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Relevant bibliographies by topics / Hyphenated HPLC

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Academic literature on the topic 'Hyphenated HPLC'

Author: Grafiati

Published: 5 June 2025

Last updated: 25 June 2025

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Journal articles on the topic "Hyphenated HPLC"

1

S, Thangadurai, B. Sithi Asma, A. Palanimurugan, and A. Cyril. "A Forensic Appraisal of Neoteric Hyphenated Techniques for Separation and Detection of Narcotic Drugs." International Journal of Forensic Sciences 10, no. 1 (2025): 1–17. https://doi.org/10.23880/ijfsc-16000430.

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Narcotic drugs are traditionally been identified on basis of chromatographic-hyphenated techniques in instrumental analysis. Nowadays, contemporary neoteric chromatographic hyphenated techniques have received ever-increasing attention because the principal means to unravel complex analytical problems. A legal examination is a term articulated for examination of all sorts of violations that leads to the prove for any sort of wrong doing as a conclusion of the suspect. Forensic laboratories drugs are identified and separated by TLC, HPLC, HPTLC, GC, LC and known hyphenated techniques which are like HPLC-MS, HPTLC-MS, GC-MS, and LC-MS-MS etc. give reliable and confirmatory leads to drugs identification. HPLC-MS is more preferred method for separation and detection of narcotic drugs. The drawback of HPLC which it requires more solvent as compared to HPTLC. These techniques provide efficient, quick and straightforward method for detection and separation of narcotic drugs and psychotropic substances (NDPS). Analytical chemists face a challenging task in separating, identifying, and determining illicit drugs in pills and biological fluids. There is an increasing need to create advanced methodologies to reduce analysis time, improve sensitivity, and progress towards green chemistry. The hyphenated techniques help in low level detection, highly specific detection, and precise and accurate results for the forensic investigations.

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Komal, Zade* Mrunali Ahirrao Madhuri Shirsath Tufail Dana M. B. Nikam. "HPLC and its Hyphenated Techniques: Review." International Journal of Scientific Research and Technology 2, no. 5 (2025): 584–99. https://doi.org/10.5281/zenodo.15529234.

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High Performance Liquid Chromatography (HPLC) is a precise analytical technique used to separate, identify, and quantify components within complex mixtures. Developed in the late 1960s, it operates by pumping a liquid mobile phase under high pressure (50–350 bars) through a column packed with solid adsorbents like silica or polymers, which interact differently with each sample component to achieve separation. HPLC is favored in pharmaceuticals, biotechnology, environmental testing, and food industries for its speed, sensitivity, and high resolution. Hyphenated techniques in liquid chromatography combine chromatography with spectroscopic methods, a concept introduced by Hirsch Feld in 1980. These integrated systems, such as LC-MS, LC-NMR, and LC-FTIR, use appropriate interfaces to enable simultaneous separation and detailed detection, offering enhanced qualitative and quantitative analysis. These approaches are crucial in modern drug discovery and chemical analysis, providing comprehensive data in a single run.

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Liu, Zhifu, Zhi Xing, Zhaoyang Li, et al. "The online coupling of high performance liquid chromatography with atomic fluorescence spectrometry based on dielectric barrier discharge induced chemical vapor generation for the speciation of mercury." Journal of Analytical Atomic Spectrometry 32, no. 3 (2017): 678–85. http://dx.doi.org/10.1039/c6ja00421k.

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Londonio, Agustín, Paul Emir Hasuoka, Pablo Pacheco, Raúl Andrés Gil, and Patricia Smichowski. "Online solid phase extraction-HPLC-ICP-MS system for mercury and methylmercury preconcentration using functionalised carbon nanotubes for their determination in dietary supplements." Journal of Analytical Atomic Spectrometry 33, no. 10 (2018): 1737–44. http://dx.doi.org/10.1039/c8ja00188j.

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Chindaphan, Kanokporn, Isaya Thaveesangsakulthai, Suchapa Naranaruemol, et al. "Miniaturized electrocoagulation approach for removal of polymeric pigments and selective analysis of non- and mono-hydroxylated phenolic acids in wine with HPLC-UV." RSC Advances 11, no. 11 (2021): 5885–93. http://dx.doi.org/10.1039/d0ra09089a.

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Electrocoagulation (EC) approach was developed to allow fast sample clean-up step prior to selective analysis of non- and mono-hydroxylated phenolic acids in red wine samples with high performance liquid chromatography hyphenated with UV detection (HPLC-UV).

