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Journal articles on the topic 'Hypothermic Storage'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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1

Holovina, K. M., O. M. Bobrova, S. Y. Kovalenko, Y. S. Hovorova, and O. A. Nardid. "Effect of ozonation on resistance of ovine and human erythrocytes to hypothermic storage." Regulatory Mechanisms in Biosystems 12, no. 1 (2021): 116–20. http://dx.doi.org/10.15421/022118.

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Long-term hypothermic storage of animal blood can lead to the loss of its quality and can cause complications in recipient animals after transfusion, so the search for new methods of increasing the preservation of erythrocytes after hypothermic storage continues. The article presents the data of the ozonation effect on the preservation rate of ovine and human erythrocytes during hypothermic storage with Alsever’s solution and mannitol medium. Hemolysis, osmotic fragility and distribution density of ovine and human erythrocytes by the sphericity index were determined at different stages of hypo

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2

Chen, Qun, Amadou K. S. Camara, Jianzhong An, Matthias L. Riess, Enis Novalija, and David F. Stowe. "Cardiac preconditioning with 4-h, 17°C ischemia reduces [Ca2+]i load and damage in part via KATP channel opening." American Journal of Physiology-Heart and Circulatory Physiology 282, no. 6 (2002): H1961—H1969. http://dx.doi.org/10.1152/ajpheart.01032.2001.

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Brief ischemia before normothermic ischemia protects hearts against reperfusion injury (ischemic preconditioning, IPC), but it is unclear whether it protects against long-term moderate hypothermic ischemia. We explored in isolated guinea pig hearts 1) the influence of two 2-min periods of normothermic ischemia before 4 h, 17°C hypothermic ischemia on cardiac cytosolic [Ca2+], mechanical and metabolic function, and infarct size, and 2) the potential role of KATP channels in eliciting cardioprotection. We found that IPC before 4 h moderate hypothermia improved myocardial perfusion, contractility

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Isaev, Dmitry. "Hypothermic storage of sturgeon sperm: methodology and ongoing history." Rybovodstvo i rybnoe hozjajstvo (Fish Breeding and Fisheries), no. 8 (August 1, 2020): 64–82. http://dx.doi.org/10.33920/sel-09-2008-06.

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Hypothermic storage of sperm in a liquid state without freezing, without the use of either liquid nitrogen or dry ice as well as special cryological equipment is an interesting and attractive research line in reproductive biology in terms of practical application. Historically, hypothermia is the very first approach to the preservation of genetic material, but, despite this, the methods of hypothermic storage of gametes and embryos have not received proper development and application in animal husbandry, giving way to cryopreservation. One of the main reasons for this is the high species-speci

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4

Bailey, Leslie E. "Preservation of guinea pig hearts by hypothermic gas perfusion." Canadian Journal of Physiology and Pharmacology 67, no. 7 (1989): 692–96. http://dx.doi.org/10.1139/y89-112.

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The lack of a satisfactory method for long-term preservation of hearts during transport limits the source of human hearts for transplant to the geographic vicinity of the transplant center. Experimentally, reduction of myocardial oxygen requirements with hypothermia and cardioplegia prolong storage time to 48 h, but always with some evidence of myocardial damage. In this study, the combination of hypothermia with a procedure known to increase oxygen tension in cardiac muscle, gas perfusion, preserved contractile activity in guinea pig hearts for 24 h and did not cause edema. Cardioplegia or ga

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5

Golovina, Ksenia, Olena Bobrova, and Svitlana Kovalenko. "Hypothermic storage of ovine erythrocytes." Problems of Cryobiology and Cryomedicine 30, no. 3 (2020): 288. http://dx.doi.org/10.15407/cryo30.03.288.

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6

PONZIN, D. "Hypothermic storage or organ culture?" Acta Ophthalmologica 91 (August 2013): 0. http://dx.doi.org/10.1111/j.1755-3768.2013.2671.x.

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7

See, Yew P., Richard D. Weisel, Donald A. G. Mickle, et al. "Prolonged hypothermic cardiac storage for transplantation." Journal of Thoracic and Cardiovascular Surgery 104, no. 3 (1992): 817–24. http://dx.doi.org/10.1016/s0022-5223(19)34755-5.

