Relevant bibliographies by topics / Hybridoma technology

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers

Academic literature on the topic 'Hybridoma technology'

Author: Grafiati
Published: 4 June 2021
Last updated: 26 July 2025

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Journal articles on the topic "Hybridoma technology"

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Vaghela, Arunkumar Ramjibhai, and Tejas H. Ganatra. "A detailed review of immunotherapeutics with a special emphasis on hybridoma technology." American Journal of Biopharmacy and Pharmaceutical Sciences 4 (January 25, 2024): 2. http://dx.doi.org/10.25259/ajbps_13_2023.

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The paper offers a thorough analysis of immunotherapeutics with a focus on hybridomas. It describes how focused and precise treatments for a variety of illnesses, such as cancer, autoimmune disorders, and infectious diseases, have been made possible by immunotherapeutics, which are based on antibody and hybridoma technology. The main therapeutics produced by this method are monoclonal antibodies (mAbs). The article describes the hybridoma technology process, in which a heterogeneous population of cells that produce unique mAbs are created by combining immortalized myeloma cells with B lymphocy
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Heilmann, Katja, and Pamela Holzlöhner. "Generation of antigen-specific monoclonal antibodies by in vitro immunization (TECH2P.904)." Journal of Immunology 194, no. 1_Supplement (2015): 206.14. http://dx.doi.org/10.4049/jimmunol.194.supp.206.14.

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Abstract Monoclonal antibodies are one of the most important tools in biomedicine but the generation by hybridoma technology is very time consuming and laborious. The adaptation of the process to an in vitro approach is therefore a smart alternative to reduce the disadvantages of hybridoma technology. In this study a protocol for in vitro immunization was developed which enables hematopoietic stem cells to differentiate into naïve dendritic cells. These cells were specifically activated with the antigen of interest and cocultivated with naïve B and T lymphocytes in order to generate antigen sp
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Shevchuk, Kateryna, Anastasia Baranovska, Andrii Chernetskyi, Nataliia Shchotkina, and Alexander Besarab. "Biosafety Aspects of Hybridoma Technology: Nature of Risks and Approaches to Their Management." Innovative Biosystems and Bioengineering 9, no. 2 (2025): 29–41. https://doi.org/10.20535/ibb.2025.9.2.320712.

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This study investigates the biosafety aspects of hybridoma technology, focusing on the identification and management of associated risks. Monoclonal antibodies, essential tools in immunology, biotechnology, and medicine, are primarily produced through hybridoma technology. This process involves fusing B lymphocytes from immunized animals with myeloma cells to create hybridomas, which are then cultured to produce specific antibodies. The research highlights significant contamination risks, particularly from rodent-borne viruses and other pathogens, during both in vivo and in vitro cultivation.
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Hidayati, Wahyu, Desti Hidayati, Pratiwi Pudjilestari Sudarmono, Tjahjani Mirawati Sudiro, Heri Wibowo, and Beti Ernawati Dewi. "Fully Human Monoclonal Antibodies Generated by Hybridoma Technology Blockade the Interaction of Spike Protein to Ace-2 Receptor." Journal of Advanced Research in Applied Sciences and Engineering Technology 62, no. 3 (2024): 118–29. https://doi.org/10.37934/araset.62.3.118129.

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Hybridoma technology has been used to generate monoclonal antibodies for both diagnostics and therapy. Furthermore, hybridoma technology can also be an alternative to produce human monoclonal antibodies for COVID-19 therapy. This study aimed to produce fully human-neutralized monoclonal antibodies for COVID-19. The fully human monoclonal antibodies were generated by fusion between human peripheral blood mononuclear cells (PBMC) and partner cells, K6H6/B5. The human-neutralized monoclonal antibodies were isolated and characterized by cloning dilution, recognition ability test and neutralization
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El Fitro, Sulis, Sutiman Bambang Sumitro, and Umie Lestari. "Development of Interactive Multimedia Based on Research of Hybridama Technology." Indonesian Journal of Science Learning (IJSL) 3, no. 1 (2022): 26–33. https://doi.org/10.15642/ijsl.v3i1.1984.

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The ability to understand and apply the current methods of Biotecnology as hybridoma technology needs to be taught to students. But in conducting laboratory activities hybridoma technology in the classroom activities takes time and costs are relatively large. This study was conducted to generate instructional media for students based research. The purpose of this research is to produce learning multimedia of hybridoma technology in Biotechnology course. This study uses the ADDIE development model. The results of this research is based on the feasibility studies show that interactive multimedia
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Moraes, Jane Zveiter, Bárbara Hamaguchi, Camila Braggion, et al. "Hybridoma technology: is it still useful?" Current Research in Immunology 2 (2021): 32–40. http://dx.doi.org/10.1016/j.crimmu.2021.03.002.

