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Journal articles on the topic 'Il Lasca'

Author: Grafiati

Published: 4 June 2021

Last updated: 2 February 2022

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1

Caro Gómez, José Antonio, Fernando Díaz del Olmo, Lorena Barba Herrero, Juan Manuel Garrido Anguita, César Borja Barrera, and José Manuel Recio Espejo. "Paleolítico Medio del Guadalquivir: las industrias de pequeñas lascas del yacimiento de Tarazona III (Sevilla, España)." SPAL. Revista de Prehistoria y Arqueología de la Universidad de Sevilla 1, no. 30 (2021): 9–45. http://dx.doi.org/10.12795/spal.2021.i30.01.

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Se analizan tecno-tipológicamente las industrias líticas, mayoritariamente elaborada sobre cuarcitas, del yacimiento paleolítico al aire libre de Tarazona III (2.479 piezas líticas), formando parte de la Secuencia Paleolítica del Guadalquivir, que abarca el final del Pleistoceno Medio e inicios del Pleistoceno Superior (129 ka y <104 ka). Se identifican las cadenas operativas del yacimiento, desde la selección de materias primas hasta la fabricación de los útiles con percutor duro. Son cadenas dirigidas especialmente a la obtención de pequeñas lascas (<5 cm), lo que refuerza la selección del tamaño del canto rodado, con patrones de reducción de núcleos elementales, buena representación de los centrípetos y baja presencia del levallois. La serie de N3 es muy equilibrada y completa; y relativamente completas las de N1 y N2 lo que sugiere, o bien la preconfiguración de las cadenas operativa en otros lugares cercanos y el transporte de los productos de talla al yacimiento; o, en N1, el desplazamiento hidro-geomorfológico de las piezas de dimensiones más reducidas. La proporción útiles lasca/lasca es del 24% (N2) y 34,7% (N3), esto es, en los niveles posteriores a 104 ka BP. Esta proporción junto a la baja variabilidad de los tipos sobre lasca, la exigua o nula presencia de macroútiles, define la industria de Tarazona III, como uno de los yacimientos representativos del Paleolítico Medio Antiguo (PMA) del Valle del Guadalquivir.

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Hecht, Nils, Johannes Woitzik, Jens P. Dreier, and Peter Vajkoczy. "Intraoperative monitoring of cerebral blood flow by laser speckle contrast analysis." Neurosurgical Focus 27, no. 4 (October 2009): E11. http://dx.doi.org/10.3171/2009.8.focus09148.

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Object Currently, reliable low-cost and noninvasive techniques to assess cerebral perfusion in the operating room are not available. The authors report on their first clinical experience with laser speckle contrast analysis (LASCA) as a complementary imaging tool for the noninvasive and dynamic assessment of cerebral blood flow (CBF) during neurovascular surgery. The purpose of this preliminary study was to address the general feasibility of LASCA in terms of handling and image quality and to provide an example of its clinical implications. Methods Laser speckle contrast analysis was performed in patients undergoing cerebral revascularization procedures for the treatment of hemodynamic compromise and complex aneurysms. The portable LASCA device was centered over the surgical field, and continuous 5-minute recordings of relative CBF were obtained. In the case of flow augmentation for hemodynamic compromise, CBF monitoring was performed before and after completion of the anastomosis. In the case of flow replacement for parent artery sacrifice, CBF monitoring was performed during consecutive 30-second test occlusions of the radial artery graft after proximal internal carotid artery sacrifice and the subsequent initiation of blood flow through the bypass. Results In all cases, the authors achieved good visualization of relative CBF in addition to flow imaging in both the bypass graft and the cortical vasculature. During a sudden CBF decrease after test occlusion of the radial artery graft and subsequent flow initiation through the bypass, LASCA allowed immediate visualization and measurement of relative CBF in excellent spatiotemporal resolution. Conclusions In this study LASCA offered noninvasive and rapid intraoperative assessment of relative CBF, which can be used for optimizing neurovascular procedures.

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Ulianova, Onega, Sergey Ulyanov, Sergey Zaytsev, Yuriy Saltykov, Alexander Ulyanov, and Valentina Feodorova. "Could LASCA-imaging of GB-speckles be applied for a high discrimination and typing of pathogenic bacteria?" PLOS ONE 16, no. 1 (January 28, 2021): e0245657. http://dx.doi.org/10.1371/journal.pone.0245657.

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In this article, the method of analysis of GB-speckles (gene-based speckles) has been adapted to the problem of detecting the differences in a group of genes (usually 5–7), used in Multi Locus Sequence Typing (MLST). This method is based on s-LASCA imaging (spatial Laser Speckle Contrast Analysis) of virtual GB-speckle and on the technique of RGB coordinates for GB-speckles, processed by the s-LASCA method. A very high sensitivity and accuracy of the new method for detecting gene polymorphism as a great alternative to classical MLST has been demonstrated. The analysis of GB-speckles, obtained for the concatenated sequences of seven genes (gatA, gidA, enoA, fumC, hemN, hflX, oppA) of three different Chlamydia trachomatis strains (E/Bour, ST94; G/9301, ST95; G/11222, ST94) has been applied as the model. The high efficiency of usage of s-LASCA-imaging of GB-speckles has been shown. The data obtained represent a significant progress in digital biology as a whole and improvements in the bio-digitalization of bacterial DNA.

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Unal-Cevik, Isin, Diclehan Orhan, Nazire Pinar Acar-Ozen, and Elmas Burcu Mamak-Ekinci. "Small Fiber Functionality in Patients with Diabetic Neuropathic Pain." Pain Medicine 22, no. 9 (April 23, 2021): 2068–78. http://dx.doi.org/10.1093/pm/pnab150.

