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Journal articles on the topic 'IgG levels'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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1

Anam, Khairul, Farhat Afrin, Dwijadas Banerjee, et al. "Immunoglobulin Subclass Distribution and Diagnostic Value of Leishmania donovani Antigen-Specific Immunoglobulin G3 in Indian Kala-Azar Patients." Clinical Diagnostic Laboratory Immunology 6, no. 2 (1999): 231–35. http://dx.doi.org/10.1128/cdli.6.2.231-235.1999.

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ABSTRACT Visceral leishmaniasis, or kala-azar, a fatal tropical disease, remains problematic, as early diagnosis is difficult and treatment often results in drug resistance and relapse. We have developed a sensitive enzyme-linked immunosorbent assay (ELISA), using leishmanial membrane antigenic extracts (LAg) to detect specific antibody responses in 25 untreated Indian visceral leishmaniasis patients. To investigate the pathogenetic significance of isotype markers in kala-azar, relative levels of specific immunoglobulin G (IgG), IgM, IgA, IgE, and IgG subclasses were analyzed under clinically

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Anam, Khairul, Farhat Afrin, Dwijadas Banerjee, et al. "Differential Decline in Leishmania Membrane Antigen-Specific Immunoglobulin G (IgG), IgM, IgE, and IgG Subclass Antibodies in Indian Kala-Azar Patients after Chemotherapy." Infection and Immunity 67, no. 12 (1999): 6663–69. http://dx.doi.org/10.1128/iai.67.12.6663-6669.1999.

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ABSTRACT Pathogenesis in kala-azar is associated with depressed cellular immunity and significant elevation of antileishmanial antibodies. Since these antibodies are present even after cure, analysis of the parasite-specific isotypes and immunoglobulin G (IgG) subclasses in kala-azar patients may shed new light on the immune responses during progression and resolution of infection. Using leishmanial membrane antigenic extracts, we investigated the relative levels of specific IgG, IgM, IgA, IgE, and IgG subclasses in Indian kala-azar patient sera during disease, drug resistance, and cure. Acute

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Ramezanpour, Mahnaz, Hua Hu, Aden Lau, et al. "Increased Serum IgG4 Associates with Asthma and Tissue Eosinophilia in Chronic Rhinosinusitis Patients." Pathogens 9, no. 10 (2020): 828. http://dx.doi.org/10.3390/pathogens9100828.

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Chronic Rhinosinusitis (CRS) is a multifactorial disease where microorganisms’ innate and adaptive immunity can play a role. This study assessed the total IgG, IgG subclasses, IgE and IgA levels in serum samples from CRS and non-CRS control patients in relation to the disease severity, phenotype, histopathology and comorbidities. Total serum IgG, IgG1, IgG2, IgG3, IgG4 and IgE was determined from 10 non-CRS controls, 10 CRS without nasal polyp (CRSsNP) and 26 CRS with nasal polyp (CRSwNP) patients using ImmunoCap assays. Tissue lysates were analyzed for IgG levels by ELISA. Immunohistochemical

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Weetman, A. P., and Shara B. Cohen. "The relationship of HLA-DR3 and outcome after antithyroid drugs to the IgG subclass distribution of thyroid autoantibodies in Graves' disease." Acta Endocrinologica 114, no. 2 (1987): 292–97. http://dx.doi.org/10.1530/acta.0.1140292.

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Abstract. We have examined the IgG subclass distribution of thyroglobulin (Tg) and microsomal (M) auto-antibodies in 31 patients with Graves' disease before and after treatment with carbimazole. IgG1 and IgG2 subclass antibodies were detected before treatment in nearly all patients, and in over a third there was an excess of activity in the IgG3 and IgG4 subclasses. There were significant differences between the IgG subclasses composing Tg antibodies (less IgG 1 and IgG3 but more IgG2 in the latter). Patients who were HLA-DR3-positive had significantly lower levels of IgG4 in M antibodies than

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Tappe, Dennis, Yasuhito Sako, Sonoyo Itoh, et al. "Immunoglobulin G Subclass Responses to Recombinant Em18 in the Follow-Up of Patients with Alveolar Echinococcosis in Different Clinical Stages." Clinical and Vaccine Immunology 17, no. 6 (2010): 944–48. http://dx.doi.org/10.1128/cvi.00026-10.

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ABSTRACT In this study, we compared the sequential responses of immunoglobulin G (IgG) subclasses to the diagnostic antigen Em18 in sera from patients with alveolar echinococcosis. A total of 225 sera from 36 patients at different clinical stages according to the WHO-PNM staging system were tested. The antibody responses were measured for cohorts with resected and unresected parasitic lesions by enzyme-linked immunosorbent assays (ELISA). Total IgG and, to a lesser extent, IgG4 antibody levels against Em18 correlated with all PNM stages before treatment, whereas levels of IgG2 were low and IgG

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McDonald, Vickie, Samuel J. Machin, Ian J. Mackie, and Marie A. Scully. "The Prognostic Effects of Anti-ADAMTS13 IgG, IgA and IgM Antibody Subclasses In Acute Idiopathic TTP and the Effect of Rituximab,." Blood 118, no. 21 (2011): 3305. http://dx.doi.org/10.1182/blood.v118.21.3305.3305.

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Abstract Abstract 3305 Acquired idiopathic TTP is associated with severe ADAMTS13 (A13) deficiency/anti-A13 IgG antibodies. Management is with plasma exchange (PEX) and immunosuppression. Rituximab is known to induce a sustained remission by eradicating the A13 inhibitor. We studied the prognostic value of anti-A13 IgG subclasses and the effect of rituximab on these antibodies. Anti-A13 IgG subclasses were detected using ELISA: microtitre plates coated with rADAMTS13 and antibody detected using biotinylated monoclonal anti-IgG1-4. Results: 70 acute episodes from a 4 year period were analysed.

