Academic literature on the topic 'Hybridoma's'

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Journal articles on the topic "Hybridoma's"

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Ozaki, S., S. K. Durum, K. Muegge, J. York-Jolley, and J. A. Berzofsky. "Production of T-T hybrids from T cell clones. Direct comparison between cloned T cells and T hybridoma cells derived from them." Journal of Immunology 141, no. 1 (1988): 71–78. http://dx.doi.org/10.4049/jimmunol.141.1.71.

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Abstract It has been assumed, without direct evidence, that T cell hybridomas and non-transformed T cell clones are both good models of normal Ag-specific T cells. To compare directly the difference in activation of cloned normal T cells and T hybridoma cells with the same TCR, cloned T hybridoma cells were obtained by fusing pre-established, myoglobin-specific, Iad-restricted T cell clones (14.5 and 9.27) with BW5147 cells. T cell clones were pre-activated with IL-2 as well as specific Ag before fusion. Cloned T hybridoma A3.4C6 was derived from Lys 140-specific and I-Ed-restricted clone 14.5
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Hawksworth, David, Jeff Moore, and Isaac Larkin. "Diversity and affinity from plasma vs. B cells for monoclonal antibody development." Journal of Immunology 212, no. 1_Supplement (2024): 0234_4543. http://dx.doi.org/10.4049/jimmunol.212.supp.0234.4543.

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Abstract Diagnostic assay development requires a diverse panel of high affinity antibodies. Historically, our lab has used splenic CD19+ B-cells to generate mouse hybridoma cell lines. However, literature suggests that, as a group, terminally differentiated, CD138+ plasma cells secrete the highest affinity antibodies, making these cells an attractive target for hybridoma development. To compare the use of these two lymphocyte populations for generating mouse hybridomas, three fusion experiments were completed whereby cells were separately isolated and fused. Hybridomas were plated in semi-soli
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Rauch, J., H. Massicotte, and H. Tannenbaum. "Hybridoma anti-DNA autoantibodies from patients with rheumatoid arthritis and systemic lupus erythematosus demonstrate similar nucleic acid binding characteristics." Journal of Immunology 134, no. 1 (1985): 180–86. http://dx.doi.org/10.4049/jimmunol.134.1.180.

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Abstract Hybridoma anti-DNA antibodies have been generated from the fusion of the GM 4672 lymphoblastoid line with peripheral blood lymphocytes from four normal subjects, nine patients with rheumatoid arthritis (RA), and 13 patients with systemic lupus erythematosus (SLE). A total of 441 hybridoma clones were obtained, of which 37 secreted anti-DNA autoantibodies. The nucleic acid binding characteristics of the anti-DNA antibodies produced by two hybridomas from normal subjects, nine hybridomas from RA patients, and 18 hybridomas from SLE patients are reported. The hybridoma anti-DNA antibodie
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Kuchroo, V. K., P. R. Billings, C. Levine, C. A. Martin, R. T. Kubo, and M. E. Dorf. "Antigen-specific and antibody-mediated growth inhibition of suppressor T cell hybridomas. Roles of H-2 and the CD3-T cell receptor-alpha/beta complex." Journal of Immunology 145, no. 4 (1990): 1059–65. http://dx.doi.org/10.4049/jimmunol.145.4.1059.

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Abstract Eight different Ts cell hybridomas (including inducer (Ts1) and effector (Ts3) suppressor cells) specific for the 4-hydroxy-3-nitrophenyl acetyl (NP) hapten were tested for their ability to respond to Ag or anti-CD3 antibody in a growth-inhibition assay. Results suggest that the expression of the TCR-CD3 complex on Ts hybridomas is required for the Ag or anti-CD3-mediated growth inhibition. One of the CD3+, Ts hybridomas (CKB-Ts3-9.H3) was tested in detail; this CD4- effector suppressor cell hybridoma showed specific inhibition of growth in the presence of NP or NIP-coupled protein co
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Machida, Keigo, Yasuteru Kondo, Jeffrey Y. Huang, et al. "Hepatitis C Virus (HCV)-Induced Immunoglobulin Hypermutation Reduces the Affinity and Neutralizing Activities of Antibodies against HCV Envelope Protein." Journal of Virology 82, no. 13 (2008): 6711–20. http://dx.doi.org/10.1128/jvi.02582-07.