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Yagami, Hisanori, Hiroshi Kato, Kanta Tsumoto, and Masahiro Tomita. "Monoclonal antibodies based on hybridoma technology." Pharmaceutical Patent Analyst 2, no. 2 (2013): 249–63. http://dx.doi.org/10.4155/ppa.13.2.

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Pirofski, L., A. Casadevall, L. Rodriguez, L. S. Zuckier, and M. D. Scharff. "Current state of the hybridoma technology." Journal of Clinical Immunology 10, S6 (1990): 5S—14S. http://dx.doi.org/10.1007/bf00918686.

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Maier, Natalia, Pamela Holzloehner, Anja Hoenow, Astrid Scheunemann, Daniel Weschke, and Katja Hanack. "Characterization of monoclonal antibodies generated by in vitro immunization." Journal of Immunology 196, no. 1_Supplement (2016): 209.25. http://dx.doi.org/10.4049/jimmunol.196.supp.209.25.

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Abstract Monoclonal antibodies are highly valuable tools in biomedicine but the generation by hybridoma technology is very time-consuming and elaborate. In order to circumvent the consisting drawbacks an in vitro immunization approach was established by which murine as well as human monoclonal antibodies against a viral coat protein could be developed. The in vitro immunization process was performed by isolation of murine hematopoietic stem cells or human monocytes and an in vitro differentiation into immature dendritic cells. After antigen loading the cells were co-cultivated with naive T and
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Fan, Guo Ying, and Jin Qing Jiang. "Preparation and Immunological Traits of Monoclonal Antibody against Sarafloxacin." Advanced Materials Research 459 (January 2012): 54–57. http://dx.doi.org/10.4028/www.scientific.net/amr.459.54.

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Through cell fusion technology, five hybridoma lines of sarafloxacin (SAR), named S1-B2, S2-C6, S2-E7, S3-C5, and S3-E5, were identified and their corresponding mAbs were of the IgG1 isotype with a k light chain. The Kaffs of all mAbs were between 2.8 and 4.6×109 L/mol. The titers and IC50 values of purified ascite fluids were in the range of 0.512–2.56×106 and 0.32–0.48 ng/mL, respectively. The performances of S1-B2 and S2-C6 were more consistent in the stability experiments. Based on the S1-B2 hybridoma, an icELISA method was developed. The dynamic range was from 0.004 to 18 ng/mL, with a de
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Dissertations / Theses on the topic "Hybridoma technology"

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Minter, Ralph. "Development of antibody technology to identify natural killer cell surface antigens in Xenopus laevis." Thesis, Durham University, 1999. http://etheses.dur.ac.uk/4598/.

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Natural killer (NK)-like lymphocytes have recently been identified in thymectomised (Tx) Xenopus which are capable of spontaneous cytotoxicity towards the MHC- deficient, allogeneic thymus tumour cell line B(_3)B(_7). This Thesis describes attempts to raise antibodies to Xenopus NK cell surface antigens by phage display and hybridoma technology. The phage display technique was optimised for raising antibodies to novel, cellular antigens in a trial run using the Xenopus thymus tumour cell line B(_3)B(_7). Having isolated a phage antibody which was shown by flow cytometry to bind B(_3)B(_7) cell
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Ogunwumi, Olumide Babatope. "The Development of Monoclonal Antibodies Against Human Immunodeficiency Virus-1 Viral Protein R Using Hybridoma Technology." Youngstown State University / OhioLINK, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1441377756.

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Iddon, Dale. "Application of hybridoma technology to the study of West African Echis carinatus (Carpet viper) venom poisoning." Thesis, University of Liverpool, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.303688.

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Šupák, Marek. "Příprava myších monoklonálních protilátek proti cyklin-dependentní kináze 13." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2019. http://www.nusl.cz/ntk/nusl-401868.

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The aim of this master‘s thesis is to prepare a monoclonal antibody against cyclin-dependent kinase 13 (CDK13). The theoretical part focuses on antigen-antibody binding, which is essential for the use of monoclonal antibodies in the determination of CDK13 as well as the transcription that this kinase affects. This section is also devoted to Western blot and ELISA methods for detection of newly generated antibodies. Furthermore, the antibodies and the antigen definition are stated, which are later on discussed. The practical part is devoted to the preparation of antigen - its isolation and puri
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Hossain, Ishrat. "Preparation of monoclonal antibodies against immunoglobulin kappa of AL-amyloidosis and characterization of antibody producing hybridoma cells." Thesis, Uppsala universitet, Institutionen för kvinnors och barns hälsa, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-334412.