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Aucouturier, P., A. Barra, L. Intrator, et al. "Long lasting IgG subclass and antibacterial polysaccharide antibody deficiency after allogeneic bone marrow transplantation." Blood 70, no. 3 (1987): 779–85. http://dx.doi.org/10.1182/blood.v70.3.779.779.

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Abstract Serum IgG subclasses were measured by a competitive indirect immunoassay with monoclonal antibodies in 31 leukemic patients before and after bone marrow transplantation. Antibodies to Hemophilus influenzae type b (Hib) capsular polysaccharide were determined in 28 cases. Abnormally low or borderline subclass (mostly IgG2 and IgG4) levels were found late after transplant in 23 infected and noninfected patients. These levels persisted for as long as 25 months, in association with low or borderline IgA levels in 78% of the cases. IgG2, IgG4, and IgA often showed a parallel evolution, whe

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Aucouturier, P., A. Barra, L. Intrator, et al. "Long lasting IgG subclass and antibacterial polysaccharide antibody deficiency after allogeneic bone marrow transplantation." Blood 70, no. 3 (1987): 779–85. http://dx.doi.org/10.1182/blood.v70.3.779.bloodjournal703779.

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Serum IgG subclasses were measured by a competitive indirect immunoassay with monoclonal antibodies in 31 leukemic patients before and after bone marrow transplantation. Antibodies to Hemophilus influenzae type b (Hib) capsular polysaccharide were determined in 28 cases. Abnormally low or borderline subclass (mostly IgG2 and IgG4) levels were found late after transplant in 23 infected and noninfected patients. These levels persisted for as long as 25 months, in association with low or borderline IgA levels in 78% of the cases. IgG2, IgG4, and IgA often showed a parallel evolution, whereas IgG1

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Zeng, Yanli, Yan Zhang, Qinggui Chen, et al. "Distribution of IgG subclass anti-nuclear antibodies (ANAs) in systemic lupus erythematosus." Lupus 30, no. 6 (2021): 901–12. http://dx.doi.org/10.1177/0961203321995242.

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Objectives Our study purpose was to detect the distribution of anti-nuclear antibody (ANA) IgG subclasses in patients with systemic lupus erythematosus (SLE) and to evaluate their influence on the inflammatory process in SLE. Methods We determined the serum levels of ANA IgG subclasses from 70 SLE patients, 25 patients with other autoimmune diseases (OAD), and 25 healthy controls using ELISA. The serum level of total ANA IgG and the avidity of ANA IgG, dsDNA IgG, and dsDNA IgG subclasses were analysed by ELISA. Results The results indicated that levels of four ANA IgG subclasses (IgG1, IgG2, I

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MacGlashan Jr, Donald, Santiago Alvarez‐Arango, and Jody Tversky. "Subclasses of allergen‐specific IgG: Serum IgG2 and IgG3 levels are not predicted by IgG1/IgG4 levels." Clinical & Experimental Allergy 51, no. 8 (2021): 1093–95. http://dx.doi.org/10.1111/cea.13977.

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11

Aucan, Christophe, Yves Traoré, François Tall, et al. "High Immunoglobulin G2 (IgG2) and Low IgG4 Levels Are Associated with Human Resistance to Plasmodium falciparum Malaria." Infection and Immunity 68, no. 3 (2000): 1252–58. http://dx.doi.org/10.1128/iai.68.3.1252-1258.2000.

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ABSTRACT There is accumulating evidence for a role of immunoglobulin G (IgG) in protection against malarial infection and disease. Only IgG1 and IgG3 are considered cytophilic and protective against P. falciparum, whereas IgG2 and IgG4 were thought to be neither and even to block protective mechanisms. However, no clear pattern of association between isotypes and protection has so far emerged. We analyzed the isotypic distribution of the IgG response to conserved epitopes and P. falciparum blood-stage extract in 283 malaria-exposed individuals whose occurrence of infection and malaria attack h

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Nakata, Rie, Takeshi Uehara, Mai Iwaya, et al. "Immunostaining With Immunoglobulin G Subclass Antibody Cocktail for Diagnosis of Type 1 Autoimmune Pancreatitis." International Journal of Surgical Pathology 28, no. 8 (2020): 844–49. http://dx.doi.org/10.1177/1066896920924781.

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Background. Immunoglobulin (Ig) G4-related diseases (RDs) are systemic diseases in which serum IgG4 levels are frequently elevated. They can cause diffuse or focal tumor formation, organ swelling, and tissue thickening in organs infiltrated by IgG4 + plasma cells. The diagnostic criteria for IgG4-RDs include an IgG4/IgG ratio >40%, but counting IgG+ cells can be difficult because of the weakness of IgG staining density. We hypothesized that an antibody cocktail of mixed IgG1, IgG2, IgG3, and IgG4 (AC-IgG) might give immunohistochemistry results comparable with those of IgG in IgG4-RD. Metho

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ESCOBAR-PÉREZ, XIOMARA, ALBERTO J. DORTA-CONTRERAS, MARIA TERESA INTERIÁN-MORALES, ELENA NORIS-GARCÍA, and MARITZA FERRÁ-VALDÉS. "IgG2 immunodeficiency: association to pediatric patients with bacterial meningoencephalitis." Arquivos de Neuro-Psiquiatria 58, no. 1 (2000): 141–45. http://dx.doi.org/10.1590/s0004-282x2000000100021.