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8

Snyder, Kristi K., John M. Baust, Robert G. Van Buskirk, and John G. Baust. "Enhanced Hypothermic Storage of Neonatal Cardiomyocytes." Cell Preservation Technology 3, no. 1 (2005): 61–74. http://dx.doi.org/10.1089/cpt.2005.3.61.

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MALININ, THEODORE I., JOSEPH L. WAGNER, JULIO C. PITA, and HILDA LO. "Hypothermic Storage and Cryopreservation of Cartilage." Clinical Orthopaedics and Related Research &NA;, no. 197 (1985): 15???26. http://dx.doi.org/10.1097/00003086-198507000-00004.

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10

Lau, Nicola, Aida Hajjar Sesé, Victor A. Augustin, et al. "Fungal infection after endothelial keratoplasty: association with hypothermic corneal storage." British Journal of Ophthalmology 103, no. 10 (2018): 1487–90. http://dx.doi.org/10.1136/bjophthalmol-2018-312709.

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PurposeTo compare the incidence of fungal infection after endothelial keratoplasty (EK) when donor tissue had been stored in hypothermic medium or organ culture.MethodsWe describe the clinical features of 10 cases of fungal infection (keratitis or endophthalmitis) following EK identified at three European centres. Case definition was the culture of fungus or a positive PCR from the host cornea or anterior chamber after EK. A survey of the incidence of infection after EK was conducted by the European Eye Bank Association. The main outcome measure was the number of cases in which donor tissue ha

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11

Petrenko, Yuriy, Milada Chudickova, Irena Vackova, et al. "Clinically Relevant Solution for the Hypothermic Storage and Transportation of Human Multipotent Mesenchymal Stromal Cells." Stem Cells International 2019 (January 20, 2019): 1–11. http://dx.doi.org/10.1155/2019/5909524.

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The wide use of human multipotent mesenchymal stromal cells (MSCs) in clinical trials requires a full-scale safety and identity evaluation of the cellular product and subsequent transportation between research/medical centres. This necessitates the prolonged hypothermic storage of cells prior to application. The development of new, nontoxic, and efficient media, providing high viability and well-preserved therapeutic properties of MSCs during hypothermic storage, is highly relevant for a successful clinical outcome. In this study, a simple and effective trehalose-based solution was developed f

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Finegan, Barry A., Manoj Gandhi, Matthew R. Cohen, Donald Legatt, and Alexander S. Clanachan. "Isoflurane Alters Energy Substrate Metabolism to Preserve Mechanical Function in Isolated Rat Hearts following Prolonged No-Flow Hypothermic Storage." Anesthesiology 98, no. 2 (2003): 379–86. http://dx.doi.org/10.1097/00000542-200302000-00018.

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Background Isoflurane enhances mechanical function in hearts subject to normothermic global or regional ischemia. The authors examined the effectiveness of isoflurane in preserving mechanical function in hearts subjected to cardioplegic arrest and prolonged hypothermic no-flow storage. The role of isoflurane in altering myocardial glucose metabolism during storage and reperfusion during these conditions and the contribution of adenosine triphosphate-sensitive potassium (K(atp)) channel activation in mediating the functional and metabolic effects of isoflurane preconditioning was determined. Me

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Gramignoli, Roberto, Kenneth Dorko, Veysel Tahan, et al. "Hypothermic Storage of Human Hepatocytes for Transplantation." Cell Transplantation 23, no. 9 (2014): 1143–51. http://dx.doi.org/10.3727/096368913x668627.

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14

Almizraq, Ruqayyah J., Jayme D. R. Tchir, Jelena L. Holovati, and Jason P. Acker. "Red blood cell microvesiculation during hypothermic storage." Cryobiology 63, no. 3 (2011): 311. http://dx.doi.org/10.1016/j.cryobiol.2011.09.024.

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15

Orita, Hiroyuki, Manabu Fukasawa, Hideaki Uchino, Kana Fukui, Minoru Kohi, and Masahiko Washio. "Modulation of the viability of immature cardiac myocytes by cardiac fibroblasts after hypothermic preservation—its values as a technique for evaluation of storage solutions." Cardiology in the Young 5, no. 2 (1995): 110–17. http://dx.doi.org/10.1017/s1047951100011665.

