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1

Truskewycz, Adam, Taylor D. Gundry, Leadin S. Khudur, Adam Kolobaric, Mohamed Taha, Arturo Aburto-Medina, Andrew S. Ball, and Esmaeil Shahsavari. "Petroleum Hydrocarbon Contamination in Terrestrial Ecosystems—Fate and Microbial Responses." Molecules 24, no. 18 (September 19, 2019): 3400. http://dx.doi.org/10.3390/molecules24183400.

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Petroleum hydrocarbons represent the most frequent environmental contaminant. The introduction of petroleum hydrocarbons into a pristine environment immediately changes the nature of that environment, resulting in reduced ecosystem functionality. Natural attenuation represents the single, most important biological process which removes petroleum hydrocarbons from the environment. It is a process where microorganisms present at the site degrade the organic contaminants without the input of external bioremediation enhancers (i.e., electron donors, electron acceptors, other microorganisms or nutrients). So successful is this natural attenuation process that in environmental biotechnology, bioremediation has developed steadily over the past 50 years based on this natural biodegradation process. Bioremediation is recognized as the most environmentally friendly remediation approach for the removal of petroleum hydrocarbons from an environment as it does not require intensive chemical, mechanical, and costly interventions. However, it is under-utilized as a commercial remediation strategy due to incomplete hydrocarbon catabolism and lengthy remediation times when compared with rival technologies. This review aims to describe the fate of petroleum hydrocarbons in the environment and discuss their interactions with abiotic and biotic components of the environment under both aerobic and anaerobic conditions. Furthermore, the mechanisms for dealing with petroleum hydrocarbon contamination in the environment will be examined. When petroleum hydrocarbons contaminate land, they start to interact with its surrounding, including physical (dispersion), physiochemical (evaporation, dissolution, sorption), chemical (photo-oxidation, auto-oxidation), and biological (plant and microbial catabolism of hydrocarbons) interactions. As microorganism (including bacteria and fungi) play an important role in the degradation of petroleum hydrocarbons, investigations into the microbial communities within contaminated soils is essential for any bioremediation project. This review highlights the fate of petroleum hydrocarbons in tertial environments, as well as the contributions of different microbial consortia for optimum petroleum hydrocarbon bioremediation potential. The impact of high-throughput metagenomic sequencing in determining the underlying degradation mechanisms is also discussed. This knowledge will aid the development of more efficient, cost-effective commercial bioremediation technologies.
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2

Vane, Ronald, and Vince Carlino. "Environmental Contamination Sources and Control in High Resolution Scanning Electron Microscopy." Microscopy Today 14, no. 4 (July 2006): 62–63. http://dx.doi.org/10.1017/s155192950005032x.

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Traditionally, contamination control in SEMs has focused on pump oils, finger prints, dirty specimens, and good vacuum practice in manufacturing. Now, the use of dry pumps at all stages of the vacuum system of new FE SEMs, and the use of better vacuum practices on the part on users and manufacturers have made environmental hydrocarbons, the hydrocarbon background contamination of our world, a significant source of the remaining hydrocarbons in electron microscope vacuum systems. These environment sources of hydrocarbons (HC) cause a loss of resolution and contrast in imaging at the highest levels of magnification.
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3

Al-Hassen, Shukri I., Hamid T. Al-Saad, and Dawod J. Al-Rubaiay. "An Analytical Study on Petroleum Hydrocarbons Contamination in the Urban Environment of Basra City, Southern Iraq." Journal of Petroleum Research and Studies 4, no. 2 (September 1, 2013): 12–29. http://dx.doi.org/10.52716/jprs.v4i2.97.

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The present study aims to analyze the spatial and seasonal variations in levels of petroleum hydrocarbons at the urban environment of Basra City. This is made by determination of their concentrations in water, ambient air, and soils. Several samples were collected from different sampling stations during 2009. The determination of hydrocarbons in water samples was carried out using the procedure of UNESCO, and the hydrocarbons in ambient air were measured by the portable gas detector of Drager CMS, whereas the determination of hydrocarbons in soils was conducted as described in Al-Saad. The findings demonstrated that seasonal variations in hydrocarbon concentrations which were increased during winter for both water and soil samples, while increased during summer for ambient air samples. Moreover, there were spatial variations in hydrocarbon concentrations which were significantly higher at the sampling stations located within the urban center in comparison with those in the suburbs. The recorded values were ranged from 0.11 to 190.5 µg/l in water samples, from ≥ 20 to 29.3 ppm in ambient air samples, and from 0.56 to 41.58 µg/gmdry weight in soil samples. Some concentrations lies within high levels of exposure, which may give rise to adverse health consequences.
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4

Onome Augustina Bubu-Davies, Benjamin Bameyi Otene, and Mpakaboari Vellington Cephas Ebini. "Polycyclic aromatic hydrocarbon contamination in water, sediments and aquatic life of Nigerian inland and coastal waters." Magna Scientia Advanced Research and Reviews 1, no. 3 (March 30, 2021): 01–012. http://dx.doi.org/10.30574/msarr.2021.1.3.0014.

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formed during incomplete combustions of organic substances but few to be mention such as cigarettes, coal etc. They are usually found as a mixture containing two or more compounds such as soot. The emissions of PAHs in Nigeria have contributed significantly to the environment and live of aquatic organisms. Thus, this paper reviewed the contamination of polycyclic aromatic hydrocarbon (PAHs) in the water, sediments and organisms in inland and coastal waters. Methodology: Literatures of relevant and previous studies of polycyclic aromatic hydrocarbons in the water, sediment and organism within and outside Nigeria were reviewed. Results: The contamination of polycyclic aromatic hydrocarbons (PAHs) was known to be carcinogenic, mutagenic, teratogenic and can cause adverse effect on human health, wildlife and aquatic lives with no report on mammals in the aquatic environments. Conclusion and Recommendation: Polycyclic aromatic hydrocarbons (PAHs) reviewed displayed different effects caused in the lives of human and aquatic organism based on the concentration level. Their sources were more of anthropogenic than natural source with varied concentrations at various source points due to different activities in question. The positive impact of polycyclic aromatic hydrocarbon on fish and other aquatic organisms as a result of bioconcentration, biotransformation and biomagnification become a threat to humans that rely on eighty percent of aquatic resources. Therefore, conceived efforts should be made to reduce these effects, general public monitoring of polycyclic aromatic hydrocarbon on discharge sources in the biosphere.
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5

Kmiecik, Natalia, Krzysztof Jurek, and Adam Kowalski. "Assessment of soil contamination by oil-derived compounds in the Kielce agglomeration using gas chromatography coupled with mass spectrometry (GC-MS)." E3S Web of Conferences 108 (2019): 02005. http://dx.doi.org/10.1051/e3sconf/201910802005.

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Soil contamination with petroleum-derived compounds is one of the most serious ecological problems. Their main source in urbanized areas is public transport. According to the Regulation of the Polish Minister of Environment about the assessment of earth’s surface contamination, hydrocarbon compounds are divided into: gasolines and oils, aromatic hydrocarbons and polycyclic aromatic hydrocarbons (PAHs). The subject of the research is the use of a gas chromatography coupled with mass spectrometry (GC-MS) method to assess the degree of soil contamination in the vicinity of the main communication routes of the Kielce agglomeration. This method is useful for the detection and identification of many organic compounds occurring in samples in very small quantities, which cannot be determined by common methods. For the purpose of this study, 14 soil samples were collected. After carrying out extraction with various methods, the ultrasonic solvent extraction method was chosen as the most effective way of extracting hydrocarbon impurities for the GC-MS studies. During single analysis, both oil fraction and PAHs quantities were determined.
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6

Brand, Vitali, Michael S. Baker, and Maarten P. de Boer. "Controlling Environment and Contact Materials to Optimize Ohmic Microrelay Lifetimes." MRS Proceedings 1659 (2014): 63–68. http://dx.doi.org/10.1557/opl.2014.181.

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ABSTRACTThere has been a recent resurgence in interest in developing ohmic switches to complement transistors in order to address challenges associated with electrical current leakage. A critical limitation in ohmic switches remains the reliability of their electrical contacts. These contacts are prone to hydrocarbon induced contamination which progressively inhibits signal transmission, eventually leading to device failure. We report on progress made towards controlling the contamination phenomenon. We discuss how contact materials and operating environment affect device performance, showing that RuO2 coated microswitch contacts operating in the presence of O2 experience very limited contaminant accumulation even in hydrocarbon-rich environments. We then demonstrate that devices which have experienced contamination can recover their original performance by being operated in clean N2:O2 environment. Finally, we suggest that this resistance recovery is associated with the chemical transformation of the contaminant as opposed to its removal and that the transformed contaminant may shield the Pt coating from oxidation.
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7

Suárez-Moo, Pablo, Araceli Lamelas, Itza Garcia-Bautista, Luis Felipe Barahona-Pérez, Gloria Sandoval-Flores, David Valdes-Lozano, Tanit Toledano-Thompson, Erik Polanco-Lugo, and Ruby Valdez-Ojeda. "Characterization of sediment microbial communities at two sites with low hydrocarbon pollution in the southeast Gulf of Mexico." PeerJ 8 (December 8, 2020): e10339. http://dx.doi.org/10.7717/peerj.10339.

