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Journal articles on the topic 'Hyperplexia'

Author: Grafiati

Published: 7 June 2025

Last updated: 2 August 2025

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1

Eroglu, Arzu, and Huseyin Caksen. "Giant axonal neuropathy: A rare disease hidden in polyneuropathy." Neurology Asia 29, no. 4 (2024): 1027–34. https://doi.org/10.54029/2024zme.

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Background& Objective: Giant axonal neuropathy (GAN) is a serious progressive neurodegenerative disease. The aim of this study is to evaluate the frequency and phenotypic-genotypic characteristics of GAN patients, which, like many rare diseases, is disguised under the name of polyneuropathy, and to present our experience. Methods: In this retrospective observational study, 105 pediatric patients with polyneuropathy were screened. Demographic characteristics and clinical diagnoses were reviewed. The mean age of the patients was 10.9 years (2-18), 59 were boys (56%) and 46 were girls (44%).

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Srinivas, G., D.V. Ramanjaneyulu, E. Muralinath, et al. "A Fundamental Parameters of Neonatal Seizures Include Diagnosis, Differential Diagnosis, Treatment, Prognosis and Complications." Journal of Advanced Research and Reviews in Medical & Medicine 2, no. 1 (2025): 34–40. https://doi.org/10.5281/zenodo.15341929.

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<em>One neurologic disorder that is often seen, particularly in newborns, is neonatal seizures. The abrupt, paroxysmal, aberrant change in electrographic activity from birth till the end of the neonatal period is how they are described. At this stage, the newborn brain is still developing immaturely. Because of this, newborn seizures have a distinct aetiology and electrographic findings, which might result in clinical presentations that are different (and more challenging to recognise) than those of later age groups. The assessment and management of newborn seizures are explained in this activ

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Panakkal, Samantha, Brian Falkenstein, Akif Burak Tosun, et al. "Abstract 454: TumorMapr™ analytical software platform: Unbiased spatial analytics and explainable AI (xAI) platform for generating data, extracting information, and creating knowledge from multi to hyperplexed fluorescence and/or mass spectrometry image datasets." Cancer Research 82, no. 12_Supplement (2022): 454. http://dx.doi.org/10.1158/1538-7445.am2022-454.

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Abstract Background: The current computational analyses of multi to hyperplexed fluorescence and/or mass spectrometry image datasets from patient pathology samples are not powerful enough to extract the maximum amount of information or to create the detailed knowledge that is required to advance precision medicine in pathology, including the development of personalized therapeutic strategies, identification of potential novel targets for drug discovery, selection of optimal patient cohorts for clinical trials, and improvement of the predictive power of prognostics/diagnostics. Methods: TumorMa

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Almeida, Pedro Machado, François Rivest, Quentin Juppet, et al. "Abstract 1716: Mapping the cellular architecture of the tumor microenvironment by integrating hyperplex immunofluorescence and automated image analysis." Cancer Research 82, no. 12_Supplement (2022): 1716. http://dx.doi.org/10.1158/1538-7445.am2022-1716.

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Abstract The tumor microenvironment (TME) is composed of malignant cells and the surrounding healthy counterpart. The precise identification of the TME components is crucial to understanding how this microecosystem remodels during tumorigenesis and responds to treatment in order to identify its vulnerabilities and treatment opportunities (1). In the past decade, significant efforts have been made to describe the TME using RNA-based technologies (2,3). These approaches shed light on the tumor heterogeneity and variable response to treatment. However, RNA-based biomarker expression profiling has

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Navikas, Vytautas, Quentin Juppet, Samuel Aubert, Benjamin Pelz, Joanna Kowal, and Diego Dupouy. "Abstract 4620: Automated multiplex immunofluorescence workflow to interrogate the cellular composition of the tumor microenvironment." Cancer Research 83, no. 7_Supplement (2023): 4620. http://dx.doi.org/10.1158/1538-7445.am2023-4620.

