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Journal articles on the topic 'Hydrophilic Interaction Liquid Chromatoghraphy (HILIC)'

Author: Grafiati

Published: 20 February 2023

Last updated: 31 July 2025

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1

Ajay, A.* Prasanth S. S. Sanooja P. k. Jisha U. K. T. Akshara Riya Rajan Sibina M. K. Mohammed Farooq P. "Hydrophilic Interaction Liquid Chromatography (Hilic)." International Journal of Pharmaceutical Sciences 2, no. 8 (2024): 2816–21. https://doi.org/10.5281/zenodo.13292739.

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Hydrophilic interaction liquid chromatography or HILIC is a powerful analytical technique for the separation and analysis of polar and hydrophilic compounds, including small molecules, peptides, and proteins. HILIC is a variation of normal phase chromatography. It is also called as “Reverse reversed-phase” or “Aqueous normal-phase” chromatography. This chromatographic method capitalizes on the unique interactions between analytes and a polar stationary phase, which enhances the retention of hydrophilic compounds through mechanisms such as partitioning, hydrogen bonding

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2

Boersema, Paul J., Shabaz Mohammed, and Albert J. R. Heck. "Hydrophilic interaction liquid chromatography (HILIC) in proteomics." Analytical and Bioanalytical Chemistry 391, no. 1 (2008): 151–59. http://dx.doi.org/10.1007/s00216-008-1865-7.

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3

Noga, Sylwia, Attila Felinger, and Bogusław Buszewski. "Hydrophilic Interaction Liquid Chromatography and Per Aqueous Liquid Chromatography in Fungicides Analysis." Journal of AOAC INTERNATIONAL 95, no. 5 (2012): 1362–70. http://dx.doi.org/10.5740/jaoacint.sge_noga.

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Abstract The goal of the study was to investigate the retention mechanism of selected fungicides in hydrophilic interaction liquid chromatography (HILIC) and per aqueous liquid chromatography (PALC). Chromatographic measurements were made on four physicochemically diversified HILIC columns, which were evaluated for the analysis of nine biologically active compounds, such as strobilurins and triazoles. The effects of the operating conditions on separations were investigated, including the concentration of the organic solvent in the aqueous-organic (acetonitrile) mobile phase. The results were c

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Radoičić, Aleksandra, Sandra Šegan, Aleksandra Dramićanin, and Dušanka Milojković-Opsenica. "Hydrophilic Interaction Liquid Chromatography for the Analysis of Pharmaceutical Formulations." Current Analytical Chemistry 20, no. 5 (2024): 295–317. http://dx.doi.org/10.2174/0115734110290557240305045032.

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Abstract: For a long time, Reversed-Phase Liquid Chromatography (RPLC) was the most dominant technique for the analysis of pharmaceutical compounds, but with poor efficiency in the separation of small polar molecules. From the efforts to solve the problem of insufficient retention of these molecules, during the last decades, a mode of liquid chromatography named Hydrophilic Interaction Liquid Chromatography (HILIC) has experienced vast expansion. It is based on the use of a highly hydrophilic stationary phase along with an aqueous mobile phase with high organic modifier content. In this review

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5

Skoczylas, Magdalena, Szymon Bocian, and Bogusław Buszewski. "Dipeptide-bonded stationary phases for hydrophilic interaction liquid chromatography." RSC Advances 6, no. 98 (2016): 96389–97. http://dx.doi.org/10.1039/c6ra17704b.

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6

Wang, Meng, Ryoichi Hiraki, Norihiro Nakamura, et al. "Determination of Gemcitabine in Plasma of Bladder Cancer Patients by Hydrophilic Interaction Chromatography with Ultraviolet Detection." Journal of Chromatographic Science 58, no. 7 (2020): 587–90. http://dx.doi.org/10.1093/chromsci/bmaa020.

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Abstract Gemcitabine is a deoxycytidine analog that has been used for a broad spectrum of tumor, such as nonsmall-cell lung cancer, bladder cancer and pancreatic cancer. Because gemcitabine is hydrophilic, hydrophilic interaction liquid chromatography (HILIC), where analytes are retained on a polar column according to their hydrophilicity, should be adequate for separation analysis of gemcitabine. In the present study, we proposed a hydrophilic interaction chromatography with ultraviolet (HILIC-UV) method with liquid–liquid extraction and adding tetrahydrouridine to plasma samples for gemcitab

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Sentkowska, Aleksandra, Magdalena Biesaga, and Krystyna Pyrzynska. "Application of Hydrophilic Interaction Liquid Chromatography for the Quantification of Flavonoids inGenista tinctoriaExtract." Journal of Analytical Methods in Chemistry 2016 (2016): 1–9. http://dx.doi.org/10.1155/2016/3789348.