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AbstractWe evaluated the modulation of the viability of immature cardiac myocytes by cardiac fibroblasts after hypothermic preservation using three types of storage solutions—saline, University of Wisconsin solution, and MCDB 107 medium. Cardiac myocytes and fibroblasts were isolated from neonatal rat ventricles, and cultures of myocytes only or co-cultures with fibroblasts (myocyte: fibroblast 2:1) were established. On the fourth day of culture, the cultures were incubated at 4 °C for 6, 12, 18 and 24 hours in the different storage solutions. Enzymes were measured in the storage solutions imm

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16

Hidalgo, M. A., D. J. Mann, B. J. Fuller, and C. J. Green. "Effects of Depolarizing or Non-Depolarizing Preservation Solutions on Human Endothelial cells during Cold Hypoxia." Clinical Science 90, no. 2 (1996): 135–41. http://dx.doi.org/10.1042/cs0900135.

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1. Hypothermic storage of whole organs flushed with a preservation solution is common practice in clinical transplantation. This procedure leaves vascular endothelial cells in direct contact with the preservation solution during the length of the cold ischaemic period. 2. Aiming to study the effects of organ preservation on vascular endothelium, we subjected cultures of human umbilical vein endothelial cells to hypoxic and hypothermic storage conditions in vitro for 3 or 16 h. Four preservation solutions with different levels of sodium and potassium were tested. Morphometric analysis and 51Cr

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Ljubojević, Vesna, Sanja Jovičić, Milka Mavija, and Zoran Vujković. "Efficacy of two different antibiotic solutions in preservation of fresh amniotic membrane." Биомедицинска истраживања 11, no. 1 (2020): 20–28. http://dx.doi.org/10.5937/bii2001020l.

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Introduction. The amniotic membrane is used in transplant surgery, ophthalmology and dermatology. Various methods have been developed to preserve amniotic membrane: hypothermic storage, cryopreservation, lyophilization. Transplantation of fresh amniotic membrane showed low inflammatory response. The efficient antibiotic solutions are carefully chosen for the hypothermic storage of amniotic membranes. The aim of this study was to compare the efficacy of two antibiotic solutions for the hypothermic amniotic membrane preservation and the structure of the amniotic membrane after the preservation p

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18

FAGBEMI, Oluwole S., Kieran BRACK, Surjit GOLAR, David CRISP, and Apollo ECONOMIDES. "Electrophysiological and biochemical changes in rabbit hearts stored at 4 °C for 6 or 24 h." Clinical Science 101, no. 4 (2001): 367–76. http://dx.doi.org/10.1042/cs1010367.

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This study examines the electrophysiological and metabolic changes that occur in rabbit hearts during hypothermic storage in vitro. Hearts were microperfused at 4°C for 6 or 24h with either normal Krebs-Henseleit buffer (KHB) or KHB containing 2,3-butanedione monoxime (BDM). After hypothermic storage, hearts were rewarmed to 37°C with KHB. Cardiac function was then assessed in Langendorff perfusion mode. Electrophysiological changes were also assessed from the ventricular paced-evoked responses. After storage, mitochondria were isolated from the hearts and their respiratory control ratio, rate

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19

Parekh, Mohit, Davide Borroni, Vito Romano, et al. "Next-generation sequencing for the detection of microorganisms present in human donor corneal preservation medium." BMJ Open Ophthalmology 4, no. 1 (2019): e000246. http://dx.doi.org/10.1136/bmjophth-2018-000246.

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ObjectiveTo detect the presence of microorganisms in the storage media of human donor corneas using next-generation sequencing method.MethodsSeven samples from organ culture (OC) group (Cornea Max, Eurobio, Les Ulis, France) with one control (sterile media without any cornea) and seven samples from hypothermic storage group (Cornea Cold, Eurobio) with one control were used for this study. The corneas were placed in the respective storage media for 14 days before collecting the samples. Storage media (2 mL) from each sample were collected in RNAase-free tubes and shipped for ribosomal RNA seque

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20

Abrahamse, Salomon L., Pieter Van Runnard Heimel, Robin J. Hartman, Rob A. F. M. Chamuleau, and Thomas M. Van Gulik. "Induction of Necrosis and DNA Fragmentation during Hypothermic Preservation of Hepatocytes in UW, HTK, and Celsior Solutions." Cell Transplantation 12, no. 1 (2003): 59–68. http://dx.doi.org/10.3727/000000003783985160.