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Abstract Background: The tumor microenvironment (TME) is a constantly changing niche due to dynamic interactions between tumor cells and their surroundings. Recently, it became evident that a better understanding of the TME is needed to target the disease more accurately (Binnewies et al., Nat Med 2018; Vitale et al., Nat Med 2021). Spatial hyperplex immunofluorescence enables the visualization of cellular and non-cellular tissue components simultaneously. However, the increasing number of biomarkers detected by spatial proteomics quickly escalates the complexity of images and renders their in

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Au, Qingyan, Jun Fang, Anna Juncker-Jensen, et al. "Characterization of Myeloid-Derived Suppressor Cells and Tumor Associated Macrophages Using MultiOmyxTM Hyperplexed Immunofluorescence Assay in Hodgkin Lymphoma." Blood 132, Supplement 1 (2018): 4135. http://dx.doi.org/10.1182/blood-2018-99-115434.

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Abstract Tumor microenvironment (TME) consists of heterogeneous subsets of myeloid cells and plays a crucial role in promoting cancer development and metastasis. Tumor associated macrophages (TAMs) and myeloid-derived suppressor cells (MDSCs) all contribute to an immunologically permissive microenvironment for cancer cells. On basis of the expression of surface markers, MDSC can be further subdivided into granulocytic MDSC (G-MDSC, polymorphonuclear MDSC) and monocytic MDSC (M-MDSC). In solid tumors, these different myeloid cell populations are well characterized and extensively studied. Howev

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Yaseen, Hadeel, Hassanain Khudaier, and Ali Ibrahim. "Review of Histopathological Diagnoses of One Year Appendectomy Specimens in Sulaimani City." Iraqi National Journal of Nursing Specialties 26, no. 2 (2013): 102–15. http://dx.doi.org/10.58897/injns.v26i2.176.

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Objective: To review and see the pattern of histopathological diagnoses of one year appendectomy specimens.Methodology: This retrospective study was carried in Sulaimani Teaching Hospital over the period of one year (from 1stof January to 31st of December 2009). All pathological reports were reviewed retrospectively for patient’s age, sex,histopathological diagnosis and operative findings (if present). Histopathological diagnoses then were classified intoeither positive or negative for acute inflammation. Any associated findings or any surgical specimen removed with theappendix was recorded. T

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Pullara, Filippo, Brian Falkenstein, Bruce Campbell, et al. "Abstract 5445: Segmentation-free analysis of multiplexed images with unbiased spatial analytics and explainable AI for predicting disease outcomes." Cancer Research 83, no. 7_Supplement (2023): 5445. http://dx.doi.org/10.1158/1538-7445.am2023-5445.

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Abstract Background: The spatial intratumor heterogeneity (ITH) is widely acknowledged as driving therapeutic response and providing fuel for drug resistance. Currently, patient selection for immunotherapy is driven mostly by PD-1/PD-L1 based IHC tests and mutational analysis. These oversimplified approaches fail to predict the risk of recurrence, therapeutic response and drug resistance with high accuracy. We hypothesize that functional responses of heterogeneous non-random spatial arrangements of tumor, stromal and immune cells in the tumor microenvironment are determined by distinct combina

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Welle, Kevin A., Tian Zhang, Jennifer R. Hryhorenko, Shichen Shen, Jun Qu, and Sina Ghaemmaghami. "Time-resolved Analysis of Proteome Dynamics by Tandem Mass Tags and Stable Isotope Labeling in Cell Culture (TMT-SILAC) Hyperplexing." Molecular & Cellular Proteomics 15, no. 12 (2016): 3551–63. http://dx.doi.org/10.1074/mcp.m116.063230.

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Dephoure, N., and S. P. Gygi. "Hyperplexing: A Method for Higher-Order Multiplexed Quantitative Proteomics Provides a Map of the Dynamic Response to Rapamycin in Yeast." Science Signaling 5, no. 217 (2012): rs2. http://dx.doi.org/10.1126/scisignal.2002548.

