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1

Sayuti, Irda. "Effect of Agitation to Hydrocarbon Degradation by a Hydrocarbonoclastic Bacterium isolated from Chevron Pacific Indonesia’s Waste Tank in Petapahan, Riau." International Journal of Ecophysiology 1, no. 2 (November 10, 2019): 146–50. http://dx.doi.org/10.32734/ijoep.v1i2.2825.

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Oxygen content is a limiting factor in the process of hydrocarbon compounds degradation by hydrocarbonoclastic bacteria. Oxygen may be supplied through agitation (stirring) during fermentation process by hydrocarbonoclastic bacteria. This study aims to to determine the optimal agitation speed for batch fermentation process by hydrocarbonoclastic bacteria isolated from the waste tank of PT Chevron Pacific Indonesia (CPI) Petapahan, Riau. This study was conducted in Biota laboratory, Universitas Andalas, West Sumatra, Indonesia. Hydrocarbonoclastic bacteria were recovered from waste samples by culturing into nutrient broth. Three different agitation speed viz. 110, 120, and 130 rpm were selected as optimization factors. The results show that the percentage of total petroleum hydrocarbon (TPH) degradation are 79.72, 87.49, and 88.35 for 110, 120, and 130 rpm, respectively. Meanwhile, chemical oxygen demand (COD) monitored during fermentation are 88.48, 90.06, and 90.16%, respectively. The agitation speed of 130 rpm is then designated as optimum factor for hydrocarbon degradation by hydrocarbonoclastic bacteria.
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2

Dewi, Ariyanti Suhita. "Application of docking method to assess the activity of hydrocarbonoclastic bacteria (HCB) from marine origin in bioremediation process." Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology 6, no. 2 (August 1, 2010): 45. http://dx.doi.org/10.15578/squalen.v6i2.60.

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Hydrocarbons are one of the main sources of acute and chronic stressors in marine environmentthat are potentially damaging the ecosystem if not properly overcame. As an attempt to restore theenvironment, microbial degradation is a logical solution owing to its low cost and environmentalfriendliness. Screening of microbes with bioremediation activities can be performed in vitrobymeans of high throughput screening (HTS) and/or in silicovia docking method. The latter haspractical advantages over the first in terms of time and cost. In this review, the use of virtualscreening is demonstrated to analyse the specificity of cytochrome-c peroxidase (CCP) enzymefrom hydrocarbonoclastic bacteria Marinobacter hydrocarbonoclasticus. Result showed thatCCP is a decent receptor for simple aromatic hydrocarbons. Despite previous reports on thealkane degradation activities of M. hydrocarbonoclasticus, this result demonstrates a newperspective on its potential to bioremediate low molecular weight polycyclic aromatic hydrocarbon(PAH) with moderate activity.
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3

Darmayati, Yeti, Shigeaki Harayama, Atsushi Yamazoe, Ariani Hatmanti, Sulistiani, Ruyitno Nuchsin, and Djoko Hadi Kunarso. "HYDROCARBONOCLASTIC BACTERIA FROM JAKARTA BAY AND SERIBU ISLANDS." Marine Research in Indonesia 33, no. 1 (June 30, 2008): 55–64. http://dx.doi.org/10.14203/mri.v33i1.506.

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Jakarta Bay has been known as one of the most polluted marine environment in Indonesia, with no exception by oil. Seribu Islands waters, located in the north of Jakarta Bay may have been impacted by this polluted condition.It’s sometimes also hit by oil spillage from pipe leakage. The purpose of this study is to isolate and identify hydrocarbonoclastic bacteria (oil and Polyaromatic Hydrocarbon degrading bacteria) from Jakarta Bay and Seribu Island waters. The bacteria were isolated from water and sediment/sand. Isolation was prepared by enriched samples in SWP medium with Arabian Light Crude Oil (ALCO). Screening for PAH degrading bacteria has been completed by using sublimation plate method in ONR7a medium and screening for oil degrading bacteria were conducted by using oil plated method with the same medium. Bacteria identifications were done based on l6sRNA gene. The results were analyzed using BLAST and showed that 131 potential hydrocarbonoclastic bacteria have been isolated from Jakarta Bay and Seribu Island waters. Most of them were oil degrading bacteria (41.98%) and the rest were PAH degrading bacteria. Oil pollution level may impact the number of strain of hydrocarbonoclastic bacteria isolated. Among the hydrocarbonoclastic bacteria isolated from Jakarta Bay and Seribu Islands, Alcanivorax, Marinobacter, Achromobacter and Bacillus were common hydrocarbonoclastic genera in Jakarta Bay and its surrounding waters. Alcanivorax spp. is important oil and PAH-degrader found not only in temperate waters, but in tropical waters as well.
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4

Nurhariyati, Tri, Ni’matuzahroh Ni’matuzahroh, and Tini Surtiningsih. "KEANEKARAGAMAN KHAMIR PENDEGRADASI MINYAK HASIL ISOLASI DARI PELABUHAN TANJUNG PERAK SURABAYA." Berkala Penelitian Hayati 9, no. 2 (June 30, 2004): 87–91. http://dx.doi.org/10.23869/bphjbr.9.2.20045.

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The aims of this research was to obtain diversity of hydrocarbonoclastic yeast isolated from Tanjung perak harbor Surabaya. Exploration of yeast was conducted by isolation and identification of isolated yeast. Identification of yeast based on characteristics of colonies, cell shape, and biochemical tests. From this research, it was obtained 9 hydrocarbonoclastic yeasts. They were 8 generas: Rhodotorula, Candida, Geotrichum, Torulopsis, Trichosporon, Cryptococcus, Debaryomyces, and Saccharomyces.
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5

Al-Mailem, D. M., M. K. Kansour, and S. S. Radwan. "Hydrocarbonoclastic biofilms based on sewage microorganisms and their application in hydrocarbon removal in liquid wastes." Canadian Journal of Microbiology 60, no. 7 (July 2014): 477–86. http://dx.doi.org/10.1139/cjm-2014-0214.