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Abstract Objectives Diabetic neuropathic pain is associated with small fiber neuropathy. We aimed to assess the functionality of small fibers in patients with diabetes by using a practical method. Design Patients with impaired glucose tolerance (IGT), diabetic neuropathic pain (DNP), type II diabetes mellitus without neuropathic pain, and healthy control were included. Axon-reflex flare responses were induced by the intradermal application of capsaicin and histamine at the distal leg. The associated flare characteristics (flare areas and flare intensities) were recorded by using Laser Speckle Contrast Analysis (LASCA). The pain and itch responses were rated while performing LASCA. To verify the structural properties of the small fibers, proximal and distal skin biopsies were performed. Results DN4, MNSI, NRS, evoked-burning pain scores, and HbA1c levels were the highest in the DNP group. Compatible with length-dependent neuropathy, the distal skin PGP9.5-positive intraepidermal nerve fiber densities (IENFDs) were the lowest, whereas TRPV1-positive IENFDs were the highest in patients with DNP. The distal leg LASCA data showed hypo-functionality in both patients with IGT and DNP and association with disease severity. Conclusions There is an unmet need to practically assess the functionality of small fibers in patients with pain. In this study, a practical and objective method that does not need special expertise for the measurement of the functional properties of small fibers by using axon-flare responses is presented. The LASCA method could potentially facilitate a practical, quick (within 5 minutes), and very early diagnosis of small fiber hypo-functionality in both patients with IGT and DNP.

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Ulianova, Onega, Yury Saltykov, Sergey Ulyanov, Sergey Zaytsev, Alexander Ulyanov, and Valentina Feodorova. "Discrimination of the SARS–CoV-2 strains using of coloured s-LASCA-imaging of GB-speckles, developed for the gene “S” nucleotide sequences." F1000Research 10 (June 25, 2021): 503. http://dx.doi.org/10.12688/f1000research.53214.1.

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Background: A recent bioinformatics technique involves changing nucleotide sequences into 2D speckles. This technique produces speckles called GB-speckles (Gene Based speckles). All classical strategies of speckle-optics, namely speckle-interferometry, subtraction of speckle-images as well as speckle-correlometry have been inferred for processing of GB-speckles. This indicates the considerable improvement in the present tools of bioinformatics. Methods: Colour s-LASCA imaging of virtual laser GB-speckles, a new method of high discrimination and typing of pathogenic viruses, has been developed. This method has been adapted to the detecting of natural mutations in nucleotide sequences, related to the spike glycoprotein (coding the gene «S») of SARS–CoV-2 gene as the molecular target. Results: The rate of the colouring images of virtual laser GB-speckles generated by s-LASCA can be described by the specific value of R. If the nucleotide sequences compared utilizing this approach the relevant images are completely identical, then the three components of the resulting colour image will be identical, and therefore the value of R will be equal to zero. However, if there are at least minimal differences in the matched nucleotide sequences, then the value of R will be positive. Conclusion: The high effectiveness of an application of the colour images of GB-speckles that were generated by s-LASCA- has been demonstrated for discrimination between different variants of the SARS–CoV-2 spike glycoprotein gene.

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Ulianova, Onega, Yury Saltykov, Sergey Ulyanov, Sergey Zaytsev, Alexander Ulyanov, and Valentina Feodorova. "Discrimination of the SARS–CoV-2 strains using of coloured s-LASCA-imaging of GB-speckles, developed for the gene “S” nucleotide sequences." F1000Research 10 (September 6, 2021): 503. http://dx.doi.org/10.12688/f1000research.53214.2.

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Background: A recent bioinformatics technique involves changing nucleotide sequences into 2D speckles. This technique produces speckles called GB-speckles (Gene Based speckles). All classical strategies of speckle-optics, namely speckle-interferometry, subtraction of speckle-images as well as speckle-correlometry have been inferred for processing of GB-speckles. This indicates the considerable improvement in the present tools of bioinformatics. Methods: Colour s-LASCA imaging of virtual laser GB-speckles, a new method of high discrimination and typing of pathogenic viruses, has been developed. This method has been adapted to the detecting of natural mutations in nucleotide sequences, related to the spike glycoprotein (coding the gene «S») of SARS–CoV-2 gene as the molecular target. Results: The rate of the colouring images of virtual laser GB-speckles generated by s-LASCA can be described by the specific value of R. If the nucleotide sequences compared utilizing this approach the relevant images are completely identical, then the three components of the resulting colour image will be identical, and therefore the value of R will be equal to zero. However, if there are at least minimal differences in the matched nucleotide sequences, then the value of R will be positive. Conclusion: The high effectiveness of an application of the colour images of GB-speckles that were generated by s-LASCA- has been demonstrated for discrimination between different variants of the SARS–CoV-2 spike glycoprotein gene.

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Ульянова, О. В., О. С. Ребеза, and С. С. Ульянов. "ИССЛЕДОВАНИЕ ПРОЦЕССОВ РОСТА КОЛОНИЙ БАКТЕРИАЛЬНЫХ КЛЕТОК МЕТОДОМ LASCA." Оптика и спектроскопия 120, no. 1 (2016): 88–93. http://dx.doi.org/10.7868/s0030403416010232.

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Kano, Shimpei, and FABIO Z. MATSUI. "Trace elements and their address in Brazilian lasca." Journal of the Japanese Association of Mineralogists, Petrologists and Economic Geologists 82, no. 8 (1987): 299–305. http://dx.doi.org/10.2465/ganko1941.82.299.

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Ruaro, B., S. Paolino, C. Pizzorni, M. Cutolo, and A. Sulli. "Assessment of treatment effects on digital ulcer and blood perfusion by laser speckle contrast analysis in a patient affected by systemic sclerosis." Reumatismo 69, no. 3 (September 21, 2017): 134. http://dx.doi.org/10.4081/reumatismo.2017.986.

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Laser speckle contrast analysis (LASCA) is a good tool to evaluate the variation in peripheral blood perfusion during long-term follow-up and is able to safely monitor digital ulcer evolution in scleroderma patients. It evaluates blood perfusion in different areas within the skin lesions and surrounding them during standard treatment.