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An IgG subclass deficiency is often associated with bacterial infections. We studied four pediatric patients suffering from meningoencephalitis, two of them due to Streptococcus pneumoniae and two due to Haemophilus influenzae type b. Simultaneous diagnostic serum and cerebrospinal fluid samples were taken during income. The four subclasses of IgG and albumin were quantified in both biologic fluids by radial immunodiffusion. Very low levels of seric IgG2 with non detectable cerebrospinal fluid IgG2 were found in the patients. No intrathecal IgG subclass synthesis was found in two patients. One

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Freeman, Jane A., Cecily J. Forsyth, Naomi J. Mackinlay, Ping Han, and Stephen P. Mulligan. "Immunoglobulin G (IgG) Subclasses in Chronic Lymphocytic Leukaemia." Blood 112, no. 11 (2008): 4180. http://dx.doi.org/10.1182/blood.v112.11.4180.4180.

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Abstract Hypogammaglobulinaemia is a common complication of Chronic Lymphocytic Leukaemia (CLL) with an incidence that varies considerably in the reported literature. Immunoglobulin replacement therapy has documented benefit for patients with hypogammaglobulinaemia, CLL and recurrent episodes of infection. Very little data exists on IgG subclasses in CLL. We measured IgG subclasses together with immunoglobulins G, A and M, protein EPG and immunofixation in a cohort of patients with CLL representing a variety of disease stages, treatment status and history of infection to analyse the implicatio

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Shao, Zonghong, Yuanyuan Shao, and Rong Fu. "Study of IgG Subclass of the Patients with Immuno-Related Hemocytopenia." Blood 126, no. 23 (2015): 4604. http://dx.doi.org/10.1182/blood.v126.23.4604.4604.

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Abstract Objective: To investigate the immunoglobulin G (IgG)sublass on the membrane of bone marrow(BM) hematopoietic cells and IgG levels in BM supernatant of patients with immuno-related hemtocytopenia(IRH). Method: Newly diagnosed IRH patients and ICUS patients were enrolled in this study, as well as healthy donors as controls. The categories of auto-antibodies(IgM and IgG) on the membrane of BM hemmatopoietic cells(CD15+,CD34+ and GlyCoA+ cells)were assayed by Flow cytometry(FCM). The categories of auto-antibodies(IgG1 and IgG3) on the membrane of BM hemmatopoietic cells were assayed by FC

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Barton, James C., Luigi F. Bertoli, J. Clayborn Barton, and Ronald T. Acton. "Selective Subnormal IgG1 in 54 Adult Index Patients with Frequent or Severe Bacterial Respiratory Tract Infections." Journal of Immunology Research 2016 (2016): 1–10. http://dx.doi.org/10.1155/2016/1405950.

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We characterized 54 adult index patients with reports of frequent or severe bacterial respiratory tract infections at diagnosis of selective subnormal IgG1. Mean age was50±13(SD) y; 87.0% were women. Associated disorders included the following: autoimmune conditions 50.0%; hypothyroidism 24.1%; atopy 38.9%; and other allergy 31.5%. In 35.5%, proportions of protectiveS. pneumoniaeserotype-specific IgG levels did not increase after polyvalent pneumococcal polysaccharide vaccination (PPPV). Blood lymphocyte subset levels were within reference limits in most patients. Regressions on IgG1 and IgG3

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Ozbılge, Hatice, Nurten Aksoy, Mehmet S. Gurel, and Suleyman Yazar. "IgG and IgG subclass antibodies in patients with active cutaneous leishmaniasis." Journal of Medical Microbiology 55, no. 10 (2006): 1329–31. http://dx.doi.org/10.1099/jmm.0.46667-0.

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This research was planned to detect IgG and IgG subclasses in sera of patients with active cutaneous leishmaniasis (CL). Sera from 30 patients with active CL aged between 10 and 50 years and from 30 healthy controls aged between 8 and 50 years were included in the study. Levels of IgG and its subclasses were measured by a nephelometer. Levels of IgG, IgG1 and IgG3 in the CL patients were higher than in the controls. In addition, IgG and IgG1, and IgG and IgG3 levels showed a significant positive correlation. These results showed that IgG subclasses could possibly be used as a helpful diagnosti

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VENDRAME, Célia Maria V., Márcia Dias T. CARVALHO, Célia Regina F. YAMAMOTO, Maria Cristina NAKHLE, Silvino Alves CARVALHO, and Pedro Paulo CHIEFFI. "Evaluation of anti-Schistosoma mansoni igG antibodies in patients with chronic schistosomiasis mansoni before and after specific treatment." Revista do Instituto de Medicina Tropical de São Paulo 43, no. 3 (2001): 153–59. http://dx.doi.org/10.1590/s0036-46652001000300007.

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The circumoval precipitin test (COPT), enzyme-linked immunosorbent assay (ELISA) and the immunoblotting anti-adult worm antigen (AWA) and soluble egg antigen (SEA) tests were applied to 17 chronically schistosome-infected patients for the detection of anti-Schistosoma mansoni antibodies before and on four occasions after oxamniquine administration over a period of six months. Compared to a control group, schistosomiasis patients showed high levels of IgG antibodies in AWA and SEA-ELISA. A decrease in IgG levels was observed six months after treatment, although negative reactions were not obtai

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Harfi, Harb A., and John T. Godwin. "Normal Serum Levels of IgG, IgA, IgM, IgD, and IgE in Saudi Arabia." Annals of Saudi Medicine 5, no. 2 (1985): 99–104. http://dx.doi.org/10.5144/0256-4947.1985.99.