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Hydrophilic interaction chromatography (HILIC) was employed to investigate chromatographic behavior of selected flavonoids from their different subgroups differing in polarity. Chromatographic measurements were performed on two different HILIC columns: unmodified silica (Atlantis-HILIC) and zwitterionic sulfoalkylbetaine (SeQuant ZIC-HILIC). Separation parameters such as content and type of organic modifier were studied. On ZIC column retention factors were observed to be inversely proportional to the buffer content in the mobile phase, which is the typical partitioning mechanism. In the case

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Si, Tiantian, Licheng Wang, Xiaofeng Lu, Xiaojing Liang, Shuai Wang, and Yong Guo. "An alternative approach for the preparation of a core–shell bimetallic central metal–organic framework as a hydrophilic interaction liquid chromatography stationary phase." Analyst 145, no. 11 (2020): 3851–56. http://dx.doi.org/10.1039/d0an00304b.

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Wang, Yadi, M. Farooq Wahab, Zachary S. Breitbach, and Daniel W. Armstrong. "Carboxylated cyclofructan 6 as a hydrolytically stable high efficiency stationary phase for hydrophilic interaction liquid chromatography and mixed mode separations." Analytical Methods 8, no. 31 (2016): 6038–45. http://dx.doi.org/10.1039/c6ay01246a.

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10

Ortiz-Villanueva, Elena, Meritxell Navarro-Reig, Joaquim Jaumot, and Romà Tauler. "Chemometric evaluation of hydrophilic interaction liquid chromatography stationary phases: resolving complex mixtures of metabolites." Analytical Methods 9, no. 5 (2017): 774–85. http://dx.doi.org/10.1039/c6ay02976k.

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11

Frensemeier, Lisa M., Lars Büter, Martin Vogel, and Uwe Karst. "Investigation of the oxidative transformation of roxarsone by electrochemistry coupled to hydrophilic interaction liquid chromatography/mass spectrometry." Journal of Analytical Atomic Spectrometry 32, no. 1 (2017): 153–61. http://dx.doi.org/10.1039/c6ja00277c.

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12

Buszewski, Bogusław, and Sylwia Noga. "Hydrophilic interaction liquid chromatography (HILIC)—a powerful separation technique." Analytical and Bioanalytical Chemistry 402, no. 1 (2011): 231–47. http://dx.doi.org/10.1007/s00216-011-5308-5.

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13

Andrade-Eiroa, Auréa, Roya Shahla, Manolis N. Romanías, and Philippe Dagaut. "An alternative to trial and error methodology in solid phase extraction: an original automated solid phase extraction procedure for analysing PAHs and PAH-derivatives in soot." RSC Adv. 4, no. 63 (2014): 33636–44. http://dx.doi.org/10.1039/c4ra03214d.

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This paper introduces the generalization of reverse-phase HPLC fundamentals to normal-phase liquid chromatography, hydrophilic interaction chromatography (HILIC) and Automated Solid Phase Extraction (A-SPE).

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Wu, Shengjie, Xiuling Li, Feifang Zhang, Ge Jiang, Xinmiao Liang, and Bingcheng Yang. "An arginine-functionalized stationary phase for hydrophilic interaction liquid chromatography." Analyst 140, no. 12 (2015): 3921–24. http://dx.doi.org/10.1039/c5an00570a.

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A arginine-functionalized HILIC stationary phase has been prepared for the first time by clicking arginine onto silica gel. As an HPLC column, it offered the efficient separation of highly polar analytes. As a SPE sorbent, it exhibited excellent selectivity and enrichment toward acidic glycopeptides.

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Guarducci, Maria A., Andrea Fochetti, Alessia Ciogli, and Giulia Mazzoccanti. "A Compendium of the Principal Stationary Phases Used in Hydrophilic Interaction Chromatography: Where Have We Arrived?" Separations 10, no. 1 (2022): 22. http://dx.doi.org/10.3390/separations10010022.

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Hydrophilic interaction liquid chromatography (HILIC) today is a well-known and largely applied technique to analyse polar compounds such as pharmaceuticals, metabolites, proteins, peptides, amino acids, oligonucleotides, and carbohydrates. Due to the large number of stationary phases employed for HILIC applications, this review aims to help the reader in choosing a proper stationary phase, which often represents the critical point for the success of a separation. A great offer is present for achiral applications in contrast to the chiral phases developed for HILIC enantioseparations. In the l

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Liu, Houmei, Yong Guo, Xusheng Wang, Xiaojing Liang, and Xia Liu. "Amino-terminated ionic liquid modified graphene oxide coated silica composite stationary phase for hydrophilic interaction chromatography." RSC Adv. 4, no. 70 (2014): 37381–88. http://dx.doi.org/10.1039/c4ra03432e.