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Debusschere, C., A. Vanhaecke, M. Cutolo, E. Deschepper, and V. Smith. "THU0334 LASER SPECKLE CONTRAST ANALYSIS FOR MEASUREMENT OF PERIPHERAL BLOOD PERFUSION IN SYSTEMIC SCLEROSIS PATIENTS: CAN IT PREDICT FUTURE ISCHEMIC DIGITAL TROPHIC LESIONS?" Annals of the Rheumatic Diseases 79, Suppl 1 (June 2020): 398.1–398. http://dx.doi.org/10.1136/annrheumdis-2020-eular.2438.

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Background:Vasculopathy is a hallmark of systemic sclerosis (SSc). Laser speckle contrast analysis (LASCA) is a research tool to assess peripheral blood perfusion (PBP) (1). At this moment, its reliability has been attested in SSc patients, but its predictive value for future ischemic digital trophic lesions (DTL) is unknown (1).Objectives:To investigate in an unselected, prospective SSc cohort if baseline LASCA PBP measurements can discriminate between patients who will develop ischemic DTL (iDTL) and those who will not.Methods:Patients (fulfilling 2013 ACR/EULAR criteria and/or 2001 LeRoy and Medsger criteria) were recruited during the period of December 2017 to September 2018. LASCA was performed at baseline, in standardized conditions (1). Regions of interest (ROIs) (diameter 1 cm) were outlined at the 2nd-5thfingertip both volar and dorsal. The ‘average PBP’ of these ROIs was calculated (expressed in arbitrary perfusion units [PU]). A monthly telephone survey was conducted for 1 year to investigate DTL occurrence. DTL were considered ‘ischemic’ if not related to calcinosis. Logistic regression and ROC analysis were used to assess if average PBP is predictive of future iDTL.Results:Of the 106 patients with complete follow-up (92 women [86,8%]; 18 limited SSc [17,0%], 82 limited cutaneous SSc [77,4%], 6 diffuse cutaneous SSc [5,7%]), 29 patients (27,4%) had a DTL history. Forty-nine patients (46,2%) were on vasodilator therapy. Only 7 patients developed at least 1 iDTL during follow-up (6,6%) (Figure 1a). Performing univariate logistic regression (ULR), average PBP was not predictive for future iDTL (Table 1). Of note, analyzing only the patients not taking vasodilators, average PBP in the ‘iDTL group’ (n = 3) was median 46,8 PU (min. 45,6 - max. 68,8) vs. median 141,4 PU (min. 24,4 - max. 269,5) in the ‘no iDTL group’ (n = 54) (Figure 1b). In this subgroup, all 3 patients who developed iDTL (100%) had an average PBP ≤ 70 PU whereas only 9 of the 54 patients without iDTL development (16,7%) had such PBP values.Table 1.Results of ULRSummary statisticsULRVariableiDTL cases(n = 7)Non-iDTL cases(n = 99)ParameterOR(95% CI)pROC AUC(95% CI)Average PBP (PU) mean (+/- SD)123,0 (74,6)142,9 (61,9)Average PBP (linear)0,995(0,982-1,007)0,4180,597(0,352-0,843)Conclusion:In this pilot study with an unselected day-to-day SSc population, where patients were allowed to continue vasodilators, there was an unexpected low iDTL incidence, undermining the power of our study. Even though, the observations in the subgroup of patients not taking vasodilators deserve future investigation to assess whether low PBP values, as measured by LASCA, are associated with a higher iDTL incidence.References:[1]Cutolo M, Vanhaecke A, et al. Autoimmun Rev. 2018;17(8):775-80.Figure 1.Distribution of ‘average PBP’ as measured by LASCA for ‘no iDTL group’ and ‘iDTL group’Disclosure of Interests:Claire Debusschere: None declared, Amber Vanhaecke: None declared, Maurizio Cutolo Grant/research support from: Bristol-Myers Squibb, Actelion, Celgene, Consultant of: Bristol-Myers Squibb, Speakers bureau: Sigma-Alpha, Ellen Deschepper: None declared, Vanessa Smith Grant/research support from: The affiliated company received grants from Research Foundation - Flanders (FWO), Belgian Fund for Scientific Research in Rheumatic diseases (FWRO), Boehringer Ingelheim Pharma GmbH & Co and Janssen-Cilag NV, Consultant of: Boehringer-Ingelheim Pharma GmbH & Co, Speakers bureau: Actelion Pharmaceuticals Ltd, Boehringer-Ingelheim Pharma GmbH & Co and UCB Biopharma Sprl

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Bassi, Luis Felipe. "Metodologia para análise tecnológica em cristais de quartzo." Revista do Museu de Arqueologia e Etnologia, no. 25 (December 20, 2015): 105. http://dx.doi.org/10.11606/issn.2448-1750.revmae.2015.114972.

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O tema deste artigo foi discutido na dissertação de mestrado sobre a análise tecnológica do cristal de quartzo proveniente do sítio arqueológico Bibocas II, em Jequitaí/MG (Bassi, 2012). Pela avaliação dos ângulos entre as facetas naturais do cristal, identificam-se elementos importantes dos processos de lascamento, de modo que o método proposto permite localizar a lasca no suporte/núcleo e mapear possíveis métodos de lascamento.

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Briers, J. David, Glenn Richards, and Xiao Wei He. "Capillary Blood Flow Monitoring Using Laser Speckle Contrast Analysis (LASCA)." Journal of Biomedical Optics 4, no. 1 (1999): 164. http://dx.doi.org/10.1117/1.429903.

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Assunção, Danilo Chagas, Fabiana Rodrigues Belem, and Lúcia De J. C. Oliveira Juliani. "The Lithic Careará Site from São José dos Campos, SP: excavation and laboratorial analysis of a hunters-collectors site in Paraíba do Sul Valley." Revista do Museu de Arqueologia e Etnologia. Suplemento, supl.11 (September 10, 2011): 83. http://dx.doi.org/10.11606/issn.2594-5939.revmaesupl.2011.113539.