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Moncunill, Gemma, Alfredo Mayor, Rebeca Santano, et al. "SARS-CoV-2 Seroprevalence and Antibody Kinetics Among Health Care Workers in a Spanish Hospital After 3 Months of Follow-up." Journal of Infectious Diseases 223, no. 1 (2020): 62–71. http://dx.doi.org/10.1093/infdis/jiaa696.

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Abstract Background At the COVID-19 spring 2020 pandemic peak in Spain, prevalence of SARS-CoV-2 infection in a cohort of 578 randomly selected health care workers (HCWs) from Hospital Clínic de Barcelona was 11.2%. Methods A follow-up survey 1 month later (April-May 2020) measured infection by rRT-PCR and IgM, IgA, and IgG to the receptor-binding domain of the spike protein by Luminex. Antibody kinetics, including IgG subclasses, was assessed until month 3. Results At month 1, the prevalence of infection measured by rRT-PCR and serology was 14.9% (84/565) and seroprevalence 14.5% (82/565). We

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Josef, Richter, Kral Vlastimil, Vetvicka Vaclav, Rajnohova Dobiasova Lucie та Fernandez-Botran Rafael. "Effect of β-glucan Supplementation on Levels of IgM, IgA, IgG and Its Subclasses IgG1, IgG2, IgG3, and IgG4 in Cancer Patients". Journal of Tumor 4, № 6 (2016): 469–73. http://dx.doi.org/10.17554/j.issn.1819-6187.2016.04.98.

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Singh, Desh D., L. K. Dwivedi, Sarika Amdekar, and Vinod Singh. "The Immunoglobulin profiling of Schistosoma mansoni infected patients from Central India." South Asian Journal of Experimental Biology 1, no. 5 (2011): 32–35. http://dx.doi.org/10.38150/sajeb.1(5).p32-35.

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Schistosoma mansoni Ã¢â‚¬Âschistosomicimmune response in them. Also, an attempt was made to correlate the observedIg level with the brutality of infection. Consequently, total 138(46.9%) out of 294 volunteers were reported to have ova of Schistosomamansoni in their urine samples. Among which, 84 (28.6%) volunteers with<50 ova in per 10 ml of urine showed light infection while remaining 54(18.4%) volunteers with >50 ova in per 10 ml of urine showed heavy infection.This difference was found statistically significant (X 2= 6.52, p > 0.05).The mean immunoglobulin status observed in their

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Cooper, Philip J., Ivan Espinel, Moira Wieseman, et al. "Human Onchocerciasis and Tetanus Vaccination: Impact on the Postvaccination Antitetanus Antibody Response." Infection and Immunity 67, no. 11 (1999): 5951–57. http://dx.doi.org/10.1128/iai.67.11.5951-5957.1999.

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ABSTRACT To investigate whether helminth infections may affect the efficacy of vaccines by impairing the immune response to nonparasite vaccine antigens, we compared the antibody responses to tetanus toxoid (TT) after tetanus vaccination in 193 subjects with Onchocerca volvulus infection with 85 comparable noninfected controls. After vaccination, the proportions of subjects in each group attaining protective levels of antitetanus antibodies were similar (96.9% infected versus 97.6% noninfected). Postvaccination increases in antitetanus immunoglobulin G (IgG) and the predominant IgG isotype, Ig

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Harumi Cruz, Renata, Leandro Hideki Ynoue, Carolina Sanchez Aranda, Dirceu Solé, and Antonio Condino Neto. "Antibodies to Der p 1 and Der p 2 in allergic patients." Allergologia et Immunopathologia 49, no. 2 (2021): 46–52. http://dx.doi.org/10.15586/aei.v49i2.59.

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Introduction and objectives: Atopic individuals are characterized by increased IgE production and Th2 response if exposed to certain antigens. It is known that the mother transfers antimite antibodies to the fetus and newborn, IgG thru the placenta, and IgA thru breastfeeding, but it is not clear whether there is a protective mechanism mediated by them concerning the development of future allergies. This study aimed to compare the levels of IgA, IgG, and IgE antibodies specific to Der p 1 and Der p 2 between atopic and healthy individuals.Methods: Serum samples of 98 patients and 44 healthy co

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Tandhavanant, Sarunporn, Sirikamon Koosakunirand, Taniya Kaewarpai, et al. "Longitudinal analysis to characterize classes and subclasses of antibody responses to recombinant receptor-binding protein (RBD) of SARS-CoV-2 in COVID-19 patients in Thailand." PLOS ONE 16, no. 8 (2021): e0255796. http://dx.doi.org/10.1371/journal.pone.0255796.

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Serological assays to detect antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) might contribute to confirming the suspected coronavirus disease 2019 (COVID-19) in patients not detected with molecular assays. Human antibodies that target the host angiotensin-converting enzyme 2-binding domain of the viral spike protein are a target for serodiagnosis and therapeutics. This study aimed to characterize the classes and subclasses of antibody responses to a recombinant receptor-binding protein (RBD) of SARS-CoV-2 in COVID-19 patients and investigated the reactivity of t

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Velez, Eva M. M., Carolina Maldonado Galdeano, Esteban Carmuega, Ricardo Weill, María E. Bibas Bonet, and Gabriela Perdigón. "Probiotic fermented milk consumption modulates the allergic process induced by ovoalbumin in mice." British Journal of Nutrition 114, no. 4 (2015): 566–76. http://dx.doi.org/10.1017/s0007114515001981.

