 17/18 (94%) with RNA probes, compared to 8/12 (75%) w

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Couroux, Peter R., Zafar Hussain, Frank Rutledge, et al. "Polymerase Chain Reaction-And Rna Hybridization-Based Method for the Investigation of Deep-Seated Candidiasis." Canadian Journal of Infectious Diseases 8, no. 6 (1997): 329–34. http://dx.doi.org/10.1155/1997/520178.

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OBJECTIVE: To determine the usefulness of a polymerase chain reaction (PCR) and RNA hybridization method for the diagnosis of invasive candidiasis and to compare its sensitivity with blood cultures.DESIGN: Blood cultures and a blood sample for PCR were taken from patients with suspected invasive candidiasis. A 105 base pair conserved segment within the rDNA ofCandidaspecies was amplified. The amplicon was detected by hybridization and gel electrophoresis.SETTING: Intensive care units of two tertiary care hospitals.PATIENTS: One hundred and eighteen patients 16 years of age or older with four m

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Urfus, Tomáš, Michaela Pekařová, Ludmila Rejlová, et al. "Urtica kioviensis, a rare species of stinging nettle threatened by hybridization." Preslia 96, no. 4 (2024): 329–49. https://doi.org/10.23855/preslia.2024.329.

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Hybridization is a widespread and important force in plant evolution. It can either hinder speciation or result in the formation of new species. Repeated hybridization with backcrossing with one or both of two hybridizing species is referred to as introgressive hybridization and leads to the introgression of genetic variation from one taxon to another or to other taxa. Hybridization can have consequences for rare species if they are in contact with a more abundant relative with incomplete genetic barriers, as it can lead to genetic erosion, population decline and even the extinction of species

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13

Nuzzo, Fiorella, Vittorio Tison, Antonella Castagnoli, et al. "Human Papillomavirus (HPV) Cervical Lesions: Results from 300 Italian Women Studied with Dna Hybridization Techniques and Morphology." Tumori Journal 74, no. 6 (1988): 745–49. http://dx.doi.org/10.1177/030089168807400621.

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Human papillomavirus cervical infection was investigated in a series of 300 unselected women by comparing morphological diagnoses (cytology and histology) with results of DNA hybridization techniques (filter in situ hybridization of DNA from exfoliated cervical cells and Southern blot analysis of HPV-DNA in cervical biopsy specimens). The prevalence of HPV cervical infection diagnosed by PAP smears was 11.6 %. Despite disadvantages, filter in situ hybridization was confirmed to be particularly useful for screening purposes to detect HPV in cervical scrapings. In 3 cases it was the only applica

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14

Leo, Sarah S. T., and Virginie Millien. "Microsatellite markers reveal low frequency of natural hybridization between the white-footed mouse (Peromyscus leucopus) and deer mouse (Peromyscus maniculatus) in southern Quebec, Canada." Genome 60, no. 5 (2017): 454–63. http://dx.doi.org/10.1139/gen-2016-0163.

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In some parts of southern Quebec, two closely related rodent species — the white-footed mouse (Peromyscus leucopus) and the deer mouse (Peromyscus maniculatus) — have recently come in contact because of climate-driven changes in the distribution of the former. Both species share similar morphology, ecology, and life history traits, which suggests that natural hybridization may be possible. Hybridization among these two species can have important implications on the ecological roles these rodents play in disease transmission, yet few researchers have attempted to examine this phenomenon and res

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15

Jacksen, Jacksen, Angelia Lailatul Jannah, Indah Saraswaty, Suwati Suwati, and Diki Diki. "Survey of HPV genotypes using multiplex PCR and flow through hybridization in Jakarta, Indonesia." Proceeding International Seminar of Science and Technology 3 (February 29, 2024): 33–41. http://dx.doi.org/10.33830/isst.v3i1.2327.