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A partir do estudo do Sítio Arqueológico Carcará, localizado em São José dos Campos/SP busca-se neste artigo, além de apresentar os dados gerados na escavação arqueológica e análise laboratorial, realizar um a discussão de um dos raros sítios relacionados à ocupação do Vale do Rio Paraíba por grupos pré-ceramistas. Localizado em área de impacto direto de em preendimento modificador do meio físico, o sítio foi alvo de trabalho de resgate arqueológico realizado pela A Lasca Consultoria

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Wu, Cong, Nengyun Feng, Koichi Harada, and Pengcheng Li. "A Hybrid De-Noising Method on LASCA Images of Blood Vessels." Journal of Signal and Information Processing 03, no. 01 (2012): 92–97. http://dx.doi.org/10.4236/jsip.2012.31012.

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Ulianova, O. V., O. S. Rebeza, and S. S. Ulyanov. "Investigation of growth processes in bacterial colonies by the LASCA technique." Optics and Spectroscopy 120, no. 1 (January 2016): 76–81. http://dx.doi.org/10.1134/s0030400x16010227.

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Dei Giudici, Luca, and Roberto Castricini. "Local Autologous Stem Cells Application in Rotator Cuff Repairs: “LASCA” Technique." Arthroscopy Techniques 9, no. 10 (October 2020): e1571-e1575. http://dx.doi.org/10.1016/j.eats.2020.06.022.

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Duque de Paula, Bernardo, Alejandra Saladino, and André Luis Ramos Soares. "Aquisição e descarte de bens arqueológicos." Revista de Arqueologia 33, no. 3 (December 28, 2020): 171–88. http://dx.doi.org/10.24885/sab.v33i3.829.

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O presente artigo tem como objetivo refletir sobre os principais aspectos a serem considerados no que diz respeito à aquisição e descarte de bens arqueológicos, tendo como base a experiência de elaboração da Política de Acervos para o Laboratório de Arqueologia, Sociedades e Culturas das Américas (LASCA/UFSM). Nesse sentido, o estabelecimento de diretrizes para a aquisição e descarte de bens arqueológicos apresenta alguns desafios devido às singularidades (e complexidades) desses acervos, quando comparados à outras tipologias que são aqui detalhadas.

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Galhardo, Danilo Alexandre. "As cadeias operatórias de manufatura de três instrumentos líticos unifaciais." Revista de Arqueologia 29, no. 1 (July 1, 2016): 18–37. http://dx.doi.org/10.24885/sab.v29i1.441.

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Este artigo visa apresentar as cadeias operatórias de manufatura de três instrumentos líticos lascados, denominados por sua tecnomorfologia de plano-convexos (instrumentos lascados unifacialmente). A partir da análise tecnológica feita na coleção lítica do Sítio Rincão 1 – Rincão, São Paulo, sob bases conceituais da cadeia operatória e economia de matéria prima, bem como nas sequências gestuais – análise diacrítica, observaram-se evidentes semelhanças nos processos de esculpimento da massa – façonnage, no caso, suportes sobre lasca, e nas sequências de retoque que completaram a formatação e o delineamento dos bordos ativos.

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Plaisance, Michel. "L’Inquiétante tradition de La Strega de Lasca. Variantes d’auteur ou réécriture éditoriale ?" Renaissance and Reformation 41, no. 4 (2018): 43. http://dx.doi.org/10.7202/1061914ar.

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Ul'yanov, S. S., V. N. Laskavyi, Alina B. Glova, T. I. Polyanina, O. V. Ul'yanova, V. A. Fedorova, and A. S. Ul'yanov. "Adaptation of LASCA method for diagnostics of malignant tumours in laboratory animals." Quantum Electronics 42, no. 5 (May 31, 2012): 399–404. http://dx.doi.org/10.1070/qe2012v042n05abeh014885.

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Goodchild, Karen Hope. "Masaccio, Andrea del Sarto, Il Lasca, and the Sausage School of Florence." Source: Notes in the History of Art 36, no. 3/4 (March 2017): 178–87. http://dx.doi.org/10.1086/693941.

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Ulyanov, S., Y. Ganilova, Dan Zhu, Jianjun Qiu, Pengcheng Li, O. Ulianova, and Qingming Luo. "LASCA with a small number of scatterers: Application for monitoring of microflow." EPL (Europhysics Letters) 82, no. 1 (March 17, 2008): 18005. http://dx.doi.org/10.1209/0295-5075/82/18005.

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Brauer, Jonathan I., Iwona B. Beech, and Jan Sunner. "Mass Spectrometric Imaging Using Laser Ablation and Solvent Capture by Aspiration (LASCA)." Journal of The American Society for Mass Spectrometry 26, no. 9 (June 30, 2015): 1538–47. http://dx.doi.org/10.1007/s13361-015-1176-0.

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Jover Maestre, Francisco Javier, Francisco Morales Tomás, and Gabriel García Atiénzar. "La producción lítica tallada del asentamiento en altura del III milenio cal BC de Vilches IV (Hellín, Albacete)." Lucentum, no. 37 (December 8, 2018): 33. http://dx.doi.org/10.14198/lvcentvm2018.37.02.

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Se presenta el estudio de la industria lítica tallada del yacimiento calcolítico en altura de Vilches-IV (Torre Uchea, Hellín, Albacete). El análisis realizado permite mostrar diferentes estrategias por lo que refiere a la captación, procesado y uso de las materias primas silíceas, tanto autóctonas como alóctonas, por parte de una comunidad campesina. En este asentamiento, además de los clásicos soportes en forma de lasca y lámina con los que se configuraron desde láminas hasta puntas de flecha, cabe destacar el empleo de placas tabulares con las que se confeccionaron diferentes útiles en forma de puñal.

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Uziębło-Życzkowska, Beata, and Paweł Krzesiński. "Correlations between Left Ventricular and Left Atrial Function Assessed by Speckle Tracking Echocardiography in Patients with Treated Well-Controlled Arterial Hypertension." Cardiology Research and Practice 2021 (April 12, 2021): 1–8. http://dx.doi.org/10.1155/2021/6674081.

