Journal articles: 'Idoso e a CPTI'

1

Carvalho, Daniel Rey de, Paulo Sérgio Perri de Carvalho, Osvaldo Magro Filho, José Daniel Biasoli de Mello, Márcio Mateus Beloti, and Adalberto Luiz Rosa. "Characterization and in vitro cytocompatibility of an acid-etched titanium surface." Brazilian Dental Journal 21, no. 1 (January 2010): 3–11. http://dx.doi.org/10.1590/s0103-64402010000100001.

Full text

Abstract:

The aims of this study were to characterize the microstructure of a commercially pure titanium (cpTi) surface etched with HCl/H2SO4 (AE-cpTi) and to investigate its in vitro cytocompatibility compared to turned cpTi (T-cpTi). T-cpTi showed a grooved surface and AE-cpTi revealed a surface characterized by the presence of micropits. Surface parameters indicated that the AE-cpTi surface is more isotropic and present a greater area compared to T-cpTi. The oxide film thickness was similar between both surfaces; however, AE-cpTi presented more Ti and O and less C. Osteoblastic cell proliferation, alkaline phosphatase activity, and bone-like nodule formation were greater on T-cpTi than on AE-cpTi. These results show that acid etching treatment produced a surface with different topographical and chemical features compared to the turned one, and such surface modification affected negatively the in vitro cytocompatibility of cpTi as demonstrated by decreasing culture growth and expression of osteoblastic phenotype.

APA, Harvard, Vancouver, ISO, and other styles

2

Colins, Olivier F., Violaine Veen, Margo Veenstra, Louise Frogner, and Henrik Andershed. "The Child Problematic Traits Inventory in a Dutch General Population Sample of 3- to 7-Year-Old Children." European Journal of Psychological Assessment 34, no. 5 (September 2018): 336–43. http://dx.doi.org/10.1027/1015-5759/a000347.

Full text

Abstract:

Abstract. The Child Problematic Traits Inventory (CPTI) is a new teacher rated instrument to assess psychopathic personality traits in children. This is the first study to test the psychometric properties of the Dutch CPTI. Teachers completed the CPTI for 287 3- to 7-year old-children. Results from confirmatory factor analysis supported the proposed 3-factor structure of the CPTI. The CPTI total score and three factor scores were internally consistent and showed the expected correlations with external criterion measures that have been linked to psychopathic personality, including conduct problems, proactive and reactive aggression, and temperament. This study also provides novel evidence that CPTI factor scores were positively related to an alternative measure of callous-unemotional traits, thereby supporting the criterion validity of the CPTI scores. In conclusion, this study replicates and extends prior tests of the CPTI in Sweden and suggests that the Dutch version of the CPTI holds promise as a teacher rated tool for assessing psychopathic traits in childhood.

APA, Harvard, Vancouver, ISO, and other styles

3

Colins, Olivier F., Peter J. Roetman, Laura Lopez-Romero, and Henrik Andershed. "Assessing Psychopathic Traits Among Children: The First Validation Study of the Child Problematic Traits Inventory in a Clinical Sample." Assessment 27, no. 6 (March 7, 2019): 1242–57. http://dx.doi.org/10.1177/1073191119832654.

Full text

Abstract:

This is the first study that tested the psychometric properties of the Child Problematic Traits Inventory (CPTI) in clinic-referred children (ages 6-13 years). Teachers ( N = 159) and parents ( N = 173) completed the CPTI and various other measures. Confirmatory factor analyses supported the CPTI’s three-factor structure when teachers and parents rated the 28 CPTI items. Teacher- and parent-reported CPTI scores showed the expected relations with external correlates (e.g., conduct problems and proactive aggression). Crucially, the validity of the CPTI scores was also supported across informants (i.e., when linking teacher-reported CPTI scores to parent-reported external correlates, and vice versa) and across methods (i.e., regardless if a questionnaire or a diagnostic interview was used to measure external correlates). We conclude that the CPTI holds promise as a research tool for assessing psychopathic traits in clinic-referred children. Until our findings have been replicated and extended, the CPTI should not be used for clinical decision making.

APA, Harvard, Vancouver, ISO, and other styles

4

Chen, Z. H., L. J. Chen, Y. L. Zhang, and Z. J. Wu. "Microbial properties, enzyme activities and the persistence of exogenous proteins in soil under consecutive cultivation of transgenic cottons (Gossypium hirsutum L.)." Plant, Soil and Environment 57, No. 2 (February 17, 2011): 67–74. http://dx.doi.org/10.17221/237/2010-pse.

Full text

Abstract:

One Bacillus thuringiensis (Bt) and two stacked Bt and cowpea trypsin inhibitor (Bt + CpTI) cottons and their non-transgenic isolines were consecutively cultivated to investigate the soil persistence of Cry1Ac and CpTI proteins and their effects on microbial properties and enzyme activities involving C, N, P, and S cycling in soil. Results showed that there were the persistence of Cry1Ac and CpTI proteins in soil under 4-year consecutive cultivation of transgenic cottons. Cry1Ac proteins varied from 6.75 ng/g to 12.01 ng/g and CpTI proteins varied from 30.65 to 43.60 ng/g. However, neither of these two proteins was detected in soil under non-transgenic cottons. Soil microbial biomass carbon, microbial activities, and soil enzyme activities (except urease and phosphodiesterase) significantly decreased in soil under transgenic cottons. Correlation analysis showed that most of microbial properties and enzyme activities in soil had a negative relationship with Cry1Ac content, while most of them had a positive relationship with CpTI content. Our data indicate that consecutive cultivation by genetically modified cottons with Bt and CpTI genes can result in persistence of Cry1Ac and CpTI proteins and negatively affect soil microbial and biochemical properties.

APA, Harvard, Vancouver, ISO, and other styles

5

Nguyen, Linh Thi My, Thanh Nguyen Chu, and Le Van Bui. "A real-time PCR method for detection of CpTI (Cowpea Trypsin Inhibitor) gene in the genetically modified rice originating from China." Science and Technology Development Journal 18, no. 2 (June 30, 2015): 74–86. http://dx.doi.org/10.32508/stdj.v18i2.1145.

Full text

Abstract:

Labelling and traceability of genetically modified organisms (GMOs) are necessary for trade and regulation in the world and Vietnam. Cowpea Trypsin Inhibitor (CpTI) gene encodes a trypsin inhibitor which is considered as a suitable candidate for developing insect-resistant transgenic plants, especially transgenic rice lines originating from China. In this study, we established a real-time PCR protocol to detect the CpTI gene in transgenic rice. The protocol with CpTI-F and CpTI-R primers, 300 nM primers, 0.5 X SYBR Green I, annealing temperature at 62 0C showed the best results. Amplification efficiency is 94.64 % and the limit of detection is 50 copies. Moreover, PCR product of CpTI gene was cloned into pBluescript plasmid using as a positive control

APA, Harvard, Vancouver, ISO, and other styles

6

López-Romero, Laura, Beatriz Molinuevo, Albert Bonillo, Henrik Andershed, Olivier F. Colins, Rafael Torrubia, and Estrella Romero. "Psychometric Properties of the Spanish Version of the Child Problematic Traits Inventory in 3- to 12-Year-Old Spanish Children." European Journal of Psychological Assessment 35, no. 6 (November 2019): 842–54. http://dx.doi.org/10.1027/1015-5759/a000458.

Full text

Abstract:

Abstract. The aim of this study was to test the reliability and validity of the Spanish teacher-rated Child Problematic Traits Inventory (CPTI) in two community samples of 3- to 12-year-old children. Confirmatory Factor Analysis (CFA) supported the three-factor structure of the CPTI (Grandiose-Deceitful: GD; Callous-Unemotional: CU; Impulsive-Need for Stimulation: INS), being invariant across gender and age groups. The CPTI total and factor scores showed excellent internal consistencies (> .90) in the total group, and across gender and age groups. In support of their criterion validity, the CPTI scores were positively related to psychopathy scores as measured by an alternative teacher-rated measure. In support of their convergent validity, the CPTI scores showed the expected relations to variables that have been linked to psychopathic personality, including fearlessness, conduct problems, aggression, and low prosocial behavior. Overall, these findings suggest that the Spanish teacher-rated version of the CPTI has good psychometric properties and seems to be a promising tool for studying psychopathic traits in children.

APA, Harvard, Vancouver, ISO, and other styles

7

Shehbaz, Tauheed, Fahd Nawaz Khan, Massab Junaid, and Julfikar Haider. "Investigating Nanoindentation Creep Behavior of Pulsed-TIG Welded Inconel 718 and Commercially Pure Titanium Using a Vanadium Interlayer." Metals 11, no. 9 (September 20, 2021): 1492. http://dx.doi.org/10.3390/met11091492.

