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1

K.Ramanji, Reddy* Dr.Chandaka Madhu Dr.J.Balaji Chandra Mouli Dr.B.Jagan Mohan Reddy Penumala Joshi Ballavarapu Sharanya. "COMPARATIVE STUDY ON PHYTOCHEMICAL SCREENING AND ANTIOXIDANT ACTIVITY ON ETHANOLIC EXTRACT OF MUSA ACUMINATE AND MANGIFERA INDICA." Indo American Journal of Pharmaceutical Sciences 04, no. 09 (2017): 2817–29. https://doi.org/10.5281/zenodo.888116.

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The present study was undertaken to investigate in vitro antioxidant activity of alcoholic extract of musa acuminata and mangifera indica . Method and methodlogy: The total Phenolic content was determined using folinciocalteau method while the total flavanoid content was determined using aluminum chloride method. In vitro antioxidant activity was evaluated using the Reducing power assay , Hydrogen peroxide scavenging assay, nitric oxide scavenging activity, and DPPH scavenging activity. Result: In the present study we have conclude that Mangifera Indica has a significant activity than Musa Acu
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Dr.ChandakaMadhu, Gunugurthi Ramya Sai Shreeja Alikette Deeti Prasanna Adiraju Usha Lalitha Gollapalli Shivapoojitha Meher Fathima. "COMPARATIVE STUDY ON PHYTOCHEMICAL SCREENING AND ANTIOXIDANT ACTIVITY ON ETHANOLIC EXTRACT OF ACTINIDIA DELICIOSA AND VITIS VINFERA." INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES 05, no. 01 (2018): 735–44. https://doi.org/10.5281/zenodo.1174193.

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The present study was undertaken to investigate in vitro antioxidant activity of alcoholic extract of Actinidia Deliciosa And Vitis Vinfera. Method and methodlogy: The total Phenolic content was determined using folinciocalteau method while the total flavanoid content was determined using aluminum chloride method. In vitro antioxidant activity was evaluated using the Reducing power assay , Hydrogen peroxide scavenging assay, nitric oxide scavenging activity, and DPPH scavenging activity. Result: In the present study we have conclude that Vitis Vinfera, has a significant activity than Actinidia
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M, Yadav, Kumari S, Nagraj N, et al. "Hydrogen Peroxide Scavenging by Methanolic Extracts of Coriander: An In Vitro Antioxidant Study." Pharmaceutical Drug Regulatory Affairs Journal 8, no. 1 (2025): 1. https://doi.org/10.23880/pdraj-16000162.

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Most of the nutraceutical plants, plants associated products and foods are known for their promising antioxidant properties due to the presence of several metabolites like polyphenolic compounds, flavonoids, terpenoids, vitamin C and many other. These molecules have strong tendency to scavenge the free radicals (reactive oxygen species) generated inside our body system due to the various reasons. Out of several methods for the antioxidant activity determination; hydrogen peroxide scavenging activity is an important method. This method has been used to determine the percentage scavenging activi
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Paździoch-Czochra, Marzanna, and Anna Wideńska. "Spectrofluorimetric determination of hydrogen peroxide scavenging activity." Analytica Chimica Acta 452, no. 2 (2002): 177–84. http://dx.doi.org/10.1016/s0003-2670(01)01455-6.

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Enechi, Osmund Chukwuma Okeke Emmanuel Sunday* Abonyi Uchenna Collins Nwankwo Nicodemus Emeka Ndimele Ugochi Joy Benneth Moses Ebere. "EVALUATION OF IN VITRO ANTI-INFLAMMATORY AND ANTIOXIDANT POTENTIALS OF METHANOL LEAF EXTRACT OF FAGARA ZANTHOXYLOIDES." INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES o6, no. 08 (2019): 14365–71. https://doi.org/10.5281/zenodo.3360753.

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<strong><em>Background:</em></strong><em> Fagara zanthoxyloides is a medicinal plant used in traditional medicine for the treatment of elephantiasis, toothache, sexual impotence, malaria, dysmenorrheal and abdominal pain among rural dwellers.</em> <strong><em>Objective:</em></strong><em> To study the In vitro anti-inflammatory and antioxidant activities of methanol extract of Fagara zanthoxyloides leaves.</em> <strong><em>Materials and Methods:</em></strong><em> Methanol extract of Fagara zanthoxyloides leaves were used for this study. In vitro anti-inflammatory studies were performed for the
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Rayadurgam, Sireesha, and Manikandan Krishnan. "Polyherbal Formulation for Atherosclerosis: GC-MS Fingerprinting, In-vivo Antioxidant and Antibacterial Activities." INTERNATIONAL JOURNAL OF PHARMACEUTICAL QUALITY ASSURANCE 15, no. 01 (2024): 88–93. http://dx.doi.org/10.25258/ijpqa.15.1.13.

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Atherosclerosis is a prolonged inflammatory disease affecting large and medium-sized arteries, requiring a phytotherapeutic approach that incorporates multiple bioactive elements and a multi-target strategy. A novel polyherbal formulation has been formulated to treat atherosclerosis. The methanolic extract of the formulation has been characterized using gas chromatography–mass spectrometry (GC-MS) fingerprinting analysis. The extract was further screened for antioxidant activity like DPPH radical scavenging assay, hydrogen peroxide scavenging activity, intracellular reactive oxygen species (RO
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Li, Qiong Jie, and Chun Ling Xiao. "Study on Antioxidant Activity of Pigment in Black Soybean Peel." Advanced Materials Research 881-883 (January 2014): 378–83. http://dx.doi.org/10.4028/www.scientific.net/amr.881-883.378.

