Relevant bibliographies by topics / Hygienic Laboratory (U.S.)

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Hygienic Laboratory (U.S.)'

Author: Grafiati
Published: 4 June 2021
Last updated: 3 February 2022

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Journal articles on the topic "Hygienic Laboratory (U.S.)"

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Sienes Bailo, Paula, María Santamaría González, Silvia Izquierdo Álvarez, Raquel Lahoz Alonso, Patricia Serrano Frago, and José Luis Bancalero Flores. "A study of crystalluria: effectiveness of including hygienic-dietary recommendations in laboratory reports." Advances in Laboratory Medicine / Avances en Medicina de Laboratorio 2, no. 1 (February 10, 2021): 109–14. http://dx.doi.org/10.1515/almed-2020-0124.

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Abstract Objectives To assess the effectiveness of incorporating hygienic-dietary recommendations in laboratory reports in reducing the incidence of renal colic (RC). A study was performed to compare the incidence of RC in two groups of patients who had suffered at least a crystalluria event associated with the risk of urolithiasis. Recommendations were only incorporated in the laboratory reports of one group. Methods A retrospective observational study. The study sample was composed of patients who had at least an episode of crystalluria associated with a higher risk of urolithiasis. The laboratory reports of patients in Group A (n=1,115), treated in 2017, did not include any hygienic-dietary recommendations, whereas patients in Group B (n=1,692), treated in 2018, received hygienic-dietary recommendations through their laboratory reports. χ2 and Mann-Whitney U test were used to assess differences based on sex, age, and type of urinary crystals. Results The incidence of RC was 2.02 times higher in group A (2.24%) than in group B (1.12%). No significant differences were observed in the incidence of RC based on the type of urinary crystal. The incidence of RC was substantially higher in patients who suffered at least an event of crystalluria associated with a higher risk for urolithiasis as compared to the general population during the same period (0.46%, consistently with the incidence rates reported in the literature). Conclusions The incorporation of messages alerting on the risk of urolithiasis and the inclusion of hygienic-dietary recommendations in laboratory reports may be useful for reducing the incidence of RC.
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Adamović, Dragan. "Occupational safety in the medical laboratory and formaldehyde exposture." Sigurnost 62, no. 2 (July 8, 2020): 105–14. http://dx.doi.org/10.31306/s.62.2.7.

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SAŽETAK: Opasne kemijske tvari u raznim oblicima narušavaju kakvoću zraka u radnoj okolini. Način unošenja otrovnih tvari u ljudsko tijelo i transformacijski putovi glavni su čimbenici štetnih učinaka. Zbog velike raširenosti formaldehida u mnogim industrijama već nekoliko desetljeća pozornost stručnjaka zaštite na radu usmjerena je na taj fenomen. Studija prikazuje temeljne karakteristike formaldehida i učinke na zdravlje prouzročene izlaganjem različitim koncentracijama te opasne tvari. Težište je na zaposlenima u medicinskim laboratorijima gdje se formaldehid koristi kao sredstvo za dezinfekciju i agens za čuvanje uzoraka tkiva u anatomskim laboratorijima. Eksperimentalni dio studije odrađen je u laboratorijima Odjela za anatomiju, a razine koncentracije formaldehida praćene su u različitim prostorijama kako bi se utvrdila izloženost zaposlenika.
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Cleary, F. J. "THE MATERIAL LABORATORY, U. S. NAVY YARD, NEW YORK." Journal of the American Society for Naval Engineers 43, no. 1 (March 18, 2009): 54–104. http://dx.doi.org/10.1111/j.1559-3584.1931.tb03736.x.

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Purves, Byron. "QUALIFICATION STRATEGIES FOR THE INTERNATIONAL SPACE STATION U. S. LABORATORY." INCOSE International Symposium 6, no. 1 (July 1996): 1124–28. http://dx.doi.org/10.1002/j.2334-5837.1996.tb02131.x.

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Bukuya, Melinda, Teresa Martinelli, Joanne Tzouroutis, Elizabeth Duncan, Katie Welldon, Margaret Collecutt, Heather Aumann, Jim Thom, Grace Gilmore, and Emmanuel Favaloro. "A Comparative Multi-laboratory Assessment of Three Factor VIII/von Willebrand Factor Concentrates." Thrombosis and Haemostasis 87, no. 03 (2002): 466–76. http://dx.doi.org/10.1055/s-0037-1613027.