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ABSTRACT Hepatitis C virus (HCV) often causes persistent infection despite the presence of neutralizing antibodies against the virus in the sera of hepatitis C patients. HCV infects both hepatocytes and B cells through the binding of its envelope glycoprotein E2 to CD81, the putative viral receptor. Previously, we have shown that E2-CD81 interaction induces hypermutation of heavy-chain immunoglobulin (V H ) in B cells. We hypothesize that if HCV infects antibody-producing B cells, the resultant hypermutation of VH may lower the affinity and specificity of the HCV-specific antibodies, enabling
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Retter, M. W., R. A. Eisenberg, P. L. Cohen, and S. H. Clarke. "Sm and DNA binding by dual reactive B cells requires distinct VH, V kappa, and VH CDR3 structures." Journal of Immunology 155, no. 4 (1995): 2248–57. http://dx.doi.org/10.4049/jimmunol.155.4.2248.

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Abstract We have previously demonstrated an overlap of the anti-Sm and anti-DNA responses in MRL/Mp-lpr/lpr mice. The Ab produced by many anti-Sm hybridomas bind DNA and are encoded by Ig V genes used by anti-DNA hybridomas. In addition, some anti-Sm Ab that bind DNA have acquired mutations that improve DNA binding, indicating that DNA is a selecting Ag in the anti-Sm response. To gain insight into the basis for the dual binding ability of these Ab, we coexpressed the H chain from the anti-Sm hybridoma 2-12 with nine different L chains. Hybridoma 2-12 binds Sm but not DNA, yet expresses the sa
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Tiebout, R. F., F. van Boxtel-Oosterhof, E. A. Stricker, and W. P. Zeijlemaker. "A human hybrid hybridoma." Journal of Immunology 139, no. 10 (1987): 3402–5. http://dx.doi.org/10.4049/jimmunol.139.10.3402.

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Abstract Hybrid hybridomas are obtained by fusion of two cells, each producing its own antibody. Several authors have reported the construction of murine hybrid hybridomas with the aim to obtain bispecific monoclonal antibodies. We have investigated, in a model system, the feasibility of constructing a human hybrid hybridoma. We fused two monoclonal cell lines: an ouabain-sensitive and azaserine/hypoxanthine-resistant Epstein-Barr virus-transformed human cell line that produces an IgG1 kappa antibody directed against tetanus toxoid and an azaserine/hypoxanthine-sensitive and ouabain-resistant
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Rath, S., J. Durdik, R. M. Gerstein, E. Selsing, and A. Nisonoff. "Quantitative analysis of idiotypic mimicry and allelic exclusion in mice with a mu Ig transgene." Journal of Immunology 143, no. 6 (1989): 2074–80. http://dx.doi.org/10.4049/jimmunol.143.6.2074.

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Abstract Analysis of C57BL/6 mice (IgM allotype, Igh-6b or mu b) that carry an Ig H chain transgene of a different allotype (mu a) shows that IgM molecules of mixed allotype (mu a mu b) are present among serum antibodies. The finding was extended to hybridomas prepared from nonimmune transgenic mice, many of which also failed to exhibit allelic exclusion. The proportions of mu a and mu b secreted by individual hybridomas varied markedly, and the product of an individual hybridoma was found to be heterogeneous with respect to the allotype content of individual molecules. The ratio of mu a:mu b
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Mathur, A., B. G. Van Ness, and R. G. Lynch. "In vivo and in vitro regulation of IgE production in murine hybridomas." Journal of Immunology 145, no. 11 (1990): 3610–17. http://dx.doi.org/10.4049/jimmunol.145.11.3610.