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Ambarsing, P. Rajput, and P. Edlabadkar Aboli. "An Inclusive Review on Analytical Methods for Ritonavir in Various Pharmaceutical and Biological Matrix." Pharmaceutical Methods 8, no. 2 (2017): 01–11. https://doi.org/10.5281/zenodo.14854936.

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Background: Ritonavir (RTV) is an anti-retroviral drug used in the treatment of HIV/ AIDS. Many times, RTV is used alone and in combination with many anti retroviral drugs. So far, around seventy nine analytical methods have been reported for various studies on analysis of RTV in bulk, pharmaceutical formulations and biological fluids. Aim: To summarize the various analytical techniques such as Chromatography, Spectrophotometry; Capillary electrophoresis and also hyphenated techniques such as LC- MS/MS for estimation of Ritonavir. Method: The present article deals with the comprehensive details about the type of various analytical techniques such as Chromatography, Spectrophotometry; Capillary electrophoresis and also hyphenated techniques such as LC- MS/MS, and their applicability in analysis of RTV. These techniques are either explored for the quantification, detection of metabolite and also for stability-studies of the RTV. Conclusion: The present studies revealed that HPLC technique along with the spectro scopic have been most widely explored for the analysis. The investigatory review may provide the comprehensive details to the researchers who are working in the area of analytical research of RTV.

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Adigal, Sphurti S., Sulatha V. Bhandary, Nagaraj Hegde, et al. "Correction: Protein profile analysis of tear fluid with hyphenated HPLC-UV LED-induced fluorescence detection for the diagnosis of dry eye syndrome." RSC Advances 13, no. 39 (2023): 27446. http://dx.doi.org/10.1039/d3ra90084c.

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Correction for ‘Protein profile analysis of tear fluid with hyphenated HPLC-UV LED-induced fluorescence detection for the diagnosis of dry eye syndrome’ by Sphurti S. Adigal et al., RSC Adv., 2023, 13, 22559–22568. https://doi.org/10.1039/D3RA04389D.

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Mandal, Badal Kumar, and Yong-Chien Ling. "Analysis of Chlorophylls/Chlorophyllins in Food Products Using HPLC and HPLC-MS Methods." Molecules 28, no. 10 (2023): 4012. http://dx.doi.org/10.3390/molecules28104012.

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Of the different quality parameters of any food commodity or beverage, color is the most important, attractive and choice-affecting sensory factor to consumers and customers. Nowadays, food industries are interested in making the appearance of their food products attractive and interesting in order to appeal to consumers/customers. Natural green colorants have been accepted universally due to their natural appeal as well as their nontoxic nature to consumers. In addition, several food safety issues mean that natural green colorants are preferable to synthetic food colorants, which are mostly unsafe to the consumers but are less costly, more stable, and create more attractive color hues in food processing. Natural colorants are prone to degradation into numerous fragments during food processing, and thereafter, in storage. Although different hyphenated techniques (especially high-performance liquid chromatography (HPLC), LC-MS/HRMS, and LC/MS-MS are extensively used to characterize all these degradants and fragments, some of them are not responsive to any of these techniques, and some substituents in the tetrapyrrole skeleton are insensitive to these characterization tools. Such circumstances warrant an alternative tool to characterize them accurately for risk assessment and legislation purposes. This review summarizes the different degradants of chlorophylls and chlorophyllins under different conditions, their separation and identification using various hyphenated techniques, national legislation regarding them, and the challenges involved in their analysis. Finally, this review proposes that a non-targeted analysis method that combines HPLC and HR-MS assisted by powerful software tools and a large database could be an effective tool to analyze all possible chlorophyll and chlorophyllin-based colorants and degradants in food products in the future.

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Amir, Mohammad, Puneet Narula, and Farzana Bano. "Analytical Techniques for the Analysis of Lopinavir and Ritonavir in Pharmaceutical Dosage Form and Biological Matrices: A Review." Current Pharmaceutical Analysis 18, no. 4 (2022): 380–414. http://dx.doi.org/10.2174/1573412918666211217145200.