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17

Cajka, Tomas, Jiri Hricko, Stanislava Rakusanova, et al. "Hydrophilic Interaction Liquid Chromatography–Hydrogen/Deuterium Exchange–Mass Spectrometry (HILIC-HDX-MS) for Untargeted Metabolomics." International Journal of Molecular Sciences 25, no. 5 (2024): 2899. http://dx.doi.org/10.3390/ijms25052899.

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Liquid chromatography with mass spectrometry (LC-MS)-based metabolomics detects thousands of molecular features (retention time–m/z pairs) in biological samples per analysis, yet the metabolite annotation rate remains low, with 90% of signals classified as unknowns. To enhance the metabolite annotation rates, researchers employ tandem mass spectral libraries and challenging in silico fragmentation software. Hydrogen/deuterium exchange mass spectrometry (HDX-MS) may offer an additional layer of structural information in untargeted metabolomics, especially for identifying specific unidentified m

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18

Jiménez, Gabriel Marcelín. "Book Review: Hydrophilic Interaction Liquid Chromatography (HILIC) and Advanced Applications." Bioanalysis 3, no. 15 (2011): 1687–88. http://dx.doi.org/10.4155/bio.11.160.

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19

Nakov, Natalija, Rumenka Petkovska, Liljana Ugrinova, Suzana Trajkovic-Jolevska, and Aneta Dimitrovska. "Determination of Rocuronium bromide by hydrophilic interaction liquid chromatography (HILIC)." Macedonian Pharmaceutical Bulletin 57 (2001): 17–24. http://dx.doi.org/10.33320/maced.pharm.bull.2011.57.002.

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A new method involving hydrophilic interaction liquid chromatography (HILIC) has been developed for determination of rocuronium bromide in presents of its main impurities (impurity A and impurity C), which are also its main degradation products, in solution for injection. The influence of the critical chromatographic parameters such as content of acetonitrile in the mobile phase, ionic strength and pH value of the buffer used in the mobile phase were investigated using the Design of experiments approach (DoE). The mechanism of retention of rocuronium bromide on bare silica column was also inve

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20

Peris-García, Ester, Raquel Burgos-Gil, María Celia García-Alvarez-Coque, and María José Ruiz-Angel. "Hydrophilic Liquid Chromatography versus Reversed-Phase Liquid Chromatography in the Absence and the Presence of 1-Hexyl-3-methylimidazolium Chloride for the Analysis of Basic Compounds." Separations 7, no. 2 (2020): 30. http://dx.doi.org/10.3390/separations7020030.

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In reversed-phase liquid chromatography (RPLC), positively charged basic compounds yield broad and asymmetric peaks, as a result of ionic interactions with free silanols that remain on conventional silica-based columns. Diverse solutions have been proposed to mask the silanophilic activity, which is translated to an improved peak shape. In this work, the chromatographic performance of hydrophilic interaction liquid chromatography (HILIC) was evaluated as an alternative to the addition of an ionic liquid (IL) to the aqueous-organic mobile phase used with RPLC columns, for the analysis of eight

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21

Redón, Lídia, Xavier Subirats, and Martí Rosés. "Evaluation of Hold-Up Volume Determination Methods and Markers in Hydrophilic Interaction Liquid Chromatography." Molecules 28, no. 3 (2023): 1372. http://dx.doi.org/10.3390/molecules28031372.

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Common methods for hold-up time and volume determination in Reversed-Phase Liquid Chromatography (RPLC) have been tested for Hydrophilic Interaction Liquid Chromatography (HILIC). A zwitterionic ZIC-HILIC column has been used for the testing. The pycnometric determination method, based on differences in column weight when filled with water or organic solvent, provides the overall volume of solvent inside the column. This includes the volume of eluent semi-sorbed on the packing of the column, which acts as the main stationary phase. The homologous series approach, based on the retention behavio

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Kozlik, Petr, Katarina Molnarova, Tomas Jecmen, Tomas Krizek, and Zuzana Bosakova. "Prediction of Intact N-Glycopeptide Retention Time Windows in Hydrophilic Interaction Liquid Chromatography." Molecules 27, no. 12 (2022): 3723. http://dx.doi.org/10.3390/molecules27123723.

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Analysis of protein glycosylation is challenging due to micro- and macro-heterogeneity of the attached glycans. Hydrophilic interaction liquid chromatography (HILIC) is a mode of choice for separation of intact glycopeptides, which are inadequately resolved by reversed phase chromatography. In this work, we propose an easy-to-use model to predict retention time windows of glycopeptides in HILIC. We constructed this model based on the parameters derived from chromatographic separation of six differently glycosylated peptides obtained from tryptic digests of three plasma proteins: haptoglobin, h

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Struwe, Weston B., and Pauline M. Rudd. "Aminoquinolines as fluorescent labels for hydrophilic interaction liquid chromatography of oligosaccharides." Biological Chemistry 393, no. 8 (2012): 757–65. http://dx.doi.org/10.1515/hsz-2012-0169.