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Kumar, Ajay, Shilpa Jamwal, Mukul Kumar Midha, et al. "Dataset generated using hyperplexing and click chemistry to monitor temporal dynamics of newly synthesized macrophage secretome post infection by mycobacterial strains." Data in Brief 9 (December 2016): 349–54. http://dx.doi.org/10.1016/j.dib.2016.08.055.

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Dekhnich, А. V., A. Yu Kuzmenkov, D. A. Popov, I. V. Shlyk, and M. V. Edelshtein. "Algorithm for the selection of drugs for targeted antimicrobial therapy based on the results of molecular biological studies of positive blood cultures." Messenger of ANESTHESIOLOGY AND RESUSCITATION 20, no. 2 (2023): 96–107. http://dx.doi.org/10.24884/2078-5658-2022-20-2-96-107.

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Cardinal changes in approaches to the choice of antimicrobial therapy for severe infections have occurred in recent years. They are associated with the growth of antibiotic resistance of nosocomial pathogens and the lack of sufficiently effective «universal» schemes of empirical antibiotic therapy. Recent international and domestic recommendations focus on a «pathogen-specific» approach aimed at the treatment of infections caused by specific problematic resistant pathogens. The application of such «pathogen-specific» recommendations is not possible without the availability of appropriate quali

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Rivest, François, Victor de Gautard, Vytautas Navikas, et al. "Abstract 4616: Automated multiplex immunofluorescence enables single cell analysis of tumor stroma." Cancer Research 83, no. 7_Supplement (2023): 4616. http://dx.doi.org/10.1158/1538-7445.am2023-4616.

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Abstract Background: The tumor microenvironment (TME) consists of malignant cells and supporting non-malignant cellular and non-cellular components that form the tumor stroma. The tumor stroma plays an important role in tumor progression and has emerged as a modulator of anti-tumor immunity (Salmon et al., Nat Rev Cancer 2019) and responses to therapy (Hirata and Sahai, Cold Spring Harb Perspect Med 2017). As such, several therapeutic approaches have recently been developed to target stromal cells as anti-cancer treatments (Valkenburg et al., Nat Rev Clin Oncol 2018, Bejarano et al., Cancer Di

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Au, Qingyan, Arezoo Hanifi, Erinn Parnell, et al. "Phenotypic Characterization of the Immune Landscape in the Bone Marrow of Patients with Acute Myeloid Leukemia (AML) Using MultiOmyxTM Hyperplexed Immunofluorescence Assay." Blood 134, Supplement_1 (2019): 1455. http://dx.doi.org/10.1182/blood-2019-122206.

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Background: Acute myeloid leukemia (AML) is a clinically and molecularly heterogeneous disorder. Bone marrow (BM) constitutes the home niche for leukemia cells in AML. Emerging data indicates that the BM microenvironment becomes immunosuppressive and plays a crucial role in cancer development and progression. Regulatory T cells (Treg), tumor associated macrophages (TAM) and myeloid-derived suppressor cells (MDSC) all contribute to immunologically permissive microenvironment for cancer cells. Based on phenotypical characteristics, MDSC can be further subdivided into granulocytic MDSC (G-MDSC, p

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Yin, Hong-Rui, Lei Zhang, Li-Qi Xie, et al. "Hyperplex-MRM: A Hybrid Multiple Reaction Monitoring Method Using mTRAQ/iTRAQ Labeling for Multiplex Absolute Quantification of Human Colorectal Cancer Biomarker." Journal of Proteome Research 12, no. 9 (2013): 3912–19. http://dx.doi.org/10.1021/pr4005025.

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Franco-Barraza, Janusz, Bhaumik Shah, Mariia Dmitrieva, et al. "Abstract 6468: Dissecting the immune landscape in EBV+ classical Hodgkin lymphoma following IL-23 inhibition using single-cell hyperplex immunofluorescence analysis: A case report." Cancer Research 85, no. 8_Supplement_1 (2025): 6468. https://doi.org/10.1158/1538-7445.am2025-6468.