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Introduction. Even in patients with well-controlled arterial hypertension (AH) and without significant comorbidities, left ventricular (LV) and left atrial (LA) strain abnormalities may sometimes be found in speckle-tracking echocardiography. Therefore, the aim of this study was to investigate the correlation between LA strain and LV diastolic and systolic function in a group of patients with treated, well-controlled AH. Material and methods. LA contractile, conduit, and reservoir function, together with echocardiographic signs of LV diastolic function and LV global longitudinal strain (LV GLS), were assessed in 101 patients with treated, well-controlled AH who met the standard criteria of normal LV ejection fraction (LVEF) and normal LV diastolic function. Results. A relevant percentage of study participants presented lower than reference LV (−18.7% for LV GLS) and LA strain (32.9% for LAS during reservoir phase, LASr; −15.9% for LAS during contraction phase, LASct; and −13.9% for LAS during conduit phase, LAScd) values. Moreover, there were statistically significant differences in LA longitudinal strain (LAS) values (LASr (31.43 vs. 36.33; p = 0.0007) and LAS LAScd (−13.09 vs. −15.79; p = 0.008)) between patients with high (>the absolute value of −20%) and lower (≤the absolute value of −20%) LV GLS, confirmed by significant correlations between LASr, LAScd, and GLS. In the correlations analysis between LAS values and LV diastolic function parameters, statistical significance was obtained for the following: LASct (contraction) versus e’avg, LASct versus E/A, LASct versus A, LAScd versus e’avg, LAScd versus E/A, and LAScd versus A. Conclusions. LV and LA strain abnormalities occurred within a significant percentage of patients with treated, well-controlled AH. Impaired LA strain is associated with lower LV strain and reduced LV diastolic function parameters, reflecting both the passive and active properties of the LA.

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Briers, J. D. "Laser speckle contrast analysis (LASCA): a nonscanning, full-field technique for monitoring capillary blood flow." Journal of Biomedical Optics 1, no. 2 (1996): 174. http://dx.doi.org/10.1117/12.231359.

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Faiz, Ayesha. "Lothian Assessment for Screening Cognition in Aphasia (LASCA): A New Non Verbal Assessment of Cognition." Procedia - Social and Behavioral Sciences 217 (February 2016): 1052–62. http://dx.doi.org/10.1016/j.sbspro.2016.02.106.

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Cutolo, Maurizio, Amber Vanhaecke, Barbara Ruaro, Ellen Deschepper, Claudia Ickinger, Karin Melsens, Yves Piette, Amelia Chiara Trombetta, Filip De Keyser, and Vanessa Smith. "Is laser speckle contrast analysis (LASCA) the new kid on the block in systemic sclerosis? A systematic literature review and pilot study to evaluate reliability of LASCA to measure peripheral blood perfusion in scleroderma patients." Autoimmunity Reviews 17, no. 8 (August 2018): 775–80. http://dx.doi.org/10.1016/j.autrev.2018.01.023.

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Mongatti, Alessandro. "Uno scambio di sonetti tra il Lasca e Benedetto Varchi (e un omaggio a Luigi Alamanni)." Italique, no. XXI (November 1, 2018): 235–46. http://dx.doi.org/10.4000/italique.666.

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Limbourg, A., C. Radtke, R. Ipaktchi, and P. Vogt. "Quantitative Perfusionsanalyse durch Laser Speckle Contrast Analysis (LASCA) Perfusions-Imaging von kritisch perfundierten Geweben Quantitative Perfusion." Handchirurgie · Mikrochirurgie · Plastische Chirurgie 48, no. 06 (December 29, 2016): 354–62. http://dx.doi.org/10.1055/s-0042-115772.

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Pion, Eric, Claudia Asam, Anna-Lena Feder, Oliver Felthaus, Paul I. Heidekrueger, Lukas Prantl, Silke Haerteis, and Thiha Aung. "Laser speckle contrast analysis (LASCA) technology for the semiquantitative measurement of angiogenesis in in-ovo-tumor-model." Microvascular Research 133 (January 2021): 104072. http://dx.doi.org/10.1016/j.mvr.2020.104072.

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Ulianova, Onega, Alexander Ulyanov, Sergey Zaytsev, Yuriy Saltykov, Sergey Ulyanov, and Valentina Feodorova. "LASCA-imaging of gene-based speckles: application to detection of the gene polymorphism in a bacterial model." Laser Physics Letters 17, no. 6 (May 28, 2020): 065603. http://dx.doi.org/10.1088/1612-202x/ab8b66.

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Della Rossa, A., A. d’Ascanio, M. Cazzato, M. Mosca, and S. Bombarderi. "FRI0383 Laser speckle contrast imaging (LASCA) is a valid aid in the differential diagnosis of raynaud’s phenomenon." Annals of the Rheumatic Diseases 72, Suppl 3 (June 2013): A503.1—A503. http://dx.doi.org/10.1136/annrheumdis-2013-eular.1510.

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Zhang, Yameng, Yuemei Zhao, Weitao Li, Zhiyu Qian, and Lidong Xing. "Enhancement of microvessel in laser speckle image using gaussian kernel template." Journal of Innovative Optical Health Sciences 12, no. 02 (March 2019): 1950006. http://dx.doi.org/10.1142/s1793545819500068.

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Laser speckle contrast imaging (LSCI) is an optical imaging method, which can monitor microvascular flow variation directly without addition of any ectogenous dye. All the existing laser speckle contrast analysis (LASCA) methods are a combination of spatial and temporal statistics. In this study, we have proposed a new method, Gaussian kernel laser speckle contrast analysis (gLASCA), which processes the raw images primarily with the Gaussian kernel operator along the spatial direction of blood flow. We explored the properties of gLASCA in the simulation and animal cerebral ischemia perfusion model. Compared with the other existing speckle processing methods based on spatial, temporal, spatial-temporal or anisotropic linear structure; the present gLASCA method has a high spatial-temporal resolution to respond the change of velocity especially in microvasculature. Besides, the gLASCA method obtains approximately 10.2% and 7.1% higher contrast-to-noise ratio (CNR) over the anisotropic linear method (aLASCA) in the simulation and experiment models. For these advantages, gLASCA could be a better method for local microvascular laser speckle imaging in terms of cerebral ischemia reperfusion, spreading depression and brain injury diseases.