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This is a study to identify genotypes of Human Papilloma Virus (HPV) among women in Jakarta. The virus increases the risk of cervical cancer, which the second highest incident of cancer in Indonesia. Although there is vaccination program to prevent infection of HPV, it must be based on prior screening of recipients based on HPV genotypes. Each genotype has different potential causing premalignant lesion. Most previous studies are not defining the genotypes of HPV. Therefore, this study is conducted to survey genotypes of HPV using Multiplex PCR and Flow Through Hybridization. The Multiplex PCR

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Loeffler, Handy, Flores Quintana, and Deeds. "Fish Hybridization Leads to Uncertainty Regarding Ciguatera Fish Poisoning Risk; Confirmation of Hybridization and Ciguatoxin Accumulation with Implications for Stakeholders." Journal of Marine Science and Engineering 7, no. 4 (2019): 105. http://dx.doi.org/10.3390/jmse7040105.

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Globally, ciguatera fish poisoning (CFP) avoidance efforts rely primarily on local knowledge of the fish being consumed, its collection location, and association with illnesses. In 2016, several fish that appeared to be hybrids between a local commercially prized species, Ocyurus chrysurus, and a regionally prohibited species Lutjanus apodus (due to CFP concerns), were caught nearshore in United States Virgin Islands waters, leading to confusion regarding the safety of consuming the fish. The hybrid status of the fish was verified as O. chrysurus (male) × L. apodus (female) by comparing two se

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Harbicht, Andrew B., Mohammed Alshamlih, Chris C. Wilson, and Dylan J. Fraser. "Anthropogenic and habitat correlates of hybridization between hatchery and wild brook trout." Canadian Journal of Fisheries and Aquatic Sciences 71, no. 5 (2014): 688–97. http://dx.doi.org/10.1139/cjfas-2013-0460.

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Human activities are breaking down barriers to interpopulation hybridization with results that range from populations that resist introgression to populations at serious risk of genetic extinction, particularly between wild and hatchery fish because of the routine stocking of lakes and streams containing wild populations. We investigated whether specific human actions and (or) habitat characteristics were associated with the extent of hybridization between hatchery and wild brook trout (Salvelinus fontinalis) occupying lakes in Algonquin Park, Ontario, Canada. Hatchery and wild trout tended to

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18

Ellstrand, Norman C. "Current knowledge of gene flow in plants: implications for transgene flow." Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences 358, no. 1434 (2003): 1163–70. http://dx.doi.org/10.1098/rstb.2003.1299.

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Plant evolutionary biologists' view of gene flow and hybridization has undergone a revolution. Twenty–five years ago, both were considered rare and largely inconsequential. Now gene flow and hybridization are known to be idiosyncratic, varying with the specific populations involved. Gene flow typically occurs at evolutionarily significant rates and at significant distances. Spontaneous hybridization occasionally has important applied consequences, such as stimulating the evolution of more aggressive invasives and increasing the extinction risk for rare species. The same problems have occurred

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19

Munshi, Nikhil C., Kenneth C. Anderson, P. Leif Bergsagel, et al. "Consensus recommendations for risk stratification in multiple myeloma: report of the International Myeloma Workshop Consensus Panel 2." Blood 117, no. 18 (2011): 4696–700. http://dx.doi.org/10.1182/blood-2010-10-300970.

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Abstract A panel of members of the 2009 International Myeloma Workshop developed guidelines for risk stratification in multiple myeloma. The purpose of risk stratification is not to decide time of therapy but to prognosticate. There is general consensus that risk stratification is applicable to newly diagnosed patients; however, some genetic abnormalities characteristic of poor outcome at diagnosis may suggest poor outcome if only detected at the time of relapse. Thus, in good-risk patients, it is necessary to evaluate for high-risk features at relapse. Although detection of any cytogenetic ab

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20

Avet-Loiseau, Hervé. "Ultra High-Risk Myeloma." Hematology 2010, no. 1 (2010): 489–93. http://dx.doi.org/10.1182/asheducation-2010.1.489.

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Abstract Ultra high-risk myeloma can be defined as myeloma leading to death within 24 months. Despite tremendous improvements in the past decade (especially because of the availability of novel drugs such as thalidomide, bortezomib, and lenalidomide), these patients still represent 15% to 20% of the patients. Many prognostic factors can help to define these patients, including age, renal insufficiency, poor performance status, comorbities, International Staging System (ISS) stage 3, high proliferation, leukemic presentation, and acquired genetic changes, as defined by interphase fluorescence i

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21

Zaravinos, Apostolos, Ioannis N. Mammas, George Sourvinos, and Demetrios A. Spandidos. "Molecular detection methods of human papillomavirus (HPV)." International Journal of Biological Markers 24, no. 4 (2009): 215–22. http://dx.doi.org/10.1177/172460080902400401.