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Donor cells can be preserved in University of Wisconsin (UW), histidine-tryptophan-ketoglutarate (HTK), or Celsior solution. However, differences in efficacy and mode of action in preventing hypothermia-induced cell injury have not been unequivocally clarified. Therefore, we investigated and compared necrotic and apoptotic cell death of freshly isolated primary porcine hepatocytes after hypothermic preservation in UW, HTK, and Celsior solutions and subsequent normothermic culturing. Hepatocytes were isolated from porcine livers, divided in fractions, and hypothermically (4°C) stored in phospha

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21

Rauen, Ursula, and Herbert de Groot. "New Insights into the Cellular and Molecular Mechanisms of Cold Storage Injury." Journal of Investigative Medicine 52, no. 5 (2004): 299–309. http://dx.doi.org/10.1136/jim-52-05-29.

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Solid organ grafts, but also other biologic materials requiring storage for a few hours to a few days, are usually stored under hypothermic conditions. To decrease graft injury during cold storage, organ preservation solutions were developed many years ago. However, since then, modern biochemical and cell biologic methods have allowed further insights into the molecular and cellular mechanisms of cold storage injury, including further insights into alterations of the cellular ion homeostasis, the occurrence of a mitochondrial permeability transition, and the occurrence of free-radical-mediated

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22

Golovina, K., O. Bobrova, O. Shapkina, E. Nipot, and Yu Hovorova. "Decreasing of Erythrocytes Mechanical Resistance during Hypothermic Storage." Ukraïnsʹkij žurnal medicini, bìologìï ta sportu 5, no. 4 (2020): 357–61. http://dx.doi.org/10.26693/jmbs05.04.357.

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23

Guibert, E. "Hypothermic Storage of Periportal and Perivenous Rat Hepatocytes." Cell Transplantation 7, no. 4 (1998): 345–55. http://dx.doi.org/10.1016/s0963-6897(98)00015-3.

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24

M??ller, Christian, Hans Hoffmann, Iris Bittmann, et al. "HYPOTHERMIC STORAGE ALONE IN LUNG PRESERVATION FOR TRANSPLANTATION." Transplantation 63, no. 5 (1997): 625–30. http://dx.doi.org/10.1097/00007890-199703150-00002.

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25

Guibert, Edgardo Elvio, María Gabriela Mediavilla, María Eugenia Mamprin, and Joaquín Valentín Rodríguez. "Hypothermic Storage of Periportal and Perivenous Rat Hepatocytes." Cell Transplantation 7, no. 4 (1998): 345–55. http://dx.doi.org/10.1177/096368979800700402.

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High yields of intact parenchymal cells are produced by the two-step Digitonin-collagenase perfusion of whole liver, and it has gained wide acceptance for biochemical and cellular analyses of zonal hepatocytes. The development reached by this methodology is in contrast to the time-limited use of the isolated cells unless those other methods, such as primary cultures, are employed. An alternative option to have cells ready to be used for several days, is the cold storage in University of Wisconsin solution as a preservation solution. This procedure is easy, not too expensive, and does not requi

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Ries, WP, Y. Marie, K. Patel, et al. "A simple ex vivo model of human renal allograft preservation using the gonadal vein." Annals of The Royal College of Surgeons of England 101, no. 8 (2019): 609–16. http://dx.doi.org/10.1308/rcsann.2019.0107.

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Introduction Hypothermic machine perfusion, an organ preservation modality, involves flow of chilled preservation fluid through an allograft’s vasculature. This study describes a simple, reproducible, human model that allows for interrogation of flow effects during ex vivo organ perfusion. Materials and methods Gonadal veins from deceased human renal allografts were subjected to either static cold storage or hypothermic machine perfusion for up to 24 hours. Caspase-3, Krüppel-like factor 2 expression and electron microscopic analysis were compared between ‘flow’ and ‘no-flow’ conditions, with

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Isaev, Dmitry Alexandrovich, Alexander Pavlovich Glebov, Marina Yurievna Martynova, and Elena Ivanovna Shishanova. "Hypothermic storage in salt-free preservative solution alter motility duration in sterlet sperm." Rybovodstvo i rybnoe hozjajstvo (Fish Breeding and Fisheries), no. 5 (May 1, 2021): 64–79. http://dx.doi.org/10.33920/sel-09-2105-05.