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Шебедя, Дмитро Сергійович. "Технологія виробництва субстанції моноклональних антитіл, специфічних до білку HBsAg вірусу гепатиту B. Дільниця культивування". Bachelor's thesis, КПІ ім. Ігоря Сікорського, 2020. https://ela.kpi.ua/handle/123456789/41083.

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Дипломний проєкт: 135 с., 26 рис., 12 табл., 13 формул, 90 посилань. Робота присвячена уніфікації та вдосконаленню технології масового напрацювання виробництва моноклональних антитіл у біореакторі, шляхом іммобілізації клітин продуцента на поверхні напівпроникних мембран та перфузійного типу подачі поживного середовища. На основі порівняльних характеристик, основним продуцентом було обрано найбільш стабільний та високо імуногенний штам гібридомних клітин, отриманих шляхом злиття клітин мієломи та спленоцитів мишачого походження. Штам 95E1 характеризується титром 1:1000 у культур
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Geaugey, Valéry. "Réacteurs membranaires pour la production continue d'anticorps monoclonaux : cinétique, performances et évaluation indirecte de la population cellulaire." Vandoeuvre-les-Nancy, INPL, 1991. http://www.theses.fr/1991INPL052N.

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Le but de ce travail est d'examiner les potentialités, faciliter la mise en œuvre et mieux comprendre le fonctionnement des réacteurs membranaires, qui pourraient s'avérer plus performants que les réacteurs agités majoritairement employés actuellement pour la culture de cellules animales. Les effets de concentrations en nutriments et produits toxiques, de variations du ph et de l'osmolarité sur les cinétiques de croissance cellulaire, de consommation et de production sont étudiés. Ensuite, le problème du suivi de la biomasse en réacteurs membranaires est abordé. Après une analyse critique de l
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Vin-Capiaumont, Josette. "Une expérience en culture de cellules animales : à propos de la fabrication d'hybridomes et de la production en masse d'anticorps monoclonaux. Essais d'amélioration de la technologie." Nancy 1, 1992. http://docnum.univ-lorraine.fr/public/SCD_T_1992_0257_CAPIAUMONT.pdf.

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L'objectif principal de ce travail est l'étude des différentes étapes de la fabrication de souches d'hybridomes et de la culture en masse des anticorps monoclonaux produits. Après une description détaillée des techniques utilisées, sont présentés les résultats des fusions réalisées après immunisation (par plusieurs antigènes) des lymphocytes de souris soit in vivo soit in vitro. Ensuite sont expliqués la sélection et les différents modes de culture et de sauvegarde de ces souches d'hybridomes obtenues: anti beta lactoglobuline, anti alpha 1 antitrypsine et anti hormone lactogène placentaire. L
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吳宜欣. "Improvement of hybridoma technology to enhance the efficiency of hybridoma preparation." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/343876.

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Costa, Tiago José Alexandre da Silva. "Characterization of novel antibodies and glycan binding proteins against cancer." Master's thesis, 2018. http://hdl.handle.net/10362/76370.

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Cancer is the largest cause of death worldwide and it is increasingly urgent to develop new approaches to diagnosis and therapy. The glycosylation pattern of the cancer cells differ from that of healthy cells and therefore the aberrant expression of glycans are promising targets for diagnosis and therapy. Sialyl-Tn (STn) is a glycan, whose expression is increased in cancer cells and almost absent in normal cells. It is involved in processes such as cancer growth, progression and metastasis. In addition to STn other glycans present an aberrant pattern in cancer. As is the case of Sialyl Lewis
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Books on the topic "Hybridoma technology"

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Borrebaeck, Carl A. K., and Inger Hagen, eds. Electromanipulation in Hybridoma Technology. Palgrave Macmillan UK, 1989. http://dx.doi.org/10.1007/978-1-349-11339-2.

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Springer, Timothy A., ed. Hybridoma Technology in the Biosciences and Medicine. Springer US, 1985. http://dx.doi.org/10.1007/978-1-4684-4964-8.

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A, Springer Timothy, ed. Hybridoma technology in the biosciences and medicine. Plenum Press, 1985.

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International Symposium on In Vitro Immunization in Hybridoma Technology (1987 Tylösand, Sweden). In vitro immunization in hybridoma technology: Proceedings of the International Symposium on In Vitro Immunization in Hybridoma Technology, Tylösand, Sweden, September 7-8, 1987. Elsevier, 1988.

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International Congress on Rapid Methods and Automation in Microbiology and Immunology (7th 1993 London, England). Rapid methods and automation in microbiology and immunology: RAMI-93. Intercept, 1994.

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Borrebaeck, Carl A. K., and Inger Hagen. Electromanipulation in Hybridoma Technology. Palgrave Macmillan, 1990.