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Background Coastal ecosystems are prone to hydrocarbon pollution due to human activities, and this issue has a tremendous impact on the environment, socioeconomic consequences, and represents a hazard to humans. Bioremediation relies on the ability of bacteria to metabolize hydrocarbons with the aim of cleaning up polluted sites. Methods The potential of naturally occurring microbial communities as oil degraders was investigated in Sisal and Progreso, two port locations in the southeast Gulf of Mexico, both with a low level of hydrocarbon pollution. To do so, we determined the diversity and composition of bacterial communities in the marine sediment during the dry and rainy seasons using 16S rRNA sequencing. Functional profile analysis (PICRUTSt2) was used to predict metabolic functions associated with hydrocarbon degradation. Results We found a large bacterial taxonomic diversity, including some genera reported as hydrocarbon-degraders. Analyses of the alpha and beta diversity did not detect significant differences between sites or seasons, suggesting that location, season, and the contamination level detected here do not represent determining factors in the structure of the microbial communities. PICRUTSt2 predicted 10 metabolic functions associated with hydrocarbon degradation. Most bacterial genera with potential hydrocarbon bioremediation activity were generalists likely capable of degrading different hydrocarbon compounds. The bacterial composition and diversity reported here represent an initial attempt to characterize sites with low levels of contamination. This information is crucial for understanding the impact of eventual rises in hydrocarbon pollution.
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8

Faiza, Bendadeche Medjahed. "Hydrocarbon-Degrading Bacterial Strain Pseudomonas mendocina Newly Isolated from Marine Sediments and Seawater of Oran Harbor (Algerian Coast)." Archives of Ecotoxicology 2, no. 2 (June 3, 2020): 22–29. http://dx.doi.org/10.36547/ae.2020.2.2.22-29.

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Contamination by petroleum hydrocarbons causes serious dangers to human health and the environment, whether by accidental or chronic contamination. Due to the large flow of ships, the commercial harbor of Oran is subject to pollution particularly by polycyclic aromatic hydrocarbons. For that, bioremediation by indigenous microorganisms is the most important method to eliminate or decrease this contamination. In the present paper, hydrocarbon-degrading bacterium strain SP57N has been studied, newly isolated from contaminated marine sediments and sea water from the harbor of Oran (Northwestern-Algeria), using of Bushnell-Hass salt medium (BHSM). The strain SP57N was Gram-negative, oxidase negative, catalase negative, motile, Rod-shaped bacteria, identified molecularly as Pseudomonas mendocina based on partial 16S rDNA gene sequence analysis, using the BLAST program on National Centre for Biotechnology Information (NCBI) and the EzBioCloud 16S rDNA databases. This isolate could growth on high concentrations of crude oil (up to 10 %, v/v). The effects of some culture conditions such as temperature, NaCl concentration and pH on growth rate of strain SP57N on crude oil as the sole carbon and energy source were studied. In addition, growth kinetic of this isolate on crude oil during 20 days of culture at 140 rpm, under optimal culture conditions was considered. The results showed maximum growth rate at temperature 25°C, 3% (w/v) of NaCl concentration and pH 7. Results of growth kinetic on crude oil as sole carbon and energy source showed that the stationary phase was attained at day 12. Thus, Pseudomonas mendocina SP57N had effectively hydrocarbon-degrading potential, and could be used as an efficacy degrader to initiate a biological eco-friendly method for the bioremediation of the hydrocarbon pollution on the port of Oran, and marine environment.
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9

Thi Quynh Hoa, Kieu, Nguyen Vu Giang, Nguyen Thi Yen, Mai Duc Huynh, Nguyen Huu Dat, Vuong Thi Nga, Nguyen Thi Thu Ha, and Pham Thi Phuong. "Enhanced bioremediation of crude oil polluted water by a hydrocarbon-degrading Bacillus strain immobilized on polyurethane foam." Vietnam Journal of Biotechnology 18, no. 3 (November 28, 2020): 581–88. http://dx.doi.org/10.15625/1811-4989/18/3/15714.

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During the production and transportation of petroleum hydrocarbons, unsuitable operation and leakage may result in contamination of water and soil with petroleum hydrocarbons. Petroleum contamination causes significant marine environmental impacts and presents substantial hazards to human health. Bioremediation of contaminated water and soil is currently the effective and least harmful method of removing petroleum hydrocarbons from the environment. To improve the survival and retention of the bioremediation agents in the contaminated sites, microbial cells must be immobilized. It was demonstrated that immobilized microbial cells present advantages for degrading petroleum hydrocarbon pollutants compared to free suspended cells. In this study, the ability of a Bacillus strain (designed as Bacillus sp. VTVK15) to immobilize on PUF and to degrade crude oil was investigated. The immobilized Bacilllus strain had the highest number (5.38 ± 0.12 Í 108 CFU/g PUF) and a maximum attachment efficiency of 92% on PUF after 8 days. Analysis by GC-MS revealed that both free and immobilized cells of Bacillus sp. VTVK15 were able to degrade 65 and 90% of the hydrocarbons in 2% (v/v) crude oil tested after 14 days, respectively. The results suggest the potential of using PUF-immobilized Bacillus sp. VTVK15 to bioremediate petroleum hydrocarbons in an open marine environment.
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10

Arjoon, Karuna, and James G. Speight. "Chemical and Physical Analysis of a Petroleum Hydrocarbon Contamination on a Soil Sample to Determine Its Natural Degradation Feasibility." Inventions 5, no. 3 (August 20, 2020): 43. http://dx.doi.org/10.3390/inventions5030043.

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Crude oil is the world’s leading fuel source and is the lifeblood of the industrialized nations as it is vital to produce many everyday essentials. This dependency on fossil fuels has resulted in serious environmental issues in recent times. Petroleum contaminated soils must be treated to ensure that human health and the environment remain protected. The restoration of petroleum-polluted soil is a complex project because once petroleum hydrocarbon enters the environment, the individual constituents will partition to various environmental compartments in accordance with their own physical–chemical properties; therefore, the composition and inherent biodegradability of the petroleum hydrocarbon pollutant determines the suitability of a remediation approach. The objective of this study was to assess the prospective of bioremediation as a feasible technique for practical application to the treatment of petroleum hydrocarbon-contaminated soils, by trending the changes in the properties of the petroleum due to biodegradation. Each polluted soil has particularities, thus, the bioremediation approach for each contaminated site is unique. Therefore, hydrocarbon-contaminated sites that have remained polluted for decades due to lack of proper decontamination treatments present in this part of the world would benefit from cost effective treatments. Most bioremediation case studies are usually based on hypothetical assumptions rather than technical or experimental data; providing data that show the capabilities of biodegradation of indigenous microbes on specific oil composition can lead to the creation of strategies to accelerate the biological breakdown of hydrocarbons in soil.
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11

Putilina, V. S., I. V. Galitskaya, and T. I. Yuganova. "Plume of oil metabolites in groundwater: formation, evolution, and toxicity." Геоэкология. Инженерная геология. Гидрогеология. Геокриология, no. 1 (April 17, 2019): 38–45. http://dx.doi.org/10.31857/s0869-78092019138-45.

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The transformation of oil hydrocarbons in groundwater is mainly related to the processes of biodegradation. At sites where residual crude oil or petroleum hydrocarbon fuel contaminants are present in the environment, biodegradation reactions result in the formation of partial oxidation products, i.e., metabolites. These transformation products are more soluble than the parent petroleum hydrocarbons, due to their greater polarity and corresponding low volatility. Transformation products from residual source zones are distributed in aqueous phase to form a plume of contamination in groundwater. The content of metabolites depends on the redox conditions and the presence of the terminal electron acceptors, as well as on the structure of the original hydrocarbon compounds. The article considers the conditions for formation of metabolites, their degradation, migration to groundwater and plume formation, toxicity of metabolites. Examples of modeling the migration of metabolites in the saturated zone are given.
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12

Šyvokienė, Janina. "Changes in microbiota of rainbow trout caused by sediments contamination." Open Life Sciences 8, no. 12 (December 1, 2013): 1265–71. http://dx.doi.org/10.2478/s11535-013-0236-3.

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AbstractThe abundance, composition and hydrocarbon-degrading bacteria, as possible biomarkers of contamination with oil hydrocarbons, of autochthonous and alochtonous microbiota of the digestive tract of rainbow trout have been estimated. The samples of the bottom sediments for microbiological tests have been collected and a response of natural bacterial communities in the digestive tract of rainbow trout and nutritional changes has been investigated. Experimental fish have been fed with a mixture of three substances with the aim to assess the influence of hydrocarbon-degrading bacteria contained in the sediments on the microbiota of rainbow trout’s digestive tracts. The abundance values of rainbow trout intestinal heterotrophic bacteria were found to change depending on alochtonous microbiota of different bottom sediments given to the experimental fish with food in vitro. According to the results of our research, it is likely that the changes in the abundance values of the microbiota of the digestive tract of fish and in the proportions of functional groups of the bacteria allow us to determine changes in the functional activity of bacteria depending on food composition. Any relative increase or decrease of abundance or activity of alochtonous microbiota allows the prediction of toxic effects of the contaminants on animals and the environment.
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13

Rovere, Marzia, Alessandra Mercorella, Emanuela Frapiccini, Valerio Funari, Federico Spagnoli, Claudio Pellegrini, Andree Soledad Bonetti, et al. "Geochemical and Geophysical Monitoring of Hydrocarbon Seepage in the Adriatic Sea." Sensors 20, no. 5 (March 9, 2020): 1504. http://dx.doi.org/10.3390/s20051504.