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Together with concentration, motility is one of the most important characteristics of sturgeon sperm, determining its quality and suitability for insemination. After activation in water, the duration of progressive sperm motility is also important, and this time should not be less than that required for fertilization. Motility of spermatozoa depends on their physiological state, maturity, age and intracellular reserves of macroergic substances. During hypothermic storage, the percentage of spermatozoa that can be activated decreases progressively due to depletion of ATP supply or cell death. T

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Pahernik, S. A., W. E. Thasler, J. Mueller-Hoecker, F. W. Schildberg, and H. G. Koebe. "Hypothermic Storage of Pig Hepatocytes: Influence of Different Storage Solutions and Cell Density." Cryobiology 33, no. 5 (1996): 552–66. http://dx.doi.org/10.1006/cryo.1996.0059.

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Fremes, Stephen E., Ren-Ke Li, Richard D. Weisel, Donald A. G. Mickle, and Laura C. Tumiati. "Prolonged hypothermic cardiac storage with University of Wisconsin solution." Journal of Thoracic and Cardiovascular Surgery 102, no. 5 (1991): 666–72. http://dx.doi.org/10.1016/s0022-5223(19)36855-2.

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Ku, Kwansong, Hidetaka Oku, Mohammed Shah Alam, Yuhei Saitoh, Seishi Nosaka, and Kengo Nakayama. "PROLONGED HYPOTHERMIC CARDIAC STORAGE WITH HISTIDINE-TRYPTOPHAN-KETOGLUTARATE SOLUTION." Transplantation 64, no. 7 (1997): 971–75. http://dx.doi.org/10.1097/00007890-199710150-00006.

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31

Holovina, Kseniia, Igor Kovalenko, and Olena Bobrova. "Influence of Oxidative Stress Erythrocyte State at Hypothermic Storage." Problems of Cryobiology and Cryomedicine 29, no. 2 (2019): 180. http://dx.doi.org/10.15407/cryo29.02.180.

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32

Bulwan, Maria, Magdalena Antosiak-Iwańska, Ewa Godlewska, Ludomira Granicka, Szczepan Zapotoczny, and Maria Nowakowska. "Chitosan-Based Nanocoatings for Hypothermic Storage of Living Cells." Macromolecular Bioscience 13, no. 11 (2013): 1610–20. http://dx.doi.org/10.1002/mabi.201300258.

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33

KITAMURA, MASAYA, OSAMU TACUSARI, TAKEHIDE AKIMOTO, et al. "In-storage Hypothermic Perfusion for Heart and Lung Transplantation." ASAIO Journal 38, no. 3 (1992): M163—M166. http://dx.doi.org/10.1097/00002480-199207000-00010.

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34

Coutts, Margaret, Steven Hinton, Jie Zheng, and David W. Scharp. "Hypothermic storage and preservation of human pancreatic acinar tissue." In Vitro Cellular & Developmental Biology - Animal 43, no. 1 (2007): 2–6. http://dx.doi.org/10.1007/s11626-006-9006-0.

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35

Nunes, Julien, Jason P. Acker, and Jelena L. Holovati. "117. Red blood cell adherence during 42day hypothermic storage." Cryobiology 63, no. 3 (2011): 338. http://dx.doi.org/10.1016/j.cryobiol.2011.09.120.

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Papas, Klearchos K. "21. Supplemented oxygenation during hypothermic storage: Friend or foe." Cryobiology 66, no. 3 (2013): 348. http://dx.doi.org/10.1016/j.cryobiol.2013.02.027.

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37

Tchir, Jayme D. R., Jason P. Acker, and Jelena L. Holovati. "Rejuvenation of ATP during storage does not reverse effects of the hypothermic storage lesion." Transfusion 53, no. 12 (2013): 3184–91. http://dx.doi.org/10.1111/trf.12194.

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Zaitsev, Alexander, Elizaveta Bragina, Mikhail Atroshchenko, et al. "PSV-39 The relationship between structural integrity and sperm survival during hypothermic storage of stallion sperm." Journal of Animal Science 98, Supplement_4 (2020): 335–36. http://dx.doi.org/10.1093/jas/skaa278.597.