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Borrebaeck, Carl A. K., and Inger Hagen. Electromanipulation in Hybridoma Technology. Palgrave Macmillan, 1990.

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International Symposium on In Vitro Immunization in Hybridoma Technology (1987 Tylösand, Sweden). In vitro immunization in hybridoma technology. Elsevier, 1988.

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Borrebaeck, Carl A. K. Electromanipulation in Hybridoma Technology: A Laboratory Manual. Oxford Univ Pr, 1989.

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Carl A.K. Borrebaeck (Editor) and Inger Hagen (Editor), eds. Electromanipulation in Hybridoma Technology: A Laboratory Manual. Oxford University Press, USA, 1989.

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Book chapters on the topic "Hybridoma technology"

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Hnasko, Robert M., and Larry H. Stanker. "Hybridoma Technology." In Methods in Molecular Biology. Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2742-5_2.

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Betsy, C. Judith, and C. Siva. "Hybridoma Technology." In Fisheries Biotechnology and Bioinformatics. Springer Nature Singapore, 2023. http://dx.doi.org/10.1007/978-981-99-6991-3_12.

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Olsson, Lennart, and Peter Brams. "Human—Human Hybridoma Technology." In Human Hybridomas and Monoclonal Antibodies. Springer US, 1985. http://dx.doi.org/10.1007/978-1-4684-4949-5_13.

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Wiggenhauser, A., J. H. Peters, and H. Baumgarten. "Preconditions for Hybridoma Technology." In Monoclonal Antibodies. Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-74532-4_2.

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Andreeff, Michael, and Edith Espiritu. "Flow Cytometry in Hybridoma Technology." In Methods of Hybridoma Formation. Humana Press, 1987. http://dx.doi.org/10.1007/978-1-4612-4826-2_24.

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Zimmermann, Ulrich, Petra Gessner, Michaela Wander, and Steven K. H. Foung. "Electroinjection and Electrofusion in Hypo-osmolar Solution." In Electromanipulation in Hybridoma Technology. Palgrave Macmillan UK, 1989. http://dx.doi.org/10.1007/978-1-349-11339-2_1.

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Jonak, Zdenka L., Patrick Machy, Edward Henri, et al. "Electroporation of Human Lymphoid Cells." In Electromanipulation in Hybridoma Technology. Palgrave Macmillan UK, 1989. http://dx.doi.org/10.1007/978-1-349-11339-2_2.

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Perkins, Susan, Ulrich Zimmermann, Petra Gessner, and Steven K. H. Foung. "Formation of Hybridomas Secreting Human Monoclonal Antibodies with Mouse-Human Fusion Partners." In Electromanipulation in Hybridoma Technology. Palgrave Macmillan UK, 1989. http://dx.doi.org/10.1007/978-1-349-11339-2_3.

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Mally, Martin I., and Mark C. Glassy. "Generating Immortalized Immunoglobulin-secreting Human Lymphocytes by Recombinant DNA Technology." In Electromanipulation in Hybridoma Technology. Palgrave Macmillan UK, 1989. http://dx.doi.org/10.1007/978-1-349-11339-2_4.

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Conrad, Mary K., and Mathew M. S. Lo. "B-Cell Hybridoma Production by Avidin-Biotin Mediated Electrofusion." In Electromanipulation in Hybridoma Technology. Palgrave Macmillan UK, 1989. http://dx.doi.org/10.1007/978-1-349-11339-2_5.

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Conference papers on the topic "Hybridoma technology"

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Shukla, Girja S., Stephanie C. Pero, Yu-Jing Sun, Chelsea Carman, and David N. Krag. "Abstract 4736: Generation of tumor-specific antibodies from sentinel lymph nodes of breast cancer patients using hybridoma technology." In Proceedings: AACR 104th Annual Meeting 2013; Apr 6-10, 2013; Washington, DC. American Association for Cancer Research, 2013. http://dx.doi.org/10.1158/1538-7445.am2013-4736.

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Su, Jixiang, Lifeng Zhang, Chao Xu, and Tao He. "Guided-TargetTree-Hybrid_A* Path Planning Algorithm for Vertical Parking." In 2023 3rd International Conference on Computer Science, Electronic Information Engineering and Intelligent Control Technology (CEI). IEEE, 2023. http://dx.doi.org/10.1109/cei60616.2023.10527966.

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Thi Kim, Hong Tran, and Trang Nguyen Huu Phuoc. "Sprouting Buds of Petunia Hybrida Cuttings Under Normal Conditions in Vitro Culture." In 2023 8th International Scientific Conference on Applying New Technology in Green Buildings (ATiGB). IEEE, 2023. http://dx.doi.org/10.1109/atigb59969.2023.10364343.

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