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Orally administered probiotic micro-organisms are able to regulate the exacerbated immune response during the antigenic sensitisation process. The aim of the present study was to evaluate the potential efficacy of probiotic fermented milk (PFM) in preventing or treating allergy in an experimental model, and to investigate its underlying mechanisms. Ovoalbumin (OVA)-sensitised BALB/c mice were fed with PFM before the sensitisation procedure or fed continuously with PFM. At 7 and 15 d post-sensitisation, anti-OVA-specific IgE, IgG, IgG1 and IgG2a concentrations were measured in the serum and bro

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O'Brien-Simpson, Neil M., Catherine L. Black, Peter S. Bhogal, et al. "Serum Immunoglobulin G (IgG) and IgG Subclass Responses to the RgpA-Kgp Proteinase-Adhesin Complex of Porphyromonas gingivalis in Adult Periodontitis." Infection and Immunity 68, no. 5 (2000): 2704–12. http://dx.doi.org/10.1128/iai.68.5.2704-2712.2000.

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ABSTRACT Serum immunoglobulin G (IgG), IgM, and IgG subclass responses to the RgpA-Kgp proteinase-adhesin complex of Porphyromonas gingivalis were examined by enzyme-linked immunosorbent assay using adult periodontitis patients and age- and sex-matched controls. Twenty-five sera from subjects with adult periodontitis (diseased group) and 25 sera from healthy subjects (control group) were used for the study. Sera and subgingival plaque samples from 10 sites were collected from each patient at the time of clinical examination. The level of P. gingivalis in the plaque samples was determined using

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Kao, Chih-Chin, San-Yuan Wang, Yung-Kun Chuang, et al. "Clinical Mass Spectrometry Discovered Human IgG Sialylation as a Potential Biosignature for Kidney Function." Journal of Personalized Medicine 11, no. 8 (2021): 761. http://dx.doi.org/10.3390/jpm11080761.

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Immunoglobulin G (IgG) N-glycosylation was discovered to have an association with inflammation status, which has the potential to be a novel biomarker for kidney diseases. In this study, we applied an ultra-high performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS) method to plasma and urine samples from 57 individuals with different levels of kidney function. Natural abundances of total IgG, IgG1, IgG2, and IgG3 subclasses in plasma showed positive correlations to the estimated glomerular filtration rates (eGFRs). Eighteen IgG glycopeptides also showed positive correlations

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Kouvalainen, K., and I. Moilanen. "Intrapair Similarity of Immunoglobulin Levels in Twins." Acta geneticae medicae et gemellologiae: twin research 36, no. 4 (1987): 509–15. http://dx.doi.org/10.1017/s0001566000006887.

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AbstractLevels of immunoglobulins IgG, IgA, IgM and IgE were determined in 8 MZ and 14 DZ twin pairs at the ages of 6-11 years, 12-17 years and 15-20 years. Intrapair similarity in immunoglobulin levels was found to be higher in the MZ than in the DZ twins, especially in the case of immunoglobulins IgA and IgM.

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Bernasconi, Luca, Esther Mundwiler, Stephan Regenass, Vincent Aubert, Angelika Hammerer-Lercher, and Ingmar Heijnen. "Variable and inaccurate serum IgG4 levels resulting from lack of standardization in IgG subclass assay calibration." Clinical Chemistry and Laboratory Medicine (CCLM) 57, no. 11 (2019): 1777–83. http://dx.doi.org/10.1515/cclm-2019-0261.

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Abstract Background The quantification of serum IgG4 is commonly performed during the diagnostic workup of IgG4-related diseases (IgG4-RD). According to recent literature, IgG4 values above 1.35 g/L are characteristic of IgG4-RD and support its diagnosis at initial presentation. The purpose of this study was to evaluate comparability and accuracy of the two main commercially available IgG4 assays (Siemens Healthineers and The Binding Site). Methods Method comparison was performed for IgG and IgG subclasses using a collective of selected samples with elevated serum IgG4. In addition, we assesse

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Vaglio, Augusto, Johanna D. Strehl, Bernhard Manger, et al. "IgG4 immune response in Churg–Strauss syndrome." Annals of the Rheumatic Diseases 71, no. 3 (2011): 390–93. http://dx.doi.org/10.1136/ard.2011.155382.

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ObjectiveT-helper type 2 responses are crucial in Churg–Strauss syndrome (CSS) and may enhance the production of IgG4 antibodies. The authors assessed the IgG4 immune response in CSS patients.MethodsThe authors included 46 consecutive patients with CSS (24 with active and 22 with quiescent disease), 26 with granulomatosis with polyangiitis (GPA, Wegener's), 25 with atopic asthma and 20 healthy controls and determined serum IgG, IgM, IgA, IgE and IgG subclass levels. Tissue infiltration by IgG4 plasma cells was assessed in nine patients with CSS, 10 with GPA, 22 with chronic sinusitis (11 with

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Efimov, A. S., D. Ch Tajieva, and I. N. Pishel. "Immune mechanisms of development of diabetic nephropathy." Problems of Endocrinology 46, no. 5 (2000): 6–10. http://dx.doi.org/10.14341/probl11868.

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Changes in the levels of circulating immunoglobulins and production of autoantibodies to basal membrane of renal glomeruli were studied in patients with insulin-dependent diabetes mellitus (IDDM) at various stages of diabetic nephropathy (DN). The number of patients with positive reaction to autoantibodies to renal basal membrane (R.BM) increases as clinical symptoms of DN augment. The greater part of patients with positive reaction to antibodies have stage 111-IV DN. Measurements of serum immunoglobulin concentrations in patients with DN of different degree showed maximal levels of IgG in ini

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Jaglicic, Ivana, Olivera Savic, and Bojana Rodic. "Significance of determining concentrations of subclasses of IgG and IgA: Our experience." Srpski arhiv za celokupno lekarstvo 133, Suppl. 2 (2005): 113–17. http://dx.doi.org/10.2298/sarh05s2113j.