Full text

Abstract:

In a dissimilar welded joint between Ni base alloys and titanium, creep failure is a potential concern as it could threaten to undermine the integrity of the joint. In this research, the mechanical heterogeneity of a Pulsed TIG welded joint between commercially pure titanium (CpTi) and Inconel 718 (IN718) with a vanadium (V) interlayer was studied through a nanoindentation technique with respect to hardness, elastic modulus, and ambient temperature creep deformation across all regions (fusion zones and interfaces, mainly composed of a dendritic morphology). According to the experimental results, a nanohardness of approximately 10 GPa was observed at the V/IN718 interface, which was almost 70% higher than that at the V/CpTi interface. This happened due to the formation of intermetallic compounds (IMCs) (e.g., Ti2Ni, NiV3, NiTi) and a (Ti, V) solid solution at the V/IN718 and V/CpTi interfaces, respectively. In addition, nanohardness at the V/IN718 interface was inhomogeneous as compared to that at the V/CpTi interface. Creep deformation behavior at the IN718 side was relatively higher than that at different regions on the CpTi side. The decreased plastic deformation or creep effect of the IMCs could be attributed to their higher hardness value. Compared to the base metals (CpTi and IN718), the IMCs exhibited a strain hardening effect. The calculated values of the creep stress exponent were found in the range of 1.51–3.52 and 2.52–4.15 in the V/CpTi and V/IN718 interfaces, respectively. Furthermore, the results indicated that the creep mechanism could have been due to diffusional creep and dislocation climb.

APA, Harvard, Vancouver, ISO, and other styles

8

Murthy, M. S. R., and S. V. Pande. "Characterization of a solubilized malonyl-CoA-sensitive carnitine palmitoyltransferase from the mitochondrial outer membrane as a protein distinct from the malonyl-CoA-insensitive carnitine palmitoyltransferase of the inner membrane." Biochemical Journal 268, no. 3 (June 15, 1990): 599–604. http://dx.doi.org/10.1042/bj2680599.

Full text

Abstract:

By using octyl glucoside in the presence of glycerol, it is possible to obtain a solubilized malonyl-CoA-sensitive carnitine palmitoyltransferase (CPTo) from the outer membranes of rat liver mitochondria. H.p.l.c. on hydroxyapatite column has now allowed a clear separation of the CPTo from the malonyl-CoA-insensitive CPT activity of the inner membranes (CPTi). The separated CPTo activity showed inhibition by low micromolar concentrations of malonyl-CoA, 2-tetradecylglycidyl-CoA and etomoxir-CoA. On solubilization and fractionation, the CPTo rapidly lost activity, unlike the relatively stable CPTi activity. Reconstitution into asolectin liposomes enhanced the activity and the malonyl-CoA-sensitivity of the CPTo fractions, whereas it had no such effect on the activity or malonyl-CoA insensitivity of the CPTi fractions. A polyclonal antibody raised against the malonyl-CoA-insensitive enzyme, purified from the inner membranes, precipitated the CPTi activity, but showed no reactivity with the CPTo fractions. In Western blots, the above antibody did not react with any polypeptide of the CPTo fractions. Incubation of the outer-membrane preparations with [3H]etomoxir, in the presence of ATP and CoA, led to labelling of a 90 kDa polypeptide that in the above hydroxyapatite chromatography was eluted in the same region as the CPTo. No such polypeptide labelling was seen in the CPTi fractions. With heart and skeletal-muscle mitochondria, the correspondingly labelled polypeptide was of about 86 kDa. These results show that the CPTo and CPTi are distinct proteins, that a subunit of 90 kDa for liver and 86 kDa for muscle constitutes a component of their respective CPTo systems, and that the 66 kDa subunit of the CPTi does not constitute a part of the CPTo system.

APA, Harvard, Vancouver, ISO, and other styles

9

Zhang, Q., Z. Y. Zhang, S. Z. Lin, and Y. Z. Lin. "Resistance of Transgenic Hybrid Triploids in Populus tomentosa Carr. Against 3 Species of Lepidopterans Following Two Winter Dormancies Conferred by High Level Expression of Cowpea Trypsin Inhibitor Gene." Silvae Genetica 54, no. 1-6 (December 1, 2005): 108–16. http://dx.doi.org/10.1515/sg-2005-0017.

Full text

Abstract:

Abstract Hybrid triploid poplars [(P. tomentosa x P. bolleana) x P. tomentosa] genetically engineered with cowpea trypsin inhibitor (CpTI) gene have been out-planted in field for two years. They were used to detect their efficacy against 3 species of poplar defoliators: forest tent capterpillar, Malacosoma disstria Hübner, gypsy moth, Lymantria dispar Linnaeus and willow moth, Stilpnotia candida Staudinger by using detached leaves and for the purpose of identifying the CpTI gene at the molecular level. Foliage of transgenic poplars elicited an increase in larval mortality rate and a decrease in foliage consumption, wet weight gains, faeces excretion, deposited pupae number and pupae weight, thus indicating its effectiveness in affecting the growth, development and fecundity of larvae rather than only directly killing them. PCR and Southern blotting analyses confirmed the stable incorporation of CpTI gene while proteinase inhibitory assays disclosed its high level expression in the two-field-season of transgenic trees. Efficacious insect resistance and higher content of CpTI in foliage were found in transgenic clone TG04, TG07, TG08 and TG71, demonstrating a correspondence between the insect resistance level and the CpTI content in the foliage of transgenic poplar.

APA, Harvard, Vancouver, ISO, and other styles

10

Colins, Olivier F., Kostas Fanti, Henrik Larsson, and Henrik Andershed. "Psychopathic Traits in Early Childhood: Further Validation of the Child Problematic Traits Inventory." Assessment 24, no. 5 (January 4, 2016): 602–14. http://dx.doi.org/10.1177/1073191115624544.

Full text

Abstract:

The aim was to further test the reliability and validity of a newly developed instrument designed to assess psychopathic personality traits in children, the Child Problematic Traits Inventory (CPTI). Data from the Preschool Twin Study in Sweden were used, a national general population study of 5-year-old twins ( n = 1,188, 50.3% girls). Both preschool teachers and parents were used as informants. Confirmatory factor analysis replicated the intended three-factorial structure of the 28 items of the CPTI. Overall, our findings demonstrated good internal consistency and convergent validity, with all the teacher-rated CPTI scores being associated with teacher and parent ratings of externalizing psychopathology, aggressive behavior, fearlessness, and prosocial peer involvement. In conclusion, the CPTI hold promise as a teacher-rated tool for assessing psychopathic traits in childhood, though more research is needed to see if these findings can be generalized to other countries, settings, and older children.

APA, Harvard, Vancouver, ISO, and other styles

11

Sabino, Tatiana de Andrade, Laís Regiane da Silva-Concílio, Ana Christina Elias Claro Neves, Ana Paula Rosifini Alves Claro, Marina Amaral, Rafael Pino Vitti, and Cristiane Aparecida de Assis Claro. "Stress distribution in the peri-implant area of pure titanium and titanium-zirconium small implants." Brazilian Journal of Oral Sciences 18 (November 12, 2019): e191505. http://dx.doi.org/10.20396/bjos.v18i0.8657252.

Full text

Abstract:

Aim: In dental implant treatment, there is a demand for mechanically stronger implants. Despite the existence of several studies showing the clinical success of narrow diameter implants, most of them are based on pure titanium (cpTi) alloys. There is a few clinical evidences of the success rate of titanium-zirconium (TiZr) narrow diameter implants. The aim of this study was to evaluate the stress distribution in the peri-implant area of narrow diameter cpTi and TiZr implants under axial and oblique loads. Methods: Photoelastic models were produced using epoxy resin (PL2, Vishay Precision Group) from a master model. The implants (cpTi and TiZr; Straumann AG) had 3.3 mm in diameter and 12 mm in height. Loads of 100 N and 200 N were applied to the abutment at angles of 0° (axial), 10°, 20°, and 30° (oblique). A circular polariscope (Eikonal) was used under dark field white-light configuration. The isochromatic fringes were analyzed in the peri-implant region in 5 areas, using ASTM table with isochromatic fringes; cervical-mesial, cervical-distal, mid-mesial, mid-distal and apical. Results: In general, under axial and oblique loads, the stress in the TiZr implant was lower than in the cpTi implant. The load of 200 N produced the highest stress values in cpTi and TiZr implants. In both implants and loads, the fringes were located more in apical area at all angles evaluated. Conclusion: It can be concluded that for small implants, the load inclination and intensity change the pattern of stress distribution and the cpTi implant exhibited the highest peri-implant stress.

APA, Harvard, Vancouver, ISO, and other styles

12

Łosiewicz, Bożena, Joanna Maszybrocka, Julian Kubisztal, Grzegorz Skrabalak, and Andrzej Stwora. "Corrosion Resistance of the CpTi G2 Cellular Lattice with TPMS Architecture for Gas Diffusion Electrodes." Materials 14, no. 1 (December 26, 2020): 81. http://dx.doi.org/10.3390/ma14010081.