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Human papillomavirus (HPV) testing can identify women at risk of cervical cancer. Currently, molecular detection methods are the gold standard for identification of HPV. The three categories of molecular assays that are available are based on the detection of HPV DNA and include (1) non-amplified hybridization assays, such as Southern transfer hybridization (STH), dot blot hybridization (DB) and in situ hybridization (ISH); (2) signal amplified hybridization assays, such as hybrid capture assays (HC2); (3) target amplification assays, such as polymerase chain reaction (PCR) and in situ PCR. ST

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22

Lapidot-Lifson, Yaron, Roger V. Lebo, Robert R. Flandermeyer, Jae-Hyun Chung, and Mitchell S. Golbus. "Rapid aneuploid diagnosis of high-risk fetuses by fluorescence in situ hybridization." American Journal of Obstetrics and Gynecology 174, no. 3 (1996): 886–90. http://dx.doi.org/10.1016/s0002-9378(96)70319-8.

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23

Brukner, Ivan, Razan El-Ramahi, Jacob Sawicki, Izabella Gorska-Flipot, Maja Krajinovic, and Damian Labuda. "Hybridization assay performed at ambient temperature for typing high-risk human papillomaviruses." Journal of Clinical Virology 39, no. 2 (2007): 113–18. http://dx.doi.org/10.1016/j.jcv.2007.03.013.

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24

Hittelman, Walter N., Hyo J. Kim, Jin S. Lee, et al. "Detection of chromosome instability of tissue fields at risk: In situ hybridization." Journal of Cellular Biochemistry 63, S25 (1996): 57–62. http://dx.doi.org/10.1002/(sici)1097-4644(1996)25+<57::aid-jcb8>3.0.co;2-1.

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25

Moreno, Elizabeth Castro, Maria Norma Melo, Odair Genaro, et al. "Risk factors for Leishmania chagasi infection in an urban area of Minas Gerais State." Revista da Sociedade Brasileira de Medicina Tropical 38, no. 6 (2005): 456–63. http://dx.doi.org/10.1590/s0037-86822005000600002.

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In order to understand the determinants of human infection by Leishmania chagasi in an urban area, a cross-sectional population based study was conducted using molecular and serologic methods to identify infection. Participants were interviewed using a pre-coded questionnaire. Two criteria were tested to identify risk factors: Model 1- including all participants positive in hybridization by Leishmania donovani complex probe; Model 2- including all participants positive for hybridization and at least one serologic test. In Model 1, the variables associated with infection were: ownership of bird

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Luxton, Jared J., Miles J. McKenna, Aidan M. Lewis, et al. "Telomere Length Dynamics and Chromosomal Instability for Predicting Individual Radiosensitivity and Risk via Machine Learning." Journal of Personalized Medicine 11, no. 3 (2021): 188. http://dx.doi.org/10.3390/jpm11030188.

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The ability to predict a cancer patient’s response to radiotherapy and risk of developing adverse late health effects would greatly improve personalized treatment regimens and individual outcomes. Telomeres represent a compelling biomarker of individual radiosensitivity and risk, as exposure can result in dysfunctional telomere pathologies that coincidentally overlap with many radiation-induced late effects, ranging from degenerative conditions like fibrosis and cardiovascular disease to proliferative pathologies like cancer. Here, telomere length was longitudinally assessed in a cohort of fif

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Lee, Jonathan J., and Christine G. Lian. "Molecular Testing for Cutaneous Melanoma: An Update and Review." Archives of Pathology & Laboratory Medicine 143, no. 7 (2018): 811–20. http://dx.doi.org/10.5858/arpa.2018-0038-ra.