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Abstract In this study, we investigated the potential of four different aminoquinoline (AQ) compounds as fluorescent labels for glycan analysis using hydrophilic interaction liquid chromatography (HILIC) and fluorescence detection (FLD). We confirmed the optimal excitation and emission wavelengths of 3-AQ and 6-AQ conjugated to glycan standards using three-dimensional fluorescent spectral scanning. The optimal excitation and emission wavelengths for 6-AQ were confirmed at λex=355 nm and λem=440 nm. We concluded that the optimal wavelengths for 3-AQ were λex=355 nm and λem=420 nm, which differe

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Jovanović, Marko, Biljana Stojanović, Tijana Rakić, Anđelija Malenović, Darko Ivanović, and Mirjana Medenica. "Five different columns in the analysis of basic drugs in hydrophilic interaction liquid chromatography." Open Chemistry 11, no. 7 (2013): 1150–62. http://dx.doi.org/10.2478/s11532-013-0245-0.

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AbstractFive different columns (two silica, two cyanopropyl and one diol) were investigated in hydrophilic interaction liquid chromatography (HILIC). For the assessment of columns behavior in HILIC mode, six basic drugs (lamotrigine, thioridazine, clozapine, chlorpheniramine, pheniramine and sulpiride) were chosen. The assessment of the influence of the concentration of organic modifier on analytes’ retention on each column was provided by fitting the retention data into theoretical models. Utilizing the statistical analysis, the selection of the model that provides better prediction of the re

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Maksic, Jelena, Anja Tumpa, Igor Popovic, and Biljana Jancic-Stojanovic. "Investigation of olopatadine hydrochloride under stress conditions by hydrophilic interaction liquid chromatography." Chemical Industry 70, no. 3 (2016): 339–46. http://dx.doi.org/10.2298/hemind150404039m.

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The purpose of the present research was to conduct stress degradation studies on the olopatadine hydrochloride, an antiallergic drug, using the hydrophilic interaction liquid chromatography (HILIC). HILIC requires the utilization of polar and moderately polar stationary phases and aqueous-organic mobile phase usually containing more than 70% of organic solvent. In this study, olopatadine hydrochloride was subjected to acid and base hydrolysis, oxidation and termolytic degradation in order to estimate its stability under different stress conditions recommended by ICHQ1A (R2) guideline. Degree o

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Zauner, Gerhild, André M. Deelder, and Manfred Wuhrer. "Recent advances in hydrophilic interaction liquid chromatography (HILIC) for structural glycomics." ELECTROPHORESIS 32, no. 24 (2011): 3456–66. http://dx.doi.org/10.1002/elps.201100247.

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27

Peris-García, Ester, María José Ruiz-Angel, Juan José Baeza-Baeza, and María Celia García-Alvarez-Coque. "Comparison of the Fitting Performance of Retention Models and Elution Strength Behaviour in Hydrophilic-Interaction and Reversed-Phase Liquid Chromatography." Separations 8, no. 4 (2021): 54. http://dx.doi.org/10.3390/separations8040054.

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Hydrophilic interaction liquid chromatography (HILIC) is able to separate from polar to highly polar solutes, using similar eluents to those in the reversed-phase mode (RPLC) and a polar stationary phase, where water is adsorbed onto its surface. It is widely accepted that multiple modes of interaction take place in the HILIC environment, which can be far more complex than the interactions in an RPLC column. The behaviour in HILIC should be adequately modelled to predict the retention with optimisation purposes and improve the understanding on retention mechanisms, as is the case for RPLC. In

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Raikou, Vasiliki, Eleni Kalogria, Athanasia Varvaresou, Efthimios Tsirivas, and Irene Panderi. "Quantitation of Acetyl Hexapeptide-8 in Cosmetics by Hydrophilic Interaction Liquid Chromatography Coupled to Photo Diode Array Detection." Separations 8, no. 8 (2021): 125. http://dx.doi.org/10.3390/separations8080125.