Full text

Abstract:

The corrosion of materials used in the design of metal-air batteries may shorten their cycle life. Therefore, metal-based materials with enhanced electrochemical stability have attracted much attention. The purpose of this work was to determine the corrosion resistance of commercially pure titanium Grade 2 (CpTi G2) cellular lattice with the triply periodic minimal surfaces (TPMS) architecture of G80, D80, I-2Y80 in 0.1 M KOH solution saturated with oxygen at 25 °C. To produce CpTi G2 cellular lattices, selective laser melting technology was used which allowed us to obtain 3D cellular lattice structures with a controlled total porosity of 80%. For comparison, the bulk electrode was also investigated. SEM examination and statistical analysis of the surface topography maps of the CpTi G2 cellular lattices with the TPMS architecture revealed much more complex surface morphology compared to the bulk CpTi SLM. Corrosion resistance tests of the obtained materials were conducted using open circuit potential method, Tafel curves, anodic polarization curves, and electrochemical impedance spectroscopy. The highest corrosion resistance and the lowest material consumption per year were revealed for the CpTi G2 cellular lattice with TPMS architecture of G80, which can be proposed as promising material with increased corrosion resistance for gas diffusion in alkaline metal-air batteries.

APA, Harvard, Vancouver, ISO, and other styles

13

Klapötke, Thomas, Regina Laskowski, and Hartmut Köpf. "Synthese und spektroskopische Charakterisierung neuer Mono(cyclopentadienyl)titan(IV)thiolat-Komplexe des Typs CpTi(SR)n(Cl)3-n mit n = 1, 2, 3 / Synthesis and Spectroscopic Characterization of New Mono(cyclopentadienyl)titanium(IV) Thiolate Complexes of the Type CpTi(SR)n(Cl)3-n n =1,2,3." Zeitschrift für Naturforschung B 42, no. 6 (June 1, 1987): 777–80. http://dx.doi.org/10.1515/znb-1987-0623.

Full text

Abstract:

Abstract The mono(cyclopentadienyl)titan(IV)thiolat-Komplexe des Typs CpTi(SR)"(Cl) 3 _ n mit n = 1, 2, 3 Synthesis and Spectroscopic Characterization of New Mono(cyclopentadienyl)titanium(IV) Thiolate Complexes of the Type CpTi(SR)"(Cl) 3 _", n =1,2,3 Cyclopentadienyl-dichloro-thiolato-titanium(IV) Complexes, Cyclopentadienyl-chloro-bis(thiolato)-titanium(IV) Complexes, Cyclopentadienyl-tris(thiolato)-titanium(IV) Complexes The mono(cyclopentadienyl)titanium(IV) derivatives CpTi(SR)Cl-> (Cp = ?7 5 -C 5 H 5 ; R = QF S , C 6 H 4 Cl-p, C 6 H 4 Br-p, C6H4CH3-O, C 6 H 4 CH 3 -p, C 6 H", C,H,)\ Cp'Ti(SR)Cl 2 (Cp' = >7 5 -C 5 H 4 CH 3 ; R = C 6 H 5 , C 6 H 4 C1 -p, C 6 H 4 CH 3 -o, C 6 H 4 CH 3 -/>), CpTi(SR) 2 Cl (R = C 6 H 5), Cp'Ti(l,2-S 2 C 6 H 3 CH 3 -4)Cl, and CpTi(SR) 3 (R = C 6 H 5) were prepared by reaction of CpTiCl 3 or Cp'TiCl 3 with equivalent amounts of the appropriate lithium thiolates, or with the free thiol and triethylamine, respectively. The new thiolate complexes were characterized by 'H NMR and mass spectroscopy.

APA, Harvard, Vancouver, ISO, and other styles

14

Al Wazzan, Khalid A., and Ahmad A. Al-Nazzawi. "Marginal and Internal Adaptation of Commercially Pure Titanium and Titanium- Aluminum-Vanadium Alloy Cast Restorations." Journal of Contemporary Dental Practice 8, no. 1 (January 2007): 19–26. http://dx.doi.org/10.5005/jcdp-8-1-19.

Full text

Abstract:

Abstract Aim The purpose of this in vitro study was to investigate the marginal accuracy and internal fit of complete cast crowns and three-unit fixed partial dentures (FPDs) cast with commercially pure titanium (CPTi) and Titanium-Aluminum-Vanadium alloy (Ti-6Al-4V). Methods and Materials CPTi and Ti-6Al-4V alloy were used to cast twelve single crowns and twelve three-unit FPDs. A traveling microscope was used to measure marginal gap and discrepancies in internal fit. Two and oneway analysis of variance (ANOVA) analyses were used to determine the effects of the marginal and internal fit discrepancies. Results The Ti-6Al-4V alloy demonstrated a significantly smaller marginal gap than CPTi (P<0.0001). The recorded marginal discrepancies for both metals were within a clinically accepted range (<100 ìm). The single crown fit discrepancy was significantly smaller than the three-unit FPD for both the CPTi and the Ti-6Al-4V alloy (P<0.0001). For the internal fit discrepancy, the occlusal surface showed the greatest gaps. Conclusions The Ti-6Al-4V alloy demonstrated a better fit than CPTi. Single crowns showed an improved fit when compared with the three-unit FPD. Mid-occlusal internal gap demonstrated greater values than the axial internal gap. Clinical Implications This in vitro study suggested marginal fit of complete crowns and three-unit FPDs cast by CPTi or Ti-6Al-4V alloy were within the range of what is clinically acceptable for longevity of restorations. Citation Al Wazzan KA, Al-Nazzawi AA. Marginal and Internal Adaptation of Commercially Pure Titanium and Titanium-Aluminum-Vanadium Alloy Cast Restorations. J Contemp Dent Pract 2007 January;(8)1:019-026.

APA, Harvard, Vancouver, ISO, and other styles

15

Tanabe, H., Takehiko Watanabe, Y. Abe, and A. Yanagisawa. "Solid State Welding between CPTi and AZ31B Magnesium Alloy by Friction Stirring." Materials Science Forum 539-543 (March 2007): 3856–62. http://dx.doi.org/10.4028/www.scientific.net/msf.539-543.3856.

Full text

Abstract:

This paper described an experimental study on the friction stir welding of dissimilar metal between commercially pure titanium (CPTi) and magnesium alloy AZ31B (Mg). Butt-welds produced at various tool rotation speeds were evaluated by the observation of the weld surface appearance, X-ray radiographic test, tensile test, SEM and EDX analysis. The main results obtained are as follows. Butt-joint welding of the CPTi plate to the Mg plate was easily and successfully achieved. The ignition of Mg occurred during welding at the tool rotation speeds over 1200rpm. The fragments of CPTi existed in a continuous form in Mg matrix. The tool rotation speed of 1200rpm attained the maximum tensile strength of a joint, which was about 75% of that of Mg base metal. Fracture occurred at the weld interface in most joint. EDX analysis revealed that Al in the Mg diffused into CPTi through the weld interface and MgO existed at the interface. It seems that the decrease in Al concentration in the Mg and MgO formation around the weld interface caused the low tensile strength of the joints.

APA, Harvard, Vancouver, ISO, and other styles

16

Goia, Tamiye Simone, Kalan Bastos Violin, José Carlos Bressiani, and Ana Helena de Almeida Bressiani. "Titanium and Ti-13Nb-13Zr Alloy Porous Implants Obtained by Space-Holder Technique with Addition of Albumin." Key Engineering Materials 529-530 (November 2012): 574–79. http://dx.doi.org/10.4028/www.scientific.net/kem.529-530.574.

Full text

Abstract:

Titanium and its alloys are the main metals studied as porous metallic implants by their excellent mechanical properties and biological interactions. Production methods of porous metallic materials are based on powder metallurgy (PM), because it allows the manufacturing of parts with complex shapes and dimensions close to the finals (near-net shape), and the addition of alloying elements reaching a satisfactory structural homogeneity, and porosity. The pore production by space-holder technique constitutes of mixing organic compounds with metal powder, which when removed by thermal treatment prior structures are kept in place. The objective of this study is to obtain porous implants of commercially pure titanium (cpTi) and Ti-13Nb-13Zr alloy by PM with space-holder technique and albumin as an additive. For the processing of the samples were used hydride titanium powder (TiH2) to obtain cpTi samples, and metal powders of Ti, Nb and Zr in the stoichiometric proportions for obtaining the alloy samples. The samples were prepared by mixing the metallic powder to the albumin (30wt%) and filling a silicone model that was pressed isostatically (140 MPa). The thermal treatment was performed in an oxidizing atmosphere (350°C/1h) for the decomposition of organic material. The sintering was performed at a temperature of 1300°C (1h/cpTi, 3h/Alloy) in high vacuum furnace (10-5 mBar) to all samples. The calculated porosity showed a significant difference between the samples cpTi (40%) and alloy (60%). The samples surface characterization showed very rough with high specific surface area. Samples of cpTi presented formation of necks arising from sintering. In the alloy samples were observed homogenous microstructure with the presence of α and β phases composing the Widmanstätten structure. It is possible to conclude that the same amount albumin allowed the formation of pores in the microstructure of cpTi and alloy although in different proportions, without harming the sintering of both and allowing diffusion of the alloy elements.

APA, Harvard, Vancouver, ISO, and other styles

17

Holloway, Graham Paul. "The role of protein-mediated transport in regulating mitochondrial long-chain fatty acid oxidation." Applied Physiology, Nutrition, and Metabolism 33, no. 1 (February 2008): 141–42. http://dx.doi.org/10.1139/h07-172.