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Attempts to establish hydrocarbonoclastic biofilms that could be applied in waste-hydrocarbon removal are still very rare. In this work, biofilms containing hydrocarbonoclastic bacteria were successfully established on glass slides by submerging them in oil-free and oil-containing sewage effluent for 1 month. Culture-dependent analysis of hydrocarbonoclastic bacterial communities in the biofilms revealed the occurrence of the genera Pseudomonas, Microvirga, Stenotrophomonas, Mycobacterium, Bosea, and Ancylobacter. Biofilms established in oil-containing effluent contained more hydrocarbonoclastic bacteria than those established in oil-free effluent, and both biofilms had dramatically different bacterial composition. Culture-independent analysis of the bacterial flora revealed a bacterial community structure totally different from that determined by the culture-dependent method. In microcosm experiments, these biofilms, when used as inocula, removed between 20% and 65% crude oil, n-hexadecane, and phenanthrene from the surrounding effluent in 2 weeks, depending on the biofilm type, the hydrocarbon identity, and the culture conditions. More of the hydrocarbons were removed by biofilms established in oil-containing effluent than by those established in oil-free effluent, and by cultures incubated in the light than by those incubated in the dark. Meanwhile, the bacterial numbers and diversities were enhanced in the biofilms that had been previously used in hydrocarbon bioremediation. These novel findings pave a new way for biofilm-based hydrocarbon bioremediation, both in sewage effluent and in other liquid wastes.
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6

Gofar, Nuni. "Characterization of Petroleum Hydrocarbon Decomposing Fungi Isolated from Mangrove Rhizosphere." Journal of Tropical Soils 16, no. 1 (July 1, 2013): 39–45. http://dx.doi.org/10.5400/jts.2011.v16i1.39-45.

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The research was done to obtain the isolates of soil borne fungi isolated from mangrove rhizosphere which were capable of degrading petroleum hydrocarbon compounds. The soil samples were collected from South Sumatra mangrove forest which was contaminated by petroleum. The isolates obtained were selected based on their ability to survive, to grow and to degrade polycyclic aromatic hydrocarbons in medium containing petroleum residue. There were 3 isolates of soil borne hydrocarbonoclastic fungi which were able to degrade petroleum in vitro. The 3 isolates were identified as Aspergillus fumigates, A. parasiticus, and Chrysonilia sitophila. C. sitophila was the best isolate to decrease total petroleum hydrocarbon (TPH) from medium containing 5-20% petroleum residue.Keywords: Hydrocarbonoclastic fungi, hydrocarbon compounds, mangrove rhizosphere
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7

Valencia-Agami, Sonia S., Daniel Cerqueda-García, Sébastien Putzeys, María Magdalena Uribe-Flores, Norberto Ulises García-Cruz, Daniel Pech, Jorge Herrera-Silveira, M. Leopoldina Aguirre-Macedo, and José Q. García-Maldonado. "Changes in the Bacterioplankton Community Structure from Southern Gulf of Mexico During a Simulated Crude Oil Spill at Mesocosm Scale." Microorganisms 7, no. 10 (October 11, 2019): 441. http://dx.doi.org/10.3390/microorganisms7100441.

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The southern Gulf of Mexico (sGoM) is highly susceptible to receiving environmental impacts due to the recent increase in oil-related activities. In this study, we assessed the changes in the bacterioplankton community structure caused by a simulated oil spill at mesocosms scale. The 16S rRNA gene sequencing analysis indicated that the initial bacterial community was mainly represented by Gamma-proteobacteria, Alpha-proteobacteria, Flavobacteriia, and Cyanobacteria. The hydrocarbon degradation activity, measured as the number of culturable hydrocarbonoclastic bacteria (CHB) and by the copy number of the alkB gene, was relatively low at the beginning of the experiment. However, after four days, the hydrocarbonoclastic activity reached its maximum values and was accompanied by increases in the relative abundance of the well-known hydrocarbonoclastic Alteromonas. At the end of the experiment, the diversity was restored to similar values as those observed in the initial time, although the community structure and composition were clearly different, where Marivita, Pseudohongiella, and Oleibacter were detected to have differential abundances on days eight–14. These changes were related with total nitrogen (p value = 0.030 and r2 = 0.22) and polycyclic aromatic hydrocarbons (p value = 0.048 and r2 = 0.25), according to PERMANOVA. The results of this study contribute to the understanding of the potential response of the bacterioplankton from sGoM to crude oil spills.
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8

Baruah, Reshita, Dipak Jyoti Kalita, Binoy K. Saikia, Arvind Gautam, Anil Kumar Singh, and Hari Prasanna Deka Boruah. "Native hydrocarbonoclastic bacteria and hydrocarbon mineralization processes." International Biodeterioration & Biodegradation 112 (August 2016): 18–30. http://dx.doi.org/10.1016/j.ibiod.2016.04.032.

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9

Doumenq, P., E. Aries, L. Asia, M. Acquaviva, J. Artaud, M. Gilewicz, G. Mille, and J. C. Bertrand. "Influence of n-alkanes and petroleum on fatty acid composition of a hydrocarbonoclastic bacterium: Marinobacter hydrocarbonoclasticus strain 617." Chemosphere 44, no. 4 (August 2001): 519–28. http://dx.doi.org/10.1016/s0045-6535(00)00521-x.

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10

Al-Wahaib, Dhuha, Dhia Al-Bader, Dana K. Al-Shaikh Abdou, Mohamed Eliyas, and Samir S. Radwan. "Consistent Occurrence of Hydrocarbonoclastic Marinobacter Strains in Various Cultures of Picocyanobacteria from the Arabian Gulf: Promising Associations for Biodegradation of Marine Oil Pollution." Journal of Molecular Microbiology and Biotechnology 26, no. 4 (2016): 261–68. http://dx.doi.org/10.1159/000445686.