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With black soybean as raw materials, 50% ethanol as the extractant, at 70°C, pH1.0, solid-liquid ratio of 1:100(g:mL), at 90min were used to extract the pigment of black soybean Through the establishment of DPPH•,•OH, superoxide anion free radicals, reducing power and hydrogen peroxide. The results show: its ability of scavenging DPPH•, •OH, hydrogen peroxide is far better than ascorbic acid. The pigment at concentration of 0.6mg/mL shows a scavenging rate for DPPH• up to 88.29%,and for •OH 89.36%,for hydrogen peroxide89.75%.In addition,the general reducing power and scavenging rate for supero
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8

Kharat, Amol R., Farooqui Zakerab, and Kiran Ramesh Kharat. "Free Radical Scavenging Potential of Mentha arvensis of South Gujarat: Evidence from In-vitro Assay." Journal of Drug Delivery and Therapeutics 9, no. 3 (2019): 263–68. http://dx.doi.org/10.22270/jddt.v9i3.2866.

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Present work was undertaken to evaluate efficacy of aqueous and methanol extracts Mentha arvensis leaves as free radical scavenger. The free radical scavenging activity was evaluated by nitric oxide scavenging method, hydrogen peroxide scavenging method and ferric thiocyanate method. The result of the studies was compared with the standard solution of Ascorbic Acid (Vitamin C) treated with same reagent. The results of all the studies showed significant free radical scavenging activity in both aqueous and methanolic extracts of Mentha arvensis and methanolic extract showed the highest free radi
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Macáková, Kateřina, Lubomir Opletal, Miroslav Polášek, Věra Samková, and Luděk Jahodář. "Free-radical Scavenging Activity of Some European Boletales." Natural Product Communications 4, no. 2 (2009): 1934578X0900400. http://dx.doi.org/10.1177/1934578x0900400219.

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The ethanol extracts of 47 mushroom species from the order Boletales were tested for their free radical scavenging activities by means of the DPPH free-radical scavenging and hydrogen peroxide-induced luminol chemiluminescence assays. In the DPPH radical scavenging test, 26 species gave EC50 values of less than 1 mg/mL. The highest activity was exhibited by Gyrodon lividus (0.05 mg/mL). However, in the hydrogen peroxide-induced luminol chemiluminescence assay, only 4 species had EC50 values of less than 1 mg/mL, with the highest activity being exhibited by Strobilomyces floccopus (0.26 mg/mL).
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Macáková, Kateřina, Lubomír Opletal, Miroslav Polášek, and Věra Samková. "Free-radical Scavenging Activity of some European Polyporales." Natural Product Communications 5, no. 6 (2010): 1934578X1000500. http://dx.doi.org/10.1177/1934578x1000500623.

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The ethanol extracts of 30 mushroom species from the order Polyporales were tested for their free radical scavenging activities by means of the DPPH free-radical scavenging and hydrogen peroxide-induced luminol chemiluminescence assays. In the DPPH radical scavenging test, 7 species gave EC50 values of less than 1 mg/mL. The highest activity was exhibited by Fomitopsis pinicola (0.09 mg/mL). However, in the hydrogen peroxide-induced luminol chemiluminescence assay, only 4 species had EC50 values of less than 1 mg/mL, with the highest activity being exhibited also by F. pinicola (0.26 mg/mL). W
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Siriwardhana, Nalin, K. W. Lee, Y. J. Jeon, S. H. Kim, and J. W. Haw. "Antioxidant Activity of Hizikia fusiformis on Reactive Oxygen Species Scavenging and Lipid Peroxidation Inhibition." Food Science and Technology International 9, no. 5 (2003): 339–46. http://dx.doi.org/10.1177/1082013203039014.

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Water and organic extracts (diethyl ether, chloroform, ethyl acetate, acetone, ethanol and methanol) obtained from Hizikia fusiformis were screened on reactive oxygen species (ROS) scavenging assays (1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide anion, hydrogen peroxide and hydroxyl radical) and lipid peroxidation (inhibition of linoleic acid oxidation) inhibitory assays. Water, methanol and ethanol extracts showed significant ROS radical scavenging activities. Water extracts showed high scavenging activities on hydrogen peroxide (around 76%) and DPPH radicals (around 75%) while it presente
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Ambe, D. A., C. E. Odiete, J. D. Buba, and E. E. Odion. "Antioxidant Effects of Cocos nucifera L. (Asteraceae) Stem Bark Extracts using Diphenyl-I-picrylhydrazyl; Hydrogen Peroxide and Nitric Oxide Scavenging Assay." Journal of Applied Sciences and Environmental Management 27, no. 10 (2023): 2291–95. http://dx.doi.org/10.4314/jasem.v27i10.21.

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Coconut (Cocos nucifera L. (Arecaceae)) supplies nearly all of the basics of life, including food, oil and phyto-medicines. However, reports on the antioxidant effects of the stem bark of C. nucifera are scanty. Therefore, the objective of this study is to evaluate the antioxidant effects of coconut stem bark extracts using diphenyl-i-picrylhydrazyl (DPPH); hydrogen peroxide (H2O2) and nitric oxide (NO) scavenging assay using standard techniques The stem bark of the plant was pulverized and macerated with 70% ethanol. Antioxidant analysis was evaluated on the extract using diphenyl-I-picrylhyd
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Sharma, Rajesh Kumar, Rajni Singh, K. K. Jha, and Abhishek B. "Antibacterial and Antioxidant activity of Benincasa hispida using Hydrogen peroxide scavenging model." Indian Journal of Pharmaceutical and Biological Research 2, no. 01 (2014): 86–94. http://dx.doi.org/10.30750/ijpbr.2.1.14.