Full text

Abstract:

This thesis is an investigation of the role of fatty acid translocase (FAT/CD36), plasma membrane associated fatty acid binding protein (FABPpm), and carnitine palmitoyltransferase I (CPTI) in transporting long-chain fatty acids (LCFAs) across mitochondrial membranes. Maximal CPTI activity, as well as the sensitivity of CPTI for its substrate palmitoyl-CoA (P-CoA) and its inhibitor malonyl-CoA (M-CoA), were measured in mitochondria isolated from human vastus lateralis muscles at rest and following muscle contraction. Exercise did not alter maximal CPTI activity or the sensitivity of CPTI for P-CoA. In contrast, exercise progressively attenuated the ability of M-CoA to inhibit CPTI activity. Mitochondrial FAT/CD36 protein content was also measured at rest, during, and following 2 h of cycling at ~60% maximal oxygen uptake. Exercise progressively increased the content of mitochondrial FAT/CD36 (+59%), which was significantly (p < 0.05) correlated with palmitate oxidation during exercise (r = 0.52), while palmitate oxidation was inhibited ~80% by the administration of a specific FAT/CD36 inhibitor. These data suggest that alterations in CPTI M-CoA sensitivity and increases in mitochondrial FAT/CD36 coordinate exercise-induced increases in fatty acid oxidation. FABPpm, another plasma membrane transport protein, has identical amino acid sequence to mitochondrial aspartate aminotransferase (mAspAT). Since FABPpm contributes to plasma membrane fatty acid transport, the role of FABPpm with respect to mitochondrial LCFA transport was investigated. However, unlike FAT/CD36, muscle contraction did not induce an increase in mitochondrial FABPpm protein in rat or human skeletal muscle. In addition, electrotransfecting FABPpm cDNA into rat skeletal muscle upregulated this protein in mitochondria by 80% without altering mitochondrial palmitate oxidation. In contrast, electrotransfection increased mAspAT activity by 90%, and this was correlated (r = 0.75; p < 0.01) with FABPpm protein. These data suggest that FABPpm does not contribute to the regulation of mitochondrial LCFA transport. Previously, it has been suggested that mitochondria from obese individuals contain an inherent dysfunction to oxidize LCFAs. In age-matched lean (BMI = 23.3 ± 0.7 kg·m–2) and obese (BMI = 37.6 ± 2.2 kg·m–2) individuals, isolated mitochondrial palmitate oxidation was not altered. In addition, mitochondrial FAT/CD36 content was not different in lean and obese individuals. In contrast, citrate synthase and β-hydroxyacyl-CoA dehydrogenase, common markers of total mitochondrial content, were decreased with obesity. Therefore, the decrease in mitochondrial content appeared to account for the observed reductions in whole-muscle LCFA oxidation.

APA, Harvard, Vancouver, ISO, and other styles

18

Pandya, Vamangi M., and Sachin A. Joshi. "FABRICATION OF NOVEL ANTICANCER POLYOXOMETALATE [CoW11O39(CpTi)]7- -CHITOSAN NANO-COMPOSITE, ITS TOXICITY REDUCTION AND SUSTAINED RELEASE." Asian Journal of Pharmaceutical and Clinical Research 10, no. 4 (April 1, 2017): 278. http://dx.doi.org/10.22159/ajpcr.2017.v10i4.16721.

Full text

Abstract:

Objectives: Polyoxometalates (POMs) are proved to be important for applications in medicine and in material science. Here, we representnanocomposite formation of tungsten-containing potent anticancer polyanion, K 6 H [CoW 11 O 39 (CpTi)].13H 2 O (CoW CpTi) with biocompatible ChitosanYC-100 (CSYC100) with the goal to reduce its heavy metal toxicity.Methods: Synthesis of “POM-CSYC100 nanocomposite” was attained without the aid of any cross-linker through electrostatic interaction technique. Nanocomposites were characterized using Fourier transform infrared spectroscopy, dynamic light scattering, transmission electron microscopy, and thermogravimetric analysis. The release profile recorded was slow and sustained at physiological pH. In vitro cytotoxicity assays which show an attribute to reduce the toxicity of these POM were performed on C2C12 (mouse myoblast cell line) and A-549 (lung cancer cell line), which proved the reduced toxicity of nanocomposites as compared to the bare drugs.Results: Sustained release studies showed there was a slow and steady release of CoW CpTi for 11 hrs, with the 98% of collective release at the end. From in vitro cytotoxic assay, it was deduced that CoW 11 11 CpTi -CSYC100 nanocomposite at the concentrations of 1.25 mM, and lower did not exhibit toxic effect on C2C12 cells as 95% total C2C12 cell mass remained viable. While in the case of A549 cells highest 5 mM concentration of bare CoW 11 CpTi is toxic to the cancer cells and after encapsulation cell viability increases from 10% to 55%.Conclusion: Thus, this study has designated the probability of using POM-chitosan nanocomposite for less toxic and effective biomedicinal applications.Keywords: Anticancer, Chitosan, Nanocomposite, In vitro cytotoxicity, Drug release.

APA, Harvard, Vancouver, ISO, and other styles

19

Osak, Patrycja, Joanna Maszybrocka, Julian Kubisztal, Patryk Ratajczak, and Bożena Łosiewicz. "Long-Term Assessment of the In Vitro Corrosion Resistance of Biomimetic ACP Coatings Electrodeposited from an Acetate Bath." Journal of Functional Biomaterials 12, no. 1 (February 7, 2021): 12. http://dx.doi.org/10.3390/jfb12010012.

Full text

Abstract:

Calcium phosphate coatings are able to improve the osseointegration process due to their chemical composition, which is similar to that of bone tissues. In this work, to increase the long-term corrosion resistance and to improve the osseointegration process of commercially pure titanium Grade 4 (CpTi G4), biomimetic amorphous calcium phosphate (ACP) coatings were electrodeposited for the first time from an acetate bath with a pH level of 7.0 and a Ca:P ratio of 1.67. ACP coatings were obtained on CpTi G4 substrate subjected to sandblasting and autoclaving using electrochemically assisted deposition at a potential of −3 V relative to the open circuit potential for 30 min at room temperature. SEM, EDS, 2D roughness profiles, amplitude-sensitive eddy current method, and Kelvin scanning probe were used for the surface characterization of the biomaterial under study. In vitro corrosion resistance tests were conducted for 21 days in artificial saliva using open circuit potential, polarization curves, and electrochemical impedance spectroscopy measurements. The passive-transpassive behavior was revealed for the obtained ACP coatings. The long-term corrosion resistance test showed a deterioration of the protective properties for CpTi G4 uncoated and coated with ACP with immersion time. The mechanism and kinetics of the pitting corrosion on the CpTi G4|TiO2|ACP coating system are discussed in detail.

APA, Harvard, Vancouver, ISO, and other styles

20

Murthy, M. S., and S. V. Pande. "Some differences in the properties of carnitine palmitoyltransferase activities of the mitochondrial outer and inner membranes." Biochemical Journal 248, no. 3 (December 15, 1987): 727–33. http://dx.doi.org/10.1042/bj2480727.

Full text

Abstract:

Recent evidence has shown that the outer, overt, malonyl-CoA-inhibitable carnitine palmitoyltransferase (CPTo) activity resides in the mitochondrial outer membrane [Murthy & Pande (1987) Proc. Natl. Acad. Sci. U.S.A. 84, 378-382]. A comparison of CPTo activity of rat liver mitochondria with the inner, initially latent, carnitine palmitoyltransferase (CPTi) of the mitochondrial inner membrane has revealed that the presence of digitonin and several other detergents inactivates CPTo activity. The CPTi activity, in contrast, was markedly stimulated by various detergents and phospholipid liposomes. These findings explain why in previous studies, which used digitonin or other detergents to expose, separate and purify the CPT activities, the inferences were drawn that (a) the ratio of latent to overt CPT was quite high, (b) both the CPT activities could be ascribed to one active protein recovered, and (c) the observed lack of malonyl-CoA inhibition indicated possible loss/separation of a putative malonyl-CoA-inhibition-conferring protein. Although both CPTo and CPTi were found to catalyse the forward and the backward reactions, CPTo showed greater capacity for the forward reaction and CPTi for the backward reaction. The easily solubilizable CPT, released on sonication of mitoplasts or of intact mitochondria under hypo-osmotic conditions, resembled CPTi in its properties. When octyl glucoside was used under appropriate conditions, 40-50% of the CPTo of outer membranes became solubilized, but it showed limited stability and decreased malonyl-CoA sensitivity. Malonyl-CoA-inhibitability of CPTo was decreased also on exposure of outer membranes to phospholipase C. When outer membranes that had been exposed to octyl glucoside or to phospholipase C were subjected to a reconstitution procedure using asolectin liposomes, the malonyl-CoA-inhibitability of CPTo was restored. A role of phospholipids in the malonyl-CoA sensitivity of CPTo is thus indicated.