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Background: Lopinavir and Ritonavir are protease inhibitor type of anti-retroviral drugs. Both are used for the treatment of HIV/AIDS. This paper reviews many analytical methods for the analysis of LPV and RTV in pharmaceutical formulations (tablet, capsule, syrup, and bulk) and biological fluids (human plasma, serum, cerebrospinal fluid, rat plasma, and human hair). Objective: The study aims to summarize various analytical techniques, such as chromatography and spectrophotometry, and also hyphenated techniques, such as LC-MS/MS and UPLC-MS, for the analysis of Lopinavir and Ritonavir. Method: The review deals with comprehensive details regarding the type of various analytical techniques, such as spectroscopy (UV), chromatography (RP-HPLC, HPTLC, UPLC), and hyphenated techniques, i.e., LC-MS/MS and UPLC-MS, for the analysis of lopinavir and ritonavir. These techniques are either explored for the quantification and detection of metabolite or for stability studies of the LPV and RTV. Conclusion: The studies presented revealed that the HPLC technique along with spectroscopy have been most widely used for the analysis. Out of the developed methods, hyphenated UPLCMS and LC-MS are very sensitive and help in the easy estimation of drugs compared to other techniques. This review may provide comprehensive details to the researchers working in the area of analytical research of LPV and RTV.

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Peng, Sean X. "Hyphenated HPLC-NMR and its applications in drug discovery." Biomedical Chromatography 14, no. 6 (2000): 430–41. http://dx.doi.org/10.1002/1099-0801(200010)14:6<430::aid-bmc32>3.0.co;2-p.

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Dissertations / Theses on the topic "Hyphenated HPLC"

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Timischl, Birgit. "Hyphenated mass spectrometric methods for quantitative metabolomics in E. coli and human cells." kostenfrei, 2008. http://www.opus-bayern.de/uni-regensburg/volltexte/2008/1028/.

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LA, MAIDA NUNZIA. "Development and validation of analytical methods with hyphenated chromatographic techniques for the determination of NPS and drugs of abuse in biological matrices." Doctoral thesis, Università Politecnica delle Marche, 2022. https://hdl.handle.net/11566/299808.

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Sin dal 1990 l'apparizione sul mercato di nuove sostanze psicoattive (NPS) è un fenomeno mondiale sempre crescente. Ogni anno vengono introdotte nuove sostanze nel mercato della droga come alternative legali alle sostanze controllate dalla legge. L’identificazione di queste nuove sostanze in matrici biologiche rappresenta una continua sfida per i laboratori di analisi. È estremamente importante sviluppare metodologie analitiche in grado di identificare sostanze sconosciute consentendone il monitoraggio e fornendo informazioni scientifiche riguardo la farmacocinetica, possibili range di tossicità ed effetti farmacologici. Oggi, le tecniche cromatografiche ifenate (tecniche basate sull’accoppiamento di più strumentazioni) sono dei mezzi molto utilizzati nella tossicologia clinica e forense. Il seguente progetto di ricerca ha lo scopo di sviluppare e validare nuovi metodi analitici basati su tecniche cromatografiche accoppiate alla spettrometria di massa e spettrometria di massa tandem per la determinazione delle NPS e delle classiche droghe d’abuso in matrici biologiche. In particolare, considerando la poliassunzione di droghe, è stato sviluppato un metodo di screening utilizzando la cromatografia liquida ad alta prestazione abbinata alla spettrometria di massa tandem (HPLC-MS-MS) per la determinazione di 77 NPS (appartenenti a diverse classi di sostanze), 24 droghe classiche e 18 metaboliti nel sangue, in urina e saliva. Attualmente, i cannabinoidi sintetici sono il più grande gruppo di NPS monitorato dall’Agenzia Europea per le Droghe (EMCDDA ). Due nuovi metodi analitici sono stati sviluppati per la determinazione dei cannabinoidi sintetici JWH-122, JWH-210 e UR-144 in saliva: un metodo in gascromatografia accoppiata alla spettrometria di massa (GC-MS) e un metodo complementare che utilizza la cromatografia liquida ad alta prestazione abbinata alla spettrometria di massa ad alta risoluzione (UHPLC-HRMS) per la quantificazione sia dei parent drugs che dei loro metaboliti. Infine, in risposta alla crescente diffusione di nuovi catinoni sintetici, viene presentato un metodo mirato per la loro identificazione e quantificazione mediante cromatografia liquida ad alte prestazioni e spettrometria di massa ad alta risoluzione (UHPLC-HRMS). Tutti i metodi qui descritti sono stati validati con successo e applicati a campioni reali (sangue, urina, saliva, capelli) fornendo risultati affidabili e dimostrando di essere metodologie sensibili utilizzabili in laboratori clinici e forensi.<br>The emergence of new psychoactive substances (NPS) has been a growing global phenomenon since 1990. Every year new substances appear into the drug market as legal alternatives to illicit drugs. In this regard, the identification in biological matrices of emerging synthetic compounds represents a continuous challenge for analytical toxicologists. It is extremely important to develop suitable drugs analysis methodologies which can provide information on pharmacokinetics, pharmacodynamics effects and possible toxic concentration ranges, and on the diffusion of these unknown substances. Hyphenated chromatographic techniques have become powerful tools largely used in clinical and forensic toxicology. This research project aimed to develop and validate new analytical methodologies based on hyphenated chromatographic techniques for the determination of NPS and classical drugs of abuse in biological matrices. Considering the polydrug consumption, a comprehensive high-performance liquid chromatography–tandem mass spectrometry (HPLC–MS-MS) screening method was developed for the determination of 77 NPS (belonging to different chemical classes), 24 classic drugs and 18 related metabolites in blood, urine, and oral fluid. Currently, synthetic cannabinoids are the largest group of NPS monitored by the European Monitoring Centre for Drugs and Drug Addiction and to investigate the oral fluid pharmacokinetics of JWH-122, JWH-210 and UR-144 two new methodologies were developed. A gas chromatography-mass spectrometry (GC-MS) method for the determination of parent compounds and a complementary ultra-high performance liquid chromatography high resolution mass spectrometry (UHPLC-HRMS) confirmatory method for the quantification also of their metabolites. Finally, to keep in pace with the increasing spread of new synthetic cathinones an ultra-high performance liquid chromatography high resolution mass spectrometry (UHPLC-HRMS) method is presented for their simultaneous targeted screening and quantification in hair samples of consumers. All methods described here have been successfully validated and applied to authentic samples (blood, urine, oral fluid, hair) providing reliable results and proving to be sensitive methodologies suitable for use in clinical and forensic laboratories.