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SummaryFive expert laboratories have participated in a cross-laboratory study to co-evaluate and compare three commercial Factor VIII/von Willebrand factor (VWF) concentrates. A total of nine factor concentrate lots were evaluated, comprising AHF (High Purity) (AHF HP; X3), Biostate® (X3) and Humate/Haemate (X3). All laboratories blind tested for FVIII:C, VWF:Ag and VWF:CB, four tested for VWF:RCo, and one performed VWF:Multimers. The study yielded inter-laboratory CVs for VWF:Ag and FVIII:C around 10–15%, and for VWF:CB and VWF:RCo around 20%, significantly lower than those of previous multi-laboratory surveys. All three lots of AHF HP contained in the vicinity of 25 U/ml FVIII:C, around 60–75 U/ml of VWF:Ag, but only 30–45 U/ml of VWF:CB and 40–50 U/ml of VWF:RCo (thus, CB/Ag ratio around 0.5–0.6 and RCo/Ag ratio around 0.6–0.7). Study determined that FVIII:C and VWF:RCo levels were similar to manufacturer assigned levels. Some loss of the high molecular weight (HMW) multimers was observed, together with an intense low molecular weight (LMW) VWF band consistent with some reduction or proteolysis of HMW VWF. All three lots of Humate/Haemate contained in the vicinity of 23–32 U/ml of FVIII:C, 70–105 U/ml of VWF:Ag, 50–90 U/ml of VWF:CB and VWF:RCo (i. e. CB/Ag ratio around 0.6–0.9 and RCo/Ag ratio around 0.6–1.1). Study-determined FVIII:C and VWF:RCo levels were similar to manufacturer-assigned levels. The LMW multimer band seen with AHF HP was also observed with Humate/Haemate. All three lots of Biostate contained in the vicinity of 40–55 U/ml of FVIII:C, 105–170 U/ml of VWF:Ag, 90–150 U/ml of VWF:CB, and 90–135 U/ml of VWF:RCo (i. e. CB/Ag and RCo/Ag ratios around 0.7–1.0). Study-determined FVIII:C levels were similar to manufacturer-assigned levels. The LMW multimer band seen with AHF HP was not observed with Biostate. The defined pattern of increasing CB/ Ag from AHF HP to Humate/Haemate and Biostate was consistently observed in study data from each of the five laboratories. In conclusion, study findings indicate some differences in the retention of functional/ HMW VWF between factor concentrates, and this is expected to have significant implications in terms of clinical efficacy for therapy in VWD.
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Elder, S. A., and M. S. Korman. "Laboratory experiments in the acoustics course at the U. S. Naval Academy." Journal of the Acoustical Society of America 94, no. 3 (September 1993): 1854. http://dx.doi.org/10.1121/1.407679.

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LI, Liping, Junying TANG, Liang ZHAO, Songxin MA, Ying CHEN, Ying WANG, Yuhe YANG, and Xia ZUO. "Application of U-G-S Model in Collaborative Innovation for Chemistry Laboratory Education." University Chemistry 33, no. 4 (2018): 1–6. http://dx.doi.org/10.3866/pku.dxhx201709025.

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Stark, N., A. E. Hay, R. Cheel, L. Zedel, and D. Barclay. "Laboratory Measurements of Coarse Sediment Bedload Transport Velocity Using a Prototype Wideband Coherent Doppler Profiler (MFDop)." Journal of Atmospheric and Oceanic Technology 31, no. 4 (April 1, 2014): 999–1011. http://dx.doi.org/10.1175/jtech-d-13-00095.1.

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Abstract A prototype wideband coherent Doppler profiler (MFDop) was tested for measuring bedload velocity of different gravel and coarse-sand-sized fractions (d = 1–32 mm) in the laboratory. The sediment was spread out on a smooth-surface tray, and motion was initiated by tilting the tray at angles of α = 20°–39° from the horizontal. Particle velocities downslope (u), cross slope (υ), and vertical to the tray (w) were determined for different MFDop parameter settings, such as monostatic/bistatic configuration, acoustic beam angle, and pulse length. Video observations of bed particle velocity were made for comparison to the acoustic measurements. Velocities estimated using the MFDop equal to, on average, 71%–74% of the velocities determined using the video observations. Standard deviations ranged from 21% to 35%, including observed irregular motion. Three stages of sediment motion were observed: (i) single particles moving with u ≤ 5 cm s−1, (ii) varying motion of particles and particle groups with predominantly 5 cm s−1 ≤ u ≤ 20 cm s−1, and (iii) fast sheetlike motion with u ≥ 20 cm s−1. The cross-slope velocity υ and the vertical velocity w were significantly smaller than u, hinting at slipping as the major particle motion rather than rolling or saltation. Comparisons between MFDop and video-determined velocities showed good agreement. Standard deviations for the MFDop velocity estimates ranged from 22% to 35%. The trials with different gravelly sediments and coarse sand revealed a significant influence of grain size, as well as grain shape impacting the initiation of sediment transport and transport velocities.
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Baldovin, Tatjana, Irene Amoruso, Fabrizio Caldara, Alessandra Buja, Vincenzo Baldo, Silvia Cocchio, and Chiara Bertoncello. "Microbiological Hygiene Quality of Thermal Muds: A Pilot Study in Pelotherapy Facilities of the Euganean Thermal District (NE Italy)." International Journal of Environmental Research and Public Health 17, no. 14 (July 13, 2020): 5040. http://dx.doi.org/10.3390/ijerph17145040.