APA, Harvard, Vancouver, ISO, and other styles

21

Faria, Adriana Cláudia Lapria, Rodrigo Lino de Matos, Renata Cristina Silveira Rodrigues, Rossana Pereira de Almeida Antunes, Ricardo Faria Ribeiro, and Maria da Gloria Chiarello de Mattos. "Comparative study of chemical and mechanical retentive systems for bonding of indirect composite resin to commercially pure titanium." Brazilian Dental Journal 19, no. 2 (2008): 134–38. http://dx.doi.org/10.1590/s0103-64402008000200009.

Full text

Abstract:

This study evaluated the effect of chemical and mechanical surface treatments for cast metal alloys on the bond strength of an indirect composite resin (Artglass) to commercially pure titanium (cpTi). Thirty cylindrical metal rods (3 mm diameter x 60 mm long) were cast in grade-1 cpTi and randomly assigned to 6 groups (n=5) according to the received surface treatment: sandblasting; chemical treatment; mechanical treatment - 0.4 mm beads; mechanical treatment - 0.6 mm beads; chemical/mechanical treatment - 0.4 mm; and chemical/mechanical treatment - 0.6 mm beads. Artglass rings (6.0 mm diameter x 2.0 mm thick) were light cured around the cpTi rods, according manufacturer's specifications. The specimens were invested in hard gypsum and their bond strength (in MPa) to the rods was measured at fracture with a universal testing machine at a crosshead speed of 2.0 mm/min and 500 kgf load cell. Data were analyzed statistically by one-way ANOVA and Tukey test (a=5%). The surface treatments differed significantly from each other (p<0.05) regarding the recorded bond strengths. Chemical retention and sandblasting showed statistically similar results to each other (p=0.139) and both had significantly lower bond strengths (p<0.05) than the other treatments. In conclusion, mechanical retention, either associated or not to chemical treatment, provided higher bond strength of the indirect composite resin to cpTi.

APA, Harvard, Vancouver, ISO, and other styles

22

Moriarty, Thomas F., Laurence Debefve, Ludovic Boure, Davide Campoccia, Urs Schlegel, and Robert G. Richards. "Influence of Material and Microtopography on the Development of Local Infection in vivo: Experimental Investigation in Rabbits." International Journal of Artificial Organs 32, no. 9 (September 2009): 663–70. http://dx.doi.org/10.1177/039139880903200916.

Full text

Abstract:

Polishing the surface of internal fracture fixation (IFF) implant materials can ease implant removal and reduce irritation to gliding tissues by reducing soft tissue adhesion and bony overgrowth. Thus, polishing the surface of these implants is expected to have significant clinical benefit in certain situations. The aim of the present study was to determine if polishing the surface of an IFF device influences susceptibility to infection. The local infection rate associated with 4-hole 2.0 mm Synthes® locking compression plates (LCPs) composed of clinically available commercially pure titanium (cpTi) and titanium aluminium niobium (TAN) in their standard microrough form was compared with that of their test polished equivalents and also to clinically available electropolished stainless steel (EPSS). The LCPs were fixed in locking mode onto the tibia of mature, female New Zealand White rabbits and a clinical strain of Staphylococcus aureus was added to the implantation site. Twenty eight days after surgery the rabbits were euthanized and assessed for infection. The rank order based on descending ID50 was; polished TAN, standard TAN, standard cpTi, EPSS and finally polished cpTi, however, the ID50 values did not differ greatly between the groups with the same material. Using the LCP model in locking mode, polishing the surface of both cpTi and TAN was not found to influence the susceptibility to infection in our animal model.

APA, Harvard, Vancouver, ISO, and other styles

23

Lane, Robert H., David E. Kelley, Elisa M. Gruetzmacher, and Sherin U. Devaskar. "Uteroplacental insufficiency alters hepatic fatty acid-metabolizing enzymes in juvenile and adult rats." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 280, no. 1 (January 1, 2001): R183—R190. http://dx.doi.org/10.1152/ajpregu.2001.280.1.r183.

Full text

Abstract:

Multiple adult morbidities are associated with intrauterine growth retardation (IUGR) including dyslipidemia. We hypothesized that uteroplacental insufficiency and subsequent IUGR in the rat would lead to altered hepatic fatty acid metabolism. To test this hypothesis, we quantified hepatic mRNA levels of acetyl-CoA carboxylase (ACC), carnitine palmitoyltransferase (CPTI), the β-oxidation-trifunctional protein (HADH), fasting serum triglycerides, and hepatic malonyl-CoA levels at different ages in control and IUGR rats. Fetal gene expression of all three enzymes was decreased. Juvenile gene expression of CPTI and HADH continued to be decreased, whereas gene expression of ACC was increased. Serum triglycerides were unchanged. A sex-specific response was noted in the adult rats. In males, serum triglycerides, hepatic malonyl-CoA levels, and ACC mRNA levels were significantly increased, and CPTI and HADH mRNA levels were significantly decreased. In contrast, the female rats demonstrated no significant changes in these variables. These results suggest that uteroplacental insufficiency leads to altered hepatic fatty acid metabolism that may contribute to the adult dyslipidemia associated with low birth weight.

APA, Harvard, Vancouver, ISO, and other styles

24

Scepanovic, Miodrag, Ljiljana Tihacek-Sojic, Milan Tasic, Radivoje Mitrovic, Aleksandar Todorovic, and Branka Trifkovic. "Finite element analysis in defining the optimal shape and safety factor of retentive clasp arms of removable partial denture." Vojnosanitetski pregled 70, no. 11 (2013): 999–1005. http://dx.doi.org/10.2298/vsp110526021s.

Full text

Abstract:

Bacground/Aim. Retentive force of removable partial denture (RPD) directly depends on elastic force of stretched retentive clasp arms (RCAs). During deflection RCA must have even stress distribution. Safety factor is the concept which can be applied in estimating durability and functionality of RCAs. This study was based on analyzing properties of clasps designed by conventional clasp wax profiles and defining the optimal shapes of RCAs for stress distribution and safety factor aspects. Methods. Computer-aided-design (CAD) models of RCAs with simulated properties of materials used for fabrication of RPD cobalt-chromium-molybdenum (CoCrMo) alloy, commercially pure titanium (CPTi) and polyacetale were analyzed. Results. The research showed that geometrics of Rapidflex profiles from the BIOS concept are defined for designing and modeling RCAs from CoCrMo alloys. I-Bar and Bonihard clasps made from CPTi might have the same design as Co- CrMo clasp only by safety factor aspect, but it is obvious that CPTi are much more flexible, so their shape must be more massive. Polyacetale clasps should not be fabricated by BIOS concept for CoCrMo alloy. A proof for that is the low value of safety factor. Conclusion. The BIOS concept should be used only for RCAs made of CoCrMo alloy and different wax profiles should be used for fabricating clasps of other investigated materials. The contribution of this study may be the improvement of present systems for defining the clasps shapes made from CoCrMo alloys. The more significant application is possibility of creating new concepts in defining shapes of RCA made from CPTi and polyacetale.

APA, Harvard, Vancouver, ISO, and other styles

25

SI, YANLING, WEILIN CHEN, ZHIMING ZHANG, and ENBO WANG. "THE INVESTIGATION ON ELECTRONIC PROPERTIES AND STABILITY OF POLYOXOMETALATES FUNCTIONALIZED BY ORGANOMETALLIC TITANIUM [CpTi · SiW9V3O40]4- BY DENSITY FUNCTIONAL THEORY." Journal of Theoretical and Computational Chemistry 08, no. 04 (August 2009): 773–81. http://dx.doi.org/10.1142/s0219633609005039.

Full text

Abstract:

The electronic structures of [ CpTi · SiW 9 V 3 O 40]4- constructed from Keggin–type polyoxometalates functionalized by CpTi 3+ group have been investigated by Density Functional Theory (DFT) methods. We discuss the relative stability affected by incorporating the CpTi 3+ group into the different sites of the [ SiW 9 V 3 O 40]7- framework on the basis of geometrical parameters, total bonding energies, fragment analysis and frontier molecular orbitals analysis. The calculated results indicated that the structure of the CpTi 3+ group coordinating to one terminal oxygen and two bridging oxygen atoms of the Keggin–type polyoxoanion (system a) is more stable than that to three bridging oxygen atoms (system b). In system a, Ti -Ob1, Ti -Ob2 and Ti - Ot1 are relatively shorter, and as result, it exhibits a more compact and nearly spherical structure of the well-known Keggin–type [ SiW 9 V 3 O 40]7-. Fragment analysis elucidates that there is deviation of ΔE B in systems a and b, which makes a significant contribution to the stability of system a. The stability of different isomeric forms of polyoxoanions appears to be a balance between the stabilizing ΔE O and ΔE E terms and the destabilizing ΔE P term. The relatively small energy differences (ΔE B = 4.26 eV ) between systems a and b arise directly from this balance. Frontier molecular orbitals analysis further confirms the stability order of systems a > b by the difference of the HOMO-LUMO energy gap.

APA, Harvard, Vancouver, ISO, and other styles

26

FAROOQ, AMEEQ, KASHIF MAIRAJ DEEN, MUHAMMAD RIZWAN, and AKHLAQ AHMAD. "SURFACE MODIFICATION OF COMMERCIALLY PURE TITANIUM BY GOLD-ION IRRADIATION FOR BIOMEDICAL APPLICATIONS." Surface Review and Letters 28, no. 09 (May 8, 2021): 2150069. http://dx.doi.org/10.1142/s0218625x21500694.