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Abstract Normal BALB/c mice injected i.p. with the IgE-secreting hybridomas B53 (epsilon, kappa anti-DNP), SE1.3 (epsilon, kappa, anti-arsonate) or A3B1 (epsilon, kappa, anti-TNP) were monitored for serum IgE concentrations and frequencies of splenic T lymphocytes with surface membrane receptors for the Fc portion of IgE (Fc epsilon R+ T lymphocytes). Mice with B53 or SE1.3 hybridomas initially developed high concentrations of IgE and CD8+ Fc epsilon R+ T lymphocytes, followed by a progressive decline in both serum IgE and expression of cytoplasmic epsilon-chains in the hybridoma cells. Serum
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Kawasaki, H., C. A. Martin, T. Uchida, et al. "Functional analysis of cloned macrophage hybridomas. V. Induction of suppressor T cell responses." Journal of Immunology 137, no. 7 (1986): 2145–51. http://dx.doi.org/10.4049/jimmunol.137.7.2145.

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Abstract It has been suggested that macrophage-like accessory cells are involved in suppressor T cell (Ts) induction. To further analyze this issue, we obtained several cloned macrophage hybridoma cell lines by somatic cell fusion of the macrophage tumor P388D1 of DBA/2 (H-2d) origin with splenic adherent cells of CKB mice (H-2k). Several cloned lines displayed the serological and functional characteristics of macrophages. We evaluated the ability of these hybridomas to induce third order or effector Ts (Ts3) to suppress the contact sensitivity response against the hapten 4-hydroxy-3-nitrophen
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Dissertations / Theses on the topic "Hybridoma's"

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Amribt, Zakaria. "Macroscopic modelling of hybridoma cell fed-batch cultures with overflow metabolism: model-based optimization and state estimation." Doctoral thesis, Universite Libre de Bruxelles, 2014. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/209279.

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Monoclonal antibodies (MAbs) have an expanding market for use in diagnostic and therapeutic applications. Industrial production of these biopharmaceuticals is usually achieved based on fed-batch cultures of mammalian cells in bioreactors (Chinese hamster ovary (CHO) and Hybridoma cells), which can express different kinds of recombinant proteins. In order to reach high cell densities in these bioreactors, it is necessary to carry out an optimization of their production processes. Hence, macroscopic model equations must be developed to describe cell growth, nutrient consumption and product gener
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Pullen, A. M. "Studies on Peyer's patch T cell hybridomas." Thesis, University of Cambridge, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.233317.

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The initial objective of this thesis was to generate Peyer's patch T cell hybridomas producing lymphokines that regulate IgA-secreting B lymphocytes. Unprimed Peyer's patch cells were fused with BW5147. Karyotype analysis and fluorescent staining of the thy-1.2 marker confirmed the generation of hybridomas. It was envisaged that these hybridomas would be tested for their effects on IgA production by LPS-stimulated B cells. However, when the panel of hybridomas was available for testing there were technical difficulties with this assay. Sendai virus-primed Peyer's patch T cells were used in a s
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Kenney, John Stephen. "Functional antibody diversity of B-Lymphocyte hybridomas." Strasbourg 1, 1996. http://www.theses.fr/1996STR12363.

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蔡琼華 and King-wa Choi. "Detection and Identification of Salmonella Using Murine Hybridoma Monoclonal antibodies." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1994. http://hub.hku.hk/bib/B31211355.

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Choi, King-wa. "Detection and Identification of Salmonella Using Murine Hybridoma Monoclonal antibodies /." [Hong Kong : University of Hong Kong], 1994. http://sunzi.lib.hku.hk/hkuto/record.jsp?B13793834.