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Evaluation of hygienic aspects of thermal mud microbiology is still neglected. This study evaluates the microbiological hygiene quality of thermal muds, providing a comprehensive assessment of the whole mud cultivation chain. Maturing mud, peloid and used mud samples were collected twice in a year from 30 SPAs of the Euganean Thermal District, NE Italy. Samples were processed with an ad hoc laboratory method. The following indicator parameters were assessed: Total Count at 22, 37 and 55 °C; total coliforms; Escherichia coli; enterococci; Staphylococcus aureus; Pseudomonas aeruginosa; sulfite-reducing clostridia; dermatophytes. Statistical significance of differences between the two sampling campaigns and correlation between temperature and indicator parameters were evaluated. One-hundred eighty samples were analyzed. Widespread presence of environmental species was found, as well as hints of possible microorganism transfer from the patient’s skin to the mud. Proper setting of thermal water temperature resulted critical, in terms of hygienic quality. Although optimal maturation should be granted (thermal water at 30–42 °C), a pasteurization step at 60–65 °C is strongly recommended to sanitize peloids before pelotherapy. Facilities re-using thermal muds should also implement a regeneration step at ≥65 °C. Core evaluation of thermal mud hygienic quality could encompass the following guidelines: absence (i.e., 0 colony forming units (CFU)/g) of E. coli, P. aeruginosa, S. aureus and dermatophytes.
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YA. BERDNIKOV, A., YA A. BERDNIKOV, M. FABER, A. N. IVANOV, V. A. IVANOVA, A. V. NIKITCHENKO, and N. I. TROITSKAYA. "ON NONLEPTONIC DECAYS OF THE Λ0-HYPERON IN THE EFFECTIVE QUARK MODEL WITH CHIRAL U(3)×U(3) SYMMETRY." International Journal of Modern Physics A 22, no. 10 (April 20, 2007): 1835–47. http://dx.doi.org/10.1142/s0217751x07036373.

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Within the effective quark model with chiral U (3) × U (3) symmetry we calculate the S-wave and P-wave amplitudes and the partial widths of the nonleptonic decays of the Λ0-hyperon, Λ0 → pπ- and Λ0 → nπ0. The theoretical results agree well with the experimental data. The angular distributions of the decay rates in dependence on the polarizations of baryons are analyzed both in the laboratory frame and in the rest frame of the Λ0-hyperon.
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Dissertations / Theses on the topic "Hygienic Laboratory (U.S.)"

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Bahenská, Lucie. "Sledování parametrů funkce štítné žlázy s ohledem na diagnostiku hypothyreozy u osob středního věku." Master's thesis, 2013. http://www.nusl.cz/ntk/nusl-321917.

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Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Lucie Bahenská Supervisor: doc. MUDr. Bohuslav Matouš, CSc. Title of diploma thesis: Monitor the parameters of thyroid function with regard to a diagnosis of hypothyroidism in middle aged There was proved an occurence of the thyroid disorders with regard to hypothyreosis in humans in the middle age in the file of 15 412 patients (5753 men, 9659 women). Simultaneously there was calculated an occurence of these disorders in the rate on men vs women. There was a file of all patients examined in VFN during the year 2012 including all patients treating at the department of endocrinology. No significant higher occurence of thyroid disorders at women was found. In the examined file there were 474 TSH analysis indicated by a practitioners like a steering control. There wasn't confirmed a higher incidence of an occurence of the thyroid disorders at women. In the file indicated by practitioners there was changed the value of TSH at 7,2% women and 9,9% men. The reason can be caused by examination the men in the risk group in comparison to women, who are examined without risk factors. There was checked the connection between hypothyreosis and diabetes mellitus and no significant higher occurence...
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KOLÁŘOVÁ, Lucie. "Hygienické a pracovní návyky u levorukých žáků na 1. stupni ZŠ." Master's thesis, 2018. http://www.nusl.cz/ntk/nusl-375707.

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The diploma thesis deals with the hygienic and working habits of left-handed pupils at the first level of elementary schools. The thesis is divided into two main parts, theoretical and research. The theoretical part of the thesis deals with basic information about laterality, brain hemispheres and school maturity. The main focus is on leftist and writing, where we focus on preparation for writing and individual hygiene and working habits of pupils. Problems of written speech and famous leftists are also mentioned. The research part is focused on the survey of the level of hygienic and working habits of primary school pupils. The research was conducted in the form of observations. The obtained results are graphically processed and interpreted. In conclusion, there is a recommendation for working with left-handers in practice.
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Books on the topic "Hygienic Laboratory (U.S.)"

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Maljković, Teodora. Health effects of ash from coal gasification and interaction with heavy metals in rats =: Zdravstveni učinak šljake iz uplinjavanja ugljena i interakcija s teškim metalima u štakora. Zagreb: Jugoslavenska akademija znanosti i umjetnosti, 1988.

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Feasibility of Standardizing Automated Laboratory Analyzers On-Board U. S. Naval Ships. Storming Media, 1999.

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National Institute of Standards and Technology (U.S.), ed. ELECTRONICS AND ELECTRICAL ENGINEERING LABORATORY ELECTRICITY DIVISION, PROGRAMS, ACTIVITIES, AND ACCOMODATIONS... NISTIR 6842... U. S. DEPAR. [S.l: s.n., 2002.

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(Editor), Marilyn Johnston, Patti Brosnan (Editor), Don Cramer (Editor), and Tim Dove (Editor), eds. Collaborative Reform and Other Improbable Dreams: The Challenges of Professional Development Schools (S U N Y Series in Teacher Preparation and Development). State University of New York Press, 2000.

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Peake, James B., and Sephen J. Morrison. Defense Department's Enduring Contributions to Global Health: The Future of the U. S. Army and Navy Overseas Medical Research Laboratory. Rowman & Littlefield Publishers, Incorporated, 2011.