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Hydrocarbon seepage is overlooked in the marine environment, mostly due to the lack of high-resolution exploration data. This contribution is about the set-up of a relocatable and cost-effective monitoring system, which was tested on two seepages in the Central Adriatic Sea. The two case studies are an oil spill at a water depth of 10 m and scattered biogenic methane seeps at a water depth of 84 m. Gas plumes in the water column were detected with a multibeam system, tightened to sub-seafloor seismic reflection data. Dissolved benthic fluxes of nutrients, metals and Dissolved Inorganic Carbon (DIC) were measured by in situ deployment of a benthic chamber, which was used also for the first time to collect water samples for hydrocarbons characterization. In addition, the concentration of polycyclic aromatic hydrocarbons, as well as major and trace elements were analyzed to provide an estimate of hydrocarbon contamination in the surrounding sediment and to make further inferences on the petroleum system.
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Das, Nilanjana, and Preethy Chandran. "Microbial Degradation of Petroleum Hydrocarbon Contaminants: An Overview." Biotechnology Research International 2011 (September 13, 2011): 1–13. http://dx.doi.org/10.4061/2011/941810.

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One of the major environmental problems today is hydrocarbon contamination resulting from the activities related to the petrochemical industry. Accidental releases of petroleum products are of particular concern in the environment. Hydrocarbon components have been known to belong to the family of carcinogens and neurotoxic organic pollutants. Currently accepted disposal methods of incineration or burial insecure landfills can become prohibitively expensive when amounts of contaminants are large. Mechanical and chemical methods generally used to remove hydrocarbons from contaminated sites have limited effectiveness and can be expensive. Bioremediation is the promising technology for the treatment of these contaminated sites since it is cost-effective and will lead to complete mineralization. Bioremediation functions basically on biodegradation, which may refer to complete mineralization of organic contaminants into carbon dioxide, water, inorganic compounds, and cell protein or transformation of complex organic contaminants to other simpler organic compounds by biological agents like microorganisms. Many indigenous microorganisms in water and soil are capable of degrading hydrocarbon contaminants. This paper presents an updated overview of petroleum hydrocarbon degradation by microorganisms under different ecosystems.
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15

Tan, Yew Ai, and Ainte Kuntom. "Hydrocarbons m Crude Palm Kernel Oil." Journal of AOAC INTERNATIONAL 77, no. 1 (January 1, 1994): 67–73. http://dx.doi.org/10.1093/jaoac/77.1.67.

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Abstract The sources of hydrocarbons in crude palm kernel oil were investigated by a series of laboratory-controlled oil extractions of kernels of varying quality. Site examinations of palm kernel-crushing plants were also conducted to determine possible sources of hydrocarbon contamination of palm kernels throughout the process of kernel extraction. Parallel to these studies, a random survey of crude palm kernel oil (CPKO) produced by different kernel crushers was also carried out to determine the range of hydrocarbon concentrations in locally produced CPKO. This study showed that hydrocarbons can be picked up from sources such as glassware, extracting apparatus, and plastic containers and stoppers. Extraction of oil from low-quality kernels that were both moldy and rancid, broken kernels, and kernels plus added shells also resulted in a higher hydrocarbon level in the final CPKO. Overheating and cooking of the kernels before extraction also contributed to the overall hydrocarbon content. The random survey of hydrocarbon level showed a range of 0.6–7.1 ppm.
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Margesin, R., D. Labb�, F. Schinner, C. W. Greer, and L. G. Whyte. "Characterization of Hydrocarbon-Degrading Microbial Populations in Contaminated and Pristine Alpine Soils." Applied and Environmental Microbiology 69, no. 6 (June 2003): 3085–92. http://dx.doi.org/10.1128/aem.69.6.3085-3092.2003.

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ABSTRACT Biodegradation of petroleum hydrocarbons in cold environments, including Alpine soils, is a result of indigenous cold-adapted microorganisms able to degrade these contaminants. In the present study, the prevalence of seven genotypes involved in the degradation of n-alkanes (Pseudomonas putida GPo1 alkB; Acinetobacter spp. alkM; Rhodococcus spp. alkB1, and Rhodococcus spp. alkB2), aromatic hydrocarbons (P. putida xylE), and polycyclic aromatic hydrocarbons (P. putida ndoB and Mycobacterium sp. strain PYR-1 nidA) was determined in 12 oil-contaminated (428 to 30,644 mg of total petroleum hydrocarbons [TPH]/kg of soil) and 8 pristine Alpine soils from Tyrol (Austria) by PCR hybridization analyses of total soil community DNA, using oligonucleotide primers and DNA probes specific for each genotype. The soils investigated were also analyzed for various physical, chemical, and microbiological parameters, and statistical correlations between all parameters were determined. Genotypes containing genes from gram-negative bacteria (P. putida alkB, xylE, and ndoB and Acinetobacter alkM) were detected to a significantly higher percentage in the contaminated (50 to 75%) than in the pristine (0 to 12.5%) soils, indicating that these organisms had been enriched in soils following contamination. There was a highly significant positive correlation (P < 0.001) between the level of contamination and the number of genotypes containing genes from P. putida and Acinetobacter sp. but no significant correlation between the TPH content and the number of genotypes containing genes from gram-positive bacteria (Rhodococcus alkB1 and alkB2 and Mycobacterium nidA). These genotypes were detected at a high frequency in both contaminated (41.7 to 75%) and pristine (37.5 to 50%) soils, indicating that they are already present in substantial numbers before a contamination event. No correlation was found between the prevalence of hydrocarbon-degradative genotypes and biological activities (respiration, fluorescein diacetate hydrolysis, lipase activity) or numbers of culturable hydrocarbon-degrading soil microorganisms; there also was no correlation between the numbers of hydrocarbon degraders and the contamination level. The measured biological activities showed significant positive correlation with each other, with the organic matter content, and partially with the TPH content and a significant negative correlation with the soil dry-mass content (P < 0.05 to 0.001).
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Ehiomogue, Precious, Israel Ahuchaogu, and Edwin Ahaneku. "A review of bioreremediation of hydrocarbon contaminated soils in Niger Delta area of Nigeria." Poljoprivredna tehnika 46, no. 3 (2021): 23–39. http://dx.doi.org/10.5937/poljteh2103023p.

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Bioremediation technologies are an environmentally friendly approach for the treatment of polluted soils. This review take a look at the various remediation efforts by various scientist to ameliorate the effect of crude oil contamination on the environment. Contamination of the total environment (air, soil, water and biota) by crude oil has become a paramount interest in the Niger Delta region of Nigeria. With the frequent reports of oil spillages in the Niger Delta area, there is need to seek for a cost effective method for remediation of crude oil impacted soils. Studies have revealed variable impacts of oil toxicity on the environment and exposed populations. Soil contamination is caused mainly by the leakage of underground storage tanks and pipes. The most common conventional method for the remediation of contaminated soils is excavation followed by landfilling or incineration and other technologies that have been widely practiced. Contaminated sites pose a threat to human life due to severe health problems caused by adverse health effects from exposure to soil contamination. Once it is being detected, assessment strategies, type of sampling, chemical analyses, evaluation of parameters and its effect must be done. Several technologies and parameters have been developed to treat petroleum hydrocarbon contaminated soil but the problem still exists.
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Bouwer, E. J., C. T. Chen, and Y. H. Li. "Transformation of a Petroleum Mixture in Biofilms." Water Science and Technology 26, no. 3-4 (August 1, 1992): 637–46. http://dx.doi.org/10.2166/wst.1992.0444.

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Petroleum hydrocarbons from a lagoon storing waste oil in New Jersey have contaminated underlying soil and groundwater. Biofilm column studies were performed to investigate biotransformation of the petroleum mixture under aerobic and anaerobic conditions. The waste oil at concentrations ranging between 10 and 100 mg/L was continuously applied to glass bead columns that resembled porous media. The majority of the alkylbenzenes and polynuclear aromatic compounds identified in the oil mixture were simultaneously biotransformed by aerobic biofilms within a 2-hour hydraulic residence time. In a methanogenic biofilm column with 2-day hydraulic residence time, the mixture of hydrocarbons was initially removed by sorption with complete breakthrough occurring after 300 days of operation. After 600 days, the methanogenic biofilm acclimated to several of the specific hydrocarbon components, and effluent concentrations decreased due to apparent biotransformation. Radiotracer studies with toluene, naphthalene, and 2-methyl-naphthalene indicated partial mineralization to CO2 under both aerobic and methanogenic conditions. Reaction rates observed in the laboratory biofilms indicate that biotransformation could be an effective process to control hydrocarbon contamination in the environment.
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Kotova, V. E., Yu А. Andreev, О. А. Mikhaylenko, and I. А. Ryazantseva. "ASSESSMENT OF PETROLEUM COMPONENT CONTAMINATION OF WATER IN THE TEMERNIK RIVER AND ITS INFLUENCE ON THE DON RIVER." Ecology. Economy. Informatics.System analysis and mathematical modeling of ecological and economic systems 1, no. 6 (2021): 112–17. http://dx.doi.org/10.23885/2500-395x-2021-1-6-112-117.