Full text

Abstract:

This research work’s aim was to enhance the biocompatibility of the commercially pure titanium (cpTi-2) surface via Au-ion irradiation. Various ion dosages, i.e. [Formula: see text] (Au-11), [Formula: see text] (Au-12) and [Formula: see text] (Au-13) ions[Formula: see text][Formula: see text][Formula: see text]cm[Formula: see text], were produced by exposing the polished cpTi-2 samples to Au-ion beam at room temperature. The surface topographic features of cpTi-2 and the effects of Au-ion-implanted surfaces were examined by atomic force microscopy and XRD analysis. Open-Circuit Potential (OCP), Potentiodynamic Polarization Scans (PPS) and Electrochemical Impedance Spectroscopy (EIS) were used to compare the electrochemical behavior of the cpTi-2 and Au-ion-implanted samples in Ringer’s lactate (RL) solution at 37∘C. The effects of Au-ion irradiation on the proliferation of mesenchymal stem cells (MSCs) were estimated during 24[Formula: see text]h and 48[Formula: see text]h of exposure. Based on the experimental results, Au-12 samples presented more positive OCP and lower corrosion rate in RL solution than the Au-11 and Au-13 samples. No significant change in the morphology of the MSCs was observed after exposure to the Au-ion-implanted samples. Similar to the controlled medium, the percentage of viability of the cells of the cells on Au-12 increased from 75% to 165% on the surface of Au-13 samples during 48[Formula: see text]h of incubation indicating the positive effects of Au-ion irradiation for biocompatibility.

APA, Harvard, Vancouver, ISO, and other styles

27

Miyamoto, Motoharu, Hiroshi Kono, Akihiko Yuda, Hisanoro Goto, Miho Machigashira, Seiji Ban, and Yuichi Izumi. "Early Cellular Responses of Osteoblast-Like Cells on Acid-Etching and Alkaline Treated Titanium." Key Engineering Materials 309-311 (May 2006): 403–6. http://dx.doi.org/10.4028/www.scientific.net/kem.309-311.403.

Full text

Abstract:

The effects of four surface modification (acid-etching, alkaline treatment, acid-etching /alkaline treatment, and sandblasting) of commercially pure titanium (cpTi) on the early cellular responses of osteoblast-like MC3T3-E1 cells were investigated. MTT assay was used to measure the levels of cell attachment to the different surface specimens after 1-, 2-, and 3-hr cell incubation. All data were submitted to two-way analysis of variance. Cell morphology was observed by scanning electron microscopy (SEM). Results showed that initial adhesion of osteoblast-like cells was independent on the surface of cpTi modified with different method.

APA, Harvard, Vancouver, ISO, and other styles

28

Cottingham, Katie. "Proteomics Projects: NCI CPTI: Technologies for proteomics." Journal of Proteome Research 5, no. 10 (October 2006): 2502. http://dx.doi.org/10.1021/pr062763c.

Full text

APA, Harvard, Vancouver, ISO, and other styles

29

Fu, Qi, Robert A. McKnight, Xing Yu, Laiyi Wang, Christopher W. Callaway, and Robert H. Lane. "Uteroplacental insufficiency induces site-specific changes in histone H3 covalent modifications and affects DNA-histone H3 positioning in day 0 IUGR rat liver." Physiological Genomics 20, no. 1 (December 15, 2004): 108–16. http://dx.doi.org/10.1152/physiolgenomics.00175.2004.

Full text

Abstract:

Uteroplacental insufficiency and subsequent intrauterine growth retardation (IUGR) increase the risk of adult onset insulin resistance and dyslipidemia in humans and rats. IUGR rats are further characterized by postnatal alterations in hepatic PPAR-γ coactivator (PGC-1) and carnitine-palmitoyl-transferase I (CPTI) expression, as well as overall hyperacetylation of histone H3. However, it is unknown whether the histone H3 hyperacetylation is site specific or relates to the changes in gene expression previously described in IUGR rats. We therefore hypothesized that uteroplacental insufficiency causes site-specific modifications in hepatic H3 acetylation and affects the association of acetylated histone H3 with PGC-1 and CPTI promoter sequences. Uteroplacental insufficiency was used to produce asymmetrical IUGR rats. IUGR significantly increased acetylation of H3 lysine-9 (H3/K9), lysine-14 (H3/K14), and lysine-18 (H3/K18) at day 0 of life, and these changes occurred in association with decreased nuclear protein levels of histone deacetylase 1 (HDAC1) and HDAC activity. Chromatin immunoprecipitation using acetyl-H3/K9 antibody and day 0 chromatin revealed that uteroplacental insufficiency affected the association between acetylated H3/K9 and the promoters of PGC-1 and CPTI, respectively, in IUGR liver. At day 21 of life, the neonatal pattern of H3 hyperacetylation persisted only in the IUGR males. We conclude that uteroplacental insufficiency increases H3 acetylation in a site-specific manner in IUGR liver and that these changes persist in male IUGR animals. The altered association of the PGC-1 and CPTI promoters with acetylated H3/K9 correlates with previous reports of IUGR altering the expression of these genes. We speculate that in utero alterations of chromatin structure contribute to fetal programming.

APA, Harvard, Vancouver, ISO, and other styles

30

Lohberger, Birgit, Nicole Eck, Dietmar Glaenzer, Heike Kaltenegger, and Andreas Leithner. "Surface Modifications of Titanium Aluminium Vanadium Improve Biocompatibility and Osteogenic Differentiation Potential." Materials 14, no. 6 (March 23, 2021): 1574. http://dx.doi.org/10.3390/ma14061574.

Full text

Abstract:

Osteogenic cells are strongly influenced in their behaviour by the surface properties of orthopaedic implant materials. Mesenchymal stem and progenitor cells (MSPCs) migrate to the bone–implant interface, adhere to the material surface, proliferate and subsequently differentiate into osteoblasts, which are responsible for the formation of the bone matrix. Five surface topographies on titanium aluminium vanadium (TiAl6V4) were engineered to investigate biocompatibility and adhesion potential of human osteoblasts and the changes in osteogenic differentiation of MSPCs. Elemental analysis of TiAl6V4 discs coated with titanium nitride (TiN), silver (Ag), roughened surface, and pure titanium (cpTi) surface was analysed using energy-dispersive X-ray spectroscopy and scanning electron microscopy. In vitro cell viability, cytotoxicity, adhesion behaviour, and osteogenic differentiation potential were measured via CellTiter-Glo, CytoTox, ELISA, Luminex® technology, and RT-PCR respectively. The Ag coating reduced the growth of osteoblasts, whereas the viability of MSPCs increased significantly. The roughened and the cpTi surface improved the viability of all cell types. The additive coatings of the TiAl6V4 alloy improved the adhesion of osteoblasts and MSPCs. With regard to the osteogenic differentiation potential, an enhanced effect has been demonstrated, especially in the case of roughened and cpTi coatings.

APA, Harvard, Vancouver, ISO, and other styles

31

Voltrova, Barbora, Vojtech Hybasek, Veronika Blahnova, Josef Sepitka, Vera Lukasova, Karolina Vocetkova, Vera Sovkova, et al. "Different diameters of titanium dioxide nanotubes modulate Saos-2 osteoblast-like cell adhesion and osteogenic differentiation and nanomechanical properties of the surface." RSC Advances 9, no. 20 (2019): 11341–55. http://dx.doi.org/10.1039/c9ra00761j.

Full text

APA, Harvard, Vancouver, ISO, and other styles

32

Kono, Hiroshi, Motoharu Miyamoto, Hideo Sato, and Seiji Ban. "Apatite Coating on Ti Surface Using Alternate Soaking Process." Key Engineering Materials 309-311 (May 2006): 679–84. http://dx.doi.org/10.4028/www.scientific.net/kem.309-311.679.

Full text

Abstract:

An apatite coating by an alternate soaking was undertaken on commercially pure titanium (cpTi) treated with concentrated acid and alkaline solutions. X-ray diffraction (XRD) patterns indicated that the diffraction peak intensities assigned to hydroxyapatite (HAp) increased with the reaction cycle. Fourier transform infrared spectroscopic study (FTIR) showed similar results with XRD. Scanning electron micrographs (SEM) of the deposits showed no observable change after the vacuum firing. It was confirmed that the reaction cycle greatly affected on the deposited amount of apatite on cpTi plates using the alternate soaking and the vacuum firing erased TiH2 formed during the acid etching, but was irrelevant to the crystallinity of the apatite.

APA, Harvard, Vancouver, ISO, and other styles

33

Morillo, Angel, Juan Chirinos, Tamara Rajmankina, Darmenia Ibarra, Jose Arevalo, and Alvaro Parada. "Ethene Polymerisation with Titanium Half-Sandwich Amido-Complexes." Journal of Chemical Research 2003, no. 4 (April 2003): 238–39. http://dx.doi.org/10.3184/030823403103173633.