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Context.— The steady rise in the incidence of cutaneous malignant melanoma and its inherently difficult-to-interpret histopathology continues to fuel an increasing demand for diagnostically and prognostically insightful adjunctive molecular tests among both clinicians and dermatopathologists. A number of DNA, RNA, and epigenetically based assays have now been developed and are at various stages of experimental and/or clinical use. Objective.— To examine the evidence for the utility and limitations of these leading candidates for the diagnosis and risk stratification of melanoma and related mel

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Brankley, Shannon, Yvonne Romero, Michael B. Campion, Debra M. Geno, and Kevin C. Halling. "Sa1979 Fluorescence in Situ Hybridization Analysis on a Low-Risk Barrett's Esophagus Population." Gastroenterology 144, no. 5 (2013): S—350. http://dx.doi.org/10.1016/s0016-5085(13)61288-3.

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Vettenranta, Kim, Yan Aalto, Sakari Wikström, Sakari Knuutila, and Ulla Saarinen-Pihkala. "Comparative genomic hybridization reveals changes in DNA-copy number in poor-risk neuroblastoma." Cancer Genetics and Cytogenetics 125, no. 2 (2001): 125–30. http://dx.doi.org/10.1016/s0165-4608(00)00366-6.

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López-Caamal, Alfredo, Zenón Cano-Santana, Jaime Jiménez-Ramírez, Rolando Ramírez-Rodríguez, and Efraín Tovar-Sánchez. "Is the insular endemic Psidium socorrense (Myrtaceae) at risk of extinction through hybridization?" Plant Systematics and Evolution 300, no. 9 (2014): 1959–72. http://dx.doi.org/10.1007/s00606-014-1025-9.

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Alizi, Sana'a M. H., Faiza A. Mukhlis, and Ban A. Abdul-Majeed. "Detection of Human papillomavirus in surface epithelial ovarian carcinoma using in situ hybridization technique." Journal of the Faculty of Medicine Baghdad 54, no. 1 (2012): 57–62. http://dx.doi.org/10.32007/jfacmedbagdad.541772.

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Background: The role of Human papillomaviruses (HPV) in the etiology of ovarian cancer remains unclear and the results are controversial. Several studies have verified the presence of HPV DNA in both malignant and benign ovarian tumors.Objectives: Determine the percentage of detection of HPV high (16&18) and low risk types (6&11) in surface epithelial ovarian carcinoma compared to benign and control groups.Materials And Methods: Molecular detection and genotyping of HPV DNA were performed in 76 ovarian tissue blocks by using in situ hybridization (ISH) technique for detecting and local

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Ma, Chun-Ho (Charlie), Liejun Li, Shuheng Cai, et al. "Development of an HPV Genotype Detection Platform Based on Aggregation-Induced Emission (AIE) and Flow-Through Hybridization Technologies." Molecules 27, no. 20 (2022): 7036. http://dx.doi.org/10.3390/molecules27207036.

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Genetic mutations can cause life-threatening diseases such as cancers and sickle cell anemia. Gene detection is thus of importance for disease-risk prediction or early diagnosis and treatment. Apart from genetic defects, gene detection techniques can also be applied to gene-related diseases with high risk to human health such as human papillomavirus (HPV) infection. HPV infection has been strongly linked to cervical cancer. To achieve a high-throughput HPV gene detection platform, the flow-through hybridization system appears to be one of the commercialized diagnostic techniques for this purpo

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Park, Sarah, Jeeyun Lee, Young Hyeh Ko, et al. "The impact of Epstein-Barr virus status on clinical outcome in diffuse large B-cell lymphoma." Blood 110, no. 3 (2007): 972–78. http://dx.doi.org/10.1182/blood-2007-01-067769.

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AbstractTo define prognostic impact of Epstein-Barr virus (EBV) infection in diffuse large B-cell lymphoma (DLBCL), we investigated EBV status in patients with DLBCL. In all, 380 slides from paraffin-embedded tissue were available for analysis by EBV-encoded RNA-1 (EBER) in situ hybridization, and 34 cases (9.0%) were identified as EBER-positive. EBER positivity was significantly associated with age greater than 60 years (P = .005), more advanced stage (P < .001), more than one extranodal involvement (P = .009), higher International Prognostic Index (IPI) risk group (P = .015), presence of

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Hose, Dirk, Anja Seckinger, Anna Jauch, et al. "The role of fluorescence in situ hybridization and gene expression profiling in myeloma risk stratification." Srpski arhiv za celokupno lekarstvo 139, suppl. 2 (2011): 84–89. http://dx.doi.org/10.2298/sarh11s2084h.