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Wang, Tongwen. "Hyphenated HPLC-MS technique for analysis of compositional monosaccharides of transgenic corn glycoprotein and characterization of degradation products of diazinon, fonofos and aldicarb in various oxidation systems." Diss., Rolla, Mo. : University of Missouri-Rolla, 2007. http://scholarsmine.mst.edu/thesis/pdf/WangTongwen_09007dcc804e975c.pdf.

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Thesis (Ph. D.)--University of Missouri--Rolla, 2007.<br>Vita. The entire thesis text is included in file. Title from title screen of thesis/dissertation PDF file (viewed April 23, 2008) Includes bibliographical references.

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Qvarnström, Johanna. "On the reliability of methods for the speciation of mercury based on chromatographic separation coupled to atomic spectrometric detection." Doctoral thesis, Umeå universitet, Kemi, 2003. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-111.

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This thesis deals with the reliability of methods for the speciation of mercury in environmental and biological samples. Problems with speciation methods that couple chromatography to atomic spectrometric detection and how to overcome the problems are discussed. Analytical techniques primarily studied and evaluated are high performance liquid chromatography-cold vapour-atomic absorption spectrometry (HPLC-CV-AAS), HPLC-inductively coupled plasma-mass spectrometry (HPLC-ICP-MS), capillary electrophoresis-ICP-MS (CE-ICP-MS) and gas chromatography-ICP-MS (GC-ICP-MS). Applying a multi-capillary approach increased the analyte amount injected into a CE-ICP-MS system and improved the overall sensitivity. A microconcentric nebulizer with a cyclone spray chamber was shown to improve the detection limits for mercury species 3-13 times in HPLC-ICP-MS and 11-19 times in CE-ICP-MS compared to a cross-flow nebulizer with a Scott spray chamber. To decrease the interference of water vapour in HPLC-CV-AAS a Nafion dryer tube was inserted between the CV-generation and the detector. Methyl mercury was however lost in the Nafion unless it was reduced to elemental mercury prior transport through the dryer tube. During sample pre-treatment, incomplete extraction, losses and transformation (alkylation, dealkylation, oxidation and reduction) of mercury species can lead to significant errors (underestimation and overestimation) in the determination of the concentrations. Methods to detect and determine the degree of transformation as well as correct for errors caused by transformation are presented in the thesis. The preferable method use species-specific enriched stable isotope standards in combination with MS detection and a matrix based calculation scheme. This approach is very powerful as both the concentrations of the species as well as the degrees of transformation can be determined within each individual sample.