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Paul, Joseph S., Andreas R. Luft, Elijah Yew, and Fwu-Shan Sheu. "Imaging the development of an ischemic core following photochemically induced cortical infarction in rats using Laser Speckle Contrast Analysis (LASCA)." NeuroImage 29, no. 1 (January 2006): 38–45. http://dx.doi.org/10.1016/j.neuroimage.2005.07.019.

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Nebritova, O. A. "A study of hemodynamics and the structure of capillary blood flow using the method of laser speckle contrast analysis LASCA." Journal of Physics: Conference Series 1348 (December 2019): 012065. http://dx.doi.org/10.1088/1742-6596/1348/1/012065.

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Barroso, Ralf Braga, Thamara Cunha Nascimento Amaral, Francisco Eduardo Fonseca Delgado, and Cláudia Helena Cerqueira Mármora. "Relação entre a competência funcional da memória episódica e os fatores associados à independência funcional de idosos saudáveis." Revista Brasileira de Geriatria e Gerontologia 17, no. 4 (December 2014): 751–62. http://dx.doi.org/10.1590/1809-9823.2014.13118.

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OBJETIVO: O objetivo deste estudo foi descrever a relação entre competência funcional da memória episódica e fatores associados à independência de idosos saudáveis do município de Juiz de Fora, Minas Gerais, Brasil. MÉTODOS: Trata-se de estudo transversal, descritivo e exploratório em que 60 idosos saudáveis compuseram a amostra final. Para avaliar a capacidade funcional, foram utilizadas as escalas de Katz e de Lawton & Brody. A memória episódica foi avaliada utilizando-se teste de West & Thorn, validado e adaptado para a população brasileira por Yassuda, Lasca e Neri em 2005. A análise estatística foi realizada por meio do software estatístico Sphinx Léxica e Eureka, versão 5.0. RESULTADOS: Foi encontrada associação estatisticamente significante entre escolaridade e desempenho em tarefa de memória episódica e entre esta e três itens da escala de avaliação das atividades instrumentais de vida diária, embora inconclusiva. CONCLUSÕES: Acredita-se que esses resultados se devam ao fato de existir muitas diferenças entre os estudos com relação à metodologia empregada e a escolha dos instrumentos que avaliam tanto a memória episódica, quanto a capacidade funcional de idosos. Além disso, a hipótese de que no envelhecimento saudável existe uma capacidade de, frente às perdas graduais na memória episódica, compensá-las utilizando outras estratégias e recursos, deve receber atenção de futuras pesquisas.

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Sulli, A., B. Ruaro, F. Ravera, E. Alessandri, E. Bernero, B. Seriolo, M. A. Cimmino, G. Zampogna, and M. Cutolo. "FRI0382 Laser speckle contrast analysis (LASCA) a new method to evaluate blood perfusion in different skin areas of systemic sclerosis patients." Annals of the Rheumatic Diseases 72, Suppl 3 (June 2013): A502.3—A502. http://dx.doi.org/10.1136/annrheumdis-2013-eular.1509.

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Sherlock, Angela. "Book reviews : Ayahs, Lasca. s and Princes: Indians in Britain 1700-1947 By ROZINA VISRAM (London, Pluto Press, 1986). 304 pp. £8.95." Race & Class 28, no. 2 (October 1986): 107–8. http://dx.doi.org/10.1177/030639688602800217.

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Barsotti, Simone, Anna d’Ascanio, Venturini Valentina, Stagnaro Chiara, Bilia Silvia, Amanzi Laura, Marta Mosca, and Alessandra Della Rossa. "Is there a role for laser speckle contrast analysis (LASCA) in predicting the outcome of digital ulcers in patients with systemic sclerosis?" Clinical Rheumatology 39, no. 1 (July 17, 2019): 69–75. http://dx.doi.org/10.1007/s10067-019-04662-7.

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Ulianova, O. V., S. S. Uianov, Pengcheng Li, and Qingming Luo. "Estimation of reactogenicity of preparations produced on the basis of photoinactivated live vaccines against brucellosis and tularaemia on the organismic level. 1. Using the LASCA method." Quantum Electronics 41, no. 4 (April 30, 2011): 340–43. http://dx.doi.org/10.1070/qe2011v041n04abeh014598.

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Farrand, William R. "Archaeological Geology of North America, Norman P. Lasca and Jack Donahue (Editors), 1990, Geological Society of America Centennial Special Volume 4, x + 633 pp., $62.50 (clothbound)." Geoarchaeology 6, no. 4 (October 1991): 381–85. http://dx.doi.org/10.1002/gea.3340060407.

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Urata, Shuzo, Takeshi Noda, Yoshihiro Kawaoka, Hideyoshi Yokosawa, and Jiro Yasuda. "Cellular Factors Required for Lassa Virus Budding." Journal of Virology 80, no. 8 (April 15, 2006): 4191–95. http://dx.doi.org/10.1128/jvi.80.8.4191-4195.2006.

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ABSTRACT It is known that Lassa virus Z protein is sufficient for the release of virus-like particles (VLPs) and that it has two L domains, PTAP and PPPY, in its C terminus. However, little is known about the cellular factor for Lassa virus budding. We examined which cellular factors are used in Lassa virus Z budding. We demonstrated that Lassa Z protein efficiently produces VLPs and uses cellular factors, Vps4A, Vps4B, and Tsg101, in budding, suggesting that Lassa virus budding uses the multivesicular body pathway functionally. Our data may provide a clue to develop an effective antiviral strategy for Lassa virus.

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LASCARIS, MICHEL. "Leven met water." Tijdschrift voor Historische Geografie 4, no. 4 (January 1, 2019): 265–68. http://dx.doi.org/10.5117/thg2019.4.004.lasc.