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Fifteen nonaxenic cultures of picocyanobacteria were isolated from the Arabian Gulf, from which 122 heterotrophic bacterial strains were obtained. Based on their 16S rRNA gene sequences, those strains were affiliated with 22 different species, 82.8% of which belonged to the genus <i>Marinobacter</i>, known to comprise hydrocarbonoclastic strains. The remaining species belonged to the genera <i>Alcanivorax, Bacillus, Halomonas, Mesorhizobium, and Paenibacillus, </i>and a Bacteriodetes bacterium also known to comprise hydrocarbonoclastic strains. All the picocyanobacterial cultures harbored one or more strains of <i>Marinobacter</i>. <i>Marinobacter</i> in addition to <i>Alcanivorax</i> and other genera isolated from those picocyanobacteria grew on Tween 80, crude oil, and pure hydrocarbons as sole sources of carbon and energy, i.e. they are related to the obligate hydrocarbonoclastic bacteria group. They consumed crude oil, <i>n</i>-octadecane, and phenanthrene in batch cultures. The results indicated that <i>Marinobacter</i> isolates seemed to grow better and consume more oil in the presence of their host picocyanobacteria than in their absence. Such natural microbial associations assumingly play a role in bioremediation of spilled hydrocarbons in the Arabian Gulf. Similar associations probably occur in other marine environments as well and are active in oil spill removal.
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11

Coelho, FJRC, S. Sousa, L. Santos, AL Santos, A. Almeida, NCM Gomes, and Â. Cunha. "Exploring hydrocarbonoclastic bacterial ­communities in the estuarine surface microlayer." Aquatic Microbial Ecology 64, no. 2 (September 1, 2011): 185–95. http://dx.doi.org/10.3354/ame01526.

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12

Sevilla, Emma, Luis Yuste, and Fernando Rojo. "Marine hydrocarbonoclastic bacteria as whole-cell biosensors forn-alkanes." Microbial Biotechnology 8, no. 4 (April 15, 2015): 693–706. http://dx.doi.org/10.1111/1751-7915.12286.

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13

Goréguès, Christelle, Valérie Michotey, and Patricia Bonin. "Isolation of hydrocarbonoclastic denitrifying bacteria from berre microbial mats." Ophelia 58, no. 3 (December 2004): 263–70. http://dx.doi.org/10.1080/00785236.2004.10410234.

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14

Sivaraman, C., A. Ganguly, M. Nikolausz, and S. Mutnuri. "Isolation of hydrocarbonoclastic bacteria from bilge oil contaminated water." International Journal of Environmental Science & Technology 8, no. 3 (June 2011): 461–70. http://dx.doi.org/10.1007/bf03326232.

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15

Powell, James C., Patrick E. Dabinett, and John A. Gow. "An annual cycle of abundance and activity of heterotrophic bacteria and abundance of hydrocarbonoclastic bacteria in Newfoundland coastal water." Canadian Journal of Microbiology 33, no. 5 (May 1, 1987): 377–82. http://dx.doi.org/10.1139/m87-066.

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An annual cycle of abundance and activity was determined for bacteria in Newfoundland coastal water that is dominated by the Labrador Current. Both in abundance and activity, the population showed characteristics similar to those reported for bacteria in cold ocean environments. The number of bacteria per litre determined by the acridine orange direct count method ranged from an average low of 0.25 × 108 in winter to an average high of 2.8 × 108 in summer. Activity, determined by the kinetic method, was correlated with temperature. The average heterotrophic potential (Vmax) was 3 ng glutamate. L−1∙h−1 in winter and 54.7 ng glutamate. L−1∙h−1 in summer. The average turnover times were 2632 and 256 h, respectively, during the same periods. The population of hydrocarbonoclastic bacteria, estimated by a most probable number method, constituted 4.4% of the viable bacterial population estimated by the standard plate count method, although the former were most abundant near the water's surface. The number of hydrocarbonoclastic bacteria was correlated with temperature and with Vmax of the heterotrophic bacterial population.
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16

Sayuti, Irda, Yusni Siregar, Bintal Amin, Anthoni Agustien, and Akmal Djamaan. "Identification of Bacterial Hydrocarbonoclastic in Waste Tanks, Petapahan, Riau, Indonesia, using 16srRNA." Journal of Pure and Applied Microbiology 12, no. 2 (June 30, 2018): 671–77. http://dx.doi.org/10.22207/jpam.12.2.25.

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17

Devi, Sashi Prava, and Dhruva Kumar Jha. "Screening of Bacteria Isolated from Refinery Sludge of Assam for Hydrocarbonoclastic Activities." Journal of Pure and Applied Microbiology 14, no. 2 (June 25, 2020): 1453–65. http://dx.doi.org/10.22207/jpam.14.2.43.

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18

Gofar, Nuni. "Synergism of Wild Grass and Hydrocarbonoclastic Bacteria in Petroleum Biodegradation." JOURNAL OF TROPICAL SOILS 18, no. 2 (June 13, 2013): 161. http://dx.doi.org/10.5400/jts.2013.v18i2.161-168.