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Selective IgG and IgA subclass (IgGsc and IgAsc) deficiencies may be either partial or complete, yet, combined subclass deficiencies are common. The serums of 65 patients with various clinical symptoms were investigated for quantitative and qualitative immunoglobulin levels. A high correlation (r=0.93) between the two methods used for measuring total IgG concentration (nephelometry) and IgGsc concentration (RID measurement) was demonstrated. Patients were divided into groups according to IgG and IgA class and subclass concentrations. The serums of 51 patients were investigated for IgGsc defici

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Kralickova, P., J. Kuhnova, O. Soucek, et al. "Antibodies against Pneumococcal Capsular Polysaccharides and Natural Anti-Galactosyl (Alpha-Gal) in Patients with Humoral Immunodeficiencies." Journal of Immunology Research 2017 (2017): 1–8. http://dx.doi.org/10.1155/2017/7304658.

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Humoral deficiencies represent a broad group of disorders. The aim of the study was to compare the levels of antibodies against pneumococcal capsular polysaccharides (anti-PCP) and natural anti-galactosyl (anti-Gal) antibodies in (1) patients with chronic lymphocytic leukaemia (CLL), (2) patients with common variable immunodeficiency (CVID), and (3) a healthy population and to explore their diagnostic and prognostic potential. Serum immunoglobulin levels and levels of anti-Gal IgG, IgA, and IgM and anti-PCP IgG and IgG2 were determined in 59 CLL patients, 30 CVID patients, and 67 healthy contr

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John, Chandy C., Joseph S. Zickafoose, P. Odada Sumba, Christopher L. King, and James W. Kazura. "Antibodies to the Plasmodium falciparum Antigens Circumsporozoite Protein, Thrombospondin-Related Adhesive Protein, and Liver-Stage Antigen 1 Vary by Ages of Subjects and by Season in a Highland Area of Kenya." Infection and Immunity 71, no. 8 (2003): 4320–25. http://dx.doi.org/10.1128/iai.71.8.4320-4325.2003.

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ABSTRACT Immunoglobulin G (IgG) antibodies to three vaccine candidate preerythrocytic Plasmodium falciparum antigens were evaluated in children and adults in an epidemic-prone highland area of Kenya during rainy (high-transmission) and dry (low-transmission) seasons. The frequencies and median levels of IgG antibodies to circumsporozoite protein (CSP) and thrombospondin-related adhesive protein (TRAP) were compared to the frequencies and median levels of IgG antibodies to liver-stage antigen 1 (LSA-1) reported previously. The frequencies and median levels of IgG antibodies to CSP and TRAP were

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Chen, Dexiang, Vicki Barniak, Karl R. VanDerMeid, and John C. McMichael. "The Levels and Bactericidal Capacity of Antibodies Directed against the UspA1 and UspA2 Outer Membrane Proteins ofMoraxella (Branhamella) catarrhalis in Adults and Children." Infection and Immunity 67, no. 3 (1999): 1310–16. http://dx.doi.org/10.1128/iai.67.3.1310-1316.1999.

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ABSTRACT The UspA1 and UspA2 proteins from Moraxella catarrhalisshare antigenic epitopes and are promising vaccine candidates. In this study, the levels and bactericidal activities of antibodies in sera from healthy adults and children toward UspA1 and UspA2 from the O35E strain were measured. Human sera contained antibodies to both proteins, and the levels of immunoglobulin G (IgG) antibodies were age dependent. Adult sera had significantly higher titers of IgG than child sera (P < 0.01). The IgG3 titers to the UspA proteins were higher than the IgG1 titers in the adults’ sera, while the I

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Dechant, Michael, Gestur Vidarsson, Bernhard Stockmeyer, et al. "Chimeric IgA antibodies against HLA class II effectively trigger lymphoma cell killing." Blood 100, no. 13 (2002): 4574–80. http://dx.doi.org/10.1182/blood-2002-03-0687.

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Antibodies against human leukocyte antigen (HLA) class II, such as 1D10 or Lym-1, are currently being evaluated for the treatment of B-cell lymphomas. Previous studies have demonstrated that, in addition to IgG Fc receptors, the human myeloid IgA receptor (FcαRI, CD89) also effectively triggered tumor cell killing. Therefore, we used the variable light and heavy chain sequences from another murine anti–HLA class II hybridoma, F3.3, to generate a panel of chimeric human/mouse antibodies, including human immunoglobulin A1 (IgA1), IgA2, IgG1, IgG2, IgG3, and IgG4. Antibody production was accompli

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Laranjeira, Daniela Farias, Vânia Lúcia Ribeiro da Matta, Thaíse Yumie Tomokane, Mary Marcondes, Carlos Eduardo Pereira Corbet, and Márcia Dalastra Laurenti. "Serological and infection statuses of dogs from a visceral leishmaniasis-endemic area." Revista de Saúde Pública 48, no. 4 (2014): 563–71. http://dx.doi.org/10.1590/s0034-8910.2014048005224.

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OBJECTIVE This study investigated the serological status of dogs living in a visceral leishmaniasis-endemic area and its correlation with the parasitological condition of the animals.METHODS Canine humoral response was evaluated using the sera of 134 dogs by enzyme-linked immunosorbent assay and immunohistochemistry to detect parasites in the skin, lymph node, and spleen of the animals. The specific antibodies investigated were IgG, IgG1, IgG2, and IgE.RESULTS According to the parasitological, laboratory, and clinical findings, the dogs were placed into one of four groups: asymptomatic with (A

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Barton, James C., Luigi F. Bertoli, and J. Clayborn Barton. "Comparisons of CVID and IgGSD: Referring Physicians, Autoimmune Conditions, Pneumovax Reactivity, Immunoglobulin Levels, Blood Lymphocyte Subsets, and HLA-A and -B Typing in 432 Adult Index Patients." Journal of Immunology Research 2014 (2014): 1–10. http://dx.doi.org/10.1155/2014/542706.