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Muller, Benjamin. "Création d'une banque de scFv-phages ciblant des protéines hydrophiles ou membranaires." Thesis, Montpellier 1, 2014. http://www.theses.fr/2014MON13511.

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Actuellement, 60% des médicaments sur le marché ont pour cible des protéines membranaires. Toutefois, l'étude de ces protéines membranaires reste un challenge de par leur structure particulière (domaines transmembranaires hydrophobes et domaines extra- et intra-cellulaires hydrophiles), mais également par leur faible expression sur les cellules.L'entreprise Ciloa, dans laquelle j'ai effectué ma thèse, a développé une technologie brevetée, qui permet d'exprimer à la surface des exosomes, des vésicules membranaires de tailles comprises entre 30 et 100nm, des protéines membranaires natives, grâce
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Godwin, Aella Natasha. "Enhanced production considering temperature effects on new hybridomas for bovine natural killer cell monoclonal antibodies." Pullman, Wash. : Washington State University, 2008. http://www.dissertations.wsu.edu/Thesis/Fall2008/A_Godwin_112008.pdf.

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Thesis (M.S. in chemical engineering)--Washington State University, December 2008.<br>Title from PDF title page (viewed on Mar. 4, 2009). "School of Chemical Engineering and Bioengineering." Includes bibliographical references.
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Wright, Kevin Ian Trevor. "Dual hollow fibre bioreactor for the growth of hybridomas." Thesis, University of Edinburgh, 1996. http://hdl.handle.net/1842/11611.

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The use of hollow fibre bioreactors in mammalian cell culture has provided a means by which large populations of viable cells can be grown continuously, for extended periods, in a relatively small volume of vessel. Bioreactors in which one set of fibres are used for nutrient and metabolite exchange from the cell mass are limited by their reliance on an axial flow regime. This pressure mediated flow pattern leads to the formation of nutrient gradients within the cell mass, which occur due to the large diffusional distances present in the growth space, resulting in cell death within the bioreact
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Wilson, James Samuel. "Process intensification of hybridoma cell fermentation." Thesis, University of Edinburgh, 1992. http://hdl.handle.net/1842/12155.

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Monoclonal antibodies can be produced in culture fluid by the fermentation of specificially selected hybridoma cells. Hybridoma cells exhibit suspension type fermentation characteristics and therefore the simplest method for large scale fermentation is that of the stirred tank fermenter. However, such is the growing demand for monoclonal antibodies, methods for increasing the production capacity of a commercial process are being developed. This study examines some of the current process intensification methods in relation to an established production facility. As well as examining the actual p
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Broadhurst, Edward Ross. "The effect of L-glutamate on growth of CC9C10 hybridomas." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/mq23233.pdf.

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Books on the topic "Hybridoma's"

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1946-, Taussig Michael J., ed. T cell hybridomas. CRC Press, 1985.

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J, Taussig Michael, ed. T cell hybridomas. CRC Press, 1985.

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Engleman, Edgar G., Steven K. H. Foung, James W. Larrick, and Andrew A. Raubitschek, eds. Human Hybridomas and Monoclonal Antibodies. Springer US, 1985. http://dx.doi.org/10.1007/978-1-4684-4949-5.

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G, Engleman Edgar, ed. Human hybridomas and monoclonal antibodies. Plenum Press, 1985.

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1944-, Strelkauskas Anthony J., ed. Human hybridomas: Diagnostic and therapeutic applications. M. Dekker, 1987.

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H, Bazin, ed. Rat hybridomas and rat monoclonal antibodies. CRC Press, 1990.

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Robert, Hay, ed. Catalogue of cell lines and hybridomas. 6th ed. American Type Culture Collection, 1988.

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Bartal, Arie H., and Yashar Hirshaut, eds. Methods of Hybridoma Formation. Humana Press, 1987. http://dx.doi.org/10.1007/978-1-4612-4826-2.