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Akasofu, Syun-Ichi. Dynamics of the Magnetosphere: Proceedings of the A. G. U. Chapman Conference 'Magnetospheric Substorms and Related Plasma Processes' Held at Los Alamos Scientific Laboratory, Los Alamos, N. M. , U. S. A. October 9-13 1978. Springer, 2012.

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Department of Defense. 21st Century Complete Guide to U. S. Navy Research: Naval Research Laboratory (NRL), Office of Naval Research (ONR), Navy Warfare Development Command ¿ History and Ongoing Projects, Materials, Radar, Rockets, Oceanography, Astronomy, Space Systems, Nanoscience, Fleet and Ship Innovations and Improvements, Sea Shield and Sea Strike Programs, Sensors, Bioengineering, Neural Computation. Progressive Management, 2003.

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Book chapters on the topic "Hygienic Laboratory (U.S.)"

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Li, Jie Jack, Chris Limberakis, and Derek A. Pflum. "Reductions." In Modern Organic Synthesis in the Laboratory. Oxford University Press, 2008. http://dx.doi.org/10.1093/oso/9780195187984.003.0010.

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The Barton deoxygenation (or Barton–McCombie deoxygenation) is a two-step reaction sequence for the reduction of an alcohol to an alkane. The alcohol is first converted to a methyl xanthate or thioimidazoyl carbamate. Then, the xanthate or thioimidazoyl carbamate is reduced with a tin hydride reagent under radical conditions to afford the alkane. Trialkylsilanes have also been used as the hydride source. Reviews: (a) McCombie, S. W. In Comprehensive Organic Synthesis; Trost, B. M.; Fleming, I., Eds.; Pergamon Press: Oxford, U. K., 1991; Vol. 8, Chapter 4.2: Reduction of Saturated Alcohols and Amines to Alkanes, pp. 818–824. (b) Crich, D.; Quintero, L. Chem. Rev. 1989, 89, 1413–1432. To a solution of the â-hydroxy-N-methyl-O-methylamide (0.272 g, 1.55 mol) in tetrahydrofuran (THF) (30 mL) were added carbon disulfide (6.75 mL, 112 mmol) and iodomethane (6.70 mL, 108 mmol) at 0 °C. The mixture was stirred at this temperature for 0.25 h, and then sodium hydride (60% suspension in mineral, 136.3 mg, 3.4 mmol) was added. After 20 min at 0 °C, the reaction was quenched by slow addition to 60 g of crushed ice. (Caution: hydrogen gas evolution!). The mixture was raised to room temperature and separated, and the aqueous layer was extracted with CH2Cl2 (4 × 15 mL). The combined organic extracts were dried (Na2SO4</aub>), concentrated in vacuo, and purified (SiO2, 5% EtOAc in hexanes) to afford 0.354 g (86%) of the xanthate. To a solution of the xanthate (2.95 g, 11.1 mmol) in toluene (100 mL) was added tributyltin hydride (15.2 mL, 56.6 mmol) and 2,2´-azobisisobutyronitrile (AIBN, 0.109 g, 0.664 mmol). The reaction mixture was then heated to reflux for 1 h. The mixture was cooled, concentrated in vacuo, and purified (SiO2, 100% hexanes to remove tin byproducts, followed by 10% EtOAc in hexanes to elute product) to afford 1.69 g (96%) of the N-methyl-O-methylamide.
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KOUTTAB, NICOLA M., SHASHIKANT R. MEHTA, and ABBY L. MAIZEL. "Human Lymphocyte Proliferation: Requirements for Activation and Growth**For the work done in this laboratory we acknowledge U. S. National Institutes of Health grant CA21927, and the grant from the Kleberg Foundation." In Biological Response Modifiers, 361–84. Elsevier, 1985. http://dx.doi.org/10.1016/b978-0-12-695770-9.50020-9.

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Swain, D. W., R. H. Goulding, F. W. Baity, R. I. Pinsker, J. S. deGrassie, C. C. Petty, and D. J. Hoffman. "Improved Tuning and Matching of Ion Cyclotron Systems**This research was sponsored by the Office of Fusion Energy, U. S. Department of Energy, under contract DE-AC05–96OR22464 with Oak Ridge National Laboratory managed by Lockheed Martin Energy Research Corp. and under contract DE-AC02-76-CHO3073 with General Atomics." In Fusion Technology 1996, 625–28. Elsevier, 1997. http://dx.doi.org/10.1016/b978-0-444-82762-3.50126-9.

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Swain, D. W., S. E. Attenberger, P. T. Bonoli, W. A. Houlberg, and W. M. Nevins. "Operating points and feedback control of plasma characteristics in tokamaks with full current drive**This research was sponsored by the Office of Fusion Energy, U. S. Department of Energy, under contract DE-AC05-84OR21400 with Martin Marietta Energy Systems, Inc., and under Contract DE-AC02-76-CHO3073 with Princeton Plasma Physics Laboratory." In Fusion Technology 1994, 719–22. Elsevier, 1995. http://dx.doi.org/10.1016/b978-0-444-82220-8.50147-3.

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Graf, William L. "Annual Plutonium Budget for the Rio Grande." In Plutonium and the Rio Grande. Oxford University Press, 1995. http://dx.doi.org/10.1093/oso/9780195089332.003.0013.