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Multiple myeloma patients? survival under treatment varies from a few months to more than 15 years. Clinical prognostic factors, especially beta2-microglobulin (B2M) and the international staging system (ISS), allow risk assessment to a certain extent, but do not identify patients at very high risk. As malignant plasma cells are characterized by a variety of chromosomal aberrations and changes in gene expression, a molecular characterization of CD138-purified myeloma cells by interphase fluorescence in situ hybridization (iFISH) and gene expression profiling (GEP) can be used for improved risk

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Davies, Faith E., and Brian A. Walker. "What Is Genomic High-Risk Myeloma?" Hemato 3, no. 2 (2022): 287–97. http://dx.doi.org/10.3390/hemato3020021.

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Although treatment of multiple myeloma has changed dramatically over time, there is still a subpopulation of patients who do not respond to treatments and are labeled as high risk. A combination of serum and genomic markers can be used to identify and stratify these patients according to associations with outcome. The most common method of identifying the genomic markers of high-risk multiple myeloma is using fluorescence in situ hybridization using probes to identify IgH translocations or copy number changes including the t(4;14), t(14;16), t(14;20), gain 1q, and del(17p). However, as researc

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Salomao, Marcela, Brynn Levy, Odelia Nahum, Jinli Chen, Mahesh Mansukhani, and Alain C. Borczuk. "Genomic Alterations in Pulmonary Adenocarcinoma In Situ in an Adolescent Patient." Archives of Pathology & Laboratory Medicine 138, no. 4 (2014): 559–63. http://dx.doi.org/10.5858/arpa.2012-0741-cr.

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Lung cancer is a rare event in the pediatric and adolescent population. To date, only a few case reports and small case series have been published, and little is known about the risk factors associated with this entity in children and adolescents. We describe a case of adenocarcinoma in situ in a 15-year-old adolescent girl with previous surgical treatment for malignant melanoma. We provide a detailed genomic characterization of this neoplasm by comparative genomic hybridization, genome-wide single-nucleotide polymorphism array, and fluorescence in situ hybridization analyses. We identify chro

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CORRÊA, A. A., J. TOSO, J. D. ALBARNAZ, C. M. O. SIMÕES, and C. R. M. BARARDI. "DETECTION OF SALMONELLA TYPHIMURIUM IN OYSTERS BY PCR AND MOLECULAR HYBRIDIZATION." Journal of Food Quality 29, no. 5 (2006): 458–69. http://dx.doi.org/10.1111/j.1745-4557.2006.00086.x.

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Lee, Chang Joon, Andres Salas Montoya, Hoon Moon, Hyunwook Kim, and Chul-Woo Chung. "The Influence of the Hybridization of Steel and Polyolefin Fiber on the Mechanical Properties of Base Concrete Designed for Marine Shotcreting Purposes." Applied Sciences 11, no. 20 (2021): 9456. http://dx.doi.org/10.3390/app11209456.

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The present study investigated the influence of the hybridization of steel and polyolefin fiber on the mechanical performance and chloride ion penetration of base concrete designed for marine shotcreting purposes. The purpose of fiber hybridization is to reduce the risk of corrosion that might occur during service life. Sets of hybrid fiber reinforced base concrete, whose water to binder ratio was 0.338, were prepared. The fiber contents in the base concrete were 0.54 and 1.08 vol%, and the volume proportion of polyolefin fiber in the hybrid fiber varied from 0 to 100%. Although the effect of

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Brankley, S. M., K. C. Halling, S. M. Jenkins, et al. "Fluorescence in situ hybridization identifies high risk Barrett's patients likely to develop esophageal adenocarcinoma." Diseases of the Esophagus 29, no. 6 (2015): 513–19. http://dx.doi.org/10.1111/dote.12372.

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Hinna, Alessandro, Danila Scarozza, and Fabrizio Rotundi. "Implementing Risk Management in the Italian Public Sector: Hybridization between Old and New Practices." International Journal of Public Administration 41, no. 2 (2017): 110–28. http://dx.doi.org/10.1080/01900692.2016.1255959.