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Common variable immunodeficiency (CVID) and immunoglobulin (Ig) G subclass deficiency (IgGSD) are heterogeneous disorders characterized by respiratory tract infections, selective Ig isotype deficiencies, and impaired antibody responses to polysaccharide antigens. Using univariable analyses, we compared observations in 34 CVID and 398 IgGSD adult index patients (81.9% women) referred to a hematology/oncology practice. Similarities included specialties of referring physicians, mean ages, proportions of women, reactivity to Pneumovax, median serum IgG3 and IgG4 levels, median blood CD56+/CD16+ ly

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Vidarsson, Gestur, Sigurveig T. Sigurdardottir, Thorolfur Gudnason, et al. "Isotypes and Opsonophagocytosis of Pneumococcus Type 6B Antibodies Elicited in Infants and Adults by an Experimental Pneumococcus Type 6B-Tetanus Toxoid Vaccine." Infection and Immunity 66, no. 6 (1998): 2866–70. http://dx.doi.org/10.1128/iai.66.6.2866-2870.1998.

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ABSTRACT Streptococcus pneumoniae is a major respiratory pathogen of infants, children, and the elderly. Polysaccharide vaccines have been useful in adult populations but do not elicit protective immunity in infants and young children. To enhance their immunogenicity, vaccines of pneumococcal polysaccharides conjugated to proteins are being developed. In this study antibody levels and opsonic activities were compared in sera of infants and adults injected with pneumococcal polysaccharide type 6B (Pn6B) conjugated to tetanus toxoid (TT) (Pn6B-TT). Healthy infants were injected with Pn6B-TT; gro

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Vermont, C. L., H. H. van Dijken, C. J. P. van Limpt, R. de Groot, L. van Alphen, and G. P. J. M. van den Dobbelsteen. "Antibody Avidity and Immunoglobulin G Isotype Distribution following Immunization with a Monovalent Meningococcal B Outer Membrane Vesicle Vaccine." Infection and Immunity 70, no. 2 (2002): 584–90. http://dx.doi.org/10.1128/iai.70.2.584-590.2002.

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ABSTRACT The avidity maturation and immunoglobulin G (IgG) isotype distribution of antibodies after vaccination with a meningococcal B outer membrane vesicle (OMV) vaccine were evaluated as indicators of protective immunity. Pre- and postvaccination sera from 134 healthy toddlers (ages, 2 to 3 years) immunized with a monovalent meningococcal B OMV (serosubtype P1.7-2,4) vaccine adsorbed with AlPO4 or Al(OH)3 were analyzed by enzyme-linked immunosorbent assay (ELISA) methods. The children were vaccinated three times with intervals of 3 to 6 weeks between vaccinations or twice with an interval o

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Neyestani, Tirang R., and Bill Woodward. "Blood Concentrations of Th2-Type Immunoglobulins are Selectively Increased in Weanling Mice Subjected to Acute Malnutrition." Experimental Biology and Medicine 230, no. 2 (2005): 128–34. http://dx.doi.org/10.1177/153537020523000207.

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Male and female C57BL/6J mice, initially 19 days old, consumed a complete purified diet either ad libitum (age-matched control) or in restricted daily quantities (energy deficiency), or they consumed a purified isocaloric low-protein diet ad libitum (protein and energy deficit). In a 14-day experimental period, malnourished animals lost approximately 1.5% of their initial body weight daily. Zero-time controls, 19 days old, were also included in the study. Serum levels of Th2-type (IgG1 and IgE) and Th1-type (IgG2a and IgG3) immunoglobulins were quantified by enzyme-linked immunosorbent assay,

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Stelmach, Iwona, Malgorzata Podsiadłowicz-Borzęcka, Tomasz Grzelewski, et al. "Humoral and Cellular Immunity in Children with Mycoplasma pneumoniae Infection: a 1-Year Prospective Study." Clinical Diagnostic Laboratory Immunology 12, no. 10 (2005): 1246–50. http://dx.doi.org/10.1128/cdli.12.10.1246-1250.2005.

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ABSTRACT To determine whether children have persistent abnormalities in cellular and humoral immunity development after acute Mycoplasma pneumoniae infection, serum immunoglobulin G (IgG), IgA, IgM, and IgE levels and lymphocyte phenotypes were determined. There were no changes in the levels of IgG, IgM, IgA, or CD4+ or CD19+ lymphocytes that were measured in M. pneumoniae-positive patients after 3 months or after 12 months, but there were increases in these in M. pneumoniae-negative patients. Serum IgE increased in M. pneumoniae-positive patients. We have shown alterations in immunity develop

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Lobos, Edgar, Thomas B. Nutman, John S. Hothersall та Salvador Moncada. "Elevated Immunoglobulin E against Recombinant Brugia malayi γ-Glutamyl Transpeptidase in Patients with Bancroftian Filariasis: Association with Tropical Pulmonary Eosinophilia or Putative Immunity". Infection and Immunity 71, № 2 (2003): 747–53. http://dx.doi.org/10.1128/iai.71.2.747-753.2003.