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Here, we report the results of petroleum component contamination assessment of the Temernik river and the Don river. Our aim was to study the hydrocarbon group content of petroleum components in the river water. Thus, we determined the mass concentrations of chemical oxygen demand, petroleum components, and aliphatic and polycyclic aromatic hydrocarbons. In the Temernik river, the concentrations of chemical oxygen demand, petroleum components, sum of aliphatic and polycyclic aromatic hydrocarbons were 21.4–34.4 mg/L, 0.14–6.0 mg/L, 10–18 μg/L, and 0.17–2.9 μg/L, respectively. The concentrations of chemical oxygen demand, petroleum components, and benzo[a]pyrene exceeded the maximum permissible concentration by 1.4–2.3, 2.8-120, and 1.3–5.8 times, respectively. In the Don river, the concentrations of chemical oxygen demand, petroleum components, sum of aliphatic and polycyclic aromatic hydrocarbons were 18.7-29.5 mg/L, 0.08- 0.16 mg/L, 8.2-12 μg/L, and 0.03-0.13 μg/L, respectively. The Severnoe reservoir was the less contaminated part of the river. The Temernik river estuary was the most contaminated part of the river. The pollutant concentrations increased in the Don River downstream of the Temernik river estuary. Therefore, the Temernik river influences on the Don river contamination. The chemical oxygen demand, petroleum components, and polycyclic aromatic hydrocarbons had the close distribution of concentrations in the rivers. However, the aliphatic hydrocarbon concentration changed in another way. The results of the study showed that the hydrocarbon groups of petroleum components can have different sources.
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Amine, H., J. Halwani, E. Gomez, and F. Merhabi. "Study of polycyclic aromatic hydrocarbon contamination in sediments and northern waters Lebanon: Rivers, transition zones and port sites." Lebanese Science Journal 19, no. 3 (December 27, 2018): 343–72. http://dx.doi.org/10.22453/lsj-019.3.343372.

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Contamination of the marine environment is associated with continental activities, marine activities and accidental spills. Providing answers to the origin of the contamination ofthe aquatic environment by PAHsis an objective of the present work. The originality of this work is to study pollutants in two types of matrix (water and sediments) during2 seasons (dry and wet) for an environmental monitoringof 6 years. This study provides a more complete overview of the state of contaminationin three coastal rivers, their transition zones and harborsin the Eastern Mediterranean following the Jiyyeh oil spill in 2006.Contaminantsconcentrations wereexpressed asthe sum of the 16 PAHs classified as prority substances by the United StatesEnvironmental Protection Agency(US-EPA). Overall,contamination was noted for all the analyzedsites. Oil spill seem to be one of the main sources of pollution in the area, as the highest levels of PAH(> 9000 ng.g-1s.w&> 8000 ng.L-1) were reported in the coastal environment of the three rivers and harbors.High levels of PAHswere also observed in rivers transitional zones, revealing the importance of terrestrial inputs (untreated wastewater discharge, leachate, solid wastes, etc.).Comparing the PAHsconcentrations measured in the sediments of the sitesanalyzed with the empirical sediment quality criteria "SQG" for the support of the general assessment of sediment toxicity, we can notice that some individual PAHand their sum can present a significant ecotoxicological risk to aquatic organisms. Further research through monitoring campaigns and toxicity tests is encouraged, as the exposure of the resident aquatic organisms and human population to these chemicals might be expected to increase over the years.
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Adams, Morgan, Ian Campbell, and Peter K. J. Robertson. "Novel Photocatalytic Reactor Development for Removal of Hydrocarbons from Water." International Journal of Photoenergy 2008 (2008): 1–7. http://dx.doi.org/10.1155/2008/674537.

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Hydrocarbons contamination of the marine environment generated by the offshore oil and gas industry is generated from a number of sources including oil contaminated drill cuttings and produced waters. The removal of hydrocarbons from both these sources is one of the most significant challenges facing this sector as it moves towards zero emissions. The application of a number of techniques which have been used to successfully destroy hydrocarbons in produced water and waste water effluents has previously been reported. This paper reports the application of semiconductor photocatalysis as a final polishing step for the removal of hydrocarbons from two waste effluent sources. Two reactor concepts were considered: a simple flat plate immobilised film unit, and a new rotating drum photocatalytic reactor. Both units proved to be effective in removing residual hydrocarbons from the effluent with the drum reactor reducing the hydrocarbon content by 90% under 10 minutes.
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Jahan, Sayka, and Vladimir Strezov. "Effect of shipping on the distribution of trace elements and petroleum hydrocarbons in the coastal basins of Australia: a review." Marine and Freshwater Research 71, no. 7 (2020): 794. http://dx.doi.org/10.1071/mf19031.

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This study surveyed levels of trace elements and hydrocarbon contamination in Australian seaport environments. Different activities contribute to discharges of trace elements and hydrocarbons into estuarine and marine environments. The elements of highest concern are As, Cu, Pb, Zn and Ni at Port Derwent, with Cd, Zn, Cu and Pb at Port Kembla and Zn and Cu at Botany Bay all exceeding the Australia and New Zealand Environment Conservation Council standards. Concentrations of some elements (Zn, Pb and Cu) in sediments at Port Kembla, Port Newcastle and the Derwent estuary are also higher than the Inter Sediment Quality Guidelines. The concentrations of polychlorinated biphenyls, dichlorodiphenyltrichloroethane and chlordane in the aquatic biota at two eastern ports (Port Jackson, Port Brisbane) are also among the highest in Australia and similar areas around the world. These findings provide baseline information on which to prioritise further research and formulate strategies to improve water quality in Australian port environments.
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Klein, Andrew G., Stephen T. Sweet, Terry L. Wade, José L. Sericano, and Mahlon C. Kennicutt. "Spatial patterns of total petroleum hydrocarbons in the terrestrial environment at McMurdo Station, Antarctica." Antarctic Science 24, no. 5 (July 4, 2012): 450–66. http://dx.doi.org/10.1017/s0954102012000429.

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AbstractFossil fuels are used throughout the United States Antarctic Program. Accidental releases of petroleum hydrocarbons are the leading source of environmental contamination. Since 1999 McMurdo Station has been the site of the most extensive environmental monitoring programme in Antarctica. Nearly 2500 surface soil samples were collected from 1999–2007 to determine the spatial “footprint” of petroleum hydrocarbons. Total petroleum hydrocarbons (TPH) concentrations were measured using a high-resolution capillary gas chromatographic method with flame ionization detection. Three distinct TPH patterns were detected: low molecular weight gasoline/JP5/AN8, residual weathered petroleum and an unresolved complex mixture of high molecular weight material. Overall TPH concentrations were low with 38% of the samples having TPH concentrations below 30 ppm and 58% below 100 ppm. Total petroleum hydrocarbon concentrations above 30 ppm are largely confined to the central portions of the station, along roads and in other areas where elevated TPH would be expected. Peripheral areas typically have TPH concentrations below 15 ppm. Areas of elevated TPH concentrations are patchy and of limited spatial extent, seldom extending over distances of 100 m. This environmental monitoring programme is ongoing and can serve as an example to other Antarctic programmes concerned with monitoring environmental impacts.
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., R. Arivarasan, and D. Sathishkumar . "Assessment of Hydrocarbon Oil Spill Pollutants in Agricultural Land." Asian Journal of Engineering and Applied Technology 7, no. 1 (March 5, 2018): 69–72. http://dx.doi.org/10.51983/ajeat-2018.7.1.858.

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Hydrocarbon contamination is a very serious problem whether it comes from petroleum, pesticides or other toxic organic matters. Oil spill have number of effects on the environment when petroleum products are released into the environment, they interact with the soil, groundwater, and microorganisms. Studies have mainly focused on identifying and characterizing these hydrocarbon degrading microbes, as well as how they scope with the soil/water/hydrocarbon interface, and how to improve this capacity. The main aim of the present study was to evaluate the biodegradation potential of microbes which was isolated from the hydrocarbon contaminated soil such as bacillus species and pseudomonas species. Those isolated microbes were inoculated into the contaminated soil, after 20 days of degradation process the physicochemical properties such as pH, temperature, color, GC-MS were analyzed and also prominent microbe was identified from the isolated microbes. Comparative study also done between aerated and non-aerated condition from that, aeration process enhanced the degradation rate.
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Green, Graham, and Peter D. Nichols. "Hydrocarbons and sterols in marine sediments and soils at Davis Station, Antarctica: a survey for human-derived contaminants." Antarctic Science 7, no. 2 (June 1995): 137–44. http://dx.doi.org/10.1017/s0954102095000198.