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The concept of plants and microbes utilization for remediation measure of pollutant contaminated soil is the newest development in term of petroleum waste management technique. The research objective was to obtain wild grass types and hydrocarbonoclastic bacteria which are capable to synergize in decreasing petroleum concentration within petroleum contaminated soil. This research was conducted by using randomized completely block design. This research was conducted by using randomized completely block design. The first factor treatments were consisted of without plant, Tridax procumbens grass and Lepironia mucronata grass. The second factor treatments were consisted of without bacterium, single bacterium of Alcaligenes faecalis, single bacterium of Pseudomonas alcaligenes, and mixed bacteria of Alcaligenes faecalis with P. alcaligenes. The results showed that mixed bacteria (A. faecalis and P. alcaligenes) were capable to increase the crown and roots dry weights of these two grasses, bacteria population, percentage of TPH (total petroleum hydrocarbon) decrease as well as TPH decrease and better pH value than that of single bacterium. The highest TPH decrease with magnitude of 70.1% was obtained on treatment of L. mucronata grass in combination with mixed bacteria.[How to Cite: Gofar N. 2013.Synergism of Wild Grass and Hydrocarbonoclastic Bacteria in Petroleum Biodegradation. J Trop Soils 18 (2): 161-168. Doi: 10.5400/jts.2013.18.2.161][Permalink/DOI: www.dx.doi.org/10.5400/jts.2013.18.2.161]REFERENCESBello YM. 2007. Biodegradation of Lagoma crude oil using pig dung. Afr J Biotechnol 6: 2821-2825.Gerhardt KE, XD Huang, BR Glick and BM Greenberg. 2009. Phytoremediation and rhizoremediation of organic soil contaminants: Potential and challenges. Plant Sci 176: 20-30.Glick BR. 2010. Using soil bacteria to facilitate phytoremediation. Biotechnol Adv 28: 367-374. Gofar N. 2011. Characterization of petroleum hydrocarbon decomposing fungi isolated from mangrove rhizosphere. J Trop Soils 16(1): 39-45. doi: 10.5400/jts.2011.16.1.39Gofar N. 2012. Aplikasi isolat bakteri hidrokarbonoklastik asal rhizosfer mangrove pada tanah tercemar minyak bumi. J Lahan Suboptimal 1: 123-129 (in Indonesian). Hong WF, IJ Farmayan, CY Dortch, SK Chiang and JL Schnoor. 2001. Environ Sci Technol 35: 1231.Khashayar T and T Mahsa. 2010. Biodegradation potential of petroleum hydrocarbons by bacterial diversity in soil. Morld App Sci J 8: 750-755.Lal B and S Khanna. 1996. Degradation of Crude Oil by Acinetobacter calcoaceticus and Alcaligenes odorans, J Appl Bacteriol 81: 355- 362.Mackova M, D Dowling and T Macek. 2006. Phytoremediation and rhizoremediation: Theoretical background. Springer, Dordrecht, Netherlands. 300 p. Malik ZA and S Ahmed. 2012. Degradation of petroleum hydrocarbons by oil field isolated bacterial consortium. Afr J Biotechnol 11: 650-658.Mendez MO and RM Maier. 2008. Phytostabilization of mine tailings in arid and semiarid environment an emerging remediation technology. Environ Health Prospect 116: 278-283.Milic JS, VP Beskoski, MV Ilic, SM Ali, GDJ Cvijovic and MM Vrvic. 2009. Bioremediation of soil heavily contaminated with crude oil and its products: composition of the microbial consortium. J Serb Chem Soc 74: 455-460.Mukre AM, AA Hamid, A Hamzah and WM Yusoff. 2008. Development of three bacteria consortium for the bioremediation of crude petroleum-oil in contaminated water. J Biol Sci 8: 73-79.Ndimele PE. 2010. A review on the phytioremediation of petroleum hydrocarbon. Pakistan J Biol Sci 12: 715-722.Newman LA and CM Reynolds. 2004. Phytoremediation of organic compounds. Curr Opin Biotechnol 15: 225-230.Onwuka F, N Nwachoko, and E Anosike. 2012. Determination of total petroleum hydrocarbon (TPH) and some cations (Na+, Ca2+ and Mg2+) in a crude oil polluted soil and possible phytoremediation by Cynodon dactylon L (Bermuda grass). J Environ Earth Sci 2: 12-17.Pezeshki SR, MW Hester, Q Lin and JA Nyman. 2000. The effect of oil spill and clean-up on dominant US Gulf Coast Marsh Macrophytes: a review. Environ Pollution 108: 129-139.Pikoli MR, P Aditiawati and DI Astuti. 2000. Isolasi bertahap dan identifikasi isolat bakteri termofilik pendegradasi minyak bumi dari sumur bangko. Laporan Penelitian pada Jurusan Biologi, ITB, Bandung (unpublished, in Indonesian).Pilon-Smits E and JL Freeman. 2006. Environmental cleanup using plants: biotechnological advances and ecological considerations. Front Ecol Environ 4: 203-10. Rahman KSM, JT Rahman, P Lakshmanaperumalsamy, and IM Banat. 2002. Towards efficient crude oil degradation by a mixed bacterial consortium. Bioresource Technol 85: 257-261.Rossiana N. 2004. Oily Sludge Bioremediation with Zeolite and Microorganism and It’s Test with Albizia Plant (Paraserianthes falcataria) L (Nielsen). Laboratory of Environmental Microbiology, Department of Biology Padjadjaran University, Bandung (unpublished).Rossiana, N. 2005. Penurunan Kandungan Logam Berat dan Pertumbuhan Tanaman Sengon (Paraserianthes falcataria L (Nielsen) Bermikoriza dalam Media Limbah Lumpur Minyak Hasil Ekstraksi. Laboratorium Mikrobiologi dan Biologi Lingkungan Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Padjajaran, Bandung (in Indonesian).Sathishkumar M, B Arthur Raj, B Sang-Ho, and Y Sei-Eok. 2008. Biodegradation of crude oil by individual bacterial strains and a mixed bacterial consortium isolated from hydrocarbon contaminated areas clean. Ind J Biotechnol 36: 92-96.Shirdam R, AD Zand, GN Bidhendi and N Mehrdadi. 2008. Phytoremediation of hydrocarbon-contaminated soils with emphasis on effect of petroleum hydrocarbons on the growth of plant species. Phytoprotection 89: 21-29.Singer AC, DE Crowley and IP Thompson. 2003. Secondary plant metabolites in phytoremediation and biotransformation. Trends Biotechnol 21: 123-130.Singh A and OP Ward. 2004. Applied Bioremediation and Phytoremediation. Springler, Berlin, 281p.Surtikanti H and W Surakusumah. 2004. Peranan Tanaman dalam Proses Bioremediasi Oli Bekas dalam Tanah Tercemar. Ekol Biodivers Trop 2: 48-52 (in Indonesian).Wenzel WW. 2009. Rhizosphere processes and management in plant-assisted bioremediation (phytoremediation) of soil. Plant Soil 321: 385-408.Widjajanti H, I Anas, N Gofar and MR Ridho. 2010. Screening of petroleum hydrocarbons degrading bacteria as a bioremediating agents from mangrove areas. Proceeding of International Seminar, workshop on integrated lowland development and management, pp. C7 1-9.Widjajanti H. 2012. Bioremediasi Minyak Bumi Menggunakan Bakteri dan Kapang Hidrokarbonoklastik dari Kawasan Mangrove Tercemar Minyak Bumi. [Disertasi]. Universitas Sriwijaya (in Indonesian).
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19