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Kim, B., and M. P. Bronner. "Fluorescence in situ hybridization biomarkers of gastric cancer risk in intestinalized Helicobacter pylori pangastritis." Journal of Clinical Oncology 29, no. 4_suppl (2011): 17. http://dx.doi.org/10.1200/jco.2011.29.4_suppl.17.

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17 Background: H. pylori (HP) intestinalized pangastritis predisposes to gastric adenocarcinoma. Biomarkers to identify the less than 10% subset of chronic gastritis patients who will develop gastric cancer are greatly needed to focus surveillance efforts onto those patients most likely to benefit. Investigations in ulcerative colitis and Barrett's esophagus reveal that chromosomal alterations in far distant and nondysplastic mucosa are powerful cancer biomarkers. We hypothesized a similar situation in gastric tumorigenesis. Methods: The full morphologic spectrum of gastric neoplasia was exami

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Homer, Jeanne, Sucheta Bhatt, Bing Huang, and Maya Thangavelu. "Residual risk for cytogenetic abnormalities after prenatal diagnosis by interphase fluorescencein situ hybridization (FISH)." Prenatal Diagnosis 23, no. 7 (2003): 566–71. http://dx.doi.org/10.1002/pd.642.

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Skorodumova, L. O., K. A. Babalyan, R. Sultanov, et al. "GSTP1, APC and RASSF1 gene methylation in prostate cancer samples: comparative analysis of MS-HRM method and Infinium HumanMethylation450 BeadChip beadchiparray diagnostic value." Biomeditsinskaya Khimiya 62, no. 6 (2016): 708–14. http://dx.doi.org/10.18097/pbmc20166206708.

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There is a clear need in molecular markers for prostate cancer (PC) risk stratification. Alteration of DNA methylation is one of processes that occur during ÐÑ progression. Methylation-sensitive PCR with high resolution melting curve analysis (MS-HRM) can be used for gene methylation analysis in routine laboratory practice. This method requires very small amounts of DNA for analysis. Numerous results have been accumulated on DNA methylation in PC samples analyzed by the Infinium HumanMethylation450 BeadChip (HM450). However, the consistency of MS-HRM results with chip hybridization results has

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Sherman, C. D. H., E. Wapstra, T. Uller, and M. Olsson. "Males with high genetic similarity to females sire more offspring in sperm competition in Peron's tree frog Litoria peronii." Proceedings of the Royal Society B: Biological Sciences 275, no. 1637 (2008): 971–78. http://dx.doi.org/10.1098/rspb.2007.1626.

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Recent work has confirmed that genetic compatibility among mates can be an important determinant of siring success in sperm competition experiments and in free-ranging populations. Most of this work points towards mate choice of less related mates. However, there may also be the potential for mate choice for intermediate or even genetically similar mates to prevent outbreeding depression or hybridization with closely related taxa. We studied relatedness effects on post-copulatory gametic choice and/or sperm competition in an external fertilizer, Peron's tree frog ( Litoria peronii ), since ext

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Parmin, N. A., Uda Hashim, Wan Azani Mustafa, S. C. B. Gopinath, Zulida Rejali, and M. N. A. Uda. "In Vitro Nucleic Acid Hybridization for the Identification of High-Risk Human Papillomavirus (HPV) in Cervical Clinical Specimens." Journal of Biomimetics, Biomaterials and Biomedical Engineering 42 (July 2019): 51–58. http://dx.doi.org/10.4028/www.scientific.net/jbbbe.42.51.

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The reliability and specificity of the Pap smear rely on the eyewitness to perceive and an assortment of cell variations in clinically obtained cervical specimens. The improvement in early diagnosis to distinguish Human Papillomavirus (HPV) infection has been an issue. ELISA has been intended to analyze the immune response against HPV, and they can be utilized to distinguish the phase of the infection. The objective of this study to characterize the performance of In Vitro Nucleic Acid Hybridization with a correlation with Liquid-Based Cytology and HPV DNA Genotyping Test to assess its use as

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Savage, Kerry J., Nathalie A. Johnson, Susana Ben-Neriah, et al. "MYC gene rearrangements are associated with a poor prognosis in diffuse large B-cell lymphoma patients treated with R-CHOP chemotherapy." Blood 114, no. 17 (2009): 3533–37. http://dx.doi.org/10.1182/blood-2009-05-220095.