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The seeds of Benincasa hispida commonly known as ash gourd belonging to Cucurbitaceae family is employed as a main ingredient in kusmanda lehyam in Ayurvedic system of medicine. The seeds of Benincasa hispida mashed with milk or the various preparations from the pulp of fruit in the form of sweetmeats, like Kusmanda paka and petha are commonly used as a general tonic, aphrodisiac, rejuvenative and also a brain tonic. The plant of Benincasa hispida was collected from (Central Council for Research in Ayurveda and Siddha, Dept. of ayush, Ministry of Health and F.W, Govt. of India New Delhi) Govt.
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Mohammedi, Zohra. "Extraction, Phenolic Content and Hydrogen Peroxide Scavenging Capacity of Extracts from Some Honey Samples, Propolis and Bee Pollen." Biology, Medicine, & Natural Product Chemistry 10, no. 1 (2021): 27–32. http://dx.doi.org/10.14421/biomedich.2021.101.27-32.

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Honey and propolis is natural food, produced by honey bees (Apis mellifera) and largely used by the local population for its medicinal properties. Our work aims to extract and evaluate the hydrogen peroxide scavenging capacity of different phenolic extracts from some bee products. Phenolic compounds from honey samples, propolis, and bee pollen were extracted by methanol and subjected to radical scavenging activity towards hydrogen peroxide. The results showed the highest values for the total phenolic and total flavonoïd contents in propolis and bee pollen, and a great hydrogen peroxide (H2O2)
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Nagaretnam, Prahadeesh, and Arulmoly Kiruthika. "Structure activity Relationship Study of Simple Hydroxyl Coumarins as Antioxidant to Scavenge Hydrogen peroxide." RESEARCH REVIEW International Journal of Multidisciplinary 4, no. 6 (2019): 675–82. https://doi.org/10.5281/zenodo.3275482.

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Coumarins are heterocyclic compounds; structurally constructed by &alpha;-pyrone ring, fused with benzene ring. Coumarins are very interested because of their abundance in nature and their tremendous pharmacological properties such as anticoagulant, anticancer, antibacterial, anti-inflammatory, anti-tuberculosis, antioxidant, etc.The need for the antioxidant is very important because of the role reactive oxygen species and its contribution to many deleterious effects in human body. Hydrogen peroxide is such a reactive oxygen species. This study focused on synthesizing the parent compound,Couma
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Chika C Abba, Chineze B Nsofor, Chioma C Udeozor, et al. "In-vitro comparative study of solvent effect on antioxidant properties of leaf extracts of Anthocleista djalonensis." Magna Scientia Advanced Biology and Pharmacy 11, no. 2 (2024): 072–79. http://dx.doi.org/10.30574/msabp.2024.11.2.0020.

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This study demonstrated that the extraction solvents play an important role in the extraction of important bioactive compounds with antioxidant properties from the leaves of Anthocleista djalonensis. Anthocleista djalonensis, a plant native to tropical Africa, is believed to have antioxidant properties due to its bioactive composition. The powdered leaves were macerated using local gin, hot distilled water, and analytical ethanol to extract bioactive compounds. The in vitro antioxidant activity of these extracts was assessed using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assays, nitric
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Anandakumar, Arunkumar, Ananthan Gnanakkan, and Murugan Rajaram. "Antioxidant Activity of astaxanthin isolated from Charybdis Edwardsii Leene and Buitendijk ,1949 from Pazhayar, Southeast Coast of India." International Journal of Trend in Scientific Research and Development 2, no. 5 (2018): 25–29. https://doi.org/10.31142/ijtsrd15762.

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Astaxanthin extracted from Red Crab Charybdis edwardsii was assayed for antioxidant activity viz., total antioxidant, reducing power, hydrogen peroxide radical scavenging assay, and DPPH free radicals scavenging activity. Final concentration of astaxanthin was quantified to be 49.05 &micro;g g using the standard astaxanthin. The antioxidant activity was found to be 60 , hydrogen peroxide scavenging activity showed 89 inhibition, whereas 1,1 diphenyl 2 picrylhydrazl DPPH activity showed 86 of scavenging activity. Present investigation proves that the astaxanthin from C. edwardsii acts as a prom
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Ilechukwu, C. C., C. O. Ezenwelu, J. C. Ifemeje, C. Okechukwu, J. N. Ilechukwu, and I. H. Iheukwumere. "In Vitro Antioxidant and Phytochemical Analysis of Solanum aethiopicum (Garden Egg Leaf) and Telfairia occidentalis (Fluted Pumpkin Leaf)." IPS Interdisciplinary Journal of Biological Sciences 4, no. 1 (2025): 107–11. https://doi.org/10.54117/iijbs.v4i3.56.

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This study evaluated and compared the in vitro antioxidant activity and phytochemical constituents of methanol leaf extracts of Solanum aethiopicum and Telfairia occidentalis. The phytochemical analysis of both Solanum aethiopicum and Telfairia occidentalis revealed the presence of alkaloids, saponins, flavonoids, terpenoids, glycosides, and steroids in both plant extracts, whereas tannins were absent in Solanum aethiopicum but present in Telfairia occidentalis. The results showed that the methanol leaf extracts of T. occidentalis and S. aethiopicum were able to scavenge nitric oxide in a dose
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Zhang, Ning, Zhen Yu Wang, Hua Zhang, Jian Ren, and Xiu Mei Hao. "In Vitro Antioxidant Activities of Polysaccharides from Auricularia auriada." Advanced Materials Research 183-185 (January 2011): 104–9. http://dx.doi.org/10.4028/www.scientific.net/amr.183-185.104.