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Abstract Background: The immune tumor microenvironment (TME) in Epstein-Barr Virus (EBV)+ classic Hodgkin lymphoma (cHL) displays a T helper (Th) 1 profile typical of effective antitumor response with increased infiltration of CD8+ T-cells. cHL cases showing evidence of interleukin (IL)-23 /IL-17 axis activation are usually EBV-. The TME of EBV+ cHL developed after IL-23 repressive therapy has not been reported. We report the TME characteristics of EBV+ cHL in a psoriasis patient on IL-23 inhibition therapy. Case Presentation: A 40-year-old male with chronic plaque psoriasis underwent Guselkum

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Au, Q., H. Nunns, E. Parnell, et al. "89P Spatial analysis using MultiOmyx hyperplex assay reveals high prevalence of mature tertiary lymphoid structure (TLS) and PDL1 expression in the MSI-high colorectal carcinoma (CRC)." Annals of Oncology 35 (December 2024): S1438. https://doi.org/10.1016/j.annonc.2024.10.111.

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van den Broek, Thijs, Raoull Hoogendijk, Mariëtte Kranendonk, et al. "TMIC-86. SPATIAL ORGANIZATION OF THE PEDIATRIC HIGH-GRADE GLIOMA TUMOR-IMMUNE LANDSCAPE." Neuro-Oncology 25, Supplement_5 (2023): v298. http://dx.doi.org/10.1093/neuonc/noad179.1151.

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Abstract BACKGROUND Advances in cellular immunotherapies have shown promising results for brain tumors, including pediatric-type diffuse high-grade gliomas (pHGG). A comprehensive understanding of the tumor-immune microenvironment (TME) is essential for efficient target identification and novel therapeutic strategies. The interplay between cells and their spatial localization within the TME may affect immune cell phenotype and function. We characterized these interactions and phenotypes by single-cell (SC) spatial proteomic analysis. METHODS Thirty-two tissue biopsies of pHGGs were collected f

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Jones, Jennifer C., Ashley Oliver, Jodi Hagen, Kristine Trueman, Grace Leland, and Alexander Kalyuzhny. "Abstract 2093: Evaluation of IDH1, EGFR, IGF1R and Ki67 biomarkers in glioblastoma using Lunaphore COMET platform." Cancer Research 85, no. 8_Supplement_1 (2025): 2093. https://doi.org/10.1158/1538-7445.am2025-2093.

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Abstract Glioblastoma (GBM) is the most aggressive primary neuroepithelial tumor diagnosed in the United States with approximately 14, 000 diagnoses each year. Patients with GBM have a poor prognosis and only 5% of these patients survive for more than 5 years. Diagnosis of GBM requires tumor biopsies with consideration of histopathological and molecular characteristics. Typically, IHC is done using a single-biomarker HRP-DAB detection technique which is not suitable for the simultaneous spatial analysis of multiple biomarkers. Specific markers involved with the histopathology of glioblastomas

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Parnell, Erinn A., Jiong Fei, Harry Nunns, et al. "Abstract 4688: Comprehensive analysis of natural killer cell-associated markers using MultiOmyxTM immunofluorescence assay." Cancer Research 83, no. 7_Supplement (2023): 4688. http://dx.doi.org/10.1158/1538-7445.am2023-4688.

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Abstract As a promising alternative platform for cellular immunotherapy, natural killer cells (NK) have recently gained attention as an important type of innate immune regulatory cell. NK cell immunotherapy approaches have been translated into clinical applications, and clinical trials of NK cell infusion in patients with hematological malignancies (HM) and solid tumors have thus far yielded many encouraging clinical results. Understanding the pattern of NK expression and the relationship to different states of NK cells may have direct relevance for immune responses in cancer. Approaches capab

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Lahdenranta, Johanna, Kristen Hurov, Heather Cohen, et al. "Abstract 5301: Tumor-targeted activation of CD137 using Bicycles: New insights into mechanism of action and discovery of BT7455, a clinical candidate for the treatment of EphA2-expressing cancers." Cancer Research 84, no. 6_Supplement (2024): 5301. http://dx.doi.org/10.1158/1538-7445.am2024-5301.