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Borrebaeck, Carl A. K., and Inger Hagen, eds. Electromanipulation in Hybridoma Technology. Palgrave Macmillan UK, 1989. http://dx.doi.org/10.1007/978-1-349-11339-2.

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H, Bartal Arie, and Hirshaut Yashar, eds. Methods of hybridoma formation. Humana Press, 1987.

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Book chapters on the topic "Hybridoma's"

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Gooch, Jan W. "Hybridomas." In Encyclopedic Dictionary of Polymers. Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_13957.

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Kozbor, Danuta, and Carlo M. Croce. "Human Hybridomas." In Methods of Hybridoma Formation. Humana Press, 1987. http://dx.doi.org/10.1007/978-1-4612-4826-2_15.

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Sogn, John A. "Interspecific Hybridomas." In Methods of Hybridoma Formation. Humana Press, 1987. http://dx.doi.org/10.1007/978-1-4612-4826-2_17.

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Burns, Robert. "Making Hybridomas." In Springer Protocols Handbooks. Humana Press, 2009. http://dx.doi.org/10.1007/978-1-59745-198-7_199.

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Entrican, Gary, Catherine Jepson, and David Deane. "Growing Hybridomas." In Springer Protocols Handbooks. Humana Press, 2009. http://dx.doi.org/10.1007/978-1-59745-198-7_200.

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Burns, Robert. "Making Hybridomas." In Immunochemical Protocols. Humana Press, 2005. http://dx.doi.org/10.1385/1-59259-873-0:041.

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Entrican, Gary, and Gareth Young. "Growing Hybridomas." In Immunochemical Protocols. Humana Press, 2005. http://dx.doi.org/10.1385/1-59259-873-0:055.

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Gooch, Jan W. "Hybridoma." In Encyclopedic Dictionary of Polymers. Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_13956.

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Gorevic, Peter D. "Immunochemistry of Hybridomas." In Radiolabeled Monoclonal Antibodies for Imaging and Therapy. Springer US, 1988. http://dx.doi.org/10.1007/978-1-4684-5538-0_1.

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Baron, D., J. H. Peters, R. K. H. Gieseler, et al. "Production of Hybridomas." In Monoclonal Antibodies. Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-74532-4_6.

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Conference papers on the topic "Hybridoma's"

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Moffat, E. H., R. H. Furlong, A. L. Bloom, and J. C. Giddings. "A MURINE MODEL FOR FACTOR VIII ANTIBODY ANTI-IDIOTYPE REAGENTS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644030.

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The regulation of factor VIII antibody (FVIIIAb) production in haemophilic and non-haemophilic patients may be effected by anti-idiotype (Aid) antibodies which specifically react with FVIIIAb. Aid antibodies (reagents) were prepared from rabbits immunised with murine monoclonal FVIIIAb. Immuno fluorescent microscopy and cell culture studies were performed using murine hybridoma cells which secreted the FVIIIAbs.Immuno fluorescence studies examined the ability of the Aid reagents to bind to acetone fixed FVIIIAb secreting hybridoma cells. Positive surface membrane and intra-cytoplasmic staining
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Furihata, Kenichi, Diane J. Nugent, Amy L. Bissonette, Elizabeth Vokac, and Thomas J. Kunicki. "PRODUCTION OF HUMAN MONOCLONAL ANTIBODIESSPECIFIC FOR PLATELET MEMBRANE GLYCOPROTEIN IIIa." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643705.

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Human monoclonal antibodies specific for platelet membrane glycoproteins (GPs) arepotentially important reagentsfor studies of the immunogenicity of membrane glycoproteins. A human monoclonalautoantibody, 5E5, reactive with plateletGPIIIa has been developed (Nugent, et al.,Blood, 1987, in press). In this report, we describe the production of additional human monoclonal antibodies specific for GPIIIa. Peripheral blood lymphocytes fromone patient with post-transfusion purpur(PTP) and one woman who had delivered an infant with neonatal alloimmune thrombocytopenic purpura (NATP) were used as a sou
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Oakley, Barbara A., Brooks A. Gross, Sara G. Martens, Darrin M. Hanna, and Gabrielle A. Stryker. "Electroporation-Induced Cell Lysis in SWLA-2 Hybridomas." In Conference Proceedings. Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE, 2006. http://dx.doi.org/10.1109/iembs.2006.259722.