Full text

APA, Harvard, Vancouver, ISO, and other styles

34

Cambie, RC, JM Coddington, JBJ Milbank, MG Pausler, JJ Rustenhoven, PS Rutledge, GL Shaw, and PI Sinkovich. "Stereoselective Titanium-Mediated Aldol Reactions of Menthyl Acetate Enolates, and Allylations With Benzaldehyde." Australian Journal of Chemistry 46, no. 5 (1993): 583. http://dx.doi.org/10.1071/ch9930583.

Full text

Abstract:

The enolates of (+)- and (-)- menthyl acetate, formed by transmetallation from lithium with chloro ( cyclopentadienyl )bis(1,2:5,6-di-O-isopropylidene-α-D-glucofuranosato-O3)titanium [ CpTi (DAGO)2Cl] (1), react with benzaldehyde from the Re-face with high stereoselectivities (95 and 92% diastereomeric excess) when 12-crown-4 is used during the transmetallation . Use of the chiral cyclopentadienyltitanium-α,α,αα,αα-tetraaryl-2,2-dimethyl-1,3-dioxolan-4,5-dimethanol (TADDOL) complex (3) gives lower diastereoselectivities (58 and 40%) but the Si -face of benzaldehyde is attacked preferentially. Transmetallation of the 2-methylprop-2-enyl moiety to CpTi (DAGO)2Cl gives an allyltitanium reagent (2) which reacts with benzaldehyde from the Re-face with 88% enantiomeric excess.

APA, Harvard, Vancouver, ISO, and other styles

35

Muñoz-Castro, A. E., R. López-Callejas, E. E. Granda-Gutiérrez, R. Valencia-Alvarado, S. R. Barocio, R. Peña-Eguiluz, A. Mercado-Cabrera, and A. de la Piedad Beneitez. "Ion implantation of oxygen and nitrogen in CpTi." Progress in Organic Coatings 64, no. 2-3 (February 2009): 259–63. http://dx.doi.org/10.1016/j.porgcoat.2008.08.021.

Full text

APA, Harvard, Vancouver, ISO, and other styles

36

Gindelberger, D. E. "[(CpTi)6(μ3-Te)6(μ3-O)2]." Acta Crystallographica Section C Crystal Structure Communications 52, no. 10 (October 15, 1996): 2493–95. http://dx.doi.org/10.1107/s0108270196004854.

Full text

APA, Harvard, Vancouver, ISO, and other styles

37

Eaton, S., K. Fukumoto, N. P. Duran, A. Pierro, L. Spitz, P. A. Quant, and K. Bartlett. "CPTI and control of myocardial β-oxidation flux." Biochemical Society Transactions 29, no. 1 (February 1, 2001): A1. http://dx.doi.org/10.1042/bst029a001c.

Full text

APA, Harvard, Vancouver, ISO, and other styles

38

Das, Indranee, Shreyasi Chattopadhyay, Arnab Mahato, Biswanath Kundu, and Goutam De. "Fabrication of a cubic zirconia nanocoating on a titanium dental implant with excellent adhesion, hardness and biocompatibility." RSC Advances 6, no. 64 (2016): 59030–38. http://dx.doi.org/10.1039/c6ra10661g.

Full text

Abstract:

Crystalline cubic zirconia nanocoating on cpTi with enhanced surface hardness, durability and biocompatibility, useful as an advanced oral implant was fabricated by applying layer-by-layer dip-coating at low annealing temperature.

APA, Harvard, Vancouver, ISO, and other styles

39

Fu, Ming-hua H., Amy C. Maher, Mazen J. Hamadeh, Changhua Ye, and Mark A. Tarnopolsky. "Exercise, sex, menstrual cycle phase, and 17β-estradiol influence metabolism-related genes in human skeletal muscle." Physiological Genomics 40, no. 1 (December 2009): 34–47. http://dx.doi.org/10.1152/physiolgenomics.00115.2009.

Full text

Abstract:

Higher fat and lower carbohydrate and amino acid oxidation are observed in women compared with men during endurance exercise. We hypothesized that the observed sex difference is due to estrogen and that menstrual cycle phase or supplementation of men with 17β-estradiol (E2) would coordinately influence the mRNA content of genes involved in lipid and/or carbohydrate metabolism in skeletal muscle. Twelve men and twelve women had muscle biopsies taken before and immediately after 90 min of cycling at 65% peak oxygen consumption (V̇o2peak). Women were studied in the midfollicular (Fol) and midluteal (Lut) phases, and men were studied after 8 days of E2 or placebo supplementation. Targeted RT-PCR was used to compare mRNA content for genes involved in transcriptional regulation and lipid, carbohydrate, and amino acid metabolism. Sex was the greatest predictor of substrate metabolism gene content. Sex affected the mRNA content of FATm, FABPc, SREBP-1c, mtGPAT, PPARδ, PPARα, CPTI, TFP-α, GLUT4, HKII, PFK, and BCOADK ( P < 0.05). E2 administration significantly ( P < 0.05) affected the mRNA content of PGC-1α, PPARα, PPARδ, TFP-α, CPTI, SREBP-1c, mtGPAT, GLUT4, GS-1, and AST. Acute exercise increased the mRNA abundance for PGC-1α, HSL, FABPc, CPTI, GLUT4, HKII, and AST ( P < 0.05). Menstrual cycle had a small effect on PPARδ, GP, and glycogenin mRNA content. Overall, women have greater mRNA content for several genes involved in lipid metabolism, which is partially due to an effect of E2.

APA, Harvard, Vancouver, ISO, and other styles

40

Abey, Savithri, Mathew T. Mathew, Damian J. Lee, Kent L. Knoernschild, Markus A. Wimmer, and Cortino Sukotjo. "Electrochemical Behavior of Titanium in Artificial Saliva: Influence of pH." Journal of Oral Implantology 40, no. 1 (February 1, 2014): 3–10. http://dx.doi.org/10.1563/aaid-joi-d-11-00054.

Full text

Abstract:

Titanium is the most common material chosen for dental implants because it is highly corrosion resistant because it constantly reforms a protective passive film layer. The formation and composition of the passive film layer is dependent on the environmental conditions. If the stable oxide layer is damaged, the titanium surface underneath can corrode. The purpose of this study was to determine if basic corrosion of commercially pure titanium (CpTi) alloy in artificial saliva was affected by pH and to understand the corrosion kinetics/mechanisms of CpTi as a function of pH. In this study, titanium alloy discs were subjected to corrosion tests. Before the tests, all samples were cleaned and polished using standard metallographic preparation methods. Artificial saliva was used as the testing medium. The following pH values were tested: 3.0, 4.5, 6.0, 6.5, 7.5, and 9.0. Different pH values were achieved by adding lactic acid (acidic) or NaOH (basic) in appropriate amounts. Potentiodynamic curves indicated behavior change at each pH. In addition, the corrosion current density value determined from the potentiodynamic curve exhibited the poorest corrosion resistance for pH 7.5. The Nyquist plot (from the electrochemical impedance spectroscopy results) indicated that pH 7.5 had the poorest resistance. Scanning electron microscopy images indicated that pH levels of 6.5, 7.5, and 9.0 had considerable surface corrosion. The results showed that the media's pH significantly influenced the corrosion behavior of CpTi. The poor corrosion behavior at the neutral pHs invites some concerns and highlights the need for further study.

APA, Harvard, Vancouver, ISO, and other styles

41

Paulitsch-Fuchs, Astrid H., Lukas Wolrab, Nicole Eck, Nigel P. Dyer, Benjamin Bödendorfer, and Birgit Lohberger. "TiAl6V4 Alloy Surface Modifications and Their Impact on Biofilm Development of S. aureus and S. epidermidis." Journal of Functional Biomaterials 12, no. 2 (May 18, 2021): 36. http://dx.doi.org/10.3390/jfb12020036.

Full text

Abstract:

One of the most serious complications following joint replacement surgeries are periprosthetic infections (PIs) arising from the adhesion of bacteria to the artificial joint. Various types of titanium–aluminum–vanadium (TiAl6V4) alloy surface modifications (coatings with silver (Ag), titanium nitride (TiN), pure titanium (cpTi), combinations of cpTi and hydroxyapatite (HA), combinations of cpTi and tricalcium phosphate (TCP), and a rough-blasted surface of TiAl6V4) have been investigated to assess their effects on biofilm development. Biofilms were grown, collected, and analyzed after 48 h to measure their protein and glucose content and the cell viability. Biofilm-associated genes were also monitored after 48 h of development. There was a distinct difference in the development of staphylococcal biofilms on the surfaces of the different types of alloy. According to the findings of this study, the base alloy TiAl6V4 and the TiN-coated surface are the most promising materials for biofilm reduction. Rough surfaces are most favorable when it comes to bacterial infections because they allow an easy attachment of pathogenic organisms. Of all rough surfaces tested, rough-blasted TiAl6V4 was the most favorable as an implantation material; all the other rough surfaces showed more distinct signs of inducing the development of biofilms which displayed higher protein and polysaccharide contents. These results are supported by RT-qPCR measurements of biofilm associated genes for Staphylococcus aureus (icaA, icaC, fnbA, fnbB, clfB, atl) and Staphylococcus epidermidis (atle, aap).