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A mean annual plutonium budget for the Northern Rio Grande provides an accounting of the amounts of plutonium moving into and out of various reaches of the river during a typical year. Such a budget is a basis for assessing the rates of plutonium transport and the location of storage along the river. The budget presented in the following pages is for bedload and suspended sediments. It does not include plutonium in water because water-borne plutonium is such a small portion of the total in the system (as discussed in Chapter 7). The budget as calculated here requires data concerning sediment and plutonium concentrations in the sediment. The sediment discharge data that are available from U. S. Geological Survey gaging sites (Chapter 4) define the overall framework for budget construction. A reasonably detailed picture is possible for the river system from the Rio Grande at Embudo and the Rio Chama at Chamita southward to the Rio Grande at San Marcial (for locations, see Figure 3.9) where the river empties into Elephant Butte Reservoir. Data collected by Los Alamos National Laboratory and published in the annual surveillance reports by the laboratory’s Environmental Studies Group and later by the Environmental Surveillance Group provide plutonium concentrations for bedload and suspended sediments. The calculations for each site in this study used mean values of plutonium concentrations from all measurements at or near the site. Table 8.1 reviews the sources of plutonium concentration data for each of the sediment-gaging sites in the regional budget calculations. Unfortunately, the sites for collecting the plutonium data were not always colocated with the gaging sites that produced the sediment discharge data. In addition, most of the plutonium concentration data are for bedload sediments because of the manner in which the workers collected samples. In some cases, the best estimates of plutonium concentrations in suspended load for gaging sites are from concentrations found in sediments of the nearest reservoir downstream because those sediments are likely to have been in suspension before their emplacement on reservoir floors. The assumption that the mean concentration is a useful representative value seems reasonable given that in those reaches with relatively large amounts of data, concentration values do not show temporal or geographic trends.
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Blythe, Jim, and Manuela Veloso. "An Analysis of Search Techniques for a Totally-Ordered Nonlinear Planner**This research was sponsored by the Avionics Laboratory, Wright Research and Development Center, Aeronautical Systems Division (AFSC), U. S. Air Force, Wright-Patterson AFB, OH 45433-6543 under Contract F33615-90-C-1465, Arpa Order No. 7597. The views and conclusions contained in this document are those of the authors and should not be interpreted as representing the official policies, either expressed or implied, of the U.S. Government." In Artificial Intelligence Planning Systems, 13–19. Elsevier, 1992. http://dx.doi.org/10.1016/b978-0-08-049944-4.50007-0.

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Conference papers on the topic "Hygienic Laboratory (U.S.)"

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DeGiorgi, Virginia G., Peter Finkel, Lauren Garten, and Margo Staruch. "Transduction Using Functional Materials: Basic Science and Understanding at the U. S. Naval Research Laboratory." In ASME 2019 Conference on Smart Materials, Adaptive Structures and Intelligent Systems. American Society of Mechanical Engineers, 2019. http://dx.doi.org/10.1115/smasis2019-5501.

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Abstract Recently NRL researchers have embarked on a basic research effort “Tuning Giant Magnetoelectric Properties in Phase Transformation Multiferroics” focused on multifunctional materials for energy transduction and conversion. Multiferroic materials combine at least two coupled ferroic properties and are used in multiple applications including magnetic field sensors, energy harvesting devices, non-volatile memory and antennas. There are very few single phase multiferroic materials, and they normally have relatively low magnetoelectric (ME) coupling coefficient. In contrast, engineered materials such as ME composites fabricated from piezoelectric and magnetostrictive materials can show multiple orders of magnitudes increase in the ME coupling coefficient. The optimal design of ME composites would lead to conditions of maximum response (strain, induced voltage, or field) with minimum applied electric or magnetic fields, providing advanced materials for transduction, sensing, energy harvesting and other applications. That is why NRL researchers are working on piezoelectric materials with enhanced properties due to a phase transformation that would minimize the stimuli needed to achieve large strains. Key to the successful design and fabrication of ME composites is an understanding of interface characteristics as well as individual material components. In this paper we will review the current status of work at NRL on engineered multiferroic composites comprised of piezoelectric and magnetostrictive materials coupled through strain. There are still many open questions about the interfacial properties as well as the individual component materials. Details will be presented from recent work on material characterization under repetitive cycling, interface characteristics, and stress/electric/thermal effects on driving the phase transition in a relaxor ferroelectric single crystal.
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Camacho, M., A. Fabra, F. Carretero, M. Borrell, I. Millet, and M. L. Rutllant. "A MONOCLONAL BASED ELISA FOR HUMAN u-PA QUANTIFICATION." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644844.

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An ELISA has been developped for quantifiing the anti gen levels of u-PA present in human plasma, tissue and cell extracts, conditioned medium and others biological fluids.The assay was set up using PVC plates coated with rabbit anti u-PA IgG and a monoclonal antibody against human u-PA as second antibody (UKM23 obtained in our laboratory as previously described).Detection was performed with a rabbit anti-mouse IgG conjugated with horseradish peroxidase.By immunoblotting technique the monoclonal antibody used UKM23 , recognizes all human molecular weight spe cies and an additional band of 81 KD in human plasma. Also recognizes the u-PA present in conditioned medium from HT-1080 cell line.The detection limit of ELISA assay is 0,1 ng of total u-PA.The first assay in human plasma from healty volun ters, shows u-PA levels of 4,39 ± 0,94 ng/ml.This study was supported by grants from the CAICYTn9 1258/81 and 3628/86.
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Nilsson, K., and P. Friberqer. "CHROMOGENIC ENDOTOXIN ASSAYS. - FURTHER METHOD DEVELOPMENT AND PRACTICAL EXPERIENCE." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644254.