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Abstract Approximately 5% to 10% of diffuse large B-cell lymphomas (DLBCLs) harbor an MYC oncogene rearrangement (MYC+). The prognostic significance of MYC+ DLBCL was determined in an unselected population of patients with newly diagnosed DLBCL treated with rituximab in combination with cyclophosphamide, doxorubicin, vincristine, and prednisone chemotherapy (R-CHOP). Using a Vysis break-apart fluorescence in situ hybridization probe, 12 of 135 (8.8%) cases of MYC+ DLBCL were identified that had no defining high-risk features. MYC+ DLBCL was associated with an inferior 5-year progression-free s

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Press, M. F., L. Bernstein, P. A. Thomas, et al. "HER-2/neu gene amplification characterized by fluorescence in situ hybridization: poor prognosis in node-negative breast carcinomas." Journal of Clinical Oncology 15, no. 8 (1997): 2894–904. http://dx.doi.org/10.1200/jco.1997.15.8.2894.

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PURPOSE The HER-2/neu gene codes for a membrane receptor protein that is homologous, but distinct from the epidermal growth factor receptor. This investigation was performed to validate fluorescence in situ hybridization (FISH) as a sensitive and specific method for assessing HER-2/neu gene amplification in archival tissue and to test whether this alteration is associated with poor prognosis. MATERIALS AND METHODS HER-2/neu gene amplification was determined by FISH in 140 archival breast cancers, previously characterized for gene amplification by Southern hybridization or dot-blot hybridizatio

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Paris, P. L., V. Weinberg, J. Simko, et al. "Preliminary Evaluation of Prostate Cancer Metastatic Risk Biomarkers." International Journal of Biological Markers 20, no. 3 (2005): 141–45. http://dx.doi.org/10.1177/172460080502000301.

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Prostate cancer patients at high risk of metastasis need to be identified as early as possible since metastasis is invariably fatal. Treatment could be tailored to risk. Recent array comparative genomic hybridization (aCGH) studies of primary and metastatic prostate tumors identified 39 BAC clones capable of detecting genomic signatures of metastasis. We termed these loci the genomic evaluators of metastatic CaP (GEMCaP). Risk assessments were made on a set of men who were managed with radical prostatectomy. We compared the utility of GEMCaP loci and the Kattan nomogram, a common risk assessme

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Alimardani, Arezou, Narges Dastmalchi, Hamzeh Rahimi, and Reza Safaralizadeh. "Designing a Sequence-Based Method for Identifying 14 High-Risk Carcinogenic HPV Types in Multiple Infections." Infectious Disorders - Drug Targets 22, no. 3 (2022): 72–88. http://dx.doi.org/10.2174/1871526522666220127115120.

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Background: HPV tests have significant drawbacks in terms of detecting and differentiating types of the virus. PCR techniques provide timely and necessary results for patient care with high quality, sensitivity, and reasonable cost. Methods: The sensitivity of PCR depends on the primers. In this study, a method was designed that exploited PCR with designed primers (ScTd) by changing the annealing temperature (Ta) along with Sanger sequencing for pap smear samples. Sanger sequencing has confirmed that ScTd primers have a relative differentiation power using PCR. The primers caused a relative di

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Korshunov, Andrey, Marc Remke, Wiebke Werft, et al. "Adult and Pediatric Medulloblastomas Are Genetically Distinct and Require Different Algorithms for Molecular Risk Stratification." Journal of Clinical Oncology 28, no. 18 (2010): 3054–60. http://dx.doi.org/10.1200/jco.2009.25.7121.

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Purpose Medulloblastoma (MB) is the most common malignant brain tumor in children, whereas it rarely presents in adults. We aimed to identify genetic aberrations in 146 adult MBs to evaluate age-dependent differences in tumor biology and adapt age-specific risk stratification models. Methods As a screening set, we studied a cohort of 34 adult MBs by using array-based comparative genomic hybridization comparing molecular results with clinical data. DNA copy number aberrations identified as possible prognostic markers were validated in an independent cohort of 112 adult patients with MB by fluor

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