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Abstract The aim of this work was to study the relationship between ultrastructural integrity and sperm survival during hypothermic storage of chilled and cryopreserved stallion sperm. Semen from 37 stallions, were collected during the breeding season. The structural integrity of sperm was studied using a Hitachi 700 electron microscope. Progressive motility was evaluated using an Olympus BX 41 phase contrast microscope. Sperm survival was defined as the ability of spermatozoa to maintain progressive motility above 5% during hypothermic (+4C) storage of sperm. Spearman nonparametric correlatio

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Fagbemi, Oluwole S., and Basil J. Northover. "Effect of Protein Kinase C Inhibitors and 2,3-Butanedione Monoxime on the Long-Term Hypothermic Preservation of Isolated Rat Hearts." Clinical Science 91, no. 6 (1996): 745–54. http://dx.doi.org/10.1042/cs0910745.

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1. This study examines the protective effect of staurosporine, chelerythrine, Ro 31-8220 and 2,3-butanedione monoxime in rat hearts during hypothermic storage. 2. Hearts were microperfused at 4°C for 24 or 48 h with a storage buffer that in some cases contained one of these protein kinase C inhibitors either alone or in combination with 2,3-butanedione monoxime. After hypothermic storage, hearts were rewarmed to 37°C with Krebs—Henseleit buffer. Cardiac function was then assessed in either Langendorff mode or working heart mode. 3. Compared with values from fresh non-stored hearts, hypothermic

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Huelsz-Prince, Guizela, Arthur L. DeVries, Huib J. Bakker, Jeroen S. van Zon, and Konrad Meister. "Effect of Antifreeze Glycoproteins on Organoid Survival during and after Hypothermic Storage." Biomolecules 9, no. 3 (2019): 110. http://dx.doi.org/10.3390/biom9030110.

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We study the effect of antifreeze glycoproteins (AFGPs) on the survival of organoids under hypothermic conditions. We find that the survival of organoids in cold conditions depends on their developmental stage. Mature organoids die within 24 h when being stored at 4 °C, while cystic organoids can survive up to 48 h. We find that in the presence of AFGPs, the organoid survival is prolonged up to 72 h, irrespective of their developmental stage. Fluorescence microscopy experiments reveal that the AFGPs predominately localize at the cell surface and cover the cell membranes. Our findings support a

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41

Ku, K., and H. Oku. "Cardiac function and myocardial energy level after prolonged hypothermic storage." Transplantation Proceedings 30, no. 7 (1998): 3331–33. http://dx.doi.org/10.1016/s0041-1345(98)01050-1.

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Tchir, Jayme D. R., Jason P. Acker, and Jelena L. Holovati. "60. Changes in red blood cell membrane during hypothermic storage." Cryobiology 63, no. 3 (2011): 322. http://dx.doi.org/10.1016/j.cryobiol.2011.09.063.

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43

Zhang, Ji, Robert D. Furukawa, and Stephen E. Fremes. "The Beneficial Effects of Heat-Shock for Prolonged Hypothermic Storage." Journal of Surgical Research 63, no. 1 (1996): 314–19. http://dx.doi.org/10.1006/jsre.1996.0267.

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Ideta, A., S. Tsuda, Y. Nishimiya, K. Tsuchiya, Y. Nakamura, and Y. Aoyagi. "46 HYPOTHERMIC STORAGE FOR 10 DAYS OF BOVINE EMBRYOS USING TYPE III ANTIFREEZE PROTEIN." Reproduction, Fertility and Development 26, no. 1 (2014): 137. http://dx.doi.org/10.1071/rdv26n1ab46.

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Recently, we developed a medium that enabled bovine embryos to be held for up to 7 days at 4°C (Tsuchiya et al. 2014 IETS meeting). To be of practical value, mammalian embryo preservation at hypothermic temperatures must be able to maintain viability for periods longer than 7 days. Antifreeze proteins (AFP) were discovered in various organisms (such as fish, insects, plants, and bacteria) living in cold regions. They show a unique ability to protect cold-sensitive cells from hypothermic damage. Here, we found that a biomolecule known as type III AFP solubilised into an optimized solvent can ke

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McANULTY, JONATHAN F. "Hypothermic Storage of Feline Kidneys for Transplantation: Successful Ex Vivo Storage Up to 7 Hours." Veterinary Surgery 27, no. 4 (1998): 312–20. http://dx.doi.org/10.1111/j.1532-950x.1998.tb00133.x.