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A kit for the determination of endotoxin by using Lumulus lysate (LAL) and a chromogenic substrate has now been available for three years. Here we will summarize the present status concerning its usefulness in the control of parenteral drugs, in hygienic control and in clinical diagnosis.The wide variation of uses, demands considerable work for method adaptation to the various types of specimen and laboratory. Almost all samples have to be pretreated to become compatible with the reagents in the assay. It has also been found to be important to consider variousdemands depending on the number of samples, how fast the answer is wanted, simplicity and accuracy of the assay that is needed as well as equipment available. In some cases a single screening test is required. Then a tube with 100 pi of LAL reagent can be taken from the freezer. The other extreme is the determination of hundreds of samples in each series. Then microplates and suitable readers can be of great help. Several other procedures used on special occasions allowing e.g. wide assay range or low reagent cost will also be presented.Manual and microplate procedures have been found suitable and can be made safe regarding contamination and losses of endotoxin. Single stage and micro methods have been tested and found to work. Data concerning material and laboratory equipment have been collected and are now available.By using a quantitative procedure forthe determination of the small amounts of endotoxin present in most samples, it has been possible to find optimal reaction conditions and to trace various kindsof errors. Procedures for method controland trouble shooting are also suggested.
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Triplett, D., K. Muagrave, T. Daniels, and E. J. W. Bowie. "IDENTIFICATION AND FURTHER CHARACTERIZATION OF TYPE IIE VON WILLEBRAND DISEASE." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644107.

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Type IIE von Willebrand disease has previously been identified in a single family with two affected members. We have identified a second family with five affected individuals. The pattern of inheritance is autosomal dominant and the clinical picture is characterized by mild mucocutaneous bleeding. Laboratory results for affected patients: VIII:C ranged from .62 u/ml to 1.0 u/ml, VWD antigen 2.0 u/ml to 4.75 u/ml, and ristocetin cofactor activity 0.04 u/ml to .32 u/ml. Bleeding times, PT and APTT results were within normal ranges.Multimeric analysis of both citrated plasma and platelet lysates utilizing sodium dodecyl sulfate-polyacrylamide electrophoresis was characterized by a decrease of the largest oligomers and the individual multimers were abnormal with a single identifiable band. Plasma samples collected in the presence of a variety of inhibitors had no effect on the multi-meric pattern. Intravenous infusion of DDAVP (.3 ug/kg of body weight) resulted in a slight increase in the highest molecular weight oligomers but no change in the ristocetin cofactor activity or individual multimeric patterns.
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Aramayo, Gustavo A. "Seismic Evaluation of the Oak Ridge National Laboratory High Flux Isotope Reactor Support Assembly." In ASME 2006 Pressure Vessels and Piping/ICPVT-11 Conference. ASMEDC, 2006. http://dx.doi.org/10.1115/pvp2006-icpvt-11-93378.

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The support assembly of the Oak Ridge National Laboratory High Flux Isotope Reactor (HFIR) was modeled to determine the assembly’s response to a seismic excitation. The compliance of this structural component to established U. S. Department of Energy (USDOE) standards [1, 2] is evaluated.
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Kalyan, N. K., S. G. Lee, W.-T. Hum, R. Hartzell, M. Levner, and P. P. Hung. "IN VITRO STUDIES ON THE BINDING OF TISSUE-TYPE PLASMINOGEN ACTIVATOR (t-PA) AND UROKINASE (u-PA) TO LIVER MEMBRANES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643603.

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The plasminogen activators, t-PA and u-PA, are glycoproteins known to be involved in homeostasis of the blood clotting system, and thus are of potential clinical use in the treatment of thrombosis. Several in vivo studies have shown that both t-PA and u-PA are quickly removed from the blood circulation, predominantly by the liver. The mechanism by which the liver removes these proteins is not understood. To delineate this, we conducted in vitro studies of binding of PAs or their derivatives to isolated mouse liver membranes utilizing a functional assay developed in our laboratory. The assay consisted of initial binding of t-PA to liver membranes followed by centrifugation to pellet the membranes and the assay of the activity of the membrane-bound t-PA by a fibrin-agar plate method. The bound t-PA, which retained complete enzymic activity, could be dissociated by SDS treatment in an undegraded form as shown by SDS-PAGE. The binding of t-PA as well as u-PA was very fast and did not compete with glycoproteins or sugars containing the terminal galactose, mannose and N-acetylglucosamine residues. Furthermore, the treatment of t-PA with neuraminidase and/or periodate oxidation did not affect its binding characteristics. These data suggest that the carbohydrate moieties of t-PA and u-PA, unlike many glycoproteins, do not mediate their binding to the liver. This raised the possibility of the liver binding sequence being located in the protein backbone, especially the non-protease domains which are known to determine the biological specificities of PAs. The relative binding of u-PA and its low molecular weight (LMW) derivative containing only the protease domain, to the liver membranes was studied. Unlike u-PA and t-PA, LMW-urokinase did not bind significantly. This suggests that the protein sequence containing the non-protease domains, rather than the carbohydrate moieties of PAs contain the information necessary for binding to the liver and possibly their clearance from the blood circulation.
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Hiles, Kirk E., and Gregg W. Dixon. "Enhancing Undergraduate Education: Design of a Gas Turbine Laboratory Facility." In ASME 1996 International Gas Turbine and Aeroengine Congress and Exhibition. American Society of Mechanical Engineers, 1996. http://dx.doi.org/10.1115/96-gt-190.