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This study aims to evaluate the antioxidative activities of water and alkaline solution pH=8, 50% ethanolic (APP1 or SAPP1), 75% ethanolic (APP2 or SAPP2) and 100% ethanolic (APP3 or SAPP3) extracts of A. auricular. The antioxidant activities, including the ABTS, superoxide anion radical-scavenging effect, hydrogen peroxide radical-scavenging effect and chelating ability on ferrous ions were studied in vitro. A comparison of the 50% effective concentration (EC50) values of different antioxidative reactions revealed that SAPP3 was more effective in scavenging ABTS, hydrogen peroxide and chelati
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Prasad.M.P, Prasad M. P., and Ashwin Rajkumar.R. "Determination of Antioxidant Activity of Citrus Species using Different Solvent Extracts by Hydrogen Peroxide Scavenging Method." International Journal of Scientific Research 3, no. 6 (2012): 37–38. http://dx.doi.org/10.15373/22778179/june2014/15.

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Raja, Boobalan, and Kodukkur Pugalendi. "Evaluation of antioxidant activity of Melothria maderaspatana in vitro." Open Life Sciences 5, no. 2 (2010): 224–30. http://dx.doi.org/10.2478/s11535-010-0005-5.

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AbstractIn this study, an aqueous extract of leaves from Melothria maderaspatana was tested for in vitro antioxidant activity. Free radical scavenging assays, such as hydroxyl radical, hydrogen peroxide, superoxide anion radical and 2,2-diphenyl-1-picryl hydrazyl (DPPH), 2,2’-azinobis-(3-ethyl-enzothiazoline-6-sulfonic acid) (ABTS) radical scavenging, and reducing power assay, were studied. The extract effectively scavenged hydroxyl radical, hydrogen peroxide and superoxide anion radicals. It also scavenged DPPH and ABTS radicals. Furthermore, it was found to have reducing power. All concentra
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Ganie, Showkat Ahmad, Ehtishamul Haq, Akbar Masood, Abid Hamid, and Mohmmad Afzal Zargar. "Antioxidant and Protective Effect of Ethyl Acetate Extract ofPodophyllum hexandrumRhizome on Carbon Tetrachloride Induced Rat Liver Injury." Evidence-Based Complementary and Alternative Medicine 2011 (2011): 1–12. http://dx.doi.org/10.1155/2011/238020.

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The antioxidant and hepatoprotective activities of ethyl acetate extract was carefully investigated by the methods of DPPH radical scavenging activity, Hydroxyl radical scavenging activity, superoxide radical scavenging activity, hydrogen peroxide radical scavenging activity, and its reducing power ability. All thesein vitroantioxidant activities were concentration dependent, which were compared with standard antioxidants such as BHT, α-tocopherol. The hepatoprotective potential ofPodophyllum hexandrumextract was also evaluated in male Wistar rats against carbon tetrachloride- (CCl4-) induced
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Attama, Chika, Lawrence Luka, and Chidama, Bulama Ndakudu. "Chemical characterization and antioxidant activities of essential oil of Ficus elastica Roxb. ex Hornem. leaves." International Journal of Secondary Metabolite 11, no. 3 (2024): 562–73. http://dx.doi.org/10.21448/ijsm.1377543.

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The present study investigates the chemical constituents and antioxidant potential of essential oils extracted from Ficus elastica. The essential oils were obtained through steam distillation and were subjected to gas chromatography-mass spectrometry (GC-MS) analysis to determine their constituents representing 100% of the total peak areas. The analysis revealed the presence of 34 compounds. Among these compounds, Caryophyllene was found to be the major constituent, accounting for 50.57% of the total percentage area. Among other identified major compounds include γ-Muurolene (8.19%), camphene
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Dong, Yuanyang, Qihang Hou, Meng Sun, Jingjing Sun, and Bingkun Zhang. "Targeted Isolation of Antioxidant Constituents from Plantago asiatica L. and In Vitro Activity Assay." Molecules 25, no. 8 (2020): 1825. http://dx.doi.org/10.3390/molecules25081825.

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Plantago asiatica L. is widely distributed in Eastern Asia and a commonly used drug in China, Korea, and Japan for diuretic and antiphlogistic purposes. In this experiment, the present study was performed to isolate antioxidant molecules based on the DPPH scavenging activity assay and discover the bioactive compounds which contributed to performing the function of Plantago asiatica L. Each faction was chosen for further isolation guided by DPPH scavenging activity assay. Afterwards, two potential bioactive molecules, aesculetin and apigenin, were isolated for in vitro antioxidant activity in c
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Rizfa, Mila Safitri, Ervia Yudiati, and Diah Permata Wijayanti. "Improving The Antioxidant Activity of Sodium Alginate from Sargassum sp. by Thermal Heating and Chemical Methods." Jurnal Kelautan Tropis 23, no. 3 (2020): 284–90. http://dx.doi.org/10.14710/jkt.v23i3.8946.