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A survey of hydrocarbons and sterols in marine and shoreline sediments was undertaken adjacent to Davis Station in Princess Elizabeth Land, Prydz Bay, Eastern Antarctica to determine the impact of a human settlement, including a sewage outfall on the local marine environment. Soil samples from selected locations onshore were also analysed to ascertain the extent of hydrocarbon contamination emanating from fuel storage facilities and other potential sources. The faecal sterol coprostanol was detected at 13.2 μg g−1 (60% of total sterols) in sediment adjacent to the Davis sewage outfall and up to 5.0 μg g−1 on the shoreline at Davis Beach. These concentrations indicate significant faecal contamination. The absence of coprostanol in faeces from the local wildlife confirms a human origin for this sewage biomarker. Hydrocarbons on the shoreline near Davis were present at up to 5.5 μg g−1 (dry weight of sediment). Biomarker profiles indicate an anthropogenic origin for these hydrocarbons. Onshore, degraded hydrocarbons derived from Special Antarctic Blend distillate were found at relatively high levels in soils at the fuel storage depot (up to 220 μg g−1). The source of these hydrocarbons appeared to be spillage from fuel storage tanks with possible contributions from fuel pipeline leakage and vehicle useage. Concentrations of polycyclic aromatic hydrocarbons in the soils were very low, generally below 1 ng g−1 (dry weight of sediment) for individual compounds.
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Arifin, Fazilah, Rozilawati Mohamed Razali, and Vigneswari Sevakumaran. "Isolation and Identification of Hydrocarbon-Degrading Bacteria from Polychaete Marphysa moribidii." Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology 16, no. 3 (December 17, 2021): 130–38. http://dx.doi.org/10.15578/squalen.567.

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Marine contamination caused by anthropogenic activities has side effects and causes severe contamination to the environment. Polychaetes are benthic organisms that live in the sediment and can be a good indicator of sediment contamination by organic compounds. In this study, bacterial strains were isolated and identified from the gut of polychaete worm Marphysa moribidii and the potential of the bacteria was evaluated to degrade hydrocarbon compounds. The isolated bacteria were primary and secondary screened on Minimal Salt Media (MSM) agar supplemented with 1% v/v of diesel oil. Diesel degradation analysis was performed by inoculating potential bacterium into MSM broth with 1% v/v diesel oil and incubated at 37 oC for 20 days. Diesel degradation percentage was analyzed using the gravimetric method, while the bacteria cell densities were measured using the standard plate count method. Then, the selected isolates were identified based on their morphological characteristics and 16S rDNA sequences. As a result, two bacteria isolates coded as Isolate 6 and Isolate 8 were able to degrade diesel oil up to 52.29% and 39.24% after 20 days of incubation. The 16S rDNA sequence analysis revealed that it was identified as Bacillus sp. strain UMTFA1 (RB) and Staphylococcus kloosii strain UMTFA2 (RS). Our result showed that these strains have the potential in oil-degrading processes, which will provide new insight into bioremediation process and decrease environmental pollution in soil and water contaminated with hydrocarbons.
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Ismail, Azimah, Hafizan Juahir, Saiful Bahri Mohamed, Mohd Ekhwan Toriman, Azlina Md Kassim, Sharifuddin Md Zain, Hadieh Monajemi, et al. "Support vector machines for oil classification link with polyaromatic hydrocarbon contamination in the environment." Water Science and Technology 83, no. 5 (February 1, 2021): 1039–54. http://dx.doi.org/10.2166/wst.2021.038.

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Abstract The main focus of this study is exploring the spatial distribution of polyaromatics hydrocarbon links between oil spills in the environment via Support Vector Machines based on Kernel-Radial Basis Function (RBF) approach for high precision classification of oil spill type from its sample fingerprinting in Peninsular Malaysia. The results show the highest concentrations of Σ Alkylated PAHs and Σ EPA PAHs in ΣTAH concentration in diesel from the oil samples PP3_liquid and GP6_Jetty achieving 100% classification output, corresponding to coherent decision boundary and projective subspace estimation. The high dimensional nature of this approach has led to the existence of a perfect separability of the oil type classification from four clustered oil type components; i.e diesel, bunker C, Mixture Oil (MO), lube oil and Waste Oil (WO) with the slack variables of ξ ≠ 0. Of the four clusters, only the SVs of two are correctly predicted, namely diesel and MO. The kernel-RBF approach provides efficient and reliable oil sample classification, enabling the oil classification to be optimally performed within a relatively short period of execution and a faster dataset classification where the slack variables ξ are non-zero.
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Abd‐Allah, Aly M. A., and Abd‐El‐Khalek El‐Sebae. "Hydrocarbon contamination of the Egyptian Mediterranean coast." Toxicological & Environmental Chemistry 47, no. 1-2 (January 1995): 15–22. http://dx.doi.org/10.1080/02772249509358122.

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Huntley, Sharon L., Lawrence J. Ritchie, Steven J. Setford, and Selwayan Saini. "The Development of a Sub-Surface Monitoring System for Organic Contamination in Soils and Groundwater." Scientific World JOURNAL 2 (2002): 1101–7. http://dx.doi.org/10.1100/tsw.2002.203.

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A major problem when dealing with environmental contamination is the early detection and subsequent surveillance of the contamination. This paper describes the potential of sub-surface sensor technology for the early detection of organic contaminants in contaminated soils, sediments, and landfill sites. Rugged, low-power hydrocarbon sensors have been developed, along with a data-logging system, for the early detection of phase hydrocarbons in soil. Through laboratory-based evaluation, the ability of this system to monitor organic contamination in water-based systems is being evaluated. When used in conjunction with specific immunoassays, this can provide a sensitive and low-cost solution for long-term monitoring and analysis, applicable to a wide range of field applications.
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Fryirs, Kirstie A., Erla G. Hafsteinsdóttir, Scott C. Stark, and Damian B. Gore. "Metal and petroleum hydrocarbon contamination at Wilkes Station, East Antarctica." Antarctic Science 27, no. 2 (September 10, 2014): 118–33. http://dx.doi.org/10.1017/s0954102014000443.

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AbstractThe management of sediment and water contamination from legacy waste is a significant problem in Antarctica. Although several reports have noted that there are contaminated sites at the abandoned Wilkes Station, a systematic attempt to assess the spatial scale of the problem has not been made, making development of clean-up or preservation programmes difficult. A contaminated site assessment for the old Wilkes Station and surrounds is presented in this paper. The Australian and New Zealand Environment and Conservation Council (ANZECC) sediment and water quality guidelines and background concentration levels (BCL) were used to assess the extent of contamination across Clark Peninsula. Of 67 sediment sites sampled, 72% were contaminated with at least one metal or metalloid, with values exceeding the ANZECC ISQG-High or 2 x BCL. Moreover, 19% were contaminated with four or more metals/metalloids. Of the 93 water samples collected, all but one was contaminated with at least one metal/metalloid concentration exceeding the guidelines, and 96% were contaminated with two or more metals/metalloids. For hydrocarbons in sediment and water, most samples were below quantitation limits. There is a complex pattern of contamination across Clark Peninsula that needs to be considered in future waste treatment, containment or removal operations, and for protection of heritage items.
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D’Ugo, Emilio, Milena Bruno, Arghya Mukherjee, Dhrubajyoti Chattopadhyay, Roberto Giuseppetti, Rita De Pace, and Fabio Magurano. "Characterization of microbial response to petroleum hydrocarbon contamination in a lacustrine ecosystem." Environmental Science and Pollution Research 28, no. 20 (April 19, 2021): 26187–96. http://dx.doi.org/10.1007/s11356-021-13885-8.

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AbstractMicrobiomes of freshwater basins intended for human use remain poorly studied, with very little known about the microbial response to in situ oil spills. Lake Pertusillo is an artificial freshwater reservoir in Basilicata, Italy, and serves as the primary source of drinking water for more than one and a half million people in the region. Notably, it is located in close proximity to one of the largest oil extraction plants in Europe. The lake suffered a major oil spill in 2017, where approximately 400 tons of crude oil spilled into the lake; importantly, the pollution event provided a rare opportunity to study how the lacustrine microbiome responds to petroleum hydrocarbon contamination. Water samples were collected from Lake Pertusillo 10 months prior to and 3 months after the accident. The presence of hydrocarbons was verified and the taxonomic and functional aspects of the lake microbiome were assessed. The analysis revealed specialized successional patterns of lake microbial communities that were potentially capable of degrading complex, recalcitrant hydrocarbons, including aromatic, chloroaromatic, nitroaromatic, and sulfur containing aromatic hydrocarbons. Our findings indicated that changes in the freshwater microbial community were associated with the oil pollution event, where microbial patterns identified in the lacustrine microbiome 3 months after the oil spill were representative of its hydrocarbonoclastic potential and may serve as effective proxies for lacustrine oil pollution.
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Hewelke, Edyta, and Dariusz Gozdowski. "Hydrophysical properties of sandy clay contaminated by petroleum hydrocarbon." Environmental Science and Pollution Research 27, no. 9 (January 10, 2020): 9697–706. http://dx.doi.org/10.1007/s11356-020-07627-5.