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Abstract Introduction: Clinical studies in cancer patients have validated CD137 agonism as an activator of the immune system to enable tumor rejection. We have demonstrated that small, chemically synthetic bicyclic peptides can drive tumor-localized agonism of CD137 and anti-tumor immunity in mouse models. Here, we report the next stage of our work - delving into the mechanism of action of these novel agents and extending our program to serve patients whose tumors express EphA2. Experimental Procedures: MultiOmyx™ hyperplexed immunofluorescence assay was used to evaluate the expression of CD13

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Tay, Norbert Sheng Cong, Yan Fen Peng, Heike Grabsch, and Anand D. Jeyasekharan. "Abstract 6526: Spatial characterisation of the tumour immune microenvironment in ARID1A-deficient gastric cancer." Cancer Research 84, no. 6_Supplement (2024): 6526. http://dx.doi.org/10.1158/1538-7445.am2024-6526.

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Abstract AT-Rich Interacting Domain 1A (ARID1A) is an important component in SWI/SNF Complex, a chromatin remodeller and an established tumor suppressor gene that is mutated in a variety of cancer types. This includes endometrial (~45%), bladder (~20%), and gastric (~15%). ARID1A mutations usually lead to a loss of protein expression or function, which results in tumor progression. The loss of ARID1A has been linked to genomic instability, potentially making the tumor more immunogenic/amenable to immune checkpoint inhibitor therapy. However, there is a discrepancy between clinical and mechanis

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Kaplan, Henry, Alexa Dowdell, Racheli Ben Shimoli, et al. "Abstract PO3-24-01: Mapping inter- and intra-tumor heterogeneity in Ductal Carcinoma in situ and invasive breast cancer using integrative multi-omic profiling." Cancer Research 84, no. 9_Supplement (2024): PO3–24–01—PO3–24–01. http://dx.doi.org/10.1158/1538-7445.sabcs23-po3-24-01.

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Abstract Background: Molecular profiling of ductal carcinoma in situ (DCIS) has shown some prognostic utility in the clinic. However, there is still an incomplete understanding of the diversity of molecular mechanisms by which DCIS progresses to invasive breast cancer (IBC).Here, we utilize integrative multi-omic profiling of co-occurring DCIS and IBC in humans as a model for mapping the relationship between tumor mutations, global gene expression and morphological changes in DCIS and IBC. Methods: We performed targeted panel DNA sequencing (Tempus xT), whole transcriptome profiling and hyperp

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Rosenbaum, Sara, Nathalie Fiaschi, Wei Keat Lim, et al. "Highly Multiplexed Immunohistochemistry Can Predict Steroid Refractory Gastrointestinal (GI) Acute Gvhd at the Time of Endoscopy." Blood 142, Supplement 1 (2023): 478. http://dx.doi.org/10.1182/blood-2023-189766.

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Background: For patients with high-risk hematologic disease, hematopoietic cell transplant (HCT) often represents the only option for cure. However, HCT is fraught with complications, leading to morbidity and mortality. Acute GVHD (aGVHD), specifically steroid refractory (SR) GI aGVHD, is a major cause of early mortality. Two issues with SR GI aGVHD predominate: (1) We do not understand the mechanisms differentiating SR vs steroid sensitive (SS) GI aGVHD; (2) We do not have an accurate test to predict SR vs SS GI aGVHD at the time of endoscopic evaluation. Here, we used state-of-the-art multip

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Andayani, Novi, Yeni Eliyanti, and Siska Ayu Ningsih. "The Effect of Benson Relaxation on Pain in Patients with Postoperative Benign Prostate Hyperplesia (BPH) at Sobirin Hospital, Kabupaten Musi Rawas." ANJANI Journal (Medical Science & Healthcare Studies) 1, no. 2 (2021). http://dx.doi.org/10.37638/anjani.v1i2.329.