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Oakley, Barbara A., Brooks A. Gross, Sara G. Martens, Darrin M. Hanna, and Gabrielle A. Stryker. "Electroporation-Induced Cell Lysis in SWLA-2 Hybridomas." In Conference Proceedings. Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE, 2006. http://dx.doi.org/10.1109/iembs.2006.4398199.

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Yasukawa, T., M. Suzuki, and F. Mizutani. "BS7.1 - Rapid formation of single-cell pairs for producing hybridomas." In 17th International Meeting on Chemical Sensors - IMCS 2018. AMA Service GmbH, Von-Münchhausen-Str. 49, 31515 Wunstorf, Germany, 2018. http://dx.doi.org/10.5162/imcs2018/bs7.1.

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Dewasme, Laurent, and Alain Vande Wouwer. "Dynamic modeling of sequential batch cultures of hybridoma cells." In 2016 International Conference on System Science and Engineering (ICSSE). IEEE, 2016. http://dx.doi.org/10.1109/icsse.2016.7551623.

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Zhang, Wei K., Ren Li, Zhi G. Fang, Zhi Y. Hu, Ze W. Wei, and Qin Li. "A Microfluidic Chip for Screening High-Producing Hybridomas at the Single-Cell Level." In 2019 IEEE 32nd International Conference on Micro Electro Mechanical Systems (MEMS). IEEE, 2019. http://dx.doi.org/10.1109/memsys.2019.8870728.

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Oakley, B. A., D. M. Hanna, S. Kandlikar, B. Gross, and G. A. Stryker. "Cell Lysis in SWLA-2 Hybridomas due to 1 kHz AC Electric Fields." In 2005 IEEE Engineering in Medicine and Biology 27th Annual Conference. IEEE, 2005. http://dx.doi.org/10.1109/iembs.2005.1616656.

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Heaton, Sam, Sofia Koch, Jiangyan Ge, et al. "Abstract 2164: In-licensing and knockout validation of monoclonal hybridomas to improve antibody reproducibility." In Proceedings: AACR Annual Meeting 2019; March 29-April 3, 2019; Atlanta, GA. American Association for Cancer Research, 2019. http://dx.doi.org/10.1158/1538-7445.sabcs18-2164.

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Heaton, Sam, Sofia Koch, Jiangyan Ge, et al. "Abstract 2164: In-licensing and knockout validation of monoclonal hybridomas to improve antibody reproducibility." In Proceedings: AACR Annual Meeting 2019; March 29-April 3, 2019; Atlanta, GA. American Association for Cancer Research, 2019. http://dx.doi.org/10.1158/1538-7445.am2019-2164.

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Reports on the topic "Hybridoma's"

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Jordan, Ramon L., Abed Gera, Hei-Ti Hsu, Andre Franck, and Gad Loebenstein. Detection and Diagnosis of Virus Diseases of Pelargonium. United States Department of Agriculture, 1994. http://dx.doi.org/10.32747/1994.7568793.bard.

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Pelargonium (Geranium) is the number one pot plant in many areas of the United States and Europe. Israel and the U.S. send to Europe rooted cuttings, foundation stocks and finished plants to supply a certain share of the market. Geraniums are propagated mainly vegetatively from cuttings. Consequently, viral diseases have been and remain a major threat to the production and quality of the crop. Among the viruses isolated from naturally infected geraniums, 11 are not specific to Pelargonium and occur in other crops while 6 other viruses seem to be limited to geranium. However, several of these v
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