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AbstractThe aim of the presented research was to assess the changes in hydro-physical properties of sandy clay under the influence of petroleum hydrocarbon contamination. An understanding of these changes is fundamental in the right remedial actions and for further use of soil. Laboratory tests of inherently wettable sandy clay showed that the petroleum hydrocarbon induced potential soil water repellency (SWR) of extremely repellent class at the contamination of 18 g kg−1. The relationship between soil water potential (pF) and SWR determined by the WDPT test for given hydrocarbon contamination, i.e., 6, 12, 18, 30, 100 g kg−1, showed that the critical soil moisture value (CSMC) corresponds to the pF = 1.0 ÷ 1.5. Soil retention characteristic (pF) showed that an increase in hydrocarbon contamination from 0 to 100 g kg−1 caused a reduction of total available water for plants from about 0.19 to 0.06 cm cm−3. At the same time, in the pF = 1.5 ÷ 2.0 range, intensive soil pore drainage was observed. Statistically, significant effect of hydrocarbon contamination and soil moisture potential on SWR was found. Soil hydrophobicity limits the addition of soil retention, because a significant part of the precipitation can be transformed by surface runoff. The carried out tests showed that at a hydrocarbon contamination of 30 g kg−1, total rainfall amount 14 mm with an intensity of 2 mm h−1 was transformed into a surface drain in approx. 40%. The conducted studies demonstrate the adverse impact of hydrocarbon contamination on the soil’s hydro-physical properties. The soil water retention reduction and launching of the surface outflow, as a result of limiting the water penetration process resulting from SWR, change the agrohydrological conditions of the contaminated area. It can result as the imbalance of the flow of energy and matter in the ecosystem. The scenarios of environmental effects, among others, depend on the type of soil, the degree of its pollution, the type of ecosystem, and supporting activities undertaken by man. It should be taken into account that the increasing frequency of drought occurrence associated with climate change is conducive to the phenomenon of SWR regardless of the reasons for its occurrence.
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Mishra, Anuja, and Surya Pratap Singh. "AN APPROACH FOR THE BIODEGRADATION OF POLYCYCLIC AROMATIC HYDROCARBON." Journal of Experimental Biology and Agricultural Sciences 9, no. 1 (February 27, 2021): 65–74. http://dx.doi.org/10.18006/2021.9(1).65.74.

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Environmental pollution not only alters the environment but also changes the growth rate of various flora and fauna. Due to the irresponsible disposal of waste materials, the environment is going to be more and more polluted. Discharge of hydrocarbons in the water bodies is contaminating the water sources. These hydrocarbons are affecting the living organism. The solution to this problem has been found too expensive with little effects. To overcome this problem, some biological methods are introduced, in biological method; microbial degradation of hydrocarbons is the most promising method. The proposed study aimed to isolate and identify hydrocarbon-degrading bacterial species from the Mathura refinery, Mathura U.P. India. These species were isolated from petroleum-contaminated refinery water and grew on nutrient agar media, identified according to their distinct morphological, and biochemical characteristics, with16s rRNA sequencing. Results of this study confirmed the presence of various bacterial isolates such as Brevibacillus nitrificans, Algoriphagus shivajiensis, Bacillus marisflavi, Acinetobacter junii, Pseudomonas pseudoalcaligenes, and Bacillus pumilus from the collected samples based on the Bushnell Haas method and separation funnel method. Further, identified bacteria were tested for the maximum hydrocarbon degradation capacity in liquid culture, and results of the Gas Chromatography-Mass Spectrometry (GC-MS) suggested that only two bacterial species viz., P. pseudoalcaligenes and B. pumilus having the maximum hydrocarbon degradation capacity.
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Kharey, Gurpreet, Gabrielle Scheffer, and Lisa M. Gieg. "Combined Use of Diagnostic Fumarate Addition Metabolites and Genes Provides Evidence for Anaerobic Hydrocarbon Biodegradation in Contaminated Groundwater." Microorganisms 8, no. 10 (October 6, 2020): 1532. http://dx.doi.org/10.3390/microorganisms8101532.

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The widespread use of hydrocarbon-based fuels has led to the contamination of many natural environments due to accidental spills or leaks. While anaerobic microorganisms indigenous to many fuel-contaminated groundwater sites can play a role in site remediation (e.g., monitored natural attenuation, MNA) via hydrocarbon biodegradation, multiple lines of evidence in support of such bioremediation are required. In this study, we investigated two fuel-contaminated groundwater sites for their potential to be managed by MNA. Microbial community composition, biogeochemical indicators, fumarate addition metabolites, and genes diagnostic of both alkane and alkyl-monoaromatic hydrocarbon activation were assessed. Fumarate addition metabolites and catabolic genes were detected for both classes of hydrocarbon biodegradation at both sites, providing strong evidence for in situ anaerobic hydrocarbon biodegradation. However, relevant metabolites and genes did not consistently co-occur within all groundwater samples. Using newly designed mixtures of quantitative polymerase chain reaction (qPCR) primers to target diverse assA and bssA genes, we measured assA gene abundances ranging from 105–108 copies/L, and bssA gene abundances ranging from 105–1010 copies/L at the sites. Overall, this study demonstrates the value of investigating fuel-contaminated sites using both metabolites and genes diagnostic of anaerobic hydrocarbon biodegradation for different classes of hydrocarbons to help assess field sites for management by MNA.
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Amer, Ranya A., Francesca Mapelli, Hamada M. El Gendi, Marta Barbato, Doaa A. Goda, Anna Corsini, Lucia Cavalca, et al. "Bacterial Diversity and Bioremediation Potential of the Highly Contaminated Marine Sediments at El-Max District (Egypt, Mediterranean Sea)." BioMed Research International 2015 (2015): 1–17. http://dx.doi.org/10.1155/2015/981829.

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Coastal environments worldwide are threatened by the effects of pollution, a risk particularly high in semienclosed basins like the Mediterranean Sea that is poorly studied from bioremediation potential perspective especially in the Southern coast. Here, we investigated the physical, chemical, and microbiological features of hydrocarbon and heavy metals contaminated sediments collected at El-Max bay (Egypt). Molecular and statistical approaches assessing the structure of the sediment-dwelling bacterial communities showed correlations between the composition of bacterial assemblages and the associated environmental parameters. Fifty strains were isolated on mineral media supplemented by 1% crude oil and identified as a diverse range of hydrocarbon-degrading bacteria involved in different successional stages of biodegradation. We screened the collection for biotechnological potential studying biosurfactant production, biofilm formation, and the capability to utilize different hydrocarbons. Some strains were able to grow on multiple hydrocarbons as unique carbon source and presented biosurfactant-like activities and/or capacity to form biofilm and owned genes involved in different detoxification/degradation processes. El-Max sediments represent a promising reservoir of novel bacterial strains adapted to high hydrocarbon contamination loads. The potential of the strains for exploitation forin situintervention to combat pollution in coastal areas is discussed.
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Surya Ramadan, Bimastyaji, Agus Jatnika Effendi, and Qomarudin Helmy. "Integrating Electrokinetic and Bioremediation Process for Treating Oil Contaminated Low Permeability Soil." E3S Web of Conferences 31 (2018): 03005. http://dx.doi.org/10.1051/e3sconf/20183103005.

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Traditional oil mining activities always ignores environmental regulation which may cause contamination in soil and environment. Crude oil contamination in low-permeability soil complicates recovery process because it requires substantial energy for excavating and crushing the soil. Electrokinetic technology can be used as an alternative technology to treat contaminated soil and improve bioremediation process (biostimulation) through transfer of ions and nutrient that support microorganism growth. This study was conducted using a combination of electrokinetic and bioremediation processes. Result shows that the application of electrokinetic and bioremediation in low permeability soils can provide hydrocarbon removal efficiency up to 46,3% in 7 days operation. The highest amount of microorganism can be found in 3-days operation, which is 2x108 CFU/ml using surfactant as flushing fluid for solubilizing hydrocarbon molecules. Enhancing bioremediation using electrokinetic process is very potential to recover oil contaminated low permeability soil in the future.
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PÅLSSON, JONAS, and OLOF LINDÉN. "OIL CONTAMINATION IN THE NIGER DELTA." International Oil Spill Conference Proceedings 2014, no. 1 (May 1, 2014): 1706–18. http://dx.doi.org/10.7901/2169-3358-2014.1.1706.