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Bioactive peptides are gaining more and more popularity in the research and development of cosmetic products with anti-aging effect. Acetyl hexapeptide-8 is a hydrophilic peptide incorporated in cosmetics to reduce the under-eye wrinkles and the forehead furrows. Hydrophilic interaction liquid chromatography (HILIC) is the separation technique of choice for analyzing peptides. In this work, a rapid HILIC method coupled to photodiode array detection operated at 214 nm was developed, validated and used to determine acetyl-hexapeptide-8 in cosmetics. Chromatography was performed on a Xbridge® HIL

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Yan, Kui, Haiyan Yang, Shaohua Huang, and Zhengwu Bai. "A sulfonated chitooligosaccharide modified silica material for hydrophilic interaction liquid chromatography and its chromatographic evaluation." Analytical Methods 10, no. 10 (2018): 1258–65. http://dx.doi.org/10.1039/c8ay00070k.

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Galeano Garcia, Zimmermann, and Carazzone. "Hydrophilic Interaction Liquid Chromatography Coupled to Mass Spectrometry and Multivariate Analysis of the De Novo Pyrimidine Pathway Metabolites." Biomolecules 9, no. 8 (2019): 328. http://dx.doi.org/10.3390/biom9080328.

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In this study, we describe the optimization of a Hydrophilic Interaction Liquid Chromatography coupled to mass spectrometry (HILIC-MS) method for the evaluation of 14 metabolites related to the de novo synthesis of pyrimidines (dnSP) while using multivariate analysis, which is the metabolic pathway for pyrimidine nucleotide production. A multivariate design was used to set the conditions of the column temperature, flow of the mobile phase, additive concentration, gradient rate, and pH of the mobile phase in order to attain higher peak resolution and ionization efficiency in shorter analysis ti

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Peng, Yahui, Feifang Zhang, Xiao Pan, Yanjie Hou, and Bingcheng Yang. "Poly(vinyl alcohol)–cationic cellulose copolymer encapsulated SiO2 stationary phase for hydrophilic interaction liquid chromatography." RSC Advances 7, no. 34 (2017): 21336–41. http://dx.doi.org/10.1039/c7ra01958k.

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32

Louis, Alexandra, Jean François Chich, Hadrien Chepca, Isabelle Schmitz, Philippe Hugueney, and Alessandra Maia-Grondard. "Green Extraction Method: Microwave-Assisted Water Extraction Followed by HILIC-HRMS Analysis to Quantify Hydrophilic Compounds in Plants." Metabolites 15, no. 4 (2025): 223. https://doi.org/10.3390/metabo15040223.

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Background: Hydrophilic compounds, such as amino acids, organic acids and sugars, among others, are present in large amounts in plant cells. The analysis and quantification of these major hydrophilic compounds are particularly relevant in plant science because they have a considerable impact on the quality of plant-derived products and on plant–pathogen relationships. Our objective was to develop and validate a complete analysis workflow combining a water-based extraction procedure with a fast separation using hydrophilic interaction liquid chromatography coupled to high-resolution mass spectr

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Alsaeedi, Majidah, Huda Alghamdi, Phyllis Hayes, Anna Hogan, and Jeremy Glennon. "Efficient Sub-1 Minute Analysis of Selected Biomarker Catecholamines by Core-Shell Hydrophilic Interaction Liquid Chromatography (HILIC) with Nanomolar Detection at a Boron-Doped Diamond (BDD) Electrode." Separations 8, no. 8 (2021): 124. http://dx.doi.org/10.3390/separations8080124.

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A rapid, sensitive method for the separation of catecholamine biomarkers (CAs), of importance in traumatic brain injury (TBI) and in Parkinson’s disease (PD), has been successfully developed using hydrophilic interaction liquid chromatography (HILIC). Dopamine (DA), epinephrine (EPI), and norepinephrine (NE) are known to be three to fivefold elevated above normal in traumatic brain injury (TBI) patients. HILIC facilitates the rapid and efficient separation of these polar biomarkers, which can be poorly retained by reversed-phase liquid chromatography (RPLC), while electrochemical detection (EC

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Tripathy, Swarnajeet, Anindya Bose, Prafulla Kumar Sahu, et al. "Simultaneous Estimation of Levodopa and Carbidopa in Mice Plasma by LC-MS/MS." Asian Journal of Chemistry 34, no. 6 (2022): 1587–91. http://dx.doi.org/10.14233/ajchem.2022.23720.

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A simple and sensitive liquid chromatographic method along with tandem mass detection has been developed for the determination of levodopa and carbidopa in mice plasma. Owing the hydrophilic nature of both the analytes, a hydrophilic interaction liquid chromatography (HILIC) setup was used for their separation by a Merck (Germany) HILIC column (4.6 × 250 mm, 5 μm; 200 Å). The mobile phase composed equal proportion of water and acetonitrile both containing 0.1% formic acid at a flow rate of 1.4 mL/min to achieve rapid separation of the compounds. The column was coupled with a triple quadrupole

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Panderi, Irene, Eugenia Taxiarchi, Constantinos Pistos, Eleni Kalogria, and Ariadni Vonaparti. "Insights into the Mechanism of Separation of Bisphosphonates by Zwitterionic Hydrophilic Interaction Liquid Chromatography: Application to the Quantitation of Risedronate in Pharmaceuticals." Separations 6, no. 1 (2019): 6. http://dx.doi.org/10.3390/separations6010006.