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Mnatsakanyan, Mariam. "Natural antioxidants and high performance liquid chromatography (HPLC) hyphenated screening techniques." Thesis, 2010. http://handle.uws.edu.au:8081/1959.7/496789.

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Reactive intermediates in the oxidative processes are among the major sources of primary catalysts that initiate oxidation in vivo and in vitro; a process that is one of the major causes of food quality deterioration and generation of chronic diseases. Antioxidants, both natural and synthetic, have been widely used at legal limits in food, medical and personal care industries. However, recently, the use of synthetic antioxidants has been severely restricted because of the evidence that suggests they may be harmful to human health. As such, the importance of screening naturally occurring alternatives, which are presumably safe, effective as dietary supplements or as processing aids, is increasing. Natural products are well known for their molecular biodiversity and are of great interest as potential source for novel antioxidant molecules. Due to the chemical complexity of natural products, determining their antioxidant content can be a formidable task, but at the same time likely to be worthwhile task. This thesis investigates the hyphenation of one- and multi-dimensional chromatography towards to profiling antioxidant content in natural products.

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陳寬孺. "Development of HPLC-UV/nano-TiO2-ICP-MS hyphenated system for the determination of mercury species in urine sample." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/76794108859720529861.

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碩士<br>國立清華大學<br>生醫工程與環境科學系<br>94<br>Abstract With elemental, inorganic and organometallic mercury each having different chemical and physical properties, routes of exposure, and toxicological effects. The analysis of mercury species has proven to be critical in assessing their direct influence on the environment, predicting their transport behavior in several ecosystem compartments and in the decontamination of hazardous pollutant materials. Although considerable efforts have been made in recent years for mercury speciation analysis, the analytical methodology still needs further improvement to promote the analytical sensitivity of detection. In order to meet the request for investigating biological significance of mercury species, in this study, Hg2+ and CH3Hg+ were separated using a cation-exchange chromatography column packed with polyhydroxy methacrylate gel. After the chromatographic separation, on-line transformation of mercury species into gaseous products by UV/nano-TiO2 photocatalysis reduction device was investigated to enhance the sensitivity of detection and reduce the non-spectral interference resulted from sample matrix. In order to enhance the vaporization efficiency, the effects of acidity, amount of TiO2 and formic acid on the vaporization efficiency of Hg2+ and CH3Hg+ were investigated. Based on the experimental results, 100 mM formic acid and 3 g/L nano-TiO2 suspension (Degussa p25) were used to on-line vaporize two mercury species under UV illumination. With our proposed procedure, Hg2+ and CH3Hg+ in the column effluent can be on-line vaporized and determined by ICP-MS after the chromatographic separation without the need of conventional chemical vaporization method.

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吳俊甫. "Development of online HPLC-PMMA Chip-based Photo-Catalyst Reduction Device (PC2RD)-ICP-MS hyphenated system for determination of selenium species in nature water." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/49617898272767421161.

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碩士<br>國立清華大學<br>生醫工程與環境科學系<br>96<br>Recently, speciation of selenium has attracted wide attention not only because the narrow threshold concentration range between deficiency and toxicity, but also its complexity in phisco-chemical form presenting in various environment and biological media. In general, the major soluble forms of selenium in nature waters have been proven to be selenite and selenate. The concentrations of selenium species in nature waters are extremely low; therefore, it is of great important to develop a sensitive and selective method for the study of the fate of selenium species in aqueous environment. According to literatures, undoubtedly, HPLC-ICP-MS hyphenated technique is one of the most sensitive method for the determination of trace selenium species. However, the analytical sensitivity of ICP-MS for selenium is highly limited by the high ionization energy (9.75 eV) of selenium and the inferior transport efficiency (mostly 2–3%) provided by traditional sample introduction systems. To compensate for the inferiority of HPLC-ICP-MS system, hydride generation (HG) technique has been successfully used to interface chromatography and spectrometers by enhancing analyte transportation efficiency and sensitivity. Recently, a novel photo-catalyst reduction device (PCRD) was developed, which has been verified as an efficient and high-performing technique for selenite and selenate detection. Because the PCRD was constructed by a PTFE tube, in this study, we intended to develop a PMMA (polymethylmethacrylate) Chip-base photo-catalyst reduction device (PC2RD) to improve the light transmission and sensitivity of detection. After the fabrication of meso-fluidic chip by CO2-Laser engraver, a UV lamp with 365 nm wavelength was used for the irradiation. Based on the experimental results, PC2RD system could improve the transport efficiency of analyte selenium species by 20%, comparing to PCRD system. Moreover, it merely needed 29s to transform inorganic selenium into gaseous selenide. The limit of detection of Se(Ⅳ) and Se(Ⅵ) were 4.9 and 3.9 ng/L, respectively. Based on the achieved analytical results, it indicated that our developed HPLC-PC2RD-ICP-MS system is a fast, simple and high sample transportation efficiency method, and has the ability to determine different selenium species in natural waters.