Etok, C., O. Akan, and A. Adegoke. "Bioremediation of Crude Oil Contaminated Soils Using Surfactants and Hydrocarbonoclastic Bacteria." British Microbiology Research Journal 9, no. 2 (January 10, 2015): 1–6. http://dx.doi.org/10.9734/bmrj/2015/6196.

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Thompson, Haydn, Angelina Angelova, Bernard Bowler, Martin Jones, and Tony Gutierrez. "Enhanced crude oil biodegradative potential of natural phytoplankton-associated hydrocarbonoclastic bacteria." Environmental Microbiology 19, no. 7 (July 2017): 2843–61. http://dx.doi.org/10.1111/1462-2920.13811.

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Bharali, Pranjal, Salam Pradeep Singh, Yasir Bashir, Nipu Dutta, Bolin Kumar Konwar, and Chingakham Brajakishor Singh. "Characterization and assessment of biosurfactant producing indigenous hydrocarbonoclastic bacteria: potential application in bioremediation." Nova Biotechnologica et Chimica 17, no. 2 (December 1, 2018): 103–14. http://dx.doi.org/10.2478/nbec-2018-0011.

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Abstract Petroleum and hydrocarbons contamination can be remediated by physical, chemical or biological methods. Among these, in situ bioremediation is considered to be environmentally friendly because it restores the soil structure, requires less energy input and involves the notable removal after degradation of biosurfactant. The present study involves the characterization and assessment of biosurfactant producing indigenous hydrocarbonoclastic bacteria and their potential application in bioremediation processes. Three bacterial strains were isolated from various crude oil contaminated environments and characterized using standard identification techniques. The results clearly demonstrate the capability of utilizing hydrocarbon and biosurfactant produced by the bacterial strains. 16S rDNA sequencing followed by BLAST analysis revealed their similarity to Pseudomonas aeruginosa. The physico-chemical characterization of the biosurfactants revealed significant surface properties with stability at extreme temperature conditions (up to 121˚C), pH (5 - 8) and salinity (up to 4 %). Further, the mass spectrometry confirmed predominance of di-rhamnolipids in biosurfactant mixtures. The biosurfactants were found to be efficient in the removal of crude oil from the contaminated sand suggesting its applicability in bioremediation technology. Further, improved discharge of crude oil at elevated temperatures also confirms their thermo-stability which, could be exploited in microbial enhanced oil recovery processes. Thus, the applications of biosurfactants produced by the indigenous hydrocarbonoclastic strains appeared to be advantageous for bioremediation of petroleum-contaminated environments.
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Ojeda-Morales, Marcia Eugenia, Miguel Ángel Hernández-Rivera, José Gabriel Martínez-Vázquez, Yolanda Córdova-Bautista, and Yuridia Evelin Hernández-Cardeño. "Optimal Parameters for in Vitro Development of the Fungus Hydrocarbonoclastic Penicillium sp." Advances in Chemical Engineering and Science 03, no. 04 (2013): 19–29. http://dx.doi.org/10.4236/aces.2013.34a1004.

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Hernández-Rivera, M. A., M. E. Ojeda-Morales, J. G. Martínez-Vázquez, V. M. Villegas-Cornelio, and Y. Córdova-Bautista. "OPTIMAL PARAMETERS FORT In Vitro DEVELOPMENT OF THE HYDROCARBONOCLASTIC MICROORGANISM Proteus sp." Journal of soil science and plant nutrition 11, no. 1 (2011): 29–43. http://dx.doi.org/10.4067/s0718-95162011000100003.

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Ganesh Kumar, A., N. Nivedha Rajan, R. Kirubagaran, and G. Dharani. "Biodegradation of crude oil using self-immobilized hydrocarbonoclastic deep sea bacterial consortium." Marine Pollution Bulletin 146 (September 2019): 741–50. http://dx.doi.org/10.1016/j.marpolbul.2019.07.006.

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25

Brito, Elcia Margareth S., Rémy Guyoneaud, Marisol Goñi-Urriza, Antony Ranchou-Peyruse, Arnaud Verbaere, Miriam A. C. Crapez, Julio César A. Wasserman, and Robert Duran. "Characterization of hydrocarbonoclastic bacterial communities from mangrove sediments in Guanabara Bay, Brazil." Research in Microbiology 157, no. 8 (October 2006): 752–62. http://dx.doi.org/10.1016/j.resmic.2006.03.005.

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Cocarta, D. M., D. M. Dumitru, L. Pesciaroli, M. Felli, B. Raduly, and S. Crognale. "Cultivable Hydrocarbonoclastic Microbial Community from Historically Polluted Soil: Tests for Consortium Development." Soil and Sediment Contamination: An International Journal 28, no. 3 (February 19, 2019): 334–45. http://dx.doi.org/10.1080/15320383.2019.1578335.

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Manilla-Pérez, Efraín, Alvin Brian Lange, Heinrich Luftmann, Horst Robenek, and Alexander Steinbüchel. "Neutral lipid production in Alcanivorax borkumensis SK2 and other marine hydrocarbonoclastic bacteria." European Journal of Lipid Science and Technology 113, no. 1 (November 17, 2010): 8–17. http://dx.doi.org/10.1002/ejlt.201000374.