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ABSTRACT A major allergen of the lymphatic filarial nematode Brugia malayi, a homologue of γ-glutamyl transpeptidase (γ-GT), is involved in the pathology of tropical pulmonary eosinophilia (TPE) through its potent allergenicity and the induction of antibodies against the host pulmonary epithelium. To investigate the immunoglobulin G (IgG) subclass and IgE responses to recombinant B. malayi γ-GT, we analyzed the results obtained from 51 patients with differing clinical manifestations of bancroftian filariasis. γ-GT-specific IgG1, rather than IgG4, was the predominant IgG subclass, particularly

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Megnekou, Rosette, Trine Staalsoe, Diane W. Taylor, Rose Leke, and Lars Hviid. "Effects of Pregnancy and Intensity of Plasmodium falciparum Transmission on Immunoglobulin G Subclass Responses to Variant Surface Antigens." Infection and Immunity 73, no. 7 (2005): 4112–18. http://dx.doi.org/10.1128/iai.73.7.4112-4118.2005.

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ABSTRACT Placenta-sequestering Plasmodium falciparum involved in the pathogenesis of pregnancy-associated malaria (PAM) in otherwise clinically immune women expresses particular variant surface antigens (VSAPAM) on the surface of infected erythrocytes that differ from VSA found in parasitized nonpregnant individuals (non-PAM type VSA). We studied levels of immunoglobulin G (IgG) and IgG subclasses with specificity for VSAPAM and for non-PAM type VSA in pregnant and nonpregnant women from two sites with different endemicities in Cameroon. We found that VSAPAM-specific responses depended on the

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Siman, Isabella Lima, Lais Martins de Aquino, Leandro Hideki Ynoue, et al. "Allergen-Specific IgG Antibodies Purified from Mite-Allergic Patients Sera Block the IgE Recognition ofDermatophagoides pteronyssinusAntigens: AnIn VitroStudy." Clinical and Developmental Immunology 2013 (2013): 1–11. http://dx.doi.org/10.1155/2013/657424.

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One of the purposes of specific immunotherapy (SIT) is to modulate humoral immune response against allergens with significant increases in allergen-specific IgG levels, commonly associated with blocking activity. The present study investigatedin vitroblocking activity of allergen-specific IgG antibodies on IgE reactivity toDermatophagoides pteronyssinus(Dpt) in sera from atopic patients. Dpt-specific IgG antibodies were purified by ammonium sulfate precipitation followed by protein-G affinity chromatography. Purity was checked by SDS-PAGE and immunoreactivity by slot-blot and immunoblot assays

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Bettoni, Giuseppe, Luca A. Lotta, Dario Consonni, et al. "ADAMTS13 Activity and Autoantibodies Subclasses as Recurrency Risk Predictors In Acquired Thrombotic Thrombocytopenic Purpura." Blood 116, no. 21 (2010): 2532. http://dx.doi.org/10.1182/blood.v116.21.2532.2532.

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Abstract Abstract 2532 Background: Thrombotic thrombocytopenic purpura (TTP) is a rare, life-threatening disease characterized by acute episodes of thrombocytopenia and microangiopathic hemolytic anemia due to disseminated microvascular thrombosis. Up to 40% of patients with TTP who survive the first acute disease episode develop one or more recurrent episodes. The severe deficiency of the von Willebrand factor (VWF) cleaving protease ADAMTS13 in plasma and the presence of anti-ADAMTS13 autoantibodies during both acute presentation and disease remission are associated with increased risk for r

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Gao, Meili, Yongfei Li, Aqun Zheng, Xiaochang Xue, Lan Chen, and Yu Kong. "Lymphocyte Oxidative Stress/Genotoxic Effects Are Related to Serum IgG and IgA Levels in Coke Oven Workers." Scientific World Journal 2014 (2014): 1–10. http://dx.doi.org/10.1155/2014/801346.

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We investigated oxidative stress/genotoxic effects levels, immunoglobulin levels, polycyclic aromatic hydrocarbons (PAHs) levels exposed in 126 coke oven workers and in 78 control subjects, and evaluated the association between oxidative stress/genotoxic effects levels and immunoglobulin levels. Significant differences were observed in biomarkers, including 1-hydroxypyrene levels, employment time, percentages of alcohol drinkers, MDA, 8-OHdG levels, CTL levels and CTM, MN, CA frequency, and IgG, IgA levels between the control and exposed groups. Slightly higher 1-OHP levels in smoking users we

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Borochova, Kristina, Katarzyna Niespodziana, Katarina Stenberg Hammar, et al. "Features of the Human Antibody Response against the Respiratory Syncytial Virus Surface Glycoprotein G." Vaccines 8, no. 2 (2020): 337. http://dx.doi.org/10.3390/vaccines8020337.

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Respiratory syncytial virus (RSV) infections are a major cause of serious respiratory disease in infants. RSV occurs as two major subgroups A and B, which mainly differ regarding the surface glycoprotein G. The G protein is important for virus attachment and G-specific antibodies can protect against infection. We expressed the surface-exposed part of A2 strain-derived G (A2-G) in baculovirus-infected insect cells and synthesized overlapping peptides spanning complete A2-G. The investigation of the natural IgG response of adult subjects during a period of one year showed that IgG antibodies (i)

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Sandor, M., T. Gajewski, J. Thorson, J. D. Kemp, F. W. Fitch, and R. G. Lynch. "CD4+ murine T cell clones that express high levels of immunoglobulin binding belong to the interleukin 4-producing T helper cell type 2 subset." Journal of Experimental Medicine 171, no. 6 (1990): 2171–76. http://dx.doi.org/10.1084/jem.171.6.2171.

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A panel of 20 murine CD4+ clones was examined for the presence of surface membrane receptors for IgA, IgM, IgD, IgE, and IgG. High level expression of multiple Fc receptors (FcRs) was found on all Th2 clones. FcR expression was low or undetected on the Th1 clones. The preferential expression of FcR on activated Th2 cells suggests potential mechanisms for immunoregulatory interactions with B cells.

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