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ABSTRACT Oil spills in the Niger Delta region of Nigeria has occurred frequently since oil extraction started in the 1950's. The oil spills originates from facilities and pipelines, leaks from ageing and abandoned infrastructure and from spills during transport and artisanal refining of stolen oil under primitive conditions. It is estimated that spills in Nigeria amount to 100 000 to 200 000 tons per year and have been doing so for almost 60 years. While a number of reports have been written about the Niger Delta and the civil unrest in this area during the last decades, very few scientific reports with actual data regarding the extent of the contamination has been published. This paper describes the contamination of sediments and water in a part of the Niger Delta, which has been particularly hard to assess for decades: Ogoniland. It does not discuss the origin of the oil spills. During 2010, the United Nations Environment Programme (UNEP) conducted an extensive environmental assessment of Ogoniland. The assessment was conducted at the request of the Nigerian government. During the assessment, drinking water samples were taken in wells and sediment and surface water samples were collected from streams, ponds and wetlands in and around Ogoniland from April to November. The levels found in the more contaminated sites are high enough to cause severe impacts on the ecosystem and human health. Extractable Petroleum Hydrocarbons (EPHs) reached levels of up to 7420 μg/l in surface water and drinking water wells show up to 42 200 μg/l. Benzene levels were measured up to 9000 μg/l, which is more than 900 times the WHO guidelines. EPH concentrations in sediments were up to 17 900 mg/kg. Polycyclic Aromatic Hydrocarbon (PAH) concentrations in sediments reached 8.0 mg/kg in the most contaminated sites. The impacts of this pollution were obvious to be seen, with large slicks of crude oil visible in the water and large areas of mangroves suffocated by oil. However, most sites did not show extremely high levels of EPH and PAH concentrations. Although the natural conditions for degradation of petroleum hydrocarbons are favorable with high temperatures and relatively high rainfall, the recovery of contaminated areas is prevented due to the chronic character of the contamination.
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Chicca, Ilaria, Simone Becarelli, and Simona Di Gregorio. "Microbial Involvement in the Bioremediation of Total Petroleum Hydrocarbon Polluted Soils: Challenges and Perspectives." Environments 9, no. 4 (April 15, 2022): 52. http://dx.doi.org/10.3390/environments9040052.

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Nowadays, soil contamination by total petroleum hydrocarbons is still one of the most widespread forms of contamination. Intervention technologies are consolidated; however, full-scale interventions turn out to be not sustainable. Sustainability is essential not only in terms of costs, but also in terms of restoration of the soil resilience. Bioremediation has the possibility to fill the gap of sustainability with proper knowledge. Bioremediation should be optimized by the exploitation of the recent “omic” approaches to the study of hydrocarburoclastic microbiomes. To reach the goal, an extensive and deep knowledge in the study of bacterial and fungal degradative pathways, their interactions within microbiomes and of microbiomes with the soil matrix has to be gained. “Omic” approaches permits to study both the culturable and the unculturable soil microbial communities active in degradation processes, offering the instruments to identify the key organisms responsible for soil contaminant depletion and restoration of soil resilience. Tools for the investigation of both microbial communities, their degradation pathways and their interaction, will be discussed, describing the dedicated genomic and metagenomic approaches, as well as the interpretative tools of the deriving data, that are exploitable for both optimizing bio-based approaches for the treatment of total petroleum hydrocarbon contaminated soils and for the correct scaling up of the technologies at the industrial scale.
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Filipovych, Volodymyr, Anton Mychak, Alexandr Kudryashov, and Ruslan Shevchuk. "Remote monitoring of the state of hydrocarbon pollution of the geological environment and assessment of the risks of dangerous situations in areas of long-term oil and gas production and compact living of the population." Ukrainian journal of remote sensing, no. 26 (September 19, 2020): 14–26. http://dx.doi.org/10.36023/ujrs.2020.26.179.

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The results of the analysis of geoecological problems of territories of long-term hydrocarbon production are presented. Based on the analysis of ground-based and remote studies, the possibilities of space data were determined during the eco-monitoring of hydrocarbon deposits. A methodological and technological scheme for assessing the risks of harming the environment is proposed. The list of tasks that can be solved using Earth remote sensing data:- control of environmental pollution by hydrocarbons (oil, gas) at different stages of the functioning of the oil and gas complex - from the search and exploitation of deposits, to the transportation, storage and processing of petroleum products;- flooding and flooding of territories of oil production by underground waters;- identification, mapping, field studies, discontinuous violations of various ranks, are ways of migration of oil and gas in the upper layers of the earth's crust;- identification and mapping of technogenic sources of gas contamination of the surface layer of the atmosphere, namely:- non-geometrical wells, oil and gas pipelines;- buried under modern sediments, pit-well houses, mines.Satellite monitoring consists of 4 stages. At the first, preliminary stage, objects of remote monitoring are determined, a base of satellite and thematic data is formed, the foundations of a future thematic GIS are laid. At the second stage, the actual detection (decryption) of objects and territories polluted with hydrocarbons is carried out. At the next, third stage, a set of field work is carried out in order to validate the research methodology and verify the data obtained using ground-based measurements.At the fourth, final stage, based on an analysis of all the information received, an assessment of the risks of dangerous situations is carried out and their possible consequences are predicted.The possibilities of assessing the risks of dangerous situations in areas of long-term hydrocarbon production according to remote sensing data are considered. A review of hazardous situations resulting from hydrocarbon production in the city of Borislav is given .; recommendations are proposed to reduce the risk of their occurrence.
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Patel, Khageshwar Singh, Shobhana Ramteke, Yogita Naik, Bharat Lal Sahu, Saroj Sharma, Jutta Lintelmann, and Matuschek Georg. "Contamination of Environment with Polycyclic Aromatic Hydrocarbons in India." Journal of Environmental Protection 06, no. 11 (2015): 1268–78. http://dx.doi.org/10.4236/jep.2015.611111.

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Suvorova, Iryna, Oleh Kravchenko, Vitalii Goman, and Ihor Baranov. "Criteria for Assessing the Energy-Ecological Effectiveness of using the Sludge of Waste Treatment Plants as Components of Liquid Composite Fuels." European Journal of Sustainable Development 9, no. 4 (October 1, 2020): 328. http://dx.doi.org/10.14207/ejsd.2020.v9n4p328.

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The challenging issues in the sustainable development of a state currently are the problems of protecting the environment from contamination, increasing the capacity of systems for recycling and reusing water, and the development of resource saving and non-waste technologies. The authors have developed and introduced the technology of hydrocavitation activation of liquid hydrocarbons to create composite fuels that can contain off-spec hydrocarbons, including stale fuel oil, oil tanker outwashes, stillage bottoms, coal, bio mass, industrial wastes and the sludge of wastewater treatment plants. The main criteria were defined for assessing the energy-ecological effectiveness of using sludge in municipal wastewater as a component of composite fuels. The criteria are as follows: the calorific capacity of the fuel mixture, the energy effectiveness of its use as an energy resource, consumer properties, and the environmental performance of combusting this kind of fuel. Using these criteria during the production and combustion of composite fuels obtained with hydrocavitation activation allows producing high-quality fuel mixtures with specified thermophysical and consumer properties. This opens the prospects of more effective usage of hydrocarbon resources and of solving simultaneously two problems: the production of surplus energy and environmental load reduction during the disposal and neutralisation of moisture-containing waste. Keywords: sludge; hydrocarbon resources; hydrocavitation activation; composite fuels; waste disposal and neutralisation
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Cripps, G. C., and J. Priddle. "Hydrocarbons in the Antarctic marine environment." Antarctic Science 3, no. 3 (September 1991): 233–50. http://dx.doi.org/10.1017/s0954102091000299.

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The Antarctic marine ecosystem contains low concentrations of a range of hydrocarbons, overwhelmingly biogenic in origin. The low natural levels of hydrocarbons and the small amount of local human activity make the Antarctic marine ecosystem suitable as a yardstick against which future global pollution by hydrocarbons can be assessed. At present, contamination is very low and difficult to resolve against the background. Local pollution is restricted to a very few events. A systematic programme with a clear definition of the natural background is proposed to monitor both global and local pollution.
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Cripps, G. C. "The extent of hydrocarbon contamination in the marine environment from a research station in the Antarctic." Marine Pollution Bulletin 25, no. 9-12 (January 1992): 288–92. http://dx.doi.org/10.1016/0025-326x(92)90684-x.

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Sui, Xin, Xuemei Wang, Yuhuan Li, and Hongbing Ji. "Remediation of Petroleum-Contaminated Soils with Microbial and Microbial Combined Methods: Advances, Mechanisms, and Challenges." Sustainability 13, no. 16 (August 18, 2021): 9267. http://dx.doi.org/10.3390/su13169267.