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The relationship between molecular structure and bioactivity was evaluated for sodium alginates obtained under different degradation treatment (raw alginates, heat-treated, and chemical-treated) from Sargassum sp. This study was conducted to identify the antioxidant activities of the degraded sodium alginate from Sargassum sp. compared to raw extract. Raw alginate as the control treatment was dried overnight at 60 °C, while heat-treated was heated raw alginate at 140oC in a laboratory oven (4.5 hours). Two chemical-treated were applied. Raw alginate added hydrogen peroxide and raw alginat with
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Huang, Liang, Chunxia Li, Ning Sun, et al. "Optimization of Liquid Culture Condition of a Novel Fungus Hygrophoropsis sp. and Antioxidant Activity of Extracts." Biochemistry Research International 2020 (August 1, 2020): 1–11. http://dx.doi.org/10.1155/2020/7403257.

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To evaluate pharmacological activities of a novel fungus Hygrophoropsis sp., the influence of aeration rate on the production of mycelial biomass, exopolysaccharides (EPS), and intrapolysaccharides (IPS) in the fungus Hygrophoropsis sp. was investigated. And the water extracts of cultured Hygrophoropsis sp. mycelia and the fermentation broth were analyzed for their antioxidation activity by using four different assay methods such as hydroxyl radical scavenging, superoxide radical scavenging, hydrogen peroxide scavenging, and reducing power. The Hygrophoropsis sp. was cultivated under various a
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Ekanayake, P., Y. D. Lee, and J. Lee. "Antioxidant Activity of Flesh and Skin of Eptatretus Burgeri (Hag Fish) and Enedrias Nebulosus (White Spotted Eel)." Food Science and Technology International 10, no. 3 (2004): 171–77. http://dx.doi.org/10.1177/1082013204044822.

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Ethyl acetate and diethyl ether extracts, previously extracted with methanol, were obtained from flesh and skin of Eptatretus burgeri (hag fish) and Enedrias nebulosus (white spotted eel). Eight different extracts (4 mg/mL) were tested for DPPH free radical scavenging activity and the values were compared with commercial antioxidants (α-tocopherol and BHT). All extracts of E. burgeri exhibited significantly positive results (&gt; 65%) in scavenging DPPH radicals compared to E. nebulosus. Thus, only E. burgeri extracts were tested for dose effect, hydrogen peroxide, hydroxyl radical, superoxide
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E. C., Moneme,, Onochie, A. U., Onuegbu, M. E., Anyanwu, R. O., Ajakpofo, F. O., and Oladejo, A. A. "In vitro Antioxidant Activity and Free Radical Scavenging Abilities of Ocimum gratissimum Leaf Extract." Journal of Advances in Medical and Pharmaceutical Sciences 27, no. 3 (2025): 54–63. https://doi.org/10.9734/jamps/2025/v27i3758.

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In recent years, researchers have intensified the search for effective, non-toxic, natural edible plants with free radical scavenging activity whose effects are cushioned by antioxidant molecules. This study evaluated the in vitro antioxidant activity and free radical scavenging abilities of Ocimum gratissimum leaf extract. In vitro antioxidant (nitric oxide, Hydrogen Peroxide, Ferric Reducing property and DPPH) activities were determined using standard methods. Results showed that the leaf extract of Ocimum gratissimum considerably scavenges radicals of nitric oxide, hydrogen peroxide and DPP
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Dahiru, Naziru, Ritu Paliwal, Muhammad Abubakar Madungurum, Abdurrahman Siraj Abubakar, and Bilkisu Abdullahi. "Study on Antioxidant Property of Syzygium aromaticum (Clove)." Journal of Biochemistry, Microbiology and Biotechnology 10, no. 1 (2022): 13–16. http://dx.doi.org/10.54987/jobimb.v10i1.657.

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This paper is aimed at evaluating the antioxidant properties of Eugenia Caryophyllata (Syzygium aromaticum), 100% aqueous and 20:80% (water and alcohol) for hydroethanolic and hydromethanolic extracts were prepared as working solutions for the studies. About 5 different antioxidant examinations of Syzygium aromaticum (Clove) extracts were carried out which include total phenolic content, antioxidant assay, free radical’s DPPH scavenging activity, scavenging hydrogen peroxide and reducing power assay, which was estimated spectrophotometrically by the use of butylated hydroxyanisole (BHA) as sta
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Reddy, Amala, and Ganesh Venkatayappa. "FREE RADICAL QUENCHING EFFICACY OF VARIOUS EXTRACTS OF C.PICTUS TO COMBAT OXIDATIVE DAMAGE/STRESS: AN IN VITRO STUDY." Asian Journal of Pharmaceutical and Clinical Research 10, no. 2 (2017): 178. http://dx.doi.org/10.22159/ajpcr.2017.v10i2.15011.

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Objectives: The objectives of this research article are to elucidate the antioxidant activity of the sequentially extracted fractions using solvents with a varying range of polarity from the leaves of Costus pictus (Zingiberaceae). The antioxidant efficacy to combat the oxidative stress was evaluated based on their superoxide, hydrogen peroxide, and nitric oxide (NO) scavenging activity of the leaf extract.Methods: Extraction was performed by sequential extraction methods with low to high polar solvents. Using hexane as a low polar, dichloromethane as medium polar, and ethyl acetate and methan
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Olaitan, Michael O., Cosmas O. Ujowundu, Chiamaka P. Nzebude, et al. "Organic wastes of Citrus sinensis Peels- a source of eco-friendly and sustainable bioactive compounds for promoting health." Asian Journal of Biochemistry, Genetics and Molecular Biology 16, no. 2 (2024): 21–31. http://dx.doi.org/10.9734/ajbgmb/2024/v16i2358.