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Nursing interventions or independent actions that can be done in reducing pain in patients with BPH postoperative one of which is by teaching Benson relakasasi techniques. The research problem is that there are still many postoperative BPH patients with pain. The purpose of this study was to determine the effect of Benson's relaxation on pain in patients with postoperative benign prostatic hyperplesia (BPH) at Sobirin Hospital Musi Rawas District. This type of research is a quasi experiment. The population was 94 people and the study sample was 10 people. The type of data used in research is t

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Bolognesi, Maddalena M., Lorenzo Dall’Olio, Amy Maerten, Simone Borghesi, Gastone Castellani, and Giorgio Cattoretti. "Seeing or believing in hyperplexed spatial proteomics via antibodies: New and old biases for an image-based technology." Biological Imaging 4 (2024). http://dx.doi.org/10.1017/s2633903x24000138.

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Abstract Hyperplexed in-situ targeted proteomics via antibody immunodetection (i.e., >15 markers) is changing how we classify cells and tissues. Differently from other high-dimensional single-cell assays (flow cytometry, single-cell RNA sequencing), the human eye is a necessary component in multiple procedural steps: image segmentation, signal thresholding, antibody validation, and iconographic rendering. Established methods complement the human image evaluation, but may carry undisclosed biases in such a new context, therefore we re-evaluate all the steps in hyperplexed proteomics. We foun

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Wu, Zhen, Weirong Xiang, Lin Huang, Shuwei Li, and Xumin Zhang. "Hyperplexing Approaches for up to 45-Plex Quantitative Proteomic Analysis." Analytical Chemistry, March 14, 2023. http://dx.doi.org/10.1021/acs.analchem.3c00237.

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Rivest, François, Deniz Eroglu, Benjamin Pelz, et al. "Fully automated sequential immunofluorescence (seqIF) for hyperplex spatial proteomics." Scientific Reports 13, no. 1 (2023). http://dx.doi.org/10.1038/s41598-023-43435-w.

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AbstractTissues are complex environments where different cell types are in constant interaction with each other and with non-cellular components. Preserving the spatial context during proteomics analyses of tissue samples has become an important objective for different applications, one of the most important being the investigation of the tumor microenvironment. Here, we describe a multiplexed protein biomarker detection method on the COMET instrument, coined sequential ImmunoFluorescence (seqIF). The fully automated method uses successive applications of antibody incubation and elution, and i

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Wu, Zhen, Yi Shen, and Xumin Zhang. "TAG-TMTpro, a Hyperplexing Quantitative Approach for High-Throughput Proteomic Studies." Analytical Chemistry, September 6, 2022. http://dx.doi.org/10.1021/acs.analchem.2c02099.

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Watson, Spencer S., Benoit Duc, Ziqi Kang, et al. "Microenvironmental reorganization in brain tumors following radiotherapy and recurrence revealed by hyperplexed immunofluorescence imaging." Nature Communications 15, no. 1 (2024). http://dx.doi.org/10.1038/s41467-024-47185-9.

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AbstractThe tumor microenvironment plays a crucial role in determining response to treatment. This involves a series of interconnected changes in the cellular landscape, spatial organization, and extracellular matrix composition. However, assessing these alterations simultaneously is challenging from a spatial perspective, due to the limitations of current high-dimensional imaging techniques and the extent of intratumoral heterogeneity over large lesion areas. In this study, we introduce a spatial proteomic workflow termed Hyperplexed Immunofluorescence Imaging (HIFI) that overcomes these limi

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"Increasing the Throughput of Activity-Based Proteome Profiling Studies with Real-Time Search and Sample Hyperplexing." Molecular & Cellular Proteomics 21, no. 8 (2022): 100326. http://dx.doi.org/10.1016/j.mcpro.2022.100326.

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Bowser, Bailey L., and Renã A. S. Robinson. "Enhanced Multiplexing Technology for Proteomics." Annual Review of Analytical Chemistry 16, no. 1 (2023). http://dx.doi.org/10.1146/annurev-anchem-091622-092353.