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Bisphosphonates are used to treat various skeletal disorders, as they modulate bone metabolism by inhibition of the osteoclast-mediated bone resorption. These compounds are both polar and ionic, and therefore, by using reversed phase liquid chromatography are eluted rapidly. Hydrophilic interaction liquid chromatography (HILIC) is an advantageous technique for the separation and analysis of polar molecules. As the elution order in HILIC is reversed to reversed phase liquid chromatography, a reasonable retention and selectivity for polar compounds is expected. In this work the retention mechani

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Molnarova, Katarina, and Petr Kozlík. "Comparison of Different HILIC Stationary Phases in the Separation of Hemopexin and Immunoglobulin G Glycopeptides and Their Isomers." Molecules 25, no. 20 (2020): 4655. http://dx.doi.org/10.3390/molecules25204655.

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Protein glycosylation analysis is challenging due to the structural variety of complex conjugates. However, chromatographically separating glycans attached to tryptic peptides enables their site-specific characterization. For this purpose, we have shown the importance of selecting a suitable hydrophilic interaction liquid chromatography (HILIC) stationary phase in the separation of glycopeptides and their isomers. Three different HILIC stationary phases, i.e., HALO® penta-HILIC, Glycan ethylene bridged hybrid (BEH) Amide, and ZIC-HILIC, were compared in the separation of complex N-glycopeptide

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Turrell, Elizabeth, Lesley Stobo, Jean-Pierre Lacaze, Sergey Piletsky, and Elena Piletska. "Optimization of Hydrophilic Interaction Liquid Chromatography/Mass Spectrometry and Development of Solid-Phase Extraction for the Determination of Paralytic Shellfish Poisoning Toxins." Journal of AOAC INTERNATIONAL 91, no. 6 (2008): 1372–86. http://dx.doi.org/10.1093/jaoac/91.6.1372.

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Abstract The combination of hydrophilic interaction liquid chromatography (HILIC) and liquid chromatography/mass spectrometry (LC/MS) for the determination of paralytic shellfish poisoning (PSP) toxins has been proposed for use in routine monitoring of shellfish. In this study, methods for the detection of multiple PSP toxins [saxitoxin (STX), neosaxitoxin (NEO), decarbamoyl saxitoxin (dcSTX), decarbamoyl neosaxitoxin (dcNEO), gonyautoxins 15 (GTX1, GTX2, GTX3, GTX4, GTX5), decarbamoyl gonyautoxins (dcGTX2 and dcGTX3), and the N-sulfocarbamoyl C toxins (C1 and C2)] were optimized using single

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Rajendiran, Vaithilingam, and Ziad El Rassi. "Silica-Based Stationary Phase with Surface Bound N-Acetyl-glucosamine for Hydrophilic Interaction Liquid Chromatography." Molecules 28, no. 20 (2023): 7099. http://dx.doi.org/10.3390/molecules28207099.

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A hydrophilic silica-based stationary phase with surface bound N-acetylglucosamine (GlcNAc-silica) was prepared in house and characterized physically via Fourier transform infrared (FTIR) analysis and thermogravimetric analysis (TGA) and chromatographically over a wide range of mobile phase compositions. While both FTIR and TGA confirmed the attachment of the GlcNAc ligands to the silica surface, the chromatographic evaluation of GlcNAc-silica with polar and slightly polar standard solutes (e.g., sugars, nucleic acid fragments, phenolic, and benzoic acid derivatives) yielded the typical hydrop

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Fan, Zi-qiang, Hong Yu, and Ya-qi Cai. "Determination of alkyl ammonium ionic liquid cations by hydrophilic interaction liquid chromatography and its application in analysis of environmental water." Analytical Methods 10, no. 24 (2018): 2812–20. http://dx.doi.org/10.1039/c8ay00720a.

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Analysis of alkyl ammonium ionic liquid cations in environmental water by the reversed phase ion-pair SPE combined with HILIC-IUV. (a) The chromatogram of alkyl ammonium cations; (b) the chromatogram of alkyl ammonium cations after enrichment.

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Chapel, Soraya, Florent Rouvière, Davy Guillarme, and Sabine Heinisch. "Reversed HILIC Gradient: A Powerful Strategy for On-Line Comprehensive 2D-LC." Molecules 28, no. 9 (2023): 3907. http://dx.doi.org/10.3390/molecules28093907.