APA, Harvard, Vancouver, ISO, and other styles

42

Ferreira, Olívia Galvão Lucena, Silvana Carneiro Maciel, Antonia Oliveira Silva, Roseane Christina da Nova Sá, and Maria Adelaide Silva P. Moreira. "Significados atribuídos ao envelhecimento: idoso, velho e idoso ativo." Psico-USF 15, no. 3 (December 2010): 357–64. http://dx.doi.org/10.1590/s1413-82712010000300009.

Full text

Abstract:

O presente estudo teve como objetivo apreender os significados atribuídos ao envelhecimento elaborados por idosos funcionalmente independentes. Utilizando o aporte teórico das representações sociais, tratou-se de priorizar as falas dos participantes, a fim de salientar as dimensões simbólicas acerca do envelhecimento. O estudo foi realizado na Unidade de Saúde da Família (USF) localizada no bairro Alto do Mateus, na cidade de João Pessoa, PB. A amostra foi constituída por 100 idosos funcionalmente independentes, com idades entre 60 e 93 anos. Como instrumentos para a coleta de dados foram utilizados o MIF e o Teste de Associação Livre de Palavras, sendo os resultados submetidos à análise do software Tri-Deux-Mots. Com base nos resultados, observou-se que os estímulos idoso e velho foram associados a aspectos com conotações mais negativas. No entanto, para o estímulo idoso ativo, embora existissem as conotações negativas, os significados positivos foram mais enfocados pelos idosos investigados.

APA, Harvard, Vancouver, ISO, and other styles

43

Biasetto, Lisa, and Hamada Elsayed. "Sphene silicate ceramic coatings on cpTi substrates: Process upgrade." Surface and Coatings Technology 321 (July 2017): 416–24. http://dx.doi.org/10.1016/j.surfcoat.2017.05.018.

Full text

APA, Harvard, Vancouver, ISO, and other styles

44

Eliopoulos, Dimitris, Spiros Zinelis, and Triantafillos Papadopoulos. "Porosity of cpTi casting with four different casting machines." Journal of Prosthetic Dentistry 92, no. 4 (October 2004): 377–81. http://dx.doi.org/10.1016/j.prosdent.2004.07.006.

Full text

APA, Harvard, Vancouver, ISO, and other styles

45

Kaczorowski, J. "Pediatrics in the Community: Community Pediatrics Training Initiative (CPTI)." Pediatrics in Review 29, no. 1 (January 1, 2008): 31–32. http://dx.doi.org/10.1542/pir.29-1-31.

Full text

APA, Harvard, Vancouver, ISO, and other styles

46

Kiran, A., T. S. Kumar, Rutvi Sanghavi, Mukesh Doble, and Seeram Ramakrishna. "Antibacterial and Bioactive Surface Modifications of Titanium Implants by PCL/TiO2 Nanocomposite Coatings." Nanomaterials 8, no. 10 (October 20, 2018): 860. http://dx.doi.org/10.3390/nano8100860.

Full text

Abstract:

Surface modification of biomedical implants is an established strategy to improve tissue regeneration, osseointegration and also to minimize the bacterial accumulation. In the present study, electrospun poly(ε-caprolactone)/titania (PCL/TiO2) nanocomposite coatings were developed on commercially pure titanium (cpTi) substrates for an improved biological and antibacterial properties for bone tissue engineering. TiO2 nanoparticles in various amounts (2, 5, and 7 wt %) were incorporated into a biodegradable PCL matrix to form a homogeneous solution. Further, PCL/TiO2 coatings on cpTi were obtained by electrospinning of PCL/TiO2 solution onto the substrate. The resulted coatings were structurally characterized and inspected by employing scanning electron microscope (SEM), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy. Given the potential biological applications of PCL/TiO2 coated cpTi substrates, the apatite-forming capacity was examined by immersing in simulated body fluid (SBF) for upto 21 days. Biocompatibility has been evaluated through adhesion/proliferation of hFOB osteoblast cell lines and cytotoxicity by MTT assay. Antimicrobial activity of PCL/TiO2 nanocomposites has been tested using UV light against gram-positive Staphylococcus aureus (S.aureus). The resulting surface displays good bioactive properties against osteoblast cell lines with increased viability of 40% at day 3 and superior antibacterial property against S.aureus with a significant reduction of bacteria to almost 76%. Surface modification by PCL/TiO2 nanocomposites makes a viable approach for improving dual properties, i.e., biological and antibacterial properties on titanium implants which might be used to prevent implant-associated infections and promoting cell attachment of orthopedic devices at the same time.

APA, Harvard, Vancouver, ISO, and other styles

47

Martins, Ivone Dos Santos. "Deprescribing no idoso." Revista Portuguesa de Clínica Geral 29, no. 1 (January 1, 2013): 66–69. http://dx.doi.org/10.32385/rpmgf.v29i1.11048.

Full text

APA, Harvard, Vancouver, ISO, and other styles

48

Ruwer, Sheelen Larissa, Angela Garcia Rossi, and Larissa Fortunato Simon. "Equilíbrio no idoso." Revista Brasileira de Otorrinolaringologia 71, no. 3 (June 2005): 298–303. http://dx.doi.org/10.1590/s0034-72992005000300006.

Full text

Abstract:

Com o passar dos anos, o organismo humano passa por um processo natural de envelhecimento, gerando modificações funcionais e estruturais no organismo. As vias responsáveis pelo equilíbrio corporal também sofrem com o processo do envelhecimento, gerando grande impacto para os idosos. OBJETIVO: Assim, o presente trabalho objetiva estudar a função vestibular de idosos em função das queixas de tontura, zumbido e dificuldade auditiva. FORMA DE ESTUDO: Coorte transversal. MATERIAL E MÉTODO: Foram avaliados 80 idosos de dois grupos distintos: Grupo A - composto por 38 mulheres e dois homens pertencentes a um grupo de terceira idade; e Grupo B: composto por 35 mulheres e cinco homens com queixas efetivas de alterações do equilíbrio corporal. RESULTADO: Os idosos foram submetidos à anamnese, sendo investigados prioritariamente aspectos relativos a tontura, zumbido e dificuldade auditiva; e à avaliação vestibular, realizada por intermédio do sistema computadorizado de vecto-eletronistagmografia SCV 5.0. Os resultados demonstram uma diferença estatisticamente significante entre os grupos, no que diz respeito às queixas de tontura e zumbido, as quais prevaleceram nos indivíduos do grupo B. Na hipótese diagnóstica predominante à vecto-eletronistagmografia computadorizada constatou-se que a maioria dos idosos apresentou diagnóstico normal, porém verificou-se a prevalência de alterações vestibulares nos idosos como Síndrome vestibular periférica deficitária e Síndrome vestibular periférica irritativa. Não se observou sinais patognomônicos de alterações centrais ao exame vestibular. CONCLUSÃO: Concluiu-se que as alterações vestibulares à Vecto-eletronistagmografia, em função das queixas de tontura, zumbido e dificuldade auditiva, são numericamente semelhantes no Grupo de Terceira Idade e no Grupo de idosos com queixas efetivas de alterações do equilíbrio corporal.

APA, Harvard, Vancouver, ISO, and other styles

49

SOUZA, JOSÉ ANTONIO GOMES DE, and ANTONIO CARLOS R. G. IGLESIAS. "Trauma no idoso." Revista da Associação Médica Brasileira 48, no. 1 (March 2002): 79–86. http://dx.doi.org/10.1590/s0104-42302002000100037.

Full text

APA, Harvard, Vancouver, ISO, and other styles

50

Zanini, Rachel Schlindwein. "Demência no idoso." Revista Neurociências 18, no. 2 (March 31, 2001): 220–26. http://dx.doi.org/10.34024/rnc.2010.v18.8482.

Full text

Abstract:

O envelhecimento revela mudanças no indivíduo (em seus aspectos psicológicos, sociais, físicos e neuropsicológicos) e no ambiente que o cerca. Tendo alta incidência no idoso, a depressão e as demências, podem trazer déficits de cognição, de memória, linguagem, funções executivas, além de gnosias e praxias, interferindo na autonomia, no desempenho social ou profissional do indivíduo. Assim, nota-se a importância de estudos sobre este tema. Objetivo. Revisão da literatura pertinente acerca dos aspectos neuropsicológicos do idoso e sua avaliação neuropsicológica, especialmente relacionados às demências, sugerindo procedimentos úteis no atendimento deste paciente. Método. Revisão de literatura. Pesquisa nas seguintes bases de dados Bireme, Scielo e Pubmed. E também livros e teses. Conclusão. A avaliação neuropsicológica é indicada, principalmente na identificação de declínio cognitivo no idoso, na avaliação dos prejuízos de áreas cerebrais em alterações neurológicas, no diagnóstico diferencial de síndrome psicológica/psiquiátrica e neurológica; norteando uma intervenção reabilitadora.

APA, Harvard, Vancouver, ISO, and other styles

Journal articles on the topic 'Idoso e a CPTI'

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles