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Journal articles on the topic 'IGF1'

Author: Grafiati

Published: 4 June 2021

Last updated: 26 July 2025

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1

Juengel, Jennifer L., Lisa J. Haydon, Brigitta Mester, Brian P. Thomson, Michael Beaumont, and Douglas C. Eckery. "The role of IGFs in the regulation of ovarian follicular growth in the brushtail possum (Trichosurus vulpecula)." REPRODUCTION 140, no. 2 (2010): 295–303. http://dx.doi.org/10.1530/rep-10-0142.

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IGFs are known to be key regulators of ovarian follicular growth in eutherian mammals, but little is known regarding their role in marsupials. To better understand the potential role of IGFs in the regulation of follicular growth in marsupials, expression of mRNAs encoding IGF1, IGF2, IGF1R, IGF-binding protein 2 (IGFBP2), IGFBP4 and IGFBP5 was localized by in situ hybridization in developing ovarian follicles of the brushtail possum. In addition, the effects of IGF1 and IGF2 on granulosa cell function were tested in vitro. Both granulosa and theca cells synthesize IGF mRNAs, with the theca ex

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Ndandala, Charles Brighton, Qi Zhou, Zhiyuan Li, Yuwen Guo, Guangli Li, and Huapu Chen. "Identification of Insulin-like Growth Factor (IGF) Family Genes in the Golden Pompano, Trachinotus ovatus: Molecular Cloning, Characterization and Gene Expression." International Journal of Molecular Sciences 25, no. 5 (2024): 2499. http://dx.doi.org/10.3390/ijms25052499.

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Insulin-like growth factors (IGFs) are hormones that primarily stimulate and regulate animal physiological processes. In this study, we cloned and identified the open reading frame (ORF) cDNA sequences of IGF family genes: the insulin-like growth factor 1 (IGF1), insulin-like growth factor 2 (IGF2), and insulin-like growth factor 3 (IGF3). We found that IGF1, IGF2, and IGF3 have a total length of 558, 648, and 585 base pairs (bp), which encoded a predicted protein with 185, 215, and 194 amino acids (aa), respectively. Multiple sequences and phylogenetic tree analysis showed that the mature gol

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Frystyk, Jan, Anders J. Schou, Carsten Heuck, et al. "Prednisolone reduces the ability of serum to activate the IGF1 receptor in vitro without affecting circulating total or free IGF1." European Journal of Endocrinology 168, no. 1 (2013): 1–8. http://dx.doi.org/10.1530/eje-12-0518.

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ObjectiveEnd-point bioassays based on thymidine or sulfate incorporation have demonstrated that glucocorticoid (GC) treatment inhibits serum IGF1 action, but the mechanism is unknown as serum IGF1 concentrations have been reported to either increase or remain unchanged.AimTo investigate whether GC treatment affects the ability of serum to activate the IGF1 receptor (IGF1R) in vitro (i.e. bioactive IGF1), using a specific cell-based IGF1 kinase receptor activation assay.Subjects and methodsTwenty children with stable asthma (age 7.7–13.8 years) treated for 1 week with 5 mg prednisolone in a ran

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Kharkova, A., and O. Minchenko. "Factors and their receptor genes in glioma cells with suppressed function of ERN 1 enzyme upon glucose deprivation." Bulletin of Taras Shevchenko National University of Kyiv. Series: Problems of Physiological Functions Regulation 20, no. 1 (2016): 44–49. http://dx.doi.org/10.17721/2616_6410.2016.20.44-49.

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It was shown that the expression level of insulin like growth factors (IGF1 and IGF2) genes is decreased, but IGF receptor (IRF1R) gene is significantly increased in U87 glioma cells with suppressed activity of the sensor and signaling enzyme ERN1. In U87 glioma cells the expression level of IGF1 gene is decreased but IGF2 and IGF1R do not change significantly upon glucose deprivation condition. The inhibition of ERN1 functional activity does not affect the sensitivity of IGF1 and IGF1R gene expressions to glucose deprivation but the inhibition of ERN1 eliminates the effect of glucose deprivat

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Chisalita, S. I., G. S. Johansson, E. Liefvendahl, K. Bäck, and H. J. Arnqvist. "Human aortic smooth muscle cells are insulin resistant at the receptor level but sensitive to IGF1 and IGF2." Journal of Molecular Endocrinology 43, no. 6 (2009): 231–39. http://dx.doi.org/10.1677/jme-09-0021.

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Whether insulin, at physiological concentrations, has direct effects on vascular smooth muscle cells (VSMCs) remains controversial. Our aim was to characterize the mechanism for insulin resistance in VSMCs. For comparison, the effects of IGF1 and IGF2 were also studied. Cultured human aortic smooth muscle cells (HASMC) were used. Receptor mRNA was analyzed by quantitative reverse transcription PCR and receptor protein by ELISA and western blot. Biological effects were studied by thymidine incorporation and glucose accumulation. In HASMC, both mRNA and protein expression of IGF1 receptors (IGF1

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Gatford, K. L., G. K. Heinemann, S. D. Thompson, et al. "Circulating IGF1 and IGF2 and SNP genotypes in men and pregnant and non-pregnant women." Endocrine Connections 3, no. 3 (2014): 138–49. http://dx.doi.org/10.1530/ec-14-0068.

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Circulating IGFs are important regulators of prenatal and postnatal growth, and of metabolism and pregnancy, and change with sex, age and pregnancy. Single-nucleotide polymorphisms (SNPs) in genes coding for these hormones associate with circulating abundance of IGF1 and IGF2 in non-pregnant adults and children, but whether this occurs in pregnancy is unknown. We therefore investigated associations of plasma IGF1 and IGF2 with age and genotype at candidate SNPs previously associated with circulating IGF1, IGF2 or methylation of the INS–IGF2–H19 locus in men (n=134), non-pregnant women (n=74) a

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Díaz del Moral, Sandra, Maha Benaouicha, Ramón Muñoz-Chápuli, and Rita Carmona. "The Insulin-like Growth Factor Signalling Pathway in Cardiac Development and Regeneration." International Journal of Molecular Sciences 23, no. 1 (2021): 234. http://dx.doi.org/10.3390/ijms23010234.

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Insulin and Insulin-like growth factors (IGFs) perform key roles during embryonic development, regulating processes of cell proliferation and survival. The IGF signalling pathway comprises two IGFs (IGF1, IGF2), two IGF receptors (IGFR1, IGFR2), and six IGF binding proteins (IGFBPs) that regulate IGF transport and availability. The IGF signalling pathway is essential for cardiac development. IGF2 is the primary mitogen inducing ventricular cardiomyocyte proliferation and morphogenesis of the compact myocardial wall. Conditional deletion of the Igf1r and the insulin receptor (Insr) genes in the

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Mashayekhi, Farhad, Somayeh Shabani, and Zivar Salehi. "Comparison of serum IGF1, IGF2 and IGFBP1-6 concentration in the children with different stages of autism spectrum disorders." Archives of Psychiatry and Psychotherapy 24, no. 3 (2022): 20–24. http://dx.doi.org/10.12740/app/144882.

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Aim of the studyAutism spectrum disorder (ASD) is a neurodevelopmental condition considered by early-onset difficulties in social communication. Insulin-like growth factors (IGFs) play crucial roles in synapse formation. Most of circulating IGFs are bound to IGF-binding proteins (IGFBPs) that modify IGF action. IGFBPs prolong the plasma half-life of IGFs. In this project we studied the association of IGF1/2 and IGFBP1-6 serum concentration with the severity of ASD (Levels 1-3; Mild, Moderate and severe, respectively).Subject or material and methodsA hundred and eighty patients with ASD (Mild;

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Minchenko, Dmytro O., A. P. Kharkova, O. V. Halkin, L. L. Karbovskyi, and O. H. Minchenko. "Effect of hypoxia on the expression of genes encoding insulin-like growth factors and some related proteins in U87 glioma cells without IRE1 function." Endocrine Regulations 50, no. 2 (2016): 43–54. http://dx.doi.org/10.1515/enr-2016-0008.

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AbstractObjective. The aim of the present study was to investigate the effect of hypoxia on the expression of genes encoding insulin-like growth factors (IGF1 and IGF2), their receptor (IGF1R), binding protein-4 (IGFBP4), and stanniocalcin 2 (STC2) in U87 glioma cells in relation to inhibition of endoplasmic reticulum stress signaling mediated by IRE1 (inositol requiring enzyme 1) for evaluation of their possible significance in the control of tumor growth. Methods. The expression of IGF1, IGF2, IGF1R, IGFBP4, and STC2 genes in U87 glioma cells transfected by empty vector pcDNA3.1 (control) an

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Hernández, Rocío, Gimena Gomez Castro, Fernanda Mariel Rodriguez, Luciano Casas, and Enrique Portiansky. "Insulin-like growth factors in cat placenta." Analecta Veterinaria 44 (February 18, 2025): 090. https://doi.org/10.24215/15142590e090.

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Placental development involves maternal-fetal signalling events, and it is an under-researched topic in feline reproduction. Few studies of endotheliochorial placentas deal with insulin-like growth factors (IGFs), which are among the principal developmental regulators. While placental expression of IGFs and type 1 IGF receptor (IGF1R) has already been reported in bitches, data regarding the IGF system in queens are limited to uterine tissues. This study aimed to detect IGF1, IGF2, and IGF1R in fetal and maternal placental structures. Samples from twenty-three placentas were classified into one

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Rebouças, Emanuela L., José J. N. Costa, Maria J. Passos, et al. "Expression levels of mRNA for insulin-like growth factors 1 and 2, IGF receptors and IGF binding proteins in in vivo and in vitro grown bovine follicles." Zygote 22, no. 4 (2013): 521–32. http://dx.doi.org/10.1017/s0967199413000166.

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SummaryThis study investigated mRNA levels for insulin-like growth factors (IGFs) IGF1 (IGF-I) and IGF2 (IGF-II), IGF receptors (IGF1R and IGF2R), and binding proteins (IGFBP-1, IGFBP-2. IGFBP-3, IGFBP-4, IGFBP-5 and IGFBP-6) in bovine follicles of 0.2, 0.5 or 1.0 mm in diameter. mRNA expression levels in in vitro cultured follicles that reached approximately 0.5 mm were compared with that of in vivo grown follicles. IGF1R and IGF2R expression levels in 0.5 mm in vivo follicles were higher than in 1.0 or 0.2 mm follicles, respectively. IGFBP-1, IGFBP-2. IGFBP-3, IGFBP-4, IGFBP-5 and IGFBP-6 sh

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Wang, Yan, Xuefeng Han, Zhiliang Tan, Jinhe Kang, and Zheng Wang. "Rumen-Protected Glucose Stimulates the Insulin-Like Growth Factor System and mTOR/AKT Pathway in the Endometrium of Early Postpartum Dairy Cows." Animals 10, no. 2 (2020): 357. http://dx.doi.org/10.3390/ani10020357.

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This study aimed to elucidate the effects of a dietary rumen-protected glucose (RPG) addition on uterine involution through the analysis of an insulin-like growth factor (IGF) system and associated pathways in the post-natal endometrium. Twelve Holstein cows were assigned equally to two groups: a control group (CT) and an RPG group (200 g of RPG per cow per day). The plasma content of insulin-like growth factor 1 (IGF1) was determined by using the ELISA method. Expressions of IGF members, the matrix metalloproteinase, protein kinase B (AKT)/mechanistic target of rapamycin complex1 (mTOR) signa

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Trajkovic-Arsic, Marija, Evdokia Kalideris, and Jens T. Siveke. "The role of insulin and IGF system in pancreatic cancer." Journal of Molecular Endocrinology 50, no. 3 (2013): R67—R74. http://dx.doi.org/10.1530/jme-12-0259.

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The importance of the IGF system in carcinogenesis has been established for many solid cancers. It is well known that individuals with higher circulating levels of the IGF1 ligand present an increased risk of cancer. However, therapies with monoclonal antibodies targeting the IGF1 receptor (IGF1R) have been largely unsuccessful. One of the potential reasons for this failure is the existence of the highly homologous insulin receptor (IR), which appears to be at least equally efficient as the IGF1R in the transition of mitogenic signals to the nucleus and promotion of cell growth. Furthermore, I

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Ndandala, Charles Brighton, Yuwen Guo, Zhimin Ju, Muhammad Fachri, Happiness Moses Mwemi, and Huapu Chen. "Integrated lncRNA and mRNA Transcriptome Analyses of IGF1 and IGF2 Stimulated Ovaries Reveal Genes and Pathways Potentially Associated with Ovarian Development and Oocyte Maturation in Golden Pompano (Trachinotus ovatus)." Animals 15, no. 8 (2025): 1134. https://doi.org/10.3390/ani15081134.

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Insulin-like growth factors (IGFs) play crucial roles in the regulation of animal growth and reproduction. However, the functional and regulatory mechanisms underlying ovarian growth and oocyte maturation in teleosts remain unclear. In this study, the expression profiles of lncRNAs and mRNAs were analyzed in the ovaries of golden pompano (Trachinotus ovatus) treated with IGF1 and IGF2 proteins to gain insights into the role of these two IGF ligands in the regulation of ovarian development and maturation. A total of 1494 lncRNAs and 8728 mRNAs were differentially expressed following IGF1 treatm

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Chen, Ray-Jade, His-Chin Wu, Mu-Hsin Chang та ін. "Leu27IGF2 plays an opposite role to IGF1 to induce H9c2 cardiomyoblast cell apoptosis via Gαq signaling". Journal of Molecular Endocrinology 43, № 6 (2009): 221–30. http://dx.doi.org/10.1677/jme-08-0121.

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This study examines the role of IGF2/mannose 6-phosphate receptor (IGF2R) signaling in the signaling transduction regulation and cell apoptosis in H9c2 cardiomyoblast cells. However, it is difficult to recognize the distinct activation of IGF2 signaling without interfacing with IGFI receptor (IGF1R) after exposure to IGF2. Leu27IGF2, an analog of IGF2 that interacts selectively with the IGF2R, was used to specifically activate IGF2R signaling in this study. DNA fragmentation and TUNEL assay revealed that in contrast to IGF1 treatment preventing angiotensin II and AG1024-induced cell apoptosis,

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Bustamante-Marin, Ximena, Kaylyn L. Devlin, Shannon B. McDonell, et al. "Regulation of IGF1R by MicroRNA-15b Contributes to the Anticancer Effects of Calorie Restriction in a Murine C3-TAg Model of Triple-Negative Breast Cancer." Cancers 15, no. 17 (2023): 4320. http://dx.doi.org/10.3390/cancers15174320.

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Calorie restriction (CR) inhibits triple-negative breast cancer (TNBC) progression in several preclinical models in association with decreased insulin-like growth factor 1 (IGF1) signaling. To investigate the impact of CR on microRNAs (miRs) that target the IGF1/IGF1R pathway, we used the spontaneous murine model of TNBC, C3(1)/SV40 T-antigen (C3-TAg). In C3-TAg mice, CR reduced body weight, IGF1 levels, and TNBC progression. We evaluated the tumoral expression of 10 miRs. CR increased the expression of miR-199a-3p, miR-199a-5p, miR-486, and miR-15b. However, only miR-15b expression correlated

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Klich, Izabela, Maciek Tarnowski, Dong-Myung Shin, Janina Ratajczak, Magdalena Kucia, and Mariusz Z. Ratajczak. "Quiescent Status of Very Small Embryonic Like Stem Cells (VSELs) Points to Pivotal Role of Autocrine Role of Insulin-Like Growth Factor-2 (Igf2) – Ras-Activating Guanine Nucleotide Exchange Factor (Rasgrf1) Axis in Regulating Proliferation of Embryonic Stem Cells." Blood 114, no. 22 (2009): 1484. http://dx.doi.org/10.1182/blood.v114.22.1484.1484.

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Abstract Abstract 1484 Poster Board I-507 The proper balance in expression of so-called imprinted genes is crucial for initiation of embryogenesis. Overall, there are ∼80 imprinted genes (expressed either from maternal or paternal chromosomes only), for which proper mono-allelic expression regulates totipotential status of the zygote and pluripotency of developmentally early stem cells. Of these 80 genes, only three (Igf2-H19, Rasgrf1, Meg3) are paternally methylated. We reported that the population of pluripotent VSELs deposited in adult organs (Leukemia 2006:20;857) is kept in quiescent stat

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Moody, Gordon, Pedro J. Beltran, Petia Mitchell, et al. "IGF1R blockade with ganitumab results in systemic effects on the GH–IGF axis in mice." Journal of Endocrinology 221, no. 1 (2014): 145–55. http://dx.doi.org/10.1530/joe-13-0306.

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Ganitumab is a fully human MAB to the human type 1 IGF receptor (IGF1R). Binding assays showed that ganitumab recognized murine IGF1R with sub-nanomolar affinity (KD=0.22 nM) and inhibited the interaction of murine IGF1R with IGF1 and IGF2. Ganitumab inhibited IGF1-induced activation of IGF1R in murine lungs and CT26 murine colon carcinoma cells and tumors. Addition of ganitumab to 5-fluorouracil resulted in enhanced inhibition of tumor growth in the CT26 model. Pharmacological intervention with ganitumab in naïve nude mice resulted in a number of physiological changes described previously in

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Rosenzweig, Steven A. "The Continuing Evolution of Insulin-like Growth Factor Signaling." F1000Research 9 (March 23, 2020): 205. http://dx.doi.org/10.12688/f1000research.22198.1.

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The insulin-like growth factors (IGFs; IGF1/IGF2), known for their regulation of cell and organismal growth and development, are evolutionarily conserved ligands with equivalent peptides present in flies (D. melanogaster), worms (C. elegans) among others. Two receptor tyrosine kinases, the IGF1 receptor and the insulin receptor mediate the actions of these ligands with a family of IGF binding proteins serving as selective inhibitors of IGF1/2. This treatise reviews recent findings on IGF signaling in cancer biology and central nervous system function. This includes overexpression of IGF1 recep

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Fujita, Masaaki, Katsuaki Ieguchi, Parastoo Davari та ін. "Cross-talk between Integrin α6β4 and Insulin-like Growth Factor-1 Receptor (IGF1R) through Direct α6β4 Binding to IGF1 and Subsequent α6β4-IGF1-IGF1R Ternary Complex Formation in Anchorage-independent Conditions". Journal of Biological Chemistry 287, № 15 (2012): 12491–500. http://dx.doi.org/10.1074/jbc.m111.304170.

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Integrin αvβ3 plays a role in insulin-like growth factor-1 (IGF1) signaling (integrin-IGF1 receptor (IGF1R) cross-talk). The specifics of the cross-talk are, however, unclear. In a current model, “ligand occupancy” of αvβ3 (i.e. the binding of extracellular matrix proteins) enhances signaling induced by IGF1 binding to IGF1R. We recently reported that IGF1 directly binds to αvβ3 and induces αvβ3-IGF1-IGF1R ternary complex formation. Consistently, the integrin binding-defective IGF1 mutant (R36E/R37E) is defective in inducing ternary complex formation and IGF signaling, but it still binds to IG

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Rockstroh, Denise, Heike Pfäffle, Diana Le Duc, et al. "A new p.(Ile66Serfs*93) IGF2 variant is associated with pre- and postnatal growth retardation." European Journal of Endocrinology 180, no. 1 (2019): K1—K13. http://dx.doi.org/10.1530/eje-18-0601.

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Objective The IGF/IGF1R axis is involved in the regulation of human growth. Both IGF1 and IGF2 can bind to the IGF1R in order to promote growth via the downstream PI3K/AKT pathway. Pathogenic mutations in IGF1 and IGF1R determine intrauterine growth restriction and affect postnatal body growth. However, to date, there are only few reports of pathogenic IGF2 mutations causing severe prenatal, as well as postnatal growth retardation. Results Here we describe a de novo c.195delC IGF2 variant (NM_000612, p.(Ile66Serfs*93)) in a 4-year-old patient with severe pre- and post-natal growth retardation

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Kineman, Rhonda D., Mercedes del Rio-Moreno, and André Sarmento-Cabral. "40 YEARS of IGF1: Understanding the tissue-specific roles of IGF1/IGF1R in regulating metabolism using the Cre/loxP system." Journal of Molecular Endocrinology 61, no. 1 (2018): T187—T198. http://dx.doi.org/10.1530/jme-18-0076.

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It is clear that insulin-like growth factor-1 (IGF1) is important in supporting growth and regulating metabolism. The IGF1 found in the circulation is primarily produced by the liver hepatocytes, but healthy mature hepatocytes do not express appreciable levels of the IGF1 receptor (IGF1R). Therefore, the metabolic actions of IGF1 are thought to be mediated via extra-hepatocyte actions. Given the structural and functional homology between IGF1/IGF1R and insulin receptor (INSR) signaling, and the fact that IGF1, IGF1R and INSR are expressed in most tissues of the body, it is difficult to separat

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Halmos, Tamás, and Ilona Suba. "A növekedési hormon és az inzulinszerű növekedési faktorok élettani szerepe." Orvosi Hetilap 160, no. 45 (2019): 1774–83. http://dx.doi.org/10.1556/650.2019.31507.

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Abstract: The existence of insulin-like growth factors (IGFs) was recognized in connection with the stimulation of sulfate incorporation into cartilage. IGFs take part in the embryonal development and postnatal growth, in interaction with the growth hormone (GH). The physiological effects of IGF1 are promotion of tissue growth and development, stimulation of cell proliferation, effects on lipid and carbohydrate metabolism, anti-aging, anti-inflammatory, anabolic, anti-oxidant, neuro- and hepatoprotective properties. Our knowledge about the GH/IGF axis is diverse, partly contradictory, their re

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Bäck, Karolina, Rakibul Islam, Git S. Johansson, Simona I. Chisalita, and Hans J. Arnqvist. "Insulin and IGF1 receptors in human cardiac microvascular endothelial cells: metabolic, mitogenic and anti-inflammatory effects." Journal of Endocrinology 215, no. 1 (2012): 89–96. http://dx.doi.org/10.1530/joe-12-0261.

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Diabetes is associated with microcirculatory dysfunction and heart failure and changes in insulin and IGF1 levels. Whether human cardiac microvascular endothelial cells (HMVEC-Cs) are sensitive to insulin and/or IGF1 is not known. We studied the role of insulin receptors (IRs) and IGF1 receptors (IGF1Rs) in metabolic, mitogenic and anti-inflammatory responses to insulin and IGF1 in HMVEC-Cs and human umbilical vein endothelial cells (HUVECs). IR and IGF1R gene expression was studied using real-time RT-PCR. Receptor protein expression and phosphorylation were determined by western blot and ELIS

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Pierce, A. L., J. T. Dickey, L. Felli, P. Swanson, and W. W. Dickhoff. "Metabolic hormones regulate basal and growth hormone-dependent igf2 mRNA level in primary cultured coho salmon hepatocytes: effects of insulin, glucagon, dexamethasone, and triiodothyronine." Journal of Endocrinology 204, no. 3 (2009): 331–39. http://dx.doi.org/10.1677/joe-09-0338.

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Igf1 and Igf2 stimulate growth and development of vertebrates. Circulating Igfs are produced by the liver. In mammals, Igf1 mediates the postnatal growth-promoting effects of growth hormone (Gh), whereas Igf2 stimulates fetal and placental growth. Hepatic Igf2 production is not regulated by Gh in mammals. Little is known about the regulation of hepatic Igf2 production in nonmammalian vertebrates. We examined the regulation of igf2 mRNA level by metabolic hormones in primary cultured coho salmon hepatocytes. Gh, insulin, the glucocorticoid agonist dexamethasone (Dex), and glucagon increased igf

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Ashraf Soliman, Noor Hamed, Fawzia Alyafei, et al. "Insulin like growth factor 1 (IGF-1) and IGF binding proteins in obese pregnant women and their babies: Potential effects on placental function and fetal growth." World Journal of Advanced Research and Reviews 17, no. 2 (2023): 093–100. http://dx.doi.org/10.30574/wjarr.2023.17.2.0222.

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Introduction: The placenta expresses significant amounts of insulin and IGF1 receptors at distinct locations on both fetal and maternal surfaces. This makes the IGF1 and the insulin receptor accessible to fetal and/or maternal insulin, IGF1 and IGF2. IGFs are involved in the receptor-mediated regulation of placental growth and transport, and placental angiogenesis. Maternal obesity during gestation mediates significant changes in the metabolism of mothers, placentas as well as fetal growth. Objectives: In obese women. the role of the insulin like growth factor system IGFs, IGF receptors, IGF-b

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Ashraf, Soliman, Hamed Noor, Alyafei Fawzia, et al. "Insulin like growth factor 1 (IGF-1) and IGF binding proteins in obese pregnant women and their babies: Potential effects on placental function and fetal growth." World Journal of Advanced Research and Reviews 17, no. 2 (2023): 093–100. https://doi.org/10.5281/zenodo.8093130.

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<strong>Introduction</strong>: The placenta expresses significant amounts of insulin and IGF1 receptors at distinct locations on both fetal and maternal surfaces. This makes the IGF1 and the insulin receptor accessible to fetal and/or maternal insulin, IGF1 and IGF2. IGFs are involved in the receptor-mediated regulation of placental growth and transport, and placental angiogenesis. Maternal obesity during gestation mediates significant changes in the metabolism of mothers, placentas as well as fetal growth. <strong>Objectives</strong>: In obese women. the role of the insulin like growth factor

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Archilia, Evandro C., Camilo Andres P. Bello, Isadora M. Batalha, and Luis F. Schütz. "PSXII-23 Sirtuins expression and activity in bovine granulosa cells are regulated by hormones that influence ovarian steroidogenesis." Journal of Animal Science 99, Supplement_3 (2021): 424–25. http://dx.doi.org/10.1093/jas/skab235.762.

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Abstract Sirtuins (SIRTs) are a family of seven NAD+-dependent histone deacetylases that regulate several biological reactions. How SIRTs regulate ovarian steroidogenesis in cattle remains to be fully unveiled. We hypothesize that SIRTs expression and activity are regulated by hormones that influence steroidogenesis in bovine granulosa cells (GC). Bovine ovaries were collected at an USDA-inspected commercial slaughterhouse and GC were isolated from small antral follicles (1–5 mm on surface diameter). Cells were treated with hormones that regulate ovarian folliculogenesis: FSH, IGF1, fibroblast

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Li, Jianzhen, Zhiquan Liu, Tao Kang, Minghui Li, Deshou Wang, and Christopher H. K. Cheng. "Igf3: a novel player in fish reproduction." Biology of Reproduction 104, no. 6 (2021): 1194–204. http://dx.doi.org/10.1093/biolre/ioab042.

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Abstract As in other vertebrates, fish reproduction is tightly controlled by gonadotropin signaling. One of the most perplexing aspects of gonadotropin action on germ cell biology is the restricted expression of gonadotropin receptors in somatic cells of the gonads. Therefore, the identification of factors conveying the action of gonadotropins on germ cells is particularly important for understanding the mechanism of reproduction. Insulin-like growth factors (Igfs) are recognized as key factors in regulating reproduction by triggering a series of physiological processes in vertebrates. Recentl

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Keselman, Ana Claudia, Ayelen Martin, Paula Alejandra Scaglia, et al. "A homozygous mutation in the highly conserved Tyr60 of the mature IGF1 peptide broadens the spectrum of IGF1 deficiency." European Journal of Endocrinology 181, no. 5 (2019): K43—K53. http://dx.doi.org/10.1530/eje-19-0563.

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Background IGF1 is a key factor in fetal and postnatal growth. To date, only three homozygous IGF1 gene defects leading to complete or partial loss of IGF1 activity have been reported in three short patients born small for gestational age. We describe the fourth patient with severe short stature presenting a novel homozygous IGF1 gene mutation. Results We report a boy born from consanguineous parents at 40 weeks of gestational age with intrauterine growth restriction and severe postnatal growth failure. Physical examination revealed proportionate short stature, microcephaly, facial dysmorphism

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Schwiebert, Christian, Peter Kühnen, Niels-Peter Becker, et al. "Antagonistic Autoantibodies to Insulin-Like Growth Factor-1 Receptor Associate with Poor Physical Strength." International Journal of Molecular Sciences 21, no. 2 (2020): 463. http://dx.doi.org/10.3390/ijms21020463.

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Natural autoantibodies to the IGF1 receptor (IGF1R-aAb) have been described in relation to Graves’ ophthalmopathy. Other physiological roles of natural IGF1R-aAb are not known. We hypothesized that IGF1R-aAb may be related to muscle development. Serum samples (n = 408) from young overweight subjects (n = 143) were collected during a lifestyle intervention study. Anthropometric parameters, along with leptin, IGF1 and IGF1R-aAb concentrations, were analyzed, and the subjects were categorized into positive or negative for IGF1R-aAb. Eleven out of 143 subjects (7.7%) were positive for IGF1R-aAb. I

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Lucy, Matthew C. "Growth hormone regulation of follicular growth." Reproduction, Fertility and Development 24, no. 1 (2012): 19. http://dx.doi.org/10.1071/rd11903.

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The somatotropic axis – consisting of growth hormone (GH), the insulin-like growth factors 1 and 2 (IGF1 and IGF2), GH binding protein (GHBP), IGF binding proteins (IGFBPs) 1 to 6, and the cell-surface receptors for GH and the IGFs – has major effects on growth, lactation and reproduction. The primary target tissues for GH are involved in growth and metabolism. The functionality of the somatotropic axis depends in part on the expression of liver GH receptor (GHR), which determines the amount of IGF1 released from the liver in response to GH. The IGF1 acts as a pleiotropic growth factor and als

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Zhao, Chunyan, Sujie Zheng, Yongji Dang, Mengshu Wang, and Yichao Ren. "Identification of a New Insulin-like Growth Factor 3 (igf3) in Turbot (Scophthalmus maximus): Comparison and Expression Analysis of IGF System Genes during Gonadal Development." Fishes 8, no. 5 (2023): 240. http://dx.doi.org/10.3390/fishes8050240.

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Fish reproduction is closely related to the regulation of the brain and liver, making it essential to identify the factors that control this process. The turbot (Scophthalmus maximus) is an economically significant species that has been successfully breeding through industrial aquaculture. Investigation of factors into the involvement of gonadal development is crucial for artificial breeding. In this study, a new insulin-like growth factor 3 igf3 gene was cloned and characterized. Additionally, all three types of turbot IGFs contain a distinct IGF domain, with IGF3 and IGF2 being grouped with

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Varewijck, A. J., A. J. van der Lely, S. J. C. M. M. Neggers, S. W. J. Lamberts, L. J. Hofland, and J. A. M. J. L. Janssen. "In active acromegaly, IGF1 bioactivity is related to soluble Klotho levels and quality of life." Endocrine Connections 3, no. 2 (2014): 85–92. http://dx.doi.org/10.1530/ec-14-0028.

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The value of measuring IGF1 bioactivity in active acromegaly is unknown. Soluble Klotho (S-Klotho) level is elevated in active acromegaly and it has been suggested that S-Klotho can inhibit activation of the IGF1 receptor (IGF1R). A cross-sectional study was carried out in 15 patients with active acromegaly based on clinical presentation, unsuppressed GH during an oral glucose tolerance test, and elevated total IGF1 levels (>+2 s.d.). Total IGF1 was measured by immunoassay, IGF1 bioactivity by the IGF1R kinase receptor activation assay and S-Klotho by an ELISA. Quality of Life (QoL) was ass

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Fowke, Jay H., Charles E. Matthews, Herbert Yu, et al. "Racial differences in the association between body mass index and serum IGF1, IGF2, and IGFBP3." Endocrine-Related Cancer 17, no. 1 (2010): 51–60. http://dx.doi.org/10.1677/erc-09-0023.

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African–American (AA) race/ethnicity, lower body mass index (BMI), and higher IGF1 levels are associated with premenopausal breast cancer risk. This cross-sectional analysis investigated whether BMI or BMI at age 21 years contributes to racial differences in IGF1, IGF2, IGF-binding protein 3 (IGFBP3), or free IGF1. Participants included 816 white and 821 AA women between ages 40 and 79 years across a wide BMI range (18.5–40 kg/m2). Compared with white women, AA women had higher mean IGF1 (146.3 vs 134.4 ng/ml) and free IGF1 (0.145 vs 0.127) levels, and lower IGF2 (1633.0 vs 1769.3 ng/ml) and I

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Das, Debabrata, Soumojit Pal, and Sudipta Maitra. "Releasing prophase arrest in zebrafish oocyte: synergism between maturational steroid and Igf1." REPRODUCTION 151, no. 1 (2016): 59–72. http://dx.doi.org/10.1530/rep-15-0389.

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Binding of 17β-estradiol (E2) to novel G-protein coupled receptor, Gper1, promotes intra-oocyte adenylyl cyclase activity and transactivates epidermal growth factor receptor to ensure prophase-I arrest. Although involvement of either membrane progestin receptor (mPR) or Igf system has been implicated in regulation of meiosis resumption, possibility of concurrent activation and potential synergism between 17α,20β-dihydroxy-4-pregnen-3-one (DHP)- and Igf-mediated signalling cascades in alleviating E2 inhibition of oocyte maturation (OM) has not been investigated. Here using zebrafish (Danio reri

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Espelund, Ulrick, Søren Cold, Jan Frystyk, Hans Ørskov, and Allan Flyvbjerg. "Elevated free IGF2 levels in localized, early-stage breast cancer in women." European Journal of Endocrinology 159, no. 5 (2008): 595–601. http://dx.doi.org/10.1530/eje-08-0154.

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ObjectiveEpidemiological studies imply an association between circulating IGF1 and breast cancer, whereas the role of IGF2, which also acts on the IGF1 receptor, is less settled. This study investigates the association between IGF2 and breast cancer in patients with localized disease.DesignThe participants were women with well-characterized, early stage, localized breast cancer (n=43) and matched healthy women (n=38), from whom fasting serum levels of IGF-related peptides were measured.ResultsIn patients, mean free IGF2 was increased (+57%, P<0.001), in spite of reduced total IGF2 levels (−

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Al-Hassani, Ali S. "BROILER GENOTYPING AND ALLILIC FREQUNCY ANALYSIS BY PCR-RFLP FOR IGF1 AND IGF2 GENES." IRAQI JOURNAL OF AGRICULTURAL SCIENCES 53, no. 5 (2022): 1235–40. http://dx.doi.org/10.36103/ijas.v53i5.1637.

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This study was aimed to investigate Broiler chickens using PCR-RFLP (IGF1 and IGF2 genes). HinfI and Hsp92II restriction enzymes were used to amplify and digest 793-bp and 1146-bp fragments of the IGF1 gene, respectively. TT, TC, and CC genotypes were found in both genes in two different T and C allele subgroups. IGF1 had a higher prevalence of the C-allele (0.86) than IGF2, whereas IGF2 had a higher prevalence of the T-allele (0.5611).

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Caroni, P., and P. Grandes. "Nerve sprouting in innervated adult skeletal muscle induced by exposure to elevated levels of insulin-like growth factors." Journal of Cell Biology 110, no. 4 (1990): 1307–17. http://dx.doi.org/10.1083/jcb.110.4.1307.

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Partial denervation or paralysis of adult skeletal muscle is followed by nerve sprouting, probably due to release of diffusible sprout-inducing activity by inactive muscle. Insulin-like growth factors (IGF1 and IFG2) are candidates for muscle-derived sprouting activity, because (a) they induce neurite growth from peripheral neurons in vitro; and (b) their mRNA levels in adult skeletal muscle increase severalfold after denervation or paralysis. We sought to determine whether the presence of elevated levels of IGFs in innervated adult skeletal muscle was sufficient to produce intramuscular nerve

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Clemmons, David R. "40 YEARS OF IGF1: Role of IGF-binding proteins in regulating IGF responses to changes in metabolism." Journal of Molecular Endocrinology 61, no. 1 (2018): T139—T169. http://dx.doi.org/10.1530/jme-18-0016.

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The IGF-binding protein family contains six members that share significant structural homology. Their principal function is to regulate the actions of IGF1 and IGF2. These proteins are present in plasma and extracellular fluids and regulate access of both IGF1 and II to the type I IGF receptor. Additionally, they have functions that are independent of their ability to bind IGFs. Each protein is regulated independently of IGF1 and IGF2, and this provides an important mechanism by which other hormones and physiologic variables can regulate IGF actions indirectly. Several members of the family ar

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He, Bo, Yinxian Wen, Shuwei Hu, et al. "Prenatal caffeine exposure induces liver developmental dysfunction in offspring rats." Journal of Endocrinology 242, no. 3 (2019): 211–26. http://dx.doi.org/10.1530/joe-19-0066.

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We previously showed that prenatal caffeine exposure (PCE) induces intrauterine growth retardation (IUGR) and high susceptibility to nonalcoholic fatty liver disease in offspring rats, and the underlying mechanisms are associated with fetal overexposure to maternal glucocorticoids. Herein, we aimed to verify whether PCE disrupts liver development before and after birth and explore its possible programming mechanism. In vivo, reduced fetal weights and increased IUGR rates were accompanied by fetal liver developmental dysfunction in PCE rats. Increased fetal serum corticosterone and decreased in

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Durfort, Tiphanie, Luca Mazzarella, and Pier Giuseppe Pelicci. "Insulin/IGF1 Pathway Inhibition in Combination with All-Trans Retinoic Acid in Acute Promyelocytic Leukemia." Blood 120, no. 21 (2012): 1347. http://dx.doi.org/10.1182/blood.v120.21.1347.1347.

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Abstract Abstract 1347 Chemotherapy-free regimens are now a realistic goal in Acute Promyelocytic Leukemia (APL), as demonstrated by recent studies combining All-Trans Retinoic Acid (ATRA) with Arsenic Trioxide and Gemtuzumab Ozogamicin. Combinations of ATRA with kinase inhibitors, which have shown tremendous efficacy in several malignancies, are interesting alternatives but remain poorly characterized in APL. The Insulin-like growth factor 1 (IGF1) pathway is an attractive drug target because of its pervasive involvement in cell proliferation and metabolism of cancer cells. A variety of strat

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Ding, Ning, Dehong Tian, Xue Li, et al. "Genetic Polymorphisms of IGF1 and IGF1R Genes and Their Effects on Growth Traits in Hulun Buir Sheep." Genes 13, no. 4 (2022): 666. http://dx.doi.org/10.3390/genes13040666.

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The identification of candidate genes and genetic variations associated with growth traits is important for sheep breeding. Insulin like growth factor 1 (IGF1) and insulin like growth factor 1 receptor (IGF1R) are well-accepted candidate genes that affect animal growth and development. The current study attempted to assess the association between IGF1 and IGF1R genetic polymorphisms and growth traits in Hulun Buir sheep. To achieve this goal, we first identified three and ten single nucleotide polymorphisms (SNPs) in exons of IGF1 and IGF1R in Hulun Buir sheep and then constructed six haplotyp

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Meyer, K. F., S. Krauss-Etschmann, W. Kooistra, et al. "Prenatal exposure to tobacco smoke sex dependently influences methylation and mRNA levels of the Igf axis in lungs of mouse offspring." American Journal of Physiology-Lung Cellular and Molecular Physiology 312, no. 4 (2017): L542—L555. http://dx.doi.org/10.1152/ajplung.00271.2016.

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Prenatal smoke exposure is a risk factor for abnormal lung development and increased sex-dependent susceptibility for asthma and chronic obstructive pulmonary disease (COPD). Birth cohort studies show genome-wide DNA methylation changes in children from smoking mothers, but evidence for sex-dependent smoke-induced effects is limited. The insulin-like growth factor (IGF) system plays an important role in lung development. We hypothesized that prenatal exposure to smoke induces lasting changes in promoter methylation patterns of Igf1 and Igf1r, thus influencing transcriptional activity and contr

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Teissier, R., I. Flechtner, A. Colmenares, et al. "Characterization and prevalence of severe primary IGF1 deficiency in a large cohort of French children with short stature." European Journal of Endocrinology 170, no. 6 (2014): 847–54. http://dx.doi.org/10.1530/eje-14-0071.

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ObjectiveThe prevalence of severe primary IGF1 deficiency (IGFD) is unclear. IGFD must be identified promptly as treatment with recombinant human IGF1 (rhIGF1) is now available. Our objective was to characterize and assess the prevalence of severe primary IGFD in a large cohort of patients evaluated for short stature at a pediatric endocrinology unit in France.DesignObservational study in a prospective cohort.MethodsConsecutive patients referred to our unit between 2004 and 2009 for suspected slow statural growth were included. Patients were classified into eight etiological categories. IGFD w

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Melhem, Molly R., Aldo M. Roccaro, Mena Farag, et al. "The Effect of Insulin-Like Growth Factor 1 on Waldenstrom Macroglobulinemia." Blood 112, no. 11 (2008): 4994. http://dx.doi.org/10.1182/blood.v112.11.4994.4994.

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Abstract Background: Waldenstrom’s Macroglobulinemia (WM) is an incurable lymphoplasmacytic lymphoma with limited options of therapy. Insulin-like growth factor 1 (IGF1) is a polypeptide hormone that has been shown to have a proactive role in many cancer cell types, including multiple myeloma and solid tumor cells. We evaluated the role of IGF1 in WM. Methods: WM cell lines (BCWM1 and WSU-WM) and IgM secreting low-grade lymphoma cell lines (MEC1, RL) were used. Bone marrow-derived primary CD19+ cells and bone marrow stromal cells (BMSC) were obtained from patients with WM after informed consen

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Tanaka, Yuko, Seiichi Okabe, Tetsuzo Tauchi, Yoshikazu Ito, and Kazuma Ohyashiki. "Targeting Insulin-like Growth Factor in Multiple Myeloma: Novel Strategies in the Treatment of Proteasome Inhibitor Resistant Cells." Blood 132, Supplement 1 (2018): 5155. http://dx.doi.org/10.1182/blood-2018-99-114750.

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Abstract Introduction: Multiple myeloma (MM) is one of the hematological malignancy that affects the plasma cells in bone marrow. The treatment of MM patients has been dramatically changed by new agents such as proteasome inhibitors and immunomodulatory drugs. However, most patients will relapse even if new agents provide therapeutic advantages. Therefore, a new strategy is still needed to increase MM patient survival. Insulin-like growth factor (IGF) cause intracellular signaling that ultimately results in cellular growth and proliferation. Because IGF signaling pathways have crucial function

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Picha, Matthew E., Marc J. Turano, Christian K. Tipsmark, and Russell J. Borski. "Regulation of endocrine and paracrine sources of Igfs and Gh receptor during compensatory growth in hybrid striped bass (Morone chrysops×Morone saxatilis)." Journal of Endocrinology 199, no. 1 (2008): 81–94. http://dx.doi.org/10.1677/joe-07-0649.

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Compensatory growth (CG) is a period of growth acceleration that exceeds normal rates after animals are alleviated of certain growth-stunting conditions. In hybrid striped bass (HSB, Morone chrysops×Morone saxatilis), 3 weeks of complete feed restriction results in a catabolic state that, when relieved, renders a subsequent phase of CG. The catabolic state was characterized by depressed levels of hepatic Type I and II GH receptor (ghr1, ghr2) and igf1 mRNA, along with considerable decreases in plasma Igf1. The state of catabolism also resulted in significant declines in hepatic igf2 mRNA and i

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Birzniece, Vita, Nils Erik Magnusson, Ken K. Y. Ho, and Jan Frystyk. "Effects of raloxifene and estrogen on bioactive IGF1 in GH-deficient women." European Journal of Endocrinology 170, no. 3 (2014): 375–83. http://dx.doi.org/10.1530/eje-13-0835.

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ContextGH action is attenuated by estrogens and selective estrogen receptor modulators (SERMs) administered orally. During GH therapy in hypopituitary women, co-treatment with raloxifene, a SERM, induced a smaller gain in lean body mass (LBM) compared with estrogen, despite an equal reduction in IGF1. As a higher IGF-binding protein-3 (IGFBP3) level was observed with raloxifene co-treatment, we hypothesize that an increase in IGFBP3 reduced IGF1 bioactivity causing the attenuated anabolic effect.ObjectiveTo assess the effects of 17β-estradiol (E2) and raloxifene on bioactive IGF1.DesignIn stud

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Erdem, Cemal, Adrian V. Lee, D. Lansing Taylor, and Timothy R. Lezon. "Inhibition of RPS6K reveals context-dependent Akt activity in luminal breast cancer cells." PLOS Computational Biology 17, no. 6 (2021): e1009125. http://dx.doi.org/10.1371/journal.pcbi.1009125.

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Aberrant signaling through insulin (Ins) and insulin-like growth factor I (IGF1) receptors contribute to the risk and advancement of many cancer types by activating cell survival cascades. Similarities between these pathways have thus far prevented the development of pharmacological interventions that specifically target either Ins or IGF1 signaling. To identify differences in early Ins and IGF1 signaling mechanisms, we developed a dual receptor (IGF1R & InsR) computational response model. The model suggested that ribosomal protein S6 kinase (RPS6K) plays a critical role in regulating MAPK

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