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Yu-Cheng, Chang, and 張育誠. "Development of online HPLC-UV/nano-TiO2 /HCOOH PCRD-ICP-MS hyphenated system for batch determination of selenium species in natural water and continuous monitoring of dynamic variation of selenium species in urine sample." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/73501254725566991205.

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碩士<br>國立清華大學<br>原子科學系<br>93<br>The essential trace mineral, selenium, is of fundamental importance to human health. As a constituent of selenoproteins, selenium has structural and enzymic roles. According to literatures, the bioavailability and toxicity of selenium is highly dependent on its chemical forms and their concentration. Thus, it is very important to quantitate different chemical species in the environmental and biological samples. Up to now, chemical hydridization methods are frequently employed to interface chromatographic separation and instrument detection. In view of the difficulty in converting Se(VI) into gaseous hydride by chemical methods, a new vapor generation technique utilizing HPLC-UV/nano-TiO2/HCOOH photocatalysis redox device coupled with ICP-MS for the speciation of selenium species was developed. In this study, selenite (Se(IV)) and selenate(Se(VI)), were separated using anion-exchange chromatography column packed with Nucleosil 100-5 SB under the elution of 50 mM carbonate buffer. After the chromatographic separation, on-line pre-redox of selenium species into gaseous hydrides by UV/nano-TiO2/HCOOH PCRD （photocatalysis redox device）were investigated to enhance the sensitivity of detection and reduce the non-spectral interference resulted from sample matrix. In order to enhance the hydridization efficiency, the effects of acidity, amount of TiO2 and formic acid and the reaction coil length were investigated. By way of the use of proposed procedure, Se(IV) and Se(VI) can be on-line determined after the chromatographic separation. Under the optimized condition, similar signal intensity can be reached in the use of the proposed photocatalysis redox hydride generation device. In order to validate the applicability of the established on-line system, the content of analyte species in agriculture irrigation water and human urine samples were measured. By combining with microdialysis sampling system, the feasibility to in vivo, in-situ and continuous monitor the dynamic variation of trace selenium species in urine sample has also been ascertained.

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Book chapters on the topic "Hyphenated HPLC"

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Hesse, Anne-Marie, Sega Ndiaye, and Joelle Vinh. "Reversed-Phase HPLC and Hyphenated Analytical Strategies for Peptidomics." In Methods in Molecular Biology. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-310-3_13.

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Hopfgartner, Gérard, and Emmanuel Varesio. "Tandem Mass Spectrometry Hyphenated with HPLC and UHPLC for Targeted Metabolomics." In Metabolomics in Practice. Wiley-VCH Verlag GmbH & Co. KGaA, 2013. http://dx.doi.org/10.1002/9783527655861.ch2.

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Tuzimski, Tomasz. "Basic Principles of Planar Chromatography and Its Potential for Hyphenated Techniques." In High-Performance Thin-Layer Chromatography (HPTLC). Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-14025-9_14.

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Cimpoiu, Claudia. "HPTLC Hyphenated with FTIR: Principles, Instrumentation and Qualitative Analysis and Quantitation." In High-Performance Thin-Layer Chromatography (HPTLC). Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-14025-9_18.

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"HPLC-Hyphenated Techniques." In Analytical Instrumentation Handbook. CRC Press, 2004. http://dx.doi.org/10.1201/9780849390395-32.

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Shalliker, R., and M. Gray. "HPLC-Hyphenated Techniques." In Analytical Instrumentation Handbook, Second Edition. CRC Press, 2004. http://dx.doi.org/10.1201/9780849390395.ch29.

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Kusu, Fumiyo, and Akira Kotani. "HPLC with Electrochemical Detection." In Hyphenated and Alternative Methods of Detection in Chromatography. CRC Press, 2011. http://dx.doi.org/10.1201/b11283-7.

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Chapman, John R. "Mass spectrometric detectors." In HPLC of Macromolecules. Oxford University PressOxford, 1998. http://dx.doi.org/10.1093/oso/9780199635719.003.0002.

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Abstract Mass spectrometry (MS) is one of the most powerful physical methods available for identifying analytes in terms of their molecular mass (Mr) and for elucidating their molecular structures. Furthermore, MS possesses a unique attribute compared to other spectroscopies, namely its high sensitivity, so that it provides one of the few methods that is entirely suitable for the identification, structural elucidation, and for the quantitative measurement of even trace amounts of the various analytes present in a mixture. Generally, however, and certainly for complex mixtures, by combining MS with a chromatographic technique the molecular identity of each of the separated components of a mixture may be more readily ascertained. Historically, the first combination of MS with a separation technique was that achieved with gas chromatography, namely GC-MS, but as will become evident from the present chapter, during the last decade this desirable practice has been extended to HPLC to give the hyphenated technique usually designated as LC-MS.

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Francis, Paul S., and Jacqui L. Adcock. "Liquid-Phase Chemiluminescence Detection for HPLC." In Hyphenated and Alternative Methods of Detection in Chromatography. CRC Press, 2011. http://dx.doi.org/10.1201/b11283-8.

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Potey, Lata, Ashwini Armarkar, Shilpa Chapekar, Yogini Shete, Pooja Birade, and Suchita Waghmare. "APPLICATIONS OF HYPHENATED TECHNIQUES IN PHARMACEUTICAL SCIENCES." In Futuristic Trends in Chemical Material Sciences & Nano Technology Volume 3 Book 18. Iterative International Publishers, Selfypage Developers Pvt Ltd, 2024. http://dx.doi.org/10.58532/v3bdcs18ch19.

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The hyphenation term was introduced by Thomas Hirschfield in 1980. Hyphenated techniques are exponentially to deal with various complicated challenges faced by analyst in analytical chemistry. It is the fusion of chromatographic methods and spectrometric or spectroscopic methods. Chromatographic methods are separation methods which separate component in the complex mixture like gas chromatography (GC), liquid chromatography (LC), high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE). Spectroscopy use to elucidate the structure, molecular mass of compounds like UV, IR, Mass, Raman, Fluroescence spectroscopy. The remarkable improvements in hyphenated analytical methods over the last two decades have significantly broadened their applications in the analysis of biomaterials, especially natural products. Nowadays, various types of hyphenated techniques incorporating different types of interfaces are available commercially to show better analysis of the samples is components specificity, accuracy, precision. In this chapter, we have emphasized on most common types of double and triple hyphenated techniques like GC-MS, LC-MS, LC-FTIR, LC-NMR, LC-NMR-MS, etc and their applications in the field of pharmaceutical sciences.

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Conference papers on the topic "Hyphenated HPLC"

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Guo, Zhongxian, Qiantao Cai, and Zhaoguang Yang. "Determination of Water-Soluble Organophosphorus Herbicides by Ion Chromatography With Inductively Coupled Plasma Mass Spectrometry Detection." In 1st Water Quality, Drought, Human Health and Engineering Conference. ASMEDC, 2006. http://dx.doi.org/10.1115/water2006-20024.

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Abstract:

There is a high risk for human exposure to organophosphorus pesticides through contaminated drinking water. Glyphosate, glufosinate, fosamine and ethephon are among the water-soluble herbicides used currently. Sensitive and rapid analytical methodologies are critical for evaluating their residuals in a broad variety of samples, including environmental waters. However, challenges arise from the inherent chemical properties of the herbicides: strong polarity, high solubility in water, insolubility in organic solvent (except ethephon), absence of chromophore or fluorophore in their molecular structures. So far very rare analytical methods are available for ethephon [1] and fosamine [2], while glyphosate and glufosinate are often determined by gas chromatography [3], high performance liquid chromatography (HPLC) [4] and capillary electrophoresis (CE) [5]. Inductively coupled plasma mass spectrometry (ICP-MS) is sensitive, rapid, selective, and is more powerful when hyphenated with appropriate separation. For the analysis of glufosinate, glyphosate and its metabolite aminomethylphosphonic acid (AMPA), ICP-MS was recently coupled to CE [6] or ion-pairing reversed-phase LC [7].

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