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An undergraduate gas turbine laboratory facility was designed and installed by four senior Mechanical Engineering students for their capstone design project at the U. S. Coast Guard Academy. The seniors instrumented a 65 horsepower gas turbine auxiliary power unit from an HH-3F Pelican helicopter and installed it in the existing engine laboratory. The objective of this project was to provide an opportunity for engineering students to better understand thermodynamic principles of gas turbine operation through hands-on experimentation. The laboratory facility was designed to allow students to determine the performance characteristics of the T-62-16B gas turbine and relate them to a Brayton cycle model. This paper details the installation and instrumentation of the gas turbine, the design of the data acquisition system, the results obtained with initial system tests, and future experimental plans.
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Lorenz, R., M. Holweger, GE Vogel, and M. Classen. "SUBSTITUTION THERAPY WITH ANTITHROMBIN III: CLINICAL EXPERIENCE WITH DECOMPENSATED LIVER CIRRHOSIS AND HEMORRHAGIC COMPLICATIONS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643069.

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In patients with decompensated liver cirrhosis, the coagulation disorder requires therapy only when bleeding complications occur. As therapy involves the risk of triggering a consumption coagulopathy, the discussion here examines the beneficial effect of boosting the inhibitor Potential by supplementing the administration of fresh plasma with substitution of AT III concentrate. To investigate this question, we conducted a retrospective study on the clinical course in 19 patients with decompensated liver cirrhosis, hepatic encephalopathy and bleeding complications.The following treatment scheme was employed uniformly in all 19 cases: substitution of vitamin K 20 mg/d, heparin 100 - 300 U/h and fresh plaspna 2-4 bags/d. AT III concentrate (3 - 8 × 500 U/d Ky-bemin HS ) was administered additionally in group 1 (n=8), but was omitted in group 2 (n= 11). Both groups were statistically comparable with regard to initial laboratory findings (e.g. bilirubin, group 1: 11.7 ± 3.6 mg/dl; bilirubin, group 2: 10 ± 2.9 mg/dl) and encephalopathy Tcoma stages 1-2), with the exception of the two survivors in group - who had been in a precomatose stage (stages 0-1) and had bilirubin levels of 3 ± 1.5 mg/dl.A comparison oT both collectives shows a lethality of 62.5 % (n=5) for group 1, and of 81 % (n=9) for group 2. Clinically, the incidence of hemorrhagic complications in group 1 was 61 %, but 100 % in group 2, although laboratory analysis showed no differences in the parameters of systemic coagulation. As regards the other laboratory values, including the transaminases and blood count, there were only differences in the development of the bilirubin values which, in group 1, rose to -3 ± 4 mg/dl only during the terminal phase, but which, in group 2, began to rise continuously from the second day of treatment onwards 2nd reached values of 28 ± 8.6 mg/dl.It follows that, in patients with decompensated liver cirrhosis and bleeding complications, the administration of AT III concentrate (Ky-bernin HS ) as a supplement to conventional coagulation therapy, increases the survival rate and reduces the incidence of further bleeding. It is, however, not possible tc explain the cause of this effect from the analysis of the laboratory data for the systemic coagulation. Therefore, one may hypothesize that the effect is due to beneficial effect of AT III within microcirculation.
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Lillicrap, D., S. Windsor, Benford H. Hoogendorn, and A. R. Giles. "PLATELET VON WILLEBRAND FACTOR: STUDIES IN TYPE II VON WILLEBRAND′S DISEASE VARIANTS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644108.

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Recent evidence suggests that platelet von Willebrand factor (vWf) plays an important role in maintenance of primary haemostasis. We have studied a normal population (N=24) and 15 patients with variant forms of von Willebrand's disease (vWd) to determine the utility of platelet vWf:Ag measurement in this disorder. Citrated blood was spun at 100 g for 10 mins at 20°C to prepare platelet rich plasma (PRP). The PRP was removed and platelet count and mean platelet volumes (MPV) were determined on a Coulter S+ counter. A platelet button was prepared by centrifugation of PRP at 3,500 g for 15 mins. Plasma supernatant was aspirated and replaced with 900 nl of DH^O. Platelets were lysed by 5 cycles of freeze/thawing and membrane debris pelleted by centrifugation at 13,000 g for 10 mins. Platelet lysate vWf:Ag was measured by a polyclonal anti-vWf ELISA. All platelet samples were tested at least twice. Bleeding times (BT), platelet sensitivity to ristocetin and other factor VIH/vWf parameters were measured by standard methods. vWf molecular weight profiles were assessed by crossed Immunoelectrophoresis and/or by multimer analysis. In 24 normal individuals, mean vWf:Ag was 31 u/109 platelets (7-68). The mean MPV for this group was 8.0 fl. No correlation was seen between MPV and vWf:Ag content. 15 vWd variant patients were studied. No platelet vWf:Ag was found in one type III patient. In two Type Ila patients mean platelet vWf:Ag was 57 u/109 pits. Patients with laboratory features of Type lib vWd showed two patterns of platelet vWf:Ag content. In two families (5 patients) the mean platelet vWf:Ag was only 24 u/109 pits. In this group the MPV was 9.42 fl. and the mean BT 8.75 mins. In the remaining seven Type IIb patients (4 families), the mean platelet vWf:Ag was 105 u/109 pits., MPV 9.1 fl. and mean BT 8 mins. These results in Type lib vWd suggest further heterogeneity within this disease subtype. The finding of a markedly elevated platelet vWf:Ag appears to identify one group of Type lib vWd patients.
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Drouin, J., D. Lillicrap, A. R. Giles, C. A. Izaguirre, S. Windsor, and H. Hoogendorn. "ABSENCE OF A BLEEDING TENDENCY IN SEVERE ACQUIRED DEFICIENCY OF PLASMA VON WILLEBRAND FACTOR (vWf)/F. VIII WITH NORMAL PLATELET vWf/F. VIII INDICES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644116.