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Living with water. The Dijkenkaart of the Netherlands De Cultural Heritage Agency made an interesting digital map (in GIS) of all the dikes in the Netherlands. This was possible by using existing digital maps, but new research was necessary to make this general overview. There was discussion about the dating of dikes, because dikes can be of medieval origin, but were adjusted over time. Besides dikes, researchers find GIS and historical information on poldermills, kolks, reclamations and pumping stations. That is why this map is called ‘Living with water’, because this information can help addressing new challenges in climate adaptation strategies dealing with water. Everyone can take a look, or download the map in GIS, on www.cultureelerfgoed.nl/onderwerpen/bronnen-en-kaarten/overzicht/levenmet-water-kaart.

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Goldsmith, C. S., H. G. Morrison, D. D. Auperin, S. G. Whitfield, and E. L. Palmer. "Vaccinia-lassa recombinant produces lassa-like inclusions." Proceedings, annual meeting, Electron Microscopy Society of America 47 (August 6, 1989): 1036–37. http://dx.doi.org/10.1017/s0424820100157164.

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Lassa virus, a member of the Arenaviridae, is the etiologic agent of Lassa fever. The virus is endemic in widespread areas of West Africa, particularly Nigeria, Liberia, and Sierra Leone. Rough estimates indicate that there are upwards of 300,000 human infections annually, resulting in approximately 5000 fatalities. A safe and effective vaccine for Lassa fever is not yet available. Recent studies in our laboratories have focused on the development of recombinant vaccinia viruses that express the nucleoprotein and envelope glycoproteins of Lassa virus as potential vaccine candidates. Tissue culture cells infected with various recombinant viruses were examined by thin-section TEH for any morphological alterations.Tissue culture cells were infected with wild-type Lassa virus or with recombinant vaccinia viruses expressing either the Lassa virus nucleoprotein (NP). the envelope glycoproteins (GPI,GP2), or NP, GPI, and GP2 in combination. Cells were fixed in 2.5% glutaraldehyde in 0.2M cacodylate buffer, postfixed in buffered 1% osmium tetroxide, and en bloc stained with 4% uranyl acetate. The samples were dehydrated and embedded in Polysciences Epon-substitute and Araldite. Sections were stained with uranyl acetate and lead citrate.

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Ossai, Edmund Ndudi, Ogah Emeka Onwe, Nnaemeka Philips Okeagu, Amarachi Laura Ugwuoru, Thankgod Kelechi Eze, and Augustine Sunday Nwede. "Knowledge and preventive practices against Lassa fever among heads of households in Abakaliki metropolis, Southeast Nigeria: A cross-sectional study." Proceedings of Singapore Healthcare 29, no. 2 (February 19, 2020): 73–80. http://dx.doi.org/10.1177/2010105819899120.

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Background: Lassa fever is associated with high morbidity and mortality. Objectives: This study aimed to determine the knowledge and preventive measures against Lassa fever among heads of households in Abakaliki metropolis, Southeast Nigeria. Methods: This was a descriptive cross-sectional study. A four-stage sampling design was used to select 420 respondents from Abakaliki metropolis. A good knowledge of Lassa fever was determined by the proportion of respondents scoring ⩾50% in 15 variables. Good preventive practices against Lassa fever were determined by the proportion of respondents obtaining a score of ⩾50% in 12 variables. Results: The mean age of respondents was 34.1±9.4 years, and the majority (56.5%) were males. Major sources of information included television (66.4%) and radio (64.3%). A minority (11.0%) consume rats at present, while 86.7% knew that rats could transmit Lassa fever to humans. The majority (60.0%) had a good knowledge of Lassa fever, while 66.4% demonstrated good preventive practices. A predictor of good knowledge included being a consumer of rats, (adjusted odds ratio (AOR)=2.3; 95% confidence interval (CI) 1.1–4.9). Predictors of good preventive practices included being in a low socio-economic class (AOR=2.1; 95% CI 1.3–3.6) and having a good knowledge of Lassa fever (AOR=10.2; 95% CI: 6.2–10.6). Conclusions: The majority of respondents had a good knowledge of Lassa fever and demonstrated good preventive practices against it. A good knowledge of Lassa fever was associated with good preventive practices. There is need for a good understanding of Lassa fever among the population, as this will improve preventive practices. Health workers have an important role to play in disseminating information concerning Lassa fever.

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Mateo, Mathieu, Stéphanie Reynard, Alexandra Journeaux, Clara Germain, Jimmy Hortion, Xavier Carnec, Caroline Picard, et al. "A single-shot Lassa vaccine induces long-term immunity and protects cynomolgus monkeys against heterologous strains." Science Translational Medicine 13, no. 597 (June 9, 2021): eabf6348. http://dx.doi.org/10.1126/scitranslmed.abf6348.

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A safe and protective Lassa virus vaccine is crucially needed in Western Africa to stem the recurrent outbreaks of Lassa virus infections in Nigeria and the emergence of Lassa virus in previously unaffected countries, such as Benin and Togo. Major challenges in developing a Lassa virus vaccine include the high diversity of circulating strains and their reemergence from 1 year to another. To address each of these challenges, we immunized cynomolgus monkeys with a measles virus vector expressing the Lassa virus glycoprotein and nucleoprotein of the prototypic Lassa virus strain Josiah (MeV-NP). To evaluate vaccine efficacy against heterologous strains of Lassa virus, we challenged the monkeys a month later with heterologous strains from lineage II or lineage VII, finding that the vaccine was protective against these strains. A second cohort of monkeys was challenged 1 year later with the homologous Josiah strain, finding that a single dose of MeV-NP was sufficient to protect all vaccinated monkeys. These studies demonstrate that MeV-NP can generate both long-lasting immune responses and responses that are able to protect against diverse strains of Lassa virus.

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Bowen, Michael D., Pierre E. Rollin, Thomas G. Ksiazek, Heather L. Hustad, Daniel G. Bausch, Austin H. Demby, Mary D. Bajani, Clarence J. Peters, and Stuart T. Nichol. "Genetic Diversity among Lassa Virus Strains." Journal of Virology 74, no. 15 (August 1, 2000): 6992–7004. http://dx.doi.org/10.1128/jvi.74.15.6992-7004.2000.