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The identification of thousands of proteins and their relative levels of expression has furthered understanding of biological processes and disease and stimulated new systems biology hypotheses. Quantitative proteomics workflows that rely on analytical assays such as mass spectrometry have facilitated high-throughput measurements of proteins partially due to multiplexing. Multiplexing allows proteome differences across multiple samples to be measured simultaneously, resulting in more accurate quantitation, increased statistical robustness, reduced analysis times, and lower experimental costs.

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Budayeva, Hanna G., Taylur P. Ma, Shuai Wang, Meena Choi, and Christopher M. Rose. "Increasing the Throughput and Reproducibility of Activity-Based Proteome Profiling Studies with Hyperplexing and Intelligent Data Acquisition." Journal of Proteome Research, January 22, 2024. http://dx.doi.org/10.1021/acs.jproteome.3c00598.

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Chong, Li Yen, Felicia Wee, Craig Ryan Joseph, et al. "Automated Hyperplex Protein Staining and Imaging System on Lung Adenocarcinoma Tissue using off-the-shelf Primary Antibodies." World Scientific Annual Review of Cancer Immunology 01 (January 2024). https://doi.org/10.1142/s2972389224300020.

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Furia, Laura, Simone Pelicci, Federica Perillo, et al. "Automated multimodal fluorescence microscopy for hyperplex spatial-proteomics: Coupling microfluidic-based immunofluorescence to high resolution, high sensitivity, three-dimensional analysis of histological slides." Frontiers in Oncology 12 (October 13, 2022). http://dx.doi.org/10.3389/fonc.2022.960734.

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In situ multiplexing analysis and in situ transcriptomics are now providing revolutionary tools to achieve the comprehension of the molecular basis of cancer and to progress towards personalized medicine to fight the disease. The complexity of these tasks requires a continuous interplay among different technologies during all the phases of the experimental procedures. New tools are thus needed and their characterization in terms of performances and limits is mandatory to reach the best resolution and sensitivity. We propose here a new experimental pipeline to obtain an optimized costs-to-benef

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Soares, Ruben R. G., Javier Edo Varg, Attila Szabó, et al. "Hyperplex PCR enables highly multiplexed analysis of point mutations in wastewater: long-term SARS-CoV-2 variant surveillance in Sweden as a case study." Water Research, January 2025, 123154. https://doi.org/10.1016/j.watres.2025.123154.

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Bastiancich, Chiara, Emmanuel Snacel-Fazy, Samantha Fernandez, et al. "Tailoring glioblastoma treatment based on longitudinal analysis of post-surgical tumor microenvironment." Journal of Experimental & Clinical Cancer Research 43, no. 1 (2024). http://dx.doi.org/10.1186/s13046-024-03231-4.

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AbstractGlioblastoma (GBM), an incurable primary brain tumor, typically requires surgical intervention followed by chemoradiation; however, recurrences remain fatal. Our previous work demonstrated that a nanomedicine hydrogel (GemC12-LNC) delays recurrence when administered post-surgery. However, tumor debulking also triggers time-dependent immune reactions that promote recurrence at the resection cavity borders. We hypothesized that combining the hydrogel with an immunomodulatory drug could enhance therapeutic outcomes. A thorough characterization of the post-surgical microenvironment (SMe) i

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Tang, S., E. Bridges, J. Davies, et al. "O-132 A systematic spatial HiPlex proteomics exploration of human testicular tissues from birth to adulthood." Human Reproduction 39, Supplement_1 (2024). http://dx.doi.org/10.1093/humrep/deae108.151.

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Abstract Study question Can we map the key maturation stages of human spermatogonial stem cells (SSCs) within their niche during infancy and puberty? Summary answer Using highplex spatial proteomics, we describe the key cellular events taking place during human testis maturation. What is known already The human testis is a complex “ecosystem” that matures in response to endocrine and exocrine signals and interactions between its diverse cellular components. Unlike animal models, in humans, testicular maturation is a slow process that takes place over more than a decade. In contrast to ovaries,

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