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Stauffert, Magalie, Cristiana Cravo-Laureau, and Robert Duran. "Structure of hydrocarbonoclastic nitrate-reducing bacterial communities in bioturbated coastal marine sediments." FEMS Microbiology Ecology 89, no. 3 (June 23, 2014): 580–93. http://dx.doi.org/10.1111/1574-6941.12359.

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Rodrigues, Edmo Montes, Dionéia Evangelista Cesar, Renatta Santos de Oliveira, Tatiane de Paula Siqueira, and Marcos Rogério Tótola. "Hydrocarbonoclastic bacterial species growing on hexadecane: Implications for bioaugmentation in marine ecosystems." Environmental Pollution 267 (December 2020): 115579. http://dx.doi.org/10.1016/j.envpol.2020.115579.

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30

Kadali, Krishna K., Keryn L. Simons, Petra J. Sheppard, and Andrew S. Ball. "Mineralisation of Weathered Crude Oil by a Hydrocarbonoclastic Consortia in Marine Mesocosms." Water, Air, & Soil Pollution 223, no. 7 (May 12, 2012): 4283–95. http://dx.doi.org/10.1007/s11270-012-1191-8.

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Akiri-Obaroakpo, Tosan, Emmanuel Imarhiagbe, and Frederick Ekhaise. "Assessment of enhanced biodegradation potentials of animal wastes on diesel-contaminated soil." Studia Universitatis Babeş-Bolyai Biologia 65, no. 2 (December 20, 2020): 19–29. http://dx.doi.org/10.24193/subbbiol.2020.2.02.

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Oil spillage is a menace, crippling most oil-producing regions around the globe. The aim of this study was to access the role of poultry litter and cow dung in enhancing biodegradation of diesel-contaminated soil. The treatment sets were split into three levels of diesel pollution (50 mL, 100 mL and 150 mL) amended with poultry litters, cow dung and a mixture of both amendments. The microbiological properties-and the total petroleum hydrocarbon content was analyzed for a period of six months using the pour plate techniques and Gas Chromatography (GC-FID), respectively. Agarose gel electrophoresis was used for plasmid detection. Mean total heterotrophic bacterial counts ranged between 40.5±0.5 x104 cfu-1 and 102.0 ±4.0 x104 cfu-1, for C1 (soil with poultry litter and cow dung with 50mL diesel) and Control 2. The mean total hydrocarbonoclastic bacterial counts ranged from 42.0±2.0 x104 cfu-1 to 66.5±2.5 x104 cfu-1 for B1 (soil with cow dung with 50mL diesel) and C3 (soil with poultry litter and cow dung with 150mL diesel). Bacillus subtilis (25.7%) and Staphylococcus aureus (4.73%) were reported as the isolates with the highest and least percentage frequency of occurrence. The percentage of diesel oil degradation was highest in C1 (98.5%) and lowest in Control 1 (31.6%). Plasmid extraction studies carried revealed that two out of the five hydrocarbonoclastic bacteria had both plasmids and chromosomal genes. The result has indicated the enhanced capacity of mixed amendments relative to individual waste treatment used in this study and should be recommended for bioremediation application.
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Kalscheuer, Rainer, Tim Stöveken, Ursula Malkus, Rudolf Reichelt, Peter N. Golyshin, Julia S. Sabirova, Manuel Ferrer, Kenneth N. Timmis, and Alexander Steinbüchel. "Analysis of Storage Lipid Accumulation in Alcanivorax borkumensis: Evidence for Alternative Triacylglycerol Biosynthesis Routes in Bacteria." Journal of Bacteriology 189, no. 3 (November 22, 2006): 918–28. http://dx.doi.org/10.1128/jb.01292-06.

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ABSTRACT Marine hydrocarbonoclastic bacteria, like Alcanivorax borkumensis, play a globally important role in bioremediation of petroleum oil contamination in marine ecosystems. Accumulation of storage lipids, serving as endogenous carbon and energy sources during starvation periods, might be a potential adaptation mechanism for coping with nutrient limitation, which is a frequent stress factor challenging those bacteria in their natural marine habitats. Here we report on the analysis of storage lipid biosynthesis in A. borkumensis strain SK2. Triacylglycerols (TAGs) and wax esters (WEs), but not poly(hydroxyalkanoic acids), are the principal storage lipids present in this and other hydrocarbonoclastic bacterial species. Although so far assumed to be a characteristic restricted to gram-positive actinomycetes, substantial accumulation of TAGs corresponding to a fatty acid content of more than 23% of the cellular dry weight is the first characteristic of large-scale de novo TAG biosynthesis in a gram-negative bacterium. The acyltransferase AtfA1 (ABO_2742) exhibiting wax ester synthase/acyl-coenzyme A:diacylglycerol acyltransferase (WS/DGAT) activity plays a key role in both TAG and WE biosynthesis, whereas AtfA2 (ABO_1804) was dispensable for storage lipid formation. However, reduced but still substantial residual TAG levels in atfA1 and atfA2 knockout mutants compellingly indicate the existence of a yet unknown WS/DGAT-independent alternative TAG biosynthesis route. Storage lipids of A. borkumensis were enriched in saturated fatty acids and accumulated as insoluble intracytoplasmic inclusions exhibiting great structural variety. Storage lipid accumulation provided only a slight growth advantage during short-term starvation periods but was not required for maintaining viability and long-term persistence during extended starvation phases.
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Kadali, Krishna K., Keryn L. Simons, Pawel P. Skuza, Robert B. Moore, and Andrew S. Ball. "A complementary approach to identifying and assessing the remediation potential of hydrocarbonoclastic bacteria." Journal of Microbiological Methods 88, no. 3 (March 2012): 348–55. http://dx.doi.org/10.1016/j.mimet.2011.12.006.