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ABSTRACT The arenavirus Lassa virus causes Lassa fever, a viral hemorrhagic fever that is endemic in the countries of Nigeria, Sierra Leone, Liberia, and Guinea and perhaps elsewhere in West Africa. To determine the degree of genetic diversity among Lassa virus strains, partial nucleoprotein (NP) gene sequences were obtained from 54 strains and analyzed. Phylogenetic analyses showed that Lassa viruses comprise four lineages, three of which are found in Nigeria and the fourth in Guinea, Liberia, and Sierra Leone. Overall strain variation in the partial NP gene sequence was found to be as high as 27% at the nucleotide level and 15% at the amino acid level. Genetic distance among Lassa strains was found to correlate with geographic distance rather than time, and no evidence of a “molecular clock” was found. A method for amplifying and cloning full-length arenavirus S RNAs was developed and used to obtain the complete NP and glycoprotein gene (GP1 and GP2) sequences for two representative Nigerian strains of Lassa virus. Comparison of full-length gene sequences for four Lassa virus strains representing the four lineages showed that the NP gene (up to 23.8% nucleotide difference and 12.0% amino acid difference) is more variable than the glycoprotein genes. Although the evolutionary order of descent within Lassa virus strains was not completely resolved, the phylogenetic analyses of full-length NP, GP1, and GP2 gene sequences suggested that Nigerian strains of Lassa virus were ancestral to strains from Guinea, Liberia, and Sierra Leone. Compared to the New World arenaviruses, Lassa and the other Old World arenaviruses have either undergone a shorter period of diverisification or are evolving at a slower rate. This study represents the first large-scale examination of Lassa virus genetic variation.

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ter Meulen, J., K. Koulemou, T. Wittekindt, K. Windisch, S. Strigl, S. Conde, and H. Schmitz. "Detection of Lassa Virus Antinucleoprotein Immunoglobulin G (IgG) and IgM Antibodies by a Simple Recombinant Immunoblot Assay for Field Use." Journal of Clinical Microbiology 36, no. 11 (1998): 3143–48. http://dx.doi.org/10.1128/jcm.36.11.3143-3148.1998.

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The nucleoprotein of Lassa virus, strain Josiah, was expressed inEscherichia coli as an N-terminally truncated, histidine-tagged recombinant protein. Following affinity purification the protein was completely denatured and spotted onto nitrocellulose membrane. A total of 1 μg of protein was applied for detection of Lassa virus antibodies (LVA) in a simple immunoblot assay. Specific anti-Lassa immunoglobulin M (IgM) antibodies could be detected by increasing the amount of protein to 5 μg. A panel of 913 serum specimens from regions in which Lassa virus was endemic and from regions in which Lassa virus was not endemic was used for evaluating the sensitivity and specificity of the LVA immunoblot in comparison to those of an indirect immunofluorescence (IIF) assay. The sera originated from field studies conducted in the Republic of Guinea (570 serum samples) and Liberia (99 serum samples), from inpatients of the clinical department of the Bernhard-Nocht-Institute, Hamburg, Germany (94 serum samples), and from healthy German blood donors (150 serum samples). In comparison to the IIF assay the LVA immunoblot assay had a specificity of 90.0 to 99.3%, depending on the origin of the specimens. The sensitivity was found to be highest for the Guinean samples (90.7%) and was lower for the Liberian samples (75%). Acute Lassa fever was diagnosed by PCR in 12 of 59 (20.3%) patients with fever of unknown origin (FUO) from the Republic of Guinea. On admission to the hospital, nine Lassa fever patients (75%) were reactive by the IgM immunoblot assay. One of the patients was infected with a new Lassa variant, which showed 10.4% variation on the amino acid level in comparison to the prototype strain of Lassa virus, Josiah. Seven PCR-negative patients were reactive by immunoblotting. The positive and negative predictive values of a single IgM immunoblot result for acute, PCR-confirmed Lassa fever were therefore 53.6 and 93.0%, respectively. Because of its high negative predictive value, a single IgM immunoblot result will be valuable for excluding acute Lassa fever for cases of FUO in areas where Lassa fever is endemic.

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Pushko, Peter, Joan Geisbert, Michael Parker, Peter Jahrling, and Jonathan Smith. "Individual and Bivalent Vaccines Based on Alphavirus Replicons Protect Guinea Pigs against Infection with Lassa and Ebola Viruses." Journal of Virology 75, no. 23 (December 1, 2001): 11677–85. http://dx.doi.org/10.1128/jvi.75.23.11677-11685.2001.

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ABSTRACT Lassa and Ebola viruses cause acute, often fatal, hemorrhagic fever diseases, for which no effective vaccines are currently available. Although lethal human disease outbreaks have been confined so far to sub-Saharan Africa, they also pose significant epidemiological concern worldwide as demonstrated by several instances of accidental importation of the viruses into North America and Europe. In the present study, we developed experimental individual vaccines for Lassa virus and bivalent vaccines for Lassa and Ebola viruses that are based on an RNA replicon vector derived from an attenuated strain of Venezuelan equine encephalitis virus. The Lassa and Ebola virus genes were expressed from recombinant replicon RNAs that also encoded the replicase function and were capable of efficient intracellular self-amplification. For vaccinations, the recombinant replicons were incorporated into virus-like replicon particles. Guinea pigs vaccinated with particles expressing Lassa virus nucleoprotein or glycoprotein genes were protected from lethal challenge with Lassa virus. Vaccination with particles expressing Ebola virus glycoprotein gene also protected the animals from lethal challenge with Ebola virus. In order to evaluate a single vaccine protecting against both Lassa and Ebola viruses, we developed dual-expression particles that expressed glycoprotein genes of both Ebola and Lassa viruses. Vaccination of guinea pigs with either dual-expression particles or with a mixture of particles expressing Ebola and Lassa virus glycoprotein genes protected the animals against challenges with Ebola and Lassa viruses. The results showed that immune responses can be induced against multiple vaccine antigens coexpressed from an alphavirus replicon and suggested the possibility of engineering multivalent vaccines based upon alphavirus vectors for arenaviruses, filoviruses, and possibly other emerging pathogens.

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