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The aim of the present work is to evaluate the possibilities and limitations of reversed hydrophilic interaction chromatography (revHILIC) mode in liquid chromatography (LC). This chromatographic mode consists of combining a highly polar stationary phase (bare silica) with a gradient varying from very low (1–5%) to high (40%) acetonitrile content (reversed gradient compared to HILIC). The retention behavior of revHILIC was first compared with that of reversed-phase LC (RPLC) and HILIC using representative mixtures of peptides and pharmaceutical compounds. It appears that the achievable selecti

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Al-Aphalahy, Bashaer A., Ameen W. Qassim, and Raad R. Karabat. "Knowledge of The HILIC Retention Behaviors of Two Quinolone Antibiotics on Sulfobetaine-Type Zwitterionic Stationary Phases." INTERNATIONAL JOURNAL OF DRUG DELIVERY TECHNOLOGY 13, no. 01 (2023): 372–75. http://dx.doi.org/10.25258/ijddt.13.1.60.

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Fluoroquinolones treat infections of the urinary, respiratory, gastrointestinal, skin, bone, and joint systems. This article describes the development of the hydrophilic interaction liquid chromatography (ZIC-HILIC) method to determine norfl oxacin and ofl oxacin retention characteristics. Separation of all analytes was accomplished using ZIC-HILIC1 and ZIC-HILIC4 columns. The mobile phase was 40 mM sodium acetate water solution in acetonitrile pumped at a 0.7 mL/min fl ow rate. The infl uence of acetonitrile concentration, the presence of salt in the mobile phase, and pH on the retention of n

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Qian, Ziqi, Yi Wang, Shuyu Shen, et al. "Analysis of Proanthocyanidins in Plant Materials Using Hydrophilic Interaction HPLC-QTOF-MS." Molecules 27, no. 9 (2022): 2684. http://dx.doi.org/10.3390/molecules27092684.

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Proanthocyanidins (PACs) have been proven to possess a wide range of biological activities, but complex structures limit their study of structure–function relationships. Therefore, an efficient and general method using hydrophilic interaction high-performance liquid chromatography coupled with high-resolution quadrupole time-of-flight tandem mass spectrometry (HILIC-QTOF-MS) was established to analyze PACs from different plant materials. This method was successfully applied to characterize PACs from Chinese bayberry (Myrica rubra Sieb. et Zucc.) leaves (BLPs), sorghum testa (STPs) and grape se

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Nakov, Natalija, Jelena Acevska, Katerina Brezovska, Rumenka Petkovska, and Aneta Dimitrovska. "Optimization of hydrophilic interaction liquid chromatographic method for simultaneous determination of cetylpyridinium chloride and benzocaine in lozenges." Macedonian Journal of Chemistry and Chemical Engineering 31, no. 1 (2012): 47. http://dx.doi.org/10.20450/mjcce.2012.56.

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A new Hydrophilic Interaction Liquid Chromatography (HILIC) method was developed for simultaneous determination of cetylpyridinium chloride and benzocaine as active substances in lozenges. Method development included investigation of the influence of the critical chromatographic conditions such as: type of organic modifier, mobile phase strength, ionic strength and pH value of the buffer used in the mobile phase, as well as experimental determination of dominant mechanism of retention. The optimal chromatographic conditions were obtained using bare silica column and a mobile phase consisting o

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Al-Tannak, Naser F., Sami Bawazeer, and David G. Watson. "Exploring the Effect of Buffer Strength on the Retention Time of Weak Acids, Neutral and Weak Bases in Hydrophilic Interaction Liquid Chromatography (HILIC) Mode." Current Analytical Chemistry 15, no. 1 (2018): 34–46. http://dx.doi.org/10.2174/1573411014666180806152818.

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Background: Hydrophilic Interaction Liquid Chromatography (HILIC) orthogonal to conventional reversed phase High-Performance Liquid Chromatography (HPLC) mode allowing separation of polar compounds. HILIC has been reported to be an alternative to normal phase liquid chromatography, yet the separation mechanism reported in HILIC is much more complicated than that in normal phase liquid chromatography. Objective: To investigate the effect of water layer thickness on silica gel and the amount of ammonium ions present within the buffer on retention mechanism in hydrophilic interaction chromatograp

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Fical, Luboš, Maria Khalikova, Hana Kočová Vlčková, et al. "Determination of Antiviral Drugs and Their Metabolites Using Micro-Solid Phase Extraction and UHPLC-MS/MS in Reversed-Phase and Hydrophilic Interaction Chromatography Modes." Molecules 26, no. 8 (2021): 2123. http://dx.doi.org/10.3390/molecules26082123.