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To determine the phytochemicals, radical scavenging and antioxidant potential of orange peel extract. Citrus sinensis were subjected to extraction with ethanol. Gas chromatography (GC) was utilized to determine the phytochemical composition of orange peel extract. Hydrogen peroxide, superoxide, nitric oxide, and hydroxyl radical scavenging assays were conducted to assess radical scavenging potential of the extract. Antioxidant activities of the peel extracts were determined via the 2,2-diphenylpicrylhydrazyl (DPPH) free radical scavenging activity, ferric reducing antioxidant power (FRAP) assa
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*Naila, Ali *Humera Afrasiab *Farkhanda Jabeen. "ANTIOXIDANT ACTIVITY OF METHANOLIC EXTRACTS OF INDIGENOUS AND EUROPEAN GRAPE VARIETIES FROM PAKISTAN." INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES 05, no. 09 (2018): 9431–39. https://doi.org/10.5281/zenodo.1443424.

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<em>Grapes are rich in phenolic compounds, both flavonoids and non-flavonoids. The aim of the present study is to evaluate antioxidant of methanol extract of Grape leaves of different indigineous and European varieties of Pakistan. The results showed that grape leaves extract had clear antioxidants activities. </em><em>The antioxidant activities of all the extracts were examined usung three complementary methods, namely 1,1-diphenyl-2-picrylhydrazyl (DPPH)&nbsp;assay, Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)scavenging assay and reducing power scavenging assay. Although all the varieties
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Benmeziane, F. "Hydrogen peroxide scavenging activity of grape (Vitis vinifera) methanolic extract." Food Research 1, no. 2 (2017): 39–42. http://dx.doi.org/10.26656/fr.2017.2.005.

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Costa, David, Ana Gomes, Salette Reis, José L. F. C. Lima, and Eduarda Fernandes. "Hydrogen peroxide scavenging activity by non-steroidal anti-inflammatory drugs." Life Sciences 76, no. 24 (2005): 2841–48. http://dx.doi.org/10.1016/j.lfs.2004.10.052.

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Deore, Rutuja K., Khemchand Surana, Sunil K. Mahajan, Rushikesh L. Bachhav, and Deepak D. Sonawane. "Development and Evaluation of Moisturizing Cream containing Salvia hispanica and Aloe vera and its Antioxidant Potential." Ars Pharmaceutica (Internet) 66, no. 3 (2025): 276–87. https://doi.org/10.30827/ars.v66i3.32785.

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Introduction: Bioactive chemicals in Salvia hispanica (Chia) seeds and Aloe vera hydrate, protect, and restore skin. The goal of this work will formulate and evaluate a multifunctional herbal cream with Salvia hispanica seed mucilage, Aloe vera gel, shea butter, and coconut oil to hydrate and restore skin. Methods: Salvia hispanica seed extract was obtained through mucilage extraction. Aloe vera gel was prepared and incorporated into the formulation along with shea butter and coconut oil. The final cream formulation was evaluated for physicochemical parameters such as pH (6), spreadability (7.
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Vijaya, Lakshmi Nandikatti* Pravalika Amanik Sravangi Lavanya M. "Anti –Oxidant Activity of Methanolic Extract of Azadirachta Indica Flowers." International Journal of Pharmaceutical Sciences 3, no. 5 (2025): 4333–37. https://doi.org/10.5281/zenodo.15517011.

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Azadirachta indica, commonly known as Neem, is a fast-growing, evergreen tree indigenous to India. All parts of the plant, including leaves, bark, seeds, roots, and flowers, are widely used in traditional medicine [1]. This study investigates the antioxidant potential of Neem flowers using a hydrogen peroxide (H₂O₂) scavenging assay. Extraction was collected by maceration, using the Methanol. Phytochemical screening was done by following schematic procedure and the Antioxidant activity was assessed based on the H2O2 (Hydrogen Peroxide) Free Radical Scavenging Activity Assay method, involving s
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Rouamba, Ablassé, Moussa Compaoré, Maurice Ouédraogo, and Martin Kiendrebeogo. "Human Lymphocyte-Protective Effects of an Ethanol Extract from Detarium microcarpum Guill. and Perr. (Caesalpiniaceae) Fruit Pulp." Antioxidants 7, no. 8 (2018): 104. http://dx.doi.org/10.3390/antiox7080104.

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The current study aimed to evaluate, in vitro, the antioxidant capacity and the human lymphocyte-protective effect of the ethanolic extract from Detarium microcarpum fruit pulp against oxidative stress damage. Human lymphocytes were incubated with different concentrations of extract, followed by the addition of hydrogen peroxide or tert-butyl hydroperoxide. Cell viability was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Furthermore, the antioxidant property of the extract was evaluated in vitro using hydrogen peroxide and nitric oxide radical-scaveng
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Gana, Manjusha K. 1. *. Dr. Ganga Rao B. 2. "SCREENING OF IN VITRO ANTIOXIDANT ACTIVITY OF SELECTED FRUIT PEELS: A COMPARATIVE STUDY." Journal of Pharma Research 7, no. 10 (2018): 227–30. https://doi.org/10.5281/zenodo.1471138.