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The petroleum industry’s development has been supported by the demand for petroleum and its by-products. During extraction and transportation, however, oil will leak into the soil, destroying the structure and quality of the soil and even harming the health of plants and humans. Scientists are researching and developing remediation techniques to repair and re-control the afflicted environment due to the health risks and social implications of petroleum hydrocarbon contamination. Remediation of soil contamination produced by petroleum hydrocarbons, on the other hand, is a difficult and time-consuming job. Microbial remediation is a focus for soil remediation because of its convenience of use, lack of secondary contamination, and low cost. This review lists the types and capacities of microorganisms that have been investigated to degrade petroleum hydrocarbons. However, investigations have revealed that a single microbial remediation faces difficulties, such as inconsistent remediation effects and substantial environmental consequences. It is necessary to understand the composition and source of pollutants, the metabolic genes and pathways of microbial degradation of petroleum pollutants, and the internal and external aspects that influence remediation in order to select the optimal remediation treatment strategy. This review compares the degradation abilities of microbial–physical, chemical, and other combination remediation methods, and highlights the degradation capabilities and processes of the greatest microbe-biochar, microbe–nutrition, and microbe–plant technologies. This helps in evaluating and forecasting the chemical behavior of contaminants with both short- and long-term consequences. Although there are integrated remediation strategies for the removal of petroleum hydrocarbons, practical remediation remains difficult. The sources and quantities of petroleum pollutants, as well as their impacts on soil, plants, and humans, are discussed in this article. Following that, the focus shifted to the microbiological technique of degrading petroleum pollutants and the mechanism of the combined microbial method. Finally, the limitations of existing integrated microbiological techniques are highlighted.
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45

Walter, Juline M., Andrea Bagi, and Daniela M. Pampanin. "Insights into the Potential of the Atlantic Cod Gut Microbiome as Biomarker of Oil Contamination in the Marine Environment." Microorganisms 7, no. 7 (July 22, 2019): 209. http://dx.doi.org/10.3390/microorganisms7070209.

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Background: Microorganisms are widespread in all environments, including in and on animal bodies. The gut microbiome has an essential influence on fish health, and is affected by several persistent and harmful organic and inorganic contaminants. Considering the shifts in gut microbiota composition observed in those studies, we hypothesized that certain microbial groups in the gut can serve as indicators of pollution. To test this hypothesis, we explored the possibility of identifying key microbial players that indicate environmental contamination. Methods: Published 16S rRNA gene amplicon sequencing data generated from the gut microbiota of Atlantic cod caught in geographically different Norwegian waters were used for bacterial diversity comparison. Results: Different microbiomes were identified between the northern Norway and southern Norway samples. Several bacterial genera previously identified as polycyclic aromatic hydrocarbon degraders were present only in the samples collected in the southern Norway area, suggesting fish contamination with oil-related compounds. Conclusions: The results contribute to the identification of bacterial taxa present in the Atlantic cod gut that indicate fish exposure to contaminants in the marine environment.
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46

Mouallem, Carlos, Wilson Trigueiro de Sousa, Ivo Eyer Cabral, and Adilson Curi. "Perspectives for use of hydraulic fracturing in oil and gas production." Rem: Revista Escola de Minas 67, no. 4 (December 2014): 373–78. http://dx.doi.org/10.1590/0370-44672014670168.

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Hydraulic fracturing emerges currently, all over the world, as one of the more strategic techniques used by companies in the oil exploitation sector. This technique is characterized by its high productivity and profit in relation to conventional methods of hydrocarbon exploitation. However, in many countries, as is the case of Brazil, there are several divergences considering the employment of this methodology. Many renowned researchers attest that there are several irreversible environmental impacts generated by the use of this methodology. Among the main environmental impacts are the risk of groundwater level contamination, the risk of surface subsidence, and the risk of the environment contamination with fluids used in the process of the oil and gas extraction.
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47

Stefanakis, Alexandros I. "The Fate of MTBE and BTEX in Constructed Wetlands." Applied Sciences 10, no. 1 (December 23, 2019): 127. http://dx.doi.org/10.3390/app10010127.

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Hydrocarbon contamination of water resources is a global issue. These compounds are generated and discharged into the environment in industrial areas from chemical and petrochemical plants, oil refineries, power plants, and so forth. Fuel hydrocarbons, namely, BTEX (benzene, toluene, ethylbenzene, and xylenes) and MTBE (methyl tert-butyl ether), are commonly found in groundwater, posing environmental and health risks to humans and ecosystems. Nature-based technologies represent an alternative solution, providing high efficiency, an environmentally friendly character, simple operation, and cost efficiency, which are characteristics particularly desired by the international petroleum industry. This article discusses the use of the green technology of constructed wetlands to remediate water polluted with hydrocarbons. Although the number of related international experiences and studies is limited, the article presents the latest developments of wetland technology for the removal of MTBE and benzene-BTEX. The discussion includes the overall efficiency of the different wetland types that have been tested and used, the main transformation and removal processes that regulate the fate of BTEX and MTBE in constructed wetlands, and the potential for future investigations.
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48

Rusakov, Nikolay V., M. A. Vodyanova, N. Yu Starodubova, and L. G. Donerian. "METHODOLOGICAL AND CONCEPTUAL PROBLEMS OF OIL POLLUTION IN SOIL." Hygiene and sanitation 96, no. 10 (March 27, 2019): 929–33. http://dx.doi.org/10.18821/0016-9900-2017-96-10-929-933.

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The relevance of the normalization of petroleum hydrocarbons in soil is due, inter alia, to their ubiquity. Negative impacts on soil cover, atmospheric air, surface and groundwaters, ecological systems and public health are noted at all stages of development of oil fields - from drilling to industrial processing, liquidation of equipment and delivery to the consumer. In addition, various technologies for the destruction of oil contamination (re-cultivation, sanitation, etc.) are actively being developed, but they are not widely used, because there is no norm for a safe level of their content for humans and environmental objects. The article deals with problems of hygienic regulation of petroleum hydrocarbons in the soil. Methods are presented for the quantitative determination of oil and oil products in soil, as well as conceptual issues in the system for estimating oil as a soil pollutant. The subordinate legislation, taking into account the lists of pollutants, in respect of which state regulation measures in the field of environmental protection are applied, are sanctified. Toxic oxygen-containing products are shown to be formed asa result of the transformation of the hydrocarbon component of the component composition of the oil. Threshold concentrations of the safe content of petroleum hydrocarbons in the soil in a vegetation experiment (500 mg/kg), water migration - 10,000 mg/kg and a total of 21,000 mg/kg were determined. Priority directions of research on the establishment of a safe level of oil hydrocarbon contamination in the soil according to the airborne migration index of harmfulness, as well as the quantitative determination of petroleum hydrocarbons in agricultural plants are indicated.
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Arar, Sharif, Afnan Al-Hunaiti, Mohanad H. Masad, Androniki Maragkidou, Darren Wraith, and Tareq Hussein. "Elemental Contamination in Indoor Floor Dust and Its Correlation with PAHs, Fungi, and Gram+/− Bacteria." International Journal of Environmental Research and Public Health 16, no. 19 (September 23, 2019): 3552. http://dx.doi.org/10.3390/ijerph16193552.

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In this study, we performed elemental analysis for floor dust samples collected in Jordanian microenvironments (dwellings and educational building). We performed intercorrelation and cluster analysis between the elemental, polyaromatic hydrocarbon (PAH), and microorganism concentrations. In general, the educational building workshops had the highest elemental contamination. The age of the dwelling and its occupancy played a role on the elemental contamination level: older and more occupied dwellingshad greater contamination. The elemental contamination at a dwelling entrance was observed to be higher than in the living room. We found exceptionally high concentrations for Fe and Mn in the educational workshop and additionally, Hg, Cr, and Pb concentrations exceeded the limits set by the Canadian Council of Ministers of the Environment. According to the cluster analysis, we found three major groups based on location and contamination. According to the enrichment factor (EF) assessment, Al, Co, Mn, Ti, and Ba had EF < 2 (i.e., minimal enrichment) whereas P, S, Pb, Sb, Mo, Zn, Hg, and Cu had EF > 40 (i.e., extremely enriched). In contrast, Ca and P were geogenically enriched. Furthermore, significant Spearman correlations indicated nine subgroups of elemental contamination combined with PAHs and microbes.
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50

Palmer, Terence A., Andrew G. Klein, Stephen T. Sweet, Paul A. Montagna, Larry J. Hyde, Terry L. Wade, and Jennifer Beseres Pollack. "Anthropogenic effects on the marine environment adjacent to Palmer Station, Antarctica." Antarctic Science 34, no. 1 (December 7, 2021): 79–96. http://dx.doi.org/10.1017/s0954102021000535.

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AbstractLocalized contamination from research-related activities and its effects on macrofauna communities in the marine environment were investigated at Palmer Station, a medium-sized Antarctic research station. Relatively low concentrations of polycyclic aromatic hydrocarbons (PAHs; 32–302 ng g-1) and total petroleum hydrocarbons (TPHs; 0.9–8.9 μg g-1) were detected in sediments adjacent to the sewage outfall and pier, where most human activities were expected to have occurred, and at even lower concentrations at two seemingly reference areas (PAHs 6–30 ng g-1, TPHs 0.03–5.1 μg g-1). Elevated concentrations of PAHs in one sample taken in one reference area (816 ng g-1) and polychlorinated biphenyls (353 ng g-1) and dichloro-diphenyl-trichloroethane (3.2 and 25.3 ng g-1) in two samples taken adjacent to the sewage outfall indicate spatial heterogeneity of localized sediment contamination. Limpet (Nacella concinna) tissues collected adjacent to Palmer Station had high concentrations of PAHs, copper, lead, zinc and several other metals relative to outlying islands. Sediment and limpet tissue contaminant concentrations have decreased since the early 1990s following the Bahía Paraíso spill. Natural sediment characteristics affected macrofaunal community composition more than contamination adjacent to Palmer Station, presumably because of the low overall contamination levels.
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