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A 67 year old male with IgA myeloma has been investigated for a severe deficiency of plasma vWf/F. VIII but normal platelet vWf/F. VIII. He has no personal history nor family history of bleeding problems. He was initially investigated for a prolonged APTT of 43 secs. (25-40) obtained in a preoperative clotting screen. During this investigation he was found to have IgA myeloma. In retrospect, an APTT prior to uneventful coronary artery bypass surgery two years previously had been prolonged. Routine investigation has shown that platelet count and bleeding time have been repeatedly normal. Plasma F. VIII:C is 0.08 u/ml., F. VIII:Cag 0.07 u/ml., vWf:Ag 0.05 u/ml. and ristocetin cofactor 0.05 u/ml. In contrast, platelet values for vWf:Ag of 53 units/109 platelets and F. VIII:Cag of 176 units/109 platelets are within the normal ranges for our laboratory. The platelet lysate vWf multimer pattern is also normal. Patient's plasma shows inhibitory activity against vWf:Ag but not against either F. VIII:C or ristocetin cofactor activity. When patient plasma is incubated for 60 mins at 37°C with vWf and analysed by crossed Immunoelectrophoresis (CIE) for vWf:Ag, a double arc precipitin line is observed with marked retardation of the first arc. A similar vWf:Ag CIE double precipitin arc is seen following the infusion of cryoprecipitate. T 1/2 for F. VIII:C and vWf:Ag are both reduced following the infusion of cryoprecipitate - F. VIII:C 2 hrs, vWf:Ag 3 trs. No secondary rise in F. VIIIrC is seen at 24 hrs. Despite severe deficiency of plasma vWf/F. VIII, this man does not have a clinical bleeding tendency. We postulate that his plasma vWf/F. VIII deficiency is the result of complexing of his IgA myeloma protein with vWf, resulting in premature clearance of the vWf/F. VIII complex. This case further emphasizes the role of platelet associated coagulation factors in maintaining normal haemostasis.
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Reports on the topic "Hygienic Laboratory (U.S.)"

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Hoppel, Christopher, John H. Beatty, Jonathan S. Montgomery, James M. Bender, and Travis A. Bogetti. Metal Matrix Composites for Ordnance Applications. NDIA Firepower Symposium Held in U. S. Army Research Laboratory, Aberdeen Proving Ground, MD on 20 Jun 2001. Fort Belvoir, VA: Defense Technical Information Center, June 2001. http://dx.doi.org/10.21236/ada385804.

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Morgan, Susan, Alexandra Mosser, and John Bixby. University of Miami Laboratory for Integrative Knowledge (U-LINK) Program Evaluation Report. University of Miami, 2020. http://dx.doi.org/10.33596/ovprs-19-2.

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As one of the Roadmap Initiatives, U-LINK (University of Miami Laboratory for INtegrative Knowledge) is the University of Miami’s (UM’s) program to support innovative, problem-based interdisciplinary research. The U-LINK initiative is premised on the idea that the most significant challenges facing humanity, and therefore the most important research problems, require innovative and integrative approaches resulting from collaborations that bridge disciplines. The goal of U-LINK is to bring together faculty and trainees from multiple disciplines in collaboration with community stakeholders to develop and act on a shared vision of innovative solutions to grand societal challenges. To accomplish this goal, U-LINK provides training and funding opportunities for research teams, identifies common space(s) for teams to work together, creates interdisciplinary opportunities for UM undergraduate and graduate students, and helps UM faculty identify and pursue collaborative initiatives with faculty in other departments, schools, and colleges. This document details the features of the U-LINK program and provides data about outcomes of the program through 2019.
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An epidemiologic study of mortality and radiation-related risk of cancer among workers at the Idaho National Engineering and Environmental Laboratory, a U. S. Department of Energy facility. U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, January 2005. http://dx.doi.org/10.26616/nioshpub2005131.

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Methods of analysis by the U. S. Geological Survey National Water Quality Laboratory; determination of organic plus inorganic mercury in filtered and unfiltered natural water with cold vapor; atomic fluorescence spectrometry. US Geological Survey, 2001. http://dx.doi.org/10.3133/wri014132.

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