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<strong><em>ABSTRACT</em></strong> <strong><em>T</em></strong><em>he present was planned to evaluate the in vitro antioxidant activity of selected peel powers such as </em><em>Annona sqaumosa, Actinidia deliciosa, Cucumis melo and Mallus pumila </em><em>using different in vitro assays including the scavenging activities of hydroxyl radical, hydrogen peroxide, Nitric oxide and DPPH radical scavenging activities. The antioxidant activity of peels was compared with standard antioxidant rutin. The comparative study resulted that the Mallus pumila showed better antioxidant activity against the hydr
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Sumi, Salma Akter, Md Afjalus Siraj, Amir Hossain, Md Sagir Mia, Seagufta Afrin, and Md Mustafizur Rahman. "Investigation of the Key Pharmacological Activities of Ficus racemosa and Analysis of Its Major Bioactive Polyphenols by HPLC-DAD." Evidence-Based Complementary and Alternative Medicine 2016 (2016): 1–9. http://dx.doi.org/10.1155/2016/3874516.

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Objective. Oxidative stress leads to numerous physiological disorders including infectious diseases, inflammation, and cancer. The present study was carried out to investigate antioxidant, antibacterial, and cytotoxic activity of methanol crude extract of leaves and fruits of the Ficus racemosa (LCME and FCME, resp.) and to analyse its major bioactive polyphenols by HPLC-DAD. Methods. Antioxidant capacity of the extracts was evaluated by DPPH free radical scavenging, reducing power, total phenolic, total flavonoid, total tannin content assay, superoxide radical, hydroxyl radical, and hydrogen
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Kumar, Ashwani, Vikas Anand, R. C. Dubey, and Kapil K. Goel. "Evaluation of antioxidant potential of alcoholic stem bark extracts of Bauhinia variegata Linn." Journal of Applied and Natural Science 11, no. 1 (2019): 235–39. http://dx.doi.org/10.31018/jans.v11i1.2015.

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The present study was carried out for the evaluation of in-vitro antioxidant potential of alcoholic stem bark (ALSB) extract of Bauhinia variegata Linn. Dried stem bark extract of B. variegata Linn. was screened to evaluate its free radical scavenging effect. Various methods like DPPH assay, reducing power assay, percentage scavenging activity of hydrogen peroxide and superoxide radical scavenging activity were used for screening in-vitro antioxidant potential. Antioxidant potentials were concentration dependent which were compared with standard antioxidants such as butylated hydroxyanisole (B
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Uvarajan, Deenathayalan, and Brindha Durairaj. "Antimicrobial Potential of Zinc Oxide Nanoparticles from Marine Macroalgae." International Journal for Research in Applied Science and Engineering Technology 10, no. 3 (2022): 676–79. http://dx.doi.org/10.22214/ijraset.2022.40608.

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Abstract: The current study evaluates the free antimicrobial and radical scavenging properties of zinc oxide nanoparticles(ZnONP’s) synthesized from an aqueous extract of Sargassum polycystum brown algae. The free radical scavenging potential of ZnO nanoparticles was evaluated through DPPH, Nitric oxide, Hydroxyl radical and Hydrogen peroxide scavenging assays. In addition to that, the antibacterial and antifungal activity of synthesized ZnO nanoparticles was examined against different species. The free radical scavenging and antimicrobial potential of these Sargassum polycystum ZnO nanopartic
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Aitken, Robert J., Alexandra Wilkins, Natasha Harrison, et al. "A Comparative Analysis of the Antioxidant Profiles Generated by the RoXstaTM System for Diverse Biological Fluids Highlights the Powerful Protective Role of Human Seminal Plasma." Antioxidants 14, no. 1 (2025): 90. https://doi.org/10.3390/antiox14010090.

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(1) Background: The RoXstaTM system has been developed as a rapid, effective means of profiling different types of antioxidant activity. The purpose of this study was to examine its performance utilizing a diverse array of biological fluids including semen, blood plasma, serum, urine, saliva, follicular fluid and plant extracts. (2) Methods: The RoXstaTM system was used to assess the ability of different fluids to suppress free radical formation as well as scavenge a variety of toxic oxygen metabolites including free radicals and both hydrogen and organic peroxides. (3) Results: Human semen wa
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Olayemi, ODEKANYIN Oludele, Ayangbemi Abideen Oyinlola, and Jerome Aanuoluwapo Joy. "Enzymatic Production of Sarotherodon galillaeus Muscle Protein Hydrolysates and Assessment of its Alpha-Amylase Inhibitory and Antioxidant Potential." International Journal of Sciences Volume 8, no. 2019-11 (2019): 6–13. https://doi.org/10.5281/zenodo.3979999.

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The study was carried out to evaluate the antioxidant activity and α-amylase activity inhibitory potential of Sarotherodon galillaeus muscle protein hydrolysates. Sarotherodon galillaeus muscle protein isolate was hydrolysed with three digestive proteases namely trypsin, chymotrypsin and pepsin. Degree of hydrolysis was determined. The antioxidative potential of the hydrolysates was investigated using DPPH radical scavenging, ferric reducing power, hydrogen peroxide scavenging and metal chelating activity. The ability of the hydrolysates to inhibit the activity of sugar-hydrolysing enzyme was
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44

U, Swetha. "Antioxidant Activity of Dalbergia Melanoxylon Bark Extract." INTERNATIONAL JOURNAL OF APPLIED PHARMACEUTICAL SCIENCES AND RESEARCH 2, no. 04 (2017): 114–20. http://dx.doi.org/10.21477/ijapsr.v2i04.9904.