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Two new ultra-high performance liquid chromatography (UHPLC) methods for analyzing 21 selected antivirals and their metabolites were optimized, including sample preparation step, LC separation conditions, and tandem mass spectrometry detection. Micro-solid phase extraction in pipette tips was used to extract antivirals from the biological material of Hanks balanced salt medium of pH 7.4 and 6.5. These media were used in experiments to evaluate the membrane transport of antiviral drugs. Challenging diversity of physicochemical properties was overcome using combined sorbent composed of C18 and i

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Wang, Qian, Yue Yuan, Mingxin Li, et al. "Application of solvent demulsification dispersive liquid–liquid microextraction for nicotine and cotinine measurement in human urine combined with hydrophilic interaction chromatography tandem mass spectrometry." Analytical Methods 9, no. 29 (2017): 4311–18. http://dx.doi.org/10.1039/c7ay00840f.

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Acevska, Jelena, Gjoshe Stefkov, Natalija Nakov, et al. "Chemometric approach for development, optimization and validation of HILIC methods used for the determination of alkaloids from poppy straw." Macedonian Journal of Chemistry and Chemical Engineering 33, no. 1 (2014): 73. http://dx.doi.org/10.20450/mjcce.2014.6.

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<p class="abstract">Two hydrophilic interaction liquid chromatography (HILIC) methods for simultaneous determination of the major alkaloids (morphine, codeine, thebaine, oripavine, papaverine and noscapine) in opium poppy straw (<em>Papaver somniferum</em> L., Papaveraceae) were developed.</p><p class="abstract">The chromatographic behavior of target alkaloids was investigated on two different HILIC stationary phases: bare silica (Si-HILIC) and zwitterionic (ZIC-HILIC). Systematic optimization of the methods was carried out by use of chemometrics. A full factorial

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Sommella, Eduardo, Emanuela Salviati, Simona Musella, Veronica Di Sarno, Francesco Gasparrini, and Pietro Campiglia. "Comparison of Online Comprehensive HILIC × RP and RP × RP with Trapping Modulation Coupled to Mass Spectrometry for Microalgae Peptidomics." Separations 7, no. 2 (2020): 25. http://dx.doi.org/10.3390/separations7020025.

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In this work, two online comprehensive two-dimensional liquid chromatography platforms, namely Hydrophilic interaction liquid chromatography × Reversed phase (HILIC × RP) and Reversed phase × Reversed Phase (RP × RP) coupled to mass spectrometry, were compared for the analysis of complex peptide samples. In the first dimension, a HILIC Amide and C18 Bioshell peptide (150 × 2.1 mm, 1.7 and 2.0 μm) columns were selected, while, in the second dimension, a short C18 (50 × 3.0 mm, 2.7 μm) Bioshell peptide column was used. Two C18 trapping columns (10 × 3.0 mm, 1.9 μm), characterized by high retenti

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Sun, Yongming, Biqi Xia, Xiangzhun Chen, Chuansong Duanmu, Denghao Li, and Chao Han. "Rapid Quantification of Four Anthocyanins in Red Grape Wine by Hydrophilic Interaction Liquid Chromatography/ Triple Quadrupole Linear Ion Trap Mass Spectrometry." Journal of AOAC INTERNATIONAL 98, no. 6 (2015): 1628–31. http://dx.doi.org/10.5740/jaoacint.15-098.

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Abstract The identification and quantification of four anthocyanins (cyanidin-3-O -glucoside, peonidin- 3-O -glucoside, delphinidin-3-O -glucoside, and malvidin-3-O-glucoside) in red grape wine were carried out by hydrophilic interaction liquid chromatography/triple quadrupole linear ion trap MS (HILIC/QTrap-MS/MS). Samples were diluted directly and separated on a Merck ZIC HILIC column with 20 mM ammonium acetate solution–acetonitrile mobile phase. Quantitative data acquisition was carried out in the multiple reaction monitoring mode. Additional identification and confirmation of target compo

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Hosseinkhani, Farideh, Luojiao Huang, Anne-Charlotte Dubbelman, Faisa Guled, Amy C. Harms, and Thomas Hankemeier. "Systematic Evaluation of HILIC Stationary Phases for Global Metabolomics of Human Plasma." Metabolites 12, no. 2 (2022): 165. http://dx.doi.org/10.3390/metabo12020165.

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Polar hydrophilic metabolites have been identified as important actors in many biochemical pathways. Despite continuous improvement and refinement of hydrophilic interaction liquid chromatography (HILIC) platforms, its application in global polar metabolomics has been underutilized. In this study, we aimed to systematically evaluate polar stationary phases for untargeted metabolomics by using HILIC columns (neutral and zwitterionic) that have been exploited widely in targeted approaches. To do so, high-resolution mass spectrometry was applied to thoroughly investigate selectivity, repeatabilit

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