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34

Rahul, K., Ch Sasikala, L. Tushar, R. Debadrita, and Ch V. Ramana. "Alcanivorax xenomutans sp. nov., a hydrocarbonoclastic bacterium isolated from a shrimp cultivation pond." International Journal of Systematic and Evolutionary Microbiology 64, Pt_10 (October 1, 2014): 3553–58. http://dx.doi.org/10.1099/ijs.0.061168-0.

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Two bacterial strains (JC109T and JC261) were isolated from a sediment sample collected from a shrimp cultivation pond in Tamil Nadu (India). Cells were Gram-stain-negative, motile rods. Both strains were positive for catalase and oxidase, hydrolysed Tween 80, and grew chemo-organoheterotrophically with an optimal pH of 6 (range pH 4–9) and at 30 °C (range 25–40 °C). Based on 16S rRNA gene sequence analysis, strains JC109T and JC261 were identified as belonging to the genus Alcanivorax with Alcanivorax dieselolei B-5T (sequence similarity values of 99.3 and 99.7 %, respectively) and Alcanivorax balearicus MACL04T (sequence similarity values of 98.8 and 99.2 %, respectively) as their closest phylogenetic neighbours. The 16S rRNA gene sequence similarity between strains JC109T and JC261 was 99.6 %. The level of DNA–DNA relatedness between the two strains was 88 %. Strain JC109T showed 31±1 and 26±2 % DNA–DNA relatedness with A. dieselolei DSM 16502T and A. balearicus DSM 23776T, respectively. The DNA G+C content of strains JC109T and JC261 was 54.5 and 53.4 mol%, respectively. Polar lipids of strain JC109T included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phospholipids and two unidentified lipids. The major fatty acids were C10 : 0, C12 : 0, C16 : 0, C12 : 0 3-OH, C16 : 1ω7c, C18 : 1ω7c and C19 : 0 cyclo ω8c. Both strains could utilize diesel oil and a variety of xenobiotics as carbon and energy sources. The results of physiological, biochemical, chemotaxonomic and molecular analyses allowed the clear differentiation of strains JC109T and JC261 from all other members of the genus Alcanivorax . Strains JC109T and JC261 are thus considered to represent a novel species, for which the name Alcanivorax xenomutans sp. nov. is proposed. The type strain is JC109T ( = KCTC 23751T = NBRC 108843T).
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Viana, Andrwey Augusto Galvão, Bianca Teixeira Morais de Oliveira, Thiago Gonçalves Cavalcanti, Kally Alves de Sousa, Elisângela Afonso de Moura Mendonça, Ian Porto Gurgel do Amaral, and Ulrich Vasconcelos. "Correlation between pyocyanin production and hydrocarbonoclastic activity in nine strains of Pseudomonas aeruginosa." International Journal of Advanced Engineering Research and Science 5, no. 7 (2018): 212–23. http://dx.doi.org/10.22161/ijaers.5.7.28.

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Dashti, Narjes, Nedaa Ali, Samar Salamah, Majida Khanafer, Ghada Al‐Shamy, Husain Al‐Awadhi, and Samir S. Radwan. "Culture‐independent analysis of hydrocarbonoclastic bacterial communities in environmental samples during oil‐bioremediation." MicrobiologyOpen 8, no. 2 (April 15, 2018): e00630. http://dx.doi.org/10.1002/mbo3.630.

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van Beilen, Jan B., Mercedes M. Marin, Theo H. M. Smits, Martina Rothlisberger, Alessandro G. Franchini, Bernard Witholt, and Fernando Rojo. "Characterization of two alkane hydroxylase genes from the marine hydrocarbonoclastic bacterium Alcanivorax borkumensis." Environmental Microbiology 6, no. 3 (March 2004): 264–73. http://dx.doi.org/10.1111/j.1462-2920.2004.00567.x.

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38

Gentile, G., V. Bonasera, C. Amico, L. Giuliano, and M. M. Yakimov. "Shewanella sp. GA-22, a psychrophilic hydrocarbonoclastic antarctic bacterium producing polyunsaturated fatty acids." Journal of Applied Microbiology 95, no. 5 (November 2003): 1124–33. http://dx.doi.org/10.1046/j.1365-2672.2003.02077.x.

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39

Chettri, Bobby, Ningombam A. Singha, Arghya Mukherjee, Amar Nath Rai, Dhrubajyoti Chattopadhyay, and Arvind Kumar Singh. "Hydrocarbon degradation potential and competitive persistence of hydrocarbonoclastic bacterium Acinetobacter pittii strain ABC." Archives of Microbiology 201, no. 8 (June 5, 2019): 1129–40. http://dx.doi.org/10.1007/s00203-019-01687-z.

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40

Sabirova, Julia S., Manuel Ferrer, Daniela Regenhardt, Kenneth N. Timmis, and Peter N. Golyshin. "Proteomic Insights into Metabolic Adaptations in Alcanivorax borkumensis Induced by Alkane Utilization." Journal of Bacteriology 188, no. 11 (June 1, 2006): 3763–73. http://dx.doi.org/10.1128/jb.00072-06.