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46

Bryan, C. L., A. J. Patefield, D. Cohen, J. L. Nielsen, B. Emanuel, and J. H. Calhoon. "Assessment of injury in transplanted and nontransplanted lungs after 6 h of cold storage with glutathione." Journal of Applied Physiology 76, no. 3 (1994): 1232–41. http://dx.doi.org/10.1152/jappl.1994.76.3.1232.

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Single-lung transplantation after 3 h of hypothermic storage produces bilateral lung injury [pulmonary reimplantation response (PRR)]. We hypothesized that glutathione (GSH) hypothermic storage would protect both lungs from PRR for extended preservation times and that differences in injury and protection would be realized between the graft and the nontransplanted lung. Mongrel dogs underwent left single-lung autotransplantation after preservation for 5–6 h in Euro-Collins (EC) solution, EC plus exogenous GSH (EC+GSH), or Viaspan (VIA) at 4 degrees C. Lung injury was measured in both lungs afte

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Mangino, Martin J., Mary Ametani, Csaba Szabó, and James H. Southard. "Poly(ADP-ribose) polymerase and renal hypothermic preservation injury." American Journal of Physiology-Renal Physiology 286, no. 5 (2004): F838—F847. http://dx.doi.org/10.1152/ajprenal.00230.2003.

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The nuclear enzyme poly(ADP-ribose) polymerase (PARP) has been implicated in ischemia-reperfusion injury in many tissues under normothermic conditions. The purpose of this study was to determine whether PARP contributes to mechanisms of the hypothermic ischemia-reperfusion injury that occurs when kidneys are cold stored for transplantation. Cortical tissue slice PARP enzyme activity rose significantly with prolonged cold storage and was dependent on both reperfusion and preservation quality. However, prior exposure to warm ischemia abrogated this increase. PARP protein increased with cold stor

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Hajjar, Gail, Luis H. Toledo-Pereyra, and Gerald H. MacKenzie. "Effects of 24-Hour Hypothermic Storage on Isolated-Perfused Canine Heart-Lungs." Perfusion 2, no. 2 (1987): 101–8. http://dx.doi.org/10.1177/026765918700200204.

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Isolated-perfused canine heart-lungs were used as a model to measure the effects of 24-hour hypothermic storage on cardiopulmonary function and metabolism. Heart-lungs were stored at 4–7°C in Euro-Collins solution ( n = 6) or TP-V ( n = 6). a hyperosmolar colloid solution containing dextrose, sucrose. ATP and MgCl2. Lung inflation was maintained with 100% nitrogen. Following preservation, the heart-lungs were perfused with an albumin-mannitol perfusate for three hours at 37°C. for functional and laboratory determinations. Cold storage with TP-V soiution resulted in significantly lower enzyme a

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Giannone, Ferdinando A., Davide Treré, Marco Domenicali, et al. "An Innovative Hyperbaric Hypothermic Machine Perfusion Protects the Liver from Experimental Preservation Injury." Scientific World Journal 2012 (2012): 1–9. http://dx.doi.org/10.1100/2012/573410.

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Purpose. Hypothermic machine perfusion systems seem more effective than the current static storage to prevent cold ischemic liver injury. Thus, we test an innovative hyperbaric hypothermic machine perfusion (HHMP), which combines hyperbaric oxygenation of the preservation solution and continuous perfusion of the graft.Methods. Rat livers were preserved with Celsior solution according to 4 different modalities:normobaric static preservation;hyperbaric static preservationat 2 atmosphere absolute (ATA);normobaric dynamic preservation, with continuous perfusion;hyperbaric dynamic preservation, wit

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Lagerstrom, Carl F., Derrick D. McElroy, Heinrich Taegtmeyer, and William E. Walker. "Improved recovery of cardiac function after hypothermic ischemic storage with ouabain." Journal of Thoracic and Cardiovascular Surgery 96, no. 5 (1988): 782–88. http://dx.doi.org/10.1016/s0022-5223(19)35188-8.

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