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The bark extract of Dalbergia melanoxylon (Fabaceae) was assessed for its antioxidant activity by in-vitro methods. This activity was evaluated by using various methods like hydrogen peroxide scavenging activity, Nitric oxide scavenging activity, DPPH radical scavenging activity and reducing power assay. Quantitative analysis of antioxidative components like total phenolic content, total flavonoid content and antioxidant capacity were estimated using spectrophotometric methods. The obtained results from this method are compared with ascorbic acid acts as a standard antioxidant drug. From the r
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Palaniswamy, Radha, and Palghat Raghunathan Padma. "COMPARISON OF THREE HERBAL PLANTS FOR BEST FREE RADICAL SCAVENGING ACTIVITY." International Journal of Current Pharmaceutical Research 9, no. 3 (2017): 62. http://dx.doi.org/10.22159/ijcpr.2017.v9i3.18898.

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Objective: Excessive production of reactive oxygen species leads to oxidative stress. Antioxidants can prevent or retard the oxidation caused by free radicals and sufficient intake of antioxidants is supposed to protect against diseases. Candidate play Methods: The leaf extracts of these plants were exposed to a battery of free radical scavenging assays like DPPH, ABTS, hydrogen peroxide, superoxide and nitric oxide to determine the free radical scavenging activity.Results: It was observed that the Majoranahortensis leaves in methanolic extract showed maximum scavenging activity.Conclusion: Se
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Ganeshamoorthy, Umakanthan. "Effect of different processing conditions on antioxidant activity of Gracilaria edulis(Rhodophyseae) in Sri Lanka." Journal of Biomedical Engineering and Medical Imaging 6, no. 5 (2019): 19–64. http://dx.doi.org/10.14738/jbemi.65.8007.

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Gracilaria edulis is red marine algae currently cultivated in Sri Lanka but relatively low than other country such as China and Japan. This species are used to produce drugs and healthy delicious low calorie foods because of they are primary source of secondary metabolite and to be used as natural antioxidants and antimicrobials. The Purpose of this research investigates effect of different processing conditions on antioxidant activity of G.edulis (Rhodophyseae) by hydrogen peroxide scavenging activity assay. Absorbance at 230 nm, hydrogen peroxide scavenging assays were done. Different proces
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Vishnuvathan, V. J., K. S. Lakshmi, and A. R. Srividya. "STUDY OF ANTIOXIDANT ACTIVITY OF FORMONONETIN BY IN VITRO METHOD." International Journal of Pharmacy and Pharmaceutical Sciences 9, no. 2 (2017): 273. http://dx.doi.org/10.22159/ijpps.2017v9i2.13964.

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&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;Now the important field of research in phytomedicine is to search a new plant source as well as new phytoconstituents that have fewer side effects and low cost with the free radical scavenging activity&lt;strong&gt;. &lt;/strong&gt;&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods&lt;/strong&gt;:&lt;strong&gt; &lt;/strong&gt;Different &lt;em&gt;in vitro&lt;/em&gt; models such as DPPH, hydroxyl radical, hydrogen peroxide radical, nitric oxide radical and superoxide radical scavenging activity were performed using different concentrations of formononetin r
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Omaji, G. O., M. Osibemhe, B. O. Orji, L. E. Ilouno, B. O. Abdulrahman, and A. O. Ajadi. "Antioxidant Potential of Crude Powder, Methanol and Aqueous Extracts of Fonio Millet (Digitaria exilis) Grains." Nigerian Journal of Biotechnology 38, no. 1 (2021): 91–97. http://dx.doi.org/10.4314/njb.v38i1.11.

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This study evaluated the antioxidant potential along with total phenolic content, total flavonoid content of the crude powder, methanol and aqueous extracts of Digitaria exilis grains. The antioxidant activities of the crude powder and extracts were determined by measuring the reducing ability and hydrogen peroxide scavenging activity. The results showed that methanol extracts exhibited the highest total phenolic content (57.96 ± 6.84 mg gallic acid equivalence/g dried weight) and total flavonoid content (38.75 ± 9.76 mg quercetin equivalence/g dried weight) compared to the crude powder and aq
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49

SASIDHARAN, M. "IN VITRO ANALYSIS OF ANTIOXIDANT ACTIVITY IN POLYHERBAL PLANT EXTRACT." YMER Digital 21, no. 05 (2022): 1499–514. http://dx.doi.org/10.37896/ymer21.05/g4.

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Most plants are considered to prevent free radicals associated damages by numerous ways including direct scavenging of free radicals and inhibition of enzymes involved in free radical production. The aim of the present study was to examine the antioxidant activities of the hexane from polyherbal plants of Carica papaya, Punica granatum, Catharanthus roseus and Cympbopogan citratus which is long been known to be a very important source of pharmaceutical. Different concentrations of polyherbal extract was tested for anti-oxidant properties using hydrogen peroxide assay. Similarly, the free radic
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50

Ilechukwu, C. C., C. O. Ezenwelu, J. C. Ifemeje, et al. "Comparative In vitro Antioxidant Studies and Phytochemical Analysis of Ginger (Zingiber officinale) and Garlic (Allium sativum)." IPS Journal of Nutrition and Food Science 4, no. 3 (2025): 488–93. https://doi.org/10.54117/ijnfs.v4i3.110.

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Ginger (Zingiber officinale) and garlic (Allium sativum) are globally consumed spices known for their flavor, organoleptic properties, and health benefits. This study evaluates the comparative in-vitro antioxidant and phytochemical profiles of Zingiber officinale and Allium sativum. Fresh samples were washed, chopped, dried, pulverized, and extracted using standard procedures. Phytochemical composition and antioxidant parameters (hydrogen peroxide scavenging and ferric reducing antioxidant property, FRAP) of the methanol extracts were assessed. Phytochemical screening revealed the presence of
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