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ABSTRACT Alcanivorax borkumensis is a ubiquitous marine petroleum oil-degrading bacterium with an unusual physiology specialized for alkane metabolism. This “hydrocarbonoclastic” bacterium degrades an exceptionally broad range of alkane hydrocarbons but few other substrates. The proteomic analysis presented here reveals metabolic features of the hydrocarbonoclastic lifestyle. Specifically, hexadecane-grown and pyruvate-grown cells differed in the expression of 97 cytoplasmic and membrane-associated proteins whose genes appeared to be components of 46 putative operon structures. Membrane proteins up-regulated in alkane-grown cells included three enzyme systems able to convert alkanes via terminal oxidation to fatty acids, namely, enzymes encoded by the well-known alkB1 gene cluster and two new alkane hydroxylating systems, a P450 cytochrome monooxygenase and a putative flavin-binding monooxygenase, and enzymes mediating β-oxidation of fatty acids. Cytoplasmic proteins up-regulated in hexadecane-grown cells reflect a central metabolism based on a fatty acid diet, namely, enzymes of the glyoxylate bypass and of the gluconeogenesis pathway, able to provide key metabolic intermediates, like phosphoenolpyruvate, from fatty acids. They also include enzymes for synthesis of riboflavin and of unsaturated fatty acids and cardiolipin, which presumably reflect membrane restructuring required for membranes to adapt to perturbations induced by the massive influx of alkane oxidation enzymes. Ancillary functions up-regulated included the lipoprotein releasing system (Lol), presumably associated with biosurfactant release, and polyhydroxyalkanoate synthesis enzymes associated with carbon storage under conditions of carbon surfeit. The existence of three different alkane-oxidizing systems is consistent with the broad range of oil hydrocarbons degraded by A. borkumensis and its ecological success in oil-contaminated marine habitats.
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Méndez, Valentina, Sebastián Fuentes, Verónica Morgante, Marcela Hernández, Myriam González, Edward Moore, and Michael Seeger. "Novel hydrocarbonoclastic metal-tolerant Acinetobacter and Pseudomonas strains from Aconcagua river oil-polluted soil." Journal of soil science and plant nutrition 17, no. 4 (December 2017): 1074–87. http://dx.doi.org/10.4067/s0718-95162017000400017.

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42

Njoki Mwaura, Agnes. "Screening and Characterization of Hydrocarbonoclastic Bacteria Isolated from Oil-contaminated Soils from Auto Garages." International Journal of Microbiology and Biotechnology 3, no. 1 (2018): 11. http://dx.doi.org/10.11648/j.ijmb.20180301.13.

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43

Thavasi, R., S. Jayalakshm, R. Radhakrish, and T. Balasubram. "Plasmid Incidence in Four Species of Hydrocarbonoclastic Bacteria Isolated from Oil Polluted Marine Environment." Biotechnology(Faisalabad) 6, no. 3 (June 15, 2007): 349–52. http://dx.doi.org/10.3923/biotech.2007.349.352.

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44

Lange, Alvin Brian, Klaus Bernhard Tenberge, Horst Robenek, and Alexander Steinbüchel. "Cell surface analysis of the lipid-discharging obligate hydrocarbonoclastic species of the genus Alcanivorax." European Journal of Lipid Science and Technology 112, no. 6 (May 20, 2010): 681–91. http://dx.doi.org/10.1002/ejlt.201000048.

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45

Teramoto, Maki, Shu Yeong Queck, and Kouhei Ohnishi. "Specialized Hydrocarbonoclastic Bacteria Prevailing in Seawater around a Port in the Strait of Malacca." PLoS ONE 8, no. 6 (June 18, 2013): e66594. http://dx.doi.org/10.1371/journal.pone.0066594.

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46

Kadri, Tayssir, Sara Magdouli, Tarek Rouissi, Satinder Kaur Brar, Rimeh Daghrir, and Jean-Marc Lauzon. "Bench-scale production of enzymes from the hydrocarbonoclastic bacteria Alcanivorax borkumensis and biodegradation tests." Journal of Biotechnology 283 (October 2018): 105–14. http://dx.doi.org/10.1016/j.jbiotec.2018.07.039.

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47

Kurtz, Alexandra Massey, and Sidney A. Crow Jr. "Transformation of Chlororesorcinol by the Hydrocarbonoclastic Yeasts Candida maltosa , Candida tropicalis , and Trichosporon oivide." Current Microbiology 35, no. 3 (September 1, 1997): 165–68. http://dx.doi.org/10.1007/s002849900232.

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48

Sheppard, Petra J. "The Importance of Weathered Crude Oil as a Source of Hydrocarbonoclastic Microorganisms in Contaminated Seawater." Journal of Microbiology and Biotechnology 22, no. 9 (September 28, 2012): 1185–92. http://dx.doi.org/10.4014/jmb.1201.01049.

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49

Lewis, Dawn E., Ashish Pathak, Cynthia B. Jones, Charlemagne Akpovo, and Ashvini Chauhan. "Metagenomic evaluation of a Utah tar sand microbiota suggests the predominant hydrocarbonoclastic role of Actinobacteria." F1000Research 7 (October 17, 2018): 1650. http://dx.doi.org/10.12688/f1000research.16126.1.

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Background: Occurrences of tar sands have been reported in 22 states in the United States; however, the largest deposit is located in southwestern Utah. It has been suggested that tar sands were created by the microbial degradation of immobile subsurface oil over several million years; however, little is known about the indigenous microbial communities in the bituminous tar sands. Methods: This study identified Utah tar sand microbiota using next-generation sequencing technology and characterized the functional diversity using community-level physiological profile (CLPP). Results: Microbiota identified in these tar sands are mainly affiliated with the Gram-positive Actinobacteria and representatives of genera that have also been previously shown to degrade aromatic hydrocarbons, including Arthrobacter, Dietzia, Janibacter, Nocardioides, Microbacterium, Agrococcus and Salinibacterium, suggesting that these microbes likely play roles in the biodegradation of oil-hydrocarbons. CLPP analysis revealed less than 24 h was needed for the first color development in the microplate wells containing the polymers, whereas the duration of the lag phase of the carboxylic acids was prolonged. Conclusions: The quick utilization of the polymers suggests that the indigenous microbial community, especially the actinomycetes in the tar sand habitat, are poised and primed to degrade these recalcitrant compounds.
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I Zam, Syukria, and irfan mustafa. "DEGRADATION OF PETROLEUM REFINERY WASTE BY A CONSORTIUM OF HYDROCARBONOCLASTIC BACTERIA ON SEVERAL C:N:P RATIO." Journal of Tropical Life Science 2, no. 1 (January 1, 2012): 11–14. http://dx.doi.org/10.11594/jtls.02.01.03.

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